CN104904597A - Bletilla striata rapid propagation and sunshine seedling strengthening method - Google Patents

Bletilla striata rapid propagation and sunshine seedling strengthening method Download PDF

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Publication number
CN104904597A
CN104904597A CN201510281953.4A CN201510281953A CN104904597A CN 104904597 A CN104904597 A CN 104904597A CN 201510281953 A CN201510281953 A CN 201510281953A CN 104904597 A CN104904597 A CN 104904597A
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bletilla
seedling
days
propagation
fast
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CN201510281953.4A
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杨宝明
李永平
黄玉玲
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Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
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Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
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Abstract

The present invention relates to a bletilla striata rapid propagation and sunshine seedling strengthening method belonging to the technical field of bletilla striata artificial cultivation. The method comprises the following steps: a, seed induced germination culture, to be more specific, seeds taken from bletilla striata capsules are sowed onto a seed induced germination medium for culturing for 50 days to obtain seedlings; b, rooting culture, to be more specific, the seedlings are put on a rooting medium for culturing for 40 days at the temperature of 23-27 DEG C under the light intensity of 2000-2600Lux to obtain rooting seedlings; and c, sunshine seedling strengthening, to be more specific, the rooting seedlings are transferred to a sunshine greenhouses for culturing for 10 days at the temperature of 20-28 DEG C under the light intensity of 3000-4000Lux, and then the light intensity is adjusted to 5000-6000Lux for culturing for 20 to 25 days. The beneficial effects of the bletilla striata rapid propagation and sunshine seedling strengthening method is that: the bletilla striata rapid propagation and sunshine seedling strengthening method can greatly improve seedling and seed quality, the seedling and seed are thick and solid, and even, the planting survival rate is high, and the survival rate reaches over 95%.

