CN107258547A - A kind of method of cauliflower Multiple Buds regeneration plant and application - Google Patents

A kind of method of cauliflower Multiple Buds regeneration plant and application Download PDF

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Publication number
CN107258547A
CN107258547A CN201710689446.3A CN201710689446A CN107258547A CN 107258547 A CN107258547 A CN 107258547A CN 201710689446 A CN201710689446 A CN 201710689446A CN 107258547 A CN107258547 A CN 107258547A
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China
Prior art keywords
cauliflower
multiple buds
greenhouse
plant
regeneration plant
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CN201710689446.3A
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Chinese (zh)
Inventor
牛国保
姚星伟
单晓政
孙德岭
刘莉莉
文正华
江汉民
张小丽
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Kerun Agricultural Science & Technology Co Ltd Tianjin
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Kerun Agricultural Science & Technology Co Ltd Tianjin
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Priority to CN201710689446.3A priority Critical patent/CN107258547A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers

Abstract

The invention discloses a kind of method of cauliflower Multiple Buds regeneration plant, comprise the following steps:The complete healthy and strong Multiple Buds of estrangedization then are chosen as regrowth, select the side sprouted in root to give birth to branch;It is regeneration seedbed to select flowerpot, and selection vermiculite and sieving fine earth are matrix;To lateral bud sterilization, HORMONE TREATMENT;Sterilization, fertilising, humidity, the management of intensity of illumination after definite value;Regeneration plant survive after field management.The present invention further discloses application of the cauliflower Multiple Buds regeneration plant method in terms of seedling percent is improved.Experimental result is shown:The cauliflower Multiple Buds survival rate obtained by the method for the present invention is up to 85%, plant field growing state well with seedling from seed without significant difference, the problem of Multiple Buds are not as material use is efficiently solved, cauliflower Multiple Buds utilization ratio is improved.

