CN114303856A - Drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting - Google Patents
Drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting Download PDFInfo
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Abstract
The invention provides a drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting, which belongs to the technical field of agriculture and comprises the following steps: s1, selecting a variety with tillering capacity more than equal; s2, transplanting the sugarcane tissue-cultured rooted seedlings into a seedling container for culturing, hardening the seedlings and transplanting the seedlings into single seedlings with independent root systems in a single-plant mode after cluster planting and rooting; s3, spraying a growth regulator to the single seedlings to control the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method; s4, outplanting when the seedlings grow to 10-12 leaves, controlling water and fertilizer 5-7 days before outplanting, and spraying tillering-promoting medicines. The invention adopts the tissue culture seedling substrate mesh bag technology, overcomes the defect of false planting seedling cultivation in soil substrate plastic nutrition hole tray culture, improves the seedling quality, shortens the seedling time, reduces the transportation and planting cost, has uniform specification of the tissue culture seedling substrate, and is suitable for mechanical automatic seeding.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting.
Background
Sugarcane is an important sugar crop in China. The virus-free healthy tissue culture seedlings of the sugarcane are based on the technical principle of tissue culture, seedlings are bred in an industrial production mode under manual control, the breeding speed is high, the production time is not limited, the seedlings can be planted at any time and any place, the variation of the characters of the sugarcane seedlings is small, the virus-free healthy tissue culture seedlings are healthy, the occupied area is small, and the virus-free healthy tissue culture seedlings are the basis for the propagation, the qualitative purification and the rejuvenation of new varieties of the sugarcane. However, because the sugarcane tissue culture seedlings are weak and small, the root system absorption capacity is poor, the stress resistance is not strong, the sugarcane tissue culture seedlings cannot be directly planted in the field, and when the sugarcane tissue culture seedlings are transplanted to the field from a tissue culture laboratory, the sugarcane tissue culture seedlings need to be subjected to seedling hardening, cluster planting and temporary planting stages and then transplanted to the field for planting. The traditional temporary planting seedling raising is cultivated by a nutrition bag, a nutrition plate, a biodegradable seedling raising cup and the like, and can be transplanted to a field for planting after 30-40 days of nursery growth. The light-weight matrix mesh bag is widely applied to seedling raising of eucalyptus tissue culture seedlings, and the bred false-planted eucalyptus tissue culture seedlings have the advantages of developed root systems, good air root pruning effect, more lateral roots, no seedling revival period, long seedling storage time, contribution to soil improvement, reduction of seedling transportation and afforestation cost, improvement of afforestation survival rate, enhancement of wind resistance and the like. Eucalyptus belongs to woody plants, sugarcane belongs to gramineae, and no report is found if the effect of tissue culture seedlings according to trees can be achieved by breeding sugarcane tissue culture seedlings by using light matrix mesh bags. At present, the sugarcane tissue culture seedlings mainly adopt plastic hole trays and adopt yellow soil as a matrix to cultivate the false-planted seedlings, the labor intensity of seedling outplanting and carrying is high and the efficiency is low due to the heavy weight of the used matrix, and the seedling pulling, basket loading, carrying and seedling taking processes are easy to cause the damage of the roots of the seedlings and the mud dropping to form bare-rooted seedlings, so that the early growth of the seedlings is not facilitated. The light matrix mesh bag seedling raising technology can avoid the defects.
Disclosure of Invention
The invention aims to provide a drought-tolerant sugarcane tissue culture seedling culture method suitable for mechanical planting, which adopts a tissue culture seedling matrix mesh bag technology, combines the sugarcane tissue culture seedling culture reality and carries out tissue culture seedling temporary planting and cultivation by using a tissue culture seedling matrix mesh bag container, overcomes the defect of soil matrix plastic nutrition hole tray culture temporary planting seedling cultivation, improves the seedling quality, shortens the seedling culture time and reduces the transportation and planting cost, and the tissue culture seedling cultivated by using the mesh bag matrix has drought resistance, and does not need to be watered to fix roots during planting in spring.
