CN102172136A - Method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi - Google Patents
Method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi Download PDFInfo
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Abstract
The invention relates to a method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi. The method comprises the steps of: selecting populus diversifolia sprouts for performing pot experiment, arranging a transplanting hole in the pot, spreading sandy soils rich in glomus mosseae spores at the bottom of the hole, slowly rotating the root systems of populus diversifolia seedlings on the sandy soils of glomus mosseae spores before the transplantation, so that the sandy soils are pasted on the root systems of the populus diversifolia seedlings, transplanting the populus diversifolia seedlings into the hole in the pot, watering a dilute Hoagland nutrient solution every time, and observing the survival rate; or enabling populus diversifolia seeds to absorb water and swell and accelerating germination in an incubator, mixing the populus diversifolia seeds with the sandy soils of glomus mosseae spores, spreading the mixture on a sandy layer surface of a culture bed, and paving a layer of sandy soils rich in glomus mosseae spores under the sandy layer of the culture bed in advance, wherein the root systems are to pass through the sandy soil layer rich in glomus mosseae spores, so that the root systems can be infected. Compared with a control group, the populus diversifolia seedlings obtained by the method are obviously different from the populus diversifolia seedlings in the control group in the growing speed and survival time, particularly in survival rate.
Description
Technical field
The present invention relates to a kind of method that arbuscular mycorrhizal fungi improves diversiform-leaved poplar artificial breeding survival rate of inoculating.
Background technology
Diversiform-leaved poplar (Populus euphratica) is the tall and big deciduous tree of Salicaceae, rare shrub, is distinctive ancient, the rare tree species of Desert Area.Natural distribution in north African, the Middle East and the riverbank of NORTHWEST CHINA arid biogeographic zone and the salt lick of low humidity, be the important arbor species in NW China desert and half-desert area, at present, only preserve the large tracts of land forest land in the desert district of China, concentrate to be distributed in the Tarim Basin.The drought resisting that the diversiform-leaved poplar tool is very strong, salt tolerant alkali, windproof, ability such as fix the sand, be the important degeneration-resistant germ plasm resource in Desert Oasis area.
In China western part large-area arid area is arranged, cause land capability to descend because human unreasonable activity in production and fragile ecological environment interact, the land resources forfeiture, the threat of desertification, desertization is serious day by day.Some have the plant quantity minimizing of important function or are on the verge of impasse in the ecosystem.
Introducing a fine variety of plant is the ecology recovering systemic-function, desertification, desertization control; The important means of bio-diversity conservation and urban afforestation.The transplanting of plant also is even more important in agriculture forest and husbandry production, medicinal plant exploitation.Creating favourable habitat for plant introducing a fine variety of plant in the migration process is successful key, under the guidance of " weather theory of similarity ", " ecological historical analysis method ", " ecological factor opinion " and " composite factor opinion ", crown density, soil pH value, soil N, P, K content, the significant effects factors such as water capacity, frost-free season, soil depth, rainfall and average temperature of the whole year are to introduce a fine variety consideration more in the migration process (Li Guoqing, Liu Jun intelligent 1984).Yet action of microorganisms is toward be left in the basket habitually in the past.
Mycorhiza (Mycorrhizae) is a kind of general plant symbiosis phenomenon of natural world, be a kind of association that mycorrhizal fungi mycelia in the soil and higher plant nutrition root system form, mycorrhizal fungi is one of biomass maximum, most important member in the soil microflora.And the fungi that can form " clump branch " shape structure in the plant roots cell is called as: and arbuscular mycorrhizal fungi (Arbuscular Mycorrhizal Fungi, both: the AM fungi).Arbuscular mycorrhiza then be distribute the widest, with agricultural herd the closest class mycorhiza of relation, the vascular plant on the earth more than 90% can both form arbuscular mycorrhiza.
