CN116171857B - Method for efficiently rooting of Ficus simplicissima lour outside bottle - Google Patents
Method for efficiently rooting of Ficus simplicissima lour outside bottle Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 241001578027 Ficus simplicissima Species 0.000 title 1
- 244000173782 Ficus hirta Species 0.000 claims abstract description 10
- 241000124033 Salix Species 0.000 claims description 41
- 238000011282 treatment Methods 0.000 claims description 37
- 239000011159 matrix material Substances 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 22
- 230000006698 induction Effects 0.000 claims description 21
- 238000002791 soaking Methods 0.000 claims description 17
- 238000002156 mixing Methods 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- 235000016709 nutrition Nutrition 0.000 claims description 13
- 230000035764 nutrition Effects 0.000 claims description 13
- 235000019362 perlite Nutrition 0.000 claims description 12
- 239000010451 perlite Substances 0.000 claims description 12
- 238000000227 grinding Methods 0.000 claims description 11
- 239000008223 sterile water Substances 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 7
- 238000011161 development Methods 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 244000060011 Cocos nucifera Species 0.000 claims description 5
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 5
- 241000646858 Salix arbusculoides Species 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 239000003337 fertilizer Substances 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000004570 mortar (masonry) Substances 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 5
- 239000010802 sludge Substances 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 2
- 239000004021 humic acid Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000005416 organic matter Substances 0.000 claims description 2
- 241000203475 Neopanax arboreus Species 0.000 abstract description 14
- 241001631271 Prunus fasciculata Species 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000003672 processing method Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 12
- 239000000203 mixture Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000004745 nonwoven fabric Substances 0.000 description 4
- 230000007480 spreading Effects 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- 229940107666 astragalus root Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 241000644796 Ficus ficus Species 0.000 description 1
- 235000011591 Ficus hirta Nutrition 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 230000037006 agalactosis Effects 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 235000020997 lean meat Nutrition 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
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- 230000004083 survival effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The application relates to the technical field of seedling raising, in particular to a method for efficiently rooting of a five-finger wild peach outside a bottle. The processing method successfully realizes out-bottle rooting, greatly improves the tissue culture rooting efficiency of the Ficus simplicissima lour, saves the electric energy consumed in the tissue culture rooting stage, and provides a high-efficiency production mode for industrially producing Ficus simplicissima lour seedlings.
Description
[ field of technology ]
The application relates to the technical field of seedling raising, in particular to a method for efficiently rooting out of a five-finger wild peach bottle.
[ background Art ]
Ficus hirta Vahl.) is a common herb in the south of the Ling, and its plant is derived from the root of Ficus Ficus of Moraceae, and was first described in the "Shengcao Yao" in Qing Dynasty. It is produced in mountain forest shrubs, also called five-claw dragon, astragalus root, five-finger milk, astragalus root, etc. and is mainly distributed in Guangdong, guangxi, yunnan, guizhou and other places. It has effects of invigorating spleen, nourishing lung, invigorating qi, eliminating dampness, relieving cough and asthma, and treating anorexia and weakness, puerperal agalactia, etc. The five-finger wild peach is a road medicinal material with both medicine and food purposes, is used as a medicine, is also frequently used for cooking soup by lean meat, chicken, pig bone and other foods in folk, has higher edible value and larger market demand, and has a large development space.
Along with the rapid development of industrial seedling culture, tissue culture technology has been widely used as an important means for plant rapid propagation. In the conventional tissue culture process, the processes of explant acquisition and inoculation, subculture, rooting culture, transplanting, domestication and the like are generally required. In the rooting and transplanting stage, the problems of lower rooting rate, weaker root system, difficult survival in transplanting and the like of part of plants exist.
Based on the above, the application provides a method for efficiently rooting the outer part of the five-finger wild peach bottle, so as to solve the problems.
