CN107360932A - A kind of implantation methods of Chinese yew - Google Patents
A kind of implantation methods of Chinese yew Download PDFInfo
- Publication number
- CN107360932A CN107360932A CN201710703317.5A CN201710703317A CN107360932A CN 107360932 A CN107360932 A CN 107360932A CN 201710703317 A CN201710703317 A CN 201710703317A CN 107360932 A CN107360932 A CN 107360932A
- Authority
- CN
- China
- Prior art keywords
- chinese yew
- soil
- seedling
- taxol
- aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of implantation methods of Chinese yew, including：1) planting environment selects；2) it is colonized, afforests：In June to December, cleaning site preparation is carried out to forest land in a manner of banding, ploughs deeply thin plough, be made into small sections of a vegetable plot shape, at the same apply abundance base manure, with plough deeply be mixed it is even, plant Chinese yew seedling, nursery stock can not tilt, root system forward horizontal development, native in covering, avoid nest root, then some are covered on the face of cave to loosen the soil, allow its it is micro- be above the ground level, prevent rainy season ponding；3) tending management：If finding that dead strain is filled in time after being colonized 1 week, field planting applies weekly 1 nutrient solution on the 1st year after surviving, and is defined so that tree root is irrigated, and timing is applied fertilizer, and is timely loosened the soil and weeding, focuses on draining, prevents treelet from being soaked by ponding, cause treelet by high temperature injury or death；4) Harmful is prevented and treated.The present invention can increase the content of taxol in Chinese yew by applying special nutrient solution to Chinese yew.
Description
Technical field
The present invention relates to field of forest cultivation technology, more particularly to a kind of implantation methods of Chinese yew.
Background technology
Taxus (Taxus L.) plant is taxaceae aiphyllium or shrub, totally 11 kinds of the whole world, is distributed in north
The temperate zone and subtropical zone of hemisphere.Alpine forest etc. has collected the higher Chinese yew of domestic and international 7 kinds of content of taxol in 1996
Kind, such as yewtree (T.brevifolia, Vancouver, CAN), Taxus x media (T.media, Canadian Wei Duoli
Sub- campus), european yew (T.baccata, Korea), taxus chinensis in northeast (T.cuspida, Northeast China), towards scarlet
Beans China fir (T.kereoensis, Korea), taxusyunnanensis (T.wallichiana, Chinese yunnan save), Xizang Taxus chinensis China fir
(Taxus wallichi-ann, Tibet, China area), it is found that Canadian yewtree taxol in bark content highest is (short
Leaf Chinese yew is that american cancer research institute finds a kind containing taxol earliest), and the taxol reported both at home and abroad so far
One kind of content highest；Taxus x media taxol in bark content is higher, is only second to yewtree.Volubility eloquence etc. is studied
Introduce a fine variety 2 Taxus medias after 9 months (4a lifes) and southerm yew (T.chinesensia var.Mairei) (20a lifes)
Blade, measurement result shows：Content of taxol is 0.051% in 4a life Taxus media blades, higher than taxusyunnanensis tree
The content (0.015-0.03%) of taxol in skin；Content of taxol is 0.017% in Taxus media blade；And 20a lifes south
Taxol is not measured in square Chinese yew blade.
It is higher with the content of taxol in taxus chinensis in northeast and taxusyunnanensis in China's taxus resource, wherein northeast
The content of 10-DAB is also higher in Taxus leaf, can be extracted and be used as semi-synthetic taxol and polyenoid Japanese yew
The precursor of alcohol.Contain higher Cephalomannine in taxusyunnanensis bark.The researchs such as Su Yingjuan are found：Southerm yew delimbing
Content of taxol is slightly less than taxus chinensis in northeast (T.cuspida) outside in bar, and all a little higher than northeast of content of taxol is red in skin and leaf
Beans China fir；Its content of taxol is significantly larger than taxusyunnanensis.Wang Daming thinks that content of taxol is less than northeast in taxusyunnanensis
Chinese yew.But Zheng De bravely thinks, content of taxol highest in taxusyunnanensis sample, the taxol average content at each position of trees
Up to 1.49x10-4, next to that southerm yew and taxus chinensis in northeast.
