CN103503686A - Rapid breeding method for grape seedlings - Google Patents
Rapid breeding method for grape seedlings Download PDFInfo
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- CN103503686A CN103503686A CN201310502083.XA CN201310502083A CN103503686A CN 103503686 A CN103503686 A CN 103503686A CN 201310502083 A CN201310502083 A CN 201310502083A CN 103503686 A CN103503686 A CN 103503686A
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Abstract
The invention belongs to the plant non-test-tube rapid breeding technology, and relates to a rapid breeding method for grape seedlings. The method comprises the following steps: A. selecting and pre-treating an ex-vivo material: selecting stem buds from sprouts on stolons of the grape seedlings, disinfecting the notch through carbendazim, and soaking through naphthylacetic acid; B. permanently planting the ex-vivo material: inserting the ex-vivo material into a pearlstone substrate; C. performing the rooting period management of the ex-vivo material: controlling the plant growth environment condition through an automatic control system; if new roots begin to bud, spraying a nutrient solution once per 24 hours, and obtaining the length of the new root is 3 to 5 cm through visual-estimating; D. performing seedling refining period management of the ex-vivo material: detecting that 1 to 2 pieces of blades are grown at the upper part of the ex-vivo material, and then ensuring the ex-vivo material to enter a nursery stock period; E. performing nursery stock period management: transplanting the ex-vivo material into a culture container or a culture ground bed with conventional grape culture soil, and obtaining commodity seedlings after 18 to 25 days growing. The method greatly improves the breeding speed and breeding survival rate, and is suitable for industrialized implementation of large scale rapid breeding better.
Description
Technical field
The invention belongs to the non-tube rapid propagation technology of plant, relate to a kind of fast breeding method of grape seedlings.
Background technology
Existing grape seedlings mating system, mainly contains tissue culture technique and traditional branch cutting technology.But the cutting propagation cycle is long, and survival rate is low, and is difficult to realize and grows seedlings on a large scale.Tissue culture technique is used for cultivating nontoxic seedling (generation seedling), is only applicable to scientific experiment, and cost is high, is difficult to large-scale production.
Summary of the invention
Technical problem to be solved by this invention is, provides a kind of and can increase substantially the speed of breeding and breed survival rate, and be applicable to the fast breeding method of the grape seedlings of growing seedlings on a large scale.
The fast breeding method of grape seedlings of the present invention comprises the following steps:
Selected and the pretreatment of A, To body material: selectedly on grape branch grow fine, disease-free shoot then, intercepting is with the branch of 1-2 gemma, lower cut is miter angle, the carbendazim sterilization of 50～1000 times of dilute with waters for otch, then soak 25～30 minutes with the methyl α-naphthyl acetate of 50～1000 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 2000～3000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～29 ℃ of matrix and air themperatures, matrix relative moisture is 60～68%, relative air humidity is 65～70%; From new root eruption, every 24 hours spray nutritious liquors 1 time (potassium dihydrogen phosphate, mass concentration 5 ‰-6 ‰), estimate new root length 3～5cm, enter the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 2000～3000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～29 ℃ of matrix and air themperatures, matrix relative moisture is 60～68%, relative air humidity is 65～70%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional viticulture soil,
conventional cultivation management 1within 8～25 days, become commercial seedling.
The present invention compares and has the following advantages with existing grape seedlings raising technology:
1, increased substantially the speed of breeding and bred survival rate.Through reality, implement test, foreshorten to 30-35 days breeding cycle; By the cutting propagation survival rate 60～75% of prior art, bring up to more than 98%.
2, be applicable to growing seedlings on a large scale.The present invention can control rearing condition by intelligence control system, compare with the artificial cutting propagation of prior art, realize automation, intellectuality, precision and the high efficiency management of seedling raising process, be more applicable to the industrialized implementation of extensive fast seedling growing.