[ application ]
In view of the above, it is necessary to provide a method for efficiently rooting the hispid fig outside the bottle, which successfully realizes rooting outside the bottle, greatly improves the tissue culture rooting efficiency of the hispid fig, and provides an efficient production mode for industrially producing the hispid fig seedlings.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
a method for efficiently rooting of Ficus simplicissima ex lour outside a bottle comprises the following steps:
(1) Preparing a rooting matrix: mixing perlite and sand according to the volume ratio of 3-5:1, putting into water, boiling, maintaining for 4-6 minutes, and fishing out for standby;
(2) Preparing a transplanting matrix: uniformly mixing pond sludge, coconut coir, a seedling culture matrix and perlite according to the volume ratio of 2:1:1:1, and filling the mixture into a nutrition cup for later use; wherein, the nutrition cup is a non-woven fabrics nutrition cup, and its model is: 9cm 11cm;
(3) Out-bottle rooting induction: shearing the tissue culture Miao Shan strain of Ficus simplicissima, soaking the basal part in the treatment solution for 15-25min, taking out and inserting into rooting matrix for culturing at 25-28deg.C under 8-12h/d, spraying water once every 1-2 days with a spray can, keeping matrix moist, and observing rooting condition;
(4) Transplanting and raising seedlings; transplanting the plants subjected to the out-bottle rooting induction culture in the step (3) for 20-25 days into a transplanting matrix, and performing conventional water and fertilizer management.
Further, the treatment solution in the step (3) is obtained by mixing an ABT solution and a willow branch solution according to a volume ratio of 1:1.
Further, the ABT solution is prepared by measuring 1.0mg of ABT rooting powder and dissolving the ABT rooting powder in 1L of sterile water; the ramulus Salicis Babylonicae liquid is prepared by grinding 20mg ramulus Salicis Babylonicae with a mortar to paste, adding 100ml sterile water while grinding, stirring thoroughly, filtering with 40 mesh bag, and removing residue.
Wherein ABT is R8240 rooting powder produced by Beijing Soy Bao technology Co.
Further, the willow branches are selected from willow twigs which are just germinated in the spring of the trimester.
Furthermore, the seedling raising substrate is a full-value seedling raising substrate of Saishi brand produced by Saishi agriculture development Limited liability company, wherein the organic matter and humic acid are more than 50 percent.
And (2) in the step (1), the perlite and the sand are fished out of boiling water and then are cooled, and then are laid in a seedling tray, so that a rooting matrix with the thickness of 10cm is obtained.
The application has at least the following beneficial effects:
1. compared with the traditional plant tissue culture, the method omits the process of transplanting and hardening off the seedling after rooting in the bottle, and based on the method, the method for rooting the outer root of the five-finger wild peach bottle is provided for the first time, and sectional culture is adopted, so that rooting culture and transplanting, domestication and seedling raising are combined, the seedling raising period is shortened, the production cost is saved, and the method is worthy of popularization.
2. Although the rooting outside the bottle has a plurality of advantages, for example, in the process of rooting outside the bottle and raising seedlings, the selection of rooting matrix has great influence on rooting rate and rooting quality, according to the cultivation experience of the five-finger wild peach for many years, the applicant tries to perform rooting outside the bottle on the five-finger wild peach, although the raising period can be shortened, the rooting rate and rooting quality of the five-finger wild peach are poorer than those of rooting inside the bottle, so how to adjust the cultivation method to realize rooting outside the bottle is the subject of the applicant's previous study, and through the combination of the cultivation experience of multiple experiments, the applicant finds that the soaking solution obtained by combining the willow twig liquid prepared from the willow twigs which are just germinated in the spring of the year and rooting powder with corresponding concentration is adopted for treating the tissue culture seedling base, so that the tissue culture rooting efficiency of the five-finger wild peach can be greatly improved, meanwhile, the electric energy consumed in the tissue culture rooting stage is saved, and an efficient production mode is provided for the industrialized production of the five-finger wild peach seedlings.
[ description of the drawings ]
FIG. 1 is a graph showing rooting induction conditions of CK groups, treatment 2, treatment 4 and treatment 7 after rooting induction culture for 20 days in test one;
FIG. 2 is a plot of the vigor of CK group, treatment 2, treatment 4 and treatment 7 15 days after seedlings were transplanted in test one.
