CN102626029A - Method for planting ardisia crenata container seedlings by using microorganisms - Google Patents

Method for planting ardisia crenata container seedlings by using microorganisms Download PDF

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Publication number
CN102626029A
CN102626029A CN201210115446XA CN201210115446A CN102626029A CN 102626029 A CN102626029 A CN 102626029A CN 201210115446X A CN201210115446X A CN 201210115446XA CN 201210115446 A CN201210115446 A CN 201210115446A CN 102626029 A CN102626029 A CN 102626029A
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cave
dish
bicolor
matrix
microbial inoculum
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陈波
张楠
叶晓刚
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HANGZHOU LVFENG ECOLOGICAL GARDENS SCIENCE AND TECHNOLOGY DEVELOPMENT Co Ltd
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HANGZHOU LVFENG ECOLOGICAL GARDENS SCIENCE AND TECHNOLOGY DEVELOPMENT Co Ltd
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Priority to CN201210115446XA priority Critical patent/CN102626029A/en
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    • Y02P60/216

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Abstract

The invention provides a method for planting ardisia crenata container seedlings by using microorganisms. With adoption of glomus mosseae fungicide in cultivation, the seedlings have large amount of roots and grow better, and the cultivated seedlings after potting are easier to survive; the obtained cultivated plants grow strong and have compact plant type and high ornamental value; the cultivated seedlings have increased fruit-setting rate, bigger fruits and increased ornamental value; the pollution to soil and matrix is reduced and the seedlings are ecological and environmental-friendly; and the method is beneficial to production operation, is low in cost and can be used for cultivating ardisia plants.

Description

A kind of method of using microorganism to carry out the cultivation of bicolor container seedling
Technical field
The invention belongs to cultivation field, specifically is a kind of method of using microorganism to carry out the cultivation of bicolor container seedling.
Background technology
Cottage propagation is the important planting type of bicolor; For guaranteeing that its survival rate adopts chemical method, but be prone to cause soil, matrix to pollute more, Ardisa plant later stage sight is also had considerable influence; Loose like plant type, branch is elongated, fruit diminishes etc., is unfavorable for the sale of Ardisa plant.
?
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of method of using microorganism to carry out the cultivation of bicolor container seedling.
A kind of method of using microorganism to carry out the cultivation of bicolor container seedling is characterized in that comprising the steps:
1) gather bicolor and insert fringe, it is for use to sterilize, and prepares Glomus mosseae microbial inoculum, cave dish and cultivation matrix, and said Glomus mosseae microbial inoculum comprises spore, mycelia, mycorhiza sections and culture matrix;
2) 2/3 of required cultivation matrix is packed in the cave dish, the Glomus mosseae microbial inoculum evenly is layered on the cultivation matrix, will remain cultivation matrix again and pack into during the cave coils;
3) bicolor is inserted fringe and be inserted in the dish of cave, be positioned on the hot-house culture frame and cultivate, build shading screen, regular spray nutritious liquor, said nutrient solution comprises following component: the Ca (NO of 90-100 mg/L 3) 24H 2The KNO of O, 45-55 mg/L 3, 10-13 mg/L NH 4H 2PO 4, 25-30 mg/L K H 2PO 4MgSO with 45-55 mg/L 47H 2O;
When 4) growing to 2-3 month, change the cave dish, when growing to 3-5 month, change the cave dish once more, change the used cultivation matrix in dish back, cave and be the mixture that perlite, vermiculite, the peat composed of rotten mosses mix with the volume ratio of 2.5-3.5:2.5-3.5:1.5-2.5.
Described method; It is characterized in that gathering in the step 1) 2 years living bicolors and insert fringe; Bicolor inserts on the fringe and has at least 3 leaf segments, with for use behind 500 times of liquid surface sterilizations of 50% carbendazim 2min, gets the root system of plant of having planted one of plants such as red clover, New Zealand butch clover, clover, tobacco, Sudan grass and root system matrix on every side; Above-mentioned root system of plant shredded be 0.5cm left and right sides segment, admix 50% carbendazol wettable powder by 1 ~ 2g/kg and carry out surface sterilization.