The technical scheme of the invention is realized as follows:
the invention provides a drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting, which comprises the following steps:
s1, seed selection: selecting a variety with tillering force more than equal;
s2, single-plant temporary planting of sugarcane tissue culture rooted seedlings: transplanting the sugarcane tissue culture rooted seedlings into a seedling culture container for culture, hardening the seedlings and transplanting the seedlings into single seedlings with independent root systems by single plants after cluster planting and rooting;
s3, single-plant seedling temporary planting management: spraying a growth regulator on the single seedlings to control the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
s4, outplanting: outplanting when the seedling grows to 10-12 leaves, controlling water and fertilizer 5-7 days before outplanting, and spraying tillering promoting medicine.
As a further improvement of the invention, the variety comprises sugar cane and fruit cane.
As a further improvement of the invention, the seedling culture container is a cylindrical mesh bag filled with the tissue culture seedling substrate, the specification is that the diameter is 2-6cm, the height is 5-12cm, and the tissue culture seedling substrate is added.
As a further improvement of the invention, the tissue culture seedling substrate comprises 5-10 parts by weight of chaff, 12-15 parts by weight of straw, 2-5 parts by weight of plant ash and 3-7 parts by weight of waste bagasse.
As a further improvement of the invention, the matrix of the tissue culture seedlings comprises 7 parts by weight of chaff, 13 parts by weight of straws, 3 parts by weight of plant ash and 5 parts by weight of waste bagasse.
As a further improvement of the invention, the growth regulator comprises 5-10ppm of indoleacetic acid, 100-120ppm of paclobutrazol and 0.5-1 wt% of monopotassium phosphate.
As a further improvement of the invention, the spraying amount of the growth regulator is 14-17m per liter of the liquid medicine2And (5) nursery garden.
As a further improvement of the invention, the tillering-promoting drug comprises 150-200ppm compound sodium nitrophenolate and 2-4 wt% of urea.
As a further improvement of the invention, the spraying amount of the tillering-promoting medicine is 10-14m per liter of liquid medicine2And (5) nursery garden.
The invention has the following beneficial effects: the invention selects the variety with tillering force more than medium. The agent for promoting tillering is used for processing before outplanting, so as to achieve the effect of early tillering.
The tissue culture seedling matrix is loose, breathable and permeable, the root system grows quickly, the time of seedling emergence is 20 days earlier than that of traditional yellow soil seedling culture, the seedling culture time of a nursery is reduced, and the utilization rate of the nursery is improved. The light matrix mesh bag seedlings are planted in the field without a seedling revival period, the root systems directly extend to the periphery and downwards after planting, the growth vigor of the seedlings is superior to that of yellow soil plug seedlings, the growth period of the seedlings is long, the growth vigor is vigorous, and the group seedling culture of sugarcane can be widely popularized and planted.
The invention adopts the tissue culture seedling substrate mesh bag technology, combines the sugarcane tissue culture seedling culture reality and carries out tissue culture seedling temporary planting culture by using the tissue culture seedling substrate mesh bag container, overcomes the defect of the plastic nutrition hole tray culture temporary planting seedling culture of the soil substrate, and the tissue culture seedling cultured by using the mesh bag substrate has drought resistance, does not need to pour root fixing water during the spring planting, improves the seedling culture quality, shortens the seedling culture time, reduces the transportation and planting cost, has uniform specification of the tissue culture seedling culture substrate, and is suitable for mechanical automatic seeding. The seed with the seed coat does not damage the root system, greatly improves the survival rate of the tissue culture seedlings of the sugarcane, and provides theoretical basis and practical guidance for planting the sugarcane by using the tissue culture seedlings in a large area.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view showing the cultivation of a seedling using a mesh bag filled with a tissue culture substrate according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1, a drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method comprises the following steps:
s1, seed selection: selecting a variety with tillering force more than equal;
s2, single-plant temporary planting of sugarcane tissue culture rooted seedlings: transplanting the sugarcane tissue culture rooted seedlings into a seedling culture container for culture, hardening the seedlings and transplanting the seedlings into single seedlings with independent root systems by single plants after cluster planting and rooting; the seedling culture container is a mesh bag filled with a tissue culture seedling substrate, the diameter of the specification is 2cm, the height of the specification is 5cm, and the tissue culture seedling substrate is added; the tissue culture seedling substrate comprises 5g of rice husk, 12g of straws, 2g of plant ash and 3g of waste bagasse;
s3, single-plant seedling temporary planting management: spraying growth regulator to the single seedlings to control the height of the single seedlings before outplantingMaking the thickness of the product at 20-30 cm; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method; the growth regulator contains 5ppm of indoleacetic acid, 100ppm of paclobutrazol and 0.5 wt% of monopotassium phosphate, and the spraying amount is 14m per liter of liquid medicine2And (5) nursery garden.
