CN107371681A - A kind of implantation methods of rhizoma Gastrodiae - Google Patents
A kind of implantation methods of rhizoma Gastrodiae Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention belongs to field of agricultural cultivation, and in particular to a kind of implantation methods of rhizoma Gastrodiae, comprise the following steps：S1, selection of land site preparation；S2, rhizoma Gastrodiae nursery；S3, transplanting；S4, the prevention and control of plant diseases, pest control.The present invention improves the speed of growth of rhizoma Gastrodiae, reduces the incidence of pest and disease damage, especially to reducing southern blight, black rot and the incidence significant effect of mealybug.
Description
Technical field
The invention belongs to field of agricultural cultivation, and in particular to a kind of implantation methods of rhizoma Gastrodiae.
Background technology
Rhizoma Gastrodiae (Gastrodia elata BI.), also known as rhizoma gastrodiae, solely shake sesame, from it is female, close from grass, refreshing grass, yellow moccasin flower, wood
Pu, RHIZONA GASTRODIAE, DINGFENGCAO, Bai Longpi etc., it is orchid family Gastrodia herbaceos perennial.Root-like stock is plump, and no greenery, capsule falls
Ovate-elliptic, often with stem tuber or seminal propagation.Its rhizome be used as medicine to treat have a dizzy spell, extremity numbness, child convulsion etc.
Disease, it is rare Chinese medicine, treatment headache and insomnia is shared with fine jade treasure ganoderma lucidum.
Authorized announcement date is August in 2015 12, and Authorization Notice No. is CN103650880B Chinese invention patent, open
A kind of rhizoma Gastrodiae plantation cultural method, is brewed into pastel using by corn flour, mix with active powdered carbon, it is beautiful that pasty state is made
Rice and Mixture of Activated Carbon, the mixture is evenly laid out in square position, and it is in gel to treat the mixture, bacteria stick, fiber crops
Kind is planted again after closely rolling.The invention improves seed germination rate and connects bacterium rate, but its prevention and control of plant diseases, pest control is unfavorable.
Data of publication of application is on November 9th, 2016, and application publication number is CN106069043A Chinese invention patent application,
A kind of rhizoma Gastrodiae implantation methods are disclosed, sawdust, rice bran, straw, WUDUOYUN powder, Bright Yellow powder, dandelion is chosen and circle is made
Column, rolled one week in halimasch kind, obtain bacteria stick, chosen without mildew and rot broad-leaved leaf, broad-leaved leaf is laid on level land,
And 0.2-0.7kg seed dressing is spread, wherein, seed dressing is the mixture of Germination Strain and rhizoma Gastrodiae capsule；Bacteria stick is placed in broad-leaved leaf
One end, and rolled, obtain broad-leaved volume rod；Take rice, after being ground into powder, with water according to mass ratio be 1:3-5 is mixed
Conjunction is brewed into pastel, and the nano-carbon powder for accounting for pastel quality 3-7% is added to pasty state species, stirs, and put down
It is layered on square position, after it is gelatinous mixture, broad-leaved volume rod is placed in one end of gelatinous mixture, rolls, is rolled up
Rod；Use river sand to spread a layer thickness as 2-3cm, volume rod is laid in above, then 1-3cm is covered using fertile soil, then using river
Sand covering 2-3cm, according to Routine Management, you can.The invention can improve the contact rate of halimasch, but the growth of rhizoma Gastrodiae is relatively slow
Slowly, pest and disease damage incidence is higher.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of implantation methods of rhizoma Gastrodiae, to accelerate the growth of rhizoma Gastrodiae and drop
The pest and disease damage incidence of low rhizoma Gastrodiae.
In order to solve the above-mentioned technical problem, the technical scheme is that：
A kind of implantation methods of rhizoma Gastrodiae, comprise the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 30~50cm,
Every mu is applied 150~300kg of quick lime, 200~300kg of plant ash, ploughs deeply 30~50cm, pours permeable, solarization 10~20 days, is excavated wide
The kind planting ditch for being 20~30cm for 1~1.2m, depth, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, seeds of Gastrodia elata is placed in 0.1~0.5% (m/v)
5~10s in mercuric chloride solution, then seeds of Gastrodia elata is placed in 3~5s in 75% (v/v) ethanol, sterile water wash 2~5 times, by day
Numb seed is homogeneously disposed on Seed inducement germination medium, is placed in 10~25 DEG C of illumination box and is cultivated, and daily illumination 7~
12 hours, when rhizoma Gastrodiae seedling length is to 2~3cm, be inoculated with into Seed inducement germination medium into halimasch, rhizoma Gastrodiae seedling grow to
During 4~5cm, transplanted；
S3, transplanting：The corncob cellulose that one thickness of paving is 10~20cm in ready kind of planting ditch in step sl, repaves one
5~8cm of layer sawdust, halimasch is sprinkled on sawdust, rhizoma Gastrodiae seedling is transplanted into sawdust by 10~20 × 10~20cm,
The soil that thickness is 2~3cm is backfilled on sawdust, in one layer of soil upper berth sweet wormwood, is poured permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution；
In the implantation methods of rhizoma Gastrodiae provided by the invention, it is preferable that the Seed inducement germination medium is：1/2MS+
6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharide 0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.0~
6.5。
In the implantation methods of rhizoma Gastrodiae provided by the invention, it is preferable that the inoculum concentration of halimasch described in step S2 is kind
0.5~the 3 ‰ of son induction germination medium weight.
In the implantation methods of rhizoma Gastrodiae provided by the invention, it is preferable that the trichoderma harzianum with bacterium be 130~
220g/ mus.
In the implantation methods of rhizoma Gastrodiae provided by the invention, it is preferable that the Chinese medicine extract includes following parts by weight
Raw material forms：30~70 parts of Verbena officinalis, 20~50 parts of monkshood, 23~47 parts of the fruit of glossy privet, 26~63 parts of the tuber of pinellia, pipewort 13~28
3~10 parts of part, 10~25 parts of thick wood-fern rhizome and chinaroot greenbrier.
In the implantation methods of rhizoma Gastrodiae provided by the invention, it is preferable that the Chinese medicine extract includes following parts by weight
Raw material forms：55 parts of Verbena officinalis, 39 parts of monkshood, 25 parts of the fruit of glossy privet, 46 parts of the tuber of pinellia, 20 parts of pipewort, 17 parts of thick wood-fern rhizome and greenbrier
6 parts of chinaroot greenbrier.
In the implantation methods of rhizoma Gastrodiae provided by the invention, it is preferable that the preparation method of the Chinese medicine extract is included such as
Lower step：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, 10~50 mesh are crushed to, by material
Liquid weight is than 1:5~8 add water to cook 2~5 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:3~5 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
Using above-mentioned technical proposal, because the mode using tissue cultures carries out nursery to rhizoma Gastrodiae, seeds of Gastrodia elata is promoted
Germination percentage；When in tissue cultures, rhizoma Gastrodiae seedling length is to 2~3cm, it is inoculated with into Seed inducement germination medium into halimasch,
Accelerate the combination of halimasch and rhizoma Gastrodiae, improve the growth rate of rhizoma Gastrodiae；Spray Chinese medical extract and trichoderma harzianum bacterium solution is applied in filling
There is synergy synergy to reducing southern blight and black rot incidence；Spray Chinese medical extract has to reducing mealybug incidence
Remarkable result.General planting rhizoma Gastrodiae is, it is necessary to which 3 years could harvest, and use implantation methods provided by the invention, after rhizoma Gastrodiae transplanting
1 year can harvest, and tissue cultivating and seedling is not by season and climatic effect.The present invention improves the speed of growth of rhizoma Gastrodiae,
Reduce the incidence of southern blight, black rot and mealybug.
Embodiment
The embodiment of the present invention is described further below.Herein it should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but does not form limitation of the invention.In addition, invention described below
As long as involved technical characteristic does not form conflict can and is mutually combined each other in each embodiment.
