CN107182735A - A kind of method for culturing seedlings and cultural method of live formula dendrobium candidum - Google Patents
A kind of method for culturing seedlings and cultural method of live formula dendrobium candidum Download PDFInfo
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- CN107182735A CN107182735A CN201710289152.1A CN201710289152A CN107182735A CN 107182735 A CN107182735 A CN 107182735A CN 201710289152 A CN201710289152 A CN 201710289152A CN 107182735 A CN107182735 A CN 107182735A
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- dendrobium candidum
- moss
- liver moss
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- culturing seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention principally falls into dendrobium candidum nursery field, and in particular to a kind of method for culturing seedlings and cultural method of live formula dendrobium candidum.Seeds of Dendrobium Candidum is directly seeded on liver moss by methods described, and the Seeds of Dendrobium Candidum completes seed germination and process of emerging in the liver moss；The moss growth is on moss growth hypothallus.The method for culturing seedlings utilizes liver moss to carry out live formula nursery, it is to avoid the shortcoming of tissue culture；The seedling that the cultural method obtains studio's method for culturing seedlings carries out implanting and cultivating together with liver moss and moss growth hypothallus, it is possible to increase survival rate of the dendrobium candidum in cultivation.
Description
Technical field
The present invention principally falls into dendrobium candidum nursery field, and in particular to a kind of method for culturing seedlings of live formula dendrobium candidum and
Cultural method.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) alias iron sheet is blue, ribbed hedyotis herb, and category is blue
The perennial herbaceous plant that grows nonparasitically upon another plant of section Dendrobium, is one of most precious Chinese medicine of China, because with " reinforcing stomach reg fluid, nourishing Yin and clearing heat "
Effect and the extensive scope of application and be gradually known.With integrated, the plantation of dendrobium candidum of planting technology
Industry realizes scale and intensive development, and cultivated area and yield increase year by year.
The breeding of current dendrobium candidum mainly obtains seedling by way of Plant Tissue Breeding, and cultivation is mainly
Seedling is used as using tissue-cultured seedling.Plant Tissue Breeding is that plant Sterile Culture Methods Used is the reason for having totipotency according to plant cell
By utilizing the in vitro organ of plant such as root, stem, leaf, stem apex, flower, fruit etc.) tissue (such as forming layer, epidermis, cortex, marrow
Cell, endosperm etc.) or cell (such as megaspore, microspore, body cell) and protoplast, in sterile and suitable artificial training
Under the conditions of supporting base and illumination, temperature etc. manually, callus, adventitious bud, adventitious root are induced, complete plant is eventually formed.
Tissue culture procedures have the shortcomings that time-consuming, to culture environment and to the professional high of technical staff.Also, tissue cultures
Seedling afterwards also needs to undergo the domestication culture of the 1-5 months, extends growing-seedling period.
Therefore, a kind of dendrobium candidum method for culturing seedlings of non-tissue culture formula is needed badly.
The content of the invention
In order to solve the above-mentioned technical problem, the present invention provides method for culturing seedlings and the cultivation side of a kind of live formula dendrobium candidum
Method.The method for culturing seedlings utilizes liver moss to carry out live formula nursery, it is to avoid the shortcoming of tissue culture；And by nursery side of studio
The seedling that method is obtained carries out implanting and cultivating together with liver moss and moss growth hypothallus, it is possible to increase dendrobium candidum is in cultivation
Survival rate.
The present invention is achieved by the following technical solutions：
Seeds of Dendrobium Candidum is directly seeded on liver moss by a kind of method for culturing seedlings of live formula dendrobium candidum, methods described,
The Seeds of Dendrobium Candidum completes seed germination and process of emerging in the liver moss；The moss growth is in moss growth matrix
On layer.
Further, Seeds of Dendrobium Candidum is configured to seed suspension liquid, the seed suspension liquid is uniformly sprayed onto tongue
Moss surface, carries out seedling management, so that germination rate is higher than 50%.
