CN106165635B - A kind of llex cornuta lindl rapid propagation method - Google Patents

A kind of llex cornuta lindl rapid propagation method Download PDF

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Publication number
CN106165635B
CN106165635B CN201610631546.6A CN201610631546A CN106165635B CN 106165635 B CN106165635 B CN 106165635B CN 201610631546 A CN201610631546 A CN 201610631546A CN 106165635 B CN106165635 B CN 106165635B
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cuttings
cuttage
cornuta lindl
llex cornuta
soil
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CN106165635A (en
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马骧
邵美林
孙伟忠
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Jintan Changzhou Forestry Technical Guidance Station
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Jintan Changzhou Forestry Technical Guidance Station
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of llex cornuta lindl rapid propagation methods, belong to the asexual reproduction method of plant, and the method is the following steps are included: (1) maternal cultivation;(2) cuttage;(3) it is managed after inserting.This method is using garden to build the technical measures such as cutting bed by the cultivation of llex cornuta lindl cuttage elite stand, and in autumn using the cuttage of the lignifying branch of llex cornuta lindl, survival rate improves 20%~30% compared with spring, summer cuttage;Rooting percent is up to 93.8% or more, growth of cuttage seedlings is rapid, adaptable, and seedling is fast, the defects of spring and summer cuttage cuttings susceptible gene is high, survival rate is low, seedling cost is high is compensated for, there is important application value in the practice of llex cornuta lindl clone seedling breeding.