Embodiment
The fast breeding method of the grape seedlings of the embodiment of the present invention comprises the following steps:
Selected and the pretreatment of A, To body material: selectedly on grape branch grow fine, disease-free shoot then, intercepting is with the branch of 1-2 gemma, lower cut is miter angle, the carbendazim sterilization of 1000 times of dilute with waters for otch, then soak 30 minutes with the methyl α-naphthyl acetate of 500 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: intensity of illumination 2500Lux, light application time 12 hours/day, matrix matrix and air themperature, daytime, temperature was 28 ℃, and night, temperature was 19.5 ℃, and matrix relative moisture is 62%, relative air humidity is 65%; From new root eruption, every 24 hours spray nutritious liquors 1 time (potassium dihydrogen phosphate, mass concentration 5 ‰-6 ‰), estimate new root length 3～5cm, enter the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 2500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 28 ℃, and night, temperature was 19.5 ℃, and matrix relative moisture is 60%, relative air humidity is 65%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional viticulture soil,
conventional cultivation management 1within 8～25 days, become commercial seedling.
The grape seedlings of embodiment of the present invention cultivation, through test, breeding cycle, 30-35 days, bred survival rate more than 98%.
Claims (4)
1. the fast breeding method of a grape seedlings comprises the following steps:
Selected and the pretreatment of A, To body material: selectedly on grape branch grow fine, disease-free shoot then, intercepting is with the branch of 1-2 gemma, lower cut is miter angle, the carbendazim sterilization of 50～1000 times of dilute with waters for otch, then soak 25～30 minutes with the methyl α-naphthyl acetate of 50～1000 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 2000～3000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～29 ℃ of matrix and air themperatures, matrix relative moisture is 60～68%, relative air humidity is 65～70%; From new root eruption, within every 24 hours, spray nutritious liquor is 1 time, estimates new root length 3～5cm, enters the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 2000～3000Lux that environment is controlled parameter, light application time 10～14 hours/day, 18～29 ℃ of matrix and air themperatures, matrix relative moisture is 60～68%, relative air humidity is 65～70%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional viticulture soil,
conventional cultivation management 1within 8～25 days, become commercial seedling.
2. the fast breeding method of grape seedlings according to claim 1, is characterized in that: the nutrient solution in step C and D is the potassium dihydrogen phosphate of mass concentration 5 ‰-6 ‰.
3. the fast breeding method of grape seedlings according to claim 1, it is characterized in that: in step C, it is intensity of illumination 2500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 28 ℃, and night, temperature was 19.5 ℃, and matrix relative moisture is 65%, relative air humidity is 70%.
4. the fast breeding method of grape seedlings according to claim 1, is characterized in that: it is intensity of illumination 3500Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 28 ℃, and night, temperature was 19.5 ℃, and matrix relative moisture is 70%, relative air humidity is 75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310502083.XA CN103503686A (en) | 2013-10-23 | 2013-10-23 | Rapid breeding method for grape seedlings |
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| CN201310502083.XA CN103503686A (en) | 2013-10-23 | 2013-10-23 | Rapid breeding method for grape seedlings |