The AM fungi is constantly disclosed for plant growing importance, function in arid area ecology recovering system, depend on these system's parts are understood more completely, studies show that in the relation of the reconstruction of arid area vegetation and AM fungi very closely, all contact produces positive response to not every plant to mycorhiza.Some plants must rely on mycorhiza community could survive or finish its life cycle; Other plants are had benefited from mycorhiza under certain condition, are facultative communities; And have some plants output and survival rate after to descend by the AM fungal infection.The scope of plant roots and fungi community make a profit what and time and AM fungal colonization is relevant, and the functional evidence of getting in touch that may exist between advantitious plant and the AM fungi might find by the pattern that detects itself and root AM fungal colonization; The ecological characteristic of habitat feature (soil physical chemistry the hydrology weather etc.) or AM fungi may determine the dynamic change of AM fungi, and the difference in plant growing ground feature and plant site may cause the difference that AM classification of fungi group is very big, thereby can influence growth and development of plant, because different AM classification of fungi groups offers the nutrient of plant and promotes the ability of plant growing that very big difference is also arranged at them, especially very strong to mycorrhizal dependency plant, the plant fast growing period is relevant with the high level of fungal colonization in general, also relevant with the kind of fungi, some kinds of the mould door of sacculus (Glomeromycota) can promote the expansion of root system of plant to extend, and improve the picked-up of nutrition and the growth of promotion plant.People such as van der Heijden in 1998 the AM fungi effect therein in the 11 plant species symbiosis processes that begins one's study nearly, studying different AM classification of fungi groups influences the range size of nutrient distribution between aulophyte, and different AM classification of fungi groups to what extent change the ability of plant symbiosis relation, more quantized to find in the experiment afterwards, the have the ability symbiotic relation of regulating plant of AM fungi by the distribution of nutrition between aulophyte, the symbiosis ability of its competitive ability of nutritional amt decision that a plant species is obtained and it and other plant.The AM fungal species is rich forms the words that have similitude in a plant species in different habitats with group, the structure of plant community may be subjected to the influence of AM fungal species so, conversely, aulophyte may also can influence the structure of AM fungal colonization, but also might plant and the AM fungal species show that a kind of interdepending adapts to the relation of these habitat soil and weather conditions.
The effect that produces on aulophyte may and change along with AM fungal species composition, the AM fungi also is incoordinate to the effect of plant, change or the local AM fungal colonization that changes an ecological system will have influence on ensuing process, reduce because of agriculture behavior in semiarid Mediterranean Region AM fungal population quantity, after stopping 45 years, agro-farming just recovers, the suggestion mycorhiza is very important for the establiss of plant in natural ecosystems and survival, and it is also extremely important to emphasize to measure the kind of an indigenous mycorhiza in the ecosystem.
Therefore, in the Arid land ecosystems, the AM fungi is playing the part of important role, and it not only might determine the kind of plant, the distribution of nutritional resource with other biology and abiotic factor, even might influence structure of plant community and diversity.In the process of introducing a fine variety to being familiar with to some extent with kind, quantity and the ecological characteristic thereof of the symbiosis AM fungi of plant, to not only help so to introduce a fine variety and transplant plant survival, growth, population child care, and can provide theoretical foundation for the recovery of ecosystem function, so that implement more effective recovery measure; Introducing a fine variety of plant artificially inoculated the AM fungi in the process in arid area agriculture forest and husbandry production, medicinal plant exploitation, will promote growth and development of plant, strengthens plant resistance to environment stress, improve crop yield and quality.
By 2007-2010 to the arid area, Xinjiang common build the mass-planting thing: check winds and fix drifting sand, investigate, understand quantity, kind and the level of AM fungal colonization with its Rhizosphere AM Fungi diversity of diversiform-leaved poplar (Populus euphratica) of material arbor; Main bacterial strain is cultivated and preservation; The diversiform-leaved poplar seedling is carried out the inoculation of AM fungi before transplanting, measure its survival rate, measure the situation of growing.
The diversiform-leaved poplar standing forest in Xinjiang Tarim River middle reaches and downstream, Laolong river is not gathered 13 soil and root system sample, soil sample by wet sieving from, identify AM fungi 3 at the diversiform-leaved poplar root region soil and belong to 15 kinds: thin recessed nothing stalk capsule mould (A.scrobiculata), be stained with bits various sporangiocyst mould (D.spurcum), raspberry shape sacculus mould (G.rubiforme), the sacculus that contracts mould (G.constrictum), big fruit sacculus mould (G.macrocarpum), sandy wasteland sacculus mould (G.deserticola), the printing opacity sacculus is mould, (G.diaphanum), ground sacculus mould (G.geosporum), He Shi sacculus mould (G.hoi), cassava sacculus mould (G.manihotis), children's cover sacculus mould (G.etunicatum), ivory white sacculus mould (G.eburneum), Glomus mosseae (G..mosseae), stratiform sacculus mould (G.lamellosum), clumping sacculus mould (G.aggregatum).(Wang Youshan, Chen Li 2010)
The root sample is measured by dyeing and is shown that diversiform-leaved poplar can be set up good symbiont with the AM fungi, and the diversiform-leaved poplar root system infects frequency and infection strength can reach 80% and 100% respectively.
