CN110881321B - Cutting propagation method for upright stems of rhizoma phragmitis of amomum villosum - Google Patents

Cutting propagation method for upright stems of rhizoma phragmitis of amomum villosum Download PDF

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CN110881321B
CN110881321B CN201911175924.4A CN201911175924A CN110881321B CN 110881321 B CN110881321 B CN 110881321B CN 201911175924 A CN201911175924 A CN 201911175924A CN 110881321 B CN110881321 B CN 110881321B
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cutting
amomum villosum
reed
upright
propagation
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CN110881321A (en
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何国振
岳大明
邱科颖
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Guangzhou Kuotu Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00—Vegetative propagation
    • A01G2/10—Vegetative propagation by means of cuttings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material

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Abstract

The invention belongs to the technical field of propagation of amomum villosum, and particularly relates to a cutting propagation method for a reed head upright stem of the amomum villosum, which aims to better utilize materials of all parts of an amomum villosum plant in actual production seedling culture, enrich seedling culture means of the amomum villosum and solve the seedling problem of the amomum villosum‑1The NAA is used for cutting the shoots, then the cutting shoots are vertically inserted into a cutting medium which consists of nutrient soil, garden soil and perlite and is thoroughly watered, and finally the upright stems of the spring sand are successfully propagated through management after the cutting, so that a cutting propagation system of the upright stems of the spring sand is preliminarily established, technical support is provided for the rapid cutting propagation of the spring sand in the future, seedling raising means of the spring sand can be enriched, the seedling problem of the spring sand is solved, and the NAA has positive significance for the production of the spring sand.

