CN110915442B - Method for cutting and propagating root tips of rhizomes of amomum villosum - Google Patents
Method for cutting and propagating root tips of rhizomes of amomum villosum Download PDFInfo
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- CN110915442B CN110915442B CN201911174387.1A CN201911174387A CN110915442B CN 110915442 B CN110915442 B CN 110915442B CN 201911174387 A CN201911174387 A CN 201911174387A CN 110915442 B CN110915442 B CN 110915442B
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Abstract
The invention belongs to the technical field of plant propagation, and discloses a method for cutting and propagating the root-shaped stem tip of amomum villosum, which achieves the purpose of realizing seedling propagation by using materials of all parts of the amomum villosum plant through the selection of cutting ears and the selection of a cutting mode and the management after the cutting on the basis of utilizing a plant growth regulator. The cuttage material used by the method is light and small in occupied volume, and can effectively overcome a plurality of problems in transportation; meanwhile, the cutting material can come from the stems and the tips of the amomum villosum roots coming out from the garden, so that the waste is recycled. The cutting propagation can enrich the seedling raising means of the amomum villosum, solve the seedling problem of the amomum villosum, provide technical support for the rapid cutting propagation of the amomum villosum in future, and have positive significance for the production of the amomum villosum.
Description
Technical Field
The invention relates to the technical field of plant propagation, in particular to a method for cutting and propagating the stem tip of the rhizomes of amomum villosum.
Background
Yangchun sand (Yangchun sand)Amomum villosumLour.) is derived from Amomum genus (of Zingiberaceae family: (A. Merrill.) (L. etAmomumRoxb.) evergreen herbaceous plant, the part used as medicine is the dry and mature fruit, also called Chun Sha ren, which is one of the famous four southern medicines in China. Fructus amomi has the functions of resolving dampness and stimulating appetite, warming spleen and checking diarrhea, regulating qi and preventing miscarriage, is mainly used for treating qi stagnation of spleen and stomach, indigestion, abdominal pain and distension, is a traditional bulk common medicinal material in China, and has an application history of over 1300 years. At present, the Yangchun sand is mainly distributed in Guangdong, Guangxi, Yunnan and Fujian, etc., wherein the quality of Guangdong Yangchun is the best. Fructus amomi is a traditional bulk common medicinal material in China, has large market demand and is estimated to exceed 2.2 multiplied by 10 every year6kg, the market demand of the related industries exceeds 100 billion Yuan RMB. While the yield of the domestic fructus amomi is about 1.6 multiplied by 106kg, far from meeting the market demand. The main reasons for low yield include high fruit drop rate, low natural fruiting rate, high artificial pollination strength, etc. In addition, the field environment and germplasm type of amomum villosum can also affect the yield.
The source of the seedlings is a key production link, and the conventional fructus amomi breeding method mainly comprises two modes of seed breeding and plant division breeding at present. The defects of seed propagation are that the germination time of the seeds is long, the service life of the seeds is short, and the seeds are difficult to store, and the defect of the plant division propagation is that the propagation coefficient is low.
The cutting propagation is a propagation method for inserting the isolated organs of the plant such as roots, stems, leaves and the like into a matrix and utilizing the totipotency of plant cells to breed complete new plants under proper conditions. The cutting can be divided into stem cutting, root cutting and leaf cutting according to different cutting slips. The cutting propagation can maintain the excellent characteristics of the original variety, the seedling yield is high, the seedling forming speed is high, and the defect that part of plant species are difficult to sow and propagate is effectively overcome, so that the application in production is relatively wide. Factors for success or failure of plant cutting propagation mainly include selected cutting materials, endogenous hormones, nutrient substances, oxidase activity and the like.
The prior art has no report related to the cutting propagation of the amomum villosum.
Disclosure of Invention
Aiming at the technical defect that the prior art is lack of a research on a cutting propagation method of amomum villosum, the invention firstly provides a cutting propagation method of the root-shaped stem tip of the amomum villosum.
The second purpose of the invention is to provide the application of the sand rhizome stem tip cutting propagation method.
