CN108925263B

CN108925263B - Cuttage breeding method for Yannan pseudo-Shaosao

Info

Publication number: CN108925263B
Application number: CN201810794471.2A
Authority: CN
Inventors: 刘俊; 罗金环; 洪文君; 何书奋; 曾德华; 麦志通; 陈文昭
Original assignee: Sanya Forestry Science Research Institute
Current assignee: Sanya Forestry Science Research Institute
Priority date: 2018-07-19
Filing date: 2018-07-19
Publication date: 2021-03-30
Anticipated expiration: 2038-07-19
Also published as: CN108925263A

Abstract

The invention discloses a cuttage breeding method of Yannan pseudo-Shaosao, which comprises a series of management steps of test site condition selection, cutting of cutting slips, soil matrix treatment, hormone treatment, cuttage, later-period management and the like.

Description

Cuttage breeding method for Yannan pseudo-Shaosao

Technical Field

The invention relates to the technical field of cutting seedling raising, in particular to a method for cutting and breeding Yangnan pseudo-Shaosao.

Background

The Hainan pseudo-Shaosao (Paranephilum hainanensis) is a sapindaceae evergreen arbor tree species, is a special species in China and is distributed in the cliff county of the Hainan island. Because of the rare number of plants and narrow distribution area, the plants are listed as rare or endangered protected plants in II grades of China and important protected wild plants in China (the first batch), and are also listed as extremely dangerous species by IUCN. The Hainan pseudo-Shaosao is naturally, fruity and rare, and is difficult to update. The big trees and old trees are decreasing day by day and are in the border of extinction. The research of the Hainan Yangshao is focused on chemical components, community ecology and soil characteristics of fat-soluble and volatile parts of leaves, but the research of the breeding technology is not reported yet. The number of wild plants of the Yannan pseudo-Shaosao is very small (less than 100 plants), so that the population quantity of the Yannan pseudo-Shaosao is effectively enlarged, and the research on the propagation technology and the cultivation technology of the Yannan pseudo-Shaosao is especially important. The number of the seeds of the Yannan pseudo-Shaosao is very small, the germination rate of the seeds is low, and the large-scale seedling culture is difficult to achieve. Therefore, a research on the cutting propagation breeding technology of the Yannan pseudo-Shaosao is needed, and the method suitable for cutting seedling raising of the Yannan pseudo-Shaosao has important significance.

It is necessary to develop the research of cuttage breeding technology. The method provides scientific basis for the protection and sustainable utilization of the Yannan pseudo-Shaoshanzi through the measures of mother tree scion collection, soil matrix preparation, cutting treatment, cuttage and management.

Disclosure of Invention

In order to save the endangered species of the Yannan pseudo-Shaosao in time, expand the number of the Yannan pseudo-Shaosao population and solve the problems of small number of seeds, low germination rate and the like, the invention adopts the following technical scheme to realize the method: a method for cutting and breeding Yannan pseudo-Shaosao comprises the following steps:

selecting adult Hainan pseudo-Shaoshanzi with good growth vigor and natural distribution community as a test cutting, and mainly comprising the following steps:

(1) conditions of the test: a nursery garden where the cutting of the Hainan pseudo-Shaosao is located needs a shade shed, and the light transmittance of the shade shed is adjusted to be 50% -60% through a sunshade net; adjusting the temperature of the greenhouse to 25-35 ℃ by a greenhouse air conditioner and a sprinkling irrigation system;

(2) cutting the cutting slips: processing the cutting to make the cutting length 14cm-16cm and the diameter 0.1cm-0.3cm, cutting the upper end of the cutting flatly, cutting the lower end of the cutting obliquely, and then sterilizing the cutting;

(3) treating a soil matrix: the cutting land soil matrix is prepared by mixing a coconut coir matrix and perlite according to the weight ratio of 1: 1, mixing and preparing, and adopting 3 per mill potassium permanganate solution for disinfection;

(4) hormone treatment: inserting the lower end of the cutting into a double-gill-GGR growth regulator with the concentration of 400mg/L for soaking for 4 hours and then carrying out cuttage;

(5) cuttage: cutting seedling raising is carried out by adopting a nutrition cup, the specification of the nutrition cup is 16cm in diameter and 18cm in height, 10 cutting slips are cut in each nutrition cup, and seedling raising maintenance and daily management are carried out after cutting.

