CN102498912B - Method for transplanting sugar-cane tissue culture seedlings - Google Patents
Method for transplanting sugar-cane tissue culture seedlings Download PDFInfo
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- CN102498912B CN102498912B CN201110374557.8A CN201110374557A CN102498912B CN 102498912 B CN102498912 B CN 102498912B CN 201110374557 A CN201110374557 A CN 201110374557A CN 102498912 B CN102498912 B CN 102498912B
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Abstract
A method for transplanting sugar-cane tissue culture seedlings is carried out through the method of outdoor one-step transplanting and solves the problem that high cost and large workload are brought to tissue culture production by multi-stage nursery. The method is characterized in that packaged sugar-cane tissue culture seedlings are taken as the material and comprises the steps of laying a transplanting seedling bed, hardening off outdoors, cleaning and disinfecting young seedlings, planting young seedlings, laying a film and a shading net, watering and drug use administration in the early period, fertilizer and water management and vigorous seedling cultivation technology in the later period, and transplanting to large fields or popularization and application. The method can greatly improve the transplanting survival rate of the sugar-cane tissue culture seedlings; the whole production period is short; the tissue culture seedlings can be used for large field production directly; and the method has the characteristics of high efficiency, high quality, quickness and the like and is suitable for the requirements of large-scale and industrial production of sugar-cane tissue culture seedlings.
Description
Technical field
The transplanting method that the present invention relates to a kind of sugar-cane tissue culture seedlings, belongs to field of plant tissue culture technique.
Background technology
Sugarcane (Saccharum.ssp) is the most important sugar [yielding of China, is also most important energy-source plant in the world.The development of sugarcane science and technology has become the new power of Sugarcane Industry development, but the practicality of science and technology and replicability are determining again the practical application of technology.Sugarcane is a kind of vegetative crop, most still taking propagation of seed stock as main on producing.Although the tissue culture technology of sugarcane is ripe already, but in production, application is long-term not enough, also fail large-area popularizing planting sugar-cane tissue culture seedlings in many sugarcanes district because production technology falls behind, therefore, aspect the applying of sugarcane health seedling and new variety of sugarcane, group training Miao Haiwei is fully applied.Sugarcane tissue culture technology also needs to further develop, particularly at group training transplantation of seedlings technical elements.
The transplanting survival rate of sugar-cane tissue culture seedlings affects the quality of group training seedling and the efficiency that tissue is cultivated to a great extent, meanwhile, is also the key technology that group training seedling is applied to Ge Zhe district.A kind of simply, efficiently, fast sugar-cane tissue culture seedlings transplantation technique be at present we in the urgent need to.
Summary of the invention
The present invention proposes a kind of transplanting method of sugar-cane tissue culture seedlings, this method adopts outdoor method of once transplanting packed group of training seedling of sugarcane to carry out, cost height and the large problem of workload that bring to group training production in multistage nursery are reduced, the sugarcane tissue-culture shoot survival percent of heeling in reaches 90%-95%, the sugarcane seedling stalwartness of heeling in, can directly transplant land for growing field crops.The invention has the advantages that simple to operate, with low cost, efficiently quick.Current correlation technique of the present invention have not been reported.
A kind of transplanting method of sugar-cane tissue culture seedlings, taking packed sugar-cane tissue culture seedlings as material, by transplanting the laying in seedbed, outdoor hardening, the cleaning of seedling and sterilization, the plantation of seedling, the laying of film and screened negative, early stage waters and medication management, later stage rich water quality management and strong sprout technology, transplant land for growing field crops or apply.Concrete implementation step is as follows:
(1) laying in transplanting seedbed: carry out the last week in group training transplantation of seedlings, wide as a unit taking 1.2m on the ground at outdoor loess or red soil, length is not limit, its both sides are fenced up with common brick, beyond both sides, down dig the drain ditch of wide 0.3m, dark 10cm, ditch can be spread common brick and be planted seedling and management to facilitate.In plot, unit, spread the thick dedicated substrate mixing of 40cm, matrix components is: 40% loess+40% bagasse matrix+20% river sand.To seedbed disinfection, cover film with 3% carbendazim, for subsequent use after one week.
