CN103314767B - Schima superba cold-resistant strain tissue culture seedling twig short ear cutting propagation method - Google Patents
Schima superba cold-resistant strain tissue culture seedling twig short ear cutting propagation method Download PDFInfo
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- CN103314767B CN103314767B CN201310293371.9A CN201310293371A CN103314767B CN 103314767 B CN103314767 B CN 103314767B CN 201310293371 A CN201310293371 A CN 201310293371A CN 103314767 B CN103314767 B CN 103314767B
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Abstract
The invention discloses a schima superba cold-resistant strain tissue culture seedling twig short ear cutting propagation method. The method is characterized by including the following steps that 1) cutting media and root growth promoting agents are prepared; 2) when a schima superba cold-resistant strain tissue culture seedling twig grows to be 50-60cm high through breeding, a branch with the length of 20-30cm is cut into small sections with the length of 3-5cm, and all leaves are reserved, soaked in the prepared root growth promoting agents by 2-5s and then inserted into the prepared media; 3) cutting ears are cut in a hole plate with the media, then under the condition full of light and fog, the relative humidity is kept to be 85%-95%, the temperature is kept to be 20-30 DEG C, roots of the cutting ears begin to produce calluses within 12-15 days, root systems grow from the calluses with 20-25 days spent, new buds grow 30 days later, and transplanting is carried out after 45 days. Twig short ears are clipped from tissue culture transplanting survival seedlings to be cut, not only are good characters of original schima superba stock plants kept, but also the cutting survival rate exceeds 92%, operation is simple, cost is low, and efficiency is high.
Description
Technical field
The present invention relates to the cuttage breeding method of a Plants, specifically, relate to a kind of Schima superba and to resist cold strain group training transplant survival seedling spray single node cutting propagation method, belong to achievements in forest-tree seedling vegetative propagation technique field.
Background technology
Schima superba (Schima superba) belongs to Theaceae Schima, is the evergreen megaphanerophyte of subtropics zonality, is distributed widely in 12 provinces and regions, south as precious high quality timber and fast-growing view fire prevention characteristic seeds.1. Schima superba trunk end is straight, and timber heavily fortified point is heavy fine and close, even structure, and mechanical property is good, is the high-quality wooden artistic commerical tree species such as timber floor, wood furniture, wooden toy, Wood craft; 2. Schima superba growth rapidly, and few damage by disease and insect, requires not tight to soil condition, Drought hardiness is strong, tree crown is dense simultaneously, and blade heavy leather matter, water content are large, few containing grease, burning-point is higher, rudiment power strong, is the main seeds of southern Forest Belts of Biological Fire Prevention structure and excellent high resistance ecoscape Shelter-wood; 3. easy, the early stage fast-growing of Schima superba cultivation, widely southern each province payes attention to and likes, annual sowing quantity all reaches tens thousand of kilograms, is the first precious high-quality broad-leaved material reproducting tree species in subtropical zone.
Schima superba mainly seed propagation is main, and grafting and cottage propagation are studied less.In the prior art, cottage propagation mainly utilizes the spray of Schima superba to carry out cuttage root-taking,
As in " Schima superba Cutting test " literary composition that " Fujian Forestry science and technology " the 23rd volume the 3rd phase (1996) delivers, disclose a kind of cuttage breeding method of Schima superba, preferably Cutting conditions adopts concentration be the 300ppm gibberellin processing time to be 3 hours, cutting length is 10cm, time is September, and cutting average survival can reach more than 81%.In " Schima superba full exposure epicormic branch cuttage raising technology " literary composition that and for example " modern agriculture science and technology " the 14th phase (2011) delivers, disclose a kind of Schima superba full exposure cuttage and seedling culture propagation method, the spray that clip gives birth to semi-lignified then makes cuttings, select 10 years raw age of trees to be scion-seed tree, choose and grow the healthy and strong semi-lignified young sprout without damage by disease and insect of normal growth as cuttings.Spike length about 10cm, suitable for reading is plane, and end opening is horse ear shape, 2 ~ 3 leaves are stayed in upper end, can cut off leaf 1/2 part, and cuttings base portion 300ppm gibberellin is soaked 3h, by every for cuttings 50 1 bundles, spray with carbendazim or tpn 800 times of liquid or dip cuttings base portion, cutting plantation reaches 83%.