S4, outplanting: outplanting when the seedling grows to 10-12 leaves, controlling water and fertilizer 5-7 days before outplanting, and spraying tillering promoting medicine. The tillering-promoting medicine comprises 150ppm compound sodium nitrophenolate and 2 wt% of urea, and the spraying amount is 10m per liter of liquid medicine2And (5) nursery garden.
Example 2
Referring to fig. 1, a drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method comprises the following steps:
s1, seed selection: selecting a variety with tillering force more than equal;
s2, single-plant temporary planting of sugarcane tissue culture rooted seedlings: transplanting the sugarcane tissue culture rooted seedlings into a seedling culture container for culture, hardening the seedlings and transplanting the seedlings into single seedlings with independent root systems by single plants after cluster planting and rooting; the seedling culture container is a mesh bag filled with a tissue culture seedling substrate, the diameter of the specification is 6cm, the height of the specification is 12cm, and the tissue culture seedling substrate is added; the tissue culture seedling substrate comprises 10g of rice husks, 15g of straws, 5g of plant ash and 7g of waste bagasse;
s3, single-plant seedling temporary planting management: spraying a growth regulator on the single seedlings to control the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method; the growth regulator contains 10ppm of indoleacetic acid, 120ppm of paclobutrazol and 1 wt% of monopotassium phosphate, and the spraying amount is 17m per liter of liquid medicine2And (5) nursery garden.
S4, outplanting: outplanting when the seedling grows to 10-12 leaves, controlling water and fertilizer 5-7 days before outplanting, and spraying tillering promoting medicine. The tillering-promoting medicine comprises 200ppm of compound sodium nitrophenolate and 4 wt% of urea, and the spraying amount is 14m per liter of liquid medicine2And (5) nursery garden.
Example 3
Referring to fig. 1, a drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method comprises the following steps:
s1, seed selection: selecting a variety with tillering force more than equal;
s2, single-plant temporary planting of sugarcane tissue culture rooted seedlings: transplanting the sugarcane tissue culture rooted seedlings into a seedling culture container for culture, hardening the seedlings and transplanting the seedlings into single seedlings with independent root systems by single plants after cluster planting and rooting; the seedling culture container is a mesh bag filled with a tissue culture seedling substrate, the diameter of the specification is 3cm, the height of the specification is 6cm, and the tissue culture seedling substrate is added; the tissue culture seedling substrate comprises 7g of rice husks, 13g of straws, 3g of plant ash and 5g of waste bagasse;
s3, single-plant seedling temporary planting management: spraying a growth regulator on the single seedlings to control the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method; the growth regulator contains 7ppm of indoleacetic acid, 110ppm of paclobutrazol and 0.7 wt% of monopotassium phosphate, and the spraying amount is 15m per liter of liquid medicine2And (5) nursery garden.
S4, outplanting: outplanting when the seedling grows to 10-12 leaves, controlling water and fertilizer 5-7 days before outplanting, and spraying tillering promoting medicine. The tillering-promoting medicine comprises 170ppm compound sodium nitrophenolate and 3 wt% of urea, and the spraying amount is 12m per liter of liquid medicine2And (5) nursery garden.