Relevant with arbuscular mycorrhiza in recent years research has become focus, and through retrieval, the arbuscular mycorrhiza application study mainly concentrates on economic crops, cereal crops and fruit tree.A small amount of inoculation study to ornamental plants is also arranged.Therefore, arbuscular mycorrhiza is used and is still had very big development space.The application of arbuscular mycorrhizal fungi, frequency of utilization are Paraglomus Glomus according to this, the mould genus of huge sporangiocyst Gigaspora, acaulospora specie Acaulospora, the mould genus of shield macrocyst Scutellospora, the hard mould genus of capsule Sclerocystis.In the big tree transplanting process of city, emphasize as far as possible primitive root district soil that keep more, be able to more reservation in order to make plant rhizosphere microbe.
Various countries drop into a large amount of human and material resources aspect the AM fungal studies, except strengthening that basic research such as mycorhiza classification, physiological ecological are still the focus.Have at mycorhiza application facet emphasis: 1. the seed selection of strain excellent and appraisement system are set up.Cultivate by conventional and nconventional method and to accommodate the multiple ecosystem, high degeneration-resistant, and the mycorrhizal fungi of industrial severe contamination area application.And set up AM fungal gene storehouse.2. AM fungi--the condition research of-plant mutualistic symbiosis.The regulation and control factor that research is benefited plant to the full extent, as the research of the best inoculation period and dosage of inoculation and ecological condition and tillage method to the growth of mycorhiza and the influence of effect.3. Mycorrhizal cultivating seedlings system and mycorhiza bacterium promote and have the application of commercial value in the large tracts of land in field.Pay the utmost attention to application study at some preciousnesses, rare seeds, desert reproducting tree species and fruit tree, flowers, medicinal plant etc.4. the AM bacterium is to action effect, the mechanism of action of plant, and the AM fungi influences each other and acts on other edaphons and soil are zootic.At present, the research to the AM bacterium in the world wide is in a kind of extremely active state, and to its ecology, physiology, all there are a large amount of reports aspects such as The application of new technique.Set up the commercial-scale tree nursery of transplanting seeds inoculation AM fungi as University of Hawaii (Miyasaka, 2001), and sapling behind the inoculation AM fungi is transplanted in the other places, and prove that the inoculation of AM fungi is favourable.Can predict, although the AM fungi the difficulty of separation, cultivation and evaluation may influence to a certain extent and apply, AM fungi application technology will can be moved towards commercialization gradually.Become an indispensable biotechnology in the agricultural production from now on.
The AM fungi report relevant with the arid area, Xinjiang has classification and the ecological reports that distribute such as Zhang Meiqing the beginning of the nineties, in recent years report concentrates on regional bio-diversity and distribution to the north of the Kuerle, and the research aspect AM fungi and husky the livings ephemeral ecology is arranged in the accurate Ge Er basin in the north, Xinjiang.
Effect to AM fungi in the arid biogeographic zone ecosystem receives much attention in recent years, Allen etc. think that the effect of arid biogeographic zone mycorhiza mainly shows as drought resistance that increases plant and the transfer rate that improves moisture, Benthlenfalvay etc. think that then mycorhiza can change soil structure and improve water capacity, and domestic have
Liu Runjin,
Li XiaolinIn the influence research of endogenous hormones to plant drought, there is the AM fungi to produce some chemical substances abroad in recent years, as water conservation albumen the plant drought ability is increased.
Diversiform-leaved poplar is mainly built the mass-planting thing as the arid area, and domestic relevant research to the diversiform-leaved poplar child care at present mainly concentrates on the plant physiology aspect of abiotic factor such as water, salt and diversiform-leaved poplar, and still nobody relates to the research of AM mycosymbiosis relation.