[ detailed description ] of the application
The following detailed description of the present application will provide further details in order to make the above-mentioned objects, features and advantages of the present application more comprehensible. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. The application may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit or scope of the application, which is therefore not limited to the specific embodiments disclosed below.
Example 1:
the embodiment is a method for efficiently rooting out of a five-finger wild peach bottle, which comprises the following steps:
(1) Preparing a rooting matrix: mixing perlite and sand according to the volume ratio of 3:1, putting into water, boiling, maintaining for 4 minutes, taking out, radiating, and then spreading the mixture in a seedling tray to obtain a rooting matrix with the thickness of 10 cm;
(2) Preparing a transplanting matrix: uniformly mixing pond sludge, coconut coir, a seedling culture matrix and perlite according to the volume ratio of 2:1:1:1, and filling the mixture into a nutrition cup for later use; wherein, the nutrition cup is a non-woven fabrics nutrition cup, and its model is: 9cm 11cm; the seedling raising substrate is a full-value seedling raising substrate of 'Saishi brand' produced by Changchun Saishi agriculture development Limited liability company;
(3) Out-bottle rooting induction: shearing the tissue culture Miao Shan strain of Ficus simplicissima, soaking the basal part in the treatment solution for 15min, taking out and inserting into rooting matrix for culturing at 25deg.C under 8h/d, spraying water once every 1 day with a spray can, keeping matrix moist, and observing rooting condition; the treatment fluid is prepared by mixing an ABT solution and a willow branch solution according to the volume ratio of 1:1; the ABT solution is prepared by weighing 1.0mg of ABT rooting powder and dissolving the ABT rooting powder in 1L of sterile water; the willow branch liquid is prepared by grinding 20mg of willow branch into paste with a mortar, adding 100ml of sterile water while grinding, fully stirring, filtering with a 40 mesh bag, and removing residues; the willow branches are selected from willow twigs which are just germinated in the spring of the three months;
(4) Transplanting and raising seedlings; transplanting the plants subjected to the out-bottle rooting induction culture in the step (3) for 22 days into a transplanting matrix, and performing conventional water and fertilizer management.
Example 2:
the embodiment is a method for efficiently rooting out of a five-finger wild peach bottle, which comprises the following steps:
(1) Preparing a rooting matrix: mixing perlite and sand according to the volume ratio of 4:1, putting into water, boiling, maintaining for 5 minutes, taking out, radiating, and then spreading the mixture in a seedling tray to obtain a rooting matrix with the thickness of 10 cm;
(2) Preparing a transplanting matrix: uniformly mixing pond sludge, coconut coir, a seedling culture matrix and perlite according to the volume ratio of 2:1:1:1, and filling the mixture into a nutrition cup for later use; wherein, the nutrition cup is a non-woven fabrics nutrition cup, and its model is: 9cm 11cm; the seedling raising substrate is a full-value seedling raising substrate of 'Saishi brand' produced by Changchun Saishi agriculture development Limited liability company;
(3) Out-bottle rooting induction: shearing the tissue culture Miao Shan strain of Ficus simplicissima, soaking the basal part in the treatment solution for 20min, taking out and inserting into rooting matrix for culturing at 26 deg.C under 10h/d, spraying water once every 1 day with a spray can, keeping matrix moist, and observing rooting condition; the treatment fluid is prepared by mixing an ABT solution and a willow branch solution according to the volume ratio of 1:1; the ABT solution is prepared by weighing 1.0mg of ABT rooting powder and dissolving the ABT rooting powder in 1L of sterile water; the willow branch liquid is prepared by grinding 20mg of willow branch into paste with a mortar, adding 100ml of sterile water while grinding, fully stirring, filtering with a 40 mesh bag, and removing residues; the willow branches are selected from willow twigs which are just germinated in the spring of the three months;
(4) Transplanting and raising seedlings; transplanting the plants subjected to the out-bottle rooting induction culture in the step (3) for 20 days into a transplanting matrix, and performing conventional water and fertilizer management.