Description

Cutting propagation method for upright stems of rhizoma phragmitis of amomum villosum
Technical Field
The invention belongs to the technical field of the propagation of amomum villosum, and particularly relates to a cutting propagation method of a reed head upright stem of amomum villosum.
Background
Yangchun sand (Yangchun sand)Amomum villosumLour.) belongs to the genus Amomum of the family Zingiberaceae (Zingibergeraceae) (AmomumRoxb.), is perennial evergreen herbaceous plant, the part used as the medicine is the dry and mature fruit of the perennial evergreen herbaceous plant, also called the fructus amomi chun, and is one of the famous four southern medicines in China. Yangchun Sha has the functions of resolving dampness and promoting appetite, warming spleen and checking diarrhea, regulating qi and preventing miscarriage, and is mainly used for treating qi stagnation of spleen and stomach, indigestion, abdominal pain and distension. At present, the Yangchun sand is mainly distributed in Guangdong, Guangxi, Yunnan and Fujian, etc., wherein the quality of Guangdong Yangchun is the best. The amomum villosum is a traditional bulk common medicinal material in China, has an application history of over 1300 years, has large market demand and is estimated to exceed 2.2 multiplied by 10 every year6kg, the market demand of the related industries exceeds 100 billion Yuan RMB. The yield of the domestic amomum villosum is about 1.6 multiplied by 106kg, far from meeting the market demand. The main reasons for low yield of the amomum villosum are high fruit drop rate, low natural fruiting rate, high artificial pollination strength and the like. In addition, the field environment and germplasm type of amomum villosum can also affect the yield.
The seedling source is a key link of production, and the conventional breeding method of the amomum villosum mainly comprises two modes of seed breeding and plant division breeding at present. The defects of seed propagation are that the germination time of the seeds is long, the service life of the seeds is short, and the seeds are difficult to store, and the defect of the plant division propagation is that the propagation coefficient is low. In order to better utilize materials of each part of the plant of the amomum villosum in the actual production seedling culture, enrich the seedling culture means of the amomum villosum and solve the seedling problem of the amomum villosum, a new method for propagating the amomum villosum, namely cutting propagation, needs to be established, and currently, the research on the cutting propagation by taking the upright stem of the reed head of the amomum villosum as the cutting shoot is not reported.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide a cutting propagation method for the upright stems of the spring festival amomum.
The invention also provides an application of the cutting propagation method of the reed rhizome upright stems of the amomum villosum in the propagation of the amomum villosum seedlings.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention provides a cutting propagation method for upright stems of rhizoma phragmitis of amomum villosum, which comprises the following steps:
s1, cutting: cutting the reed head upright stem of the amomum villosum as the cutting, placing the cutting in the concentration of 100--1Soaking in NAA (alpha-naphthylacetic acid) treating solution for 2 hr;
s2, a cutting method: thoroughly watering a cutting medium by water, and vertically inserting the soaked cutting slips into the cutting medium, wherein the cutting medium is formed by mixing nutrient soil, garden soil and perlite;
if the cuttage matrix is watered thoroughly with tap water, tap water placed overnight should be used, because the tap water just collected contains a small amount of chlorine, and is not beneficial to plant growth after long-term use.
And S3, managing after insertion. The method specifically comprises the following steps: after cuttage, the cuttage seedlings are protected from direct sunlight (such as cuttage in a sunny shed or other places without direct sunlight), and watering is carried out properly according to the humidity of the cuttage matrix so as to keep the cuttage matrix moist and remove weeds timely.
In order to better utilize materials of all parts of the plant of the amomum villosum in the actual production and seedling culture, the invention takes the reed-head upright stem of the amomum villosum as the cutting shoot for cutting propagation, researches the influence of NAA with different concentrations on the growth of the cutting shoot, and discovers that the reed-head upright stem of the amomum villosum passes through 100--1The NAA treatment can obtain good cuttage effect, and compared with the NAA treatment without NAA treatment, the indexes such as survival rate, rooting rate, budding rate and the like are obviously improved; the influence of the cutting matrixes with different formulas on the growth of the cutting slips is researched, and the cutting matrixes consisting of nutrient soil, garden soil and perlite are found to be capable of remarkably improving the survival rate of the cutting slips; the influence of different cutting modes on the growth of the cutting slips is researched, the vertical cutting mode is found to be more favorable for improving the survival rate of the cutting slips, and the fact that the reed-headed upright stems of the amomum villosum can be effectively subjected to cutting propagation is shown.
Preferably, the reed head upright stem is collected from a two-year-old reed head and an upright stem above the reed head, and the reed head upright stem at the stage has higher growth substance content, strong metabolic activity and easier survival.
Preferably, before the cutting shoot is treated, the lower end of the reed head part and the upper end of the upright stem part of the reed head upright stem are cut flat to form a cutting shoot with the length of 25-30 cm (as shown in figure 1), and the cutting shoot with the length of 25-30 cm is reserved to be more beneficial to the germination and growth of the cymbidium sinensis reed head upright stem.
Preferably, the concentration of the NAA treatment solution is 100-200 mg.L-1The study shows that the upright stem of the euonymus japonicus selenka is 200 mg.L-1After the NAA treatment fluid is treated, the survival rate is the best, and reaches 96.67%, and other growth indexes are also improved most obviously under the treatment of the concentration.
Preferably, the mixing weight ratio of the nutrient soil, the garden soil and the perlite is 2-4:2-4:1, more preferably, the mixing weight ratio of the nutrient soil, the garden soil and the perlite is 3:3:1, and researches show that the cutting matrix composed of the formula better accords with the growth habit of the upright stem of the head of the spring sand reed and is more beneficial to the survival of the upright stem of the head of the spring sand reed.
Preferably, the vertical insertion depth of the reed-head upright stem is determined to be appropriate to bury the reed head, and researches show that the reed head part of the reed-head upright stem is close to the ground, the ground temperature is high, the air is circulated, the healing and rooting are easy, and the survival of the reed-head upright stem is more facilitated.
Preferably, the cutting medium is subjected to solarization and disinfection in advance before cutting so as to prevent the rot of the cutting shoot caused by germ infection and influence the survival rate of the cutting shoot.
The invention also provides application of the eulexica officinalis tip upright stem cutting propagation method in eulexica officinalis seedling propagation.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a cutting propagation method for upright stems of reed heads of amomum villosum, which comprises the steps of cutting shoot treatment, a cutting method and management after cutting, and specifically comprises the following steps: cutting the erect stem of the reed head of the amomum villosum as the cutting shoot, firstly utilizing 100-500 mg.L-1The NAA is used for cutting shoot treatment, then the cutting shoot is vertically inserted into a cutting medium which is composed of nutrient soil, garden soil and perlite and is thoroughly watered, and finally the reed-headed upright stem of the amomum villosum is successfully propagated through management after insertion, so that a cutting propagation system of the upright stem of the reed-headed upright stem of the amomum villosum is initially established, and a technical support is provided for the rapid cutting propagation of the amomum villosum in the future. The cuttage propagation is simple to operate and high in propagation speed, compared with the plant division propagation, the cuttage propagation is light in used cuttage materials and small in occupied area, a plurality of problems in transportation can be effectively solved, meanwhile, the cuttage materials can adopt the amomum villosum roots and stems coming out of a garden, and the 'waste' resource utilization is realized. Therefore, the cutting propagation can enrich the seedling raising means of the amomum villosum, solve the seedling problem of the amomum villosum and has positive significance for the production of the amomum villosum.