Report is yet there are no for utilizing the Schima superba strain group training transplant survival seedling spray single node cutting propagation method that resists cold.
Summary of the invention
Because cold-resistant Schima superba Schima superba strain tissue cultivating and seedling cost is relatively high, technical problem to be solved by this invention is to provide the spray single node cutting propagation method of a kind of easy and simple to handle, low cost, high-servival rate, efficient cold-resistant Schima superba plantlet in vitro fast.
For solving the problems of the technologies described above, the invention provides Schima superba and to resist cold strain plantlet in vitro spray single node cutting propagation method, it is characterized in that, comprise the following steps:
1) prepare cutting medium and rooting promoter: by perlite and peat by volume 5: 3 or 1: 1 ratio be hybridly prepared into cutting medium, with for subsequent use after 3% ferrous sulfate or 0.1% potassium permanganate solution spray disinfectant; Adopt indolebutyric acid IBA50 ~ 150mg and methyl α-naphthyl acetate NAA50 ~ 150mg mixed in equal amounts, the ethanol with a small amount of 95% mixes with thiamine hydrochloride 5 ~ 8mg after dissolving, and adds water and is settled to 1L, be made into rooting promoter solution, for subsequent use;
2) when the annual 6-10 month, Schima superba resists cold strain plantlet in vitro when growing to 50 ~ 60cm height, the long 20 ~ 30cm branch of clip, branch is cut into again the segment of 3 ~ 5cm, each short fringe cutting retains whole blade, after dipping 2 ~ 5s, inserts in the matrix of preparation in the rooting promoter prepared;
3) by cuttings cuttage containing matrix cave dish in, then under full light fog condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 12 ~ 15 days, cuttings base portion starts to produce callus, within 20 ~ 25 days, goes out root system from callus director, within 30 days, grows sprouting, within 45 days, transplant, survival rate more than 92% later.
The beneficial effect that the present invention reaches: the present invention adopts tissue cultures transplant survival seedling, by the short fringe of its tender stem segments of continuous clip as cutting, cuttage is carried out after plant growth regulator, vitamin mixed solution are as rooting promoter process, the merit of former plant can be kept, effectively can solve tissue cultures cost high problem simultaneously, method survival rate of the present invention is high, and cost is low, and efficiency is high.This method can cultivate a large amount of high quality seedling in a short time, improves Schima superba and to resist cold the promotion rate of excellent strain and scale.
Embodiment
Embodiment 1
1) prepare cutting medium and rooting promoter: by perlite and peat by volume 5: 3 ratio be hybridly prepared into cutting medium, with for subsequent use after 3% ferrous sulfate or 0.1% potassium permanganate solution spray disinfectant; Adopt indolebutyric acid IBA50mg and methyl α-naphthyl acetate NAA150mg mixed in equal amounts, the ethanol with a small amount of 95% mixes with thiamine hydrochloride 5mg after dissolving, and adds water and is settled to 1L, be made into rooting promoter solution, for subsequent use;
2) when the annual 6-10 month, Schima superba resists cold strain plantlet in vitro when growing to 50cm height, the long 20cm branch of clip, branch is cut into again the segment of 3cm, each short fringe cutting retains whole blade, after dipping 5s, inserts in the matrix of preparation in the rooting promoter prepared;
3) by cuttings cuttage containing matrix cave dish in, then under full light fog condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 15 days, cuttings base portion starts to produce callus, within 25 days, go out root system from callus director, within 30 days, grow sprouting, nutrient solution is used to spray cuttage seedling, growth promoting effects, described nutrient solution consists of: each amounts of components during preparation 1L solution is: ammonium nitrate 0.5g, potassium nitrate 0.6g, calcium chloride 0.18g, magnesium sulfate 0.24g, potassium dihydrogen phosphate 0.8g.Within 50 days, transplant, survival rate 92.5% later.
