CN1714620A - Method for increasing taxol content in cultivating Chinese yew - Google Patents
Method for increasing taxol content in cultivating Chinese yew Download PDFInfo
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- CN1714620A CN1714620A CN 200510035636 CN200510035636A CN1714620A CN 1714620 A CN1714620 A CN 1714620A CN 200510035636 CN200510035636 CN 200510035636 CN 200510035636 A CN200510035636 A CN 200510035636A CN 1714620 A CN1714620 A CN 1714620A
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- chinese yew
- taxol
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- lanthanum
- yew
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Abstract
The present invention relates to cultivation method of increasing taxol content in Chinese yew. The method is to apply lanthanum containing compound as fertilizer additive under proper Chinese yew growing condition. In the root part of soil cultivated Chinese yew, lanthanum containing compound in 0.00015-0.0015 mole is applied for each plant, or, in the water culture liquid for Chinese yew, lanthanum containing compound is added to La ion concentration of 0.001-0.01 mol/L. The present invention can raise taxol content in Chinese yew and increase yield of taxane compounds with extremely high economic value. The present invention has wide application foreground.
Description
Technical field
The present invention relates to plant field, specifically relate to a kind of method that improves content of taxol in the cultivating Chinese yew.
Background technology
Chinese yew (Taxus spp.), be commonly called as " yew ", the first-grade state protection plant, contain bearing taxanes, its secondary metabolic compounds taxol is the specific drug of treatment breast cancer and oophoroma, other multiple malignant tumours such as lung cancer, Kaposi's sarcoma are also had better curative effect, are the wide spectrum of generally acknowledging in the world today, strong active anticancer medicine; Can promote the polymerization of tubulin specifically, the blocking-up cell division is the useful tool of cytology research.Taxol content in poky Chinese yew is very low, and it is very high to extract the cost that separates, and price is very expensive.Clinical and increasing day by day of scientific research demand disappears the Chinese yew in many places in large area, causes original limited natural resource deficient more.For this reason; domestic and international many government organs, pharmaceuticals and individual have dropped into a huge sum of money; set up medicinal woods; with regular collection branches and leaves; obtain taxol and chemical semi-synthetic precursors such as 10-deacetylbaccatin III, Baccatin III thereof, also the interests with the protection wild resource are consistent for this relatively stablely.
But the investment and the plantation construction of a lot of blindness have caused very serious problem.According to investigations, content of taxol is very low in the Chinese yew, and changes with the difference in kind, age, season, detect less than taxol in branches and leaves of yew of some local cultivations at home, has caused resource, financial resources, man power and material's waste.Bearing taxanes synthetic amount in Chinese yew is very little not synthetic even, and relevant enzyme activity is also very low.Therefore, can find a kind of method of content of taxol particular importance that seems that improves in the Chinese yew.So far, Shang Weiyou imposes the report that variable concentrations contains lanthanum compound to the Chinese yew little tree of cultivation.
Summary of the invention
The objective of the invention is to provides a kind of method that can improve content of taxol in the cultivating Chinese yew at the low problem of content of taxol in the above-mentioned Chinese yew.
The present invention finds to apply an amount of content that lanthanum compound can improve taxol in the Chinese yew that contains by a large amount of experimental studies under the condition of suitable cultivation Chinese yew.In soil cultivation, apply 0.00015～0.0015 mole the content that lanthanum compound can obviously improve taxol that contains at the root of every strain Chinese yew.In hydroponics, in Chinese yew water culture fluid, apply and contain lanthanum compound, make its water culture fluid La
3+Concentration is 0.001～0.01mol/L, can obviously improve the content of taxol.
Above-mentioned Chinese yew is 2～3 years living Taxus x medias.
The above-mentioned lanthanum compound that contains is lanthanum chloride or lanthanum nitrate.
No matter be to the water planting experimental group or to the experimental group of soil cultivation, the La that applies doses can significantly improve content of taxol.Thulium all can promote the growth of cell usually when suitable concentration, promote the biosynthesis from 10-deacylated tRNA Ji Bakading III to taxol and cause the rising of content of taxol, but also have inhibitory action when excessive concentrations.
La can act on multiple structural constituent as a kind of use thulium widely, and the effect of activating enzyme also further promotes cell and plant growing.La spread effect synthetic to bearing taxanes and growth of taxol resembles inorganic inducing, acts on from 10-deacylated tRNA Ji Bakading III to the biosynthesis path of taxol.
