CN1709023A - Inoculating method for increasing ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases - Google Patents
Inoculating method for increasing ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases Download PDFInfo
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- CN1709023A CN1709023A CN 200510083853 CN200510083853A CN1709023A CN 1709023 A CN1709023 A CN 1709023A CN 200510083853 CN200510083853 CN 200510083853 CN 200510083853 A CN200510083853 A CN 200510083853A CN 1709023 A CN1709023 A CN 1709023A
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Abstract
The invention utilizes artificial regulation and control measures to change inoculation mode and method. Said invention uses the mixture of turf undergone the process of 10KGy radiation sterilization or 120dec.C high-pressure steam sterilization for 2 hr. and vermiculite (2:1-4:3) as matrix. The ratio of matrix and inoculation agent is 10:1-15:1, it can ensure that single plant has 50-80 spores. At the time of cultivating seedling and sowing the inoculation agent and the matrix can be mixed, and the mixing mode is divided into two kinds of that the inoculation agent and matrix are completely mixed or only the inoculation agent is applied in sowing position.
Description
Affiliated technical field
The invention belongs to the microorganism technique for applying, be specifically related to promote mycorrhizal fungi and plant symbiotic relation, set up and improve plant disease resistance method.
Background technology
Cucumber is one of main vegetable species in the facility cultivation, it occupies critical role in the year-round supply of China vegetables, according to incompletely statistics, kind surplus the disease 50 is arranged in the cucumber cultivation, take place throughout the year and cause disease than heavy losses have 10 surplus kind of soil-borne disease, invade the parasitic host plant by germ from young root or wound, become the greatest factor of restriction vegetables production development, throughout the year the incidence of disease is generally 10%-30%, when serious even total crop failure.Chemical agent is the leading approach of soil-borne disease control at present, but use the caused pathogen pesticide resistance of chemical agent to strengthen for a long time and make people's clear understanding to its risk the pollution problem of environment, take the non-environmental pollution control strategy with suiting measures to local conditions, economical, safety, prevent and treat cucumber disease effectively, guarantee its good quality and high output, realize that the sustainable cultivation of cucumber is very necessary.Can when reducing the chemicals input, take into account the non-harmful bio-control method of sustainable development of agriculture to become the focus of concern.
Studies show that most of vegetable crops all can with arbuscular mycorrhiza (arbuscular mycorrhiza, AM) fungi forms stable syntaxial system, can increase the absorption of plant to mineral nutrition, particularly the absorbing capacity to phosphorus strengthens, also improve the plant drought ability simultaneously, improve the tolerance of plant, the structure of can also improving the soil adverse circumstance such as hot and cold, heavy metal pollution etc., it is pollution-free, nonhazardous, is the desirable preparation of producing pollution-free food.The mycorhiza technology is as an effective and feasible biological pathway, can excavate biological self potentiality, improves the utilize ability of plant to natural resources, thereby cuts down the consumption of energy, and alleviates the pressure that environment bears, thereby wide application prospect is arranged.There are some researches show of improvement or the damage compensation of inoculation AM fungi simultaneously by the plant corpus nutrition condition, the limited competition of infecting the site of host's photosynthetic product and root system, the change of rhizosphere microflora and to the effects such as activation of resistance mechanism in the plant corpus, improved the resistance of vegetable crop to pathogen (fungal disease, nematode), the inoculation arbuscular mycorrhizal fungi is the bio-control method with higher application prospect.
The arbuscular mycorrhizal fungi biotechnology provides a new way of taking into account economic benefit and environmental benefit for overcoming soil-borne disease, but reasonably by kind method, inoculate matrix efficiently and still lack enough research, for the situation that infects that how to improve the AM fungi, improve disease resisting effect by artificial mode and on concrete implementation method, remain with further perfect.
Summary of the invention
Technical solution of the present invention provides a kind of method that improves the performance of the anti-soil-borne disease of cucumber, and main the employing by to its inoculation arbuscular mycorrhizal fungi promotes infecting of mycorrhizal fungi to realize by the technological means regulation and control.
The method of described enhancing plant disease-resistant performance, be based on the foundation of symbiotic relation between plant and the mycorrhizal fungi, change the vaccination ways method, utilize rational matrix by artificial regulation measure, the structure Mycorrhizal is good, and the strong plant of healthy and strong disease-resistant performance is to satisfy the demand of producing.
