CN106613125A - Method for promoting side slope green retrieval through mycorrhiza inoculation - Google Patents
Method for promoting side slope green retrieval through mycorrhiza inoculation Download PDFInfo
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- CN106613125A CN106613125A CN201610987769.6A CN201610987769A CN106613125A CN 106613125 A CN106613125 A CN 106613125A CN 201610987769 A CN201610987769 A CN 201610987769A CN 106613125 A CN106613125 A CN 106613125A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a method for promoting side slope green retrieval through mycorrhiza inoculation. Firstly, stolons of plants undergo cuttage in a matrix mixed with arbuscular mycorrhizal fungi, and watering and maintenance are performed for propagation expanding; the plants obtained through the propagation expanding are taken out, the stolons are sheared into stem sections with adventitious roots and the matrix; fermentation broth of ectotrophic mycorrhiza, side slope plant seeds and an organic fertilizer for sterilization are taken and mixed to obtain a mixture; a base material is spray-seeded on a side slope; the obtained plant stem sections and the mixture are mixed and spray-seeded; arbor seedlings are transplanted, the ectotrophic mycorrhiza and/or the arbuscular mycorrhizal fungi are taken to wrap broken roots of the arbor seedlings or the ectotrophic mycorrhiza and/or the arbuscular mycorrhizal fungi is scattered to pits, the arbor seedlings are planted in the pits; the stem sections spray-seeded with a bacterium carrying agent can be used for inoculating the arbuscular mycorrhizal fungi to all the side slope plants, the side slope plants and the spray-seeded stolon section plants grow rapidly, the demand for rapid green retrieval at the earlier stage of side slope green retrieval can be met, mycorrhiza fungi inoculation is specifically conducted on the arbor seedlings, and the demand for keeping vegetation for a long period of the side slope can be met.
Description
Technical field
The present invention relates to Slope greening technical field, and in particular to a kind of method that mycorhiza inoculation promotes Slope greening.
Background technology
Hand excavation's side slope is depended on for existence due to the features such as the gradient is steep, slope is high, soil environment is severe, generally not possessing vegetation
Soil, moisture and nutrient condition so that slope vegetation restoration is relatively difficult.Further, since the infrastructure project such as road, water power side
Slope circuit length, area are big, and nourishment is more difficult, limited along with injecting capital into, and slope plant typically all in being conigenous certainly
Foster state.Simultaneously the conventional plant of sowing grass seeds by duster of side slope has certain selectivity and adaptability to weather and environment, and resistance itself is limited,
Low-maintenance or without easily degenerating in the case of maintenance, it is dead.Vegetation degeneration problem cannot be solved, and not only cause repeated construction, money
Gold is wasted, and does not have ecological protection effect, it is also possible to can cause new soil erosion and domatic destruction, in some instances it may even be possible to can lead
Cause the consequences such as slope failure.Side slope can be resolved and lack arid, height that soil, hydropenia, the state of fertilizer deficiency and side slope vegetation face
Temperature etc. is coerced, and will directly influence the final afforestation effect of side slope and ecological benefits.
The numerous researchs of Chinese scholars find that mycorrhizal fungi can improve the anti-adversity ability of plant, such as drought resisting, and salt tolerant is acidproof,
Low-temperature resistance, preventing from heavy metal murder by poisoning and diseases and insect pests resistance etc., while plant nutrient can be improved, such as interior raw clump branch bacterium is connected with root system
Mycelia net enable the soil volume that plant reaches to increase several orders of magnitude, greatly improve the efficiency of mycorhiza Root Absorption phosphorus.
Research shows ecological recovery and process of reconstruction of the mycorhiza in degraded ecosystems such as desert, arid biogeographic zone, mining area, salt-soda soil, meadows
In serve significant facilitation, can promote the growth of plant under adverse environmental factor, increase Plant Diversity etc..Arbuscular mycorrhiza
Funguses (Arbuscular mycorrhiza fungus, abbreviation AMF) are being total to for Glomeromycota fungal infection root system of plant formation
Raw body, without strict host specificity, can form arbuscular mycorrhiza with about more than 90% terrestrial plant on the earth.