Beneficial effect of the present invention: the present invention finds to apply an amount of La by a large amount of experimental studies in the cultivating Chinese yew process
3+Can improve the content of taxol in the Chinese yew, thereby alleviate the low difficult problem of the content of taxol in the Chinese yew.Taxol has high economic worth,
Of the present invention having a extensive future.
Embodiment
Embodiment 1 soil cultivation
1. vegetable material and processing
The material of field cultivating experiment is that (little tree of Taxus * media) is planted in the Chinese yew experiment garden, Shang Cao town, county, the Qingyuan City Lianshan Mountain, Guangdong Province (height above sea level 647m) the biennial Taxus x media that little numerous Co., Ltd buys in May, 2003 from the Chengdu plant.Every plant has carried out claiming for the first time fresh weight and has grubbed out out on May 26th, 2004 that water claims fresh weight after rinsing soil matrix once more when cultivating on May 26th, 2003.The loosing soil of plantation is fertile, is fit to the growth of Chinese yew.
Experiment is provided with 14 groups of Taxus * media little tree, and 20 every group, 12 groups are used for experiment, and 2 groups are used to compare.Each experimental group has all applied the lanthanum chloride (seeing Table 1) of various dose.
In the soil cultivation of table 1 Taxus * media little tree to the lanthanum chloride amount of application of every little tree in each experimental group.
| The heavy metallic salt compound | Handle | |||
| 10 -2MLa 3+ | 10 -3MLa 3+ | 10 -4MLa 3+ | Contrast | |
| LaCl 3 | 0.0015mol/ every | 0.00015mol/ every | 0.000015mol/ every | Do not add LaCl 3 |
2 results:
Cultivated three months, the result is as shown in table 2, has applied in the Chinese yew of 0.000015mol lanthanum chloride content of taxol and has changed not quite, and content of taxol has improved 94.1% and 88.2% respectively after applying 0.0015mol lanthanum chloride and 0.00015mol lanthanum chloride.As seen, in every strain Chinese yew, apply the content that 0.0015～0.00015mol lanthanum chloride can improve taxol in the Chinese yew.
The La of table 2 various dose
3+Influence to content of taxol (mean value ± standard error) in the Taxus * media. little tree of soil cultivation
| Handle | Mean value ± standard error (taxol, n=3) |
| Earth culture | |
| 10 -4MLa 3+ 10 -3MLa 3+ 10 -2MLa 3+Contrast | 0.0109±0.0016 0.0192±0.0022 ** 0.0198±0.0026 ** 0.0102±0.0019 |
*With the t-check relatively of the control group significance of difference, significance level
*1% He
*5%.
Embodiment 2 hydroponics
1. vegetable material and processing
Biennial Taxus * media the sapling that is used for water planting experiment is that (little tree of Taxus * media) is planted in the Chinese yew experiment garden, Shang Cao town, county, the Qingyuan City Lianshan Mountain, Guangdong Province (height above sea level 647m) for the biennial Taxus x media bought from the little numerous Co., Ltd of Chengdu plant in May, 2003.The seedling of the experiment that is useful on is according to similar height, and approximate biomass screens.Rinse out soil matrix in the root system with distilled water, and reduced injury when handling as much as possible root.Then plant being transferred to 32 liters (17 (height) * 65 (length) * 29 (wide) centimetre) fills with in the plastic containers of respective handling culture fluid, pass to gas continuously, equably with air pump in the medium, be installed with 10 holes of 6 centimetres of diameters on the plastic plate of medium plastic containers.Every plant is fixed in the slotted hole with the middle rubber stopper that has aperture of riving, and the Taxus x media seedling of per two water plantings is at a distance of 10 centimetres.The plastic containers of various processing are positioned under the shade at random, and two repetitions are established in each processing, and each repeats 10 seedlings.
All water planting plant water planting all additionally apply the lanthanum chloride (seeing Table 3) of various dose according to the difference of experiment processing in Hogland culture fluid (Hogland and Arnon, 1950).For fear of salt fractional precipitation and nutrition scarcity, change culture fluid every month, and regularly add concentration and the volume of distilled water to keep culture fluid.The pH value of all nutritive solutions is to measure with Sartorius pH measuring instrument, regulates pH value to 5.5～6.0 with NaOH and HCl solution.
1 meter, water planting plant top covers with transparent plastic plate, and to cover thereon with light transmittance be the sunshade net of 40% left and right sides black again, and making it to grow in intensity of illumination is 1813.4 ± 226.7MJm
-2Year
-1Environment in.Plant from June 26,24 days to 2004 June in 2003 at ambient temperature water planting 367 days.Every plant was weighed before the water planting on June 26th, 2003, and weighed once more when gathering in the crops on June 26th, 2004.All fresh plant are clayed into power at the environment apoplexy dry doubling of aeration-drying and are used for subsequently chemical analysis.
