CN106472289A - Herba Dendrobii lichen association culture systems altogether - Google Patents
Herba Dendrobii lichen association culture systems altogether Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The present invention relates to a kind of Herba Dendrobii lichen association culture systems altogether, belong to gardening technical field of forestry.Combining of association lichen or association lichen and bark is coated on the base of resistance to rotten wood, the base of resistance to rotten wood, association lichen/association lichen and bark form supporter, by fixing for candidum tissue culturing seedling on a support, be fixed on tree shade masking forest land, you can.Invention is applied to the plantation of Herba Dendrobii, has the advantages that achievable stereo planting, raising forest zone are affected using area, minimizing growing environment.
Description
Technical field
The present invention relates to a kind of Herba Dendrobii-lichen association culture systems altogether, belong to gardening technical field of forestry.
Background technology
Herba Dendrobii（Dendrobium officinale Kimura et Migo）It is China's tradition rare Chinese medicine, main
The ground such as Anhui to be distributed in, Zhejiang, Fujian.Have functions that reinforcing stomach reg fluid, nourishing YIN and clearing away heat etc. are unique.Early in Qin Han dynasty,《God
Agriculture book on Chinese herbal medicine warp》" sweet in the mouth is put down just to record Herba Dendrobii.Main damaging the spleen and stomach, eliminating impediment, the therapeutic method to keep the adverse QI flowing downwards, tonifying five ZANG-organses asthenia, frail, reinforcing YIN-essence.Long term usage is thick
The intestines and stomach, make light of one's life by commiting suicide, macrobiosis ".Before more than 1000 year《Taoist Scriptures》Herba Dendrobii is classified as first of " Chinese nine big Herba mesonae chinensis "；Li Shizhen (1518-1593 A.D.) exists
《Compendium of Materia Medica》" reinforcing YIN-essence benefit essence, thick the intestines and stomach, never sufficient in benefit, flat stomach gas, long muscle, Fructus Alpiniae Oxyphyllae removes frightened, gently middle evaluation Herba Dendrobii
Body macrobiosis ".Among the people be called help Herba mesonae chinensis, international medicinal plants circle be referred to as " medicine circle giant panda ".Modern pharmacological research proves, ferrum
Skin Herba Dendrobii has enhancing immunity, eliminates the effects such as tumor, suppression cancer, to laryngopharyngeal diseasess, gastroenteropathy, cataract, painstaking effort
Pipe disease, diabetes, tumor are respectively provided with significant curative effect.For this reason, version in 2010《Chinese Pharmacopoeia》It is individually recorded.20th century
Before the nineties, Herba Dendrobii relies primarily on wild resource.Due to crushing excavation, the destruction of living environment and self-reproduction
Inferior capabilities, wild resource is substantially exhausted, and State Council is classified as Chinese Second Class Key Protected Plant within 1987.2011, national people
Work cultivated area breaks through more than 10000 mu, and the medicine with Herba Dendrobii as raw material is individual with health product more than 30, defines from Herba Dendrobii
The complete industrial chain of plantation, processing, sale, the Herba Dendrobii output value, more than 3,000,000,000 yuan, becomes the maximum health care of domestic production-sales volume and produces
One of product.
At present, the cultivation of Herba Dendrobii and scale plantation are broadly divided into facility emulational growth and Lin Nei ecologic planting mould
Formula two big class, respectively there are different pluses and minuses in two kinds of planting patternss.Facility emulational growth, that is, utilize glasshouse or plastics
The facilities such as thin film booth, with bark etc. as cultivation matrix, are equipped with the facilities such as sunshade net, spraying and irrigation, imitate wild environment training
Educate a kind of cultural method of Herba Dendrobii, its advantage is that the conditions such as temperature, humidity, fertility control precisely, Growth of Dendrobium candidum speed
Degree is very fast, but due to not having the effect of natural environmental factors, the accumulation of secondary substance is inadequate；Ecologic planting pattern in woods, mainly
There are cultivation under woods and the tree that lives to grow nonparasitically upon another plant two kinds of original ecology cultivating pattern, mainly make full use of the natural environmental condition effect in woods,
Reach the purpose of original ecology cultivating, its advantage is that growing environment manual intervention is less, belong to and completely grow under natural environment, adopt
The quality of Herba Dendrobii afterwards is high, and secondary substance accumulation is many.But growth is relatively slow, and the forest land utilization rate that condition is suitable for is low,
Yield is rare, simultaneously as forest land arboreal growth is irregular, the landform such as mountain forest slope, and also result in that growing environment is unstable, water
Stream washes away invasion and attack, impact plantation survival rate and growth stability to forest land plantation.