Exotrophic mycorrhiza (Ectomycorrhiza, ECM) is the symbiotic structure that funguses and fine-root are formed in soil, artificial afforestration seeds
In have 70%～75% the exotrophic mycorrhiza that can be formed, Arbuscular Mycorrhizal Fungi bacterium nursery is remarkably improved the quality and survival rate of nursery stock,
Shorten seedling-slowing stage after afforestation.But, AMF has and plant height symbiosis, obligate nutritional character, it is more difficult to obtain pure
Culture, and Applying Ectomycorrhizal Fungi poor growth, these all constrain the production and application of bush mycorrhiza agent so that repair in actual side slope
It is difficult to promote the use of VA Mycorrhizal Fungi in journey of returning to work.A kind of simpler efficiently vaccination ways are provided, are had for VA Mycorrhizal Fungi is promoted
Very important meaning.
The content of the invention
The technical problem to be solved is to provide a kind of method that mycorhiza inoculation promotes Slope greening.
The technical solution used in the present invention is：
A kind of method that mycorhiza inoculation promotes Slope greening, comprises the following steps：
S1：By the stolon cuttage of plant in the substrate of AMF is mixed with, water seasoning expanding propagation；
S2：The plant that S1 expanding propagation is obtained is taken out, the stem section with adventitious root and the substrate is cut into along stolon；
S3：Fermentation liquid, slope plant seed and sterilizing fertilizer, the mixing of exotrophic mycorrhiza are taken, mixture is obtained；
S4：Sow grass seeds by duster in side slope base material, obtain the substrate layer being covered in side slope；
S5：Take the mixture that the plant stem section, S3 that S2 obtains is obtained, mixing, the base material in side slope of being sowed grass seeds by duster
On layer, plant microbial inoculum layer is obtained；
S6：Transplanting arbor Seedling, takes exotrophic mycorrhiza and/or AMF parcel arbor Seedling is completely cured or by exotrophic mycorrhiza
And/or AMF is sown in the cave hole in side slope, arbor Seedling is implanted in the cave hole.
In some specific embodiments, the plant be tunnel bed structure, runner bean, Trifolium repense and Bermuda grass in extremely
Few one kind.
In some specific embodiments, the AMF includes Glomus mosseae, Glomus intraradices, children's set
Sacculus is mould.
In some specific embodiments, the exotrophic mycorrhiza is Pisolithus tinctorius.
In some specific embodiments, the arbor Seedling is acacia confusa or appoints bean.
In some specific embodiments, also include taking plant stem section that base material obtains with S2 in the S5 and S3 is obtained
Mixture be mixed the step of sowing grass seeds by duster.
In some specific embodiments, the thickness of the plant microbial inoculum layer is 2-5cm.
In some specific embodiments, the thickness of the substrate layer is 10-15cm.
In some specific embodiments, the fermentation liquid of the exotrophic mycorrhiza is to be seeded to culture by Applying Ectomycorrhizal Fungi
Base shake flask fermentation is obtained.
