CN113508733A - Sugarcane tissue culture seedling cultivation method suitable for mechanical planting - Google Patents
Sugarcane tissue culture seedling cultivation method suitable for mechanical planting Download PDFInfo
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- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 64
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 60
- 238000012364 cultivation method Methods 0.000 title claims description 13
- 239000003630 growth substance Substances 0.000 claims abstract description 48
- 238000005507 spraying Methods 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 241000196324 Embryophyta Species 0.000 claims abstract description 22
- 239000003337 fertilizer Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 21
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 21
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 20
- 239000005985 Paclobutrazol Substances 0.000 claims description 20
- DARPYRSDRJYGIF-PTNGSMBKSA-N (Z)-3-ethoxy-2-naphthalen-2-ylsulfonylprop-2-enenitrile Chemical compound C1=CC=CC2=CC(S(=O)(=O)C(\C#N)=C/OCC)=CC=C21 DARPYRSDRJYGIF-PTNGSMBKSA-N 0.000 claims description 19
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 19
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 19
- 239000002689 soil Substances 0.000 claims description 13
- 241000238631 Hexapoda Species 0.000 claims description 10
- 201000010099 disease Diseases 0.000 claims description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000007796 conventional method Methods 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 239000005886 Chlorantraniliprole Substances 0.000 claims description 6
- 239000005760 Difenoconazole Substances 0.000 claims description 6
- PSOVNZZNOMJUBI-UHFFFAOYSA-N chlorantraniliprole Chemical compound CNC(=O)C1=CC(Cl)=CC(C)=C1NC(=O)C1=CC(Br)=NN1C1=NC=CC=C1Cl PSOVNZZNOMJUBI-UHFFFAOYSA-N 0.000 claims description 6
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 claims description 6
- 239000000375 suspending agent Substances 0.000 claims description 6
- 241000209504 Poaceae Species 0.000 claims description 5
- 230000002265 prevention Effects 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 4
- 238000009966 trimming Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000012136 culture method Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 15
- 238000012360 testing method Methods 0.000 description 12
- 235000019082 Osmanthus Nutrition 0.000 description 8
- 241000333181 Osmanthus Species 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- PWVXXGRKLHYWKM-UHFFFAOYSA-N 5-[2-(benzenesulfonyl)ethyl]-3-[(1-methylpyrrolidin-2-yl)methyl]-1h-indole Chemical compound CN1CCCC1CC(C1=C2)=CNC1=CC=C2CCS(=O)(=O)C1=CC=CC=C1 PWVXXGRKLHYWKM-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 239000005984 Mepiquat Substances 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- NNCAWEWCFVZOGF-UHFFFAOYSA-N mepiquat Chemical compound C[N+]1(C)CCCCC1 NNCAWEWCFVZOGF-UHFFFAOYSA-N 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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Abstract
The invention belongs to the technical field of seedling culture, and particularly relates to a sugarcane tissue culture seedling culture method suitable for mechanical planting, which comprises the following steps: (one) seed selection: selecting sugarcane varieties to perform tissue culture on rooted seedlings; secondly, single-plant temporary planting of the sugarcane tissue culture rooted seedlings; (III) single plant temporary planting management: when the seedling ages to 4-5 leaves and 7-8 leaves, respectively spraying a growth regulator A and a growth regulator B; (IV) outplanting: outplanting when the seedlings grow to 10-12 leaves, and controlling water and fertilizer 5-7 days before outplanting; cutting off 2/3 leaves one day before outplanting, outplanting on day 2, and transplanting to field. The method effectively shortens the internode length of the underground part of the sugarcane plant, increases the number of the internodes, the thickness of the sugarcane nodes and the number of the roots, promotes the sugarcane to form a developed root system, and achieves the effects of increasing the yield and preventing the sugarcane from lodging; the mechanical planting reduces the production cost and improves the sugarcane planting benefit.
Description
Technical Field
The invention belongs to the technical field of seedling culture, and particularly relates to a sugarcane tissue culture seedling culture method suitable for mechanical planting.