The addition of lanthanum chloride in table 3 water planting Taxus * media. little tree Hogland culture fluid
| Handle | 10 -2M La 3+ | 10 -3M La 3+ | 10 -4M La 3+ | Blank |
| LaCl 3 | 2.45g/L | 0.245g/L | 0.0245g/L | - |
"-" expression does not add LaCl
3Processing.
2. result and conclusion
As shown in table 4, supporting the liquid chlorine la concn in water planting is that 0.0245g/L (is La
3+Concentration is 0.0001mol/L) Chinese yew in content of taxol change not quite, and support the liquid chlorine la concn when being 0.245g/L (0.001mol/L) and 2.45g/L (0.01mol/L) in water planting, content of taxol is more than three times of control group in the Chinese yew.As seen, keep Chinese yew water culture fluid lanthanum ion concentration can improve the content of taxol in the Chinese yew at 0.001～0.01mol/L.
The La of table 4 various dose
3+Influence to the Taxus * media. little tree taxol and the 10-deacylated tRNA Ji Bakading III content (mean value ± standard error) of water planting and cultivation.
| Handle | Mean value ± standard error (taxol, n=3) |
| Water planting | |
| 10 -4MLa 3+ 10 -3MLa 3+ 10 -2MLa 3+Contrast | 0.00333±0.00039 0.0101±0.0010 ** 0.0110±0.0011 ** 0.00340±0.00028 |
*With the t-check relatively of the control group significance of difference, significance level
*1% He
*5%.
Claims (5)
1, a kind of method that improves content of taxol in the cultivating Chinese yew is characterized in that under the condition of suitable cultivation Chinese yew, applies to contain lanthanum compound as fertilizer sources additive.
2, the method for claim 1 is characterized in that the root the Chinese yew of soil cultivation, and every strain applies 0.00015～0.0015 mole containing lanthanum compound or applying and contain lanthanum compound in the Chinese yew water culture fluid of hydroponics, make its water culture fluid La
3+Concentration is 0.001～0.01mol/L.
3, method as claimed in claim 2 is characterized in that described Chinese yew is 2～3 years living Chinese yew.
4, method as claimed in claim 3 is characterized in that described Chinese yew is 2～3 years living Taxus x medias.
5,, it is characterized in that the described lanthanum compound that contains is lanthanum chloride or lanthanum nitrate as the described method of claim 1～4.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510035636 CN1714620A (en) | 2005-07-07 | 2005-07-07 | Method for increasing taxol content in cultivating Chinese yew |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510035636 CN1714620A (en) | 2005-07-07 | 2005-07-07 | Method for increasing taxol content in cultivating Chinese yew |
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| CN1714620A true CN1714620A (en) | 2006-01-04 |
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| CN 200510035636 Pending CN1714620A (en) | 2005-07-07 | 2005-07-07 | Method for increasing taxol content in cultivating Chinese yew |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498853A (en) * | 2011-10-21 | 2012-06-20 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN105248228A (en) * | 2015-11-13 | 2016-01-20 | 周福进 | Taxus media cultivation method |
| CN105309141A (en) * | 2014-07-14 | 2016-02-10 | 卢鹏 | Taxus chinensis propagating method |
| CN106818256A (en) * | 2016-12-12 | 2017-06-13 | 浙江大学宁波理工学院 | Improve the regulation and control method of yew alkanes material in leaves of Taxus mairei |
| CN107295938A (en) * | 2017-08-16 | 2017-10-27 | 桂林茗兴生物科技有限公司 | The implantation methods of Chinese yew |
-
2005
- 2005-07-07 CN CN 200510035636 patent/CN1714620A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498853A (en) * | 2011-10-21 | 2012-06-20 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN102498853B (en) * | 2011-10-21 | 2013-03-13 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN105309141A (en) * | 2014-07-14 | 2016-02-10 | 卢鹏 | Taxus chinensis propagating method |
| CN105248228A (en) * | 2015-11-13 | 2016-01-20 | 周福进 | Taxus media cultivation method |
| CN106818256A (en) * | 2016-12-12 | 2017-06-13 | 浙江大学宁波理工学院 | Improve the regulation and control method of yew alkanes material in leaves of Taxus mairei |
| CN107295938A (en) * | 2017-08-16 | 2017-10-27 | 桂林茗兴生物科技有限公司 | The implantation methods of Chinese yew |
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