CN103380699B - Method for cutting and seedling raising of cauliflower male sterility line cluster buds - Google Patents

Abstract

The invention discloses a method for cutting and seedling raising of cauliflower male sterility line cluster buds. The method comprises the following steps of selecting a plot with fertile soil and good terrain to plan a cutting seed bed, and adopting a seedling culture matrix and river sand as a cutting matrix; selecting the current-year semi-lignified cluster buds as cutting slips, performing disinfection, sterilization and promotion of root forming substance treatment and then inserting the cutting slips into the seed bed; performing cutting and then performing disinfection, sterilization and fertilizer application, controlling temperature in a greenhouse and performing conventional management; performing transplanting and heeling after rooting and seedling hardening of plants and planting the plants in a large land after the plants grow healthy and strong. The survival rate of the cauliflower cluster buds obtained by means of the method reaches to 82.5%, and the growing situation of the plants in the land is not obviously different from seedling of seeds.

Description

A kind of method of cauliflower male sterile line Multiple Buds cuttage and seedling culture

Technical field

The present invention relates to a kind of method of cuttage and seedling culture, be specifically related to a kind of method of cauliflower male sterile line Multiple Buds cuttage and seedling culture, belong to plant tissue culture field.

Background technology

Cauliflower is a mutation of Cruciferae Brassica genus brassica specie, and edible organs is bouquet, originates in Mediterranean, 19th century imported south China into, due to its tender succulence, delicious flavour, dark liking by consumers in general, occupies critical role in China's vegetable growing and year-round supply.

The method of cottage propagation is one of vegetative important technical of plant, have variability little, take root be easier to, the advantage such as survival rate is high, fringe bar abundance.The key of cottage propagation is two aspects: one is permeability, the water content control of medium of seedling bed; Two is that in microenvironment, air humidity and temperature control.In existing achievement in research and documents and materials, the water content of medium of seedling bed and permeability are mainly by adopting soil+husky (Liu Chengxiu etc., 2013, 41 (4): 1812-813, 1821, Agriculture of Anhui science), river sand (Cui Xiangdong etc., 2013 (05): 56-59, north gardening), peat soil+perlite (+vermiculite) (Huang Qinjun etc., 2013, 33 (1): 27-33, Southwest Forestry University's journal), 200510010446.3) etc. the peat composed of rotten mosses+sand (application number: material builds well-drained seedbed, also have and directly adopt clay to do seedbed (application number: 200810049352.0).And seedbed microenvironment air relative moisture mainly adopts automatic spraying system to control.These methods are applied to the fields such as veterinary antibiotics, flower gardening material, achievements in forest-tree seedling, traditional Chinese medicine more.

At present about the cottage propagation research of cauliflower has no report, but in laboratory, adopt the method for tissue cultures to carry out preservation more with report numerous soon.(the Tianjin agricultural science such as Xia little Di, 1995,1(2): 22), refined (the northwest Botany Gazette such as surely of stone, 1998,18(4): 477-482), (the TanJin Agricultural College journal such as Xu Duanxiang etc. (Fujian agriculture science and technology, 2006, (5): 32-34), Huang Junxuan, 2006,13(4): 21-23) all successfully establish cauliflower tissue culture technique system and apply.But tissue culture method, concerning general grass-roots unit or plantation peasant household, has high input, technical requirement is high, be difficult to realize, therefore need a kind of easy, effective method, solve the problem run in production.

Summary of the invention

the technical problem solved:for the problem of cauliflower male sterile line propagation difficulty, the invention provides a kind of method of cauliflower male sterile line Multiple Buds cuttage and seedling culture, simple.

technical scheme:the invention provides a kind of method of cauliflower male sterile line Multiple Buds cuttage and seedling culture, mainly comprise the following steps:

(1) adopt seedling medium and river sand to do cutting medium, mixing, be laid in surface, cuttage seedbed, thickness is 4 ~ 7cm;

(2) adopt double-deck canopy film Shading net covering seedbed, set up shade apart from 0.4 ~ 0.5m place, shed top;

(3) choose cauliflower Multiple Buds and do cuttings, the long 5 ~ 15cm of cuttings, after disinfection and hestening rooting mass treatment, insert on seedbed, insertion depth 3 ~ 5cm, density 5cm × 5cm;

(4) after cuttage, uniform sprinkling irrigates seedbed, and shed is put up polyethylene dripless film and sunshade net;

(5) manage the phase after cuttage, carry out sterilizing, fertilising to seedbed, temperature of shed controls at 18 ~ 28 DEG C, and humidity is not less than 80%; The cuttage rooting phase, temperature of shed controls at 20 ~ 28 DEG C, and humidity is not less than 85%;

(6) after cuttage rooting 2 ~ 5 days, reduce shed humidity to 60 ~ 80% gradually, (4000lx is greater than) when intensity of illumination is large, sunshade net is added a cover outside shed, when light is weak, (lower than 800lx) raises the polyethylene dripless film of shed, makes plant progressively reform of nature environment;

(7) plant takes root and carries out transplanting after hardening and heel in, and when robust growth, field planting is to land for growing field crops.

Described seedling medium is Pin Shi vegetable seedling substrate, and the volume ratio of seedling medium and river sand is 4:1; Described cauliflower Multiple Buds is the cauliflower Multiple Buds of giving birth to lignification or semi-lignified then; Described material of taking root is 1000 mg/LNAA+1000 mg/L IBA.

beneficial effect:(1) adopt special seedling substrate+river sand to build seedbed, there is the advantage of moisturizing, ventilation, simple, easy to operate.(2), in general production, Multiple Buds is not as material use, and the present invention adopts the method for Multiple Buds cottage propagation, Multiple Buds is converted to the plant producing upper application, turn waste into wealth, improve utilization ratio, seedling cost can be made to reduce by 30 ~ 50%, remarkable in economical benefits.(3) seedling obtained, with seedling and plantlet in vitro no significant difference, plant growing way is good, is easy to survive, and has important using value.

