CN105309141A - Taxus chinensis propagating method - Google Patents
Taxus chinensis propagating method Download PDFInfo
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- CN105309141A CN105309141A CN201410333225.9A CN201410333225A CN105309141A CN 105309141 A CN105309141 A CN 105309141A CN 201410333225 A CN201410333225 A CN 201410333225A CN 105309141 A CN105309141 A CN 105309141A
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Abstract
The invention provides a taxus chinensis propagating method. The method is characterized by comprising the following steps that 1, taxus chinensis branches are selected; 2, bactericide is used for treating the branches; 3, a plant growth regulator is used for treating the branches; 4, water culture is adopted for inducing the branches to take root; 5, a nutrient solution is added for curing. By the adoption of the method for propagating taxus chinensis, restriction of cultivated land resources can be gotten rid of, and the propagation area is increased; meanwhile, the influence on the survival rate from external environment is reduced to the maximum, time for callus forming and rooting is shortened, the stem cutting survival rate is remarkably increased, the restriction that due to poor environmental factors such as temperature, humidity, soil and water quality, the stem cutting survival rate is influenced by an original propagation means is broken through, the current situation that taxus chinensis genetic resources are scanty can be relieved, and great economic value can be created.
Description
Technical field
The present invention relates to domestication of plants field, particularly, relate to the mating system of a kind of Chinese yew, especially, relate to the new mating system of a kind of rare plant China Chinese yew, be mainly used in improving the not high phenomenon of former breeding practice branch cutting survival rate.
Background technology
Taxus is in shallow rooted plant, and its main root is not obvious, side root flourishing, is generally acknowledge endangered natural rare plants for anticancer in the world, has been through the ancient few survivors seeds that quaternary glacier carries over, on earth the history of existing 2,500,000 years.
Because growth of taxol speed is slow under field conditions (factors), regeneration capacity is poor, so for a long time, has not also formed the Chinese yew raw material forestbase of advising greatly and touching in world wide.Within 1994, Chinese yew is decided to be one-level rare endangered plants by China, and had the country of Chinese yew to be called " national treasure " by 42, the whole world, the United Nations also prohibites felling, is " plant giant panda " worthy of the name simultaneously.
Chinese yew is at the equal suitable planting in various places, Chinese north and south, but growth conditions is harsh, has the advantages that happiness is shady, drought-enduring, cold-resistant, requires that P in soil H value is 5.5 ~ 7.0.The resistance to the moon of habitat property, also can grow under thick forest, perennial, does not grow into forest.Be more common in the theropencedrymion based on Korean pine.Be born on the many stone in mountain top or barren soil, many in shrub shape.The average annual temperature in original producton location 2 DEG C to 7 degree, average annual precipitation 750 ~ 1000 millimeters.How scattered under moistening, the fertile theropencedrymion of Schattenseite or half Schattenseite.Property happiness nice and cool humid climate, can the low temperature of resistance to subzero less than 30 DEG C, cold resistance is strong, optimum temperature 20 ~ 25 DEG C, genus feminine tree.Like moistening but be afraid of flood, being suitable for planting on loose moistening well-drained sand loam.
But because the bark of Chinese yew has cancer-resisting substance-taxol, have good ecological benefits and high economic worth, country is supported in proper range and builds Chinese yew woods energetically.Right Chinese yew poor growth under field conditions (factors), regeneration capacity is poor, the traditional breeding practice often envirment factor such as Yin Wendu, humidity, soil, water quality not good and cause survival rate not high, thus causes Chinese yew to hold at high price in flowers and trees market further.
Summary of the invention
The present invention is intended to overcome above-mentioned defect, provides the mating system of a kind of Chinese Chinese yew.
A mating system for Chinese yew, is mainly used in improving the not high phenomenon of former breeding practice branch cutting survival rate, it is characterized in that, comprise the following steps:
The selection of step one, Chinese yew branch;
Above-mentioned Chinese yew branch, is preferably Chinese Chinese yew branch, is selected from robust growth, biennial Chinese yew annotinous branch without damage by disease and insect; Clip instrument fire or alcohol disinfecting, the length of clip branch is within the scope of 15-30cm.
