CN104813771A - Method for promoting michelia sphaerantha seed germination - Google Patents
Method for promoting michelia sphaerantha seed germination Download PDFInfo
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- CN104813771A CN104813771A CN201510183191.4A CN201510183191A CN104813771A CN 104813771 A CN104813771 A CN 104813771A CN 201510183191 A CN201510183191 A CN 201510183191A CN 104813771 A CN104813771 A CN 104813771A
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Abstract
The invention relates to a method for promoting michelia sphaerantha seed germination. The method includes stratification, dense sulfuric acid treatment, sodium bicarbonate treatment, plant clastic enzyme treatment, 6-BA treatment, calcium chloride treatment and plant extract treatment. The germination rate of michelia sphaerantha seeds can be increased by 50%, germination uniformity can be remarkably improved, and the method has a wide application prospect and is of great significance for promoting cultivation of michelia sphaerantha new varieties and stock reproduction.
Description
Technical field
The present invention relates to the method for plant seed germination, be specifically related to a kind of method promoting M. sphaerantha seed germination.
Background technology
M. sphaerantha is Magnoliaceae Michelia aiphyllium, high 18-25 rice, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 80 centimetres, and bark taupe, in irregular thin lobe, has the hole skin of remarkable projection; Sprout is slightly very thin, and yellow green is glossy, old branch bottle green, has glaucous large hole skin and lengthwise striped.Leaf ground paper matter, long 12-22 (-28) centimetre, wide 5-8 (-10) centimetre, long ellipticity obovate or the shape of falling ovum lanceolar, the short gradually point of tip tool, the full edge of base portion wedge shape, the micro-tool gloss of surperficial bottle green; Back side celadon, tool yellowish-brown pubescence (cultivation fleece is rare); Master pulse and survey arteries and veins front side recess, master pulse be that the channel form petiole base that goes directly connects with stipule trace, and back side protuberance, lateral vein 8-13 couple, net arteries and veins is clear debates.The long 2-3 (-4.5) centimetre of petiole, stipule trace is less than 1/2nd of its length.The good species of M. sphaerantha chain timbers the orchid family.Grow faster, aiphyllium, tree crown carries out tree performance grace, and the flower that early summer is pure white is completely set, and autumn non-bunchiness erythrocarpus pendency is very beautiful.For good garden is viewed and admired and Shade Trees.
Along with the effect of M. sphaerantha in green industry becomes increasingly conspicuous, its utilization and rearing new variety are paid attention to day by day.And seminal propagation is the main method of cultivating new varieties and breeding stock.But M. sphaerantha seed coat is hard, have confinement effect to the absorption of moisture and respiratory metabolism, and pericarp, seed coat, in embryo all containing abscisic acid (ABA), have the characteristic of deep-sleep, therefore germination rate is general very low and irregular.For improving breeding efficiency and resource utilization, need to process seed, conventional method for treating seeds safety is lower, and treatment effect is undesirable.
The present invention is by Seed Germination Test, and the method adopting physics and chemistry process to combine, solves the problem that M. sphaerantha seed germination rate is low.
Summary of the invention
The present invention is directed to some problems in currently available technology, provide a kind of method promoting M. sphaerantha seed germination.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of method promoting M. sphaerantha seed germination, comprise the steps:
1), Stratificated treatment: M. sphaerantha seed is husky with wet, perlite mixes, and wherein wet husky water content is 50%, after plastic bag sealing, is placed in 4 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 1min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 3% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 3%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 150mg/L, 26 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 20mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, M. sphaerantha seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) is dry by axletree blade of grass, pulverizing obtains powder I, ethanol and powder I in mass ratio 6:1 ~ 8:1 mix, refluxing extraction 10 ~ 30 minutes, filter extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:200 ~ 1:500 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Calcium ion is the necessary mineral nutrient element of plant growth, is also a kind of important Metabolism regulation material.The interpolation of External Calcium can improve the content of soluble sugar in endosperm, can also improve the activity of amylase in Seed Germination, lipase, glutamic-pyruvic transaminase, and then promotes the sprouting of seed.