The invention has the beneficial effects as follows：
The invention provides a kind of method that mycorhiza inoculation promotes Slope greening, the stolon cuttage of plant is being mixed with
In the substrate of AMF, then water seasoning expanding propagation is cut into the plant that expanding propagation is obtained with substrate and adventitious root
Stem section so that stem section of crawling contains AMF, then it mixed sow grass seeds by duster with exotrophic mycorrhiza fermentation liquid and fertilizer,
First, the seeded process is very simple, if by stolon cuttage in the substrate for be mixed with AMF expanding propagation, phase
For the inoculation method of traditional AMF, the difficulty of inoculation is greatly reduced, and increase the clump branch bacterium of inoculation
The time-to-live of mycorrhiza fungi, furthermore, using crawling, stem section is directly sowed grass seeds by duster, and the plant of side slope can be supplemented after plant survival, if
Plant fails to survive, also can be used as microbial inoculum, the growth and breeding of promotion side slope whole plant, and the 3rd, by AMF and outward
Raw mycorhiza fermentation liquid mixing is sowed grass seeds by duster, can the conventional arbor of side slope greening carry out Mixed inoculation, improve growth rate and resistance；
The root system of seedling will necessarily be damaged because transplanting the process of arbor Seedling during Slope greening, without the need for entering to seedling
Row is completely cured, and directly exotrophic mycorrhiza and/or AMF can be wrapped up into being completely cured or will grow thickly mycorhiza and/or clump for seedling
Mycorrhizal fungi is sown in the hole of cave, and arbor is inoculated with, and can very effectively improve the speed of growth of arbor, and this
The bright middle growth-promoting functions for nursery stock Mycorrhizal being combined with greening side slope, mycorrhizal fungi being given full play to so that seed sprouting
Rate, plant percent are greatly improved, and the shrub speed of growth also can be obviously improved, while also contributing to the tall grass that fills is combined group
Formed.Sum it up, the draft with microbial inoculum of sowing grass seeds by duster is crawled, stem section can be inoculated with clump branch bacterium, whole side slope draft to whole slope plants
Plant and the stem section quick growth of plant of crawling sowed grass seeds by duster, disclosure satisfy that greening side slope early stage replies immediately soon green demand, specificity
Mycorrhizal fungi Mixed inoculation must be carried out to arbor Seedling, contribute to afforestation, the demand that side slope needs keep for a long time vegetation can be met.
Description of the drawings
Fig. 1 is the mycorhiza observation result under the microscope of three kinds of plants.
Fig. 2 for Bermuda grass mycorhiza observation result under the microscope.
Specific embodiment
First, AMF inoculation test
Tunnel bed structure, the cuttage of runner bean stolon are being mixed with respectively in Glomus mosseae (Glomus mosseae), root
In sacculus mould (Glomus intraradices), the substrate of Glomus etunicatum (Glomus etunicatum) microbial inoculum, periodically pour
Water curing is covered with to potted plant.Tunnel bed structure root invasion rate is about 95.33 ± 1.58%, and runner bean root invasion rate is 91.67
± 0.53%, Jing wet screening decantation staining detection, the clump branch bacteria agent spore using cuttage tunnel bed structure and runner bean expanding propagation is close
About 60～90 spore/10g of degree or so.Experiment proves that mycorrhizal fungi is applied to side slope and mainly has following benefit.
2nd, exotrophic mycorrhiza inoculation arbor test
1st, plant growing can be promoted
Selection growing way is consistent, the close 25 ± 1.27cm of plant height of specification, the acacia confusa seedling of 2.8 ± 0.15mm of ground diameter,
It is divided into three groups to be tested, one group is matched group, and one group is a small amount of inoculation group, and one group is how bright inoculation group, and matched group is not inoculated with
Pisolithus tinctorius, transplant arbor Seedling, and seedling root is imperiled in migration process, and a small amount of inoculation group takes a small amount of exotrophic mycorrhiza
Parcel arbor Seedling is completely cured, and volume inoculation group is sown in volume exotrophic mycorrhiza in the hole of cave, and arbor Seedling is implanted in the cave hole, is connect
Plant Pisolithus tinctorius and determine three groups of acacia confusa increments after 5 months respectively, SPAD determines chlorophyll content.1 is the results are shown in Table, it is few
, because annual seedling is without branch, hat width difference is little, but plant height, height growth are notable for amount inoculation group and volume inoculation group
Higher than matched group, leaf color is bright-coloured, and chlorophyll content is also significantly greater than matched group.
The impact that the inoculation Pisolithus tinctorius of table 1 grow to acacia confusa
Note：It is diverse with column data shoulder mark lower case, represent significant difference (P<0.05), there is any identical small letter
Not notable (the P of letter or the expression difference without letter>0.05).Following table is same.