Step 2, with bactericide process branch;
The concrete technology of above-mentioned bactericide process branch is:
(1) Chinese yew branch base portion is immersed in microbicide solution carries out disinfection;
(2) the thimerosal degree of depth there was not wound, disinfecting time 15min-30min;
(3) taking-up is dried, and it is worthy of note, drying herein for suitably to dry, should be determined the degree of drying, be preferably and dry in the shade with the characteristic of plant itself.
In addition, bactericide concentration is in aqueous 1000-4000ppm; Be preferably 1000-1500ppm.
Bactericide can be selected from carbendazim, tpn, thiophanate methyl, three file ketone, triadimefon and zineb in any one.
Bactericide preferably adopts thiophanate methyl in the present invention, and its concentration is in aqueous 1000ppm.
Step 3, use plant growth regulator treatment branch;
The concrete technology of above-mentioned plant growth regulator treatment branch is:
(1), Chinese yew branch base portion is immersed in plant growth regulator solution soaks 30s-2min;
(2), take out and dry, be placed on ventilating and cooling place more than 12h, guarantee the absorption of plant growth regulator and the healing completely of wound.
In addition, plant growth regulator concentration is in aqueous 400-1000ppm;
Plant growth regulator be selected from diethyl aminoethyl hexanoate, CPPU, compound sodium nitrophenolate, brassin, gibberellin, 6-benzylaminopurine, brassinosteroid, triacontanol, indolebutyric acid, methyl α-naphthyl acetate, 2,4-D, alar-85, paclobutrazol, ethrel, paclobutrazol, excellent health azoles, chlormequat, alar-85, pik this, Triiodobenzoic acid, maleic hydrazide, powder embroider peaceful;
The gibberellin concentration preferably adopted in the present invention is 400ppm, the optimization process concentration obtained by the experiment of GA30-1000ppm process branch.
Step 4, water planting induction branch is taken root;
PH value of water solution in above-mentioned steps four should control between 5.6-6.8, adopts oxygenate apparatus, ensures the supply of oxygen; Adopt temperature control device to make greenhouse temperature maintain 20-25 DEG C, utilize the every 5-10min spraying of intermittent spraying device once, a 10-20s, keep air humidity at 85%-90%, regulate inside and outside shade net, avoid intense light irradiation direct projection at noon, and the controlled light time is at about 10-20h.
Step 5, add nutrient solution maintenance.
The above-mentioned element added in the step of nutrient solution maintenance that adds comprises N, P, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, B etc., also comprises some organic matters and inorganic chelator.
Preferably, above-mentioned element ppm concentration is in aqueous: N:400-600; P:150-200; K:260-300; Ca:70-160; Mg:80-100; Fe:3-8; Zn:0.2-1.0; Mn:0.4-2; Cu:0.01-0.1; Mo:0.01-0.1; B:0.1-1.2.
The mating system of preferred a kind of Chinese Chinese yew, is characterized in that, comprise the following steps:
Step one, the Chinese yew annotinous branch selected press 15-30cm length clip, remove top spray, with bactericide 75% thiophanate methyl 1000 times of liquid process sterilization 30min, and then dry with gibberellin immersion 30s;
Step 2, Chinese yew branch to be fixed on cystosepiment with holes, to make its base portion directly be immersed in the water induction, inside and outside shelter from heat or light, relative air humidity 85%-90%, about light application time 10h;
Step 3, after branch base portion occurs callus or grows new root, both plant can be transferred to containing maintenance in the nutritious aqueous solution as hydroponic plant, also can be transferred to the cultivation of soil mesostroma.
effect of the present invention:
Method provided by the invention is based on the superiority of water culture technology, the impact of external environment on plant propagation rate can be reduced to the full extent, whole production process is carried out artificial intelligence regulation and control, make temperature, moisture, illumination and air humidity be in optimum state all the time.
In addition, use Chinese yew of breeding of the present invention, particularly Chinese Chinese yew can keep the growth characteristics of normal plants.
In addition, method of the present invention can be used for the vegetative propagation of each Plants, and comprise the most plants such as draft class, woody, decorative flower, it is applied widely, and can realize whole year production.
