CN103975835B - A kind of breeding method being rich in sulforaphen broccoli bud - Google Patents
A kind of breeding method being rich in sulforaphen broccoli bud Download PDFInfo
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- CN103975835B CN103975835B CN201410209381.4A CN201410209381A CN103975835B CN 103975835 B CN103975835 B CN 103975835B CN 201410209381 A CN201410209381 A CN 201410209381A CN 103975835 B CN103975835 B CN 103975835B
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Abstract
The invention discloses a kind of breeding method being rich in sulforaphen broccoli bud, feature is the extraction comprising expense dish crude flavonoid powder, the step of the preparation of self-control culture fluid, the selection of broccoli seeds and the step of process, the sprouting of broccoli seeds is until the step that shows money or valuables one carries unintentionally of broccoli seeds, finally the broccoli seeds showed money or valuables one carries unintentionally is placed on the gauze soaked with self-control culture fluid, at certain illumination condition, temperature is 20 ~ 25 DEG C, humidity is cultivate under the environment of 80 ~ 90%, and sprayed self-control culture fluid every 24 hours, until broccoli bud grows to 8 ~ 10mm or broccoli root 10 ~ 12mm can gather, obtain being rich in sulforaphen broccoli bud, advantage is that to have anti-corrosion property strong, output is high, sulforaphen content is high and breeding method is simple, instant effect.
Description
Technical field
The present invention relates to a kind of breeding method of broccoli bud, especially relate to a kind of breeding method being rich in sulforaphen broccoli bud.
Background technology
Sulforaphen is a kind of multifunctional enzyme inducer, body can be induced to produce II type detoxication enzyme-glutathione transferase (GST), quinone reductase (QR), with suspect carcinogen matter in this enzyme energy, prevent the hereditary factor in carcinogen destruction healthy cell, thus play antitumaous effect.
Broccoli has another name called broccoli, broccoli, sprouting broccoli etc., belongs to a mutation of Cruciferae Brassica genus brassica specie, is one, biennial herb plant, and originate in Italy, about 19 beginnings of the century were introduced into China.Nutrient component in broccoli, not only content is high, and it is very comprehensive, sulforaphen content shelter wherein has first of vegetables, the once anticancer Vegetable Research result of British scientist at the beginning of 1997 shows, auxiliary compounds containing Glucosinolate class in broccoli, has decomposited sulforaphen, and from broccoli, has isolated the DNA material with sulforaphen growth hormone gene from Glucosinolate.In broccoli, the content of sulforaphen is lower, be difficult to realize suitability for industrialized production, and the domestic research to broccoli at present mainly concentrates on the aspect such as preservation and freshness, breed breeding of broccoli, but the research for sulforaphen in broccoli etc. with the enrichment of physiological activator and strengthening aspect is less, the key therefore finding a kind of method improving sulforaphen content in broccoli to become broccoli resource to develop further.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind ofly has that anti-corrosion property is strong, output is high, sulforaphen content is high and breeding method is simple, the breeding method being rich in sulforaphen broccoli bud of instant effect.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of breeding method being rich in sulforaphen broccoli bud, comprises the following steps:
(1) selection of broccoli seeds and process
Select when produce per year receive, without go mouldy and breakage, free from admixture, full grains broccoli seeds, the broccoli seeds of select is placed in mass concentration be 7 ~ 9% the expense dish crude flavonoid powder aqueous solution immersion 8 ~ 12 hours after, rinse 3 times with clear water;
(2) sprouting of broccoli seeds
Broccoli seeds after cleaning is placed in glass container or plastic containers, after adopting the multilayer gauze soaked through clear water to be covered by broccoli seeds, broccoli seeds is placed in temperature is 25 ~ 30 DEG C, humidity is carry out cultivation rudiment until broccoli seeds shows money or valuables one carries unintentionally under the environment of 80 ~ 90%;
(3) cultivation of sulforaphen broccoli bud is rich in
The broccoli seeds showed money or valuables one carries unintentionally is placed on the gauze soaked with self-control culture fluid, at certain illumination condition, temperature is 20 ~ 25 DEG C, humidity is cultivate under the environment of 80 ~ 90%, and sprayed self-control culture fluid every 24 hours, until broccoli bud grows to 8 ~ 10mm or broccoli root 10 ~ 12mm can gather, obtain being rich in sulforaphen broccoli bud.
Described expense dish crude flavonoid powder preparation method is as follows: pulverized with medicinal cracker after 24 ~ 36 hours in 70 ~ 80 DEG C of dryings and cross 80 mesh sieves by fresh expense dish and obtain expense dish powder, to take after dish powder mixes in the ratio of solid-liquid ratio 1:50 with 30% ethanolic solution, lixiviate 4 ~ 5 hours at 50 ~ 60 DEG C, again under 3000r/min rotating speed after centrifugal 10min, obtain expense dish crude flavonoid powder leaching liquor after getting supernatant vacuum filtration, the freeze drying of expense dish crude flavonoid powder leaching liquor is obtained expense dish crude flavonoid powder.