In the further scheme, the method comprises the following steps of,

wherein the growth regulator is a double-Gel-GGR solution prepared by dissolving the growth regulator in 70% alcohol.

In the further scheme, the method comprises the following steps of,

in the step (2), 1% of carbendazim is adopted to soak the cuttings for 15min, so that the effects of sterilization and disinfection are achieved.

In the further scheme, the method comprises the following steps of,

the soil matrix of the cutting land in the step (3) can also be garden soil and river sand according to the proportion of 1: 1, and then adopting 3 per mill potassium permanganate solution for disinfection.

In the further scheme, the method comprises the following steps of,

the method comprises the following steps:

(1) conditions of the test: a shade shed is needed for a nursery where the cutting of the Hainan pseudo-Shaosao is located, and the light transmittance of the shade shed is adjusted to be 55% through a sunshade net; adjusting the temperature of the greenhouse to 30 ℃ through a greenhouse air conditioner and a sprinkling irrigation system;

(2) cutting the cutting slips: processing the cutting to enable the cutting to be 15cm long and 0.2cm in diameter, cutting the upper end of the cutting to be flat, cutting the lower end of the cutting to be oblique, and then sterilizing the cutting;

Has the advantages that:

(1) the cuttage breeding method has simple operation and obvious effect through the mother tree scion collection, soil matrix preparation, cutting treatment, cuttage, management measures and the like, and provides scientific basis for the protection and sustainable utilization of the Yangtze Shaoshanzi;

(2) the cuttage breeding method of the invention enlarges the number of the Yannan pseudo-Shaosao seed population, and solves the difficulties of small seed number, low germination rate and the like.

Drawings

FIG. 1 is a comparison of germination rates of cuttings from treatment groups;

FIG. 2 shows the seedling growth performance of 400mg/L double Gill-GGR treated group in S1 soil matrix.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, specific embodiments thereof are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention can be embodied in many different forms than those herein described and many modifications may be made by one skilled in the art without departing from the spirit and scope of the invention.

Detailed description of the preferred embodiment 1

A method for cutting and breeding Yannan pseudo-Shaosao comprises the following steps:

(2) cutting the cutting slips: treating the cutting to make the cutting have a length of 14-16 cm and a diameter of 0.1-0.3 cm, cutting the upper end of the cutting, cutting the lower end of the cutting, and soaking the cutting in 1% carbendazim for 15min to achieve the effect of sterilization;

(3) treating a soil matrix: the cutting land soil matrix is prepared by mixing a coconut coir matrix and perlite according to the weight ratio of 1: 1, sterilizing by adopting 3 per mill potassium permanganate solution, and also can adopt garden soil and river sand according to the ratio of 1: 1, mixing and preparing, and then adopting 3 per mill potassium permanganate solution for disinfection;

(4) hormone treatment: inserting the lower end of the cutting into a growth regulator of a double-gill-GGR solution with the concentration of 400mg/L and prepared by dissolving in 70% alcohol, and cutting after soaking for 4 h;

Specific example 2

(2) cutting the cutting slips: treating the cutting to make the cutting length 15cm, diameter 0.2cm, cutting the upper end of the cutting horizontally, cutting the lower end obliquely, soaking the cutting with 1% carbendazim for 15min to achieve the effect of sterilization;

(4) hormone treatment: inserting the lower end of the cutting into a growth regulator of a double-gill-GGR solution with the concentration of 400mg/L and prepared by dissolving in 70% alcohol;

Data example 1

The test is carried out in a greenhouse of the third City forestry science research institute in Hainan province, and two cutting seedling culture media are adopted for cutting seedling culture:

(1) v, coconut husk substrate: v perlite 1: 1, mixing and preparing, and recording as S1;

(2) v, field soil: v river sand 2: 1 is mixed to prepare the product, is recorded as S2,

the soil matrix in the 2 steps is disinfected by 3 per mill of potassium permanganate solution, and 10 cutting shoots are planted in each nutrition cup in a cuttage mode.