Description

A kind of Fast-propagation of bletilla and sunlight method in strong sprout
Technical field
The invention belongs to bletilla artificial cultivation technique field, be specifically related to a kind of Fast-propagation and sunlight method in strong sprout of bletilla.
Background technology
Bletilla (Bletilla striata (Thunb) Reichb.f) belongs to herbaceos perennial for the orchid family bletilla, widely distributed.The stem tuber of bletilla is China's traditional Chinese medicine, and just on the books in Shennong's Herbal, bletilla pattern is gorgeous in addition, also be a kind of ornamental plants, the demand of market to bletilla expands year by year, and bletilla suffers excessive excavation, cause its wild natural resources sharply to reduce, frequently face extinction.By one of country's Wild Medicinal being classified as focused protection, the wild natural resources of protection bletilla, artificial propagation is imperative with plantation bletilla.The traditional cultivation of bletilla is mainly by division propagation; the division propagation cycle is long, reproductive efficiency is low, consumption kind of amount is large; be difficult to satisfied a large amount of plantation demand; the seed of bletilla is tiny, hypoplasia, without endosperm; be difficult under natural conditions sprout, but bletilla seed is sprouted and is grown comparatively fast on synthetic medium, therefore utilizes plant tissue culture technique can fast breeding bletilla seedling in a large number; to the utilization of bletilla resource, protection and artificial scale, intensive cropping are significant.
Summary of the invention
The object of this invention is to provide a kind of Fast-propagation and sunlight method in strong sprout of bletilla, the method can reduce the cycle of breeding, and pseudobulb is large, and plantation survival rate is high.
The technical solution used in the present invention is a kind of Fast-propagation and sunlight method in strong sprout of bletilla, and the method comprises the following steps:
A, Seed inducement are sprouted and are cultivated: sowed by the seed taken out from bletilla capsule on Seed inducement germination medium, temperature 23 ~ 27 DEG C, cultivate 50 ~ 60 days, obtain seedling under intensity of illumination 2000 ~ 2600Lux condition;
Wherein, 10-15 days before cultivating, seed base portion starts to turn green expanding, and within about 20 days, differentiate spire, within about 50 days, height of seedling degree can reach 2-3cm, and emergence rate reaches 95%, and emerge neat, seedling is sturdy, starts in the stem of seedling the bulb growing 0.1-0.2cm.
Find through Experimental Comparison, intensity of illumination is less than 2000Lux, and speed of emergence is slow, and seedling growth is weak; If intensity of illumination is greater than 2600Lux, seedling growth is slow, easily aging, occurs that top is withered and yellow.
B, culture of rootage: be placed on root media by above-mentioned seedling, temperature 23 ~ 27 DEG C, cultivate 40 days under intensity of illumination 2000 ~ 2600Lux condition, seedling of must taking root;
C, sunlight strong sprout: above-mentioned seedling of taking root is transferred to sunlight booth, temperature 20 ~ 28 DEG C, cultivate 10 ~ 20 days under intensity of illumination 3000 ~ 4000Lux condition, subsequently intensity of illumination is adjusted to 5000 ~ 6000Lux and cultivates 20 ~ 25 days, to obtain final product.
As preferably, in described step a, bletilla capsule was gathered 7 annual ~ September.When gathering, answering that attentional selection pseudobulb is large, plant strain growth is healthy and strong, growing fine, without damage by disease and insect, capsule surface immaculate, small holes caused by worms be explant.
As preferably, in described step a, bletilla capsule is the capsule do not split, and carry out disinfection sterilizing prior to seeding.
As preferably, described sterilization cleans with the surface of suds by bletilla capsule, on aseptic working platform, with alcohol-pickled 30 seconds of 70% ~ 75%, then soak 15 ~ 20 minutes with the mercuric chloride solution of 0.1%, use aseptic water washing again 4 ~ 6 times, finally suck the moisture content on bletilla capsule surface with aseptic filter paper.
As preferably, in described step a, the component of Seed inducement germination medium and content thereof are: 1/2MS+NAA0.1 ~ 0.3mg/L+ sugar 18 ~ 20g/L+ potato 2 ~ 5%+ banana 5 ~ 8%+ agar 4g/L; The pH value 5.6 ~ 6 of this Seed inducement germination medium.
As preferably, the density that in described step a, seed is sowed is each blake bottle sowing 400 ~ 500.
In described step a during the plant height≤2cm of seedling, bud clump proliferated culture medium is adopted to cultivate, component and the content thereof of this bud clump proliferated culture medium are: 1/2MS+6-BA0.5 ~ 0.8mg/L+NAA0.2 ~ 0.3mg/L+ sugar 20 ~ 25g/L+ agar 4g/L+ banana 5 ~ 8%+ potato 2 ~ 5%, pH value 5.6 ~ 6.
As preferably, described bud clump proliferated culture medium is cultivated temperature 23 ~ 27 DEG C, and cultivate under intensity of illumination 2000 ~ 2600Lux condition, light application time is 8 hours/day.
