CN103270927B - Culture substrate for dendrobium candidum tissue-cultured seedlings - Google Patents
Culture substrate for dendrobium candidum tissue-cultured seedlings Download PDFInfo
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- CN103270927B CN103270927B CN201310225436.6A CN201310225436A CN103270927B CN 103270927 B CN103270927 B CN 103270927B CN 201310225436 A CN201310225436 A CN 201310225436A CN 103270927 B CN103270927 B CN 103270927B
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Abstract
The invention provides a culture substrate for dendrobium candidum tissue-cultured seedlings. The culture substrate comprises a first substrate layer, a second substrate layer, a third substrate layer and a fourth substrate layer from top to bottom sequentially, wherein the first substrate layer is formed by mixing laying of one or two kinds of crushed wood shavings and crushed bark, the second substrate layer is formed by laying of a plurality of small wood blocks, the third substrate layer is formed by laying of a plurality of long wood strips, and the fourth substrate layer is formed by laying of a plurality of crushed stones. According to the culture substrate for the dendrobium candidum tissue-cultured seedlings, the first substrate layer, the second substrate layer, the third substrate layer and the fourth substrate layer are arranged from top to bottom sequentially, and the substrate layers are made of different materials, so that the culture substrate for the dendrobium candidum tissue-cultured seedlings is low in pathogenic microorganism content and high in air permeability, seedling hardening is not required to be conducted when the substrate is used for culturing the dendrobium candidum tissue-cultured seedlings, and the survival rate of cultivation is high.
Description
Technical field
The present invention relates to a kind of candidum tissue culturing seedling cultivation matrix.
Background technology
Dendrobium candidum, claims again ribbed hedyotis herb, belongs to the orchid family epiphytic orchid class, is traditional rare traditional Chinese medicine, among the people being described as " first of Chinese nine immortal grass ".In < < Sheng Nong's herbal classic > >, be listed in top gradely, there is nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, relieving cough and moistening lung, the improving eyesight that wets one's whistle, improve human immunity, anti-curing oncoma and angiocardiopathy and improve sleep, prevent and treat cataract, the effect such as promote longevity.
The seed of dendrobium candidum is very tiny, and embryonic development is incomplete, and without endosperm tissue, under nature, germination rate is extremely low.In addition predatory excavating for a long time, causes wild dendrobium candidum resource closely exhausted, in Critical Condition, can not meet domestic and international market demand far away, causes market supply in short supply.Therefore, artificial planting dendrobium candidum industry develops rapidly, dendrobium candidum is planted seedling used substantially from plant tissue culture technique, yet, the group training seedling that Plant Tissue Breeding obtains is owing to being grown in for a long time tissue culture bottle, adaptive capacity is to external world low, and after plantation, lethality is often higher, so having relatively high expectations to cultivation matrix.Good cultivation matrix can increase a survival rate, reduces plantation cost.The problems referred to above are the problems that should pay attention to and solve in the design of candidum tissue culturing seedling cultivation matrix and use procedure.
Summary of the invention
The object of this invention is to provide a kind of candidum tissue culturing seedling cultivation matrix and solve group training seedling that Plant Tissue Breeding obtains owing to being grown in for a long time tissue culture bottle, adaptive capacity is to external world low, lethality higher problem often after plantation.
Technical solution of the present invention is:
A kind of candidum tissue culturing seedling cultivation matrix, comprise the hypothallus one, hypothallus two, hypothallus three and the hypothallus four that set gradually from top to bottom, described hypothallus one adopts one or both mixing of broken wood shavings, broken bark to lay and forms, described hypothallus two is that some wood particles are laid formation, described hypothallus three is that some long stuffs are laid formation, and described hypothallus four is that some rubbles are laid formation.
Preferably, the length of the wood particle of described hypothallus two is that 1 ~ 10cm, width are that 1 ~ 10cm, thickness are 0.5 ~ 5cm.
Preferably, the length of the long stuff of described hypothallus three is that 15 ~ 150cm, width are that 1 ~ 20cm, thickness are 0.5 ~ 5cm.
Preferably, the volume size of the rubble of described hypothallus four is 0.3 ~ 25cm
3.
