CN113099971A - Method for promoting advanced flowering of industrial hemp and application thereof - Google Patents

Method for promoting advanced flowering of industrial hemp and application thereof Download PDF

Info

Publication number
CN113099971A
CN113099971A CN202110467882.2A CN202110467882A CN113099971A CN 113099971 A CN113099971 A CN 113099971A CN 202110467882 A CN202110467882 A CN 202110467882A CN 113099971 A CN113099971 A CN 113099971A
Authority
CN
China
Prior art keywords
industrial hemp
seedlings
industrial
flowering
hemp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202110467882.2A
Other languages
Chinese (zh)
Other versions
CN113099971B (en
Inventor
刘立军
李明月
田瑞丰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huazhong Agricultural University
Original Assignee
Huazhong Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huazhong Agricultural University filed Critical Huazhong Agricultural University
Priority to CN202110467882.2A priority Critical patent/CN113099971B/en
Publication of CN113099971A publication Critical patent/CN113099971A/en
Application granted granted Critical
Publication of CN113099971B publication Critical patent/CN113099971B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14—Measures for saving energy, e.g. in green houses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a method for promoting advanced flowering of industrial hemp and application thereof, wherein the method comprises the following steps: after the industrial hemp is cultured until the second pair of true leaves is unfolded, the industrial hemp is transplanted into a phytotron for culture, and the illumination intensity of a light supplement lamp in the phytotron is adjusted as follows: 30 to 180. mu. mol/m‑2·s‑1The photoperiod is 12-14 h/10-12 h, and the temperature is 25 +/-1 ℃; further, 200mg/L of 6-BA + 50-200 mg/L of gibberellin is sprayed on the industrial hemp seedlings. Aiming at the problems of difficult propagation and long growth period of industrial hemp in winter under natural conditions, the invention changes the illumination intensity by manually regulating and controlling the luminous environment, and effectively shortens the growth period of the industrial hemp by matching with the application of the plant growth regulator. The time from sowing to flowering period is only 50 days, thereby realizing early flowering and fructification and achieving the purpose of shortening the growth of industrial hempThe method has the advantages of low cost, simple operation, high success rate, and extremely high application value and economic benefit.