Description

A kind of llex cornuta lindl rapid propagation method
Technical field
The present invention relates to a kind of xylophyta asexual reproduction methods, are specifically related to llex cornuta lindl cuttage breeding method.
Background technique
Llex cornuta lindl (Ilex cornutaVar. fortunei), the natural variation product of Aquifoliaceae Ilex Chinese holly system Kind, blade full edge, evergreen shrubs or dungarunga.Its four seasons is evergreen, tree-like beauty, fruit it is red it is gorgeous do not fallen through the winter, be the Yangtze river basin Important ornamental tree species in afforestation.
The vegetative propagation of plant can keep maternal good characteristic, and the asexual reproduction method of llex cornuta lindl mainly uses skewer The methods of insert.In relevant report, based on spring and summer cuttage, spring cuttage increases llex cornuta lindl with temperature, and cuttage seeding is held Easy infection germ, cuttage survival rate is not high, and the cuttage seeding taken root will cause nursery stock it is still necessary to be transplanted again again in migration process Damage, and increase seedling cost;Summer shoot cutting honest summer high temperature period needs to have full exposure spraying system dress Setting can implement, and the method significantly limits the extensive breeding of seeling industry enterprise and nursery peasant household.
Summary of the invention
Goal of the invention: in order to overcome the problems, such as that existing Technique on Cuttage Propagation exists, further increase llex cornuta lindl cuttage at Motility rate reduces cuttings susceptible gene, expands the breeding coefficient of nursery stock, reduces seedling cost, discloses to present system stingless Chinese holly The efficient cuttage and seedling culture method of bone.
Technical solution: to achieve the above object, a kind of llex cornuta lindl rapid propagation method of the invention, it is characterized in that it by The following steps composition:
(1) maternal to cultivate
1. female parent selection: selecting 3~5 years raw no disease and pests harms, the llex cornuta lindl of robust growth is cuttings female parent;
2. soil application: winter 3000~5000kg of application of organic fertilizers per acre;
3. soil insect-killing sterilizes: spring applies phoxim medicine soil 100kg+ carbendazim medicine soil 100kg per acre;
4. female parent processing: llex cornuta lindl female parent base portion branch is wiped out 2/3 by mid-March in spring, only retains 1/3 or so Small stems sprays 20% fenvalerate missible oil, 2000 times of liquid, 1000 times of+50% malathion liquid;
5. top dressing: by the end of April, 10kg/ mus of urea of total Dan Han Liang≤46.4% of application in two dressings by the end of May, applying by the end of June 45% 15kg/ mus of S-based compound fertilizer;
(2) cuttage
1. cutting time: late September;
2. slotting machine prepares: slotting machine wide 1.2m, high 20cm, north-south, after slotting machine applies 2000kg/ mus of decomposed organic fertilizer, Huang Xin Tu ︰ yellow sand=1 ︰ 1(volume ratio of one thickness 8cm of upper berth or so), it is spread fertilizer over the fields after 2% ferrous sulfate is mixed with yellow soil, 100kg is applied per acre, is flattened spare;
3. cuttings clip: choose llex cornuta lindl base portion current year raw coppice shoot, clip length is 1~2cm, diameter is 0.3~ The branch of 0.5cm is cuttings, and the upper and lower clip of cuttings is truncate, and every root cutting fringe has 1 leaf, 1 bud.
4. cuttings processing and cuttage: by the cuttings of step 3. be placed in formula root-growing agent in 2~3s, inserting step 2. insert In bed, blade is inserted into towards east or one, west direction when cuttage;
(3) it is managed after inserting
1. building arched shed: building plastic canopy on slotting machine, cover the sunshade net of obscurity 75%;
2. disinfection: 1 80% mancozeb wettable powder, 400 times of liquid are sprayed after cuttage immediately, later every 20~30 D sprays 1 800 times of 50% carbendazol wettable powder bactericidal liquid, prevents from going rotten, and removes rotten cuttings in time;
3. water and fertilizer management: when slotting machine surface is whitened, in combination with bactericidal liquid moisturizing is sprayed, arch temperature of shed is maintained at 25 DEG C Left and right, relative humidity is up to 80% or more;Cuttings is taken root in 30~40d, after cuttings young leaves at the beginning of 4 months next year is sprouted, throws off plastic canopy And sunshade net, 5~July monthly spray the potassium dihydrogen phosphate of 1 time 3% urea+5%;
Further, phoxim medicine soil and carbendazim medicine soil are respectively will in step (1) the soil insect-killing disinfection 50% phoxim emulsifiable concentrate, 50% carbendazol wettable powder of 0.75kg, which is dissolved in suitable quantity of water, to be mixed native 100kg and is made.
Further, step (2) the cuttage rooting agent is IBA800mg/L+NAA400mg/L+VB15mg/L, mixing are equal It is made after even.
The present invention be by the selection, heavy pruning, base manure of llex cornuta lindl cuttage elite stand application and spray insecticide kill it is overwintering Pest such as wood louse, coccid, and the technical measures such as cutting bed using garden are built, the lignifying of llex cornuta lindl is used in autumn 1 leaf, the 1 bud cuttage of branch, survival rate improve 20%~30% compared with spring, summer cuttage;Elite stand is after rejuvenation childrenization, coppice shoot Development is healthy and strong, and fringe bar Endogenous Hormone Contents in Vitro height is conducive to take root, the soil moisture of Autumn Cutting compared with mercury dropped amplitude slowly, It lags, takes root after cuttage seeding 20d, with the reduction of temperature, the decline of germ infection rate, rooting percent is up to 93.8% or more;It is next Year after cuttings grows young leaves, without being transplanted again, it is higher that cuttings root system by sterile yellow sand matrix extends downwardly into fertility In the ground of garden, by reinforcing water and fertilizer management, growth of cuttage seedlings is rapid, and adaptable, seedling is fast, and autumn can sell.Therefore, our Method compensates for the defects of spring and summer cuttage cuttings susceptible gene is high, survival rate is low, slow growth, and seedling cost is high.
The utility model has the advantages that the present invention in terms of existing technologies, has following advantage:
1) spring and summer cuttings susceptible gene height can be made up using llex cornuta lindl rapid propagation method of the present invention, survived The defects of rate is low, slow growth and seedling cost are high.By elite stand rejuvenation, using garden exempt to transplant, compared with spring and summer cutting survival Rate improves 20~30%, and seedling growth speed is fast, adaptable;The single node cutting of 1 leaf, 1 bud, fringe bar utilization rate improve 60% or more, Significantly reduce the cost of llex cornuta lindl cutting propagation.
2) the present invention provides the llex cornuta lindl rapid propagation method of set of system, pass through female parent selection, processing, pest and disease damage It prevents and treats to slotting machine preparation, cuttings clip and processing, and inserts the technical solutions such as rear water and fertilizer management, a large amount of nothing can be obtained in a short time Property system nursery stock, is the technology progress and innovation of llex cornuta lindl cuttage and seedling culture;This method is without individually construction culture facility, in conjunction with existing There is Seedlings nursery, technical step is simple and practical, and seedling cost is lower, has in the efficient Multiplication and extension application of llex cornuta lindl important Value and meaning.
Specific embodiment
Embodiment 1
A kind of llex cornuta lindl rapid propagation method:
(1) maternal to cultivate
1. female parent selection: selecting triennial no disease and pests harm, the llex cornuta lindl of robust growth is cuttings female parent;
2. soil application: winter 3000~5000kg of application of organic fertilizers per acre;
3. soil insect-killing sterilizes: spring applies phoxim medicine soil 100kg+ carbendazim medicine soil 100kg per acre, and method is point 50% phoxim emulsifiable concentrate of 0.75kg, 50% carbendazol wettable powder are not dissolved in suitable quantity of water and mixes native 100kg and is made;
4. female parent processing: llex cornuta lindl female parent base portion branch is wiped out 2/3 by mid-March in spring, only retains 1/3 or so Small stems sprays 20% fenvalerate missible oil, 2000 times of liquid, 1000 times of+50% malathion liquid;
5. top dressing: by the end of April, 10kg/ mus of urea of total Dan Han Liang≤46.4% of application in two dressings by the end of May, applying by the end of June 45% 15kg/ mus of S-based compound fertilizer;
(2) cuttage
1. cutting time: late September;
2. slotting machine prepares: slotting machine wide 1.2m, high 20cm, north-south, after slotting machine applies 2000kg/ mus of decomposed organic fertilizer, Huang Xin Tu ︰ yellow sand=1 ︰ 1(volume ratio of one thickness 8cm of upper berth or so), it is spread fertilizer over the fields after 2% ferrous sulfate is mixed with yellow soil, 100kg is applied per acre, is flattened spare;
3. cuttings clip: choose llex cornuta lindl base portion current year raw coppice shoot, clip length is 1~2cm, diameter is 0.3~ The branch of 0.5cm is cuttings, and the upper and lower clip of cuttings is truncate, and every root cutting fringe has 1 leaf, 1 bud.
4. cuttings processing and cuttage: the cuttings of step 3. is placed in IBA800mg/L+NAA400mg/L+VB15mg/L matches 2~3s in square root-growing agent, in the slotting machine of inserting step 2., blade is inserted into towards east or one, west direction when cuttage;
(3) it is managed after inserting
1. building arched shed: building plastic canopy on slotting machine, cover the sunshade net of obscurity 75%;
2. disinfection: 1 80% mancozeb wettable powder, 400 times of liquid are sprayed after cuttage immediately, later every 20~30 D sprays 1 800 times of 50% carbendazol wettable powder bactericidal liquid, prevents from going rotten, and removes rotten cuttings in time;
3. water and fertilizer management: when slotting machine surface is whitened, in combination with bactericidal liquid moisturizing is sprayed, arch temperature of shed is maintained at 25 DEG C Left and right, relative humidity is up to 80% or more;Cuttings is taken root in 30~40d, after cuttings young leaves at the beginning of 4 months next year is sprouted, throws off plastic canopy And sunshade net, 5~July monthly spray the potassium dihydrogen phosphate of 1 time 3% urea+5%;Cuttage survival rate is up to 93.8%.
Comparative example 1
(1) cutting time: by the end of March;
(2) slotting machine prepares: with embodiment 1;
(3) cuttings clip: choose llex cornuta lindl tree crown middle and upper part branch, clip length be 1~2cm, diameter be 0.3~ The branch of 0.5cm is cuttings, and the upper and lower clip of cuttings is truncate, and every root cutting fringe has 1 leaf, 1 bud;
(4) cuttings processing and cuttage: with embodiment 1;
(5) it is managed after inserting:
1. building arched shed: with embodiment 1;
2. disinfection: with embodiment 1;
3. water and fertilizer management: when slotting machine surface is whitened, in combination with bactericidal liquid moisturizing is sprayed, arch temperature of shed is maintained at 25 DEG C Left and right, relative humidity is up to 80% or more;70~80d of cuttings is taken root, and as temperature quickly increases, needs to spray water and cool down in time, skewer Motility rate is inserted into up to 56.6%.
Comparative example 2
(1) cutting time: early June;
(2) perlite, vermiculite, peat cutting medium: are mixed and made into cutting medium by the volume ratio of 1 ﹕, 1 ﹕ 1;
(3) cuttings clip: choose llex cornuta lindl tree crown current year sprout spray, clip length be 1~2cm, diameter be 0.3~ The branch of 0.5cm is cuttings, and the upper and lower clip of cuttings is truncate, and every root cutting fringe has 1 leaf, 1 bud;
(4) cuttings processing and cuttage: after the cuttings of step (3) is handled with 1 root-growing agent of embodiment, inserting step (2) In matrix;
(5) it is managed after inserting: the cuttings being plugged in step (4) being placed in full exposure environment, intermittent auto spraying is utilized Device, 1 time spraying every 5~15min, each spray time is 20~30s, adjusts intermittence certainly according to temperature and illumination condition The spraying Time And Frequency of dynamic spraying device, keeps the moisture of blade face, cuttings starts to take root after 60d, and rooting percent reaches It is transplanted after 84.5%, 80~90d, survival rate is up to 70.7%.
The above is only a preferred embodiment of the present invention, it should be pointed out that: for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (4)