As preferably, when cultivating 50 days in described step a under intensity of illumination 2000 ~ 2600Lux condition, wherein the light application time of first 25 days is 6 hours/day, and the light application time of latter 25 days is 10 hours/day.
As preferably, the plant height > 2cm of seedling in described step b, the component of root media and content thereof are: MS+NAA0.1 ~ 0.2mg/L+IBA0.2 ~ 0.4mg/L+ sugar 30 ~ 35g/L+ agar 4g/L+ banana 5 ~ 8%+ potato 2 ~ 5%, pH value 5.6 ~ 6.
Beneficial effect of the present invention is: sprout through Seed inducement cultivate, the plant height of culture of rootage and sunlight acting in conjunction in strong sprout gained bletilla seedling is 5 ~ 6cm, stem is thick: 0.2 ~ 0.3cm, pseudobulb: 0.5 ~ 0.6cm, Zhan Ye: 6 ~ 7.Blade degree of development is good, roomy abundant.Height of seedling degree is moderate, neatly sturdy; Pseudobulb individuality is comparatively large, and size is even, and domestication survival rate more than 95%, emerges 20 ~ 25 days ahead of time.
Embodiment
For making those skilled in the art understand production technology of the present invention and technique effect in detail, introduce application of the present invention and technique effect further with concrete production instance below.
Embodiment 1:
A, Seed inducement is sprouted and is cultivated: at uncracked capsule of gathering in annual 7-9 month, clean with the surface of suds by bletilla capsule, on aseptic working platform, with alcohol-pickled 30 seconds of 75%, then 18 minutes are soaked with the mercuric chloride solution of 0.1%, use aseptic water washing again 5 times, the moisture content on bletilla capsule surface is finally sucked with aseptic filter paper, capsule two ends are cut with aseptic operation cutter, cut open into two from centre by capsule, the seed taken out from bletilla capsule is sowed on Seed inducement germination medium, temperature 25 DEG C, cultivate 55 days under intensity of illumination 2400Lux condition, wherein the light application time of first 25 days is 6 hours/day, the light application time of latter 30 days is 10 hours/day, obtain seedling, the density that seed is sowed is each blake bottle sowing 400 ~ 500.
B, culture of rootage: be placed on root media by plant height > 2cm seedling, temperature 25 DEG C, cultivate 40 days under intensity of illumination 2300Lux condition, seedling of must taking root; The component of root media and content thereof are: MS+NAA0.15mg/L+IBA0.3mg/L+ sugar 32g/L+ agar 4g/L+ banana 6%+ potato 3%, pH value 5.8.
C, sunlight strong sprout: above-mentioned seedling of taking root is transferred to sunlight booth, temperature 24 DEG C, cultivate 15 days under intensity of illumination 3500Lux condition, subsequently intensity of illumination is adjusted to 5500Lux and cultivates 23 days, to obtain final product.
During the seedling of plant height≤2cm, adopt bud clump proliferated culture medium to cultivate, component and the content thereof of this bud clump proliferated culture medium are: 1/2MS+6-BA0.6mg/L+NAA0.3mg/L+ sugar 23g/L+ agar 4g/L+ banana 6%+ potato 3%, pH value 6.Bud clump proliferated culture medium is cultivated temperature 25 DEG C, and cultivate under intensity of illumination 2300Lux condition, light application time is 8 hours/day.
Embodiment 2:
A, Seed inducement is sprouted and is cultivated: at uncracked capsule of gathering in annual 7-9 month, clean with the surface of suds by bletilla capsule, on aseptic working platform, with alcohol-pickled 30 seconds of 75%, then 20 minutes are soaked with the mercuric chloride solution of 0.1%, use aseptic water washing again 6 times, the moisture content on bletilla capsule surface is finally sucked with aseptic filter paper, capsule two ends are cut with aseptic operation cutter, cut open into two from centre by capsule, the seed taken out from bletilla capsule is sowed on Seed inducement germination medium, temperature 27 DEG C, cultivate 60 days under intensity of illumination 2600Lux condition, wherein the light application time of first 30 days is 6 hours/day, the light application time of latter 30 days is 10 hours/day, obtain seedling, the density that seed is sowed is each blake bottle sowing 400 ~ 500.
B, culture of rootage: be placed on root media by plant height > 2cm seedling, temperature 27 DEG C, cultivate 40 days under intensity of illumination 2600Lux condition, seedling of must taking root; The component of root media and content thereof are: MS+NAA0.2mg/L+IBA0.4mg/L+ sugar 35g/L+ agar 4g/L+ banana 8%+ potato 5%, pH value 6.
C, sunlight strong sprout: above-mentioned seedling of taking root is transferred to sunlight booth, temperature 28 DEG C, cultivate 20 days under intensity of illumination 4000Lux condition, subsequently intensity of illumination is adjusted to 6000Lux and cultivates 25 days, to obtain final product.