Embodiment 2
1) prepare cutting medium and rooting promoter: by perlite and peat by volume 1: 1 ratio be hybridly prepared into cutting medium, with for subsequent use after 3% ferrous sulfate or 0.1% potassium permanganate solution spray disinfectant; Adopt indolebutyric acid IBA150mg and methyl α-naphthyl acetate NAA50mg mixed in equal amounts, the ethanol with a small amount of 95% mixes with thiamine hydrochloride 8mg after dissolving, and adds water and is settled to 1L, be made into rooting promoter solution, for subsequent use;
2) when the annual 6-10 month, Schima superba resists cold strain plantlet in vitro when growing to 60cm height, the long 30cm branch of clip, branch is cut into again the segment of 5cm, each short fringe cutting retains whole blade, after dipping 5s, inserts in the matrix of preparation in the rooting promoter prepared;
3) by cuttings cuttage containing matrix cave dish in, then under full light fog condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 12 days, cuttings base portion starts to produce callus, within 20 days, go out root system from callus director, within 30 days, grow sprouting, nutrient solution is used to spray cuttage seedling, growth promoting effects, described nutrient solution consists of: each amounts of components during preparation 1L solution is: ammonium nitrate 0.8g, potassium nitrate 0.9g, calcium chloride 0.38g, magnesium sulfate 0.54g, potassium dihydrogen phosphate 1.0g.Within 45 days, transplant, survival rate 95.3% later.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (2)
1. Schima superba resists cold strain plantlet in vitro spray single node cutting propagation method, it is characterized in that: comprise the following steps:
1) prepare cutting medium and rooting promoter: by perlite and peat by volume 5: 3 or 1: 1 ratio be hybridly prepared into cutting medium, with for subsequent use after 3% ferrous sulfate or 0.1% potassium permanganate solution spray disinfectant; Adopt indolebutyric acid IBA50 ~ 150mg and methyl α-naphthyl acetate NAA50 ~ 150mg mixed in equal amounts, the ethanol with a small amount of 95% mixes with thiamine hydrochloride 5 ~ 8mg after dissolving, and adds water and is settled to 1L, be made into rooting promoter solution, for subsequent use;
2) when the annual 6-10 month, Schima superba resists cold strain group training transplant survival seedling when growing to 50 ~ 60cm height, long 20 ~ 30cm the branch of clip, branch is cut into again the segment of 3 ~ 5cm, each short fringe cutting retains whole blade, dip 2 ~ 5s in the rooting promoter prepared after, insert in the matrix of preparation;
3) by cuttings cuttage containing matrix cave dish in, then under full light fog condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 12 ~ 15 days, cuttings base portion starts to produce callus, within 20 ~ 25 days, go out root system from callus director, within 30 days, grow sprouting, within 45 days, transplant later.
2. Schima superba according to claim 1 resists cold strain plantlet in vitro spray single node cutting propagation method, it is characterized in that: in described step 3) in, after growing sprouting, nutrient solution is used to spray cuttage seedling, growth promoting effects, described nutrient solution consists of: each amounts of components during preparation 1L solution is: ammonium nitrate 0.5-0.8g, potassium nitrate 0.6-0.9g, calcium chloride 0.18-0.38g, magnesium sulfate 0.24-0.54g, potassium dihydrogen phosphate 0.8-1.0g.
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| CN105766300A (en) * | 2016-03-11 | 2016-07-20 | 浙江农林大学 | Method for raising ardisia violacea short sprouts by cutting |
| CN105918120B (en) * | 2016-04-26 | 2018-03-13 | 江苏碧云天农林科技有限公司 | A kind of mating system of Schima superba fast-growing new cold-resistant species |
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| CN102511279B (en) * | 2011-12-17 | 2013-10-16 | 湖南科技大学 | Method for promoting growth of evergreen arbors in manganese ore degraded area |
| CN102613089B (en) * | 2012-04-25 | 2013-12-18 | 江苏省林业科学研究院 | High-efficient in-vitro propagation method of 20-year-old schima superba big tree |
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