1. a kind of llex cornuta lindl rapid propagation method, feature in: the following steps are included:
(1) maternal to cultivate;
(2) cuttage;
1. cutting time: late September;
2. slotting machine prepares: slotting machine wide 1.2m, high 20cm, north-south, after slotting machine applies 2000kg/ mus of decomposed organic fertilizer, upper berth Volume ratio Huang Xin Tu ︰ yellow sand=1 ︰ 1 of one thickness 8cm, spreads fertilizer over the fields after 2% ferrous sulfate is mixed with yellow soil, applies per acre 100kg is flattened spare;
3. cuttings clip: choosing llex cornuta lindl base portion current year raw coppice shoot, clip length is 1~2cm, and diameter is 0.3~0.5cm's Branch is cuttings, and the upper and lower clip of cuttings is truncate, and every root cutting fringe has 1 leaf, 1 bud;
4. cuttings processing and cuttage: the cuttings of step 3. is placed in and is formulated 2~3s in root-growing agent, in the slotting machine of inserting step 2., Blade is inserted into towards east or one, west direction when cuttage;
(3) it is managed after inserting;
1. building arched shed: building plastic canopy on slotting machine, cover the sunshade net of obscurity 75%;
2. disinfection later: spraying 1 80% mancozeb wettable powder, 400 times of liquid after cuttage immediately, sprayed every 20~30 d 1 800 times of 50% carbendazol wettable powder bactericidal liquid, prevents from going rotten, and removes rotten cuttings in time;
3. water and fertilizer management: when slotting machine surface is whitened, in conjunction with spraying bactericidal liquid moisturizing, arch temperature of shed is maintained at 25 DEG C, relatively wet It spends up to 80% or more;Cuttings is taken root in 30~40d, after cuttings young leaves at the beginning of 4 months next year is sprouted, throws off plastic canopy and sunshade net, and 5 ~July monthly sprays the potassium dihydrogen phosphate of 1 time 3% urea+5%.
2. a kind of llex cornuta lindl rapid propagation method according to claim 1, it is characterised in that: step (2) cuttings Root-growing agent is IBA800mg/L+NAA400mg/L+VB15mg/L is made after mixing.
3. a kind of llex cornuta lindl rapid propagation method according to claim 1, it is characterised in that:
The maternal specific steps cultivated in the step (1):
1. female parent selection: selecting 3~5 years raw no disease and pests harms, the llex cornuta lindl of robust growth is cuttings female parent;
2. soil application: winter 3000~5000kg of application of organic fertilizers per acre;
3. soil insect-killing sterilizes: spring applies phoxim medicine soil 100kg+ carbendazim medicine soil 100kg per acre;
4. female parent processing: llex cornuta lindl female parent base portion branch is wiped out 2/3 by mid-March in spring, only retains 1/3 small stems, spray Apply 20% fenvalerate missible oil, 2000 times of liquid, 1000 times of+50% malathion liquid;
5. top dressing: by the end of April, 10kg/ mus of urea of total Dan Han Liang≤46.4% of application in two dressings by the end of May, applying 45% sulfenyl by the end of June Compound fertilizer 15kg/ mus.
4. a kind of llex cornuta lindl rapid propagation method according to claim 3, it is characterised in that: step (1) soil Phoxim medicine soil and carbendazim medicine soil are respectively that 50% phoxim emulsifiable concentrate of 0.75kg, 50% carbendazim is wettable in pesticide and disinfectant Property pulvis, which is dissolved in suitable quantity of water, to be mixed native 100kg and is made.
CN201610631546.6A 2016-08-04 2016-08-04 A kind of llex cornuta lindl rapid propagation method Expired - Fee Related CN106165635B (en)

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CN109156186A (en) * 2018-09-17 2019-01-08 衡东武岳苗木种植专业合作社 A kind of inoculation method of llex cornuta lindl
CN109716943A (en) * 2019-03-13 2019-05-07 江苏省林业科学研究院 A kind of quick-acting root cutting growth methods of red fruit elm

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CN102487719A (en) * 2011-12-30 2012-06-13 南京金埔园林股份有限公司 Simple cutting method for Ilex cornuta Lindli.et Paxt
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Publication number Priority date Publication date Assignee Title
CN101692781A (en) * 2009-10-09 2010-04-14 张冠一 Rapid-forming method of llex cornuta lindl
CN102487719A (en) * 2011-12-30 2012-06-13 南京金埔园林股份有限公司 Simple cutting method for Ilex cornuta Lindli.et Paxt
CN102726189A (en) * 2012-06-29 2012-10-17 句容市仑山花木场 Aquifoliaceae cuttage seedling method
CN104920177A (en) * 2014-03-22 2015-09-23 金寨县冠友盆景园艺有限公司 Training method for aquatic Chinese holly plant

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