Background
Sugar cane is a temperate and tropical crop, a raw material for sucrose production, and ethanol can be extracted as an energy substitute. The sugarcane contains abundant sugar and water, also contains various vitamins, fat, protein, organic acid, calcium, iron and other substances which are very beneficial to the metabolism of a human body, and is mainly used for preparing sugar.
The root system of the sugarcane is mainly distributed in a soil layer of 0-40cm, when the sugarcane grows 2-3 true leaves, the root source of the underground node at the base part of the seedling germinates plant roots, and the plant roots of the sugarcane plants gradually increase to the tillering initial stage, tillering full stage and the elongation stage of the joints, particularly, the thick elongation of the stems of the sugarcane occurs in the elongation stage, so that the absorption strength of the sugarcane to rich water is high, and the plants are required to have strong absorption root systems. If the seedlings are weak and small in the seedling stage of the sugarcane, the stems of sugarcane plants are small and have few root points and few nodes buried in soil, so that the root systems are not developed.
The sugarcane tissue culture seedling is mainly used for breeding seed stems, and if the tissue culture seedling is used for producing raw sugarcane, underground sections of the sugarcane are few and thin. Because the number of underground sugarcane nodes is small, a strong root group foundation is not formed, and the base stem is small, the lodging rate is high. In addition, sugarcane production is labor-intensive industry, and the manual planting cost is high. Based on the defects, a more optimized sugarcane tissue culture seedling cultivation method suitable for mechanical planting needs to be provided.
Disclosure of Invention
Aiming at the problems, a sugarcane tissue culture seedling cultivation method suitable for mechanical planting is needed to be provided, the internode length of the underground part of a sugarcane plant can be effectively shortened, the node number is increased, the node thickness and the rooting number are increased, the sugarcane is promoted to form a developed root system, and the effects of increasing the yield and preventing the sugarcane from lodging are achieved; the mechanical planting reduces the production cost and improves the sugarcane planting benefit.
In order to achieve the purpose, the invention adopts the following technical scheme: a cultivation method of sugarcane tissue culture seedlings suitable for mechanical planting comprises the following steps:
(one) seed selection: selecting sugarcane varieties to perform tissue culture on rooted seedlings;
secondly, single-plant temporary planting of the sugarcane tissue culture rooted seedlings: after the tissue culture rooting seedlings of the sugarcane are temporarily planted in a cluster mode or the tissue culture seedlings take roots individually, hardening and dividing the seedlings into individual seedlings with independent root systems, and temporarily planting the individual seedlings in a seedling culture container filled with a seedling culture substrate in 9-12 months of each year for culture;
(III) single plant temporary planting management: when the seedling ages to 4-5 leaves, spraying growth regulator A on the single plant seedling by leaf surface spraying, wherein each liter of liquid medicine is sprayed by 10-12m2Nursery garden; when the seedling ages to 7-8 leaves, the growth regulator B is sprayed on the single plant seedling again in the same way of spraying the leaf surface, and 10-12m of pesticide liquid is sprayed every liter2Nursery garden; controlling the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
(IV) outplanting: outplanting when the seedlings grow to 10-12 leaves, and controlling water and fertilizer 5-7 days before outplanting; cutting off 2/3 leaves one day before outplanting, outplanting on day 2, and transplanting to field.
Preferably, in the step (two), the seedling substrate is sandy soil, mellow soil or yellow soil.
Preferably, in the step (two), the seedling raising container is a seedling raising plug or a seedling raising bag with the caliber of 4-6cm and the depth of 4-10 cm.
Preferably, in the step (III), the raw materials of the growth regulator A comprise 50-100ppm of paclobutrazol, 200ppm of mepiquat chloride and 0.2-0.3% of monopotassium phosphate.
Preferably, in the step (III), the raw materials of the growth regulator B comprise 100ppm of paclobutrazol, 400ppm of mepiquat chloride and 200ppm of monopotassium phosphate, and the concentration of the raw materials is 0.3-0.4%.
