CN103503685A - Rapid propagation method for cajuput seedlings - Google Patents
Rapid propagation method for cajuput seedlings Download PDFInfo
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- CN103503685A CN103503685A CN201310501812.XA CN201310501812A CN103503685A CN 103503685 A CN103503685 A CN 103503685A CN 201310501812 A CN201310501812 A CN 201310501812A CN 103503685 A CN103503685 A CN 103503685A
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Abstract
The invention belongs to plant non-test tube rapid propagation technology, and relates to a rapid propagation method for cajuput seedlings. The method comprises the following steps: A, the selection and pre-treatment of in-vitro materials: selecting stem buds from sprouts on stolons of cajuput seedlings, disinfecting incisions with carbendazim, and soaking in naphthylacetic acid; B, the field planting of the in-vitro materials: inserting the in-vitro materials into a pearlstone substrate; C, the rooting period management of the in-vitro materials: controlling the plant growing environmental condition through an automatic control system, from the beginning of the budding of a new root, spraying a nutrient solution once per 24 hours until the length of the new root is 3-5 cm through visual inspection; D, the hardening-seedling period management of the in-vitro materials: entering the nursery stock period after detecting that 1-2 leaves are grown at the upper parts of the in-vitro materials; E, the nursery stock period management: transplanting into a cultivation container or a cultivation ground bed having the conventional cajuput cultivation soil, so that commercial seedlings can be obtained after 8-10 days. According to the invention, the propagation speed and propagation survival rate are greatly improved, and the rapid propagation method is more suitable for the industrial implementation of large-scale rapid seedling culture.
Description
Technical field
The invention belongs to the non-tube rapid propagation technology of plant, relate to a kind of fast breeding method of cajaput seedling.
Background technology
Existing cajaput seedling mating system, mainly contains tissue culture technique and traditional branch cutting technology.But the cutting propagation cycle is long, and survival rate is low, and is difficult to realize and grows seedlings on a large scale.Tissue culture technique is used for cultivating nontoxic (generation seedling), is only applicable to scientific experiment, and cost is high, is difficult to large-scale production.
Summary of the invention
Technical problem to be solved by this invention is, provides a kind of and can increase substantially the speed of breeding and breed survival rate, and be applicable to the fast breeding method of the cajaput seedling of growing seedlings on a large scale.
The fast breeding method of cajaput seedling of the present invention comprises the following steps:
Selected and the pretreatment of A, To body material: intercepting is with the branch of 1-2 sheet leaf on ripe cajaput plant, height 4-6cm, dry, the carbendazim sterilization of 50～1000 times of dilute with waters for otch, then soak 4～6 minutes with the methyl α-naphthyl acetate of 50～1000 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 6000～8000Lux that environment is controlled parameter, light application time 10～14 hours/day, 16～27 ℃ of matrix and air themperatures, matrix relative moisture is 45～55%, relative air humidity is 55～60%; From new root eruption, every 24 hours spray nutritious liquors 1 time (potassium dihydrogen phosphate, mass concentration 5 ‰-6 ‰), estimate new root length 3～5cm, enter the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 6000～8000Lux that environment is controlled parameter, light application time 10～14 hours/day, 16～27 ℃ of matrix and air themperatures, matrix relative moisture is 45～55%, relative air humidity is 55～60%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional cajaput cultivating soil,
conventional cultivation management 8within～10 days, become commercial seedling.
The present invention compares and has the following advantages with existing cajaput seedling raising technology:
1, increased substantially the speed of breeding and bred survival rate.Through reality, implement test, foreshorten to 15-20 days breeding cycle; Breeding survival rate brings up to more than 98%.
2, be applicable to growing seedlings on a large scale.The present invention can control rearing condition by intelligence control system, compare with the artificial cutting propagation of prior art, realize automation, intellectuality, precision and the high efficiency management of seedling raising process, be more applicable to the industrialized implementation of extensive fast seedling growing.
Embodiment
The fast breeding method of the cajaput seedling of the embodiment of the present invention comprises the following steps:
Selected and the pretreatment of A, To body material: selectedly on cajaput branch grow fine, disease-free shoot then, intercepting is with the branch of 1-2 gemma, lower cut is miter angle, the carbendazim sterilization of 1000 times of dilute with waters for otch, then soak 5 minutes with the methyl α-naphthyl acetate of 500 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 7Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, night, temperature was 16 ℃, and matrix relative moisture is 50%, relative air humidity is 55%; From new root eruption, every 24 hours spray nutritious liquors 1 time (potassium dihydrogen phosphate, mass concentration 5 ‰-6 ‰), estimate new root length 3～5cm, enter the hardening phase;
The hardening period management of D, To body material: it is intensity of illumination 7000Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, and night, temperature was 16 ℃, and matrix relative moisture is 50%, relative air humidity is 55%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional cajaput cultivating soil,
conventional cultivation management 8within～10 days, become commercial seedling.
The cajaput seedling of embodiment of the present invention cultivation, through test, breeding cycle, 15-20 days, bred survival rate more than 98%.