Description

Method for promoting advanced flowering of industrial hemp and application thereof
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a method for promoting advanced flowering of industrial hemp and application thereof.
Background
Cannabis sativa L.is an annual herb plant of Cannabis of Urticales, Moraceae, and includes industrial, medicinal and recreational Cannabis. In recent years, industrial hemp is widely applied in various fields such as textile, construction, medical use, daily use chemicals and the like, and the fiber and the secondary metabolite of the industrial hemp have high economic value. At present, the planting of industrial hemp in China is mostly concentrated in Yunnan and Heilongjiang. The industrial hemp is lucidie, and the optimal growth temperature is 25-30 ℃. The light intensity is insufficient in Yunnan winter, the temperature in Heilongjiang winter is too low, and the winter propagation of the industrial marihuana in two places is restricted under the influence of two factors of the light intensity and the temperature. Therefore, there is a need for artificially controlling the growth environment of industrial hemp to solve the problems of difficult propagation of industrial hemp in winter and long growth period under natural conditions.
The LED light supplement lamp is the most common lighting facility for indoor plant cultivation, has adjustable spectrum, high photoelectric conversion rate, large space utilization rate, energy conservation, environmental protection and long service life, and is a more ideal indoor artificial light source compared with incandescent lamps, high-voltage sodium lamps and fluorescent lamps. The plant growth regulator is natural or artificial organic compound with growth and development regulating effect similar to natural plant hormone. Is widely applied to the research and production of plants.
Disclosure of Invention
The invention provides a method for promoting the industrial hemp to bloom in advance aiming at the technical problems of difficult propagation of the industrial hemp in winter and long growth period under natural conditions, and the method effectively shortens the growth period of the industrial hemp by manually regulating and controlling the light environment, changing the illumination intensity and simultaneously cooperatively applying a plant growth regulator. The period from sowing to flowering lasts for 50 days, the flowering and fructification are realized in advance, the purpose of shortening the growth period of the industrial hemp is achieved, and the method has the advantages of low cost, simple operation, high success rate and extremely high application value and economic benefit.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method of promoting premature flowering of industrial cannabis, the method comprising: after the industrial hemp is cultured until the second pair of true leaves is unfolded, the industrial hemp is transplanted into a phytotron for culture, and the illumination intensity of a light supplement lamp in the phytotron is adjusted as follows: 30 to 180. mu. mol/m-2·s-1The photoperiod is 12-14 h/10-12 h, and the temperature is 25 +/-1 ℃.
Furthermore, the illumination intensity of the light supplement lamp in the method is 130 mu mol.m-2·s-1。
Further, the method further comprises: and spraying 200mg/L6-BA + 50-200 mg/L gibberellin to the seedlings of the industrial hemp.
Further, 200mg/L of 6-BA +100mg/L of gibberellin is sprayed to the seedlings of the industrial hemp.
Further, after the fourth pair of true leaves is developed after the seedlings grow, 6-BA + gibberellin is sprayed to the leaf surfaces once every 10 days.
Further, the distance between the light supplementing lamp and the top end of the industrial hemp is 50 cm.
Further, industrial hemp seeds which are full in shape and uniform in size are taken, soaked in clear water for 16-24 hours for germination acceleration, then sowed in a seedling tray, watered thoroughly, placed in an artificial cultivation room for seedling cultivation, transplanted in a flowerpot after a second pair of true leaves is unfolded, watered, and the seedlings are revived for one day under the dark condition, and then transplanted in an artificial climate room for cultivation.
Further, the illumination intensity in the artificial culture room is adjusted to be 100 mu mol.m in the seedling culture process-2·s-1The photoperiod is 16h/8h, and the temperature is 25 ℃.
Furthermore, the growth environment is free from plant diseases and insect pests and sufficient in moisture in the seedling raising process.
The invention also provides application of the method for promoting the industrial hemp to bloom in advance in indoor cultivation of the industrial hemp.
Compared with the prior art, the invention has the beneficial effects that: aiming at the technical problems that the industrial hemp is difficult to propagate under natural conditions in winter, the growth period is long, and the industrial production is hindered, the indoor cultivation is adopted, the hemp growth environment is artificially regulated, and the illumination and the plant growth regulator are matched for use, and the illumination intensity and the dosage of the plant growth regulator are optimized, so that the synergistic effect is exerted, the advanced flowering of the industrial hemp is effectively promoted, and the growth period of the industrial hemp is shortened. The method only lasts for 50 days from sowing to flowering, shortens the growth period of nearly two months compared with the growth under natural conditions, and achieves the purposes of advancing flowering and fructification and shortening the breeding period. Meanwhile, the method disclosed by the invention is simple to operate, low in investment cost and low in requirement on operators, saves time cost and labor cost, improves production efficiency, and has extremely high production application value and economic benefit.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention takes industrial hemp seeds 'Longma No. 3' as an example to verify the effect of the method, and the specific operation is as follows:
selecting industrial hemp seeds 'Longma No. 3' with luster, full shape and uniform size in 24 days 10 months in 2020, soaking the seeds in clear water for 16-24 h, and carrying out germination acceleration, wherein the clear water is replaced for 1-2 times.