Drawings
FIG. 1 shows the shape and length of the cutting;
FIG. 2 shows the effect of different NAA concentrations, different cutting media and different cutting methods on the survival rate of upright stems of amomum villosum;
FIG. 3 shows the effect of different NAA concentrations on the rooting rate and the sprouting rate of the upright stems of the spring sand reed heads;
fig. 4 shows the budding condition of the upright reed stems of the amomum villosum after cuttage.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
A cutting propagation method for upright stems of rhizoma Phragmitis of amomum villosum comprises the following steps:
s1, cutting: collecting rhizoma Phragmitis and upright stem (i.e. upright stem of rhizoma Phragmitis) of YANGCHUNSHA, cutting the lower end of rhizoma Phragmitis and the upper end of upright stem to obtain 28 cm long cutting, and placing the cutting at concentration of 0 mg.L-1Soaking in NAA treatment solution for 2 h;
s2, a cutting method: the cutting medium is formed by mixing nutrient soil, garden soil and perlite, and the mixing weight ratio of the nutrient soil, the garden soil and the perlite is as follows: nutrient soil: garden soil: 3:3:1, insolating and disinfecting a cutting matrix, subpackaging the cutting matrix into plastic round flowerpots (the caliber is 25 cm, the depth is 30 cm), thoroughly watering the cutting matrix by tap water placed overnight, vertically inserting the cutting slips after soaking treatment into the cutting matrix, properly setting the vertical inserting depth to be that the reed heads are buried, burying 1 cutting slip in each pot, and repeating 3 groups, wherein each group comprises 10 pots;
s3, management after insertion: placing the flowerpot in a sunshade after cuttage, preventing the flowerpot from being directly sunned by sunlight, properly watering according to the humidity of a cuttage matrix to keep the cuttage matrix moist, and timely removing weeds;
s4, counting the results: and observing the external form change of the cutting slips once a week after cutting, and counting the survival rate, the rooting rate, the budding rate and other growth indexes of the cutting slips after two months. Root diameter was measured with a vernier caliper at 2 mm from the root base. The experimental data are expressed as ` x. + -. s, and are counted and analyzed by using SPSS 25.0 software.
Example 2
The difference between example 2 and example 1 is: the concentration of NAA treatment solution was varied, and in example 1, 0 mg. multidot.L was used-1The NAA treatment solution of (1) in example 2 was prepared in an amount of 100 mg. multidot.L-1The NAA treatment solution of (4).
Example 3
The difference between example 3 and example 1 is: the concentration of NAA treatment solution was varied, and in example 1, 0 mg. multidot.L was used-1The NAA treatment solution of (1) in example 3 was 200 mg. multidot.L-1The NAA treatment solution of (4).
Example 4
The difference between example 4 and example 1 is: the concentration of NAA treatment solution was varied, and in example 1, 0 mg. multidot.L was used-1The NAA treatment solution of (1) in example 4 was 300 mg. multidot.L-1The NAA treatment solution of (4).
Example 5
The difference between example 5 and example 1 is: the concentration of NAA treatment solution was varied, and in example 1, 0 mg. multidot.L was used-1The NAA treatment solution of (1) in example 5 used 500 mg. multidot.L-1The NAA treatment solution of (4).
Example 6
The difference between example 6 and example 3 is: the cuttage matrix has different formulas, which are particularly shown in the component difference of the cuttage matrix, and the cuttage matrix adopted in the embodiment 6 consists of nutrient soil, peat soil and perlite, and specifically comprises the following components: nutrient soil: peat soil: perlite =3:3: 1.
Example 7
The difference between example 7 and example 3 is: the formula of the cutting medium is different, which is specifically shown in that the proportion of the cutting medium is different, and the proportion of the cutting medium adopted in the embodiment 7 is as follows: nutrient soil: garden soil: perlite is 3:1: 3.
Example 8
The difference between example 8 and example 3 is: the cuttage mode is different, the cuttage mode adopted by the embodiment 8 is a horizontal burying cuttage mode, and the cuttage depth is 2.5 cm.
As shown in the statistical results of survival rate in FIG. 2, it can be seen from examples 1 to 5 that the survival rate of the cuttings treated with NAA was significantly improved compared to the cuttings not treated with NAA (the survival rate was 73.33%). With the increase of the concentration of the NAA, the survival rate of the cutting shows a parabolic trend from low to high to low, when the concentration of the NAA is 200 mg.L-1 In the meantime, the survival rate of the cutting reaches the highest point, which is 96.67%. With the increase of the concentration of NAA, the survival rate of the cutting is reduced, and the analysis reason is that the high concentration of NAA breaks the hormone maintenance mechanism of the plant, so that the balance of the hormone level in the plant is disturbed, and the survival rate is influenced. It can be seen from examples 3, 6 and 7 that the formula of the cutting medium also has an important influence on the survival rate of the upright stems of the rhizoma amomi villosa, and the cutting medium consisting of nutrient soil, garden soil and perlite better conforms to the growth habit of the upright stems of the rhizoma amomi villosa and is more beneficial to the survival of the upright stems of the rhizoma amomi villosa. From the examples 3 and 8, it can be seen that the cutting mode also has an important influence on the survival rate of the upright stems of the amomum villosum, and the adoption of the vertical cutting mode is more beneficial to improving the survival rate of the upright stems of the amomum villosum.
As can be seen from the statistical results of the rooting rate and the germination rate in FIG. 3, the rooting rate and the germination rate of the cuttings treated with NAA are both improved to a certain extent compared with the cuttings not treated with NAA. Along with the increase of the concentration of the NAA, the rooting rate and the budding rate of the cutting shoot show a parabolic trend from low to high to low, when the concentration of the NAA is 200 mg.L-1When the method is used, the rooting rate and the germination rate of the cutting shoot reach the highest points, and are respectively 56.67% and 70.00%. The budding condition of the upright stems of the amomum villosum after cuttage is shown in figure 4.
Figure DEST_PATH_IMAGE001
Note: the average followed by the same letter indicates no significant difference at the 0.05 level.
As can be seen from the statistical results in Table 1, on averageIn the aspect of bud number, no significant difference exists after NAA treatment; only 200 mg.L of the average bud length-1The NAA treatment achieves a remarkable promoting effect; in terms of bud diameter, NAA has a certain promoting effect on bud diameter, but only 100 mg.L-1And 200 mg. L-1To a significant differential level, especially at 200 mg.L-1NAA of (a) is most significant; the longest bud length is 100 mg.L-1And 200 mg. L-1The NAA of (2) shows a remarkable accelerating effect, particularly at 200 mg.L-1NAA of (a) is most significant; in total, 100 mg.L-1And 200 mg. L-1The NAA has remarkable promoting effect on the average bud number, average bud length, bud diameter and longest bud length of the upright stem of the rhizoma Phragmitis of amomum villosum, especially 200 mg.L-1The NAA effect is best.
In conclusion, the upright stems of the rhizoma Phragmitis of the amomum villosum can obtain good cuttage effect after NAA treatment with different concentrations, the indexes such as survival rate, rooting rate and germination rate are obviously improved compared with those without NAA treatment, the survival rate, rooting rate and germination rate of the cutting slips show a parabolic trend from low to high to low with the improvement of the NAA concentration, wherein 200 mg.L-1Is the optimal NAA treatment concentration for the cutting propagation of the upright stems of the euonymus japonicus head. Meanwhile, the cutting medium and the cutting mode also have important influence on the survival rate of the upright stems of the euscaphis konishii, and the cutting medium (nutrient soil: garden soil: perlite: 3: 1) consisting of nutrient soil, garden soil and perlite and the vertical cutting mode are more favorable for improving the survival rate of the upright stems of the euscaphis konishii. Therefore, the upright stems of the reed heads of the amomum villosum can be effectively subjected to cutting propagation.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.