Test example 1
1. Materials and methods
1.1 test materials
1.1.1 tissue culture cluster-cultured rooted seedlings
The sugarcane variety clone is guilt No. 42, guilt No. 58 and guilt No. 1. After the cluster planting roots for one month, transplanting and culturing by single plant.
1.1.2 seedling raising container
Mesh bag with tissue culture seedling substrate: adding the uniformly mixed tissue culture seedling substrate into the non-woven fabric mesh bag, wherein the specification diameter is 3cm, and the height is 6 cm;
plastic hole trays: and (3) filling yellow mud into a plastic plug tray with the aperture of 5cm, the specification of 54cm multiplied by 33cm and the number of holes of 54.
1.2 test methods
1.2.1, respectively using a mesh bag (A) filled with a tissue culture seedling matrix and a plastic hole tray and adding yellow soil (CK) to carry out seedling culture, wherein the seedling culture method is the same as that of the embodiment 1, and then carrying out comparison tests on the survival rate of the temporary planting, the number of green leaves, the plant height and the root weight.
Selecting sand culture seedlings with relatively consistent sizes by adopting a random block design, repeating the steps for 3 times, counting the survival rate after the seedlings survive every 540 seedlings are repeated, and then randomly extracting 30 seedlings in each repetition to measure the number of green leaves, the plant height and the root weight.
1.2.2, field transplanting survival rate contrast test.
And (3) adopting a random block design, planting 100 seedlings in the field every time, and counting the survival rate after survival.
1.2.3 light substrate mesh bag seedling and contrast drought tolerance test of tissue culture seedlings
Adopting a comparison method, performing drought tolerance tests once every month in 3, 4, 5, 6 and 7 months, and counting and observing the drought tolerance of the temporary planted seedlings cultivated in the two modes. In the greenhouse, after the seedlings cultivated in the two modes are drenched with sufficient water, a drought test is started, and how long the seedlings wither is observed and counted.
1.2.4, investigation time
Investigating the survival rate of the temporary planting after the temporary planting of the tissue culture seedlings for 10 days; measuring the number of green leaves, the plant height and the root weight after 30d of temporary planting; temporarily planting for 30d, transplanting to a field for planting, and counting the survival rate after survival; drought tolerance tests were performed every month starting from 3 months.
1.2.5 statistical analysis of test data
The data were computationally processed using the new repolarization method using Excel2007 and DPS data processing systems.
2. Results and analysis
2.1 influence of light-weight matrix mesh bag seedling culture of tissue culture seedlings on survival rate of temporary planting
From the results in table 1, the survival rate of the osmanthus sugar 42 and 58 cultured by the mesh bag filled with the tissue culture seedling matrix is not obviously different from that of CK, while the survival rate of the osmanthus sugarcane 1 cultured by the mesh bag reaches a significant level.
TABLE 1 comparison of survival rates of mesh bag seedlings with tissue culture seedling matrix and control temporary planting
2.2 influence of light-weight substrate mesh bag seedling culture of tissue culture seedlings on the number of green leaves, plant height and root weight
From the analysis of the results in table 2, the green leaf numbers of 3 varieties are more than CK when the net bags filled with the tissue culture seedling substrates are used for raising seedlings, and all the varieties reach the significant difference level; the height of the mesh bag seedling raising plant filled with the tissue culture seedling matrix is higher than that of CK seedling raising by more than 2.2cm and reaches a significant difference level; from the root weight results, the root system of the seedlings of the mesh bag filled with the tissue culture seedling matrix is heavier than CK, and 3 varieties reach obvious difference level. The root is not damaged when the net bag containing the tissue culture seedling substrate is used for raising seedlings, the traditional plug tray is used for raising seedlings, part of root systems penetrate below the plug tray, and the roots penetrating below the plug tray are broken when the seedlings are raised, so that the root is damaged.