Sowing the soaked seeds in a deep seedling-raising tray in 2020, 10 months and 25 days, watering thoroughly, and placing in a place with light intensity of 100 μmol · m-2·s-1And the photoperiod is 16h/8h, the artificial culture room at the temperature of 25 ℃ is used for seedling, the conventional agricultural management measures are adopted during seedling culture, the growth environment is ensured to be free of plant diseases and insect pests, the moisture is sufficient, and the seedlings are thinned and supplemented after the seedlings grow to the first pair of true leaves and are unfolded.
And transplanting the seedlings into a double-color flowerpot with the specification of d being 20cm after the seedlings grow to the second pair of true leaves, selecting a brand-new seedling culture substrate (purchased from Wuhan Star Biotechnology limited) for the pot culture soil, and watering thoroughly after transplanting.
The transplanted industrial hemp seedlings are revived for one day under the dark condition of a climatic chamber at the temperature of 25 ℃, after the seedlings are fully adapted to the environment of the potted plant, the potted plant is placed in the climatic chamber provided with an LED quantum plate light supplement lamp (the model is MOSO LSV-320B036) for culture, and the industrial hemp seedlings are cultured in the climatic chamberThe illumination intensity is adjusted to 130 mu mol.m-2·s-1The photoperiod is 14h/10h, the room temperature is 25 +/-1 ℃, and the distance between the light source and the topmost end of the plant is kept at 50cm in the growth process of the industrial hemp. And spraying 200mg/L6-BA +100mg/L Gibberellin (GA) once every 10 days after the seedlings grow to the fourth pair of true leaves3) The mixed solution of (1) is sprayed on the leaf surfaces until the leaf surfaces are wet. Meanwhile, the growth period of the industrial hemp is matched with a conventional cultivation management method during the growth period of the industrial hemp, so that the growth environment is free of plant diseases and insect pests, the soil moisture is sufficient, and the growth period of the industrial hemp 'Longma No. 3' is observed and recorded.
Example 2
The present embodiment is different from embodiment 1 in that: in this example, the transplanted potted seedlings were cultivated in a climatic chamber equipped with an LED light supplement lamp (MOSO LSV-320B036) and the illumination intensity was adjusted to 180. mu. mol. m-2·s-1。
Example 3
The present embodiment is different from embodiment 1 in that: in this example, the transplanted potted seedlings were cultivated in a climatic chamber equipped with an LED light supplement lamp (MOSO LSV-320B036) and the illumination intensity was adjusted to 80. mu. mol. m-2·s-1。
Example 4
The present embodiment is different from embodiment 1 in that: in this example, the transplanted potted seedlings were cultivated in a climatic chamber equipped with an LED light supplement lamp (model: MOSO LSV-320B036) and the illumination intensity was adjusted to 30. mu. mol. m-2·s-1。
Example 5
The present embodiment is different from embodiment 1 in that: in the embodiment, 200mg/L6-BA +50mg/L Gibberellin (GA) is sprayed once every 10 days after the seedlings grow to the fourth pair of true leaves are unfolded3) The mixed solution of (1) is sprayed on the leaf surfaces until the leaf surfaces are wet.
Example 6
The present embodiment is different from embodiment 1 in that: in the embodiment, after the seedlings grow to the fourth pair of true leaves are unfolded, the seedling is sprayed with one in every 10 daysSub-200 mg/L6-BA +200mg/L Gibberellin (GA)3) The mixed solution of (1) is sprayed on the leaf surfaces until the leaf surfaces are wet.
Example 7
The present embodiment is different from embodiment 1 in that: in this example, no plant growth regulator was sprayed during the cultivation.
Comparative example 1
This comparative example differs from example 1 in that: in the comparative example, after the seedlings grow to the second pair of true leaves and are transplanted into flowerpots, the seedlings are not placed in a phytotron for culture, but are directly placed under natural conditions for conventional culture.
Comparative example 2
This comparative example differs from example 1 in that: the comparative example was sprayed with 200mg/L6-BA solution every 10 days after the seedlings grew to the fourth true leaves were expanded, and sprayed on the leaf surfaces until the leaf surfaces were wet.
Evaluation method
And (3) transplanting the 'Longma No. 3' seedlings at the same time in 10 months and 30 days, respectively culturing in different environments described in examples 1-7 and comparative examples 1-2, and observing and counting the growth period of the 'Longma No. 3' seedlings in different culture environments, namely the date of entering different growth stages. The statistics are shown in Table 1, where the numbers represent dates, e.g., 10.30 for 10 months and 30 days.
TABLE 1 statistics of growth period of Longma No. 3 under different growth environments
Figure BDA0003044819000000051
Figure BDA0003044819000000061
In conclusion, the LED quantum plate is used as an indoor light source, the illumination intensity of the industrial hemp growth environment is manually regulated, and the plant growth regulator is applied in a matched manner, so that the synergistic effect is achieved, and the flowering phase of the industrial hemp is effectively extracted. In which, in natural conditions (comparative example 1), the time from sowing to the end of flowering of industrial hemp 'Longma No. 3' is about 90 days, whereas the time from sowing to the end of flowering of industrial hemp is only about 50 days at the minimum by the method of the present invention. The method of the invention realizes the purposes of advancing the flowering phase and shortening the growth phase of the indoor cultivation of industrial hemp. The method has small dependence on the external environment, simple operation, no need of investing a large amount of manpower, low cost of related equipment, capability of meeting the requirement of shortening the breeding period of industrial hemp during actual cultivation and planting, overcoming the restriction problem of illumination and temperature factors during propagation in winter, and extremely high popularization value and economic benefit.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.

