CN102823503B - Tissue culture medium for propagating anthurium buds by using buds - Google Patents
Tissue culture medium for propagating anthurium buds by using buds Download PDFInfo
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Abstract
The invention discloses a tissue culture medium for propagating anthurium buds by using buds. The culture medium comprises a subculture medium and a rooting medium. The tissue culture medium adopts direct induction of germ-free buds and proliferation and propagation instead of callus dedifferentiation for the first time. Compared with the callus dedifferentiation, the technique can be used for fast and easily obtaining germ-free buds, can avoid variation occurred during subculture proliferation, guarantees the grade and quality of seedlings, and is beneficial to storage and production utilization of germplasm resources.
Description
Technical field
The present invention relates to tissue culture medium (TCM), relate in particular to a kind of red palm with the numerous bud culture medium for tissue culture of bud.
Background technology
The red palm (anthurium) has another name called that the peace ancestral spends, fiery crane flower etc., belongs to the Araeceae Anthurium, originates in the tropical rain forest area of South America, and all there is extensive cultivation in existing Europe, Asia, Africa.Characteristic is the warm chilly of happiness, the wet fearness drought of happiness, and happiness the moon is avoided solarization.Its flower uniqueness has spadix, and is bright in colour magnificent, rich color, and leaf bract, common bract color has redness, pink, white etc., and great ornamental value is arranged.The breeding of methods such as available sowing, plant division.The flower language of the red palm be ride on the crest of success, enthusiasm, warm blood.
The red palm is important tropical cut-flower, spadix, and its Buddhist flame petal is very large, plump tool wax, that color and luster has is red, powder, white, green, double-colored etc.Its lovely luster, appearance is peculiar, applied range, the economic worth height is high-grade tropical cut-flower and the pot flowers that present global evolution is fast, demand is bigger.Red palm country of origin tropical rain forest, the available seminal propagation of the red palm, but it is long to enter flowering time.Division propagation is the red palm main mode of breeding in the past.Red palm plant base portion grows the suction bud, but plant division behind the generation root system, can divide the 3-4 strain every year, and breeding coefficient is lower, is difficult to satisfy the required seedling of large-scale production.The quick breeding of seedling, the just clone technology of the red palm are mainly carried out in the production of present red palm seedling by tissue culture.Like this can be in the relatively short time high quality seedling of production neat and consistent, the needs that supply is produced.By tissue culture technique sport technique segment such as produce foundation, multiplication culture, strengthening seedling and rooting that red palm seedling mainly contains regeneration system, transplant and nurse young plants in hothouses.
Domestic and international research report to red palm tissue culture all is to carry out callus induction with organs such as blade, petiole, stem sections at present, and then evoked callus differentiation indefinite bud, variation appears in indefinite bud easily that obtain by this approach in breeding, influenced the original good inherited character of seedling.And be directly to induce stem section differentiation lateral bud in the mode of the numerous bud of bud, induce lateral bud differentiation indefinite bud to breed again, the indefinite bud that obtains by this approach can effectively be avoided the variation that occurs in the shoot proliferation process, keeps the original good character of maternal plant better.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide a kind of red palm with the numerous bud culture medium for tissue culture of bud, this substratum comprises subculture medium and root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1800mg/L, ammonium nitrate 1350mg/L, Calcium dichloride dihydrate 350mg/L, magnesium sulfate heptahydrate 320mg/L, potassium primary phosphate 150mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, riboflavin (VB
2) 6.0mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.5-2.0mg/L, naphthylacetic acid 0.2-0.8mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 900mg/L, ammonium nitrate 675mg/L, Calcium dichloride dihydrate 175mg/L, magnesium sulfate heptahydrate 160mg/L, potassium primary phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.3-0.6mg/L;
Surplus is distilled water.
The red palm of the present invention with the subculture in the numerous bud culture medium for tissue culture of bud, the compound method of root media is:
The preparation of 1 mother liquor and preservation
1.1 for ease of sampling, the various mother liquors of suitable preparation earlier are divided into macroelement mother liquor, micro-mother liquor, organism mother liquor and each plant growth regulators mother liquor.Sucrose, agar should not be made into mother liquor, directly claim sample when needing;
1.2 the macroelement mother liquid concentration becomes 100 times of solution, organism, micro-mother liquor are made into 200 times of solution, and the concentration of plant growth regulating reagent mother liquor is made into 1mg/ml;
1.3 mother liquor is selected aseptic distilled water, deionized water or ultrapure water preparation for use, uses the water that boiled during mass production;
1.4 during preparation macroelement mother liquor, each component should be dissolved separately, mix one by one by the order of nitrogen, calcium, magnesium, phosphorus the back, otherwise cause precipitation easily;
1.5 trace element, organism, plant-growth regulator mother liquor are used brown bottle and are installed and place refrigerator to preserve;
1.6 should in time use after the mother liquor preparation, period of storage should not be above 1 month;
1.7 find that mother liquor has precipitation, or microorganism growth arranged, or algal grown is arranged, should pass into disuse.