Camel thorn has the report of a small amount of taxonomy and the research of plant physiology aspect, there is no report both at home and abroad with the research of AM mycosymbiosis.
Summary of the invention
The objective of the invention is to, a kind of method that arbuscular mycorrhizal fungi improves diversiform-leaved poplar artificial breeding survival rate of inoculating is provided, this method is by selecting the diversiform-leaved poplar seedling to carry out pot experiment, sandy soil in the pot experiment are sterilized according to a conventional method, in basin, establish then and transplant the hole, be rich in the sandy soil of Glomus mosseae spore at bottom, hole spreading, on the sandy soil of Glomus mosseae spore, slowly rotate before more moistening diversiform-leaved poplar seedlings root being transplanted, make the diversiform-leaved poplar seedlings root be stained with sandy soil, be transplanted in the basin in the hole, soil pressure on every side is real, water dilution back He Gelunte nutrient solution then at every turn, observe survival rate and get final product; Or with diversiform-leaved poplar seed imbibition vernalization sprouting in incubator, mix with the sandy soil of Glomus mosseae spore, spreading is on preprepared culture bed sand bed surface, overlay one deck below the culture bed sand bed and be rich in the sandy soil of Glomus mosseae spore, root system will can be infected by being rich in the sandy soil layer of Glomus mosseae spore.Compare with control group by this method, there were significant differences with control group diversiform-leaved poplar seedling in growth rate with on the time-to-live for the diversiform-leaved poplar seedling that the method for the invention obtains, and particularly survival rate has notable difference.
A kind of method that arbuscular mycorrhizal fungi improves diversiform-leaved poplar artificial breeding survival rate of inoculating of the present invention follows these steps to carry out:
A, selection diversiform-leaved poplar seedling or diversiform-leaved poplar seed, diversiform-leaved poplar seedling plant height 15-20cm, number of blade 22-35 sheet, plant fresh weight 2.3-2.6g carries out pot experiment, transplants the April 30 in spring;
B, the sandy soil in the pot experiment are sterilized according to a conventional method, and placed 15-20 days, it is dark to establish 10-12cm then in basin, diameter is the transplanting hole of 5cm, be rich in the sandy soil of Glomus mosseae spore at bottom, hole spreading, slowly rotate on the sandy soil of Glomus mosseae spore before again that step a is moistening diversiform-leaved poplar seedlings root is transplanted, make the diversiform-leaved poplar seedlings root be stained with sandy soil, that guarantees that root system is subjected to infects;
C, step b is stained with sandy soil again the diversiform-leaved poplar seedling replanting in the basin in the hole, with around soil pressure real, water dilution back He Gelunte nutrient solution then at every turn, observe survival rate and get final product;
D or with step a diversiform-leaved poplar seed imbibition 8-12 hour, vernalization is sprouted in incubator, and temperature 25-28 ℃, relative moisture 30-50% cultivates after 3-5 days and mixes with the sandy soil of Glomus mosseae spore, and spreading is on preprepared culture bed sand bed surface;
E, below the culture bed sand bed 2-3cm of steps d, overlay the sandy soil that one deck 2-3mm is rich in the Glomus mosseae spore, temperature is controlled at 20-25 ℃, will sprout in 5-10 days, sprouts the back 10-15 days, root system will can be infected by being rich in the sandy soil layer of Glomus mosseae spore.
Step b is 2-3mm at the sandy soil that bottom, hole spreading is rich in the Glomus mosseae spore.
The sandy soil of step b and steps d Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand.
Overlaying the sandy soil that one deck is rich in the Glomus mosseae spore below the culture bed sand bed 2-3cm of step e is 2-3mm, and the gross thickness of culture bed sand bed is 20-50cm.