Claims (10)

1. A method of promoting premature flowering of industrial hemp, said method comprising: after the industrial hemp is cultured until the second pair of true leaves is unfolded, the industrial hemp is transplanted into a phytotron for culture, and the illumination intensity of a light supplement lamp in the phytotron is adjusted as follows: 30 to 180. mu. mol/m-2·s-1The photoperiod is 12-14 h/10-12 h, and the temperature is 25 +/-1 ℃.
2. The method of claim 1, wherein the fill-in lamp is used to illuminate the substrate at an intensity of 130 μmol-m-2·s-1。
3. The method of claim 1, further comprising: and spraying 200mg/L6-BA + 50-200 mg/L gibberellin to the seedlings of the industrial hemp.
4. The method as claimed in claim 3, wherein 200mg/L6-BA +100mg/L gibberellin is sprayed to seedlings of the industrial hemp.
5. The method as claimed in claim 3, wherein 6-BA + gibberellin is sprayed to the leaf surface every 10 days after the seedlings grow to the fourth pair of true leaves develop.
6. The method of claim 1, wherein the fill light is 50cm from a top-most end of the industrial hemp.
7. The method of claim 1, wherein industrial hemp seeds having a full shape and a uniform size are taken, soaked in clear water for 16-24 hours for germination, then sowed in a nursery tray, watered thoroughly, placed in an artificial cultivation room for seedling cultivation, cultured until a second pair of true leaves is expanded, transplanted in a flowerpot, watered and revived in the dark for one day, and then transplanted in an artificial climate room for cultivation.
8. The method according to claim 7, wherein the light intensity in the artificial culture room is adjusted to 100. mu. mol-m during the raising of seedlings-2·s-1The photoperiod is 16h/8h, and the temperature is 25 ℃.
9. The method as claimed in claim 7, wherein the growth environment is free of plant diseases and insect pests and has sufficient moisture during the seedling raising process.
10. Use of a method of promoting premature flowering of industrial hemp according to any one of claims 1 to 9 for indoor cultivation of industrial hemp.
CN202110467882.2A 2021-04-28 2021-04-28 Method for promoting industrial cannabis to bloom in advance and application thereof Active CN113099971B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110467882.2A CN113099971B (en) 2021-04-28 2021-04-28 Method for promoting industrial cannabis to bloom in advance and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110467882.2A CN113099971B (en) 2021-04-28 2021-04-28 Method for promoting industrial cannabis to bloom in advance and application thereof

Publications (2)

Publication Number Publication Date
CN113099971A true CN113099971A (en) 2021-07-13
CN113099971B CN113099971B (en) 2023-09-08

Family

ID=76720941

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110467882.2A Active CN113099971B (en) 2021-04-28 2021-04-28 Method for promoting industrial cannabis to bloom in advance and application thereof

Country Status (1)