It can thus be appreciated that, the inventive method is adopted to breed the restriction that Chinese yew not only can break away from cultivated land resource, expand and breed area, at utmost reduce external environment to the impact of survival rate simultaneously, Callus formation, rootage duration are shortened, allows stem cutting survival rate significantly improve, break the not good of the envirment factors such as former breeding practice Yin Wendu, humidity, soil, water quality and affect the restriction of cuttage seeding survival rate, the present situation of Chinese yew germ plasm resource scarcity can be alleviated, huge economic worth can be created again.
On the other hand, water culture technology is as a new plant cultivation technology, just in time compensate for the defect on conventional art, it has thoroughly broken away from the restriction of cultivated land resource, expands planting range, reduces the impact of external environment on reproduction rate to the full extent simultaneously, not only can break away from soil restriction, even can be manipulated by computer, factors all in growth of taxol growth course (temperature, illumination, soil, water quality) are in optimum state all the time, thus realize factorial praluction.
Embodiment
According to above-mentioned various parameter, the method for most preferred water planting China Chinese yew stem cutting is as follows:
Step one, the Chinese Chinese yew annotinous branch selected press 15-30cm length clip, remove top spray, with bactericide 75% thiophanate methyl 1000 times of liquid process sterilization 30min, and then dry with gibberellin immersion 30s;
In concrete production, flower arrangement person can carry out choosing of length as required, selects 10,30,50cm to cultivate in the present embodiment respectively.
In the present embodiment, the bactericide such as 50% tpn wetting powder, 500 times of liquid, 70% thiophanate methyl wetting powder, 800 to 1200 times of liquid, the clear 500 times of liquid of 75% white bacterium, 80% zineb, 500 times of liquid also can be adopted to carry out the screening of condition.
In the present embodiment, also can adopt concentration be in aqueous 400,500,600,800 or the gibberellin of 1000ppm, diethyl aminoethyl hexanoate, CPPU, compound sodium nitrophenolate, brassin, 6-benzylaminopurine, brassinosteroid, triacontanol, indolebutyric acid, methyl α-naphthyl acetate, 2,4-D, alar-85, paclobutrazol, ethrel, paclobutrazol, excellent health azoles, chlormequat, alar-85, pik this, Triiodobenzoic acid, maleic hydrazide, powder embroider the plant growth regulator such as peaceful and soak the screening that 30s, 1min, 1.5min, 2min etc. carry out condition.
Step 2, Chinese yew branch to be fixed on cystosepiment with holes, to make its base portion directly be immersed in the water induction, inside and outside shelter from heat or light, relative air humidity 85%-90%, about light application time 10h;
Step 3, after branch base portion occurs callus or grows new root, both plant can be transferred to containing maintenance in the nutritious aqueous solution as hydroponic plant, also can be transferred to the cultivation of soil mesostroma.
The formula of above-mentioned plant nutrition liquid is: (mg/L)
Above-mentioned plant nutrition liquid also can be the nutritional agents comprising the elements such as N, P, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, B, and particularly, above-mentioned element ppm concentration is in aqueous: N:400,500 or 600; P:150,180 or 200; K:260,280 or 300; Ca:70,90,100,120 or 160; Mg:80,90 or 100; Fe:3,5,7 or 8; Zn:0.2,0.5,0.8 or 1.0; Mn:0.4,0.6,0.9,1,1.5 or 2; Cu:0.01,0.05,0.08 or 0.1; Mo:0.01,0.05,0.08 or 0.1; B:0.1,0.5,0.8,1.0 or 1.2.
the effect of the present embodiment:
The water culture that the present embodiment adopts, compensate for the defect on conventional art, it has thoroughly broken away from the restriction of cultivated land resource, expand planting range, reduce the impact of external environment on reproduction rate to the full extent simultaneously, not only can break away from soil restriction, even can be manipulated by computer, factors all in growth of taxol growth course (temperature, illumination, soil, water quality) are in optimum state all the time, thus realize factorial praluction.