A kind of method that arbuscular mycorrhizal fungi improves diversiform-leaved poplar artificial breeding survival rate of inoculating of the present invention, pot experiment is provided by desert plant garden, Chinese Academy of Sciences Turfan for selecting diversiform-leaved poplar (Populus euphratica) seedling in this method, in 10 months seedling of hundreds of strains, select 48 strains, potted plantly be divided into two groups, every group 24 strain, one group is contrast, one winding kind, plant height does not have significant difference (15-20CM), number of blade difference does not have significant difference (22-35 sheet), and the plant fresh weight does not have significant difference (2.3-2.6G);
Blastocyst door Glomeromycota, sacculus Gammaproteobacteria Glomeromycete, the mould order Glomerale of sacculus, the sacculus Glomeraceae. of mould section Paraglomus Glomus, Glomus mosseae G.mosseae (separate from the Xinjiang Luntai);
Potted plant sandy soil are the sterilization sandy soil and placed 15-20 days;
The sandy soil of Glomus mosseae spore: be the washed-out sand less than 2mm, 15-25 spore/ml sand expands numerous by Chinese sorghum.
The contrast of at every turn watering is all watered with He Gelunte (Hoagland) nutrient solution of concentration with the dilution of volume with processing, the illumination in according to a conventional method, and temperature, two groups of environmental factors such as humidity are all consistent.
The preparation of nutrient solution: the various countries scientist successively develops hundreds of nutrient solution prescriptions, and wherein, He Gelunte (Hoagland) nutrient solution is a kind of application nutrient solution more widely.
The prescription of He Gelunte nutrient solution is:
The milliliter number that adds in every liter of culture fluid of macroelement is KH
2PO
4Concentration is 1mol, 1ml; KNO
3Concentration is 1mol, 5ml; Ca (NO
3)
2Concentration is 1mol, 5ml; MgSO
4Concentration is 1mol, 2ml;
The gram number that adds in every liter of culture fluid of trace element is H
3BO
32.86g, MnCl
24H
2O1.81g, ZnSO
47H
2O 0.22g, CuSO
45H
2O 0.08g, H
2MoO4H
2O 0.02g;
Add 1mL Fe EDTA solution in every liter of culture fluid, then with the macroelement that adds, trace element and Fe EDTA solution mix and get final product.
There were significant differences with non-inoculation diversiform-leaved poplar seedling in growth rate with on the time-to-live by inoculation diversiform-leaved poplar seedling, and particularly survival rate has notable difference.
Diversiform-leaved poplar seed inoculation method: in incubator, diversiform-leaved poplar seed imbibition 8-12 hour, 25-28 ℃ of temperature, 30-50% relative moisture is cultivated vernalization in 3-5 days, seed mixes the back spreading on the good culture bed sand bed surface of pre-preparation after the vernalization with sandy soil, 2-3cm overlays the sandy soil that one deck 2-3mm is rich in Glomus mosseae G.mosseae spore under this culture bed sand bed, temperature is controlled at 20-25 ℃, to sprout in 5-10 days, sprouted the back 10-15 days, root system will be by being rich in the sandy soil layer of Glomus mosseae G.mosseae spore, thereby be subjected to infecting of AM fungi.Handle and need not inoculation again when transplanting.
Because in the artificial breeding process, no matter be incubator suction vernalization, still sprout with sandy soil mixing seedbed, or the transplanting process, all different with natural surroundings, seedling is descended by the possibility of useful AM fungal infection, the present invention includes two stages of stage of taking root and transplanting stage of sprouting from the seedbed can carry out the inoculation of AM fungi orientation, make it be subjected to infecting of useful AM fungi, improve its survival rate, and the condition of necessity is provided for healthy growth thereafter.
Diversiform-leaved poplar inoculation Glomus mosseae G.mosseae grows and survives information slip
As can be seen from the table: there were significant differences with non-diversiform-leaved poplar seedling in growth rate with on the time-to-live to be inoculated the diversiform-leaved poplar seedling, and particularly survival rate has notable difference.