Foreign literature reports that taxol and its derivative content are extremely low in the Chinese yew sample of part, or even can't detect；And
Content of taxol is up to 0.05% in sample segment, and Cephalomannine content is up to 0.22%, and Baccatin content is up to 0.21%.Together
When, researcher has found that content of taxol highest sample is some rare kinds, such as Florida Chinese yew and mexican red
The high sample of beans China fir (T.floridana) .10- III contents of deacetyl baccatin be Florida Chinese yew, Taxus globosa,
Taxusyunnanensis, Canadian Chinese yew (T.canadensis), and the cultigen of several european yews.Veronika et
Al have detected the content of taxol in the various branches and leaves of yew of Hungary, the results showed that taxol contains in the branches and leaves of european yew
Measure highest.European yew is the most common Chinese yew kind in Europe, and 10- deacylation base Baccatin contents are higher in its branches and leaves.
Cheng Guangyou etc. have studied the changing rule of taxus chinensis in northeast natural population of China content of taxol, the results showed that：East
Between Taxus cuspidata colony there is significant difference in content of taxol；The amplitude of variation of content of taxol and colony between individual plant in colony
Between it is suitable, individual plant taxol highest content is 4.3 times of minimum content in the same group.It is big etc. to have studied different sources
The content of taxusyunnanensis taxol, the results showed that：The taxol in bark content interindividual variation of taxusyunnanensis is 9.4
Times.From the above we it is seen that, taxus brevifolia alcohol content fluctuation it is very big, therefore, it is difficult to carry out large area standardization kind
Plant.Cheng Guangyou etc. thinks that colony's selection or Single-plant selection Chinese yew are expected to obtain larger genetic gain.
Studied for the plantation for how improving the content of taxol in Chinese yew, it is necessary.
The content of the invention
It is an object of the invention to provide a kind of implantation methods of Chinese yew, by being continuously applied nutrition during plantation
Liquid, to reach the purpose for improving content of taxol in Chinese yew.
Technical scheme of the present invention, realized by following technical step：
A kind of implantation methods of Chinese yew, comprise the following steps：
1) planting environment：Select Guilin height above sea level 600-1000m, landform is gentle, the gradient be less than 35 °, soil layer it is loose, deep
Thick, draining facility, moistening, soil or mountain region rich in organic matter and acid soil；
2) it is colonized, afforests：In June to December, cleaning site preparation is carried out to forest land in a manner of banding, ploughs deeply and carefully ploughs, be made into
Small sections of a vegetable plot shape, while apply the base manure of abundance, it is mixed even with ploughing deeply, plants Chinese yew seedling, nursery stock can not tilt, and root system is forward horizontal
Expansion, it is native in covering, avoid nest root, then covering some on the face of cave loosens the soil, allow its it is micro- be above the ground level, prevent rainy season ponding；
3) tending management：If finding that dead strain is filled in time after being colonized 1 week, field planting applies weekly 1 nutrition on the 1st year after surviving
Liquid, it is defined so that tree root is irrigated, timing is applied fertilizer, and is timely loosened the soil and weeding, is focused on draining, is prevented treelet from being soaked by ponding, lead
Treelet is caused by high temperature injury or death；
4) Harmful is prevented and treated；
Nutrient solution described in step 3), the method for being prepared by the following procedure obtain：
1. Feedstock treating：The fresh complete stool of Chinese yew or fresh branches and leaves are cleaned up, dries, is crushed with pulverizer, with 10mm nets
Hole pulverizer crushes, grinding particle size 2-10mm；
2. extract：By step 1. obtain crush raw material add 2 times of weight methanol heating extraction, 45 DEG C extract 3 times, often
Secondary 2h, filtering merge concentrate, concentration, temperature control≤50 DEG C, and vacuum control is concentrated into 0.95- in -0.08 ± 0.01MPa
1.05g/ml, concentrated extracting solution is obtained, detect content of taxol；
3. aqueous two-phase extraction：Medicinal extract is 2. obtained to step and carries out aqueous two-phase extraction, medicinal extract and aqueous two-phase extraction liquid are according to 1:
1.25 volume ratio, the upper phase of extraction and the lower phase of extraction are obtained, double-aqueous phase system is by polyethylene glycol, chlorine used in aqueous two-phase extraction
Change sodium and water is formed, mass percent of the polyethylene glycol in the double-aqueous phase system is 15%, and sodium chloride is in the aqueous two-phase
Mass percent in system is 15%, and surplus is water, and the weight average molecular weight of the polyethylene glycol is 8000；
4. 3. aqueous phase extracted that step is obtained, milipore filter desalination of the molecular cut off for 1000-100000 is used, is obtained
Arrive.