Described self-control culture fluid raw material and parts by weight as follows:
Sucrose 0.3 part
Glucose 0.3 part
Mannose 0.1 part
MS medium (not containing agar and sucrose) 0.3 part
Take dish crude flavonoid powder 3.0 parts
Distilled water 96.0 parts.
Step (3) illumination condition is as follows for arranging:
00:00 ~ 06:00 is dark
06:00 ~ 12:00 white light
12:00 ~ 12:05 UV-C or UV-A
12:05 ~ 18:00 white light
18:00 ~ 24:00 is dark.
Compared with prior art, the invention has the advantages that: the invention discloses a kind of breeding method being rich in sulforaphen broccoli bud, comprise the extraction of expense dish crude flavonoid powder, the preparation of culture fluid, the selection of broccoli seeds and process, the sprouting of broccoli seeds, be rich in the cultivation of sulforaphen broccoli bud, expense dish crude flavonoid powder is adopted to have the erosion that certain bacteriostasis effectively can prevent bacterium and putrefying bacteria in broccoli bud cultivating process to the process of broccoli seeds, and take the germination that dish crude flavonoid powder can stimulate broccoli seeds, be conducive to the germination rate improving seed.
Modern study shows: the secondary metabolite-4-methyl sulphur oxygen butane glucosinolate of broccoli bud is the precursor substance that sulforaphen generates, glucose, sucrose, mannose may facilitate the secondary metabolism of broccoli bud, and the amount of 4-methyl sulphur oxygen butane glucosinolate in broccoli bud is increased; The conditions such as abiotic environment illumination in addition, temperature, moisture can affect the metabolism of glucorphanin in broccoli bud.Therefore, the culture fluid containing certain proportion glucose, sucrose, mannose and illumination, damp condition can improve the content of sulforaphen in broccoli bud effectively.
The broccoli bud sulforaphen assay result that commonsense method (MS medium+lucifuge) is cultivated and the present invention cultivates is as following table:
| Bud long (mm) | 0 | 2 | 4 | 6 | 8 | 10 |
| Control group (mg/g) | 0.23±0.06 | 0.62±0.08 | 1.36±0.11 | 1.78±0.09 | 3.52±0.17 | 3.48±0.12 |
| Processed group (mg/g) | 0.26±0.02 | 0.92±0.12* | 1.94±0.07** | 3.29±0.13** | 5.86±0.16** | 5.57±0.05** |
Note: * and control group have significant difference, and * * and control group have pole significant difference.
As seen from the above table: the sulforaphen content in broccoli bud raises gradually along with the long increase of bud, in broccoli bud, the content of sulforaphen slightly reduces and when bud is grown up in 8mm; Compared with control group, the sulforaphen content in processed group broccoli bud has significance to raise, and the longer significance of broccoli bud is stronger, and in bud length is for processed group broccoli bud during 8mm, sulforaphen content can improve 60.06% relative to control group.
Accompanying drawing explanation
Fig. 1 is the breeding method flow chart that sulforaphen broccoli bud is rich in the present invention;
Fig. 2 is sulforaphen comparision contents figure in broccoli bud in control group and processed group.
Embodiment
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
One, specific embodiment
Embodiment 1
Be rich in a breeding method for sulforaphen broccoli bud, comprise following concrete steps:
1, the selection of broccoli seeds and process
Select when produce per year receive, without go mouldy and breakage, free from admixture, full grains broccoli seeds be placed in mass concentration be 7 ~ 9% the expense dish crude flavonoid powder aqueous solution immersion 10 hours after rinse 3 times with clear water;
The preparation method of wherein taking dish crude flavonoid powder is as follows: pulverized with medicinal cracker after 30 hours 75 DEG C of dryings by fresh expense dish and cross 80 mesh sieves, to dish powder and 30% ethanolic solution be taken according to lixiviate 4.5 hours at 55 DEG C after the solid-liquid ratio mixing of 1:50, then after above-mentioned leaching liquor being got supernatant vacuum filtration after centrifugal 10min under 3000r/min rotating speed, obtain expense dish flavones leaching liquor, finally leaching liquor freeze drying is obtained expense dish crude flavonoid powder;
2, the sprouting of broccoli seeds
Broccoli seeds after cleaning is placed in glass container or plastic containers, with the multilayer gauze soaked through clear water, broccoli seeds covered, be placed on 28 DEG C, carry out under the environment of 85% humidity cultivating, rudiment is until broccoli seeds shows money or valuables one carries unintentionally;
3, the cultivation of sulforaphen broccoli bud is rich in
The broccoli seeds showed money or valuables one carries unintentionally is placed on the gauze soaked with self-control culture fluid, at certain illumination condition, temperature is 22.5 DEG C, humidity is cultivate under the environment of 85%, and sprayed self-control culture fluid every 24 hours, until broccoli bud grows to 8 ~ 10mm or broccoli root 10 ~ 12mm can gather, obtain being rich in sulforaphen broccoli bud.