(2) domestication hardening: packed group of training seedling shifted out from Sterile culture room, be placed on the seedbed of being stamped film in step (1) hardening one week, summer must frame screened negative.
(3) cleaning of seedling and sterilization: the bag seedling in step (2) is cleaned up, remove the dead seedling of part and Huang Ye, the carbendazim with 0.5% soaks 10min, waits to plant.
(4) plantation of seedling: the seedling in step (3) is colonizated on the seedbed of step (1), plants about 1cm dark, water in time normal root water.
(5) laying of film and screened negative: little shed added in seedbed, the both sides, top that film is placed on seedling land, and two ends are opened and are stayed ventilating opening, screened negative to be placed on outside film and by the ventilator hole cover of two ends.
(6) early stage watering managed with medication: after i.e. plantation in week age, open screened negative and film every morning and water once in early days, plantation first day and respectively spraying once with 0.5% carbendazim for the 3rd day.
(7) later stage rich water quality management and strong sprout technology: the later stage planted after one week can thin film, leaves screened negative, within two days, waters once; After two weeks, go screened negative, and to execute for the first time fertile 0.05% composite fertilizer be main, weekly; Cut weekly seedling once; After approximately 20 days, can be affixed by 0.1～0.2% nitrogenous fertilizer or composite fertilizer.
(8) transplant land for growing field crops or apply: sugarcane tissue-culture transplantation of seedlings just can be transplanted land for growing field crops or apply for 40 days above.
The present invention has following outstanding substantive distinguishing features and marked improvement:
1) method of sugar-cane tissue culture seedlings transplantation technique, is characterized in that in outdoor transplanting, instead of transplants in greenhouse, and realization group training seedling is once transplanted, and transplanting process is short, uses manpower and material resources sparingly, efficient and survival rate is high.
2) the present invention by sugarcane tissue culture seedling provisonal planting in specific matrix, matrix components is: 40% loess+40% bagasse matrix+20% river sand, this matrix had both ensured the survival rate that group training seedling is heeled in, and also adapted to balled transplanting land for growing field crops, ensured the survival rate of field-transplanting.
3) the present invention improves the survival rate of heeling in of sugar-cane tissue culture seedlings by practicing the methods such as seedling, cleaning and sterilizing, epiphragma, the shady net of lid, also make sugarcane seedling root growth good by cutting the method such as seedling, fertilising in good time in addition, seedling stalwartness, improves the survival rate in sugarcane seedling transplanting land for growing field crops.The present invention is simple to operation, and cost is low, and sugar-cane tissue culture seedlings transplanting survival rate reaches 90%-95%, is applicable to the needs of batch production large-scale production.
Embodiment
By following examples, the present invention is further elaborated, but content of the present invention is not limited to described scope.
Embodiment mono-
The efficient transplantation technique method of sugar-cane tissue culture seedlings taking packed cloud sugarcane 06-407 sugar-cane tissue culture seedlings as material, was transplanted at the beginning of 5 months, implemented in accordance with the following steps:
1) laying in transplanting seedbed: carry out the last week in group training transplantation of seedlings, wide as a unit taking 1.2m on the ground at outdoor loess or red soil, length is not limit, its both sides are fenced up with common brick, beyond both sides, down dig wide 0.3m, the drain ditch of dark 10cm, ditch can be spread common brick and be planted seedling and management to facilitate.In plot, unit, spread the thick dedicated substrate mixing of 40cm, matrix components is: 40% loess+40% bagasse matrix+20% river sand.To seedbed disinfection, cover film with 3% carbendazim, for subsequent use after one week.