Country Link
CN (1) CN113099971B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113973657A (en) * 2021-11-16 2022-01-28 华中农业大学 Indoor cultivation method of hemp
CN114788479A (en) * 2022-04-24 2022-07-26 浙江省农业科学院 Industrial hemp germplasm resource preservation and breeding method
CN115024166A (en) * 2022-07-08 2022-09-09 云南大学 Method for cultivating full-female seedlings by using male and female heterostrain industrial hemp

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107980494A (en) * 2017-11-29 2018-05-04 黑龙江省科学院大庆分院 A kind of industrial hemp indoor pot breeding method
CN112514741A (en) * 2019-09-19 2021-03-19 黑龙江汉美生工业大麻科技有限公司 Method for controlling flowering time and flowering quantity of industrial hemp

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107980494A (en) * 2017-11-29 2018-05-04 黑龙江省科学院大庆分院 A kind of industrial hemp indoor pot breeding method
CN112514741A (en) * 2019-09-19 2021-03-19 黑龙江汉美生工业大麻科技有限公司 Method for controlling flowering time and flowering quantity of industrial hemp

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
孙宇峰;: "纤维大麻高产栽培技术的研究现状", 中国麻业科学, no. 03, pages 52 - 57 *
辛培尧;何承忠;孙正海;杨明;郭鸿彦;: "短日照处理对大麻开花及性别表达的影响", 湖北农业科学, no. 07, pages 776 - 778 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113973657A (en) * 2021-11-16 2022-01-28 华中农业大学 Indoor cultivation method of hemp
CN114788479A (en) * 2022-04-24 2022-07-26 浙江省农业科学院 Industrial hemp germplasm resource preservation and breeding method
CN115024166A (en) * 2022-07-08 2022-09-09 云南大学 Method for cultivating full-female seedlings by using male and female heterostrain industrial hemp
CN115024166B (en) * 2022-07-08 2023-09-08 云南大学 Method for cultivating full female seedlings of hermaphrodite industrial cannabis sativa

Also Published As

Publication number Publication date
CN113099971B (en) 2023-09-08

Similar Documents

Publication Publication Date Title
CN113099971B (en) Method for promoting industrial cannabis to bloom in advance and application thereof
CN102499090B (en) Method for isolated culture of Haworthia succulent plants
CN102939843B (en) Method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower
CN105075863B (en) A kind of Paeonia papaveracea rapid propagation method
CN103988777B (en) A kind of in-vitro culture method for tender stem segments of the wide yulan of leaflet dwarf form
CN109329029B (en) Ground cover bamboo water culture cultivation method and cultivation device suitable for laboratory research
CN103229701A (en) Cutting propagation method of eustoma grandiflorum
CN104521714A (en) Leaf cuttage rapid propagation method of jonquil
CN109673516A (en) A kind of luminous environment method of the full artificial light plantation bletilla striata
CN111418463A (en) L ED artificial light environment cultivation method suitable for rice whole growth period
CN104920223A (en) Chinese cymbidium seedling breeding method
CN114303820A (en) Method for efficiently producing female hemp seeds in pharmaceutical industry
CN114208618B (en) Method for improving peony propagation efficiency by promoting cultivation in two seasons in one year
CN110786157A (en) Method for promoting rooting of ilex verticillata tissue culture seedlings
CN106718763B (en) Sedum short-shoot cutting cultivation method
CN105494097A (en) In-vitro rapid propagation technology of viburnum sargentii koehne
CN111642351A (en) Method for promoting early flowering of German iris and application
CN109156350B (en) Anti-aleurites fordii propagation bud and rooting culture medium and method for promoting in-vitro rapid propagation of anti-aleurites fordii
CN109247235B (en) Rapid breeding and seedling raising method for cymbidium faberi Rolfe
CN113875590A (en) Rapid cultivation method for clustered North American winter seedlings
CN110731220B (en) Cultivation method for delaying blooming of landscape roses
CN112616601A (en) Indoor planting method for preventing soybeans from climbing tendrils and accelerating soybean growth
CN105660410A (en) Michelia alba tissue culture method
CN105075602A (en) Method for producing seed potatoes
CN115812600B (en) Gyroscope in-vitro rapid propagation technical method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant