CN102498853A - Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel - Google Patents
Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel Download PDFInfo
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Abstract
The invention provides a planting and harvesting method for improving the 10-DABIII content of artificially planted laxus malrel, relates to a method of obtaining the artificially planted laxus malrels through planting, top cutting and harvesting on laxus malrel and extracting 10-DABIII in the artificially planted laxus malrel, and belongs to the technical field of laxus malrel culture. According to the method, the seeding is carried out in February to June or September to December in the first year, 5000 to 10000 laxus malrel is planted in each acre of land, twice top cutting is respectively carried out in February to June or September to December in the third year and September to December in the third year or February to June in the fourth year when the tree age is 3 or 4 and the tree height is 50 to 70cm, and the top cutting and harvesting are carried out on new branches grown after the twice top cutting. The method provided by the invention has the advantages that the problem of 10-DABIII medicine source lack is effectively solved, in addition, the efficiency of industrially extracting the 10-DABIII can also be improved, the production cost of medicines such as paclitaxel and the like is reduced, the medicine cost load of patients is reduced, and huge economic benefits and social benefits are generated.
Description
Technical field
The present invention relates to a kind of plantation and collecting method that improves artificial planted south yew 10-DABIII content; Relate to a kind of southern enqlish yew through plantation, truncation with gather and obtain tame Chinese yew and extract the wherein method of 10-DABIII, belong to the culture technique field of Chinese yew.
Background technology
Southern enqlish yew (formal name used at school Taxus chinensis var.mairei Cheng et L.K); Have another name called beautiful Chinese yew; Be national I level protective plant; Mainly to originate on the south China Yangtze river basin, often be born in the following mountain forest of height above sea level 1000～1200m, the distribution of being scattered about like the stars. the Guiyang mainly is distributed in Hua Xi, crow when, Kaiyang, Xifeng.Anhui Province mainly is distributed in crossdrift township, Shexian County, Huangshan and Huangshan District one band.The Bai Shan ancestral of Zhejiang Province also has extensive distribution in the national protection zone, and wherein the peaceful band of San Dui village, Qingyuan's landscape village to scape at most.It is an aiphyllium, about 20 meters, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground 60～100mm.Sprig alternate, leaf helical form and are given birth to, and line up two row, and bar shaped is little curved, long 1～4.5 centimetre, wide 2～5 millimeters.The spherical nut of fruit, arillus, seed oval.Dioecism, cross pollination.3～June of florescence, really 9～November of phase.In the steep cliff under scattered mountain region woods and mountain spring in 500～3500 meters of height above sea level inclines, like the dark and damp weather that cools, 11.2 ℃ of general average annual temperature, year precipitation 487.9 millimeter.
Southern enqlish yew is one of Chinese subtropics to warm temperate zone endemic element, and distribution is often arranged in broad leaved forest.Comparatively moist place, innerland, the foot of the hill is born in happiness.Stronger to climatic adaptation power, 11-16 ℃ of year samming, minimum extreme value can reach-11 ℃, and anti-dark and damp power is strong; Require the higher yellow earth of fertility, yellowish soil (acidity, subacidity soil); Can be water-fast wet, southern enqlish yew has stronger rudiment ability, sees the rudiment sprig on the trunk more; But growth fraction is slower, and shade-tolerant tree species is liked dark and damp environment.Self-sow is in the acid ground that mountain valley, small stream limit, gentle slope humus enrich, and neutral soil, calcium soil also can be grown.Drought-resistant barren, not anti-low-lying ponding.Damage by disease and insect is seldom arranged, poor growth, the life-span is long.
Southern enqlish yew not only has very high ornamental value, can also therefrom isolate natural organic-compound 10-and remove acetyl baccatin III (10-DABIII), is used to taxol biosynthesis or Docetaxel, and its molecular structure is following:
Taxol and Docetaxel have very strong lethal effect to breast cancer, oophoroma, lung cancer and leukaemia etc.; Side effect is little; Drug effect is remarkable, is the most effectively one of cancer therapy drug of discovered in recent years, and demand occupies first in existing antineoplastic market.And 10-DAB can only pass through the Chinese yew natural extract, and artificial planted south yew 10-DABIII content is very low at present.Therefore, how to improve southern enqlish yew 10-DABIII content and just become key of problem through artificial planting.Utilization of the present invention is different from the truncation and the collecting method of conventional means, has effectively improved 10-DABIII content in the southern enqlish yew.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, the plantation and the collecting method of 10-DABIII content in a kind of southern enqlish yew that can improve artificial planting is provided.
According to technical scheme provided by the invention, a kind of plantation and collecting method that improves artificial planted south yew 10-DABIII content, step is:
(1) plantation:
The collection of seed: early September to early October is collected ripe seed, and ripe seed is peony; In water, soaked 6～8 after gathering seed, smash arillus meat to pieces, clean and dry in the shade, be stored in indoor shady and cool dry place;
The selection ground of growing seedlings: select the hardwood forest or the vegetation area of printing opacity, select that soil layer is deep, short texture, many, the well-drained soil of sweat humus;
Plantation: at the Chinese yew seed of the seedling ground plantation of selecting through the vernalization processing, in sowing in 1 year 2～June, 5000～11000 southern enqlish yew seeds of every mu of ground plantation; Need the smooth surface of a wound during sowing, irritate and pass through bed water; Seed need pass through suppression, contacts with soil, covers river sand again, and thickness is 2～3 times of kind of grain; After planting split except that the forest land weeds comprehensively, the cleaning dry branches and fallen leaves, the cleaning bandwidth is 60～80cm, after seedling all sprouted, spray concentration was 1%～3% bordeaux mixture, sprayed once, and sprayed altogether 5～7 times in per 7～10 days; The seedbed of life in 2 years imposes corruption, ripe, rare night soil 2～3 times, ammonium sulfate 3～4 times, 0.02～0.04 kilogram/10m of the each consumption of ammonium sulfate
2
(2) truncation: southern enqlish yew grows to the branches and leaves of topping the 3rd year 2～June, and the height of tree is 50～70cm before the truncation; Carried out the secondary truncation to the 3rd year 9～December;
Or southern enqlish yew grows to the branches and leaves of topping the 3rd year 9～December, and the height of tree is 50～70cm before the truncation; To secondary truncation in the 4th year 2～June;
(3) gather: grow to and 9～December or the 5th year 2～June in the 4th year begin to gather newborn branches and leaves.
Behind step (2) twice truncation, when southern enqlish yew is grown up annual the Beginning of summer with impose nitrogenous, phosphorus, potassic fertilizer in the beginning of autumn of around, carry out the soil nutrient detection before the fertilising, confirm to impose all kinds of and quantity of chemical fertilizer according to testing result; The fertilizer of nitrogenous, phosphorus, potassium element comprises nitrogenous fertilizer, phosphate fertilizer and potash fertilizer, and wherein nitrogenous fertilizer is ammonium sulfate, and consumption is 0.1～0.12 kilogram/10m
2Potash fertilizer is potassium sulphate, and consumption is 0.05～0.08 kilogram/10m
2Phosphate fertilizer is calcium phosphate, and consumption is 0.03～0.08 kilogram/10m
2
Step (3) is when gathering, and the quantity of gathering of newborn branches and leaves accounts for 90%～95% of the amount of gathering then.
Selection grow seedlings ground condition following:
Soil: soil is plump, and the content of organic matter is many, and that the soil is porous is moistening, draining is good;
Landform: on non-abrupt slope or the mountain region of non-sunlight direct projection;
The water source: water condition is sufficient;
Traffic: concentrated, convenient relatively.
When in plantation, needing lifting, guarantee that root system is complete, and use blister-pack; Planting requirement: it is unsettled at the center, cave to carry seedling, and soil, a second light industry bureau carry that seedling, three is treaded, the curved roots of four ridgings, five, nest root, planting depth are that former nursery stock ground path position is deepened 4～5cm.
Adopt lamination vernalization to remove the southern enqlish yew Seed Dormancy during Chinese yew vernalization: the use volume fraction is alcohol seed soaking 0.5～1h of 0.9～0.95, uses 1: 1 cinder of volume ratio to wipe wax again; Using humidity is that 50%～60% wet sand is mixed thoroughly, puts into the cellar in pack into straw bag or the wooden case; Bury when seed 20%～30% radicle is sprouted and sow.
The present invention has following advantage: contrast with common plantation collecting method, according to method of the present invention, 10-DABIII has remarkable increase in the southern enqlish yew, and the 10-DABIII content increase when special in the newborn branches and leaves is comparatively obvious.Adopt method provided by the invention; Not only can effectively solve 10-DABIII medicine source problem of shortage, but also can improve the efficient that 10-DABIII is extracted in industrialization, reduce production of medicine costs such as taxol; Alleviate patient's expenses for medicine burden, produce huge economic benefit and social benefit.
Embodiment
Embodiment 1
(1) plantation:
The collection of seed: early September is collected ripe seed, and ripe seed is peony; In water, soaked 6 after gathering seed, smash arillus meat to pieces, clean and dry in the shade, be stored in indoor shady and cool dry place;
The selection ground of growing seedlings: select the hardwood forest or the vegetation area of printing opacity, select that soil layer is deep, short texture, many, the well-drained soil of sweat humus;
Plantation: at the Chinese yew seed of the seedling ground plantation of selecting through the vernalization processing, in December, 2007 sowing, 10300 southern enqlish yew seeds of every mu of ground plantation; Need the smooth surface of a wound during sowing, irritate and pass through bed water; Seed need pass through suppression, contacts with soil, covers river sand again, and thickness is 3 times of kind of grain; After planting split except that the forest land weeds comprehensively, the cleaning dry branches and fallen leaves, the cleaning bandwidth is 60～80cm, after seedling all sprouted, spray concentration was 1% bordeaux mixture, sprayed once, and sprayed altogether 5 times in per 7 days; The seedbed of life in 2 years imposes corruption, ripe, rare night soil 2 times, ammonium sulfate 3 times, 0.02 kilogram/10m of the each consumption of ammonium sulfate
2
(2) truncation: southern enqlish yew grows to the branches and leaves of topping on September 28th, 2010, and the height of tree is 50～70cm before the truncation; Carry out the secondary truncation on February 21st, 2011;
(3) gather: grow to and on August 26th, 2011 began to gather newborn branches and leaves.
Measure the method for 10-DABIII content:
It is 0.8cm that branches and leaves after gathering are crushed to length, mixing.Get the branches and leaves after 500g pulverizes, adding volume content is 4kg80% methyl alcohol, and the water-bath heated and stirred is extracted, and temperature is 40 degree, and extraction time is 3 hours, pours out extract after extraction finishes, and filters.Extract for the second time, add 3kg 80% methyl alcohol once more, the water-bath heated and stirred is extracted, and temperature is 40 degree, 1 hour extraction time, pour out extract, and filter.Repeat to extract 3 times for the second time.
Merging filtrate, being concentrated into does not have methyl alcohol, adds chloroform 6.9L, divides three extractions.Concentrate chloroform extraction liquid and obtain medicinal extract 2.65g, 10-DAB content is 2.13% in the use HPLC method mensuration medicinal extract, and 10-DAB content is 1.13g/kg in the branches and leaves.
Embodiment 2
Planting southerm yew and collecting method, 10-DABIII measuring method are identical with embodiment 1.
In November, 2007 sowing; 9800 southern enqlish yew saplings of every mu of ground sowing, on February 18th, 2010,10 mu of ground of 3 years living southern enqlish yews are truncation for the first time; On September 19th, 2011 is truncation for the second time; On March 21st, 2011, truncation was gathered, and the amount of gathering is 2.58 tons of branches and leaves, and 10-DAB content is 1.08g/kg in the branches and leaves.
Embodiment 3
Planting southerm yew and collecting method, 10-DABIII measuring method are identical with embodiment 1.
In December, 2007 sowing; 8900 Chinese yew saplings of every mu of ground sowing, on September 15th, 2010,10 mu of ground of 3 years living southern enqlish yews are truncation for the first time; On March 16th, 2011 is truncation for the second time; On September 16th, 2011, truncation was gathered, and the amount of gathering is 2.50 tons of branches and leaves, and 10-DAB content is 1.05g/kg in the branches and leaves.