Preferably, in the step (four), after the leaves of the individual seedlings are trimmed, 10 ml of 20% chlorantraniliprole suspending agent is taken and added with 30 kg of water, and the amount of the suspending agent is 10 to 15m per liter2Spraying in nursery garden, adding 800-1200 times of 10% difenoconazole into water per liter, and adding 10-15m2The insecticide is sprayed in a nursery garden and is used for preventing insects and diseases, and the percentages of the 20% chlorantraniliprole and the 10% difenoconazole refer to weight percentage.
Compared with the prior art, the invention has the beneficial effects that:
1. the growth regulator A and the growth regulator B of the method take paclobutrazol, mepiquat chloride and monopotassium phosphate as components, when the seedling age is 4-5 leaves and 7-8 leaves, the two different periods are sprayed on the leaf surfaces, the elongation growth of the lower internodes of the sugarcane is inhibited, the internode length is shortened, the transverse growth of the internodes is promoted, and the thickness of the sugarcane stems is increased, so that the underground internodes and the thickness of the sugarcane internodes of the sugarcane can be increased under the condition of the same soil depth, the developed root system of the sugarcane is promoted, the water and fertilizer absorption range of the sugarcane is greatly improved, and the effects of increasing the yield and preventing and controlling lodging of the sugarcane are achieved.
2. The single-plant seedling cultured by the method has 10-13 sections, thick leaves and high planting survival rate, is suitable for mechanical planting, has high planting efficiency, reduces the cost and labor cost, and meets the requirements of industrialized and mechanized planting of seedling production.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to these examples. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The test site is located in a test field of Guangxi Zhuang autonomous region agro-scientific college, the cultivated variety is Gui sugar No. 42, and the cultivation method of the sugarcane tissue culture seedling suitable for mechanical planting comprises the following steps:
(one) seed selection: selecting osmanthus sugar No. 42 tissue culture rooted seedlings;
secondly, single-plant temporary planting of the sugarcane tissue culture rooted seedlings: after the tissue culture rooting seedlings of the sugarcane are temporarily planted in a cluster mode or the tissue culture seedlings take roots individually, hardening and dividing the seedlings into individual seedlings with independent root systems, and temporarily planting the individual seedlings in a seedling raising container filled with sandy soil in 09 months in 2019 for cultivation; the seedling raising container is a seedling raising plug tray with the caliber of 4cm and the depth of 4 cm;
(III) single plant temporary planting management: when the seedling ages to 4 leaves, spraying growth regulator A on the single plant seedling by leaf surface spraying, and spraying 10m of liquid medicine per liter2Nursery, growth regulator A comprises paclobutrazol 50ppm, mepiquat chloride 100ppm and monopotassium phosphate 0.2%; when the seedling ages to 7 leaves, the growth regulator B is sprayed on the single plant seedling again in a leaf surface spraying mode, and 10m of liquid medicine is sprayed per liter2Nursery, growth regulator B comprises paclobutrazol 100ppm, mepiquat chloride 200ppm and monopotassium phosphate 0.3%; controlling the height of the single seedlings to be 20cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
(IV) outplanting: when the seedlings grow to 10 leaves, outplanting, and controlling water and fertilizer 5 days before outplanting; cutting off 2/3 leaf blade one day before outplanting, adding 10 ml 20% chlorantraniliprole suspending agent, adding 30 kg water, and adding 10m per liter2Spraying in nursery garden, adding 10% difenoconazole with 800 times of water per liter to 10m2Spraying the fertilizer in nursery garden for preventing insects and diseases, and transplanting the fertilizer in field on day 2。
Example 2
The test site is located in a test field of Guangxi Zhuang autonomous region agro-scientific college, the cultivated variety is Gui sugar No. 42, and the cultivation method of the sugarcane tissue culture seedling suitable for mechanical planting comprises the following steps:
(one) seed selection: selecting osmanthus sugar No. 42 tissue culture rooted seedlings;
secondly, single-plant temporary planting of the sugarcane tissue culture rooted seedlings: after the tissue culture rooting seedlings of the sugarcane are temporarily planted in a cluster mode or the tissue culture seedlings take roots individually, hardening and dividing the seedlings into individual seedlings with independent root systems, and temporarily planting the individual seedlings in a seedling raising container filled with mellow soil in 2019 and 10 months for cultivation; the seedling container is a seedling bag with the caliber of 4.5cm and the depth of 7.0 cm;
(III) single plant temporary planting management: when the seedling ages to 4 leaves, spraying growth regulator A on the single plant seedling by leaf surface spraying, and spraying 11m of liquid medicine per liter2Nursery, growth regulator A comprises paclobutrazol 80ppm, mepiquat chloride 150ppm and monopotassium phosphate 0.2%; when the seedling ages to 7 leaves, the growth regulator B is sprayed on the single plant seedling again in a leaf surface spraying mode, and 11m of liquid medicine is sprayed per liter2Nursery, growth regulator B comprises paclobutrazol 200ppm, mepiquat chloride 300ppm and monopotassium phosphate 0.3%; controlling the height of the single seedlings to be 25cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
(IV) outplanting: the seedlings are outplanted when the seedlings grow to 11 leaves, and water and fertilizer are controlled 5 days before outplanting; cutting off 2/3 leaf blade one day before outplanting, adding 10 ml 20% chlorantraniliprole suspending agent, adding 30 kg water, and adding per liter to 13m2Spraying in nursery garden, adding 10% difenoconazole 1000 times water, and adding 13m per liter2Spraying the fertilizer in a nursery garden for preventing insects and diseases, and transplanting the fertilizer out of the nursery garden to a field on day 2.
Example 3
The test site is located in a test field of Guangxi Zhuang autonomous region agro-scientific college, the cultivated variety is Gui sugar No. 42, and the cultivation method of the sugarcane tissue culture seedling suitable for mechanical planting comprises the following steps:
(one) seed selection: selecting osmanthus sugar No. 42 tissue culture rooted seedlings;
secondly, single-plant temporary planting of the sugarcane tissue culture rooted seedlings: after the tissue culture rooting seedlings of the sugarcane are temporarily planted in a cluster mode or the tissue culture seedlings take roots individually, hardening and dividing the seedlings into individual seedlings with independent root systems, and temporarily planting the individual seedlings in a seedling raising container filled with yellow soil in 2019 and 12 months for cultivation; the seedling raising container is a seedling raising bag with the caliber of 6cm and the depth of 10 cm;
(III) single plant temporary planting management: when the seedling ages to 5 leaves, spraying growth regulator A on the single plant seedling by leaf surface spraying, and spraying 12m of liquid medicine per liter2Nursery, growth regulator A comprises paclobutrazol 100ppm, mepiquat chloride 200ppm and monopotassium phosphate 0.3%; when the seedling ages to 8 leaves, the growth regulator B is sprayed on the single plant seedling again in the same way of spraying the leaf surface, and the spraying amount is 12m per liter of the pesticide solution2Nursery, growth regulator B comprises paclobutrazol 250ppm, mepiquat chloride 400ppm and monopotassium phosphate 0.4%; controlling the height of the single seedlings to be 30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
(IV) outplanting: when the seedlings grow to 12 leaves, the seedlings are outplanted, and water and fertilizer are controlled 5 days before outplanting; cutting off 2/3 leaf blade one day before outplanting, adding 10 ml 20% chlorantraniliprole suspending agent, adding 30 kg water, and adding per liter to 15m2Spraying in nursery garden, adding 10% difenoconazole 1200 times of water, and adding 15m per liter2Spraying the fertilizer in a nursery garden for preventing insects and diseases, and transplanting the fertilizer out of the nursery garden to a field on day 2.