Further, the seedling management is specially：In first 30 days, covered from sprinkling seed suspension liquid above liver moss
Film, forms miniature seedling culture space, in the miniature seedling culture space, and it is 15-25 DEG C to control temperature, and envionmental humidity control is 65
~75%, intensity of illumination is less than 20,000 lx；
Spray after seed suspension liquid 30, be i.e. in the nursery later stage, tamed, so that dendrobium candidum seedling gradually adapts to later stage cultivation
Train environment.
Further, the moss growth hypothallus is bark, soil；
Further, when using soil as moss growth hypothallus when, adjust soil pH, make soil pH, 5-5.5 it
Between.
Further, the liver moss is from liver moss on woodland tree time liver moss, bark or the liver moss manually cultivated.
Further, when using the liver moss manually cultivated, the method for culturing seedlings also includes the plantation step of liver moss, liver moss
Plantation step be specially：Block liver moss, milk and water are put into mixer together to be stirred, liver moss seed liquor is obtained；Will
The liver moss seed liquor is uniformly splashed (daily there is provided the environmental condition of suitable moss growth on the moss growth hypothallus
Spraying, keeps moistening, 22-27 DEG C, dim light), can when coverage rate of the liver moss on the moss growth hypothallus is higher than 50%
For use as the nursery of dendrobium candidum.
Further, in the liver moss seed liquor, liver moss is in 0.1-0.5cm particle, is uniformly distributed；The block tongue
The weight ratio of moss, the milk and the water is 4-7：1：1.
A kind of cultural method of live formula dendrobium candidum, the cultural method applies the iron sheet stone that the method for culturing seedlings is obtained
Dry measure used in former times seedling, is used for ecologic planting pattern in facility bionic cultivation and/or woods by the dendrobium candidum seedling, iron sheet is taken before cultivation
During stem of noble dendrobium seedling, by the liver moss in the range of 3-10cm near dendrobium candidum seedling root and the moss growth matrix below liver moss
Layer takes out together, transplants into cultivation matrix, then carries out conventional cultivation maintenance, dendrobium candidum can be harvested after 1~2 year.
Wherein, facility bionic cultivation, i.e., using facilities such as glasshouse or plastic film sheds, with bark etc. for cultivation base
Matter, is equipped with the facilities such as sunshade net, spraying and irrigation, imitates a kind of cultural method that wild environment cultivates dendrobium candidum；The woods
Interior ecologic planting, mainly has cultivation under woods and tree living to grow nonparasitically upon another plant two kinds of original ecology cultivating pattern, mainly uses the natural ring in woods
Border conditioning, reaches the purpose of original ecology cultivating.
The advantageous effects of the present invention：
Method for culturing seedlings of the present invention, there is provided a kind of brand-new seedling raising mannerses, makes iron independent of tissue culture technique
Skin stem of noble dendrobium industry has broken away from the limitation of tissue-cultured seedling, is that the extensive expansion of dendrobium candidum industry provides the foundation.
Method for culturing seedlings of the present invention is simple to operation, to nurturing an environment and to technical staff almost without specific
Limitation, can be by large-scale promotion application.
Cultural method of the present invention, using method for culturing seedlings of the present invention, children when can farthest improve cultivation
The survival rate of seedling.
In method for culturing seedlings provided by the present invention, include a kind of cultural method of liver moss, this method is for for preparing tongue
The block liver moss of moss seed liquor requires relatively low, even if dead liver moss, as long as wherein containing the spore that can be survived, with regard to energy
Used.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is explained in further detail.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, it is not used to
Limit the present invention.
On the contrary, the present invention covers any replacement done in the spirit and scope of the present invention being defined by the claims, repaiied
Change, equivalent method and scheme.Further, in order that the public has a better understanding to the present invention, below to the thin of the present invention
It is detailed to describe some specific detail sections in section description.Part without these details for a person skilled in the art
Description can also understand the present invention completely.
Embodiment 1
Seeds of Dendrobium Candidum is directly seeded on liver moss by a kind of method for culturing seedlings of live formula dendrobium candidum, methods described,
The Seeds of Dendrobium Candidum completes seed germination and process of emerging in the liver moss；The moss growth is in moss growth matrix
On layer.
In the present embodiment, the moss growth hypothallus is bark；The liver moss is from liver moss on bark.In other realities
Apply in example, the moss growth is on the growth substrate layer based on bark wood chip.
Seeds of Dendrobium Candidum is directly seeded on liver moss, the Seeds of Dendrobium Candidum completes seed in the liver moss and sprouted
Bud and process of emerging；Specially：Seeds of Dendrobium Candidum is configured to seed suspension liquid, the seed suspension liquid is uniformly sprayed onto
Liver moss surface, carries out seedling management, so that germination rate is higher than 50%.The seedling management is specially：From sprinkling seed suspension liquid
Rise in first 30 days, in the top overlay film of liver moss, form miniature seedling culture space, in the miniature seedling culture space, it is 15- to control temperature
25 DEG C, envionmental humidity control is 65~75%, and intensity of illumination is less than 20,000 lx；Spray after seed suspension liquid 30, i.e., after nursery
Phase, conventional domestication process is carried out, so that dendrobium candidum seedling gradually adapts to later stage planting environment, after taming 20-30 days, that is, obtained
It is 30% to obtain dendrobium candidum germination rate in the dendrobium candidum seedling of suitable cultivation, the present embodiment.
A kind of cultural method of live formula dendrobium candidum, method for culturing seedlings described in described cultural method application the present embodiment is obtained
Dendrobium candidum seedling, by the dendrobium candidum seedling be used for facility emulational growth (facility bionic cultivation utilizes glass
The facility such as greenhouse or plastic film shed, with bark etc. for cultivation matrix, is equipped with the facilities such as sunshade net, spraying and irrigation, imitates
Wild environment cultivates a kind of cultural method of dendrobium candidum), when dendrobium candidum seedling is taken before cultivation, by dendrobium candidum seedling root
Liver moss near portion in the range of 3-10cm and the bark below liver moss take out together, transplant into cultivation matrix, then carry out
Conventional cultivation is conserved, and dendrobium candidum can be harvested after 1~2 year.
Embodiment 2
Seeds of Dendrobium Candidum is directly seeded on liver moss by a kind of method for culturing seedlings of live formula dendrobium candidum, methods described,
The Seeds of Dendrobium Candidum completes seed germination and process of emerging in the liver moss.
In the present embodiment, the moss growth hypothallus is soil；Adjust soil pH, make soil pH, 5-5.5 it
Between, the soil of the pH is adapted to the growth of liver moss.
The liver moss is from the liver moss manually cultivated.The plantation step of liver moss is specially：By block liver moss, milk and water one
Rise and be put into mixer and be stirred, obtain liver moss seed liquor；The liver moss seed liquor is uniformly splashed in the moss growth base
There is provided the environmental condition of suitable moss growth (spraying daily, keep moistening, 22-27 DEG C, dim light) on matter layer, treat liver moss in institute
When stating the coverage rate on moss growth hypothallus higher than 90%, the nursery of dendrobium candidum may be used as.In the liver moss seed liquor
In, liver moss is in 0.3cm particle, is uniformly distributed；The weight ratio of the block liver moss, the milk and the water is 5：1：1.
Seeds of Dendrobium Candidum is directly seeded on liver moss, the Seeds of Dendrobium Candidum completes seed in the liver moss and sprouted
Bud and process of emerging.Specially：Seeds of Dendrobium Candidum is configured to seed suspension liquid, the seed suspension liquid is uniformly sprayed onto
Liver moss surface, carries out seedling management, and the seedling management is specially：From sprinkling seed suspension liquid in first 30 days, in liver moss
Top overlay film, forms miniature seedling culture space, in the miniature seedling culture space, and it is 25 DEG C to control temperature, envionmental humidity control
For 65%, intensity of illumination is 1.8 ten thousand lx；
Spray after seed suspension liquid 30, i.e. the nursery later stage, conventional domestication process is carried out, so that dendrobium candidum seedling is gradually
Later stage planting environment is adapted to, after taming 20-30 days, that is, iron sheet stone in the dendrobium candidum seedling of suitable cultivation, the present embodiment is obtained
Dry measure used in former times germination rate is 80%.
A kind of cultural method of live formula dendrobium candidum, the cultural method applies the iron sheet stone that the method for culturing seedlings is obtained
Dry measure used in former times seedling, the dendrobium candidum seedling is used for into ecologic planting pattern in woods, and (ecologic planting, mainly there is cultivation under woods in the woods
Grown nonparasitically upon another plant two kinds of original ecology cultivating pattern with tree living, mainly use the natural environmental condition in woods to act on, reach original ecology cultivating
Purpose.), when dendrobium candidum seedling is taken before cultivation, by the liver moss in the range of 3-10cm near dendrobium candidum seedling root with
And the moss growth hypothallus below liver moss takes out together, transplant into cultivation matrix, then carry out conventional cultivation maintenance, 1~2
Dendrobium candidum can be harvested after year.
Claims (9)
1. a kind of method for culturing seedlings of live formula dendrobium candidum, it is characterised in that methods described directly sows Seeds of Dendrobium Candidum
Onto liver moss, the Seeds of Dendrobium Candidum completes seed germination and process of emerging in the liver moss；The moss growth is in tongue
On moss growth substrate layer.
2. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 1, it is characterised in that by Seeds of Dendrobium Candidum
Seed suspension liquid is configured to, the seed suspension liquid is uniformly sprayed onto liver moss surface, seedling management is carried out, so that germination rate is high
In 50%.
3. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 2, it is characterised in that the seedling management tool
Body is：From sprinkling seed suspension liquid in first 30 days, in the top overlay film of liver moss, miniature seedling culture space is formed, miniature is educated at this
In seedling space, it is 15-25 DEG C to control temperature, and envionmental humidity control is 65~75%, and intensity of illumination is less than 20,000 lx；
Spray after seed suspension liquid 30, be i.e. in the nursery later stage, tamed, so that dendrobium candidum seedling gradually adapts to later stage cultivation ring
Border.
4. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 1, it is characterised in that the moss growth base
Matter layer is bark or soil.
5. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 1, it is characterised in that when using soil conduct
During moss growth hypothallus, soil pH is adjusted, makes soil pH, between 5-5.5.
6. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 1, it is characterised in that the liver moss selects woods
Between set lower liver moss, liver moss or the liver moss manually cultivated on bark.
7. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 6, it is characterised in that when using artificial culture
Liver moss when, the method for culturing seedlings also includes the plantation step of liver moss, and the plantation step of liver moss is specially：By block liver moss, ox
Milk and water are put into mixer and are stirred together, obtain liver moss seed liquor；The liver moss seed liquor is uniformly splashed in the tongue
There is provided the environmental condition of suitable moss growth on moss growth substrate layer, covering of the liver moss on the moss growth hypothallus is treated
When rate is higher than 50%, the nursery of dendrobium candidum may be used as.
8. a kind of method for culturing seedlings of live formula dendrobium candidum according to claim 7, it is characterised in that in the liver moss seed
In liquid, liver moss is in 0.1-0.5cm particle, is uniformly distributed；The weight ratio of the block liver moss, the milk and the water is 4-
7：1：1.
9. a kind of cultural method of live formula dendrobium candidum, one of described cultural method application claim 1-8 nursery sides
The dendrobium candidum seedling that method is obtained, it is characterised in that the dendrobium candidum seedling is used for facility bionic cultivation and/or Lin Neisheng
State cropping pattern, when dendrobium candidum seedling is taken before cultivation, by the liver moss in the range of 3-10cm near dendrobium candidum seedling root
And the moss growth hypothallus below liver moss takes out together, transplant into cultivation matrix, then carry out conventional cultivation maintenance, 1
Dendrobium candidum can be harvested after~2 years.
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Cited By (2)
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| CN111387016A (en) * | 2020-03-17 | 2020-07-10 | 浙江省林业科学研究院 | Preparation method of special foam matrix material for cultivation and cultivation method |
| CN111616038A (en) * | 2020-05-20 | 2020-09-04 | 龙陵县石斛研究所 | Dendrobium devonianum seedling growing method |
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