The preparation method wherein making culture fluid by oneself is as follows: sucrose, glucose, mannose, MS medium (not containing agar and sucrose), expense dish crude flavonoid powder and distilled water are mixed by following weight fraction: sucrose 0.3 part, glucose 0.3 part, mannose 0.1 part, MS medium (not containing agar and sucrose) 0.3 part, take dish crude flavonoid powder 3.0 parts, distilled water 96.0 parts;
Illumination condition is as follows for arranging:
00:00 ~ 06:00 is dark
06:00 ~ 12:00 white light
12:00 ~ 12:05 UV-C or UV-A
12:05 ~ 18:00 white light
18:00 ~ 24:00 is dark.
In the broccoli bud that this implementation method is cultivated, sulforaphen content is 5.81mg/g.
Embodiment 2
Basic with above-described embodiment 1, its difference is:
1) selection of broccoli seeds and process: by select broccoli seeds be placed in mass concentration be 7% the expense dish crude flavonoid powder aqueous solution soak 12 hours; The fresh dish that takes to be pulverized with medicinal cracker after 36 hours 70 DEG C of dryings and crossed 80 mesh sieves, then will to take after dish powder and the mixing of 30% ethanolic solution at 50 DEG C lixiviate 5 hours;
2) sprouting of broccoli seeds: broccoli seeds is placed in 25 DEG C, carries out under the environment of 80% humidity cultivating, rudiment is until broccoli seeds shows money or valuables one carries unintentionally;
3) be rich in the cultivation of sulforaphen broccoli bud: be 20 DEG C by the broccoli seeds showed money or valuables one carries unintentionally in temperature, humidity is cultivate until broccoli bud grows to 8 ~ 10mm or broccoli root 10 ~ 12mm can gather under the environment of 80%.
In the broccoli bud that this implementation method is cultivated, sulforaphen content is 5.86mg/g.
Embodiment 3
Basic with above-described embodiment 1, its difference is:
1) selection of broccoli seeds and process: by select broccoli seeds be placed in mass concentration be 9% the expense dish crude flavonoid powder aqueous solution soak 8 hours; The fresh dish that takes to be pulverized with medicinal cracker after 24 hours 80 DEG C of dryings and crossed 80 mesh sieves, then will to take after dish powder and the mixing of 30% ethanolic solution at 60 DEG C lixiviate 4 hours;
2) sprouting of broccoli seeds: broccoli seeds is placed in 30 DEG C, carries out under the environment of 90% humidity cultivating, rudiment is until broccoli seeds shows money or valuables one carries unintentionally;
3) be rich in the cultivation of sulforaphen broccoli bud: be 25 DEG C by the broccoli seeds showed money or valuables one carries unintentionally in temperature, humidity is cultivate until broccoli bud grows to 8 ~ 10mm or broccoli root 10 ~ 12mm can gather under the environment of 90%.
In the broccoli bud that this implementation method is cultivated, sulforaphen content is 5.77mg/g.
Two, comparative trial
1, control group and processed group antiseptic effect compare (supplementing particular content)
Control group: seed germination under broccoli seeds → distilled water immersion 8 ~ 12 hours → distilled water flushing 3 times → 25 ~ 30 DEG C, 80 ~ 90% damp conditions → with 0.3 part of MS medium+99.7 parts of distilled water lucifuge, 25 ~ 30 DEG C, cultivate → treat broccoli bud bud under 80 ~ 90% damp conditions and grow to 8 ~ 10mm(root, 10 ~ 12mm) gather;
Processed group: broccoli seeds → 7 ~ 9% crude flavonoid powder solution soaks 8 ~ 12 hours → distilled water flushing 3 times → 25 ~ 30 DEG C, seed germination under 80 ~ 90% damp conditions → with prepare culture fluid certain illumination condition, 25 ~ 30 DEG C, cultivate → treat broccoli bud bud under 80 ~ 90% damp conditions and grow to 8 ~ 10mm(root, 10 ~ 12mm) gather.
Gather from broccoli seeds rudiment to broccoli bud, carry out record statistics to broccoli bud Decayed rate, statistics is as following table:
As seen from the above table: the Decayed rate of control group broccoli bud constantly raises and up to 57% when gathering in process of growth, and the Decayed rate of processed group broccoli bud suppresses in lower scope always.