Description

A kind of method of cauliflower Multiple Buds regeneration plant and application
Technical field
The present invention relates to a kind of method of cauliflower regeneration plant, and in particular to a kind of cauliflower Multiple Buds regeneration plant Method and application, belong to plant tissue culture technical field.
Background technology
Cauliflower is that, using bouquet as product mutation, a biennial herb is planted in Cruciferae Brassica genus brassica specie Thing.By wild cabbage develops, evolution center is littoral in Mediterranean east.Incoming China in the middle of the 19th century.Because its is nutritious, Quality is tender, delicious flavour is liked by consumers in general deeply, occupies critical role in China's vegetable growing and year-round supply.
Multiple Buds breeding method be one of important technical of plant vegetative propagation, with variability it is small, take root compared with Easily, the advantages of survival rate height, abundance.But Multiple Buds consume plant nutrition as unfavorable character in cauliflower production, The growth of bouquet is influenceed, it is necessary to remove in time, is not as material use.
Multiple Buds refer to the colony's crowd shoots for developing to come from cauliflower foundation portion, are that many cells are formed mostly, bipolar Property, structural integrity can be formed directly in plantlet.The research about the Multiple Buds regeneration plant of cauliflower has no report at present, so And preserve more with fast numerous report using the method progress of tissue cultures in laboratory.Wan Qionglian etc.(Yunnan Prov Agriculture University Journal, 2016,31(1):49-53), Hu Limin etc.(Agricultural science and technology is communicated, and 2015,(6):284-286), Wu Lifang etc.(The north Gardening, 2009,(6):57-58)It has been successfully established cauliflower tissue culture technique system and has been applied.However, tissue training The cycle of supporting length, restrictive condition is more, input is big, technical requirements are high, and bouquet is rotten after plant is susceptible does not have utilizable tissue culture Material, plant regeneration is difficult to, the method for the Multiple Buds regeneration plant of cauliflower, is a kind of easy-to-use method, material The advantages of abundance, survival rate are high, variability is small, the problem of effectively solving to run into production, is cauliflower regeneration plant Important means.
The content of the invention
To solve in the cauliflower Breeding Process that presently, there are, the bouquet infection sclerotium that parent material runs into breeding Disease, soft rot etc., bouquet rot to reserve seed for planting, and cause the problem of parent material is lost.
The invention discloses a kind of method of cauliflower Multiple Buds regeneration plant, it is characterised in that enters by the steps OK:
(1)Using vermiculite and sieving fine earth as matrix, by volume 1:3 are well mixed, in the flowerpot for containing bore 35cm, base Matter thickness is the 2/3 of height of flowerpot;
(2)Use greenhouse for culture environment, the sunshade net of 60 mesh is hidden outside greenhouse;
93 choose the healthy and strong root Multiple Buds of cauliflower, highly 5~15cm, and disinfection is after the hestening rooting liquid prepared Culture medium dim light culture 16h;Described hestening rooting fluid nutrient medium refers to 20 mg/L NAA+600 mg/L IBA;
(3)It is colonized after water planting in flowerpot, 4 plants is colonized per basin, depth is 2cm, and uniform sprinkling is irrigated, and sunshade is put up on greenhouse Net;
(4)The phase is managed after field planting, sterilizing is carried out to flowerpot, is applied fertilizer, temperature control is in 20~25 DEG C, humidity in greenhouse It is not less than 70%~80%;Field planting is taken root the phase, and temperature control is at 15~25 DEG C in greenhouse, and humidity is not less than 80%~90% ;
(5)3~10 days after field planting, gradually reduce humidity in shed and, to 60~80%, be more than 4500lx in intensity of illumination When, sunshade net is capped outside greenhouse, the sunshade net in greenhouse is raised when intensity of illumination is less than 1500lx, plant is progressively adapted to Natural environment.
Cauliflower Multiple Buds of the present invention are the height 5-10cm given birth to then cauliflower root Multiple Buds.The sterilization Agent is 75% alcohol, spray sterilization 5 minutes.
The NAA that the present invention is used is naphthoic acid (1-Naphthylacetic acid) abbreviation, is a kind of organic compound, It is a kind of colorless solid for being soluble in organic solvent.It is the auxin analog in plant growth regulator, is usually used in commercialization Root of hair powder or rooting agent in.IBA is indolebutyric acid Indole-3-Butytric acid abbreviation, is a kind of organic compound Thing, can promote plant main root to grow, and improve germination percentage, survival rate.Two kinds of raw materials are commercially available.
The present invention further discloses application of the cauliflower Multiple Buds regeneration plant method in terms of seedling percent is improved. Experimental result is shown:The cauliflower Multiple Buds survival rate obtained by the method for the present invention is up to 85%, life of the plant in field Long situation, without significant difference, efficiently solves the problem of Multiple Buds are not as material use, improves flower with seedling from seed Cabbage Multiple Buds utilization ratio.
The survival rate that is run into the experiment for cauliflower Multiple Buds regeneration plant of the present invention is low, do not take root, stem under soil The problems such as perishable, plant is dehydrated dead, has successively carried out different culture media with research, the hormon concentration for comparing survival rate With the experiments such as the influence of the Multiple Buds influence, environment taken root to seedling percent after field planting are compared, emphasis is solved after field planting Take root difficulty, plant dehydration is dead, the problem of planting percent is low.
The successful key of method of the present invention is that matrix used has the advantages that moisturizing, ventilation, simple and easy to apply, operation side Just, it is economical and practical;Hormone fluid nutrient medium used, which can be effectively facilitated, takes root, and has had micro capillary root to generate in field planting, has had The survival rate improved after Multiple Buds field planting of effect;Management after Multiple Buds field planting, it is ensured that natural environment needed for seedling slow seedling.
The good effect that the method for cauliflower Multiple Buds regeneration plant disclosed by the invention has is:
(1)Vermiculite+sieving fine earth is used for matrix, there is moisturizing, ventilation, it is simple and easy to apply, it is easy to operate, it is economical real With.
(2)In general production, Multiple Buds are as unfavorable character, consumption plant nutrition, it is necessary to which removal in time, is not as material Material is utilized, and the present invention is converted into Multiple Buds to produce the plant of upper application using the method for Multiple Buds rooting propagation, change give up into Treasured, improves utilization ratio, solves the problem of cauliflower parent is had no harvest in reproductive process after disease occurs, cauliflower is numerous Bouquet is caused to rot during the disease such as infection sclerotiniose, soft rot during kind, difficulty of branching out, it is impossible to bloom and reserve seed for planting makes parent's material Material is lost, and suffers heavy losses.
(3)The seedling of acquisition and seedling and tissue-cultured seedling no significant difference, plant growing way are good, it is easy to survive, operating method It is simple and easy to apply, it is with low cost, with important application value.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and consumption or change Belong to protection scope of the present invention.Raw materials used and reagent of the invention is commercially available.
Embodiment 1
(1)Select the plot sieving fine earth and vermiculite of the non-crop in cruciferae of fertile soil first crop crop by volume:3;1 matches somebody with somebody Than being well mixed, uniformly spreading out 15cm in sun-drenched weather dries 3 days, and the matrix that both are prepared has well saturating Gas and water-retaining property, the matrix prepared are contained in the flowerpot that bore is 35cm, and thickness is the 2/3 of height of flowerpot;
(2)Greenhouse is made of reinforcing bar, and its height is 4m, wide 9m, long 60m.Polyethylene dripless film, film are covered above greenhouse The mesh sunshade net of overlying 60;
(3)At the beginning of 12 months, cauliflower root Multiple Buds then, height 5cm are chosen.Ready access upon use is answered, dehydration is avoided as far as possible. First sterilizing is sprayed with mass percentage concentration 5 minutes for 75% alcohol, 20 mg/L NAA+600 mg/ are immersed after pulling out 16h in L IBA fluid nutrient mediums, then side shoot field planting is handled well in ready-made flowerpot, insertion depth 2-3cm is equal per basin Even 4 plants of field planting;
(4)Moisture should be made to be impregnated with matrix with field planting with uniform sprinkling, 60 are covered thereon when sunny temperature is higher Mesh sunshade net;
(5)The phase is managed after field planting, sterilizing is carried out to flowerpot, is applied fertilizer, temperature control is at 20 DEG C in greenhouse, and humidity is not less than 70% ;Field planting is taken root the phase, and temperature control is at 15 DEG C in greenhouse, and humidity is not less than 80%;
(6)It is colonized after taking root 3 days, warm indoor humidity is gradually reduced to 60%, when intensity of illumination is more than 4500lx, in greenhouse Outer capping sunshade net, the sunshade net in greenhouse is raised when intensity of illumination is less than 1500lx, makes plant progressively reform of nature environment;
(7)Live seedling is colonized into because root system is few, root layer is shallow, drought-resistant ability is poor, so often to be watered according to soil moisture situation, Gradually grown with plant prosperous, irrigation times are accordingly reduced;
(8)In 1-2 months, 30 days after field planting, plant strain growth has 5~6 leaves, root systems gradually healthy and strong;
Investigation find, the cauliflower Multiple Buds survival rate 85% obtained by this method, plant field growing state with Seedling from seed is without significant difference.
Embodiment 2
(1)Select the plot sieving fine earth and vermiculite of the non-crop in cruciferae of fertile soil first crop crop by volume:3;1 matches somebody with somebody Than being well mixed, uniformly spreading out 15cm in sun-drenched weather dries 3 days, and the matrix that both are prepared has well saturating Gas and water-retaining property, the matrix prepared are contained in the flowerpot that bore is 35cm, and thickness is the 2/3 of height of flowerpot;
(2)Greenhouse is made of reinforcing bar, and its height is 4m, wide 9m, long 60m.Polyethylene dripless film, film are covered above greenhouse The mesh sunshade net of overlying 60;
(3)At the beginning of 12 months, cauliflower root Multiple Buds then, height 15cm are chosen.Ready access upon use is answered, dehydration is avoided as far as possible. First sterilizing is sprayed with mass percentage concentration 5 minutes for 75% alcohol, 20 mg/L NAA+600 mg/ are immersed after pulling out 16h in L IBA fluid nutrient mediums, then side shoot field planting is handled well in ready-made flowerpot, insertion depth 2-3cm is equal per basin Even 4 plants of field planting;
(4)Moisture should be made to be impregnated with matrix with field planting with uniform sprinkling, 60 are covered thereon when sunny temperature is higher Mesh sunshade net;
(8)The phase is managed after field planting, sterilizing is carried out to flowerpot, is applied fertilizer, temperature control is at 25 DEG C in greenhouse, and humidity is not less than 70% ;Field planting is taken root the phase, and temperature control is at 25 DEG C in greenhouse, and humidity is not less than 80%;
(9)It is colonized after taking root 10 days, warm indoor humidity is gradually reduced to 80%, when intensity of illumination is more than 4500lx, in temperature Outdoor capping sunshade net, the sunshade net in greenhouse is raised when intensity of illumination is less than 1500lx, makes plant progressively reform of nature ring Border;
(10)Live seedling is colonized into because root system is few, root layer is shallow, drought-resistant ability is poor, so often to be poured according to soil moisture situation Water, gradually grows prosperous with plant, and irrigation times are accordingly reduced;
(8)In 1-2 months, 30 days after field planting, plant strain growth has 5~6 leaves, root systems gradually healthy and strong;
Investigation find, the cauliflower Multiple Buds survival rate 85% obtained by this method, plant field growing state with Seedling from seed is without significant difference.
Embodiment 3
Resident time was selected in the first tenday period of a month in the last ten-days period in April to May, and resident time now is partially more late than embodiment 1, regenerative process Remaining operation and embodiment 1 it is identical.Transplanting time evening, then plant length is more healthy and stronger, can be without being colonized in greenhouse, can be with Directly open country is arrived in field planting.