2) domestication hardening: packed group of training seedling shifted out from Sterile culture room, be placed on the seedbed of being stamped film in step (1) hardening one week, summer must frame screened negative.
3) cleaning of seedling and sterilization: the bag seedling in step (2) is cleaned up, remove the dead seedling of part and Huang Ye, the carbendazim with 0.5% soaks 10min, waits to plant.
4) plantation of seedling: the seedling in step (3) is colonizated on the seedbed of step (1), plants about 1cm dark, water in time normal root water.
5) laying of film and screened negative: little shed added in seedbed, the both sides, top that film is placed on seedling land, and two ends are opened and are stayed ventilating opening, screened negative to be placed on outside film and by the ventilator hole cover of two ends.
6) early stage watering managed with medication: after i.e. plantation in week age, open screened negative and film every morning and water once in early days, plantation first day and respectively spraying once with 0.5% carbendazim for the 3rd day.
7) later stage rich water quality management and strong sprout technology: the later stage planted after one week can thin film, leaves screened negative, within two days, waters once; After two weeks, go screened negative, and to execute for the first time fertile 0.05% composite fertilizer be main, weekly; Cut weekly seedling once; After approximately 20 days, be affixed by 0.15% nitrogenous fertilizer or composite fertilizer.
8) transplant land for growing field crops or apply: sugarcane tissue-culture transplantation of seedlings just can be transplanted land for growing field crops or apply for 40 days above.
Embodiment bis-
The efficient transplanting method of sugar-cane tissue culture seedlings, taking packed cloud sugarcane 05-51 sugar-cane tissue culture seedlings as material, transplants mid-July, implements in accordance with the following steps:
1) laying in transplanting seedbed: carry out the last week in group training transplantation of seedlings, wide as a unit taking 1.2m on the ground at outdoor loess or red soil, length is not limit, its both sides are fenced up with common brick, beyond both sides, down dig wide 0.3m, the drain ditch of dark 10cm, ditch can be spread common brick and be planted seedling and management to facilitate.In plot, unit, spread the thick dedicated substrate mixing of 40cm, matrix components is: 40% loess+40% bagasse matrix+20% river sand.To seedbed disinfection, cover film with 3% carbendazim, for subsequent use after one week.
2) domestication hardening: packed group of training seedling shifted out from Sterile culture room, be placed on the seedbed of being stamped film in step (1) hardening one week, set up screened negative.
3) cleaning of seedling and sterilization: the bag seedling in step (2) is cleaned up, remove the dead seedling of part and Huang Ye, the carbendazim with 0.5% soaks 10min, waits to plant.
4) plantation of seedling: the seedling in step (3) is colonizated on the seedbed of step (1), plants about 1cm dark, water in time normal root water.
5) laying of film and screened negative: little shed added in seedbed, the both sides, top that film is placed on seedling land, and two ends are opened and are stayed ventilating opening, screened negative to be placed on outside film and by the ventilator hole cover of two ends.
6) early stage watering managed with medication: after i.e. plantation in week age, open screened negative and film every morning and water once in early days, plantation first day and respectively spraying once with 0.5% carbendazim for the 3rd day.
7) later stage rich water quality management and strong sprout technology: the later stage planted after one week can thin film, leaves screened negative, within two days, waters once; After two weeks, go screened negative, and to execute for the first time fertile 0.05% composite fertilizer be main, weekly; Cut weekly seedling once; After approximately 20 days, be affixed by 0.1% nitrogenous fertilizer or composite fertilizer.
8) transplant land for growing field crops or apply: sugarcane tissue-culture transplantation of seedlings just can be transplanted land for growing field crops or apply for 40 days above.