Embodiment
Embodiment 1
Select the diversiform-leaved poplar seedling, diversiform-leaved poplar seedling plant height 15cm, 22 of the numbers of blade, plant fresh weight 2.3g carries out pot experiment, transplants the mid-April in spring;
Sandy soil in the pot experiment are sterilized according to a conventional method, and placed 15 days, it is dark to establish 10cm then in basin, diameter is the transplanting hole of 5cm, and spreading is rich in the sandy soil 2mm of Glomus mosseae spore bottom the hole, slowly rotates on the sandy soil of Glomus mosseae spore before diversiform-leaved poplar seedlings root that will be moistening is transplanted again, make the diversiform-leaved poplar seedlings root be stained with sandy soil, the sandy soil of Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand, and that guarantees that root system is subjected to infects;
The diversiform-leaved poplar seedling replanting that will be stained with sandy soil again in the basin in the hole, with around soil pressure real, water the He Gelunte nutrient solution after the dilution then at every turn, observe survival rate and get final product;
Embodiment 2
Select diversiform-leaved poplar seedling or diversiform-leaved poplar seed, diversiform-leaved poplar seedling plant height 18cm, 30 of the numbers of blade, plant fresh weight 2.5g carries out pot experiment, in the transplanting by the end of April in spring;
Sandy soil in the pot experiment are sterilized according to a conventional method, and put 18 days, it is dark to establish 11cm then in basin, diameter is the transplanting hole of 5cm, and spreading is rich in the sandy soil 3mm of Glomus mosseae spore bottom the hole, slowly rotates on the sandy soil of Glomus mosseae spore before again that step a is moistening diversiform-leaved poplar seedlings root is transplanted, make the diversiform-leaved poplar seedlings root be stained with sandy soil, the sandy soil of Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand, and that guarantees that root system is subjected to infects;
The diversiform-leaved poplar seedling replanting that will be stained with sandy soil again in the basin in the hole, with around soil pressure real, water dilution back He Gelunte nutrient solution then at every turn, observe survival rate and get final product;
Embodiment 3
Select the diversiform-leaved poplar seedling, diversiform-leaved poplar seedling plant height 20cm, 35 of the numbers of blade, plant fresh weight 2.6g carries out pot experiment, in the transplanting by the end of April in spring;
Sandy soil in the pot experiment are sterilized according to a conventional method, and put 20 days, it is dark to establish 12cm then in basin, diameter is the transplanting hole of 5cm, and spreading is rich in the sandy soil 3mm of Glomus mosseae spore bottom the hole, slowly rotates on the sandy soil of Glomus mosseae spore before again that step a is moistening diversiform-leaved poplar seedlings root is transplanted, make the diversiform-leaved poplar seedlings root be stained with sandy soil, the sandy soil of Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand, and that guarantees that root system is subjected to infects;
The diversiform-leaved poplar seedling replanting that will be stained with sandy soil again in the basin in the hole, with around soil pressure real, water dilution back He Gelunte nutrient solution then at every turn, observe survival rate and get final product.
Embodiment 4
Select the diversiform-leaved poplar seed of collection last year, begin the work of growing seedlings by the end of June, imbibition 12 hours, vernalization is sprouted in incubator, 25 ℃ of temperature, relative moisture 30% is cultivated after 3 days and is mixed with the sandy soil of Glomus mosseae spore, the sandy soil of Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand, seed and inoculation sandy soil volume ratio 1: 3,2500/ square metre of seed density, spreading is on preprepared culture bed sand bed surface, and wherein culture bed sand bed thickness is 30cm;
2-3cm below the culture bed sand bed is overlay the sandy soil that one deck 2mm is rich in the Glomus mosseae spore, temperature is controlled at 20 ℃, to sprout in 5 days, sprout back 15 days, root system will be by being rich in the sandy soil layer of Glomus mosseae spore, can be infected, treat seedling plant height 3-5cm after 40 days, transplant when true leaf is counted the 5-6 sheet, transplant line-spacing 20cm, spacing in the rows 5cm can observe its growth and survive situation after next year.
Embodiment 5
Select the diversiform-leaved poplar seed of collection then, begin the work of growing seedlings at the beginning of 7 months, imbibition 9 hours, vernalization is sprouted in incubator, 26 ℃ of temperature, relative moisture 40% is cultivated after 4 days and is mixed with the sandy soil of Glomus mosseae spore, spreading is on preprepared culture bed sand bed surface, the sandy soil of Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand, seed and inoculation sandy soil volume ratio 1: 3,2700/ square metre of seed density, wherein culture bed sand bed thickness is 25cm;
2-3cm below the culture bed sand bed is overlay the sandy soil that one deck 3mm is rich in the Glomus mosseae spore, temperature is controlled at 23 ℃, to sprout in 8 days, sprout back 12 days, root system will be by being rich in the sandy soil layer of Glomus mosseae spore, can be infected, treat seedling plant height 3-5cm after 35 days, transplant when true leaf is counted the 5-6 sheet, transplant line-spacing 10cm, spacing in the rows 10cm can observe its growth and survive situation after next year.