During the seedling of plant height≤2cm, adopt bud clump proliferated culture medium to cultivate, component and the content thereof of this bud clump proliferated culture medium are: 1/2MS+6-BA0.8mg/L+NAA0.3mg/L+ sugar 25g/L+ agar 4g/L+ banana 8%+ potato 5%, pH value 5.6 ~ 6.Bud clump proliferated culture medium is cultivated temperature 27 DEG C, and cultivate under intensity of illumination 2600Lux condition, light application time is 8 hours/day.
Embodiment 3:
A, Seed inducement is sprouted and is cultivated: at uncracked capsule of gathering in annual 7-9 month, clean with the surface of suds by bletilla capsule, on aseptic working platform, with alcohol-pickled 30 seconds of 70%, then 15 minutes are soaked with the mercuric chloride solution of 0.1%, use aseptic water washing again 4 times, the moisture content on bletilla capsule surface is finally sucked with aseptic filter paper, capsule two ends are cut with aseptic operation cutter, cut open into two from centre by capsule, the seed taken out from bletilla capsule is sowed on Seed inducement germination medium, temperature 23 DEG C, cultivate 50 days under intensity of illumination 2000Lux condition, wherein the light application time of first 25 days is 6 hours/day, the light application time of latter 25 days is 10 hours/day, obtain seedling, the density that seed is sowed is each blake bottle sowing 400 ~ 500.
B, culture of rootage: be placed on root media by plant height > 2cm seedling, temperature 23 DEG C, cultivate 40 days under intensity of illumination 2000Lux condition, seedling of must taking root; The component of root media and content thereof are: MS+NAA0.1mg/L+IBA0.2mg/L+ sugar 30g/L+ agar 4g/L+ banana 5%+ potato 2%, pH value 5.6.
C, sunlight strong sprout: above-mentioned seedling of taking root is transferred to sunlight booth, temperature 20 DEG C, cultivate 10 days under intensity of illumination 3000Lux condition, subsequently intensity of illumination is adjusted to 5000Lux and cultivates 20 days, to obtain final product.
During the seedling of plant height≤2cm, adopt bud clump proliferated culture medium to cultivate, component and the content thereof of this bud clump proliferated culture medium are: 1/2MS+6-BA0.5mg/L+NAA0.2mg/L+ sugar 20g/L+ agar 4g/L+ banana 5%+ potato 2%, pH value 5.8.Bud clump proliferated culture medium is cultivated temperature 23 DEG C, and cultivate under intensity of illumination 2000Lux condition, light application time is 8 hours/day.
Embodiment 4:
A, Seed inducement is sprouted and is cultivated: at uncracked capsule of gathering in annual 7-9 month, clean with the surface of suds by bletilla capsule, on aseptic working platform, with alcohol-pickled 30 seconds of 75%, then 16 minutes are soaked with the mercuric chloride solution of 0.1%, use aseptic water washing again 5 times, the moisture content on bletilla capsule surface is finally sucked with aseptic filter paper, capsule two ends are cut with aseptic operation cutter, cut open into two from centre by capsule, the seed taken out from bletilla capsule is sowed on Seed inducement germination medium, temperature 24 DEG C, cultivate 58 days under intensity of illumination 2200Lux condition, wherein the light application time of first 29 days is 6 hours/day, the light application time of latter 29 days is 10 hours/day, obtain seedling, the density that seed is sowed is each blake bottle sowing 400 ~ 500.
B, culture of rootage: be placed on root media by plant height > 2cm seedling, temperature 24 DEG C, cultivate 40 days under intensity of illumination 2500Lux condition, seedling of must taking root; The component of root media and content thereof are: MS+NAA0.2mg/L+IBA0.4mg/L+ sugar 30g/L+ agar 4g/L+ banana 5%+ potato 2%, pH value 6.
C, sunlight strong sprout: above-mentioned seedling of taking root is transferred to sunlight booth, temperature 21 DEG C, cultivate 18 days under intensity of illumination 3200Lux condition, subsequently intensity of illumination is adjusted to 5200Lux and cultivates 22 days, to obtain final product.
During the seedling of plant height≤2cm, adopt bud clump proliferated culture medium to cultivate, component and the content thereof of this bud clump proliferated culture medium are: 1/2MS+6-BA0.7mg/L+NAA0.2mg/L+ sugar 24g/L+ agar 4g/L+ banana 8%+ potato 5%, pH value 5.6.Bud clump proliferated culture medium is cultivated temperature 24 DEG C, and cultivate under intensity of illumination 2500Lux condition, light application time is 8 hours/day.
Plant height through above-described embodiment gained bletilla seedling is 5 ~ 6cm, and stem is thick: 0.2 ~ 0.3cm, pseudobulb: 0.5 ~ 0.6cm, Zhan Ye: 6 ~ 7.Blade degree of development is good, roomy abundant.Height of seedling degree is moderate, neatly sturdy; Pseudobulb individuality is comparatively large, and size is even, and domestication survival rate more than 95%, emerges ahead of time 20 ~ 25 days, achieve significant progress.
Finally it should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although with reference to above-described embodiment to invention has been detailed description, those skilled in the art are to be understood that, still can modify to the present invention or equivalent replacement, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in right of the present invention.