Comparative example 1
The difference from example 2 is: in the step (III), the growth regulator A comprises 40ppm of paclobutrazol, 80ppm of mepiquat chloride and 0.1% of monopotassium phosphate; the growth regulator B comprises paclobutrazol 80ppm, mepiquat chloride 180ppm and monopotassium phosphate 0.2%, and the rest steps and parameters are the same as those of example 2.
Comparative example 2
The difference from example 2 is: in the step (III), the growth regulator A comprises 120ppm of paclobutrazol, 220ppm of mepiquat chloride and 0.4% of monopotassium phosphate; growth regulator B paclobutrazol 280ppm, mepiquat chloride 420ppm and potassium dihydrogen phosphate 0.5%, the rest steps and parameters are the same as example 2.
Comparative example 3
The difference from example 2 is: in the step (III), the growth regulator A is paclobutrazol 80 ppm; growth regulator B was mepiquat chloride 300ppm and the remaining steps and parameters were the same as in example 2.
Comparative example 4
The difference from example 2 is: in the step (III), the growth regulator A comprises 80ppm of paclobutrazol and 0.2 percent of monopotassium phosphate; growth regulator B comprised mepiquat chloride 300ppm and potassium dihydrogen phosphate 0.3%, the remaining steps and parameters were the same as in example 2.
Comparative example 5
The difference from example 2 is: in the step (III), the growth regulator A comprises 80ppm of chlormequat chloride, 150ppm of mepiquat chloride and 0.2% of monopotassium phosphate; the growth regulator B comprises 200ppm of paclobutrazol, 300ppm of chlormequat chloride and 0.3% of monopotassium phosphate, and the rest steps and parameters are the same as those of example 2.
Test example 1: effect of the different component contents of the growth regulator on the invention
Taking 6 test fields with the planting area of 1 mu, wherein 5 test fields respectively correspond to examples 1-3 and comparative examples 1-2, the verification of planting of the osmanthus sugar No. 42 is carried out, the verification of planting of the osmanthus sugar No. 42 in the other test field is carried out according to a conventional method, and the specific results are shown in table 1.
TABLE 1 comparison of effects of osmanthus sugar No. 42 planted in the present invention in examples 1-3 and comparative examples 1-2 and conventional method
As can be seen from table 1, it is,
(1) examples 1-3 are the component contents selected for growth regulator A and growth regulator B of the method of the invention, data in the table show that the method of the invention can effectively reduce the internode length of the sugarcane below the soil surface, further increase the node number, increase the thickness of the sugarcane node and the number of roots, promote the root system to be developed, reduce the lodging rate, improve the effective stem number and increase the yield per mu;
(2) the contents of the components of the growth regulator A and the growth regulator B in the comparative example 1 are both less than those in the example 2 of the present application, and the contents of the components of the growth regulator A and the growth regulator B in the comparative example 2 are both higher than those in the example 2 of the present application, and as can be seen from the data in the table, although the number of underground nodes, the diameter of stems, the height of plants and the like in the comparative examples 1-2 are superior to those in the conventional method, compared with the method of the present application, the number of underground nodes, the diameter of stems, the height of plants and the like are inferior to those of sugarcane planted by the method of the present application, and the technical effect of the method of the present application is superior.
Test example 2: effect of different Components of growth regulators on the invention
2 test fields with the planting area of 1 mu are taken, and verification is respectively carried out corresponding to the planting of the osmanthus sugar No. 42 in the comparative examples 3 and 4, and the specific results are shown in the table 2.
Table 2 comparison of the effect of the present invention using comparative examples 3-4 and the osmanthus sugar 42 planted in example 2
As can be seen from table 2, it is,
the components of the growth regulator A and the growth regulator B in the comparative example 3 are in a single category, the components of the growth regulator A and the growth regulator B in the comparative example 4 are in one category less than that of the invention in the application, the data in the table show that the parameters of underground node number, stem diameter, high plant height and the like in the comparative example 3-5 are less than those of the method in the application, and the combination of the table 1 and the table 2 shows that when the raw material composition content of the growth regulator A is 50-100ppm of paclobutrazol, 200ppm of mepiquat chloride and 0.2-0.3% of monopotassium phosphate, and the raw material composition content of the growth regulator B is 250ppm of paclobutrazol 100, 400ppm of mepiquat and 0.3-0.4% of monopotassium phosphate, the inhibition effect on the underground node number of sugarcane is particularly obvious, and the parameters such as stem diameter, plant height, acre yield and the like are all excellent.