2, control group and processed group results contrast
Gather from broccoli seeds rudiment to broccoli bud, record statistics is carried out to sulforaphen content in broccoli bud, statistics is illustrated in fig. 2 shown below, as shown in Figure 2: along with the growth of broccoli bud, sulforaphen content wherein constantly raises, when bud length for sulforaphen content during 8mm is the highest, when bud is grown up in 8mm, sulforaphen content decreases; In processed group broccoli bud, sulforaphen content is apparently higher than control group, and when bud long for processed group broccoli bud during 8mm in sulforaphen content than control group up to 60.06%.
Above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned citing.Those skilled in the art are in essential scope of the present invention, and the change made, remodeling, interpolation or replacement, also should belong to protection scope of the present invention.
Claims (3)
1. be rich in a breeding method for sulforaphen broccoli bud, it is characterized in that comprising the following steps:
(1) selection of broccoli seeds and process
Select when produce per year receive, without go mouldy and breakage, free from admixture, full grains broccoli seeds, the broccoli seeds of select is placed in mass concentration be 7 ~ 9% the expense dish crude flavonoid powder aqueous solution immersion 8 ~ 12 hours after, rinse 3 times with clear water; Described expense dish crude flavonoid powder preparation method is as follows: pulverized with medicinal cracker after 24 ~ 36 hours in 70 ~ 80 DEG C of dryings and cross 80 mesh sieves by fresh expense dish and obtain expense dish powder, to take after dish powder mixes in the ratio of solid-liquid ratio 1:50 with 30% ethanolic solution, lixiviate 4 ~ 5 hours at 50 ~ 60 DEG C, again under 3000r/min rotating speed after centrifugal 10min, obtain expense dish crude flavonoid powder leaching liquor after getting supernatant vacuum filtration, the freeze drying of expense dish crude flavonoid powder leaching liquor is obtained expense dish crude flavonoid powder;
(2) sprouting of broccoli seeds
Broccoli seeds after cleaning is placed in glass container or plastic containers, after adopting the multilayer gauze soaked through clear water to be covered by broccoli seeds, broccoli seeds is placed in temperature is 25 ~ 30 DEG C, humidity is carry out cultivation rudiment until broccoli seeds shows money or valuables one carries unintentionally under the environment of 80 ~ 90%;
(3) cultivation of sulforaphen broccoli bud is rich in
The broccoli seeds showed money or valuables one carries unintentionally is placed on the gauze soaked with self-control culture fluid, at certain illumination condition, temperature is 20 ~ 25 DEG C, humidity is cultivate under the environment of 80 ~ 90%, and sprayed self-control culture fluid every 24 hours, until broccoli bud grows to 8 ~ 10mm or the long 10 ~ 12mm of broccoli root can gather, obtain being rich in sulforaphen broccoli bud.
2. a kind of breeding method being rich in sulforaphen broccoli bud according to claim 1, it is characterized in that described self-control culture fluid raw material and parts by weight as follows:
3. a kind of breeding method being rich in sulforaphen broccoli bud according to claim 1, is characterized in that step (3) illumination condition is as follows for arranging:
00:00≤dark < 06:00
06:00≤white light < 12:00
12:00≤UV-C < 12:05 or 12:00≤UV-A < 12:05
12:05≤white light < 18:00
18:00≤dark < 24:00.
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| CN105851942A (en) * | 2016-03-30 | 2016-08-17 | 扬州大学 | Flavoring type high-isothiocyanate-content brassica juncea sprouts and production technology thereof |
| CN107019226A (en) * | 2017-03-10 | 2017-08-08 | 天津和治友德制药有限公司 | A kind of polypeptide powder and its preparation and application |
| CN110249833B (en) * | 2019-07-12 | 2021-06-29 | 中国农业科学院农业环境与可持续发展研究所 | Method for improving yield and quality of leaf vegetables in plant factory by low-dose long-wave ultraviolet light |
| CN111034730B (en) * | 2019-11-11 | 2021-06-15 | 宁波大学 | Method for cultivating bean sprouts rich in GABA and anthocyanin and method for preparing fermented milk beverage by using bean sprouts |
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| JPH10155378A (en) * | 1996-12-04 | 1998-06-16 | Sekisui Plastics Co Ltd | Water culture method and water culture device |
| DE102012011546A1 (en) * | 2012-06-09 | 2013-12-12 | Andreas Stihl Ag & Co. Kg | "Hand-guided implement" |
| CN103168606B (en) * | 2013-04-16 | 2015-06-17 | 镇江瑞繁农艺有限公司 | Method for cultivating broccoli in greenhouse at high yield and high efficiency |
| CN103380699B (en) * | 2013-08-06 | 2015-05-06 | 镇江瑞繁农艺有限公司 | Method for cutting and seedling raising of cauliflower male sterility line cluster buds |
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