What should explain at last is: the above is merely the preferred embodiments of the present invention; Be not limited to the present invention; Although the present invention has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. a plantation and collecting method that improves artificial planted south yew 10-DABIII content is characterized in that step is:
(1) plantation:
The collection of seed: early September to early October is collected ripe seed, and ripe seed is peony; In water, soaked 6～8 after gathering seed, smash arillus meat to pieces, clean and dry in the shade, be stored in indoor shady and cool dry place;
The selection ground of growing seedlings: select the hardwood forest or the vegetation area of printing opacity, select that soil layer is deep, short texture, many, the well-drained soil of sweat humus;
Plantation: at the Chinese yew seed of the seedling ground plantation of selecting through the vernalization processing, in sowing in 1 year 2～June, 5000～11000 southern enqlish yew seeds of every mu of ground plantation; Need the smooth surface of a wound during sowing, irritate and pass through bed water; Seed need pass through suppression, contacts with soil, covers river sand again, and thickness is 2～3 times of kind of grain; After planting split except that the forest land weeds comprehensively, the cleaning dry branches and fallen leaves, the cleaning bandwidth is 60～80cm, after seedling all sprouted, spray concentration was 1%～3% bordeaux mixture, sprayed once, and sprayed altogether 5～7 times in per 7～10 days; The seedbed of life in 2 years imposes corruption, ripe, rare night soil 2～3 times, ammonium sulfate 3～4 times, 0.02～0.04 kilogram/10m of the each consumption of ammonium sulfate
2
(2) truncation: southern enqlish yew grows to the branches and leaves of topping the 3rd year 2～June, and the height of tree is 50～70cm before the truncation; Carried out the secondary truncation to the 3rd year 9～December;
Or southern enqlish yew grows to the branches and leaves of topping the 3rd year 9～December, and the height of tree is 50～70cm before the truncation; To secondary truncation in the 4th year 2～June;
(3) gather: grow to and 9～December or the 5th year 2～June in the 4th year begin to gather newborn branches and leaves.
2. according to the plantation and the collecting method of the said raising artificial planted south yew of claim 1 10-DABIII content; It is characterized in that: behind step (2) twice truncation; Annual the Beginning of summer and impose nitrogenous, phosphorus, potassic fertilizer in the beginning of autumn of around when southern enqlish yew is grown up; Carry out soil nutrient before the fertilising and detect, confirm to impose all kinds of and quantity of chemical fertilizer according to testing result; The fertilizer of nitrogenous, phosphorus, potassium element comprises nitrogenous fertilizer, phosphate fertilizer and potash fertilizer, and wherein nitrogenous fertilizer is ammonium sulfate, and consumption is 0.1～0.12 kilogram/10m
2Potash fertilizer is potassium sulphate, and consumption is 0.05～0.08 kilogram/10m
2Phosphate fertilizer is calcium phosphate, and consumption is 0.03～0.08 kilogram/10m
2
3. according to the plantation and the collecting method of the said raising artificial planted south yew of claim 1 10-DABIII content, it is characterized in that: step (3) is when gathering, and the quantity of gathering of newborn branches and leaves accounts for 90%～95% of the amount of gathering then.
4. according to the plantation and the collecting method of the said raising artificial planted south yew of claim 1 10-DABIII content, it is characterized in that: the condition on the ground of selecting to grow seedlings is following:
Soil: soil is plump, and the content of organic matter is many, and that the soil is porous is moistening, draining is good;
Landform: on non-abrupt slope or the mountain region of non-sunlight direct projection;
The water source: water condition is sufficient;
Traffic: concentrated, convenient relatively.
5. according to the plantation and the collecting method of the said raising artificial planted south yew of claim 1 10-DABIII content, it is characterized in that: when in plantation, needing lifting, guarantee that root system is complete, and use blister-pack; Planting requirement: it is unsettled at the center, cave to carry seedling, and soil, a second light industry bureau carry that seedling, three is treaded, the curved roots of four ridgings, five, nest root, planting depth are that former nursery stock ground path position is deepened 4～5cm.