Example 3:
the embodiment is a method for efficiently rooting out of a five-finger wild peach bottle, which comprises the following steps:
(1) Preparing a rooting matrix: mixing perlite and sand according to the volume ratio of 5:1, putting into water, boiling, maintaining for 6 minutes, taking out, radiating, and then spreading the mixture in a seedling tray to obtain a rooting matrix with the thickness of 10 cm;
(2) Preparing a transplanting matrix: uniformly mixing pond sludge, coconut coir, a seedling culture matrix and perlite according to the volume ratio of 3:2:2:1, and filling the mixture into a nutrition cup for later use; wherein, the nutrition cup is a non-woven fabrics nutrition cup, and its model is: 9cm 11cm; the seedling raising substrate is a full-value seedling raising substrate of 'Saishi brand' produced by Saishi agriculture development Limited liability company;
(3) Out-bottle rooting induction: shearing the tissue culture Miao Shan strain of Ficus simplicissima, soaking the basal part in the treatment solution for 25min, taking out and inserting into rooting matrix for culturing at 28deg.C under 12h/d, spraying water once every 2 days, keeping matrix moist, and observing rooting condition; the treatment fluid is prepared by mixing an ABT solution and a willow branch solution according to the volume ratio of 1:1; the ABT solution is prepared by weighing 1.0mg of ABT rooting powder and dissolving the ABT rooting powder in 1L of sterile water; the willow branch liquid is prepared by grinding 20mg of willow branch into paste with a mortar, adding 100ml of sterile water while grinding, fully stirring, filtering with a 40 mesh bag, and removing residues; the willow branches are selected from willow twigs which are just germinated in the spring of the three months;
(4) Transplanting and raising seedlings; transplanting the plants subjected to the out-bottle rooting induction culture in the step (3) for 25 days into a transplanting matrix, and performing conventional water and fertilizer management.
And (3) effect verification:
to illustrate the effect of the application, the applicant has carried out the following tests:
test one: comparing rooting induction conditions under different treatment fluids
In the experiment, rooting induction conditions of the treatment liquids of different groups after the treatment of the base parts of the hispid fig tissue culture seedlings are compared, and each group of treatment liquids and corresponding marks are shown in table 1:
table 1 corresponding numbers for each treatment of the soak solutions
| Soaking liquid | Corresponding process number |
| Clean water | CK |
| 0.5mg/l ABT solution | 1 |
| 1.0mg/l ABT solution | 2 |
| 2.0mg/lABT solution | 3 |
| 20mg/100ml willow branch liquid | 4 |
| 40mg/100ml willow branch liquid | 5 |
| 60mg/100ml willow branch liquid | 6 |
| 1.0mg/lABT+20mg/100ml willow branch liquid | 7 |
| 2.0mg/lABT+20mg/100ml willow branch liquid | 8 |
| 1.0mg/lABT+40mg/100ml willow branch liquid | 9 |
Wherein, 0.5mg/L of ABT solution, 1.0mg/L of ABT solution and 2.0mg/L of ABT solution are respectively obtained by dissolving 0.5mg, 1.0mg and 2.0mg of ABT rooting powder in 1L of water, and 20mg/100ml of willow branch liquid, 40mg/100ml of willow branch liquid and 60mg/100ml of willow branch liquid are respectively obtained by dissolving 20mg, 40mg and 60mg of willow branches after grinding in 100ml of sterile water and filtering to obtain filtrate.
The above-mentioned soaking solutions were sequentially taken, the culture of the hispid fig tissue culture seedlings was performed in the manner described in example 2 (except for the soaking solutions, the other culture modes of each group were identical), rooting conditions of each group of seedlings were observed during the culture, and the recorded data are shown in table 2:
TABLE 2 rooting induction conditions for different soaking treatments
As shown in Table 2, the rooting rate of the direct soaking in clear water is only 8.33%, the rooting rate of the soaking treatment with ABT rooting powder and willow branch liquid is obviously increased, the average rooting rate is over 4 pieces, the rooting rate of 1.0mg/l ABT solution is highest in 3 solutions with different concentrations of ABT rooting powder, the rooting rate reaches 85%, the average rooting rate reaches 9.20 pieces, 8 days are required for starting the rooting time, the rooting rate of 20mg/100ml willow branch solution also reaches 83.33% in 3 soaking treatments of the willow branch solution, the rooting rate of the average rooting rate reaches 9.80 pieces, and 8 days are required for starting the rooting time. Among the 9 treatments, the root emergence rate of the 1.0mg/l ABT+20mg/100ml willow branch solution soaking treatment 7 is highest and reaches 91.66%, the average rooting number also reaches 10.72, the rooting time is early, the rooting is started only 6 days, and 2-6 days earlier than other treatments.