It will be appreciated by persons skilled in the art that the above discussion of any embodiment is merely exemplary and is not intended to imply that the scope of the disclosure, including the claims, is limited to these examples, that features from any of the above embodiments, or from different embodiments, may be combined, that the steps of performing may be performed in any order, and that there are numerous other variations of the various aspects of the invention described above that are not provided in detail for the sake of brevity. Therefore, any omissions, modifications, substitutions, improvements and the like that may be made without departing from the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (6)
1. A cultivation method of sugarcane tissue culture seedlings suitable for mechanical planting is characterized by comprising the following steps:
(one) seed selection: selecting sugarcane varieties to perform tissue culture on rooted seedlings;
secondly, single-plant temporary planting of the sugarcane tissue culture rooted seedlings: after the tissue culture rooting seedlings of the sugarcane are temporarily planted in a cluster mode or the tissue culture seedlings take roots individually, hardening and dividing the seedlings into individual seedlings with independent root systems, and temporarily planting the individual seedlings in a seedling culture container filled with a seedling culture substrate in 9-12 months of each year for culture;
(III) single plant temporary planting management: when the seedling ages to 4-5 leaves, spraying a growth regulator A to the single-plant seedlings in a leaf surface spraying mode, and spraying liquid medicine per liter of a nursery garden with square meters of 10-12; when the seedling ages to 7-8 leaves, spraying the growth regulator B to the single-plant seedlings in a leaf surface spraying mode again, and spraying 10-12 square meters per liter of liquid medicine to the nursery; controlling the height of the single seedlings to be 20-30cm before outplanting; the management of the water fertilizer, the prevention and treatment methods of diseases, insects and grasses is carried out according to the conventional method;
(IV) outplanting: outplanting when the seedlings grow to 10-12 leaves, and controlling water and fertilizer 5-7 days before outplanting; cutting off 2/3 leaves one day before outplanting, outplanting on day 2, and transplanting to field.
2. A method for cultivating tissue culture seedlings of sugar cane adapted for mechanical planting according to claim 1, wherein in the second step, the seedling substrate is sandy soil, mellow soil or yellow soil.
3. A sugarcane tissue culture seedling cultivation method suitable for mechanical planting as claimed in claim 1, wherein in the step (two), the seedling container is a seedling hole tray or a seedling bag with a caliber of 4-6cm and a depth of 4-10 cm.
4. The cultivation method of tissue culture seedlings of sugarcane adapted for mechanical planting as claimed in claim 1, wherein in step (III), the raw materials of the growth regulator A comprise paclobutrazol 50-100ppm, mepiquat chloride 100-200ppm and monopotassium phosphate 0.2% -0.3%.
5. The cultivation method of tissue culture seedlings of sugarcane suitable for mechanical planting as claimed in claim 1, wherein in step (III), the raw materials of the growth regulator B comprise paclobutrazol 100-250ppm, mepiquat chloride 200-400ppm and monopotassium phosphate 0.3% -0.4%.
6. The tissue culture seedling cultivation method for sugarcane suitable for mechanical planting as claimed in claim 1, wherein in step (four), after trimming leaves of a single plant, 10 ml of 20% chlorantraniliprole suspending agent is taken, after 30 kg of water is added, spraying is carried out on a nursery garden of 10-15 square meters per liter, then 1200 times of water is added to 10% difenoconazole, and spraying is carried out on the nursery garden of 10-15 square meters per liter, so as to prevent insects and diseases.
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