Preferably, the thickness of described hypothallus one is 0.5-5cm, and the thickness of described hypothallus two is 5-15cm, and the thickness of described hypothallus three is 3-8cm, and the thickness of described hypothallus four is 3-20cm.
Preferably, the wood particle of the broken wood shavings of described hypothallus one or broken bark, hypothallus two, the long stuff of hypothallus three all by the immersion method of being exposed to the sun, make.
Preferably, the wood particle of the broken wood shavings of described hypothallus one or broken bark, hypothallus two, the long stuff of hypothallus three by pine tree, cypress and/or Chinese fir, made.
A kind of candidum tissue culturing seedling cultivation matrix of the present invention, by the hypothallus one setting gradually from top to bottom, hypothallus two, hypothallus three and hypothallus four, described hypothallus one adopts broken wood shavings, one or both of broken bark mix lays formation, described hypothallus two is that some wood particles are laid formation, described hypothallus three is that some long stuffs are laid formation, described hypothallus four is that some rubbles are laid formation, the pathogenic microorganism content that can guarantee this kind of candidum tissue culturing seedling cultivation matrix is few, gas permeability is strong, utilize this substrate culture candidum tissue culturing seedling without carrying out hardening, and plantation survival rate is high.
This kind of candidum tissue culturing seedling cultivation matrix, the wood particle of the broken wood shavings of hypothallus one or broken bark, hypothallus two, the long stuff of hypothallus three are all made by the immersion method of being exposed to the sun, the immersion method of being exposed to the sun soaks 24 ~ 48 hours in disinfectant or clear water, under the sun, be exposed to the sun 48 ~ 96 hours again, carry out sterilization, thereby increased the survival rate of candidum tissue culturing seedling plantation.This kind of candidum tissue culturing seedling cultivation matrix, gas permeability, moisture capacity are better, and the cultivation of suitable dendrobium candidum has realized that dendrobium candidum is efficient, the plantation demand of high yield.
The invention has the beneficial effects as follows: a kind of candidum tissue culturing seedling cultivation matrix of the present invention, by the hypothallus one, hypothallus two, hypothallus three and the hypothallus four that set gradually from top to bottom, and the selection of each layer of matrix is different, the pathogenic microorganism content that can guarantee this kind of candidum tissue culturing seedling cultivation matrix is few, gas permeability is strong, utilize this substrate culture candidum tissue culturing seedling without carrying out hardening, and plantation survival rate is high.In this kind of candidum tissue culturing seedling cultivation matrix, the wood particle of the broken wood shavings of hypothallus one or broken bark, hypothallus two, the long stuff of hypothallus three are all made by the immersion method of being exposed to the sun, and matrix is carried out can effectively increasing the survival rate that candidum tissue culturing seedling is planted after sterilization.This kind of candidum tissue culturing seedling cultivation matrix, gas permeability, moisture capacity are better, and the cultivation of suitable dendrobium candidum has realized that dendrobium candidum is efficient, the plantation demand of high yield.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment of the present invention;
Wherein: 1-hypothallus one, 2-hypothallus two, 3-hypothallus three, 4-hypothallus four.
Embodiment
Below in conjunction with accompanying drawing, describe the preferred embodiments of the present invention in detail.
As shown in Figure 1, the present embodiment provides a kind of candidum tissue culturing seedling cultivation matrix, comprise the hypothallus 1, hypothallus 22, hypothallus 33 and the hypothallus 44 that set gradually from top to bottom, described hypothallus 1 adopts broken wood shavings, broken bark to mix and lays formation, described hypothallus 22 is that some wood particles are laid formation, described hypothallus 33 is that some long stuffs are laid formation, and described hypothallus 44 is that some rubbles are laid formation.The thickness of described hypothallus 1 is 0.5-5cm, is preferably 2cm, and the thickness of described hypothallus 22 is 5-15cm, is preferably 10cm, and the thickness of described hypothallus 33 is 3-8cm, is preferably 5cm, and the thickness of described hypothallus 44 is 3-20cm, is preferably 5cm.