Claims (4)

1. a kind of method of cauliflower Multiple Buds regeneration plant, it is characterised in that carried out by the steps:
Using vermiculite and sieving fine earth as matrix, by volume 1:3 are well mixed, in the flowerpot for containing bore 35cm, matrix Thickness is the 2/3 of height of flowerpot;
Use greenhouse for culture environment, the sunshade net of 60 mesh is hidden outside greenhouse;
The healthy and strong root Multiple Buds of cauliflower are chosen, 5~15cm of height, disinfection is trained after the hestening rooting liquid prepared Support base dim light culture 16h;Described hestening rooting fluid nutrient medium is referred to:20 mg/L NAA+600 mg/L IBA;
It is colonized after water planting in flowerpot, 4 plants is colonized per basin, depth is 2cm, and uniform sprinkling is irrigated, and sunshade net is put up on greenhouse;
The phase is managed after field planting, sterilizing is carried out to flowerpot, is applied fertilizer, temperature control is in 20~25 DEG C, humidity 70% in greenhouse ~80%;Field planting is taken root the phase, and temperature control is in 10~25 DEG C, humidity 80%~90% in greenhouse;
3~10 days after field planting, warm indoor humidity is gradually reduced to 60~70%, when intensity of illumination is more than 4500lx, Sunshade net is capped outside greenhouse, the sunshade net in greenhouse is raised when intensity of illumination is less than 1500lx, plant is progressively adapted to certainly Right environment.
2. the method for the cauliflower Multiple Buds regeneration plant described in claim 1, it is characterized in that the cauliflower Multiple Buds are The height 5-10cm given birth to then cauliflower root Multiple Buds.
3. the method for the cauliflower Multiple Buds regeneration plant described in claim 1, it is characterized in that the disinfectant is 75% wine Essence, spray sterilization 5 minutes.
4. application of the cauliflower Multiple Buds regeneration plant method described in claim 1 in terms of seedling percent is improved.
CN201710689446.3A 2017-08-14 2017-08-14 A kind of method of cauliflower Multiple Buds regeneration plant and application Pending CN107258547A (en)

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