Embodiment 6
Select the diversiform-leaved poplar seed of collection then, imbibition 10 hours, 28 ℃ of temperature are sprouted in vernalization in incubator, relative moisture 50%, cultivate after 5 days and mix with the sandy soil of Glomus mosseae spore, spreading is on preprepared culture bed sand bed surface, and the sandy soil of Glomus mosseae spore are the washed-out sand of particle diameter less than 2mm, 15-25 spore/ml sand, seed and inoculation sandy soil volume ratio 1: 3,3000/ square metre of seed density, wherein culture bed sand bed thickness is 20cm;
2-3cm below the culture bed sand bed is overlay the sandy soil that one deck 3mm is rich in the Glomus mosseae spore, temperature is controlled at 25 ℃, to sprout in 10 days, sprouted back 10 days, root system will can be subjected to infecting and treat seedling plant height 3-5cm after 35 days by being rich in the sandy soil layer of Glomus mosseae spore, when counting the 5-6 sheet, true leaf transplants, transplant line-spacing 15cm, spacing in the rows 15cm can observe its growth and survive situation after next year.
Claims (4)
1. inoculate the method that arbuscular mycorrhizal fungi improves diversiform-leaved poplar artificial breeding survival rate for one kind, it is characterized in that following these steps to carrying out:
A, selection diversiform-leaved poplar seedling or diversiform-leaved poplar seed, diversiform-leaved poplar seedling plant height 15-20cm, number of blade 22-35 sheet, plant fresh weight 2.3-2.6g carries out pot experiment, transplants the April 30 in spring;
B, the sandy soil in the pot experiment are sterilized according to a conventional method, and placed 15-20 days, it is dark to establish 10-12cm then in basin, diameter is the transplanting hole of 5cm, be rich in the sandy soil of Glomus mosseae spore at bottom, hole spreading, slowly rotate on the sandy soil of Glomus mosseae spore before again that step a is moistening diversiform-leaved poplar seedlings root is transplanted, make the diversiform-leaved poplar seedlings root be stained with sandy soil, that guarantees that root system is subjected to infects;
C, step b is stained with sandy soil again the diversiform-leaved poplar seedling replanting in the basin in the hole, with around soil pressure real, water dilution back He Gelunte nutrient solution then at every turn, observe survival rate and get final product;
D or with step a diversiform-leaved poplar seed imbibition 8-12 hour, vernalization is sprouted in incubator, and temperature 25-28 ℃, relative moisture 30-50% cultivates after 3-5 days and mixes with the sandy soil of Glomus mosseae spore, and spreading is on preprepared culture bed sand bed surface;
E, overlay the sandy soil that one deck is rich in the Glomus mosseae spore below the culture bed sand bed of steps d, temperature is controlled at 20-25 ℃, will sprout in 5-10 days, sprouts the back 10-15 days, and root system will can be infected by being rich in the sandy soil layer of Glomus mosseae spore.
2. according to the described method of claim 1, it is characterized in that step b is 2-3mm at the sandy soil that bottom, hole spreading is rich in the Glomus mosseae spore.
3. according to the described method of claim 2, it is characterized in that the sandy soil of step b and steps d Glomus mosseae spore are the washed-out sand less than 2mm, 15-25 spore/ml sand.
4. want 3 described methods according to right, it is characterized in that overlaying below the culture bed sand bed 2-3cm of step e the sandy soil that one deck is rich in the Glomus mosseae spore is 2-3mm, and the gross thickness of culture bed sand bed is 20-50cm.