Claims (4)
1. the fast breeding method of a cajaput seedling comprises the following steps:
Selected and the pretreatment of A, To body material: intercepting is with the branch of 1-2 sheet leaf on ripe cajaput plant, height 4-6cm, dry, the carbendazim sterilization of 50～1000 times of dilute with waters for otch, then soak 4～6 minutes with the methyl α-naphthyl acetate of 50～1000 times of dilute with waters;
The field planting of B, To body material: aforementioned pretreated To body material is inserted to pearlite interstitial substance, and To body material gos deep into matrix 3～5cm, is as the criterion with phase non-overlapping copies;
The period management of taking root of C, To body material: plant strain growth environmental condition is controlled by automatic control system, it is intensity of illumination 6000～8000Lux that environment is controlled parameter, light application time 10～14 hours/day, 16～27 ℃ of matrix and air themperatures, matrix relative moisture is 45～55%, relative air humidity is 55～60%; From new root eruption, within every 24 hours, spray nutritious liquor is 1 time, estimates new root length 3～5cm, enters the hardening phase
The hardening period management of D, To body material: it is intensity of illumination 6000～8000Lux that environment is controlled parameter, light application time 10～14 hours/day, 16～27 ℃ of matrix and air themperatures, matrix relative moisture is 45～55%, relative air humidity is 55～60%; The sprinkling of nutrient solution is spaced apart 24 hours and sprays 2 times, detects after To body material top grows 1～2 blade and enters the nursery stock phase;
E, nursery stock period management: will complete the To body material of hardening phase, again transplant to having in the cultivating container or cultivation groundbed of conventional cajaput cultivating soil,
conventional cultivation management 8within～10 days, become commercial seedling.
2. the fast breeding method of cajaput seedling according to claim 1, is characterized in that: the nutrient solution in step C and D is the potassium dihydrogen phosphate of mass concentration 5 ‰-6 ‰.
3. the fast breeding method of cajaput seedling according to claim 1, is characterized in that: in step C, it is intensity of illumination 7000Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, night, temperature was 16 ℃, and matrix relative moisture is 50%, relative air humidity is 55%.
4. the fast breeding method of cajaput seedling according to claim 1, is characterized in that: it is intensity of illumination 7000Lux that environment is controlled parameter, light application time 12 hours/day, matrix and air themperature, daytime, temperature was 26 ℃, and night, temperature was 16 ℃, and matrix relative moisture is 50%, relative air humidity is 55%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310501812.XA CN103503685A (en) | 2013-10-23 | 2013-10-23 | Rapid propagation method for cajuput seedlings |
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| CN201310501812.XA CN103503685A (en) | 2013-10-23 | 2013-10-23 | Rapid propagation method for cajuput seedlings |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938179A (en) * | 2015-06-10 | 2015-09-30 | 苏州苏农园艺景观有限公司 | Planting method for cajuput flowers |
| CN105075589A (en) * | 2015-07-30 | 2015-11-25 | 黄旭胜 | Tomato seedling rapid propagation method |
| CN106576755A (en) * | 2016-11-24 | 2017-04-26 | 蚌埠市宗洼草鸡养殖农民专业合作社 | Jade plant cuttage breeding method in high survival rate |
| CN106922497A (en) * | 2015-12-30 | 2017-07-07 | 镇江市丹徒区紫杉生态农业园 | A kind of ciltivating process of cinnamomum camphora |
| CN113875537A (en) * | 2021-11-04 | 2022-01-04 | 华南农业大学 | Method for promoting branches of cajuput trees by high-temperature treatment |
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| CN1806512A (en) * | 2005-11-25 | 2006-07-26 | 湖南省林业科学院 | E. tirucalli cuttage propagation and tissue-culturing quick-propagation method |
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| CN102630544A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Method for planting Picea koraiensis |
| CN103070002A (en) * | 2013-02-05 | 2013-05-01 | 镇江市丹徒区紫杉生态农业园 | Schefflera octophylla non-test tube rapid propagation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH03191726A (en) * | 1989-12-19 | 1991-08-21 | Tabai Espec Corp | Nutriculture system of plant |
| CN1187933A (en) * | 1997-01-13 | 1998-07-22 | 丁庆 | Cultivation method for crassula perforate, cotyledon malacophylla, monochoria vaginalis fordia cauliflora |
| CN1806512A (en) * | 2005-11-25 | 2006-07-26 | 湖南省林业科学院 | E. tirucalli cuttage propagation and tissue-culturing quick-propagation method |
| JP2007175018A (en) * | 2005-12-28 | 2007-07-12 | Yasuhisa Yamasato | Rooftop greening plant cultivation system |
| RU2010123139A (en) * | 2010-06-07 | 2011-12-20 | Федеральное государственное образовательное учреждение высшего профессионального образования "Оренбургский государственный аграрны | METHOD FOR GROWING THE STAMP FORM OF THE SVERDLOVSK SINFUL WEAPING WILLOW |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938179A (en) * | 2015-06-10 | 2015-09-30 | 苏州苏农园艺景观有限公司 | Planting method for cajuput flowers |
| CN105075589A (en) * | 2015-07-30 | 2015-11-25 | 黄旭胜 | Tomato seedling rapid propagation method |
| CN106922497A (en) * | 2015-12-30 | 2017-07-07 | 镇江市丹徒区紫杉生态农业园 | A kind of ciltivating process of cinnamomum camphora |
| CN106576755A (en) * | 2016-11-24 | 2017-04-26 | 蚌埠市宗洼草鸡养殖农民专业合作社 | Jade plant cuttage breeding method in high survival rate |
| CN113875537A (en) * | 2021-11-04 | 2022-01-04 | 华南农业大学 | Method for promoting branches of cajuput trees by high-temperature treatment |
| CN113875537B (en) * | 2021-11-04 | 2022-05-13 | 华南农业大学 | Method for promoting branches of cajuput trees by high-temperature treatment |
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Application publication date: 20140115 |