Table 2 comparison of the number of green leaves, plant height and root weight of the seedlings grown in the mesh bag with the matrix of the tissue culture seedlings
2.3 influence of light-weight matrix mesh bag seedling culture of tissue culture seedlings on field transplanting survival rate
The mesh bag matrix filled with the tissue culture seedling matrix is loose, has strong water retention and contains certain nutrients, and the tissue culture seedlings can quickly root in the mesh bag filled with the tissue culture seedling matrix. From the results of the test in table 3, the survival rate of the net bag seedlings filled with the tissue culture seedling matrix reaches 100%, and the ratio of 3 varieties to CK reaches a significant difference level.
TABLE 3 comparison of the survival rate of the net bag seedlings with the tissue culture seedling matrix in the control field
2.4 influence of mesh bag seedling culture with tissue culture seedling matrix on drought resistance of seedlings
The results are shown in Table 4. The light matrix mesh bag has strong water-retaining property and does not damage roots. In 3 and 4 months of low-temperature rainy days, seedlings cultivated by the mesh bags filled with the tissue culture seedling matrixes are in dry soil, the seedlings wilted after 12 days and 9 days, and the seedlings cultivated by the tray yellow soil wilted after 4 days and 2 days; the net bags with the tissue culture seedling matrixes planted in dry soil can be wilted after 4 days in 6 and 7 months with higher temperature and stronger sunlight, and the tray loess seedlings can be wilted only after 0.5 day. The test result shows that the drought resistance of the mesh bag seedling filled with the tissue culture seedling matrix is stronger, and the mesh bag seedling is suitable for large-area planting in machinery and manpower.
TABLE 4 comparison of the number of wilting days with the control for the mesh bag seedlings with the tissue culture substrate
| Type (B) | 3 month | 4 month | Month 5 | 6 month | 7 month |
| Non-woven fabric mesh bag light substrate | 12 days | 9 days | 5 days | 4 days | 4 days |
| Yellow soil | 4 days | 2 days | 1 day | 0.5 day | 0.5 day |
Test example 2 comparison of advantages and disadvantages of mesh bag seedling culture with tissue culture seedling substrate
The results are shown in Table 5.
TABLE 5 comparison table of advantages and disadvantages of mesh bag seedlings filled with tissue culture seedling matrix and comparison
From the comparison of table 5, the advantage of seedling raising with the light matrix mesh bag is greater than that of seedling raising with plug yellow soil, the seedling raising with the sugarcane tissue culture seedling can be popularized in a large area, the only disadvantage is that the seedling raising cost is slightly high, and the water management is enhanced in the stage of cutting roots and controlling seedlings of the seedlings.
The light matrix mesh bag seedling raising effect for the tissue culture seedlings of the sugarcane of the gramineous crops is consistent with the fast growing effect of woody trees, and the cultured tissue culture pseudo-planted seedlings of the sugarcane have the characteristics of developed root system, good air root repairing effect, more lateral roots, no seedling reviving period, long seedling storage time and the like. The bags are not needed to be removed during the field planting, the direct planting is realized, and the planting survival rate is high; the seedlings are light in weight, convenient to carry, time-saving and labor-saving in field planting, high in planting efficiency and low in production cost; the root system of the bred temporary planting seedlings is tightly interwoven with the matrix, the sizes of the seedlings are uniform, and the temporary planting seedlings are particularly suitable for mechanical planting. The light matrix is loose, breathable and permeable, the root system grows quickly, the seedling-leaving time is 20 days earlier than that of the traditional yellow soil seedling culture, the seedling-culturing time of the nursery is reduced, and the utilization rate of the nursery is improved. The light matrix mesh bag seedlings are planted in the field without a seedling reviving period, the root systems directly extend to the periphery and downwards after planting, the growth vigor of the seedlings is superior to that of yellow soil plug seedlings, the growth period of the seedlings is long, the growth vigor is vigorous, the tissue culture seedlings of the sugarcane can be widely popularized and planted, and the transformation that the tissue culture seedlings replace the traditional seed stems to plant the raw sugarcane is further realized.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A drought-tolerant sugarcane tissue culture seedling raising method suitable for mechanical planting is characterized by comprising the following steps:
s1, seed selection: selecting a variety with tillering force more than equal;
s2, single-plant temporary planting of sugarcane tissue culture rooted seedlings: transplanting the sugarcane tissue culture rooted seedlings into a seedling culture container for culture, hardening the seedlings and transplanting the seedlings into single seedlings with independent root systems by single plants after cluster planting and rooting;
s3, single-plant seedling temporary planting management: spraying a growth regulator on the single seedlings to control the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
s4, outplanting: outplanting when the seedling grows to 10-12 leaves, controlling water and fertilizer 5-7 days before outplanting, and spraying tillering promoting medicine.