After the radix induction culture is carried out on the hispid fig seedlings for 20 days, the rooting induction conditions of the CK group, the treatment 2, the treatment 4 and the treatment 7 are shown in the figure 1, then the second stage transplanting seedling is carried out, the growth vigor of the CK group, the treatment 2, the treatment 4 and the treatment 7 are shown in the figure 2 after 15 days of transplanting seedling, and obviously, the culture effect of the treatment 7 is better than that of other groups no matter in the rooting induction culture stage or the transplanting seedling stage.
And (2) testing II: comparing rooting induction conditions after different willow branch liquid soaking treatments
The applicant further compares the rooting induction conditions of the willow branch liquid prepared by the willow branches with different ages, and the other modes are identical except for the age of the willow branches, and the willow branches are cultivated by the method described in the example 2, and the results are shown in the table 3:
TABLE 3 rooting induction conditions of different age willow branch liquid soaking treatment
Wherein, the tender branch is the tender branch of the willow which is just germinated in the spring of the current year, and the old branch is the willow branch which grows for one year.
As can be seen from Table 2, the root emergence rate of the salix pentadactyla subculture single plant seedling of the wicker solution soaked by the just-germinated twigs reaches 85%, the average rooting number is 8.59, and the method is remarkably higher than the group of the wicker solution soaking treatment prepared by selecting the wicker of the old one year.
The above examples only represent a few embodiments of the present application, which are described in more detail and are not to be construed as limiting the scope of the present application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. Accordingly, the scope of the application should be assessed as that of the appended claims.
Claims (3)
1. The method for efficiently rooting the hispid fig outside the bottle is characterized by comprising the following steps of:
(1) Preparing a rooting matrix: mixing perlite and sand according to the volume ratio of 3-5:1, putting into water, boiling, maintaining for 4-6 minutes, and fishing out for standby;
(2) Preparing a transplanting matrix: uniformly mixing pond sludge, coconut coir, seedling culture matrix and perlite according to the volume ratio of 2-3:1-2:1-2:1, and filling into a nutrition cup for standby; the seedling raising substrate is a full-value seedling raising substrate of Saishi brand produced by Saishi agriculture development Limited liability company, wherein the organic matter and humic acid are more than 50%;
(3) Out-bottle rooting induction: shearing the tissue culture Miao Shan strain of Ficus simplicissima, soaking the basal part in the treatment solution for 15-25min, taking out and inserting into rooting matrix for culturing at 25-28deg.C under 8-12h/d, spraying water once every 1-2 days with a spray can, keeping matrix moist, and observing rooting condition;
(4) Transplanting and raising seedlings; transplanting the plants subjected to the out-bottle rooting induction culture in the step (3) for 20-25 days into a transplanting matrix, and performing conventional water and fertilizer management;
the treatment fluid in the step (3) is prepared from an ABT solution and a willow branch solution according to the volume ratio of 1:1, mixing the materials in proportion to obtain the product; the ABT solution is prepared by weighing 1.0mg of ABT rooting powder and dissolving the ABT rooting powder in 1L of sterile water; the ramulus Salicis Babylonicae liquid is prepared by grinding 20mg ramulus Salicis Babylonicae with a mortar to paste, adding 100ml sterile water while grinding, stirring thoroughly, filtering with 40 mesh bag, and removing residue.
2. The method of claim 1, wherein said willow shoots are selected from the group consisting of willow shoots that have just been germinated in the spring of the trimester.
3. The method of claim 1, wherein perlite and sand are fished out of boiling water in the step (1) and then cooled, and then are laid flat in a seedling tray to obtain a rooting matrix with the thickness of 10 cm.
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