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| CN102613054A (en) * | 2012-04-09 | 2012-08-01 | 广东省生态环境与土壤研究所 | Method for improving cold and disease resistance of tobacco |
| CN103875503A (en) * | 2014-01-16 | 2014-06-25 | 中国科学院新疆生态与地理研究所 | Method for increasing survival rate in transplanting populus euphratica shoots in extremely arid desert area |
| CN105248145A (en) * | 2015-11-27 | 2016-01-20 | 中国科学院新疆生态与地理研究所 | Method for promoting growth of mongolian bluebeard by inoculating arbuscular mycorrhizal fungi |
| CN105284404A (en) * | 2015-11-27 | 2016-02-03 | 中国科学院新疆生态与地理研究所 | Method for promoting growth of Poacynum pictum by inoculating arbuscular mycorrhizal fungi |
| CN105379529A (en) * | 2015-11-11 | 2016-03-09 | 新疆林科院造林治沙研究所 | Semi-shading sapling raising method for populusdiversifolia |
| CN105432398A (en) * | 2015-11-19 | 2016-03-30 | 西南大学 | Method for increasing survival rate of mulberry trees under stress conditions of water logging and drought |
| CN106332573A (en) * | 2016-08-26 | 2017-01-18 | 新疆林科院造林治沙研究所 | Method for promoting germination of populus euphratica seeds |
| CN107278406A (en) * | 2017-07-21 | 2017-10-24 | 吉林省林业科学研究院 | A kind of method that utilization arbuscular mycorrhiza repairs saline-alkali bare land |
| CN110999703A (en) * | 2019-12-20 | 2020-04-14 | 宜兴乾元黑色食品科技有限公司 | Method for improving secondary metabolite content of vaccinium bracteatum by infecting with megasporocyst mould |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN114830870A (en) * | 2022-05-25 | 2022-08-02 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Application of arbuscular mycorrhizal fungi in germination and cuttage of gardenia seeds |
| CN115927158A (en) * | 2023-02-13 | 2023-04-07 | 西北农林科技大学 | Culture medium for tissue culture and rapid propagation of jujube trees, application of culture medium and rapid propagation method of mycorrhizal seedlings of jujube trees |
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| CN102613054A (en) * | 2012-04-09 | 2012-08-01 | 广东省生态环境与土壤研究所 | Method for improving cold and disease resistance of tobacco |
| CN103875503A (en) * | 2014-01-16 | 2014-06-25 | 中国科学院新疆生态与地理研究所 | Method for increasing survival rate in transplanting populus euphratica shoots in extremely arid desert area |
| CN105379529B (en) * | 2015-11-11 | 2017-09-15 | 新疆林科院造林治沙研究所 | The shading method for culturing seedlings of diversiform-leaved poplar half |
| CN105379529A (en) * | 2015-11-11 | 2016-03-09 | 新疆林科院造林治沙研究所 | Semi-shading sapling raising method for populusdiversifolia |
| CN105432398A (en) * | 2015-11-19 | 2016-03-30 | 西南大学 | Method for increasing survival rate of mulberry trees under stress conditions of water logging and drought |
| CN105284404B (en) * | 2015-11-27 | 2018-07-31 | 中国科学院新疆生态与地理研究所 | The method that Arbuscular Mycorrhizal Fungi promotes hemp growth |
| CN105284404A (en) * | 2015-11-27 | 2016-02-03 | 中国科学院新疆生态与地理研究所 | Method for promoting growth of Poacynum pictum by inoculating arbuscular mycorrhizal fungi |
| CN105248145A (en) * | 2015-11-27 | 2016-01-20 | 中国科学院新疆生态与地理研究所 | Method for promoting growth of mongolian bluebeard by inoculating arbuscular mycorrhizal fungi |
| CN106332573A (en) * | 2016-08-26 | 2017-01-18 | 新疆林科院造林治沙研究所 | Method for promoting germination of populus euphratica seeds |
| CN107278406A (en) * | 2017-07-21 | 2017-10-24 | 吉林省林业科学研究院 | A kind of method that utilization arbuscular mycorrhiza repairs saline-alkali bare land |
| CN110999703A (en) * | 2019-12-20 | 2020-04-14 | 宜兴乾元黑色食品科技有限公司 | Method for improving secondary metabolite content of vaccinium bracteatum by infecting with megasporocyst mould |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN114830870A (en) * | 2022-05-25 | 2022-08-02 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Application of arbuscular mycorrhizal fungi in germination and cuttage of gardenia seeds |
| CN115927158A (en) * | 2023-02-13 | 2023-04-07 | 西北农林科技大学 | Culture medium for tissue culture and rapid propagation of jujube trees, application of culture medium and rapid propagation method of mycorrhizal seedlings of jujube trees |
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