The purpose of the invention is realized by the following technical scheme:
the method for cutting propagation of the stem tip of the rhizomes of amomum villosum comprises the following steps:
s1, cutting selection: selecting a stem tip and a stem section which is 25-30 cm below the root of the spring sand growing in the current year;
s2, cutting treatment: soaking the whole root tip of the amomum villosum rhizome in 100-500 mg/L NAA;
s3, selecting a cutting medium: selecting nutrient soil, garden soil and perlite as a cutting medium, and pretreating the cutting medium;
s4, cutting: transversely burying the entire stem tip of the Yangchun sand rhizome treated by the S2 in the pretreated cutting medium of the S3;
and S5, managing after cuttage.
The most common propagation method of the amomum villosum is plant division propagation, namely whole stem and leaf are kept for field planting, but the amomum villosum required by the propagation method keeps whole stem and leaf, and the time required by the propagation method is not beneficial to quick propagation and yield improvement of the amomum villosum.
In the process of realizing cutting propagation of the amomum villosum, the inventor finds that on the basis of utilizing the plant growth regulator, the aim of realizing seedling propagation by utilizing materials of all parts of the amomum villosum plant can be achieved only by adopting specific requirements in each step from the selection of cutting slips and the selection of a cutting mode and then the management after cutting.
Preferably, the concentration of the NAA in S2 is 100-300 mg/L, so that the survival rate of the stem tip of the root-shaped stem of the amomum villosum can be further improved.
Preferably, the mixing ratio of the nutrient soil, the garden soil and the perlite in the cutting medium of S3 is 3:3: 1.
preferably, the amomum villosum rhizome tip is placed in water at S1, and the other end of the amomum villosum rhizome tip is cut to be flat, so that the cutting material is saved.
Preferably, the pretreatment of the cutting medium in S3 is: the cutting medium is solarized, disinfected and subpackaged, and then is watered thoroughly with tap water which is placed overnight.
Specifically, the sub-packaging is to sub-package the cutting medium into the plastic flower pot, and the specification of the plastic flower pot is as follows: length, width, height =50cm 15 cm.
Preferably, the depth of the whole root of the stem tip of the amomum villosum rhizome transversely buried is 2-3 cm in S4.
The invention also provides application of the amomum villosum rhizome tip cutting propagation method in improving the yield of the amomum villosum.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for cutting propagation of the stem tip of the rhizomes of amomum villosum, which is characterized in that on the basis of utilizing a plant growth regulator, the aim of realizing seedling propagation by utilizing materials of all parts of the amomum villosum plants is fulfilled by selecting cutting slips and a cutting mode and managing after cutting.
The cuttage material used by the method is light and small in occupied volume, and can effectively overcome a plurality of problems in transportation; meanwhile, the cutting material can come from the stems and the tips of the amomum villosum roots coming out from the garden, so that the waste is recycled. The cutting propagation can enrich the seedling raising means of the amomum villosum, solve the seedling problem of the amomum villosum, provide technical support for the rapid cutting propagation of the amomum villosum in future, and have positive significance for the production of the amomum villosum.
Drawings
FIG. 1 is a schematic view showing the shape and size of cutting slips of the stem tip of the root-shaped stem of amomum villosum;
FIG. 2 is a graph showing the effect of different NAA concentrations on the survival rate of the shoot tips of the rhizomes;
FIG. 3 is a graph showing the effect of different NAA concentrations on the rooting and budding rate of the shoot tips of the rhizomes;
FIG. 4 shows the rooting of the shoot tip of the rhizome;
FIG. 5 shows shoot emergence from the shoot tip of a rhizome;
fig. 6 shows the effect of different cutting medium formulations on the survival rate of the shoot tips of the rhizomes.
Detailed Description
For a more clear understanding of the technical features, objects and effects of the present invention, embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
1. Cutting materials:
the plant of YANGCHUNSHA is selected from YANGCHUNSHA shed of ZHEN SHAN at school district of university of traditional Chinese medicine, Guangzhou. The cutting is the stem tip of the rhizome, which is the stem tip of the current year and the stem section of 25-30 cm below, as shown in figure 1.