(1) And (3) experimental design: after being soaked in 1% carbendazim for 15min, the Hainan pseudo-Shaosao cutting slips are treated by 2 hormones such as ABT rooting powder and double Jier-GGR with the concentrations of 50mg/L, 100mg/L, 200mg/L and 400mg/L for 4h, and then the cutting test is carried out. Deionized water treatment was used as a control. Each treatment group is provided with 3 repetitions, each repetition is provided with 90 cuttings, and the observation time is 30d of cutting.

(2) As a result: as shown in table 1 below:

TABLE 1 comparison of different substrates and growth regulators for the cutting germination rate of Yangshao, Hainan

Test results show that after 30d of cuttage, the germination rate of the S1 soil matrix treatment group is higher than that of the CK group, and the germination rate of the double-Gill-GGR treatment group with the concentration of 400mg/L is the highest and is 66.67%; along with the increase of the concentration of ABT rooting powder, the germination rate of the Hainan pseudo-Shaoshanzi cuttings is in a trend of increasing first and then decreasing; with the increase of the concentration of double gill-GGR, the germination rate of the double gill-GGR is in an increasing trend, except for the ABT rooting powder treatment group with the concentration of 50mg/L, the germination rate of cuttings of the S2 soil matrix treatment group is higher than that of the CK group, and the germination rate of the double gill-GGR treatment group with the concentration of 400mg/L is the highest and is 33.33%.

In a whole view, the germination rate of the inserted cutting of the Yangtze Shaoshanzi in the S1 soil matrix is higher than that of the S2 soil matrix, and the effect of the double-gill-GGR growth regulator on promoting the germination rate of the inserted cutting is better than that of ABR rooting powder; the highest germination rate was observed with 400mg/L double Gill-GGR in either S1 or S2 matrix.

Data example 2

The test was also carried out in the greenhouse of the third City of the Hainan province, forestry scientific research institute, and two cutting seedling raising media were used for cutting seedling raising:

(1) And (3) experimental design: after being soaked in 1% carbendazim for 15min, the Hainan pseudo-Shaosao cutting slips are treated by 2 hormones such as ABT rooting powder and double Jier-GGR with the concentrations of 200mg/L, 400mg/L and 400mg/L for 4h, and then the cutting test is carried out. Deionized water treatment was used as a control. Each treatment group is provided with 3 repetitions, each repetition is provided with 90 cuttings, and the observation time is cutting 90 d.

(2) As a result: as shown in table 1 below:

TABLE 2 comparison of different substrates and growth regulators for the rooting rate and rooting parameters of the cutting of Yangshao in Hainan province

From table 2, it can be seen that the experimental data show that after 90 days of cutting, different matrixes and different growth hormone concentrations have a great influence on the rooting rate of the cutting of the Yannan pseudo-Shaosao. In the S1 soil matrix, the rooting rate of the Yangtze Shaosao cutting is more than 1.33%, the treatment groups have ABT rooting powder with the concentration of 200mg/L, double Jier-GGR with the concentration of 200mg/L and double Jier-GGR with the concentration of 400mg/L, and the rooting rates of other treatment groups and the CK group are both 0 (other treatment group data are not shown in Table 2, the same is applied below). In the S2 soil matrix, the rooting rate of only the double Gill-GGR treatment group with the concentration of 400mg/L is 12.22%, and the rooting rates of other treatment groups and the CK group are 0.