2 culture medium preparation
2.1 according to culture medium prescription, measure various mother liquors in proportion;
2.2 in dosing container, put into about pure water more than 1/2 of preparing preparation substratum total amount, and add an amount of sugar, add the mother liquor of aequum then while stirring one by one;
But 2.3 add after the agar heat fused, also can directly add agar powder during if any whipping device, supply the total amount of required preparation substratum then with pure water, stirring gets final product;
2.4 the sugar in the substratum, in a large amount of tissue cultured seedling are produced, general available commercially available white sugar, but preferably do not want garden beet sugar with sucrose;
2.5 regulate the pH value to 5.8 of substratum with the sodium hydroxide of the hydrochloric acid of 1.0 mol or 1.0 mol;
2.6 will dividing as early as possible, prepared culture medium installs in the culture vessel, in order to avoid culture medium solidifying or retrogradation and be difficult to packing;
2.7 should note during the packing substratum avoiding substratum to be bonded on the bottleneck, if be stained with substratum, before lid bottle cap or bottle stopper, must clean bottleneck with clean gauze.
3 substratum sterilizations
3.1 being loaded in the high-pressure sterilizing pot, the substratum that branch is installed sterilizes;
3.2 the heating initial stage when the air pressure of sterilizing pan sterilized room reaches 0.05Mpa, is opened condensation trap, drains freezing air in the sterilized room;
3.3 when the sterilized room internal gas pressure reaches 0.11Mpa, when temperature reaches 121 ℃, picks up counting, keep temperature and pressure sterilization 15-20 minute;
3.4 close the heating power supply switch, by slow exhaust mode discharging hot gas, when being down to normal atmosphere, opens indoor air pressure to be sterilized sterilization pot cover or door, take out substratum;
3.5 substratum should place little air to flow and the clean environment cooling of few dust, otherwise causes mould spores to enter the cultivation vessel in the cooling intake process, produces mould contamination.
4 substratum storage
4.1 substratum should be now with the current as far as possible;
4.2 can be at air clean and immobilising environment short period of time storage substratum, should pass into disuse but storage surpasses 1 month substratum.
The red palm of the present invention with the method for the numerous bud tissue culture of bud is:
1. bud induces
In the tender stem of the red palm of fine clip robust growth, no disease and pest as explant, cut off blade, clean 1-2 time with the water that is added with a little liquid detergent, and scrub internode gently with banister brush, rinse well with flowing water again, then on Bechtop with the stem section with 75% alcohol disinfecting 30 seconds, aseptic water washing 3 times, again with 0.1% mercuric chloride sterilization 10min, aseptic water washing 5～6 times.The material that has disinfected is cut into the sections that has 1-2 lateral bud, and base portion is inoculated in downwards in the substratum of induced bud then.When explant is cultivated for the first time, need secretly cultivate about 10 days entirely, be put under the low light level again and cultivate, about cultivation stem section through about 20 days can induce sprouting, and then moves to the place cultivation of seeing light.
2. the multiplication culture of bud
The sprouting of inducing acquisition is seeded to induces the differentiation bud of growing thickly to carry out the shoot proliferation of bud on the subculture medium, general 40 days subcultures once, the subculture material all is placed on the cultivation indoor cultivation, culturing room's temperature is (25 ± 2) ℃, illumination 12h.d
-1, illuminance 1500lx.
3. what take root induces
When budling grows to moon 2.0cm, will go to root induction on the root media by single cutting-out on the bud of growing thickly than healthy and strong bud seedling, all the other bud seedlings go to and proceed multiplication culture on the subculture medium.Root culture need be cultivated under the low light level about 10 days, and this moment, the base portion of seedling formed white projection, and elongation gradually, can form obvious young root after 15 days, grew up to complete plantlet gradually, should progressively increase illumination this period and make seedling become sturdy.
4. the take root transplanting of seedling
The abundant lignifying of seedling of waiting to take root, blade is unfolded, and the leaf look dark green, and the caulom elongation can be carried out the booth transplanting during the about 3.0cm of height of seedling.Take out seedling during transplanting earlier, after cleaning the substratum of base portion, transplant on the usefulness of sterilize through 0.1% potassium permanganate is grown seedlings the good seedling medium of support the about 100-120 strain seedling of every holder transplanting, irrigate normal root water, keep certain temperature and humidity, temperature is controlled at 25 ℃ ± 5 ℃, humidity about 70%, matrix see dried see wet for well, too wet meeting causes mashed root, and it is withered that blade appears in too dried meeting, and will regularly spray medicine and carry out diseases prevention and handle.