Claims (1)
1. a transplanting method for sugar-cane tissue culture seedlings, is characterized in that, taking packed cloud sugarcane 06-407 sugar-cane tissue culture seedlings as material, at the beginning of 5 months, transplants, and step is as follows:
(1), transplant the laying in seedbed: carry out the last week in group training transplantation of seedlings, at outdoor loess or red soil, taking 1.2m, wide as a unit, length is not limit on the ground, its both sides is fenced up with common brick, the drain ditch of down digging wide 0.3m, dark 10cm beyond both sides, common brick is spread in ditch; In plot, unit, spread the thick dedicated substrate mixing of 40cm, matrix components is: 40% loess+40% bagasse matrix+20% river sand; To seedbed disinfection, cover film with 3% carbendazim, for subsequent use after one week;
(2), domestication hardening: packed group of training seedling shifted out from Sterile culture room, be placed on the seedbed of being stamped film in step (1) hardening one week, summer must frame screened negative;
(3), cleaning and the sterilization of seedling: the bag seedling in step (2) is cleaned up, remove the dead seedling of part and Huang Ye, the carbendazim with 0.5% soaks 10min, waits to plant;
(4), the plantation of seedling: the seedling in step (3) is colonizated on the seedbed of step (1), and 1cm is dark in plantation, waters in time normal root water;
(5), the laying of film and screened negative: little shed added in seedbed, the both sides, top that film is placed on seedling land, two ends open that to stay ventilating opening, screened negative to be placed on film outer and by the ventilator hole cover of two ends;
(6), early stage watering managed with medication: after plantation, in week age, open screened negative and film every morning and water once, plant first day and respectively spray once with 0.5% carbendazim for the 3rd day;
(7), later stage rich water quality management and strong sprout technology: the later stage plants thin film after a week, leaves screened negative, within two days, waters once; After two weeks, go screened negative, and to execute for the first time fertile 0.05% composite fertilizer be main, weekly; Cut weekly seedling once; After 20 days, be affixed by 0.15% nitrogenous fertilizer or composite fertilizer; Land for growing field crops is transplanted in sugarcane tissue-culture transplantation of seedlings for 40 days above.
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| CN201110374557.8A CN102498912B (en) | 2011-11-23 | 2011-11-23 | Method for transplanting sugar-cane tissue culture seedlings |
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| CN201110374557.8A CN102498912B (en) | 2011-11-23 | 2011-11-23 | Method for transplanting sugar-cane tissue culture seedlings |
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| CN102498912B true CN102498912B (en) | 2014-07-23 |
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| CN103081606B (en) * | 2013-01-30 | 2014-05-07 | 云南省农业科学院甘蔗研究所 | Method for sugarcane spica seed sprouting and sprouted seedling temporary-planting |
| CN104303788B (en) * | 2014-10-25 | 2016-08-17 | 云南省农业科学院甘蔗研究所 | It is a kind of that cane seedlings is floating heels in method |
| CN104770173B (en) * | 2015-04-13 | 2017-05-17 | 云南省农业科学院甘蔗研究所 | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings |
| CN104756745B (en) * | 2015-04-27 | 2017-09-19 | 中国热带农业科学院热带生物技术研究所 | A kind of sugarcane detoxication original seed seedling exempts careless method for transplanting |
| CN105191610A (en) * | 2015-08-24 | 2015-12-30 | 太仓市丰缘农场专业合作社 | Method for high-yield planting of sugarcanes |
| CN106332648A (en) * | 2016-08-30 | 2017-01-18 | 广西壮族自治区农业科学院甘蔗研究所 | Single-plant temporary planting method of sugarcane tissue culture rooting bottle seedling |
| CN107047277A (en) * | 2017-05-03 | 2017-08-18 | 蚌埠市宝煦家庭农场 | A kind of method for culturing seedlings for improving sugarcane germination percentage |
| CN107980531A (en) * | 2017-12-25 | 2018-05-04 | 洛阳名力科技开发有限公司 | A kind of method for transplanting of sugarcane tissue-culture nursery |
| CN113545268B (en) * | 2021-08-13 | 2022-09-09 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
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