From the comparison of rooting parameters, the average root length, the number of adventitious roots, and the longest root length of the 400mg/L bigill-GGR-treated group in the S1 soil matrix were maximized, and 5.81cm, 16, and 8.6cm, respectively. In the S1 soil matrix, the rooting parameters of the double Gill-GGR treatment group with the concentration of 400mg/L are obviously higher than those of other treatment groups, and the rooting parameters of the double Gill-GGR treatment group with the concentration of 200mg/L are the next time. In the S2 soil matrix, the rooting parameters of the 400mg/L double Gill-GGR treated group were slightly lower than those of the S1 soil matrix, which was 200mg/L ABT, 200mg/L and 400mg/L double Gill-GGR treated groups.

The root system effect index result shows that the root system effect index of a double-Gill-GGR treatment group with the concentration of 400mg/L in S1 soil matrix is the highest and is 85.21; the concentration of 400mg/L double Gill-GGR and the concentration of 200mg/L ABT times (26.70 and 19.76) are both significantly higher than the other treatment groups and the CK group. In S2 soil matrix, the root system effect index of the double Gill-GGR treatment group with the concentration of 400mg/L is also the highest and is 13.83. Reflecting that in the soil matrix of S1, the treatment with high concentration of double Jiel-GGR is beneficial to the rooting of the Hainan pseudo-Shaoshan cuttings.

The ABT and the double-Gill-GGR growth regulators have different composition contents and are soluble powder. 50% of total active ingredients of ABT, 20% of naphthylacetic acid and 30% of indoleacetic acid; the double Gill-GGR contains more than or equal to 20 percent of amino acid, more than or equal to 2 percent of trace element, less than or equal to 5mg/kg of limiting element Hg, less than or equal to 10mg/kg of As, less than or equal to 10mg/kg of Cd, less than or equal to 50mg/kg of Pb and less than or equal to 50mg/kg of Cr.

The cutting propagation method of the invention, which adopts the cutting ear picking of the mother tree, the soil matrix preparation, the cutting treatment, the cutting and management measures and the like, has simple operation and obvious effect, provides scientific basis for the protection and the sustainable utilization of the Hainan pseudo-Shaoshanzi, enlarges the population quantity of the Hainan pseudo-Shaoshanzi, and solves the difficulties of small seed quantity, low germination rate and the like.

While the invention has been described with reference to certain embodiments, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention, and in particular, features of various embodiments of the invention may be combined in any manner without departing from the technical conflict, the embodiments not expressly described in this disclosure being for the purpose of brevity and resource conservation. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed herein, but that the invention will include the claims appended hereto.

Claims

1. A Hainan pseudo-Shaosao cutting breeding method is characterized in that: selecting adult Hainan pseudo-Shaoshanzi with good growth vigor and natural distribution community as a test cutting, and mainly comprising the following steps:

(4) hormone treatment: inserting the lower end of the cutting into a double-gill-GGR solution prepared by dissolving 70% alcohol with the concentration of 400mg/L for soaking for 4 hours and then carrying out cuttage;

2. The Hainan pseudo-Shaosao cutting propagation method according to claim 1, wherein: in the step (2), 1% of carbendazim is adopted to soak the cuttings for 15min, so that the effects of sterilization and disinfection are achieved.

3. The Hainan pseudo-Shaosao cutting propagation method according to claim 1, wherein: the soil matrix of the cutting land in the step (3) can also be garden soil and river sand according to the proportion of 1: 1, and then adopting 3 per mill potassium permanganate solution for disinfection.

4. The Hainan pseudo-Shaosao cutting propagation method according to claim 1, wherein: the method comprises the following steps:

(4) hormone treatment: inserting the lower end of the cutting into a growth regulator with the concentration of 400mg/L bigill-GGR, soaking for 4h, and then carrying out cuttage;