Advantage of the present invention is:
1, adopts first without the callus dedifferentiation, directly induce aseptic bud and expand numerous propagation with the numerous bud of bud, this technology is faster, easier than obtain aseptic bud material by callus, and can effectively avoid the variation that occurs in the shoot proliferation process, guaranteed quality and the quality of seedling, be conducive to germ plasm resource and preserve and produce and utilize;
2, carry out the red palm with the numerous bud multiplication culture of bud with subculture medium, summer, culture temperature remained on 25-28 ℃, and winter, culture temperature kept about 20 ℃, and the subculture seedling moon is bred 3.8 times, and a year breeding coefficient is 3.8
12, it is many that the subculture seedling directly breaks up bud, the bud stalwartness, and growth is neat, and the leaf look dark green;
3, cultivate rooting rate and reach 99% with the root media seedling of taking root, when temperature is 25-28 ℃, can go out root about 15 days, well developed root system, on average going out the root amount has the 6-8 bar, can transplant in 30 days, and transplanting survival rate reaches 95.5%.
Embodiment
The invention will be further described with embodiment below, but the present invention is not limited to these embodiment.
Embodiment 1:
The red palm is with the numerous bud culture medium for tissue culture of bud, and this substratum comprises subculture medium and root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1800mg/L, ammonium nitrate 1350mg/L, Calcium dichloride dihydrate 350mg/L, magnesium sulfate heptahydrate 320mg/L, potassium primary phosphate 150mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, riboflavin (VB
2) 6.0mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.5mg/L, naphthylacetic acid 0.2mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 900mg/L, ammonium nitrate 675mg/L, Calcium dichloride dihydrate 175mg/L, magnesium sulfate heptahydrate 160mg/L, potassium primary phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.3mg/L;
Surplus is distilled water.
Embodiment 2:
The red palm is with the numerous bud culture medium for tissue culture of bud, and this substratum comprises subculture medium and root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1800mg/L, ammonium nitrate 1350mg/L, Calcium dichloride dihydrate 350mg/L, magnesium sulfate heptahydrate 320mg/L, potassium primary phosphate 150mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, riboflavin (VB
2) 6.0mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 2.0mg/L, naphthylacetic acid 0.8mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 900mg/L, ammonium nitrate 675mg/L, Calcium dichloride dihydrate 175mg/L, magnesium sulfate heptahydrate 160mg/L, potassium primary phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.6mg/L;
Surplus is distilled water.
Embodiment 3:
The red palm is with the numerous bud culture medium for tissue culture of bud, and this substratum comprises subculture medium and root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1800mg/L, ammonium nitrate 1350mg/L, Calcium dichloride dihydrate 350mg/L, magnesium sulfate heptahydrate 320mg/L, potassium primary phosphate 150mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, riboflavin (VB
2) 6.0mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.5mg/L, naphthylacetic acid 0.2mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 900mg/L, ammonium nitrate 675mg/L, Calcium dichloride dihydrate 175mg/L, magnesium sulfate heptahydrate 160mg/L, potassium primary phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.3mg/L;
Surplus is distilled water.
Embodiment 4:
The red palm is with the numerous bud culture medium for tissue culture of bud, and this substratum comprises subculture medium and root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1800mg/L, ammonium nitrate 1350mg/L, Calcium dichloride dihydrate 350mg/L, magnesium sulfate heptahydrate 320mg/L, potassium primary phosphate 150mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, riboflavin (VB
2) VB
26.0mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 2.0mg/L, naphthylacetic acid 0.8mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 900mg/L, ammonium nitrate 675mg/L, Calcium dichloride dihydrate 175mg/L, magnesium sulfate heptahydrate 160mg/L, potassium primary phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.6mg/L;
Surplus is distilled water.
Comparative Examples:
Obtain the substratum of aseptic bud by callus, this substratum comprises inducing culture, subculture medium and root media, wherein:
Component and each components contents of every liter of (L) inducing culture are as follows:
(1) macroelement: saltpetre 1900mg/L, ammonium nitrate 1650mg/L, Calcium dichloride dihydrate 400mg/L, magnesium sulfate heptahydrate 370mg/L, potassium primary phosphate 170mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L;
(3) organism: inositol 100.0mg/L, glycine 2.0mg/L, pyridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.8-1.2mg/L, 2,4 dichlorophenoxyacetic acid (2,4-D) 0.1-0.2mg/L;
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1900mg/L, ammonium nitrate 1650mg/L, Calcium dichloride dihydrate 400mg/L, magnesium sulfate heptahydrate 370mg/L, potassium primary phosphate 170mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.25mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L;
(3) organism: inositol 100.0mg/L, glycine 2.0mg/L, pyridoxine hydrochloride (VB
6) 0.5mg/L, nicotinic acid 0.5mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 1.5-2.0mg/L, kinetin (KT) 0.5-1.0mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 950mg/L, ammonium nitrate 825mg/L, Calcium dichloride dihydrate 200mg/L, magnesium sulfate heptahydrate 185mg/L, potassium primary phosphate 85mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.3-0.6mg/L;
Surplus is distilled water.
The substratum that utilizes above-described embodiment 1 and Comparative Examples to make carries out tissue culture to the red palm, and from the result of following table as can be seen, the technical indicator of embodiment far surpasses Comparative Examples.
Claims (1)
1. a red palm is with the numerous bud culture medium for tissue culture of bud, and this substratum comprises subculture medium and root media, it is characterized in that:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 1800mg/L, ammonium nitrate 1350mg/L, Calcium dichloride dihydrate 350mg/L, magnesium sulfate heptahydrate 320mg/L, potassium primary phosphate 150mg/L, iron vitriol 27.5mg/L, disodium ethylene diamine tetraacetate 37.3mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6.0mg/L, Sodium Molybdate Dihydrate 0.2mg/L, potassiumiodide 0.83mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, riboflavin (VB
2) 6.0mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L, vitamin (VB
1) 0.1mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.5-2.0mg/L, naphthylacetic acid 0.2-0.8mg/L;
Surplus is distilled water;
Component and each components contents of every liter of (L) root media are as follows:
(1) macroelement: saltpetre 900mg/L, ammonium nitrate 675mg/L, Calcium dichloride dihydrate 175mg/L, magnesium sulfate heptahydrate 160mg/L, potassium primary phosphate 75mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, iron vitriol 27.5mg/L;
(2) trace element: four water manganous sulfate 21.5mg/L, Zinc Sulphate Heptahydrate 8.3mg/L, boric acid 6mg/L, potassiumiodide 0.83mg/L, Sodium Molybdate Dihydrate 0.2mg/L, cupric sulfate pentahydrate 0.02mg/L, CoCL2 0.02mg/L;
(3) organism: inositol 80.0mg/L, glycine 2.5mg/L, vitamin (VB
1) 0.1mg/L, pyridoxine hydrochloride (VB
6) 0.45mg/L, nicotinic acid 0.45mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.3-0.6mg/L;
Surplus is distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210363349 CN102823503B (en) | 2012-09-26 | 2012-09-26 | Tissue culture medium for propagating anthurium buds by using buds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210363349 CN102823503B (en) | 2012-09-26 | 2012-09-26 | Tissue culture medium for propagating anthurium buds by using buds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102823503A CN102823503A (en) | 2012-12-19 |
| CN102823503B true CN102823503B (en) | 2013-08-14 |
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| CN103011969B (en) * | 2012-12-25 | 2014-11-05 | 浙江农林大学 | Medium specialized for plant tissue culture, and preparation method thereof |
| CN104663465A (en) * | 2015-03-31 | 2015-06-03 | 桂林市一峰食品有限公司 | Culture medium for heartleaf houttuynia herb tissue culture |
| CN104719167A (en) * | 2015-03-31 | 2015-06-24 | 桂林市一峰食品有限公司 | Tissue culture medium for herba houttuyniae |
| CN105010141A (en) * | 2015-07-09 | 2015-11-04 | 虞龙 | Anthurium rapid propagation method |
| CN106900555B (en) * | 2017-03-21 | 2018-10-09 | 钦州市林业科学研究所 | Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method |
| CN109906938B (en) * | 2019-03-07 | 2021-10-29 | 三明市农业科学研究院 | Anthurium germplasm resource in vitro preservation method |
| CN114208670B (en) * | 2021-12-16 | 2023-03-03 | 四川韵然景观园林工程有限公司 | Culture medium and culture method for fast propagation of woody flower plants |
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| CN1274206C (en) * | 2004-12-02 | 2006-09-13 | 广东省珠海市园艺研究所 | Tissue cultivating fast reproducing method for pot culturing red palm seedling |
| CN100424169C (en) * | 2005-01-31 | 2008-10-08 | 肖尊安 | Culture medium for anthurium andraeanum tissue culture and tissue culture seedling breeding method |
| CN101642053A (en) * | 2009-08-25 | 2010-02-10 | 浙江大学 | Cold-resistant anthurium induction culturing method |
| CN101785429B (en) * | 2009-12-01 | 2012-06-20 | 东莞市生物技术研究所 | Method for improving rapid differentiation and bud formation of anthurium andraeanum callus |
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