Claims (4)

1. A cutting propagation method for upright stems of rhizoma Phragmitis of amomum villosum is characterized by comprising the following steps:
s1, cutting: cutting the reed head upright stem of the amomum villosum as the cutting, placing the cutting in the concentration of 100--1Soaking in NAA treatment solution for 2 h;
s2, a cutting method: thoroughly watering a cutting medium by water, and vertically inserting the soaked cutting slips into the cutting medium, wherein the cutting medium is formed by mixing nutrient soil, garden soil and perlite;
s3, managing after insertion; the reed-head upright stem is collected from a two-year-old reed head and upright stems above the reed head; before the cutting shoot is treated, the lower end of the reed head part of the reed head upright stem and the upper end of the upright stem part are cut flat to form a cutting shoot with the length of 25-30 cm;
the mixing weight ratio of the nutrient soil, the garden soil and the perlite is 2-4:2-4: 1;
the vertical insertion depth of the reed-head upright stem is the buried reed-head part.
2. The cutting propagation method of the erect stem of the rhizoma Phragmitis of the amomum villosum as claimed in claim 1, wherein the concentration of the NAA treatment solution is 100-200 mg-L-1。
3. The cutting propagation method for the rhizoma Phragmitis erect-stem of the amomum villosum according to claim 1, wherein the cutting medium is subjected to solarization and disinfection in advance before cutting.
4. The use of the method for the cutting propagation of the reed rhizome upright stems of the amomum villosum according to any one of claims 1 to 3 in the propagation of the seedlings of the amomum villosum.
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