2. Reagent: alpha-naphthylacetic acid (NAA, Shanghai Boo Biotech Co., Ltd.: 070811).
3. The cuttage method comprises the following steps:
(1) and (3) cutting treatment: the cutting slips are placed in distilled water after being primarily trimmed, the stem tip of the rhizome is cut flat at the lower end, and the stem tip at the upper end is not required to be trimmed. The length of the treated cutting slips is 25-30 cm. Setting concentration gradients of 5 NAAs (total concentration of NAA) of 0, 100, 200, 300 and 500 mg.L < -1 >, and soaking the whole cutting in NAA treatment solutions with different concentrations for 2 h.
(2) The cuttage process comprises the following steps: the formula of the cutting medium is nutrient soil: garden soil: perlite is 3:3: 1. The substrate was sterilized by exposure to the sun, and then, it was distributed in plastic pots (length × width × height =50cm × 15cm × 15 cm), and was watered with tap water overnight. The whole root of the shoot tip of the cutting rhizome is transversely buried in a plastic long flowerpot with the depth of 2-3 cm, and 5 cutting shoots are buried in each pot. 2 pots were treated each in 1 group, and 3 groups were repeated.
(3) Managing after the insertion: the flowerpot is arranged in a sunshade to prevent direct sunlight; watering properly according to the humidity of the substrate to keep the substrate wet; timely weeding.
S3, selecting a cutting medium: selecting nutrient soil, garden soil and perlite as a cutting medium, and pretreating the cutting medium;
s4, cutting: transversely burying the entire stem tip of the Yangchun sand rhizome treated by the S2 in the pretreated cutting medium of the S3;
and S5, managing after cuttage.
4. Statistics of results
And observing the external form change of the cutting slips once a week after cutting, and counting the survival rate, the rooting rate, the budding rate and other growth indexes of the cutting slips after two months. Root diameter was measured with a vernier caliper at a root distance of 2 mm from the base. The experimental data are expressed as ` x. + -. s, and are counted and analyzed by using SPSS 25.0 software.
Firstly, the influence of NAA treatment with different concentrations on the cuttage propagation of the stem tip of the rhizomes of the amomum villosum
a. Influence of NAA treatment with different concentrations on survival rate of the stem tip of the rhizomes of amomum villosum
Respectively using concentration gradients of 0, 100, 200, 300, 500 mg.L-1The cutting slips are processed by NAA, the survival rate of the cutting slips is counted after 2 months of cutting, and the result is shown in figure 2. The results in figure 2 show that the survival rate of the cuttings after NAA treatment is higher than that of the control group, but the survival rate of the control group also reaches 83.33%. On the whole, the survival rate shows a parabolic trend from low to high to low, and the NAA concentration is 200 mg.L-1The survival rate reaches the highest point and is 100.00 percent. With the increase of the concentration of NAA, the survival rate of the cutting slips is reduced. The analysis reason is that the hormone maintenance mechanism of the plant can be broken by the NAA with too high concentration, so that the internal hormone level balance is disturbed, and the survival rate is influenced.
b. Influence of NAA treatment with different concentrations on rooting and budding rate of the stem tip of the rhizomes of the amomum villosum
To further examine the effect of different concentrations of NAA on the growth of the cuttings, statistics and analyses were performed on the rooting rate and the germination rate, and the results are shown in FIG. 3. Consistent with the survival rate trend, the rooting rate and the budding rate show the trend of increasing first and then decreasing along with the increase of the NAA concentration. The rooting rate and the budding rate of the stem tip of the rhizome are both 200 mg.L at the concentration of NAA-1The peak time is reached, and is 66.67 percent and 33.33 percent; the rooting rate of the treatment groups is greater than or equal to that of the control group; however, the germination rate of the treated group was 100 mg.L in NAA concentration-1Lower than the control, with a value of 0.
c. Influence of NAA with different concentrations on other growth indexes of the stem tip of the rhizome of amomum villosum
In order to more fully understand the effect of NAA treatment at different concentrations on the cuttings, other growth indicators were measured, including root number, root length, root diameter, etc., and the results are shown in Table 1. As shown in Table 1, the molecular weight distribution was 200 mg. L-1After NAA treatment, the average root number of the shoot tip of the rhizome reaches 2.79, the average root length is 5.2 cm, the longest root length is 8.80cm, and the average root length is obviously higher than that of other treatment groups, which indicates that the growth of the cutting shoot is optimal after NAA treatment at the concentration. The shoot tip of the cutting rhizome is shown in figure 4 and figure 5.