Claims (10)
1. a mating system for Chinese yew, is characterized in that, comprises the following steps:
The selection of step one, Chinese yew branch;
Step 2, with bactericide process branch;
Step 3, use plant growth regulator treatment branch;
Step 4, water planting induction branch is taken root;
Step 5, add nutrient solution maintenance.
2. the mating system of a kind of Chinese yew as claimed in claim 1, is characterized in that:
Described Chinese yew branch is selected from robust growth, biennial Chinese yew annotinous branch without damage by disease and insect;
Clip instrument fire or alcohol disinfecting, the length of clip branch is within the scope of 15-30cm.
3. the mating system of a kind of Chinese yew as claimed in claim 1, is characterized in that, the concrete technology of described bactericide process branch is:
(1) Chinese yew branch base portion is immersed in microbicide solution carries out disinfection;
(2) solution deep did not have wound, disinfecting time 15min-30min;
(3) taking-up is dried.
4. the mating system of a kind of Chinese yew as claimed in claim 3, is characterized in that:
Described bactericide concentration is in aqueous 1000-4000ppm;
Described bactericide can be selected from carbendazim, tpn, thiophanate methyl, three file ketone, triadimefon and zineb in any one.
5. the mating system of a kind of Chinese yew as claimed in claim 1, is characterized in that, the concrete technology of described plant growth regulator treatment branch is:
(1), Chinese yew branch base portion is immersed in plant growth regulator solution soaks 30s-2min;
(2), take out and dry, be placed on ventilating and cooling place more than 12h, guarantee the absorption of plant growth regulator and the healing completely of wound.
6. the mating system of a kind of Chinese yew as claimed in claim 5, is characterized in that:
Described plant growth regulator concentration is in aqueous 400-1000ppm;
Described plant growth regulator be selected from diethyl aminoethyl hexanoate, CPPU, compound sodium nitrophenolate, brassin, gibberellin, 6-benzylaminopurine, brassinosteroid, triacontanol, indolebutyric acid, methyl α-naphthyl acetate, 2,4-D, alar-85, paclobutrazol, ethrel, paclobutrazol, excellent health azoles, chlormequat, alar-85, pik this, Triiodobenzoic acid, maleic hydrazide, powder embroider peaceful.
7. the mating system of a kind of Chinese yew as claimed in claim 1, is characterized in that: the pH value of water solution in step 4 should control between 5.6-6.8, adopts oxygenate apparatus, ensures the supply of oxygen; Adopt temperature control device to make greenhouse temperature maintain 20-25 DEG C, utilize the every 5-10min spraying of intermittent spraying device once, a 10-20s, keep air humidity at 85%-90%, regulate inside and outside shade net, avoid intense light irradiation direct projection at noon, and the controlled light time is at about 10-20h.
8. the mating system of a kind of Chinese yew as claimed in claim 1, it is characterized in that, the described element added in the step of nutrient solution maintenance that adds comprises N, P, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, B etc., also comprises some organic matters and inorganic chelator.
9. the mating system of a kind of Chinese yew as claimed in claim 8, is characterized in that: described element ppm concentration is in aqueous: N:400-600; P:150-200; K:260-300; Ca:70-160; Mg:80-100; Fe:3-8; Zn:0.2-1.0; Mn:0.4-2; Cu:0.01-0.1; Mo:0.01-0.1; B:0.1-1.2.
10. the mating system of a kind of Chinese yew as described in as arbitrary in claim 1-9, is characterized in that, comprise the following steps:
Step one, the Chinese yew annotinous branch selected press 15-30cm length clip, remove top spray, with bactericide 75% thiophanate methyl 1000 times of liquid process sterilization 30min, and then dry with gibberellin immersion 30s;
Step 2, Chinese yew branch to be fixed on cystosepiment with holes, to make its base portion directly be immersed in the water induction, inside and outside shelter from heat or light, relative air humidity 85%-90%, about light application time 10h;
Step 3, after branch base portion occurs callus or grows new root, both plant can be transferred to containing maintenance in the nutritious aqueous solution as hydroponic plant, also can be transferred to the cultivation of soil mesostroma.