Based on this, make the application.
Content of the invention
For the drawbacks described above in the presence of existing Herba Dendrobii planting process, the application provides a kind of achievable three-dimensional kind
Plant, improve the Herba Dendrobii stereo plantation method that forest zone utilizes area, minimizing growing environment to affect.
Herba Dendrobii-lichen association culture systems altogether, are coated association lichen or association lichen and bark on the base of resistance to rotten wood
Combination, the base of resistance to rotten wood, association lichen/association lichen and bark form supporter, candidum tissue culturing seedling are fixed on
On supporter, it is fixed in tree shade masking forest land（Refer to vertically, the scope below branch is tree shade shaded areas）
On, you can.
Further, as preferred：
Culture medium is fixed with the described base of resistance to rotten wood, lichen is attached to proliferation in this culture medium, and is coated on resistance to rotten wood
On base.It is furthermore preferred that described lichen need to carry out disinfection being adhered to again；Described culture medium be Knop fluid medium,
Benecke fluid medium, starch, glucose, washing water of rice, vegetable washing water or river, above-mentioned culture medium soaks plank, takes lichen,
Add liquid nitrogen, clayed into power, be uniformly coated on plank, 25 DEG C, the low light level, spraying and moisturizing is cultivated, you can make lichen attached
In culture medium；Described lichen carries out maintenance in spraying and moisturizing mode.
Before described candidum tissue culturing seedling is fixing, supporter is sprayed with symbiosis bacterium solution.
Described association lichen is coated on the base of resistance to rotten wood in single or multiple lift mode.
Described candidum tissue culturing seedling is fixing on a support in single or multiple lift mode, when being set to multilamellar, ferrum
Skin Herba Dendrobii can divide multilamellar to arrange on monolayer association lichen, also respectively can arrange one layer on every layer of association lichen.
Spacing between described supporter is adjacent is 0.8-1.5 times of supporter height.
The operation principle of the application and having the beneficial effect that：
（1）Improve forest zone utilization rate, reduce forest zone physical features impact.The application is using the base of resistance to rotten wood as the support of stereoscopic culture
Thing, by candidum tissue culturing seedling, single or multiple lift is fixed on this supporter on demand, it is sequentially fixed on tree shade masking
On forest land, so that the culture of Herba Dendrobii is sprawled to vertically spatially extending from monolayer, not only increase forest zone utilization rate with identical
Implantation Rate on area, also by the growing environment of Herba Dendrobii by ground moving to earth's surface overhead, former farthest meeting
While ecological culture, also fundamentally avoid the impact that physical features causes to incubation, effectively solve environment suitable
Forest land arboreal growth is irregular, mountain forest slope current wash away, to forest land plantation, the harm that invasion and attack bring, and can effectively reduce mountain region
The pollution risk of the harmful substance such as heavy metal that may be present in soil, is that the plantation of Herba Dendrobii provides stable ring of growing nonparasitically upon another plant
Border, thus widened Herba Dendrobii plantation can favored area, be the cultivation systems that can effectively improve suitable forest land utilization rate.
（2）Using bryophyte as syntaxial system, tissue culture technology is combined with Mycorrhizal Technology, Herba Dendrobii can be accelerated
Organic growth.The important auxiliary microenvironment structure person in cultivating as wild plant of aulophyte, particularly bryophyte, to resistance to
Rotten wood primary surface carries out structure treatment, makes it fixing internal layer lichen and cultivates substrate, by proliferation mode, so that lichen is coated
Outside the base of resistance to rotten wood, when moss growth, what it can be spontaneous absorbs to moisture, thus being the iron sheet stone grown nonparasitically upon another plant on supporter
Dry measure used in former times provides moisture, be most important water-retaining property accompanying plant in dendrobe cultivation, it is to avoid drying, hydropenia, Shi Shui irregular to ferrum
The impact that the growth of skin Herba Dendrobii causes, and provide secondary substance for Herba Dendrobii in growth course, thus effectively accelerating ferrum
The organic growth of skin Herba Dendrobii.