6. according to the plantation and the collecting method of the said raising artificial planted south yew of claim 1 10-DABIII content; It is characterized in that: adopt lamination vernalization to remove the southern enqlish yew Seed Dormancy during Chinese yew vernalization: the use volume fraction is alcohol seed soaking 0.5～1h of 0.9～0.95, uses 1: 1 cinder of volume ratio to wipe wax again; Using humidity is that 50%～60% wet sand is mixed thoroughly, puts into the cellar in pack into straw bag or the wooden case; Bury when seed 20%～30% radicle is sprouted and sow.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385069A (en) * | 2013-08-14 | 2013-11-13 | 中国林业科学研究院资源昆虫研究所 | Harvesting method for Yunnan taxus chinensis branches and leaves |
| CN104094765A (en) * | 2014-07-28 | 2014-10-15 | 重庆市碚圣农业科技股份有限公司 | Culture and collection method capable of improving Taxus media biomass |
| CN104403987A (en) * | 2015-01-06 | 2015-03-11 | 天津艾赛博生物技术有限公司 | Taxus chinensis cell strain with high yield of 10-DAB and application thereof |
| CN105123380A (en) * | 2015-07-21 | 2015-12-09 | 邹小龙 | Chinese yew breeding method for improving paclitaxel content of Chinese yew |
| CN107295938A (en) * | 2017-08-16 | 2017-10-27 | 桂林茗兴生物科技有限公司 | The implantation methods of Chinese yew |
| CN107360932A (en) * | 2017-08-16 | 2017-11-21 | 桂林茗兴生物科技有限公司 | A kind of implantation methods of Chinese yew |
| CN109906858A (en) * | 2019-04-12 | 2019-06-21 | 义乌博锐信息科技有限公司 | A kind of breeding method of Chinese yew |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1600069A (en) * | 2003-09-26 | 2005-03-30 | 牟新宇 | Method for planting yew |
| CN1714620A (en) * | 2005-07-07 | 2006-01-04 | 中山大学 | Method for increasing taxol content in cultivating Chinese yew |
| CN101147453A (en) * | 2007-11-01 | 2008-03-26 | 东北林业大学 | Artificial cultivation method for taxus chinenesis containing high content taxol |
-
2011
- 2011-10-21 CN CN 201110322291 patent/CN102498853B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1600069A (en) * | 2003-09-26 | 2005-03-30 | 牟新宇 | Method for planting yew |
| CN1714620A (en) * | 2005-07-07 | 2006-01-04 | 中山大学 | Method for increasing taxol content in cultivating Chinese yew |
| CN101147453A (en) * | 2007-11-01 | 2008-03-26 | 东北林业大学 | Artificial cultivation method for taxus chinenesis containing high content taxol |
Non-Patent Citations (3)
| Title |
|---|
| 吴光柳等: "南方红豆杉育苗技术", 《浙江林业科技》 * |
| 吴晓明等: "南方红豆杉种子育苗技术", 《林业科技开发》 * |
| 彭少麟等: "影响红豆杉生长的主要生态因子", 《中山大学学报(自然科学版)》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103385069A (en) * | 2013-08-14 | 2013-11-13 | 中国林业科学研究院资源昆虫研究所 | Harvesting method for Yunnan taxus chinensis branches and leaves |
| CN103385069B (en) * | 2013-08-14 | 2016-01-20 | 中国林业科学研究院资源昆虫研究所 | The collecting method of taxusyunnanensis branches and leaves |
| CN104094765A (en) * | 2014-07-28 | 2014-10-15 | 重庆市碚圣农业科技股份有限公司 | Culture and collection method capable of improving Taxus media biomass |
| CN104094765B (en) * | 2014-07-28 | 2016-06-01 | 重庆市碚圣农业科技股份有限公司 | A kind of cultivation and collection method improving Taxus x media biomass |
| CN104403987A (en) * | 2015-01-06 | 2015-03-11 | 天津艾赛博生物技术有限公司 | Taxus chinensis cell strain with high yield of 10-DAB and application thereof |
| CN105123380A (en) * | 2015-07-21 | 2015-12-09 | 邹小龙 | Chinese yew breeding method for improving paclitaxel content of Chinese yew |
| CN107295938A (en) * | 2017-08-16 | 2017-10-27 | 桂林茗兴生物科技有限公司 | The implantation methods of Chinese yew |
| CN107360932A (en) * | 2017-08-16 | 2017-11-21 | 桂林茗兴生物科技有限公司 | A kind of implantation methods of Chinese yew |
| CN109906858A (en) * | 2019-04-12 | 2019-06-21 | 义乌博锐信息科技有限公司 | A kind of breeding method of Chinese yew |
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Effective date of registration: 20220822 Address after: 214199 No. 111, Qinxin Road, Donggang Town, Xishan District, Wuxi City, Jiangsu Province Patentee after: Wuxi yew Pharmaceutical Co.,Ltd. Address before: 214199 Jiangsu Wuxi Xishan District Donggang Town Hong Kong Red Group Co., Ltd. Patentee before: JIANGSU YEW HEALTH TECHNOLOGY CO.,LTD. |