Claims (10)

1. the Fast-propagation of bletilla and a sunlight method in strong sprout, is characterized in that: the method comprises the following steps:
A, Seed inducement are sprouted and are cultivated: sowed by the seed taken out from bletilla capsule on Seed inducement germination medium, temperature 23 ~ 27 DEG C, cultivate 50 ~ 60 days, obtain seedling under intensity of illumination 2000 ~ 2600Lux condition;
B, culture of rootage: be placed on root media by above-mentioned seedling, temperature 23 ~ 27 DEG C, cultivate 40 days under intensity of illumination 2000 ~ 2600Lux condition, seedling of must taking root;
C, sunlight strong sprout: above-mentioned seedling of taking root is transferred to sunlight booth, temperature 20 ~ 28 DEG C, cultivate 10 ~ 20 days under intensity of illumination 3000 ~ 4000Lux condition, subsequently intensity of illumination is adjusted to 5000 ~ 6000Lux and cultivates 20 ~ 25 days, to obtain final product.
2. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, is characterized in that: in described step a, bletilla capsule was gathered 7 annual ~ September.
3. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, it is characterized in that: in described step a, bletilla capsule is the capsule do not split, carry out disinfection sterilizing prior to seeding.
4. the Fast-propagation of a kind of bletilla according to claim 3 and sunlight method in strong sprout, it is characterized in that: described sterilization cleans with the surface of suds by bletilla capsule, on aseptic working platform, with alcohol-pickled 30 seconds of 70% ~ 75%, then soak 15 ~ 20 minutes with the mercuric chloride solution of 0.1%, use aseptic water washing again 4 ~ 6 times, finally suck the moisture content on bletilla capsule surface with aseptic filter paper.
5. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, is characterized in that: in described step a, the component of Seed inducement germination medium and content thereof are: 1/2MS+NAA0.1 ~ 0.3mg/L+ sugar 18 ~ 20g/L+ potato 2 ~ 5%+ banana 5 ~ 8%+ agar 4g/L; The pH value 5.6 ~ 6 of this Seed inducement germination medium.
6. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, is characterized in that: the density that in described step a, seed is sowed is each blake bottle sowing 400 ~ 500.
7. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, it is characterized in that: in described step a during the plant height≤2cm of seedling, bud clump proliferated culture medium is adopted to cultivate, component and the content thereof of this bud clump proliferated culture medium are: 1/2MS+6-BA0.5 ~ 0.8mg/L+NAA0.2 ~ 0.3mg/L+ sugar 20 ~ 25g/L+ agar 4g/L+ banana 5 ~ 8%+ potato 2 ~ 5%, pH value 5.6 ~ 6.
8. the Fast-propagation of a kind of bletilla according to claim 7 and sunlight method in strong sprout, is characterized in that: described bud clump proliferated culture medium is cultivated temperature 23 ~ 27 DEG C, and cultivate under intensity of illumination 2000 ~ 2600Lux condition, light application time is 8 hours/day.
9. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, it is characterized in that: when cultivating 50 days in described step a under intensity of illumination 2000 ~ 2600Lux condition, wherein the light application time of first 25 days is 6 hours/day, and the light application time of latter 25 days is 10 hours/day.
10. the Fast-propagation of a kind of bletilla according to claim 1 and sunlight method in strong sprout, it is characterized in that: the plant height > 2cm of seedling in described step b, the component of root media and content thereof are: MS+NAA0.1 ~ 0.2mg/L+IBA0.2 ~ 0.4mg/L+ sugar 30 ~ 35g/L+ agar 4g/L+ banana 5 ~ 8%+ potato 2 ~ 5%, pH value 5.6 ~ 6.
CN201510281953.4A 2015-05-28 2015-05-28 Bletilla striata rapid propagation and sunshine seedling strengthening method Pending CN104904597A (en)