2. The drought-tolerant mechanically-planted sugarcane tissue culture seedling raising method according to claim 1, wherein the variety comprises sugar cane and fruit cane.
3. The drought-enduring and mechanically-planted sugarcane tissue culture seedling raising method as claimed in claim 1, wherein the seedling raising container is a cylindrical mesh bag filled with a tissue culture seedling substrate, and the diameter of the mesh bag is 2-6cm, the height of the mesh bag is 5-12cm, and the tissue culture seedling substrate is added.
4. The drought-enduring and mechanically-planted sugarcane tissue culture seedling raising method as claimed in claim 3, wherein the tissue culture seedling substrate comprises 5-10 parts by weight of chaff, 12-15 parts by weight of straw, 2-5 parts by weight of plant ash and 3-7 parts by weight of waste bagasse.
5. The drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method according to claim 4, wherein the tissue culture seedling substrate comprises 7 parts by weight of chaff, 13 parts by weight of straw, 3 parts by weight of plant ash and 5 parts by weight of waste bagasse.
6. The method for raising seedlings of sugarcane tissue culture seedlings with drought tolerance and adaptability to mechanical planting as claimed in claim 1, wherein the growth regulator comprises 5-10ppm of indoleacetic acid, 100-120ppm of paclobutrazol and 0.5-1 wt% of monopotassium phosphate.
7. The drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method according to claim 6, wherein the spraying amount of the growth regulator is 14-17m per liter of the pesticide solution2And (5) nursery garden.
8. The drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method as claimed in claim 1, wherein the tillering-promoting drug comprises 150-200ppm compound sodium nitrophenolate and 2-4 wt% of urea.
9. The drought-tolerant and mechanically-planted sugarcane tissue culture seedling raising method according to claim 8, wherein the spraying amount of the tillering-promoting drug is 10-14m per liter of liquid medicine2And (5) nursery garden.
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|---|---|---|---|---|
| JP2004041052A (en) * | 2002-07-10 | 2004-02-12 | Hiroshima Pref Gov | Lightweight seedling medium for vegetable seedling and flowerbed seedling |
| CN104744107A (en) * | 2013-12-25 | 2015-07-01 | 上海宇强贸易有限公司 | Biological seedling growing matrix |
| CN104770173A (en) * | 2015-04-13 | 2015-07-15 | 云南省农业科学院甘蔗研究所 | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings |
| CN113508733A (en) * | 2021-08-13 | 2021-10-19 | 广西壮族自治区农业科学院 | Sugarcane tissue culture seedling cultivation method suitable for mechanical planting |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004041052A (en) * | 2002-07-10 | 2004-02-12 | Hiroshima Pref Gov | Lightweight seedling medium for vegetable seedling and flowerbed seedling |
| CN104744107A (en) * | 2013-12-25 | 2015-07-01 | 上海宇强贸易有限公司 | Biological seedling growing matrix |
| CN104770173A (en) * | 2015-04-13 | 2015-07-15 | 云南省农业科学院甘蔗研究所 | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings |
| CN113508733A (en) * | 2021-08-13 | 2021-10-19 | 广西壮族自治区农业科学院 | Sugarcane tissue culture seedling cultivation method suitable for mechanical planting |
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