（3）Fungal component ensure that whole supporter sets up the microenvironment of its its growth suitable for Herba Dendrobii, contributes to ferrum
Effectively the growing nonparasitically upon another plant of skin Herba Dendrobii.It is fixed on supporter in Herba Dendrobii to go forward, first on the base of resistance to rotten wood, spray symbiosis bacterium solution, and formed
Fungal component layer, when Herba Dendrobii is fixed on this supporter, its initial environment is very beneficial for the growth of Herba Dendrobii, its with
The endophyte producing in moss growth cooperates, and while promoting endophyte and moss growth, is also beneficial to Herba Dendrobii and exists
Organic growth on supporter.
The application, using bryophyte as association system, using the base of resistance to rotten wood as supporter of growing nonparasitically upon another plant, in conjunction with fungal component, builds
Rotproofness timber is coated the plan ecologic planting association system altogether of the solid containing Herba Dendrobii fungal component and association bryophyte,
The utilization of limits is suitable for the limited forest resourceies of Growth of Dendrobium candidum, and utilizes beneficial symbiotic effects and association bryophyte
The microecological environment being formed with Herba Dendrobii, suppresses the growth of other harmful bacterias, and provides growth required water and nutrient, realizes
Take into account high-quality-high yield, and reduce the green three-dimensional plan ecological cultivation pattern of disease.It is effectively protected this country two simultaneously
Level protection plant, is conducive to the recovery of wild stockss, for protecting species diversity, also for the sustainable profit of Chinese medicine natural resourcess
With providing approach.
Brief description
Fig. 1 is the side view of the application；
Fig. 2 is the top view of the application；
Fig. 3 is the structural representation of supporter in the application；
The impact that Fig. 4-1 cultivates to Moss tissue for Benecke culture medium；
The impact that Fig. 4-2 cultivates to Moss tissue for Knop culture medium；
Fig. 4-3 is the sprouting in Knop culture medium, Moss tissue culture being grown；
Fig. 5-1 is growing of endophyte；
Fig. 5-2 is the separation of lichen endophyte；
Fig. 6-1 is the PCR identification of lichen endophyte；
Fig. 6-2 is the DNA extraction of lichen endophyte；
Fig. 7-1 is the different culture media matter of lichen；
Fig. 7-2 is the situation of growing nonparasitically upon another plant of lichen under no substrate status；
Fig. 7-3 is the situation of growing nonparasitically upon another plant of lichen under covering state；
The situation of growing nonparasitically upon another plant that Fig. 7-4 is lichen under substrate status for river；
Fig. 7-5 is the situation of growing nonparasitically upon another plant of lichen during river substrate 20 days；
Fig. 7-6 is the situation of growing nonparasitically upon another plant of lichen during river substrate 30 days；
Fig. 8-1 is the upgrowth situation as candidum tissue culturing seedling during fixing culture for the rotten wood+lichen；
Fig. 8-2 is the upgrowth situation as candidum tissue culturing seedling during fixing culture for the rotten wood+lichen+Cortex Pini；
Fig. 8-3 is the upgrowth situation as candidum tissue culturing seedling during fixing culture for the Cortex Pini+rotten wood；
Fig. 8-4 is the upgrowth situation as candidum tissue culturing seedling during fixing culture for the rotten wood；
Fig. 9-1 is the root system development outside drawing of the Herba Dendrobii under different condition of culture；
Fig. 9-2 is the dry and wet weight block diagram of the Herba Dendrobii under different condition of culture；
Figure 10 is another structural representation of supporter in the application.
Wherein label：1. tree shade masking forest land；2. supporter；3. the base of resistance to rotten wood；4. fungal component layer；5. association tongue
Moss layer.
Specific embodiment
Materials and methods
1.1 experiment material
Lichen：Nearby on the tree of park, on wall, the lichen of shady place is multiple.
Herba Dendrobii：The tissue cultured seedling in Zhejiang Province large-scale iron sheet growing dendrobium base.
Key instrument：Fluorescence microscope（Nikon）、PCR（Bio-rad）, gel imaging system（Bio-rad）, nucleic acid
Protein analyzer（Bio-rad）.
Experimental technique
1.2.1 the sterilization of lichen
Choose fresh lichen, soak 30min with 0.1% sodium hypochlorite, and shake 4 hours on shaking table, distilled water immersion is cleaned, use
Aseptic filter paper suck dry moisture.