The raw taxusyunnanensis cuttage seeding of Chinese yew seedling described in step 2) of the present invention, preferably the 2.5 of artificial culture year, ground
In more than 0.3cm, height of seedling is average in more than 50cm one-level seedling, or 1.5 years raw seedlings of southerm yew of artificial culture in footpath
The nutrient bag seedling that height of seedling is 25cm, average ground diameter is 0.08cm.
Small sections of a vegetable plot shape is made into described in step 2), preferably specification is 40cmx40cmx30cm；Described plantation, preferably seeding row spacing
From for 0.4mx1.5m, 1.65 ten thousand plants/hm of density2。
Timing fertilising described in step 3), preferably field planting apply weekly 1 cleaning up excrement water on the 1st year after surviving, apply cleaning up excrement water with handle
Tree root, which irrigates, to be defined, and applies 1 cleaning up excrement water every two weeks within the 2nd year, applies cleaning up excrement water and be defined so that tree root is irrigated, monthly applies 1 composite fertilizer,
Every plant is applied 200g, and ring is applied.
Nutrient solution described in step 3), preferably dilute 80-100 times and use.
Tending management described in step 3), refer to that the seedling just planted is small, nursery stock is not high, slow-growing, it is necessary to strengthens
Cultivating and managing, promote its growth of growing up strong and sturdy, first, it is noted that timely loosen the soil and weeding, in August to October, for it is optimal loosen the soil,
The weeding time, it is careful not to injure treelet, second, to focus on draining, prevents treelet from being soaked by ponding, cause treelet by hyperthermal injury
Evil is dead.
Harmful preventing and treating described in step 4), refers to that rhizome maize ear rot and rotten easily occurs at rainy season for Chinese yew
Root, sprinkling 500-800 times of liquid of 70% fenaminosulf on time are on the defensive and treated；And easily occur that leaf tangerine is sick, red tangerine in dry season
Disease etc., sprinkling prevention is carried out with 1% Bordeaux mixture；For the phenomenon of locust food leaf, 500-800 times of metrifonate is timely sprayed
Liquid is defendd；For the harm of snail, trapped to tag and feed with snail jinx and killed, prohibit the use of DDT etc. have high residue, it is carcinogenic,
Severe toxicity, the agricultural chemicals of slow poison carry out the prevention and control of plant diseases, pest control.
Compared with prior art, the invention has the advantages that：
1st, it is higher with the content of taxol in taxus chinensis in northeast and taxusyunnanensis in China's taxus resource, southern red
Content of taxol in beans China fir is relatively low, about 0.01-0.07%, average 0.015% (mass fraction).It is purple in different Chinese yew
The content and the age of tree of China fir alcohol, temperature, humidity, illumination, planting site height above sea level etc. have relation.The present invention is special by applying to Chinese yew
Other nutrient solution, the content of taxol in Chinese yew can be increased.