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| CN103503686A true CN103503686A (en) | 2014-01-15 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145269A (en) * | 2015-07-12 | 2015-12-16 | 青岛乐义现代农业科技示范基地有限公司 | Efficient seedling cultivating method for leafbuds of Yunshan 2# grape novel variety |
| CN106718749A (en) * | 2016-12-16 | 2017-05-31 | 福建百卉花艺有限公司 | Water culture method |
| CN107258461A (en) * | 2017-07-20 | 2017-10-20 | 崔舜� | A kind of fast numerous integrated technology of grape pot |
| CN107646505A (en) * | 2017-10-27 | 2018-02-02 | 金寨县绿丰园葡萄种植专业合作社 | A kind of breeding method of grape |
| CN108966880A (en) * | 2018-08-13 | 2018-12-11 | 紫云自治县高原红种植农民专业合作社 | A kind of mating system of high mountain Vitis davidii Foex |
| CN110402816A (en) * | 2019-07-25 | 2019-11-05 | 河北农业大学 | System for increasing transplanting seedling emergence amount of test-tube grape seedlings and application method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0398518A (en) * | 1989-09-12 | 1991-04-24 | Kyowa Kk | Nutritive solution culture of tree and device therefor |
| US20100064582A1 (en) * | 2005-10-24 | 2010-03-18 | Jeong Woo-Choon | Method for mass production of seedling of seed potato |
| CN102369868A (en) * | 2010-08-24 | 2012-03-14 | 卢磊 | Cuttage seedling-cultivating technology of grape electric heating root-promoting container |
| CN102630544A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Method for planting Picea koraiensis |
| CN102696441A (en) * | 2012-05-29 | 2012-10-03 | 句容市张小虎葡萄专业合作社 | Grape cuttage seedling method |
| CN102742484A (en) * | 2011-10-31 | 2012-10-24 | 新疆林业科学院 | Breeding method of good grapes for brewing ice wine |
| CN103070002A (en) * | 2013-02-05 | 2013-05-01 | 镇江市丹徒区紫杉生态农业园 | Schefflera octophylla non-test tube rapid propagation method |
| CN103168608A (en) * | 2013-04-18 | 2013-06-26 | 曾小虎 | Grape cuttage root-forcing growing method |
-
2013
- 2013-10-23 CN CN201310502083.XA patent/CN103503686A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0398518A (en) * | 1989-09-12 | 1991-04-24 | Kyowa Kk | Nutritive solution culture of tree and device therefor |
| US20100064582A1 (en) * | 2005-10-24 | 2010-03-18 | Jeong Woo-Choon | Method for mass production of seedling of seed potato |
| US7877928B2 (en) * | 2005-10-24 | 2011-02-01 | Koesan County | Apparatus for use with a method for mass production of seedling of seed potato |
| CN102369868A (en) * | 2010-08-24 | 2012-03-14 | 卢磊 | Cuttage seedling-cultivating technology of grape electric heating root-promoting container |
| CN102742484A (en) * | 2011-10-31 | 2012-10-24 | 新疆林业科学院 | Breeding method of good grapes for brewing ice wine |
| CN102630544A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Method for planting Picea koraiensis |
| CN102696441A (en) * | 2012-05-29 | 2012-10-03 | 句容市张小虎葡萄专业合作社 | Grape cuttage seedling method |
| CN103070002A (en) * | 2013-02-05 | 2013-05-01 | 镇江市丹徒区紫杉生态农业园 | Schefflera octophylla non-test tube rapid propagation method |
| CN103168608A (en) * | 2013-04-18 | 2013-06-26 | 曾小虎 | Grape cuttage root-forcing growing method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145269A (en) * | 2015-07-12 | 2015-12-16 | 青岛乐义现代农业科技示范基地有限公司 | Efficient seedling cultivating method for leafbuds of Yunshan 2# grape novel variety |
| CN106718749A (en) * | 2016-12-16 | 2017-05-31 | 福建百卉花艺有限公司 | Water culture method |
| CN107258461A (en) * | 2017-07-20 | 2017-10-20 | 崔舜� | A kind of fast numerous integrated technology of grape pot |
| CN107646505A (en) * | 2017-10-27 | 2018-02-02 | 金寨县绿丰园葡萄种植专业合作社 | A kind of breeding method of grape |
| CN108966880A (en) * | 2018-08-13 | 2018-12-11 | 紫云自治县高原红种植农民专业合作社 | A kind of mating system of high mountain Vitis davidii Foex |
| CN110402816A (en) * | 2019-07-25 | 2019-11-05 | 河北农业大学 | System for increasing transplanting seedling emergence amount of test-tube grape seedlings and application method thereof |
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Application publication date: 20140115 |