The configuration of lichen culture medium
Moss tissue culture medium selects Knop culture medium and two kinds of Benecke culture medium.
Lichen DNA extraction and identification
Take fresh lichen, add liquid nitrogen grinding, add CTAB（Containing 0.1% mercaptoethanol）, 65 DEG C insulation 90Min, chloroform or
Chloroform：Isoamyl alcohol, mixes, and is centrifuged 10000g 10min after being divided into.Aqueous phase is transferred in new pipe, adds the ice second of 2 times of volumes
Alcohol, with the TE dissolving containing RNase.
The separation of lichen endophyte and culture
The lichen adopted back is cleaned with tap water, and uses aseptic paper suck dry moisture, and uses aseptic paper suck dry moisture, cuts trickle little
Block, with 75% alcohol-pickled 5m in, 7m in, 10m in, processes 4 min, 6 min, 8 min with 0.1% mercuric chloride solution respectively,
Carry out surface sterilizing, this test is repeated 3 times respectively, finally use aseptic water washing 4 times.Piece of tissue is fully ground in mortar
Become homogenate, last flushing liquor applies 3 kinds of different culture media flat boards（PDA, Ma Dingshi, benecke culture medium）, 26 ~ 28 DEG C
Lower culture 2 ~ 3d, filters out optimal disinfecting time and culture medium, and sterilizing mortar is put in thorough for surface sterilization cotton root stripping and slicing
Middle use aseptic water washing 4 times, takes supernatant to be applied in culture medium, with aseptic water washing as controlled trial.Supernatant homogenate is taken to apply
Smear on 3 kinds of different culture medias, take homogenate to coat different culture media flat board, every concentration is repeated 3 times, and then puts culture dish
Cultivate 2 ~ 3d in 26 ~ 28 DEG C of calorstats, observe the bacterium colony shape turned out.
Lichen endophyte DNA extraction and identification
Select the bacterial strain of purification, shake bacterium 48h at 28 DEG C；After taking 1ml centrifugation, milled breaking cellular wall with liquid nitrogen, extracted using CTAB method
DNA, sepharose electrophoresis detect DNA extraction content.
Using ribosome rDNA area universal primer ITS1：TCCGTAGGTGAACCTGCGG and ITS4：
TCCTCCGCTTATTGATATGC, enters performing PCR amplification, 55 DEG C of fiery temperature；Tentatively judge primer size using sepharose electrophoresis.
Lichen adheres to
Knop fluid medium, Benecke fluid medium, starch, glucose, washing water of rice, vegetable washing water, river etc. is used to make respectively
Soak plank for culture medium.Take lichen, add liquid nitrogen, clayed into power, be uniformly coated on plank, 25 DEG C, the low light level, spray
Mist moisturizing is cultivated.
The maintenance of lichen
Spray four times a day, keep moistening, 25 DEG C, the low light level.
The fixation of Herba Dendrobii and maintenance
By Herba Dendrobii Caulis et Folium Oryzae be fixed on rotten wood, the rotten wood of sterilized Cortex Pini, on sterilized rotten wood, unpasteurized pine
On the rotten wood of bark, compare experiment.Wherein, in conjunction with Fig. 3, the key component of supporter 2 is the base of resistance to rotten wood 3, that is, implement
The rotten wood selected in example, also can be selected for the rotten wood with bark；It is provided with supporting layer and fungal component layer 4 is formed by symbiosis bacterium solution, should
Fungal component layer 4 can be located between association lichen layer 5 and the base of resistance to rotten wood 3, may also be arranged on association lichen layer 5 surface；And lichen exists
Association lichen layer 5 is then formed on the base of resistance to rotten wood 3；In conjunction with Fig. 1 and Fig. 2, it is fixed with the supporter 2 of candidum tissue culturing seedling sequentially
Be fixed on tree shade masking forest land 1, spacing between adjacent supports thing 2 be respectively supporter 2 height 0.8,0.9,1.0,
1.1st, 1.2,1.3,1.4,1.5 times.One group of fungal component layer 4+ association lichen layer 5 can be arranged as shown in Figure 3 on supporter 2,
Multigroup fungal component layer 4+ association lichen layer 5 can be set as shown in Figure 10.