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Cited By (10)
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| CN105993760A (en) * | 2016-05-18 | 2016-10-12 | 合肥公盈园艺科技有限公司 | Planting method for seedlings |
| CN106069530A (en) * | 2016-06-30 | 2016-11-09 | 山东胜伟园林科技有限公司 | A kind of subtracting of high organic fertilizer is used to steam water-saving method and the application in plantation salt-soda soil photinia glabra thereof |
| CN106069496A (en) * | 2016-06-20 | 2016-11-09 | 普定县持恒林业科技发展有限公司 | A kind of Yew cuttage method for culturing seedlings |
| CN106105466A (en) * | 2016-06-30 | 2016-11-16 | 山东胜伟园林科技有限公司 | A kind of use steams water-saving method and the application in the Pollen pini thunbergii of plantation salt-soda soil thereof containing subtracting of molybdenum microorganism fertilizer |
| CN107006595A (en) * | 2017-04-17 | 2017-08-04 | 渤海大学 | A kind of storage practice of ginger |
| CN107409890A (en) * | 2017-03-23 | 2017-12-01 | 杨军 | A kind of processing method of willow cutting |
| CN107691528A (en) * | 2017-10-26 | 2018-02-16 | 桐城市天泰农业种植专业合作社 | A kind of Yew cuttage nursery soak |
| CN108841778A (en) * | 2018-04-28 | 2018-11-20 | 大连普瑞康生物技术有限公司 | A kind of yew cell tissue culture |
| CN110972952A (en) * | 2019-12-26 | 2020-04-10 | 上海帝巨智能科技有限公司 | Tissue rapid propagation culture method of Chinese yew |
| CN111837917A (en) * | 2020-07-07 | 2020-10-30 | 朗姿赛尔生物科技（广州）有限公司 | Method for soilless culture of gastrodia elata |
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| CN105993760A (en) * | 2016-05-18 | 2016-10-12 | 合肥公盈园艺科技有限公司 | Planting method for seedlings |
| CN106069496A (en) * | 2016-06-20 | 2016-11-09 | 普定县持恒林业科技发展有限公司 | A kind of Yew cuttage method for culturing seedlings |
| CN106069530A (en) * | 2016-06-30 | 2016-11-09 | 山东胜伟园林科技有限公司 | A kind of subtracting of high organic fertilizer is used to steam water-saving method and the application in plantation salt-soda soil photinia glabra thereof |
| CN106105466A (en) * | 2016-06-30 | 2016-11-16 | 山东胜伟园林科技有限公司 | A kind of use steams water-saving method and the application in the Pollen pini thunbergii of plantation salt-soda soil thereof containing subtracting of molybdenum microorganism fertilizer |
| CN107409890A (en) * | 2017-03-23 | 2017-12-01 | 杨军 | A kind of processing method of willow cutting |
| CN107006595A (en) * | 2017-04-17 | 2017-08-04 | 渤海大学 | A kind of storage practice of ginger |
| CN107691528A (en) * | 2017-10-26 | 2018-02-16 | 桐城市天泰农业种植专业合作社 | A kind of Yew cuttage nursery soak |
| CN108841778A (en) * | 2018-04-28 | 2018-11-20 | 大连普瑞康生物技术有限公司 | A kind of yew cell tissue culture |
| CN108841778B (en) * | 2018-04-28 | 2022-05-17 | 大连普瑞康生物技术有限公司 | Taxus chinensis cell tissue culture |
| CN110972952A (en) * | 2019-12-26 | 2020-04-10 | 上海帝巨智能科技有限公司 | Tissue rapid propagation culture method of Chinese yew |
| CN111837917A (en) * | 2020-07-07 | 2020-10-30 | 朗姿赛尔生物科技（广州）有限公司 | Method for soilless culture of gastrodia elata |
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Effective date of registration: 20160705 Address after: The town of Kunshan city Jiangsu province 215316 Dengyun Road No. 268, room 1 Applicant after: Suzhou Sino Vatican horticulture science and Technology Co Ltd Address before: Hangzhou City, Zhejiang province 311400 Fuyang City Dong Qiao Zhen Shang Lin Cun Ma Jia Dong No. 21 Applicant before: Lu Peng |
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Application publication date: 20160210 |