3rd, AMF inoculation arbor test
AMF inoculation test is divided into four groups and is tested, and Glomus mosseae, Glomus intraradices, children are carried out respectively
The mould single inoculation of set sacculus and blank control group, per group of six Duplicate Samples, first Jia 60% before plantation (mass fraction) sterilizing
Substrate；Then each group is separately added into the microbial inoculum of 20% (mass fraction), adds 10 to appoint bean seed and uniformly spread out per basin；Finally
20% (mass fraction) sterilization matrix is added to cover seed and pour permeable；Matched group substrate is the sterilization matrix of equal volume, often
6 repetitions of individual process.Process 2 weeks laggard Seedlings in the ranks to process, 3 plants are chosen per basin and appoints bean seedlings, take the plant of growing way uniformity.Appoint
After bean seedlings culture 6 months, increment statistics is carried out.Increment statistical data as shown in table 2, can see single connecing from table 2
After kind of any one AMF, the increment and chlorophyll content blank control group that all compares for appointing bean is substantially improved.
Table 2 is inoculated with clump branch bacterium to appointing the impact of bean growth
Appoint bean natural drought after 15 days, carry out plant drought resistance test, the result such as table 3 of drought resistance.Bean is appointed to do naturally
After 15 days, all bean leaf r elative water contents of appointing for being vaccinated with AMF show downward trend to drought, relatively electricity
Conductance, mda content, soluble sugar content increased.But Va Mycorrhiza Seedling leaf r elative water content, soluble sugar content are still
Matched group is significantly higher than, relative conductivity, mda content are substantially less than matched group, shows that Va Mycorrhiza Seedling has higher to drought stress
Tolerance, leaf abscission is relatively fewer, and the anti-membrane oxidation ability of plant cell is stronger, damaged membrane lesser extent, while
Osmoregulation class material is generated, shows Arbuscular Mycorrhizal Fungi Seedling to appointing bean drought resistance to be improved to some extent.
Table 3 is inoculated with clump branch bacterium to appointing the impact of bean drought resistance
Four groups of plant growing Soil Phosphorus acid enzymes, urease activities are analyzed respectively, obtain result such as table 4.Can from table 4
Arrive, three kinds are inoculated with respectively from after branch mycorhiza, the phosphatase and urease activity of soil are significantly increased.Phosphatase is non-in soil
Often important enzyme, its activity can be used as the index for evaluating soil phosphorus bioconversion direction and intensity.Soil urease liveness with
The micro organism quantity of soil, organic matter content, full nitrogen and available phosphorus contents are proportionate.
Table 4 is inoculated with the impact that clump branch bacterium converts related enzyme activity to soil nitrogen phosphorus
Note：Colleague's data shoulder mark lower case is diverse, represents significant difference (P<0.05), there is any identical small letter
Not notable (the P of letter or the expression difference without letter>0.05).
4th, side slope is sowed grass seeds by duster test
The stolon cuttage of tunnel bed structure, runner bean, Trifolium repense and Bermuda grass is being mixed with into the base of AMF
In matter, water seasoning expanding propagation；The plant that expanding propagation is obtained is taken out, the mycorhiza dyeing of tunnel bed structure, runner bean, Trifolium repense is taken, is placed in
Basis of microscopic observation, obtains Fig. 1, and Fig. 1 a are blank root systems, is the root system observation under the microscope of common tunnel bed structure
As a result, Fig. 1 b, Fig. 1 c, Fig. 1 d are respectively the mycorhiza observation results under the microscope of tunnel bed structure, runner bean, Trifolium repense, Fig. 2
For the mycorhiza observation result under the microscope of Bermuda grass, it can be seen that the Glomus mosseae contaminated in root system of Bermuda grass, lead to
Cross detection and find that tunnel bed structure, runner bean, Trifolium repense and Bermuda grass root system Infection Rate, up to more than 90%, are illustrated by stolon
In the substrate of AMF is mixed with, clump branch bacterium can be in the root of tunnel bed structure, runner bean, Trifolium repense and Bermuda grass for cuttage
It is colonized well in system, and infection rate is high.Sow grass seeds by duster in side slope base material, the substrate layer being covered in side slope is obtained, along stolon
It is cut into the stem section with adventitious root and the substrate；Take by Applying Ectomycorrhizal Fungi be seeded to culture media shaking vase fermentation obtain it is external
The fermentation liquid of mycorhiza, plant stem section, slope plant seed and sterilizing fertilizer, mixing, the base in side slope of being sowed grass seeds by duster
On material layer, plant microbial inoculum layer is obtained.