Embodiment

embodiment 1

(1) the planning cuttage seedbed, plot selecting fertile soil, physical features good, seedbed thing extends, the wide 1.3m of bed surface, long 5m, a little higher than earth's surface of bed surface, and bed soil rake is broken, loose smooth.Top layer, seedbed matrix is based on Pin Shi vegetable seedling substrate, and river sand is auxiliary, and its volume ratio is 4:1, mixing, and be laid in surface, seedbed, thickness 4 ~ 7cm, the matrix that both prepare has good gas permeability and moisture capacity;

(2) shed adopts reinforcing bar (or bamboo chip) to make, and it is highly 0.8m, wide 1.4m, long 5m.Cover double-layer polyethylene Anti-fogging Film above shed, setting up shade apart from about 0.4 ~ 0.5m place, shed top, shade is made up of irony support, iron wire and black sunshade net;

(3) at the beginning of 6 months, the cauliflower Multiple Buds choosing raw lignification (or semi-lignified) then does cuttings, the long 5 ~ 15cm of cuttings.Ready access upon use is answered in cutting, avoids dehydration as far as possible.Be first tpn liquid (the tpn wetting powder as commercially available) 800 ~ 1000 times of (multiple of dilution) sterilizings 5 minutes of 75% ~ 80% by mass percentage concentration, to pull in rear immersion 1000 mg/LNAA+1000 mg/L IBA mixed liquor 10 minutes out, then the cuttings handled well is inserted on ready-made seedbed, insertion depth 3 ~ 5cm, density 5cm × 5cm;

(4) should, with inserting with uniform sprinkling, moisture be made to soak into matrix, and and then cover bilayer film button canopy, also will covered with sunshade net thereon when sunny temperature is higher.After whole cuttage, film periphery wants compacting, avoids ventilative, with moisturizing;

(5) manage the phase after cuttage, use weekly tpn solution (the same) carry out sterilizing to seedbed and apply fertilizer, temperature of shed controls at 18 ~ 28 DEG C, and relative moisture controls more than 80%; The cuttage rooting phase, temperature of shed controls at 20 ~ 28 DEG C, and relative moisture controls more than 85%;

(6) after cuttage rooting 2 ~ 5 days, reduce shed humidity to 60 ~ 80% gradually, when intensity of illumination is large, outside shed, add a cover sunshade net, raise the polyethylene dripless film of shed when light is weak, to increase illumination.Cutting survival seedling is because root system is few, root layer is shallow, and drought-resistant ability is poor, so often will water according to soil moisture situation, long prosperous gradually with plant, the corresponding minimizing of irrigation times, now should progressively remove shed film, makes plant progressively reform of nature environmental condition;

(7) in 9 ~ October, first 20 days of field planting, cuttage plant from seedbed out, be transplanted to booth and heel in, when plant to be planted growth has 5 ~ 6 leaves, root system healthy and strong, field planting is to land for growing field crops;

Investigation finds, the cauliflower Multiple Buds survival rate 82.5% obtained by the method for cuttage, and the growing state of plant in field and seedling from seed are without significant difference.

embodiment 2

Heel in, resident time select in late November to early December, cuttage now and transplanting time partially more late than embodiment 1, all the other operations of seedling raising process are identical with embodiment 1.Transplanting time is late, then plant length is more healthy and stronger, can without heeling in, and direct field planting is to breeding field.

Claims (2)

1. a method for cauliflower male sterile line Multiple Buds cuttage and seedling culture, is characterized in that comprising following operating procedure:

(1) adopt seedling medium and river sand to do cutting medium, mixing, be laid in surface, cuttage seedbed, thickness is 4 ~ 7cm; Described seedling medium is Pin Shi vegetable seedling substrate, and the volume ratio of seedling medium and river sand is 4:1;

(2) adopt double-deck canopy film to cover seedbed, set up shade apart from 0.4 ~ 0.5m place, shed top;

(3) choose cauliflower Multiple Buds and do cuttings, the long 5 ~ 15cm of cuttings, after disinfection and hestening rooting mass treatment, insert on seedbed, insertion depth 3 ~ 5cm, density 5cm × 5cm; Described cauliflower Multiple Buds is the cauliflower Multiple Buds of giving birth to lignification or semi-lignified then;

(4) after cuttage, uniform sprinkling irrigates seedbed, and shed is put up polyethylene dripless film and sunshade net;

(5) manage the phase after cuttage, carry out sterilizing, fertilising to seedbed, temperature of shed controls at 18 ~ 28 DEG C, and humidity is not less than 80%; The cuttage rooting phase, temperature of shed controls at 20 ~ 28 DEG C, and humidity is not less than 85%;

(6) after cuttage rooting 2 ~ 5 days, reduce shed humidity to 60 ~ 80% gradually, when intensity of illumination is greater than 4000lx, outside shed, add a cover sunshade net, in intensity of illumination lower than the polyethylene dripless film raising shed during 800lx, make plant progressively reform of nature environment;

(7) plant takes root and carries out transplanting after hardening and heel in, and when robust growth, field planting is to land for growing field crops.

2. the method for a kind of cauliflower male sterile line Multiple Buds cuttage and seedling culture according to claim 1, material of taking root described in it is characterized in that is 1000 mg/L NAA+1000 mg/L IBA.