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Cited By (10)

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Publication number Priority date Publication date Assignee Title
CN105766645A (en) * 2016-03-31 2016-07-20 广西医科大学 Efficient reproduction method of rhizoma bletillae tissue culture seedlings
CN106416972A (en) * 2016-08-29 2017-02-22 浙江金石生物科技有限公司 Method for quickly cultivating bletilla striata seedlings
CN106973789A (en) * 2017-03-29 2017-07-25 师宗县福禄农业科技开发有限公司 A kind of root media and its method for culturing seedlings of bletilla striata tissue culture
CN107047310A (en) * 2017-05-10 2017-08-18 文山学院 A kind of cultural method of bletilla striata seeds culture seedling
CN107371681A (en) * 2017-07-05 2017-11-24 佛山杰致信息科技有限公司 A kind of implantation methods of rhizoma Gastrodiae
CN108738473A (en) * 2018-06-06 2018-11-06 武汉叶动力生物科技有限公司 A method of improving bletilla seed germination rate
CN109452171A (en) * 2018-11-26 2019-03-12 丽江海贝瑞生物科技有限公司 A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds
CN110547194A (en) * 2019-09-12 2019-12-10 浙江省东阳玉米研究所 Tissue culture and rapid propagation method for seeds of bletilla striata
CN116649216A (en) * 2023-06-25 2023-08-29 江苏护理职业学院 Pure bletilla striata three-fork tissue culture domesticated seedling raising method
CN116671441A (en) * 2023-07-10 2023-09-01 云南省农业科学院药用植物研究所 Efficient bletilla striata breeding method

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CN104255496A (en) * 2014-09-23 2015-01-07 江苏农林职业技术学院 Method for immediately propagating bletilla striata test-tube plantlet

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105766645A (en) * 2016-03-31 2016-07-20 广西医科大学 Efficient reproduction method of rhizoma bletillae tissue culture seedlings
CN105766645B (en) * 2016-03-31 2018-07-24 广西医科大学 Bletilla tissue-cultured seedling efficient propagation method
CN106416972A (en) * 2016-08-29 2017-02-22 浙江金石生物科技有限公司 Method for quickly cultivating bletilla striata seedlings
CN106973789A (en) * 2017-03-29 2017-07-25 师宗县福禄农业科技开发有限公司 A kind of root media and its method for culturing seedlings of bletilla striata tissue culture
CN107047310A (en) * 2017-05-10 2017-08-18 文山学院 A kind of cultural method of bletilla striata seeds culture seedling
CN107371681A (en) * 2017-07-05 2017-11-24 佛山杰致信息科技有限公司 A kind of implantation methods of rhizoma Gastrodiae
CN108738473A (en) * 2018-06-06 2018-11-06 武汉叶动力生物科技有限公司 A method of improving bletilla seed germination rate
CN109452171A (en) * 2018-11-26 2019-03-12 丽江海贝瑞生物科技有限公司 A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds
CN110547194A (en) * 2019-09-12 2019-12-10 浙江省东阳玉米研究所 Tissue culture and rapid propagation method for seeds of bletilla striata
CN116649216A (en) * 2023-06-25 2023-08-29 江苏护理职业学院 Pure bletilla striata three-fork tissue culture domesticated seedling raising method
CN116671441A (en) * 2023-07-10 2023-09-01 云南省农业科学院药用植物研究所 Efficient bletilla striata breeding method

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