2nd, in the prior art, taxol is industrially slightly carried more using the branches and leaves of yew crushed after methanol extracts repeatedly,
Medicinal extract is condensed into, is extracted with dichloromethane, chloroform, ethyl acetate, petroleum ether, ethyl acetate etc., by such extraction,
Obtained organic phase sends to isolation of taxol, and an obtained other phase contains methanol, can not be used for Chinese yew as nutrient solution
Cultivation.Process waste of the nutrient solution of the present invention after applicant's extraction taxol, applicant extract taxol, used
After aqueous two-phase extraction, obtained organic phase, the step of into column chromatography for separation taxol, and after aqueous two-phase extraction, obtained water
Phase, discharged after being originally processing, behind applicant attempt the plantation that is used for taxol, as a result effect is fine, not only can be with
The content of taxol in Chinese yew is improved, sewage discharge can also be reduced, killed two birds with one stone.
Embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment：
A kind of implantation methods of Chinese yew, comprise the following steps：
1) planting environment：Select Guilin height above sea level 600-1000m, landform is gentle, the gradient be less than 35 °, soil layer it is loose, deep
Thick, draining facility, moistening, soil or mountain region rich in organic matter and acid soil；
2) it is colonized, afforests：In June to December, cleaning site preparation is carried out to forest land in a manner of banding, ploughs deeply and carefully ploughs, be made into
Small sections of a vegetable plot shape, specification 40cmx40cmx30cm, plant distance is 0.4mx1.5m, 1.65 ten thousand plants/hm of density2；Abundance is applied simultaneously
Base manure, with even, the plantation Chinese yew seedling that is mixed, 2.5 years raw taxusyunnanensis cuttage seedings of artificial culture is ploughed deeply, ground diameter exists
More than 0.3cm, height of seedling is in more than 50cm one-level seedling, or 1.5 years raw seedlings of southerm yew of artificial culture, average height of seedling
The nutrient bag seedling for being 0.08cm for 25cm, average ground diameter, nursery stock can not tilt, root system forward horizontal development, soil in covering, avoid
Nest root, then cover some on the face of cave and loosen the soil, allow its it is micro- be above the ground level, prevent rainy season ponding；
3) tending management：If finding that dead strain is filled in time after being colonized 1 week, field planting applies weekly 1 nutrition on the 1st year after surviving
Liquid, it is defined so that tree root is irrigated, timing is applied fertilizer, and field planting applies weekly 1 cleaning up excrement water on the 1st year after surviving, and applies cleaning up excrement water with tree root
Irrigate and be defined, apply 1 cleaning up excrement water every two weeks within the 2nd year, apply cleaning up excrement water and be defined so that tree root is irrigated, monthly apply 1 composite fertilizer, every plant
Apply 200g, ring is applied, and is timely loosened the soil and weeding, is focused on draining, is prevented treelet from being soaked by ponding, cause treelet by high temperature injury or
It is dead；
4) Harmful is prevented and treated：Rhizome maize ear rot and rotten, sprinkling on time easily occur at rainy season for Chinese yew
70% 500-800 times of fenaminosulf liquid is on the defensive and treated；And sick, red tangerine disease of leaf tangerine etc. easily occurs in dry season, with 1% ripple
You are more, and liquid carries out sprinkling prevention；For the phenomenon of locust food leaf, 500-800 times of liquid defence of metrifonate is timely sprayed；For snail
The harm of ox, trapped to tag and feed with snail jinx and killed, prohibit the use of DDT etc. that there is high residue, carcinogenic, hypertoxic, slow poison
Agricultural chemicals carry out the prevention and control of plant diseases, pest control；
Nutrient solution described in step 3), the method for being prepared by the following procedure obtain：
1. Feedstock treating：The fresh complete stool of Chinese yew or fresh branches and leaves are cleaned up, dries, is crushed with pulverizer, with 10mm nets
Hole pulverizer crushes, grinding particle size 2-10mm；
2. extract：By step 1. obtain crush raw material add 2 times of weight methanol heating extraction, 45 DEG C extract 3 times, often
Secondary 2h, filtering merge concentrate, concentration, temperature control≤50 DEG C, and vacuum control is concentrated into 0.95- in -0.08 ± 0.01MPa
1.05g/ml, concentrated extracting solution is obtained, detect content of taxol；
3. aqueous two-phase extraction：Medicinal extract is 2. obtained to step and carries out aqueous two-phase extraction, medicinal extract and aqueous two-phase extraction liquid are according to 1:
1.25 volume ratio, the upper phase of extraction and the lower phase of extraction are obtained, double-aqueous phase system is by polyethylene glycol, chlorine used in aqueous two-phase extraction
Change sodium and water is formed, mass percent of the polyethylene glycol in the double-aqueous phase system is 15%, and sodium chloride is in the aqueous two-phase
Mass percent in system is 15%, and surplus is water, and the weight average molecular weight of the polyethylene glycol is 8000；
4. 3. aqueous phase extracted that step is obtained, milipore filter desalination of the molecular cut off for 1000-100000 is used, is obtained
Arrive, 80-100 times of dilution uses.
Comparative example：
The same embodiment of implantation methods, not using nutrient solution.
Experimental example：
The detection of taxol：The fresh branches and leaves of Chinese yew are cleaned up, dries, is crushed with pulverizer, are crushed with 10mm mesh
Machine crushes, grinding particle size 2-10mm, detects content of taxol.Appropriate amount of sample is taken, it is accurately weighed, dissolved with methanol (chromatographically pure)
In 10ml or 25ml volumetric flasks, it is completely dissolved in ultrasonic echography 3-5min, is settled to scale.Take the sample after a certain amount of dissolving
Product, 5min is centrifuged in supercentrifuge 10000rpm.Take the supernatant after centrifugation appropriate, filtered with syringe by 0.45 μm of filter membrane
Cross, inject in sample bottle, it is to be detected.Detected sample is measured using high performance liquid chromatograph.Testing conditions：Sample size
For 5 μ l, C18 posts (250X4.6mm), 25 DEG C of column temperature, acetonitrile-water (34：69) isocratic elution 10min, then with per minute 1.75%
Speed improve acetonitrile ratio to acetonitrile-water (45：55) the complete appearance of target substance, is eluted to, obtains peak area.Utilize external standard
Method is quantified, and based on dry product, content of taxol cannot be less than 0.3%.
Place：Guilin Xingan mountain area, height above sea level 800-1000m
The 6-7 months then are colonized, and Second Year takes fresh branches and leaves to detect the 11-12 months.
Claims (5)
1. a kind of implantation methods of Chinese yew, it is characterised in that comprise the following steps：
1) planting environment：Select Guilin height above sea level 600-1000m, landform is gentle, the gradient be less than 35 °, soil layer it is loose, it is deep,
Draining is convenient, moistens, soil or mountain region rich in organic matter and acid soil；
2) it is colonized, afforests：In June to December, cleaning site preparation is carried out to forest land in a manner of banding, ploughs deeply and carefully ploughs, be made into small sections of a vegetable plot
Shape, while apply the base manure of abundance, it is mixed even with ploughing deeply, plants Chinese yew seedling, nursery stock can not tilt, root system forward horizontal exhibition
Open, it is native in covering, avoid nest root, then covering some on the face of cave loosens the soil, allow its it is micro- be above the ground level, prevent rainy season ponding；
3) tending management：If finding that dead strain is filled in time after being colonized 1 week, field planting applies weekly 1 nutrient solution on the 1st year after surviving, with
Tree root is irrigated and is defined, timing is applied fertilizer, and is timely loosened the soil and weeding, is focused on draining, is prevented treelet from being soaked by ponding, cause treelet
By high temperature injury or death；
4) Harmful is prevented and treated；
Nutrient solution described in step 3), the method for being prepared by the following procedure obtain：
1. Feedstock treating：The fresh complete stool of Chinese yew or fresh branches and leaves are cleaned up, dries, is crushed with pulverizer, with 10mm mesh powder
Broken machine crushes, grinding particle size 2-10mm；
2. extract：By step 1. obtain crush raw material add 2 times of weight methanol heating extraction, 45 DEG C extract 3 times, every time
2h, filtering merge concentrate, concentration, temperature control≤50 DEG C, and vacuum control is concentrated into 0.95- in -0.08 ± 0.01MPa
1.05g/ml, concentrated extracting solution is obtained, detect content of taxol；
3. aqueous two-phase extraction：Medicinal extract is 2. obtained to step and carries out aqueous two-phase extraction, medicinal extract and aqueous two-phase extraction liquid are according to 1:1.25
Volume ratio, obtain that extraction is upper mutually and the lower phase of extraction, and double-aqueous phase system is by polyethylene glycol, sodium chloride used in aqueous two-phase extraction
Formed with water, mass percent of the polyethylene glycol in the double-aqueous phase system is 15%, and sodium chloride is in the double-aqueous phase system
In mass percent be 15%, surplus is water, and the weight average molecular weight of the polyethylene glycol is 8000；
4. 3. aqueous phase extracted that step is obtained, milipore filter desalination of the molecular cut off for 1000-100000 is used, is obtained.
A kind of 2. implantation methods of Chinese yew according to claim 1, it is characterised in that：Chinese yew kind described in step 2)
Seedling, be 2.5 years raw taxusyunnanensis cuttage seedings of artificial culture, ground diameter more than 0.3cm, height of seedling more than 50cm one-level
Seedling, or the nutrition that 1.5 years raw seedlings of southerm yew of artificial culture, average height of seedling 25cm, average ground diameter are 0.08cm
Bag seedling.
A kind of 3. implantation methods of Chinese yew according to claim 1, it is characterised in that：Small sections of a vegetable plot are made into described in step 2)
Shape, specification 40cmx40cmx30cm, described plantation, plant distance is 0.4mx1.5m, 1.65 ten thousand plants/hm of density2。
A kind of 4. implantation methods of Chinese yew according to claim 1, it is characterised in that：Timing described in step 3) is applied
Fertilizer, refer to that field planting applies weekly 1 cleaning up excrement water on the 1st year after surviving, apply cleaning up excrement water and be defined so that tree root is irrigated, apply 1 every two weeks within the 2nd year
Secondary cleaning up excrement water, apply cleaning up excrement water and be defined so that tree root is irrigated, monthly apply 1 composite fertilizer, every plant is applied 200g, and ring is applied.
A kind of 5. implantation methods of Chinese yew according to claim 1, it is characterised in that：Nutrient solution described in step 3),
80-100 times of dilution uses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710703317.5A CN107360932A (en) | 2017-08-16 | 2017-08-16 | A kind of implantation methods of Chinese yew |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710703317.5A CN107360932A (en) | 2017-08-16 | 2017-08-16 | A kind of implantation methods of Chinese yew |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107360932A true CN107360932A (en) | 2017-11-21 |
Family
ID=60309641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710703317.5A Pending CN107360932A (en) | 2017-08-16 | 2017-08-16 | A kind of implantation methods of Chinese yew |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107360932A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109906858A (en) * | 2019-04-12 | 2019-06-21 | 义乌博锐信息科技有限公司 | A kind of breeding method of Chinese yew |
| CN112703952A (en) * | 2020-12-24 | 2021-04-27 | 黑龙江省林业科学院伊春分院 | Forestation method using salix integra nutrition pot seedlings |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498853A (en) * | 2011-10-21 | 2012-06-20 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN103299820A (en) * | 2013-07-08 | 2013-09-18 | 中国林业科学研究院资源昆虫研究所 | Culturing method for improving content of paclitaxel in taxus chinensis |
| CN104365426A (en) * | 2014-11-21 | 2015-02-25 | 苏州市吴中区光福香雪苗圃 | Planting method for Chinese yew |
| CN106718487A (en) * | 2016-11-16 | 2017-05-31 | 普定县科技服务中心 | A kind of method for culturing seedlings of Chinese yew |
-
2017
- 2017-08-16 CN CN201710703317.5A patent/CN107360932A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498853A (en) * | 2011-10-21 | 2012-06-20 | 江苏红豆杉生物科技有限公司 | Planting and harvesting method for improving 10-DABIII content of artificially planted laxus malrel |
| CN103299820A (en) * | 2013-07-08 | 2013-09-18 | 中国林业科学研究院资源昆虫研究所 | Culturing method for improving content of paclitaxel in taxus chinensis |
| CN104365426A (en) * | 2014-11-21 | 2015-02-25 | 苏州市吴中区光福香雪苗圃 | Planting method for Chinese yew |
| CN106718487A (en) * | 2016-11-16 | 2017-05-31 | 普定县科技服务中心 | A kind of method for culturing seedlings of Chinese yew |
Non-Patent Citations (2)
| Title |
|---|
| 刘杨等: "成盐无机盐种类对PEG双水相相平衡的影响", 《化学研究与应用》 * |
| 戴柳林: "南方红豆杉的特性及其种植技术探究", 《民营科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109906858A (en) * | 2019-04-12 | 2019-06-21 | 义乌博锐信息科技有限公司 | A kind of breeding method of Chinese yew |
| CN112703952A (en) * | 2020-12-24 | 2021-04-27 | 黑龙江省林业科学院伊春分院 | Forestation method using salix integra nutrition pot seedlings |
| CN112703952B (en) * | 2020-12-24 | 2023-12-12 | 黑龙江省林业科学院伊春分院 | Forestation method using salix tenuifolia nutrition pot seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104094750B (en) | Pollution-free food cabbage mustard cultivation method | |
| CN104221673B (en) | The cultivation method of Camellia nitidissima under a kind of Eucalyptus Stand | |
| JP2010029181A (en) | Method for cultivating medicinal herb | |
| CN107295938A (en) | The implantation methods of Chinese yew | |
| Sun et al. | A novel silvicultural model for increasing biopolymer production from Eucommia ulmoides Oliver trees | |
| CN105580701A (en) | Method for planting tea trees | |
| CN105104129A (en) | Dendrobium cultivation matrix and preparation method thereof | |
| CN104041299A (en) | Two-step seedling culturing method for larches | |
| CN104255353A (en) | Organic covering material for cultivating Chinese yams, method for manufacturing organic covering material and application thereof | |
| CN102860194B (en) | Planting method of taxus chinensis and application of 5-aminolevulinic acid | |
| CN109392598A (en) | A kind of Wildmimic cultivation method of ganoderma lucidum | |
| Ehsanullah et al. | Effect of pinching on growth and quality flower production of chrysanthemum (Chrysanthemum indicum L.) | |
| CN107667809A (en) | A kind of high yield cultivating method of selenium-rich beautiful millettia root | |
| CN102792831B (en) | High-efficiency rapid propagation technique for Chinese yew | |
| CN107360932A (en) | A kind of implantation methods of Chinese yew | |
| CN109997583A (en) | A method of it is grown using rice straw prevention and treatment fruit tree farmland weed | |
| CN102523862A (en) | Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection | |
| CN104855199A (en) | Method for planting rose apple | |
| JP5282199B2 (en) | Hatake-shimeji culture medium and cultivation method thereof | |
| CN102960145A (en) | Eco-economical revegetation method for carrying out water and soil conservation in karst dry-hot valley by utilizing Phyllanthus emblca L. | |
| CN107278580A (en) | A kind of underwood planting method of roxburgh anoectochilus terminal bud | |
| CN104126380A (en) | Sarcandra glabra cultivation method | |
| CN109169120B (en) | Allelopathy-based leguminous arbor and grass composite planting method and verification method thereof | |
| CN108496665A (en) | A kind of Illicium verum Forest is sowed the method for grass planting coral | |
| Antonov et al. | Application of a vermicomposter containing biostimulant for pine tapping |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171121 |
|
| WD01 | Invention patent application deemed withdrawn after publication |