Plant catabolic enzyme is a kind of protein mixture with the decomposition function of organization such as plant cell wall, cell membrane, mainly comprises cellulolytic enzyme, pectin hydrolase etc.There is the performance of general protein, also there is the specificity as a kind of enzyme simultaneously.The inventive method can make M. sphaerantha percentage of seedgermination improve 50%, can significantly improve germination regularity simultaneously, have broad application prospects, for the breeding of the cultivation and stock that promote M. sphaerantha new varieties has great importance.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
The embodiment of the present invention comprises:
Embodiment 1
Promote the method for M. sphaerantha seed germination, comprise the steps:
1), Stratificated treatment: M. sphaerantha seed is husky with wet, perlite mixes, and wherein wet husky water content is 50%, after plastic bag sealing, is placed in 4 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 1min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 3% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 3%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 150mg/L, 26 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 20mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, M. sphaerantha seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) by dry for axletree blade of grass, pulverize and obtain powder I, ethanol and powder I in mass ratio 6:1 mix, refluxing extraction 10 minutes, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:200 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Embodiment 2
Promote the method for M. sphaerantha seed germination, comprise the steps:
1), Stratificated treatment: M. sphaerantha seed is husky with wet, perlite mixes, and wherein wet husky water content is 50%, after plastic bag sealing, is placed in 4 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 1min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 3% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 3%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 150mg/L, 26 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 20mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, M. sphaerantha seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) by dry for axletree blade of grass, pulverize and obtain powder I, ethanol and powder I in mass ratio 7:1 mix, refluxing extraction 20 minutes, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:300 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
Embodiment 3
Promote the method for M. sphaerantha seed germination, comprise the steps:
1), Stratificated treatment: M. sphaerantha seed is husky with wet, perlite mixes, and wherein wet husky water content is 50%, after plastic bag sealing, is placed in 4 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 1min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 3% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 3%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 150mg/L, 26 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 20mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
In described step 1, M. sphaerantha seed and wet sand, perlite mixed volume are than being 1:3:2.
The preparation method of described plant extraction liquid is: (1) by dry for axletree blade of grass, pulverize and obtain powder I, ethanol and powder I in mass ratio 8:1 mix, refluxing extraction 30 minutes, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:500 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
The seed germination rate of embodiment 1-3 reaches 80%, 85%, 83% respectively, relative to only carry out Stratificated treatment contrast seed 30%, improve about 50%, relative to only carried out step 1-6 contrast seed 50%, improve about 30%.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. promote a method for M. sphaerantha seed germination, it is characterized in that: comprise the steps:
1), Stratificated treatment: M. sphaerantha seed is husky with wet, perlite mixes, and wherein wet husky water content is 50%, after plastic bag sealing, is placed in 4 DEG C of refrigerator low temperature treatment, takes out and clean after 20d;
2), dense sulfuric acid treatment: by through step 1) seed after process is soaked in the concentrated sulfuric acid, process 1min, takes out and cleans;
3), sodium bicarbonate process: by through step 2) seed after process is soaked in the sodium bicarbonate solution of concentration 3% of 65 DEG C, constantly stir, process 20min, takes out and cleans;
4), the process of plant catabolic enzyme: by through step 3) seed after process is immersed in the plant catabolic enzyme solution of concentration 3%, 20 DEG C by day, night processes 40h under the room temperature condition of 10 DEG C, take out and clean;
5), 6-BA process: by through step 4) seed after process is soaked in the 6-BA solution of 150mg/L, 26 DEG C by day, night processes 24h under the room temperature condition of 13 DEG C, take out and clean;
6), calcium chloride process: by through step 5) seed after process is soaked in the calcium chloride solution of 20mmol/L, 20 DEG C by day, night processes 12h under the room temperature condition of 15 DEG C, take out and clean;
7), plant extraction liquid process: by through step 6) seed after process is soaked in plant extraction liquid, 27 DEG C by day, night processes 36h under the room temperature condition of 14 DEG C, take out and clean.
2. the method for claim 1, is characterized in that: in described step 1, M. sphaerantha seed and wet sand, perlite mixed volume are than being 1:3:2.
3. the method for claim 1, it is characterized in that: the preparation method of described plant extraction liquid is: (1) is dry by axletree blade of grass, pulverizing obtains powder I, ethanol and powder I in mass ratio 6:1 ~ 8:1 mix, refluxing extraction 10 ~ 30 minutes, filter extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:200 ~ 1:500 mix, and ultrasonic process 2 ~ 4 minutes, obtains solid content II suspension and plant extraction liquid.
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| CN201510183191.4A CN104813771A (en) | 2015-04-17 | 2015-04-17 | Method for promoting michelia sphaerantha seed germination |
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| CN201510183191.4A CN104813771A (en) | 2015-04-17 | 2015-04-17 | Method for promoting michelia sphaerantha seed germination |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593028A (en) * | 2017-10-23 | 2018-01-19 | 柳州雅瑞科技有限公司 | A kind of method for promoting Robinia pseudoacacia Seeds to sprout |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430657A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds |
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2015
- 2015-04-17 CN CN201510183191.4A patent/CN104813771A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430657A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds |
Non-Patent Citations (1)
| Title |
|---|
| 马小英等: "木兰科植物种子繁殖研究概况", 《种子》, vol. 28, no. 10, 25 October 2009 (2009-10-25), pages 54 - 57 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593028A (en) * | 2017-10-23 | 2018-01-19 | 柳州雅瑞科技有限公司 | A kind of method for promoting Robinia pseudoacacia Seeds to sprout |
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Application publication date: 20150805 |