Bean, acacia confusa Seedling are appointed in transplanting, take exotrophic mycorrhiza and AMF parcel arbor Seedling is completely cured, and arbor Seedling is planted
In entering the cave hole, detection appoints bean, the root system Infection Rate of acacia confusa both can be formed up to more than 90%, Ren Dou, acacia confusa
Endo-mycorrhiza, can form exotrophic mycorrhiza again.After sowing grass seeds by duster, herbaceous plant Trifolium repense, runner bean and Bermuda grass fast-growth, side slope is fast
Reply immediately green, spring and summer is entered after shrub growth animated period, and withered and yellow phenomenon occurs in Bermuda grass, and Trifolium repense aerial partss are dead in a large number
Die, and appoint bean, acacia confusa to start swift and violent growth, plant height covered quickly thick grass height, realizes control grass and helps filling.
Claims (9)
1. a kind of method that mycorhiza inoculation promotes Slope greening, it is characterised in that comprise the following steps：
S1：By the stolon cuttage of plant in the substrate of AMF is mixed with, water seasoning expanding propagation；
S2：The plant that S1 expanding propagation is obtained is taken out, the stem section with adventitious root and the substrate is cut into along stolon；
S3：Fermentation liquid, slope plant seed and sterilizing fertilizer, the mixing of exotrophic mycorrhiza are taken, mixture is obtained；
S4：Sow grass seeds by duster in side slope base material, obtain the substrate layer being covered in side slope；
S5：Take the mixture that the plant stem section, S3 that S2 obtains is obtained, mixing, on the substrate layer sowed grass seeds by duster in side slope,
Obtain plant microbial inoculum layer；
S6：Transplanting arbor Seedling, take exotrophic mycorrhiza and/or AMF parcel arbor Seedling be completely cured or by exotrophic mycorrhiza and/or
AMF is sown in the hole of the cave in side slope, and arbor Seedling is implanted in the cave hole.
2. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that the plant is floor file
At least one in Folium et Caulis Indigoferae Tinctoriae, runner bean, Trifolium repense and Bermuda grass.
3. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that the arbuscular mycorrhiza is true
Bacterium bag includes Glomus mosseae, Glomus intraradices, Glomus etunicatum.
4. the method that mycorhiza according to claim 1 inoculation promotes Slope greening, it is characterised in that the exotrophic mycorrhiza is
Pisolithus tinctorius.
5. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that the arbor Seedling is platform
Gulf yearning between lovers appoints bean.
6. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that also include in the S5
Take the plant stem section that base material and S2 obtain and the mixture that S3 is obtained is mixed the step of sowing grass seeds by duster.
7. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that the plant microbial inoculum layer
Thickness be 2-5cm.
8. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that the thickness of the substrate layer
Spend for 10-15cm.
9. the method that mycorhiza inoculation according to claim 1 promotes Slope greening, it is characterised in that the exotrophic mycorrhiza
Fermentation liquid is seeded to culture media shaking vase fermentation and obtains by Applying Ectomycorrhizal Fungi.
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| CN109574720A (en) * | 2019-01-07 | 2019-04-05 | 德州学院 | A method of promoting poplar fast-growth |
| CN110122204A (en) * | 2019-05-06 | 2019-08-16 | 辽宁大学 | A kind of method on the steel slag mountain that original position restoration of the ecosystem northern area is piled based on discarded steel slag |
| CN111512824A (en) * | 2020-05-19 | 2020-08-11 | 神华神东煤炭集团有限责任公司 | Method for promoting growth of greening plants by using composite microbial inoculum |
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