The length of the wood particle of described hypothallus 22 is 1 ~ 10cm, and preferably 3 ~ 8cm, width are 1 ~ 10cm, and preferably 2 ~ 6cm, thickness are 0.5 ~ 5cm, preferably 1 ~ 2cm.The length of the long stuff of described hypothallus 33 is 15 ~ 150cm, and preferably 30 ~ 100cm, width are 1 ~ 20cm, and preferably 4 ~ 10cm, thickness are 0.5 ~ 5cm, preferably 1 ~ 2cm.The volume size of the rubble of described hypothallus 44 is 0.3 ~ 25cm
3, preferred 0.5 ~ 15 cm
3.The wood particle of the broken wood shavings of described hypothallus 1 or broken bark, hypothallus 22, the long stuff of hypothallus 33 are all made by the immersion method of being exposed to the sun.The wood particle of the broken wood shavings of described hypothallus 1 or broken bark, hypothallus 22, the long stuff of hypothallus 33 are made by cypress.
A kind of candidum tissue culturing seedling cultivation matrix of the present embodiment, by the hypothallus 1 setting gradually from top to bottom, hypothallus 22, hypothallus 33 and hypothallus 44, described hypothallus 1 adopts broken wood shavings, formation is laid in mixing of broken bark, described hypothallus 22 is that some wood particles are laid formation, described hypothallus 33 is that some long stuffs are laid formation, described hypothallus 44 is that some rubbles are laid formation, the pathogenic microorganism content that can guarantee this kind of candidum tissue culturing seedling cultivation matrix is few, gas permeability is strong, utilize this substrate culture candidum tissue culturing seedling without carrying out hardening, and plantation survival rate is high.
This kind of candidum tissue culturing seedling cultivation matrix, the wood particle of the broken wood shavings of hypothallus 1 or broken bark, hypothallus 22, the long stuff of hypothallus 33 are all made by the immersion method of being exposed to the sun, the immersion method of being exposed to the sun soaks 24 ~ 48 hours in disinfectant or clear water, under the sun, be exposed to the sun 48 ~ 96 hours again, carry out sterilization, thereby increased the survival rate of candidum tissue culturing seedling plantation.This kind of candidum tissue culturing seedling cultivation matrix, gas permeability, moisture capacity are better, and the cultivation of suitable dendrobium candidum has realized that dendrobium candidum is efficient, the plantation demand of high yield.
In order further to determine that the matrix of the present embodiment is used for cultivating the effect of candidum tissue culturing seedling, has done following test.Experimental group matrix is the present embodiment matrix, and the experiment matrix of control group 1,2,3,4 adopts wood chip matrix, loose squama matrix, pearlite interstitial substance, river sand matrix successively.Except matrix difference, candidum tissue culturing seedling growing way situation is similar, and cultivation management, culture environment condition etc. are identical, incubation time from April to June, observation analysis growing state, result of the test is as shown in table 1.As shown in Table 1, the survival rate of the dendrobium candidum that the survival rate of the dendrobium candidum that the matrix of the present embodiment is cultivated is cultivated apparently higher than four groups of control group matrix, and average every strain increases the dendrobium candidum that biomass is cultivated apparently higher than four groups of control group matrix.
The comparative result of the matrix of table 1 the present embodiment and the matrix of control group plantation dendrobium candidum situation
| ? | Different substrates | Survival rate (%) | Average every strain increases biomass (g) |
| Experimental group | The matrix of the present embodiment | 98.65 | 0.06 |
| Control group 1 | Wood chip matrix | 52.86 | -0.04 |
| Control group 2 | Pine squama matrix | 65.77 | -0.08 |
| Control group 3 | Pearlite interstitial substance | 72.56 | -0.02 |
| Control group 4 | River sand | 44.72 | -0.12 |
Claims (2)
1. a candidum tissue culturing seedling cultivation matrix, it is characterized in that: comprise the hypothallus one (1), hypothallus two (2), hypothallus three (3) and the hypothallus four (4) that set gradually from top to bottom, described hypothallus one (1) adopts one or both mixing of broken wood shavings, broken bark to lay and forms, described hypothallus two (2) is that some wood particles are laid formation, described hypothallus three (3) is that some long stuffs are laid formation, and described hypothallus four (4) is that some rubbles are laid formation; The length of the wood particle of described hypothallus two (2) is that 1 ~ 10cm, width are that 1 ~ 10cm, thickness are 0.5 ~ 5cm; The length of the long stuff of described hypothallus three (3) is that 15 ~ 150cm, width are that 1 ~ 20cm, thickness are 0.5 ~ 5cm; The thickness of described hypothallus one (1) is 0.5-5cm, and the thickness of described hypothallus two (2) is 5-15cm, and the thickness of described hypothallus three (3) is 3-8cm, and the thickness of described hypothallus four (4) is 3-20cm; The long stuff of the wood particle of the broken wood shavings of described hypothallus one (1) or broken bark, hypothallus two (2), hypothallus three (3) is all made by the immersion method of being exposed to the sun, the immersion method of being exposed to the sun soaks 24 ~ 48 hours in disinfectant or clear water, then under the sun, is exposed to the sun 48 ~ 96 hours; The long stuff of the wood particle of the broken wood shavings of described hypothallus one (1) or broken bark, hypothallus two (2), hypothallus three (3) is made by pine tree, cypress and/or Chinese fir.