The Inoculant that method adopted of described enhancing plant disease-resistant performance obtains from the mycorrhizal fungi microbial inoculum production unit of providing, and is the mixture of the root segment that contains fungal spore and mycelia and infected.
The method of described enhancing plant disease-resistant performance, matrix is the peat composed of rotten mosses and vermiculite (2: 1～4: 3) mixture, and is through 10KGy irradiation sterilization or 121 ℃ of high pressure steam sterilizations 2 hours, standby behind the natural air drying.Matrix and Inoculant ratio are 10: 1～15: 1, guarantee 50-80 spore of individual plant.When seeding and growing seedling, it is mixed to be about to Inoculant and matrix phase.Mixed mode is divided into the complete mixing of matrix or only Inoculant is applied to two kinds at sowing position.Arbuscular mycorrhizal fungi is promoted inoculation when the seeding and growing seedling as a kind of biological control technology in Mycorrhizal matrix; make full use of this production technology of seedling raising and transplanting in the production of facilities horticulture vegetables; regulating suitable moisture and illumination regulates; treat plant strain growth after 4～5 weeks, the Mycorrhizal plant that can obtain efficient syntaxial system is used for land for growing field crops or the cultivation of protection ground.
Description of drawings
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Embodiment
Cucumber is green No. 3 of Tianjin, and seed is through 10%H2O2 surface sterilization 5min, and the 2h that hots water treatment of seeds after clean with the distilled water flushing of sterilization is put in 28 ℃ of dark vernalization on the wet filter paper, chooses the planting seed of homogeneous when waiting nearly 1cm of plumule.
The peat composed of rotten mosses and vermiculite mix the back with 1: 1 volume ratio, and intermittently moist heat sterilization 2h is standby.
Nutritive cube (going up bore 8.5cm, footpath, end 6cm, high 7.5cm) seals with 3% formaldehyde and 1 week carried out surface sterilization, treats that smell is standby after dispersing.
Inoculant is Glomus etunicatum (China Agricultural University separates, the European numbering BEG168 of DSMZ), with the mixture of the root segment that contains fungal spore and mycelia and infected as Inoculant.
Simulate soil-borne disease with the cucumber fusarium axysporum pathogen: Fusarium oxysporum (F.o) is provided by Plant Protection institute, Chinese Academy of Agricultral Sciences.Get pathogen F.o at potato (Potato DextroseAgar, PDA) cultivated 4 days on the medium, cultivated for 2 weeks from colony edge picking 3-4mm bacterium piece 25 ℃, 50rpm in the PDA liquid nutrient medium then, double gauze with sterilization filters mycelia, with 4 ℃ of remaining culture fluids, centrifugal 3 times of 9000rpm, collect spore suspension, blood cell plate counting is regulated spore concentration to 1 * 105 piece/ml with 10mmol/L MgSO47H2O at last.
Every basin is packed into through the 180g of abundant mixing seedling medium and 15gAM microbial inoculum, and the contrast of not inoculating then adds the sterilization microbial inoculum of equivalent and the bacterial classification filtrate 10ml that filters through double-deck filter paper, to guarantee the uniformity of other microbe species.2 of every basin sowings, final singling one strain of emerging.
Pathogen inoculation: behind the plant strain growth 28d, adopt root-pouring method inoculation pathogen F.o spore suspension 10ml/ basin.Control treatment replaces with the equivalent aqua sterilisa.
Inoculate arbuscular mycorrhizal fungi at seeding time, and behind plant strain growth 28d, inoculate pathogen and handle.Blank is established in test, single inoculation AM fungi, single inoculation pathogen, 4 processing such as the two inoculations of AM fungi and pathogen.Each is handled and repeats 16 times, and completely random is arranged.
After planting growth regulation is 28 days, the mycorhiza infection rate can reach 40%, formed good cogeneration system with plant, after the soil-borne disease simulate growth 14 days, do not inoculate pathogen processing plant and do not have morbidity trend, yellow and wilting have appearred in the processing of single inoculation pathogen, incidence constantly rises, the Mycorrhizal plant is handled acomia symptom condition, has effectively reduced the incidence of disease and disease index, has reduced the harm of cucumber fusarium axysporum.