Described method is characterized in that used cave dish is 72 holes dishes in the step 1), and said cave dish is cleaned up; With drying behind the 0.1% liquor potassic permanganate immersion 30min; Used cultivation matrix is a river sand, and said river sand at 120 ℃/0.1 MPa, 2 h that sterilize, and is regulated pH value to 5.5 ~ 6.5.
Described method is characterized in that in the step 1) bicolor that can bear fruit being bloomed preceding 1 month at it, with 800 times of liquid sprayings of 50% carbendazol wettable powder its plant and cultivation matrix is carried out disinfection.
Described method; It is characterized in that step 2) in comprise 60 Glomus mosseae microbial inoculum spores at least in every cave; To the bicolor that can bear fruit, evenly make a call to 5 ~ 6 Kong Zhiqi roots around bicolor plant one circle, the Glomus mosseae microbial inoculum is inserted in the cave; At least comprise 60 spores in every cave, with digging out next matrix backfill.
Described method, the temperature that it is characterized in that step 3) medium temperature chamber culturing rack is 25 ± 2 ℃, and the consumption of nutrient solution is the 30ml/ cave, whenever sprays once at a distance from 10 days, and the sunshade rate of shading screen is 65% ~ 75%, and temperature is 21 ~ 30 ℃ in the greenhouse, relative moisture is more than 80%.
Described method when it is characterized in that growing in the step 4) 2-3 month, is replaced by 50 holes or 32 holes dish, is replaced by the container of 5cm * 5cm or 6 cm * 6cm when growing to 3-5 month.
Described method is characterized in that nutrient solution comprises following component in the step 3): the Ca (NO of 93-97 mg/L 3) 24H 2The KNO of O, 47-52 mg/L 3, 11-12 mg/L NH 4H 2PO 4, 26-28 mg/L K H 2PO 4MgSO with 47-52 mg/L 47H 2O is preferably the Ca (NO of 94.5mg/L 3) 24H 2The KNO of O, 50.8mg/L 3, 11.5 mg/L NH 4H 2PO 4, 27.2 mg/L K H 2PO 4MgSO with 49.3 mg/L 47H 2O.
Described method is characterized in that nutrient solution also comprises following component in the step 3): the H of 0.25-0.3 mg/L 3BO 3, 0.15-0.2 mg/L MnCl 24H 2The ZnSO of O, 0.02-0.025 mg/L 47H 2The CuSO of O, 0.006-0.01 mg/L 45H 2The H of O, 0.008-0.012 mg/L 2MoO 44H 2The Na of O, 3.5-4 mg/L 2EDTA2H 2The FeSO of O and 2.5-3 mg/L 47H 2O is preferably the H of 0.268 mg/L 3BO 3, 0.181 mg/L MnCl 24H 2The ZnSO of O, 0.022 mg/L 47H 2The CuSO of O, 0.008 mg/L 45H 2The H of O, 0.009 mg/L 2MoO 44H 2The Na of O, 3.725mg/L 2EDTA2H 2The FeSO of O and 2.78mg/L 47H 2O.
Described method is characterized in that in the step 4) changing the used cultivation matrix in dish back, cave and is the mixture that perlite, vermiculite, the peat composed of rotten mosses mix with the volume ratio of 3:3:2.
A kind of method of using microorganism to carry out bicolor container seedling cultivation of the present invention has following advantage: root system is many, grow one-tenth work more easily behind the basin on the finished product; The finished product plant strain growth that obtains is healthy and strong, and plant type is compact, and ornamental value is high; Finished product seedling fruit-setting rate increases, and it is big that fruit becomes, and ornamental value increases; Reduced pollution, ecological, environmental protective to soil, matrix; Be beneficial to production operation; Cost is low, can be used for cultivating the Ardisa plant.
Embodiment
Embodiment 1
1. material is prepared
Vegetable material: the bicolor of gathering life in 2 years, stalwartness is inserted fringe, inserts on the fringe with the leaf segment more than 3, with for use behind 500 times of liquid surface sterilizations of 50% carbendazim 2min;
The AM fungal inoculant: Glomus mosseae Glomus mosseae microbial inoculum, form by spore, mycelia, mycorhiza sections and culture matrix.Red clover, New Zealand butch clover, clover, tobacco, Sudan grass etc. all are the good hosts who cultivates Glomus mosseae; Get and planted matrix around above-mentioned root system of plant and the root system; Above-mentioned root system of plant shredded be 0.5cm left and right sides segment, admix 50% carbendazol wettable powder by 1 ~ 2g/kg and carry out surface sterilization and can obtain a large amount of Glomus mosseae microbial inoculums.
Container and cultivation matrix: cuttage seeding is coiled with 72 holes, cleans up, and dries after soaking 30min with 0.1% liquor potassic permanganate, and matrix adopts river sand, and is at 120 ℃/0.1 MPa, 2 h that sterilize, subsequent use; Regulate cutting medium pH value to 5.5 ~ 6.5.
Bicolor to having beared fruit was bloomed preceding 1 month at it, with 800 times of liquid sprayings of 50% carbendazol wettable powder its plant and cultivation matrix was carried out disinfection.
2. inoculation microbial inoculum:
When being seeded to cutting medium; 2/3 of required river sand is packed in the cave dish, microbial inoculum evenly is layered on the river sand, comprise 60 spores in every cave at least; To remain river sand again and pack in the cave dish, all need pass through strictness with microbial inoculum and the contacted material of river sand and sterilize or disinfect.
Be seeded to the bicolor matrix that to have beared fruit, evenly make a call to 5 ~ 6 Kong Zhiqi roots, microbial inoculum is inserted in the cave, comprise 60 spores in every cave at least, digging out next matrix backfill around bicolor plant one circle.All and microbial inoculum and the contacted material of river sand all need pass through strict sterilization or disinfect.
3. cuttage and management:
Cuttage: preprepared is inserted during the fringe cuttage coils to the cave 1 the slotting fringe in every cave.Put to 25 ± 2 ℃ hot-house culture frame and cultivate, spray nutritious liquor 30ml/ cave, every later on separated 10d sprays once, and nutrient solution comprises the Ca (NO of 94.5mg/L 3) 24H 2The KNO of O, 50.8mg/L 3, 11.5 mg/L NH 4H 2PO 4, 27.2 mg/L K H 2PO 4MgSO with 49.3 mg/L 47H 2The H of O, 0.268 mg/L 3BO 3, 0.181 mg/L MnCl 24H 2The ZnSO of O, 0.022 mg/L 47H 2The CuSO of O, 0.008 mg/L 45H 2The H of O, 0.009 mg/L 2MoO 44H 2The Na of O, 3.725mg/L 2EDTA2H 2The FeSO of O and 2.78mg/L 47H 2O.Build shading screen after inserting, the sunshade rate is 65%-75%, and temperature is 21-30 ℃ in the greenhouse, and relative moisture is more than 80%, carries out Routine Management.When growing to 2-3 month, change basin, cuttage seeding is together moved in 50 holes, the 32 holes dish together with the river sand in the dish of cave, management method is identical during with cuttage.Growth of cuttage seedlings can change basin and to the container of 5cm * 5cm, 6 * 6cm, cultivate during to 3-5 month, can carry out suitable pinching, pruning according to the size of nursery stock etc. later on, and note changing basin.Changing the used matrix of basin is perlite: vermiculite: the peat composed of rotten mosses (volume ratio 3:3:2), the pH value transfers to 5.5-6.5, suitably adds into phosphate fertilizer, and notes current check, takes precautions against damage by disease and insect.
The result of embodiment 1 sees table 1 and table 2
The slotting fringe 30d growing state contrast of table 1 embodiment 1 inoculation Glomus mosseae microbial inoculum and other processing mode bicolors (P 0.05)
? The number of taking root Average root is long Rooting rate
Inoculation Glomus mosseae microbial inoculum 13.54a 9.97a 97.54a
CK (not processing) 4.83c 3.17c 56.73c
200 times of liquid of quick-acting root-inducing powders are handled 15s 7.21b 5.66b 79.23b
The contrast (P < 0.05) of bearing fruit of table 2 embodiment 1 inoculation Glomus mosseae microbial inoculum and other processing mode bicolors
? The number of blooming The fruit size The number of bearing fruit
Inoculation Glomus mosseae microbial inoculum 62.32a 0.75a 56.38a
CK (not processing) 61.22a 0.69b 40.12b
Embodiment 2
Embodiment 2 other steps are with embodiment 1, and nutrient solution comprises the Ca (NO of 93mg/L 3) 24H 2The KNO of O, 47mg/L 3, 11 mg/L NH 4H 2PO 4, 26 mg/L K H 2PO 4MgSO with 47 mg/L 47H 2The H of O, 0.25 mg/L 3BO 3, 0.15 mg/L MnCl 24H 2The ZnSO of O, 0.02 mg/L 47H 2The CuSO of O, 0.006 mg/L 45H 2The H of O, 0.008 mg/L 2MoO 44H 2The Na of O, 3.5mg/L 2EDTA2H 2The FeSO of O and 2.5mg/L 47H 2O.Changing the used matrix of basin is perlite: vermiculite: the peat composed of rotten mosses (volume ratio 2.5:2.5:1.5).
The result of embodiment 2 sees table 3 and table 4
The slotting fringe 30d growing state contrast of table 3 embodiment 2 inoculation Glomus mosseae microbial inoculums and other processing mode bicolors (P 0.05)
? The number of taking root Average root is long Rooting rate
Inoculation Glomus mosseae microbial inoculum 13.18a 9.65a 97.32a
CK (not processing) 4.83c 3.17c 56.73c
200 times of liquid of quick-acting root-inducing powders are handled 15s 7.21b 5.66b 79.23b
The contrast (P < 0.05) of bearing fruit of table 4 embodiment 2 inoculation Glomus mosseae microbial inoculums and other processing mode bicolors
? The number of blooming The fruit size The number of bearing fruit
Inoculation Glomus mosseae microbial inoculum 62.28a 0.74a 55.65a
CK (not processing) 61.22a 0.69b 40.12b
Embodiment 3
Embodiment 3 other steps are with embodiment 1, and nutrient solution comprises the Ca (NO of 97mg/L 3) 24H 2The KNO of O, 52mg/L 3, 12 mg/L NH 4H 2PO 4, 28 mg/L K H 2PO 4MgSO with 52 mg/L 47H 2The H of O, 0.3 mg/L 3BO 3, 0.2 mg/L MnCl 24H 2The ZnSO of O, 0.025 mg/L 47H 2The CuSO of O, 0.01 mg/L 45H 2The H of O, 0.012 mg/L 2MoO 44H 2The Na of O, 4mg/L 2EDTA2H 2The FeSO of O and 3mg/L 47H 2O.Changing the used matrix of basin is perlite: vermiculite: the peat composed of rotten mosses (volume ratio 3.5:3.5:2.5).
The result of embodiment 3 sees table 5 and table 6
The slotting fringe 30d growing state contrast of table 5 embodiment 3 inoculation Glomus mosseae microbial inoculums and other processing mode bicolors (P 0.05)
? The number of taking root Average root is long Rooting rate
Inoculation Glomus mosseae microbial inoculum 12.56a 9.81a 96.96a
CK (not processing) 4.83c 3.17c 56.73c
200 times of liquid of quick-acting root-inducing powders are handled 15s 7.21b 5.66b 79.23b
The contrast (P < 0.05) of bearing fruit of table 6 embodiment 3 inoculation Glomus mosseae microbial inoculums and other processing mode bicolors
? The number of blooming The fruit size The number of bearing fruit
Inoculation Glomus mosseae microbial inoculum 62.06a 0.72a 56.05a
CK (not processing) 61.22a 0.69b 40.12b
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all is included within protection scope of the present invention.

Claims (10)

1. a method of using microorganism to carry out the cultivation of bicolor container seedling is characterized in that comprising the steps:
1) gather bicolor and insert fringe, it is for use to sterilize, and prepares Glomus mosseae microbial inoculum, cave dish and cultivation matrix, and said Glomus mosseae microbial inoculum comprises spore, mycelia, mycorhiza sections and culture matrix;
2) 2/3 of required cultivation matrix is packed in the cave dish, the Glomus mosseae microbial inoculum evenly is layered on the cultivation matrix, will remain cultivation matrix again and pack into during the cave coils;
3) bicolor is inserted fringe and be inserted in the dish of cave, be positioned on the hot-house culture frame and cultivate, build shading screen, regular spray nutritious liquor, said nutrient solution comprises following component: the Ca (NO of 90-100 mg/L 3) 24H 2The KNO of O, 45-55 mg/L 3, 10-13 mg/L NH 4H 2PO 4, 25-30 mg/L K H 2PO 4MgSO with 45-55 mg/L 47H 2O;
When 4) growing to 2-3 month, change the cave dish, when growing to 3-5 month, change the cave dish once more, change the used cultivation matrix in dish back, cave and be the mixture that perlite, vermiculite, the peat composed of rotten mosses mix with the volume ratio of 2.5-3.5:2.5-3.5:1.5-2.5.
2. the method for claim 1; It is characterized in that gathering in the step 1) 2 years living bicolors and insert fringe; Bicolor inserts on the fringe and has at least 3 leaf segments, with for use behind 500 times of liquid surface sterilizations of 50% carbendazim 2min, gets the root system of plant of having planted one of plants such as red clover, New Zealand butch clover, clover, tobacco, Sudan grass and root system matrix on every side; Above-mentioned root system of plant shredded be 0.5cm left and right sides segment, admix 50% carbendazol wettable powder by 1 ~ 2g/kg and carry out surface sterilization.
3. the method for claim 1; It is characterized in that used cave dish is 72 holes dishes in the step 1); Said cave dish is cleaned up, and with drying behind the 0.1% liquor potassic permanganate immersion 30min, used cultivation matrix is a river sand; Said river sand at 120 ℃/0.1 MPa, 2 h that sterilize, and is regulated pH value to 5.5 ~ 6.5.
4. the method for claim 1 is characterized in that in the step 1) bicolor that can bear fruit being bloomed preceding 1 month at it, with 800 times of liquid sprayings of 50% carbendazol wettable powder its plant and cultivation matrix is carried out disinfection.
5. the method for claim 1; It is characterized in that step 2) in comprise 60 Glomus mosseae microbial inoculum spores at least in every cave; To the bicolor that can bear fruit, evenly make a call to 5 ~ 6 Kong Zhiqi roots around bicolor plant one circle, the Glomus mosseae microbial inoculum is inserted in the cave; At least comprise 60 spores in every cave, with digging out next matrix backfill.
6. the method for claim 1, the temperature that it is characterized in that step 3) medium temperature chamber culturing rack is 25 ± 2 ℃, the consumption of nutrient solution is the 30ml/ cave; Whenever sprayed once at a distance from 10 days; The sunshade rate of shading screen is 65% ~ 75%, and temperature is 21 ~ 30 ℃ in the greenhouse, and relative moisture is more than 80%.
7. the method for claim 1 when it is characterized in that growing in the step 4) 2-3 month, is replaced by 50 holes or 32 holes dish, is replaced by the container of 5cm * 5cm or 6 cm * 6cm when growing to 3-5 month.
8. the method for claim 1 is characterized in that nutrient solution comprises following component in the step 3): the Ca (NO of 93-97 mg/L 3) 24H 2The KNO of O, 47-52 mg/L 3, 11-12 mg/L NH 4H 2PO 4, 26-28 mg/L K H 2PO 4MgSO with 47-52 mg/L 47H 2O is preferably the Ca (NO of 94.5mg/L 3) 24H 2The KNO of O, 50.8mg/L 3, 11.5 mg/L NH 4H 2PO 4, 27.2 mg/L K H 2PO 4MgSO with 49.3 mg/L 47H 2O.
9. the method for claim 1 is characterized in that nutrient solution also comprises following component in the step 3): the H of 0.25-0.3 mg/L 3BO 3, 0.15-0.2 mg/L MnCl 24H 2The ZnSO of O, 0.02-0.025 mg/L 47H 2The CuSO of O, 0.006-0.01 mg/L 45H 2The H of O, 0.008-0.012 mg/L 2MoO 44H 2The Na of O, 3.5-4 mg/L 2EDTA2H 2The FeSO of O and 2.5-3 mg/L 47H 2O is preferably the H of 0.268 mg/L 3BO 3, 0.181 mg/L MnCl 24H 2The ZnSO of O, 0.022 mg/L 47H 2The CuSO of O, 0.008 mg/L 45H 2The H of O, 0.009 mg/L 2MoO 44H 2The Na of O, 3.725mg/L 2EDTA2H 2The FeSO of O and 2.78mg/L 47H 2O.
10. the method for claim 1 is characterized in that changing in the step 4) the used cultivation matrix in dish back, cave and is the mixture that perlite, vermiculite, the peat composed of rotten mosses mix with the volume ratio of 3:3:2.
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CN105145266A (en) * 2015-09-29 2015-12-16 中国科学院华南植物园 Ardisia dasyrhizomatica propagation method
CN105722571A (en) * 2014-05-19 2016-06-29 横滨橡胶株式会社 Rubber composition, rubber composition metal laminate, and vulcanized rubber product
CN106818106A (en) * 2016-12-12 2017-06-13 长江大学 A kind of method for promoting hyacinth Blooming and kind of blooming period prolonging
CN108990571A (en) * 2018-07-12 2018-12-14 杭州富阳飞博科技有限公司 A kind of matrix for maple cuttage
CN108990669A (en) * 2018-08-14 2018-12-14 孙宝泉 The oil method for culturing seedlings of tree peony
CN109041841A (en) * 2018-08-01 2018-12-21 杭州富阳飞博科技有限公司 The rapid cuttage technique of red maple
CN109168961A (en) * 2018-10-31 2019-01-11 芒市喜丰农业发展有限公司 A kind of cultural method of white ginseng
CN113016620A (en) * 2021-04-19 2021-06-25 贵州中医药大学 Method for rapid propagation of ardisia crenata tissue

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Publication number Priority date Publication date Assignee Title
CN103875503A (en) * 2014-01-16 2014-06-25 中国科学院新疆生态与地理研究所 Method for increasing survival rate in transplanting populus euphratica shoots in extremely arid desert area
CN105722571A (en) * 2014-05-19 2016-06-29 横滨橡胶株式会社 Rubber composition, rubber composition metal laminate, and vulcanized rubber product
CN105722571B (en) * 2014-05-19 2017-03-01 横滨橡胶株式会社 Rubber composition, rubber composition metal laminate and vulcanized rubber article
CN105145266A (en) * 2015-09-29 2015-12-16 中国科学院华南植物园 Ardisia dasyrhizomatica propagation method
CN106818106A (en) * 2016-12-12 2017-06-13 长江大学 A kind of method for promoting hyacinth Blooming and kind of blooming period prolonging
CN106818106B (en) * 2016-12-12 2020-07-31 长江大学 Method for promoting hyacinth to bloom in advance and prolonging flowering phase
CN108990571A (en) * 2018-07-12 2018-12-14 杭州富阳飞博科技有限公司 A kind of matrix for maple cuttage
CN109041841A (en) * 2018-08-01 2018-12-21 杭州富阳飞博科技有限公司 The rapid cuttage technique of red maple
CN108990669A (en) * 2018-08-14 2018-12-14 孙宝泉 The oil method for culturing seedlings of tree peony
CN109168961A (en) * 2018-10-31 2019-01-11 芒市喜丰农业发展有限公司 A kind of cultural method of white ginseng
CN113016620A (en) * 2021-04-19 2021-06-25 贵州中医药大学 Method for rapid propagation of ardisia crenata tissue
CN113016620B (en) * 2021-04-19 2022-10-18 贵州中医药大学 Method for rapid propagation of ardisia crenata tissue

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