2. candidum tissue culturing seedling cultivation matrix as claimed in claim 1, is characterized in that: the volume size of the rubble of described hypothallus four (4) is 0.3 ~ 25cm
3.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US11686021B2 (en) | 2014-06-29 | 2023-06-27 | Profile Products L.L.C. | Growing medium and mulch fiber opening apparatus |
| WO2016003901A1 (en) | 2014-06-29 | 2016-01-07 | Profile Products L.L.C. | Bark and wood fiber growing medium |
| US10889758B2 (en) | 2014-06-29 | 2021-01-12 | Profile Products, L.L.C. | Naturally dyed mulch and growing media |
| CN104429847B (en) * | 2014-10-24 | 2017-06-13 | 桂林丰润莱生物科技有限公司 | The method that dendrobium candidum is planted using the plant slag after effective component extracting |
| CN105104016A (en) * | 2015-08-11 | 2015-12-02 | 宁波德健生物科技有限公司 | Facility for planting Dendrobium officinale Kimura et Migo in photovoltaic power station and planting method of Dendrobium officinale Kimura et Migo |
| CN105766591A (en) * | 2016-04-25 | 2016-07-20 | 广西智宝科技有限公司 | Dendrobium officinale culture medium and preparation method thereof |
| CN105850682A (en) * | 2016-04-25 | 2016-08-17 | 广西智宝科技有限公司 | Special cultivation substrate for dendrobium officinale and preparation method of special cultivation substrate |
| CN105766592A (en) * | 2016-04-27 | 2016-07-20 | 覃伟 | Planting matrix for artificial planting of Caulis Dendrobii and preparation method thereof |
| CN106171888B (en) * | 2016-07-18 | 2020-01-31 | 福建连天福生物科技有限公司 | Dendrobium officinale culture medium and culture method |
| CN106561331A (en) * | 2016-10-27 | 2017-04-19 | 安徽省霍山县祥佑堂中药材开发有限公司 | Dendrobe culture substrate |
| CA3049419A1 (en) * | 2017-01-06 | 2018-07-12 | Profile Products L.L.C. | Bark and wood fiber growing medium |
| CN112616661A (en) * | 2020-12-16 | 2021-04-09 | 贵州务川科华生物科技有限公司 | Cultivation method of broussonetia papyrifera seedlings |
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| CN101336605B (en) * | 2007-07-07 | 2011-07-20 | 赤水市信天中药产业开发有限公司 | Dendrobium nobi tissue culturing and planting method |
| CN101564008B (en) * | 2008-04-24 | 2011-03-23 | 中国医学科学院药用植物研究所 | Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings |
| CN101352140B (en) * | 2008-08-22 | 2010-09-29 | 李明焱 | Dendrobium officinale substrate and planting method |
| CN102124936B (en) * | 2010-12-10 | 2012-05-30 | 梁经军 | Imitated wild planting matrix of Dendrobium officinale Kimura et Migo |
| CN102668958A (en) * | 2012-06-08 | 2012-09-19 | 普洱市思茅区五彩花木专业合作社 | Culture matrix and preparation method thereof for dendrobium |
| CN103102209A (en) * | 2012-09-28 | 2013-05-15 | 广东永生源生物科技有限公司 | Culture substrate for high density planting of dendrobium officinale with high yield |
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