Upgrowth situation index such as leaf area and the dry weight etc. of plant, all there were significant differences than contrast for the Mycorrhizal plant, the result shows that the inoculation arbuscular mycorrhizal fungi has improved effective photosynthesis area, promote growing of plant, increased the accumulation of dry matter, research simultaneously shows that also the inoculation arbuscular mycorrhizal fungi can effectively promote plant to phosphorus, the absorption of nutritive elements such as copper and utilization, thus improved the disease-resistant performance of plant.
Claims (8)
1. inoculation method that improves ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases, this method comprise to be made cucumber inoculation arbuscular mycorrhizal fungi, and artificial mode induces and promote that mycorhiza infects, and improves features such as plant disease-resistant performance.
2. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 1, it is characterized in that, described Inoculant is for containing arbuscular mycorrhizal fungi spore, mycelial bacterium soil, there is the bacterial classification of positive effect of inoculation to be: Glomus etunicatum (China Agricultural University separates, the European numbering BEG168 of DSMZ).
3. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 1 is characterized in that, artificial mode promotes mycorhiza to infect, improve the plant disease-resistant performance and comprise the collocation of selection (1) matrix, (2) inoculum concentration, (3) are by kind of a time, (4) vaccination ways.
4. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 3, it is characterized in that, the collocation of described (1) matrix, be mixed, through 10KGy irradiation sterilization or 121 ℃ of high pressure steam sterilizations 2 hours at the inoculation peat composed of rotten mosses that adopts of mycorrhizal fungi and vermiculite (2: 1～4: 3).
5. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 3 is characterized in that, described (2) inoculum concentration refers to that matrix and Inoculant ratio are 10: 1～15: 1, guarantees 50-80 spore of individual plant.
6. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 3 is characterized in that, described (3) inoculation time is sneaked in the matrix during for sowing.
7. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 3 is characterized in that, described (4) vaccination ways comprises with the complete mixing of matrix or only Inoculant is applied to and sows the position.
8. the inoculation method of raising ramaria mycorrhizal fungi effect for preventing and treating cucumber soil-infectious diseases according to claim 1 is cultivated in culturing room or greenhouse, keeps moisture 18～20% in the incubation through 4～5 weeks, can obtain to be equipped with to transplant to use seedling.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186542B (en) * | 2007-12-11 | 2011-04-13 | 云南神宇新能源有限公司 | Jatropha curcas mycorhiza bacterium composite fertilizer and preparation method thereof |
| CN102523863A (en) * | 2011-11-18 | 2012-07-04 | 中国中医科学院中药研究所 | Method for cultivating Panax notoginseng by arbuscular mycorrhizal fungi technique |
| CN103125353A (en) * | 2011-11-18 | 2013-06-05 | 中国中医科学院中药研究所 | Biological control method to improve disease-resistance of cultured traditional Chinese herbal medicines |
| CN114451217A (en) * | 2022-03-15 | 2022-05-10 | 商丘师范学院 | Arbuscular mycorrhizal fungus cultivation method and method for improving growth and disease resistance of hot pepper |
| CN114532145A (en) * | 2022-03-09 | 2022-05-27 | 河北建设集团安装工程有限公司 | Propagation method of arbuscular mycorrhizal fungal inoculant for desertified degraded sand soil |
-
2005
- 2005-07-14 CN CN 200510083853 patent/CN1709023A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186542B (en) * | 2007-12-11 | 2011-04-13 | 云南神宇新能源有限公司 | Jatropha curcas mycorhiza bacterium composite fertilizer and preparation method thereof |
| CN102523863A (en) * | 2011-11-18 | 2012-07-04 | 中国中医科学院中药研究所 | Method for cultivating Panax notoginseng by arbuscular mycorrhizal fungi technique |
| CN103125353A (en) * | 2011-11-18 | 2013-06-05 | 中国中医科学院中药研究所 | Biological control method to improve disease-resistance of cultured traditional Chinese herbal medicines |
| CN103125353B (en) * | 2011-11-18 | 2014-11-05 | 中国中医科学院中药研究所 | Biological control method to improve disease-resistance of cultured traditional Chinese herbal medicines |
| CN114532145A (en) * | 2022-03-09 | 2022-05-27 | 河北建设集团安装工程有限公司 | Propagation method of arbuscular mycorrhizal fungal inoculant for desertified degraded sand soil |
| CN114451217A (en) * | 2022-03-15 | 2022-05-10 | 商丘师范学院 | Arbuscular mycorrhizal fungus cultivation method and method for improving growth and disease resistance of hot pepper |
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