Result and analysis
The impact that 2.1 different culture media conditions are cultivated to Moss tissue
Using Knop culture medium and two kinds of culture medium of Benecke culture medium, tissue culture is carried out to the lichen of sterilization.Benecke
Three batches of lichens of culture medium culturing, do not have sprouting to grow；Start to grow sprouting after two weeks in Knop culture medium, compare two kinds of cultures
Base, many ferrous ions of reduced form in Knop culture medium, referring specifically to Fig. 4-1,4-2,4-3.
The separation of lichen endophyte
The young moss seedlings subculture of two weeks will be cultivated on Knop culture medium, after being further cultured for two weeks in new culture medium, long around lichen
Go out white collarium and be endophyte.Choose bacterium colony to cultivate in fungi screening culture medium.Through two generations after purification, liquid culture, tool
Body is referring to Fig. 5-1,5-2.
The DNA extraction of lichen endophyte and PCR identification
（1）Select the bacterial strain of purification, shake bacterium 48h at 28 DEG C.
（2）After going 1ml centrifugation, milled breaking cellular wall with liquid nitrogen, extract DNA using CTAB method, sepharose electrophoresis detect DNA extraction
Content.
（3）Using ribosome rDNA area universal primer ITS1：TCCGTAGGTGAACCTGCGG and ITS4：
TCCTCCGCTTATTGATATGC, enters performing PCR amplification, 55 DEG C of fiery temperature.
（4）Tentatively judge primer size using sepharose electrophoresis, extra large handsome survey is served to the bacterial strain PCR primer having band
Sequence, sequencing result is compared in GENBANK, referring specifically to Fig. 6-1,6-2.
The impact that different substrates are grown nonparasitically upon another plant to lichen
Knop fluid medium, Benecke fluid medium, starch, glucose, washing water of rice, vegetable washing water, river etc. is used to make respectively
Soak plank for culture medium.Take lichen, add liquid nitrogen, clayed into power, be uniformly coated on plank, 25 DEG C, the low light level, spray
Mist moisturizing is cultivated.
Referring to Fig. 7-1,7-2,7-3,7-4,7-5 and 7-6, result shows：The preferable substrate of nutrition, and it is unfavorable for lichen
Growth, river soaks the plank of two weeks, and lichen growing way is best.
The fixing culture of different substrates
By Herba Dendrobii Caulis et Folium Oryzae be fixed on rotten wood, the rotten wood of sterilized Cortex Pini, on sterilized rotten wood, unpasteurized pine
On the rotten wood of bark, compare experiment.Referring to Fig. 8-1,8-2,8-3,8-4, result shows, Herba Dendrobii of growing nonparasitically upon another plant on rotten wood
Growing way is best.
Growth of Dendrobium candidum situation under different condition of culture compares
Herba Dendrobii under different condition of culture is taken out, observes its root system development situation, referring specifically to Fig. 9-1；Weigh it to do
Weight and weight in wet base, referring to Fig. 9-2, from Fig. 9-2 as can be seen that the lichen Herba Dendrobii root system that association is cultivated altogether is sturdy, weight in wet base dry weight
It is higher than all other training methods.
Spacing between adjacent supports thing 2 be respectively supporter 2 height 0.8,0.9,1.0,1.1,1.2,1.3,1.4,
When 1.5 times, all can achieve the matched well of total revenue and Herba Dendrobii growing way, but when 1.0-1.3 times, especially this spacing is
When 1.1 times of supporter 2 height, candidum tissue culturing seedling does not have the masking of significantly mutually low-light each other, can obtain
To relatively more excellent growth data.
The effective ingredient of Herba Dendrobii is exactly dendrobium polysaccharide content, and the key that this is also embodiment planting effect refers to
Mark, general wild artificial culture is 10% for 20% about, and the Herba Dendrobii of the application plantation, its liquid chromatographic detection
The biennial polyoses content of dendrobium polysaccharide content close to 30%, annual 25%, be above conventional manual's cultivation result, quality
Can compare favourably with dendrobium officinale, but the speed of growth is far above dendrobium officinale.