Note: the average followed by the same letter indicates no significant difference at the 0.05 level.
Secondly, the influence of the cuttage substrate proportion on the survival rate of the stem tips of the rhizomes of the amomum villosum
Respectively preparing cuttage matrixes with different proportions, and adopting the cuttage pretreatment and cuttage process (the NAA soaking concentration is 200 mg.L)-1) And counting the survival rate of the cutting slips after 2 months of cutting, wherein the result is shown in fig. 6 (in fig. 6, the abscissa represents the formula of the cutting medium, wherein the formula of the cutting medium of 1 is nutrient soil: garden soil: the formula of the cutting substrate of perlite which is 3:3:1, 2 is nutrient soil: garden soil: the formula of the cutting medium of perlite which is 1:3:1, 3 is that the nutrient soil: the formula of the cutting medium of perlite which is 1:1, 4 is nutrient soil: river sand: perlite is 3:3: 1). The results in fig. 6 show that the formula of the cutting medium has a remarkable effect on the survival rate of the cutting propagation of the stem tip of the rhizomes of amomum villosum.
Thirdly, the influence of the cuttage mode on the survival rate of the stem tips of the rhizomes of the amomum villosum
The experiment researches the influence of different cuttage modes on the survival rate of the stems and the tips of the rhizomes of the amomum villosum, and adopts the cuttage pretreatment and the cuttage process (the NAA soaking concentration is 200 mg.L)-1) The only difference is that the cutting mode is slightly different, the specific operation is as follows, and then the survival rate of the cutting slips is counted after 2 months of cutting.
A cuttage mode 1: the whole root of the shoot tip of the cutting rhizome is placed in a plastic long flowerpot, and the cutting mode is 2: the oblique (45-degree inclination angle) of the shoot tip of the cutting shoot rhizome is inserted into the cutting medium, the insertion depth is 1-2 cm, and the cutting mode is 3: the shoot tip of the cutting rhizome is transversely buried in a plastic long flowerpot in a depth of 2-3 cm; 5 cutting shoots are buried in each pot. 2 pots were treated each in 1 group, and 3 groups were repeated.
The results show that the survival rates of the stem tips of the villous amomum villosum rhizomes obtained by adopting the cutting modes 1, 2 and 3 are respectively 80%, 85% and 100%, which shows that the survival rate of the stem tips of the villous amomum villosum rhizomes is also obviously influenced by the cutting modes.
While the present invention has been described with reference to the embodiments shown in the drawings, the present invention is not limited to the embodiments, which are illustrative and not restrictive, and it will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (3)
1. The method for cutting propagation of the stem tip of the amomum villosum rhizome is characterized by comprising the following steps:
s1, cutting selection: selecting a stem tip and a stem section which is 25-30 cm below the root of the spring sand growing in the current year;
s2, cutting treatment: soaking the whole stem tip of the root-shaped stem of the amomum villosum in 200 mg/L NAA for 2 hours;
s3, selecting a cutting medium: selecting nutrient soil, garden soil and perlite as a cutting medium, and pretreating the cutting medium;
s4, cutting: transversely burying the entire stem tip of the Yangchun sand rhizome treated by the S2 in the pretreated cutting medium of the S3;
s5, managing after cuttage;
s3, mixing the nutrient soil, the garden soil and the perlite in the cutting medium according to a mixing ratio of 3:3: 1; the pretreatment of the cutting medium in S3 is as follows: solarizing and sterilizing the cutting matrix, subpackaging, and then thoroughly pouring with tap water placed overnight; s4, the whole root of the stem tip of the root-shaped stem of the amomum villosum is transversely buried by 2-3 cm.
2. The method for cutting propagation of the amomum villosum rhizome tip as recited in claim 1, wherein the amomum villosum rhizome tip is in water and the other end of the amomum villosum rhizome tip is cut flat at S1.
3. Use of the method for the cutting propagation of the stem tip of the root-shaped stem of amomum villosum according to any one of claims 1 to 2 for increasing the yield of amomum villosum.
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| CN102652483A (en) * | 2012-05-23 | 2012-09-05 | 张家港芳香生物科技有限公司 | Rosemary stem tip cutting propagation method |
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