Embodiment 1
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 50cm, every mu
Quick lime 300kg, plant ash 200kg are applied, ploughs deeply 50cm, pours permeable, solarization 20 days, excavates a width of 1~1.2m, the kind that depth is 30cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.1% (m/v) mercuric chloride
10s in liquid, then seeds of Gastrodia elata is placed in 5s in 75% (v/v) ethanol, sterile water wash 2 times, seeds of Gastrodia elata is homogeneously disposed in
On Seed inducement germination medium, be placed in 25 DEG C of illumination box and cultivate, daily illumination 12 hours, rhizoma Gastrodiae seedling grow to
During 2~3cm, it is inoculated with into Seed inducement germination medium by inoculum concentration for 0.5 ‰ into halimasch, rhizoma Gastrodiae seedling length to 4~5cm
When, transplanted；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ shells
Oligosaccharides 0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.0.
S3, transplanting：The corncob cellulose that one thickness of paving is 10cm in ready kind of planting ditch in step sl, repaves one layer
8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 20 × 10cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
Chinese medicine extract forms including following parts by weight raw material：70 parts of Verbena officinalis, 20 parts of monkshood, 23 parts of the fruit of glossy privet, 63 parts of the tuber of pinellia, paddy
Smart 13 parts of grass, 25 parts of thick wood-fern rhizome and chinaroot greenbrier 3.
The preparation method of Chinese medicine extract comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 10 mesh, by feed liquid weight
Measure ratio 1:5 add water to cook 2 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:5 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution, trichoderma harzianum
It is 130g/ mus with bacterium.
Embodiment 2
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 50cm, every mu
Quick lime 150kg, plant ash 300kg are applied, ploughs deeply 30cm, pours permeable, solarization 10 days, excavates a width of 1~1.2m, the kind that depth is 20cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.5% (m/v) mercuric chloride
5s in liquid, then seeds of Gastrodia elata is placed in 3s in 75% (v/v) ethanol, sterile water wash 5 times, seeds of Gastrodia elata is homogeneously disposed in kind
Son induction germination medium on, be placed in 10 DEG C of illumination box and cultivate, daily illumination 7 hours, rhizoma Gastrodiae seedling length to 2~
During 3cm, it is inoculated with into halimasch for 3 ‰ by inoculum concentration into Seed inducement germination medium, when rhizoma Gastrodiae seedling length is to 4~5cm, enters
Row transplanting；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharides
0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.5.
S3, transplanting：The corncob cellulose that one thickness of paving is 20cm in ready kind of planting ditch in step sl, repaves one layer
5cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 20 × 20cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
Chinese medicine extract forms including following parts by weight raw material：30 parts of Verbena officinalis, 50 parts of monkshood, 47 parts of the fruit of glossy privet, 26 parts of the tuber of pinellia, paddy
Smart 28 parts of grass, 10 parts of thick wood-fern rhizome and chinaroot greenbrier 10.
The preparation method of Chinese medicine extract comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 50 mesh, by feed liquid weight
Measure ratio 1:8 add water to cook 5 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:3 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution, trichoderma harzianum
It is 220g/ mus with bacterium.
Embodiment 3
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 50cm, every mu
Quick lime 200kg, plant ash 260kg are applied, ploughs deeply 40cm, pours permeable, solarization 15 days, excavates a width of 1~1.2m, the kind that depth is 25cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.3% (m/v) mercuric chloride
8s in liquid, then seeds of Gastrodia elata is placed in 4s in 75% (v/v) ethanol, sterile water wash 3 times, seeds of Gastrodia elata is homogeneously disposed in kind
On son induction germination medium, it is placed in 15 DEG C of illumination box and cultivates, daily illumination 10 hours, in rhizoma Gastrodiae seedling length to 2
By inoculum concentration it is 1 ‰ to be inoculated with into halimasch into Seed inducement germination medium during~3cm, when rhizoma Gastrodiae seedling length is to 4~5cm,
Transplanted；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharides
0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.2.
S3, transplanting：The corncob cellulose that one thickness of paving is 15cm in ready kind of planting ditch in step sl, repaves one layer
8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 10 × 10cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
Chinese medicine extract forms including following parts by weight raw material：55 parts of Verbena officinalis, 39 parts of monkshood, 25 parts of the fruit of glossy privet, 46 parts of the tuber of pinellia, paddy
Smart 20 parts of grass, 17 parts of thick wood-fern rhizome and chinaroot greenbrier 6.
The preparation method of Chinese medicine extract comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 30 mesh, by feed liquid weight
Measure ratio 1:7 add water to cook 4 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:4 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution, trichoderma harzianum
It is 180g/ mus with bacterium.
Embodiment 4
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 40cm, every mu
Quick lime 230kg, plant ash 280kg are applied, ploughs deeply 40cm, pours permeable, solarization 18 days, excavates a width of 1~1.2m, the kind that depth is 28cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.2% (m/v) mercuric chloride
6s in liquid, then seeds of Gastrodia elata is placed in 4s in 75% (v/v) ethanol, sterile water wash 2 times, seeds of Gastrodia elata is homogeneously disposed in kind
On son induction germination medium, it is placed in 18 DEG C of illumination box and cultivates, daily illumination 11 hours, in rhizoma Gastrodiae seedling length to 2
By inoculum concentration it is 2 ‰ to be inoculated with into halimasch into Seed inducement germination medium during~3cm, when rhizoma Gastrodiae seedling length is to 4~5cm,
Transplanted；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharides
0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.4.
S3, transplanting：The corncob cellulose that one thickness of paving is 15cm in ready kind of planting ditch in step sl, repaves one layer
8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 10 × 10cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
Chinese medicine extract forms including following parts by weight raw material：50 parts of Verbena officinalis, 30 parts of monkshood, 35 parts of the fruit of glossy privet, 56 parts of the tuber of pinellia, paddy
Smart 25 parts of grass, 23 parts of thick wood-fern rhizome and chinaroot greenbrier 7.
The preparation method of Chinese medicine extract comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 40 mesh, by feed liquid weight
Measure ratio 1:7 add water to cook 4 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:5 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution, trichoderma harzianum
It is 190g/ mus with bacterium.
Embodiment 5
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 40cm, every mu
Quick lime 260kg, plant ash 230kg are applied, ploughs deeply 45cm, pours permeable, solarization 14 days, excavates a width of 1~1.2m, the kind that depth is 28cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.4% (m/v) mercuric chloride
6s in liquid, then seeds of Gastrodia elata is placed in 4s in 75% (v/v) ethanol, sterile water wash 3 times, seeds of Gastrodia elata is homogeneously disposed in kind
On son induction germination medium, it is placed in 20 DEG C of illumination box and cultivates, daily illumination 12 hours, rhizoma Gastrodiae seedling length to 4~
During 5cm, transplanted；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA0.2mg/L+LFS 0.6mg/L+
Chitosan oligosaccharide 0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH6.0.
S3, transplanting：The corncob cellulose that one thickness of paving is 15cm in ready kind of planting ditch in step sl, repaves one layer
8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 10 × 10cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
Chinese medicine extract forms including following parts by weight raw material：40 parts of Verbena officinalis, 39 parts of monkshood, 35 parts of the fruit of glossy privet, 50 parts of the tuber of pinellia, paddy
Smart 25 parts of grass, 23 parts of thick wood-fern rhizome and chinaroot greenbrier 8.
The preparation method of Chinese medicine extract comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 40 mesh, by feed liquid weight
Measure ratio 1:7 add water to cook 4 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:5 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution, trichoderma harzianum
It is 160g/ mus with bacterium.
Embodiment 6
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 40cm, every mu
Quick lime 230kg, plant ash 280kg are applied, ploughs deeply 40cm, pours permeable, solarization 18 days, excavates a width of 1~1.2m, the kind that depth is 28cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.2% (m/v) mercuric chloride
6s in liquid, then seeds of Gastrodia elata is placed in 4s in 75% (v/v) ethanol, sterile water wash 2 times, seeds of Gastrodia elata is homogeneously disposed in kind
On son induction germination medium, it is placed in 18 DEG C of illumination box and cultivates, daily illumination 11 hours, in rhizoma Gastrodiae seedling length to 2
By inoculum concentration it is 2 ‰ to be inoculated with into halimasch into Seed inducement germination medium during~3cm, when rhizoma Gastrodiae seedling length is to 4~5cm,
Transplanted；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharides
0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.4.
S3, transplanting：The corncob cellulose that one thickness of paving is 15cm in ready kind of planting ditch in step sl, repaves one layer
8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 10 × 10cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution, trichoderma harzianum
It is 190g/ mus with bacterium.
Embodiment 7
A kind of implantation methods of rhizoma Gastrodiae are present embodiments provided, are comprised the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 40cm, every mu
Quick lime 200kg, plant ash 280kg are applied, ploughs deeply 40cm, pours permeable, solarization 15 days, excavates a width of 1~1.2m, the kind that depth is 28cm
Planting ditch, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, it is molten that seeds of Gastrodia elata is placed in into 0.3% (m/v) mercuric chloride
8s in liquid, then seeds of Gastrodia elata is placed in 4s in 75% (v/v) ethanol, sterile water wash 3 times, seeds of Gastrodia elata is homogeneously disposed in kind
On son induction germination medium, it is placed in 18 DEG C of illumination box and cultivates, daily illumination 11 hours, in rhizoma Gastrodiae seedling length to 2
By inoculum concentration it is 2 ‰ to be inoculated with into halimasch into Seed inducement germination medium during~3cm, when rhizoma Gastrodiae seedling length is to 4~5cm,
Transplanted；Seed inducement germination medium is：1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharides
0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH 6.4.
S3, transplanting：The corncob cellulose that one thickness of paving is 20cm in ready kind of planting ditch in step sl, repaves one layer
8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 10 × 10cm, is backfilled on sawdust thick
For 2~3cm soil, in one layer of soil upper berth sweet wormwood, pour permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
Chinese medicine extract forms including following parts by weight raw material：65 parts of Verbena officinalis, 42 parts of monkshood, 35 parts of the fruit of glossy privet, 53 parts of the tuber of pinellia, paddy
Smart 25 parts of grass, 20 parts of thick wood-fern rhizome and chinaroot greenbrier 5.
The preparation method of Chinese medicine extract comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 40 mesh, by feed liquid weight
Measure ratio 1:6 add water to cook 4 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:5 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
Test example
In order to verify the technique effect of the present invention, rhizoma Gastrodiae plantation is carried out by the method for embodiment 1 to 7 respectively, to rhizoma Gastrodiae disease
Insect pest incidence is counted, and the results are shown in Table 1.
The rhizoma Gastrodiae pest and disease damage incidence statistical result of table 1.
| Group | Southern blight incidence | Black rot incidence | Mealybug incidence |
| Embodiment 1 | 1.1 | 0 | 3 |
| Embodiment 2 | 1.3 | 0.5 | 4 |
| Embodiment 3 | 0 | 0 | 1 |
| Embodiment 4 | 1.0 | 0 | 0 |
| Embodiment 5 | 1.2 | 0 | 1 |
| Embodiment 6 | 2.2 | 3.5 | 8 |
| Embodiment 7 | 2.5 | 5 | 2 |
As can be seen from the above table, the incidence embodiment 6 and 7 of southern blight and black rot is significantly higher than embodiment 1 to 5, powder
A red-spotted lizard incidence embodiment 6 is significantly higher than embodiment 1 to 5 and 7.Chinese medicine is not sprayed in the implantation methods of embodiment 5 to rhizoma Gastrodiae to carry
Thing is taken, does not have filling to apply trichoderma harzianum bacterium solution in the implantation methods of embodiment 6.It can be seen that spray Chinese medical extract and fill Shi Haci
Trichoderma bacterium solution has synergy synergy to reducing southern blight and black rot incidence；Chinese medical extract is sprayed to reducing mealybug
Incidence has remarkable result.
Embodiments of the present invention are explained in detail above, but the invention is not restricted to described embodiment.It is right
For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more
Kind change, modification, replacement and modification, still fall within protection scope of the present invention.
Claims (7)
1. a kind of implantation methods of rhizoma Gastrodiae, it is characterised in that comprise the following steps：
S1, selection of land site preparation：Chiltern or sandy loam field of the height above sea level for 800~1200m areas are selected, deep plough 30~50cm, every mu
150~300kg of quick lime, 200~300kg of plant ash are applied, ploughs deeply 30~50cm, pours permeable, solarization 10~20 days, excavation a width of 1
~1.2m, the kind planting ditch that depth is 20~30cm, it is standby；
S2, rhizoma Gastrodiae nursery：Rhizoma Gastrodiae capsule is aseptically crumbed, seeds of Gastrodia elata is placed in 0.1~0.5% (m/v) mercuric chloride
5~10s in solution, then seeds of Gastrodia elata is placed in 3~5s in 75% (v/v) ethanol, sterile water wash 2~5 times, by Gastrodia
Son is homogeneously disposed on Seed inducement germination medium, is placed in 10~25 DEG C of illumination box and is cultivated, and daily illumination 7~12 is small
When, when rhizoma Gastrodiae seedling length is to 2~3cm, it is inoculated with into Seed inducement germination medium into halimasch, rhizoma Gastrodiae seedling length to 4~
During 5cm, transplanted；
S3, transplanting：The corncob cellulose that one thickness of paving is 10~20cm in ready kind of planting ditch in step sl, repaves one layer 5
~8cm sawdust, is sprinkled into halimasch on sawdust, and rhizoma Gastrodiae seedling is transplanted into sawdust by 10~20 × 10~20cm, sawed
The soil that thickness is 2~3cm is backfilled on end, in one layer of soil upper berth sweet wormwood, is poured permeable；
S4, the prevention and control of plant diseases, pest control：
Chinese medicine extract is sprayed for the first time immediately after rhizoma Gastrodiae transplanting, and Chinese medicine extract is sprayed for the second time in rhizoma Gastrodiae Post flowering；
When rhizoma Gastrodiae length is to a height of 20~30cm, is filled to Gastrodia Elata Stem Tuber portion and apply trichoderma harzianum bacterium solution.
2. the implantation methods of rhizoma Gastrodiae according to claim 1, it is characterised in that the Seed inducement germination medium is：
1/2MS+6-BA 2mg/L+NAA 0.2mg/L+LFS 0.6mg/L+ chitosan oligosaccharide 0.4mg/L+ sucrose 30mg/L+ agar 8g/L, pH
6.0~6.5.
3. the implantation methods of rhizoma Gastrodiae according to claim 1, it is characterised in that the inoculum concentration of halimasch described in step S2
For 0.5~the 3 ‰ of Seed inducement germination medium weight.
4. the implantation methods of rhizoma Gastrodiae according to claim 1, it is characterised in that the trichoderma harzianum is 130 with bacterium
~220g/ mus.
5. the implantation methods of rhizoma Gastrodiae according to claim 1, it is characterised in that the Chinese medicine extract includes following weight
Number raw material forms：30~70 parts of Verbena officinalis, 20~50 parts of monkshood, 23~47 parts of the fruit of glossy privet, 26~63 parts of the tuber of pinellia, pipewort 13
3~10 parts of~28 parts, 10~25 parts of thick wood-fern rhizome and chinaroot greenbrier.
6. the implantation methods of rhizoma Gastrodiae according to claim 5, it is characterised in that the Chinese medicine extract includes following weight
Number raw material forms：55 parts of Verbena officinalis, 39 parts of monkshood, 25 parts of the fruit of glossy privet, 46 parts of the tuber of pinellia, 20 parts of pipewort, 17 parts of thick wood-fern rhizome
With 6 parts of chinaroot greenbrier.
7. the implantation methods of the rhizoma Gastrodiae according to claim 5 or 6, it is characterised in that the preparation side of the Chinese medicine extract
Method comprises the following steps：
S1, Verbena officinalis, the fruit of glossy privet, pipewort, thick wood-fern rhizome and chinaroot greenbrier are weighed by proportioning, be crushed to 10~50 mesh, by feed liquid weight
Measure ratio 1:5~8 add water to cook 2~5 hours, filtering, obtain filtrate；
S2, by proportioning monkshood and the tuber of pinellia are weighed, smashed to pieces, by solid-liquid ratio 1:3~5 add water stirring, filtering, obtain filtrate；
S3, the filtrate described in step S1 and S2 merged, obtain the Chinese medicine extract.
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| CN109362518A (en) * | 2018-11-05 | 2019-02-22 | 黎平青江农业开发有限公司 | A kind of method for culturing seedlings of Rhizoma Gastrodiae |
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