The application is used as association system by bryophyte, using timber as supporter of growing nonparasitically upon another plant, in conjunction with fungal component, builds vertical
Body intends ecologic planting pattern, and the planting conditions of the lichen of association are explored, and gropes association condition, inquires into multiple plantations
Under the conditions of, the upgrowth situation of Herba Dendrobii.Constructed three-dimensional ecologic planting pattern of intending has following two big advantages：（1）Due to
This project screening and the common association system system our province indigenous flora cultivated and lichen, its climate adaptability is stronger, is conducive at this
Later stage application is carried out on ground and wild rare resources are recovered；（2）This system can utilize to greatest extent and be suitable for Growth of Dendrobium candidum
Limited forest resourceies, and the microecological environment being formed with Herba Dendrobii using beneficial symbiotic effects and association bryophyte, suppression
Make the growth of other harmful bacterias, and provide growth required water and nutrient, realize taking into account high-quality-high yield, and reduce disease
Green three-dimensional intend ecological cultivation pattern.
Above content be preferred implementation with reference to the invention provided technical scheme is made detailed further
Describe in detail bright it is impossible to assert that the invention is embodied as being confined to these explanations above-mentioned, the affiliated technology for the invention
For the those of ordinary skill in field, without departing from the concept of the premise of the invention, some simple deductions can also be made
Or replace, all should be considered as belonging to the protection domain of the invention.
Claims (9)
1. Herba Dendrobii-lichen altogether association culture systems it is characterised in that：Lichen or lichen and bark are coated on the base of resistance to rotten wood
Combination, the base of resistance to rotten wood, lichen/lichen and bark form supporter, by fixing for candidum tissue culturing seedling on a support,
It is fixed on tree shade masking forest land, you can.
2. Herba Dendrobii-lichen as claimed in claim 1 altogether association culture systems it is characterised in that：Described lichen is with list
Layer or multilamellar mode are coated on the base of resistance to rotten wood.
3. Herba Dendrobii-lichen as claimed in claim 1 altogether association culture systems it is characterised in that：Described Herba Dendrobii
Before tissue cultured seedling is fixing, supporter is sprayed with symbiosis bacterium solution.
4. Herba Dendrobii-lichen as claimed in claim 1 altogether association culture systems it is characterised in that：Described Herba Dendrobii
Tissue cultured seedling is fixing on a support in single or multiple lift mode.
5. Herba Dendrobii-lichen as claimed in claim 1 altogether association culture systems it is characterised in that：Described supporter phase
Spacing between neighbour is 0.8-1.5 times of supporter height.
6. Herba Dendrobii-the lichen as described in claim 1-5 altogether association culture systems it is characterised in that：Described resistance to rotten wood
Culture medium is fixed with base, lichen is attached in this culture medium and carries out proliferation, and is coated on the base of resistance to rotten wood.
7. Herba Dendrobii-lichen as claimed in claim 6 altogether association culture systems it is characterised in that：Described lichen need to enter
Row sterilization is adhered to again.
8. Herba Dendrobii-lichen as claimed in claim 6 altogether association culture systems it is characterised in that：Described culture medium is
Knop fluid medium, Benecke fluid medium, starch, glucose, washing water of rice, vegetable washing water or river.
9. Herba Dendrobii-lichen as claimed in claim 6 altogether association culture systems it is characterised in that：Described lichen is to spray
Mist moisturizing mode carries out maintenance.
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| CN109463254A (en) * | 2018-12-07 | 2019-03-15 | 昆明理工大学 | A method of accelerate barren rock at soil using moss |
| CN109924114A (en) * | 2018-11-21 | 2019-06-25 | 赣南树木园 | One kind vacantly pasting compound dendrobium candidum underwood planting structure and implantation methods |
| CN112205239A (en) * | 2020-10-29 | 2021-01-12 | 福建省连城冠江铁皮石斛有限公司 | Microbial culture solution and method for cultivating fungi on cliff wall and symbiotic planting of dendrobium |
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| CN107182735B (en) * | 2017-04-27 | 2019-10-25 | 江西九草铁皮石斛科技协同创新有限公司 | A kind of method for culturing seedlings and cultural method of live streaming formula dendrobium candidum |
| CN109924114A (en) * | 2018-11-21 | 2019-06-25 | 赣南树木园 | One kind vacantly pasting compound dendrobium candidum underwood planting structure and implantation methods |
| CN109924114B (en) * | 2018-11-21 | 2023-05-26 | 赣南树木园 | Suspended compound dendrobium candidum under-forest planting structure and planting method |
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| CN112205239A (en) * | 2020-10-29 | 2021-01-12 | 福建省连城冠江铁皮石斛有限公司 | Microbial culture solution and method for cultivating fungi on cliff wall and symbiotic planting of dendrobium |
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