CN1274206C - Tissue cultivating fast reproducing method for pot culturing red palm seedling - Google Patents
Tissue cultivating fast reproducing method for pot culturing red palm seedling Download PDFInfo
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- CN1274206C CN1274206C CN 200410052480 CN200410052480A CN1274206C CN 1274206 C CN1274206 C CN 1274206C CN 200410052480 CN200410052480 CN 200410052480 CN 200410052480 A CN200410052480 A CN 200410052480A CN 1274206 C CN1274206 C CN 1274206C
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Abstract
The present invention discloses a fast tissue culture propagation method for pot culturing red palm seedlings. The aim of the present invention is to provide a fast tissue culture propagation method for pot culturing red palm seedlings, which has the advantages of high propagation speed and low cost. In the method, the young leaves of red palms are pretreated in a pretreatment fluid with BA and 2, 4-D after being sterilized; the young leaves of red palms are diced and inoculated in an improved MS inducing culture medium with 6-BA and 2.4-D to induce calluses, the blades of the calluses induced are diced and put in an improved MS differential culture medium with BA and NAA or an improved MS differential culture medium with TDZ to differentiate adventitious buds, the adventitious buds are put in a 1/2MS proliferation culture medium wit 6-BA or a 1/2MS proliferation culture medium with TDZ to culture rooting aseptic seedlings, the aseptic seedlings are transported under the optimum condition, and the improved MS comprises a large amount of 1/2MS elements and other MS ingredients of normal quantities. The present invention is applied to the field of flower breeding.
Description
Technical field
The present invention relates to a kind of potted anthurium andraeanum seedling tissue culture and rapid propagation method.
Background technology
The cutting cuttage of the Cai Yonging method of breeding in the past, the red palm of a strain can only breed 3～4 strains in 1 year, because of its reproduction coefficient is low, far can not meet the need of market.Therefore, the propagation method of potted anthurium andraeanum adopts tissue culture method more at present, it is a kind of totipotency of utilizing plant cell, the little block organization of cultured in vitro plant, carry out the fast numerous industrial breeding technique of plant clone, concrete technology is: downcut the young leaflet tablet of the red palm, induce callus earlier, differentiate indefinite bud again, cultivate the strong sprout that to transplant domestication again.This process need is more than 6 months generally speaking, and needs higher production cost (comprise culture medium cost and condition of culture expend etc.).So still there is following defective in existing potted anthurium andraeanum seedling propagation technique: reproduction speed is slow, cost is higher.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and the potted anthurium andraeanum seedling that a kind of reproduction speed is fast, cost is low tissue culture and rapid propagation method is provided.
The technical solution adopted in the present invention is: potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 2～5 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, mercuric chloride is handled and was taken out in 7～8 minutes in 0.1% mercuric chloride solution again, with standby in the immersion sterile water behind the aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 23～27 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 30～60 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with the improvement MS of BA 0.5mg/l, NAA 0.1mg/l or is added with TDZ 0.02mg/l;
(2) cultivation temperature is that 23～27 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is to be added with the 1/2MS of 6-BA0.1～0.5mg/l or to be added with TDZ0.01～0.02mg/l and the 1/2MS of 0.3% active carbon;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 2～4 centimetres of plant heights, 0.7～0.9 centimetre of maximum width of blade and have 2～3 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keeping temperature is 20～28 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 85～95%.
The invention has the beneficial effects as follows: be added with the improvement MS of 6-BA 1.0mg/l, 2.4-D0.1mg/l as inducing culture because the present invention adopts, described improvement MS is 1/2MS macroelement and other MS compositions of constant, and go forward earlier with being added with BA2.5mg/l and 2 the blade stripping and slicing being inoculated in described inducing culture, the pretreatment fluid of 4-D 2.0mg/l was handled 20 minutes, so can effectively promote the inductivity of red palm blade callus and shorten induction time; Because the improvement MS that utilization of the present invention is added with the improvement MS of BA 0.5mg/l, NAA 0.1mg/l or is added with TDZ 0.02mg/l differentiates indefinite bud as differential medium fast so can improve callus; Again because the present invention adopts the 1/2MS of active carbon be added with the 1/2MS of 6-BA0.1～0.5mg/l or be added with TDZ 0.01～0.02mg/l and 0.3% as proliferated culture medium, indefinite bud can both vigorously be bred on these two kinds of medium, and aseptic seedling can both form aerial root in the enrichment culture, has absorption function, can be directly used in domestication and transplant, so enrichment culture is quick.The present invention induces by shortening and time of enrichment culture, simplify technologies such as condition of culture or training method, can reduce production costs, improve efficient and profit that commercialization is produced, therefore fast, cost is low with potted anthurium andraeanum seedling tissue culture and rapid propagation method reproduction speed of the present invention.
Embodiment
Embodiment one:
Potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 2 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle and took out in 7 minutes again, with standby in the immersion sterile water behind the aseptic water washing 5 times, blade is immersed in the sterile water, be in order to prevent brownization of blade;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, sterile water is put in described blade stripping and slicing, insert in batch in the culture dish treating;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 23 ℃, the formation of evoked callus under the continuous darkness condition, after callus is induced under dark condition and was grown 60 days, increase illumination subsequently gradually, blade base ratio near petiole is easier to evoked callus, grow after 30 days, can induce callus at blade edge.
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with BA 0.5mg/l, NAA 0.1mg/l;
(2) cultivation temperature is that 23 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is the 1/2MS that is added with 6-BA0.1mg/l; Aseptic seedling can both form aerial root in the enrichment culture, has absorption function, can be directly used in domestication and transplant;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 2 centimetres of plant heights, 0.7 centimetre of maximum width of blade and have 2 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keep temperature to be 20 ℃ and also note shading to keep the low light condition low light condition, first week after the transplanting covers with film, and controlled humidity is that 85%, 30 day survival rate can reach more than 95%.
The present invention induces by shortening and time of enrichment culture, simplify technologies such as condition of culture or training method, can reduce production costs, improve efficient and profit that commercialization is produced, therefore fast, cost is low with potted anthurium andraeanum seedling tissue culture and rapid propagation method reproduction speed of the present invention.
Embodiment two:
Potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 5 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle and took out in 8 minutes again, with standby in the immersion sterile water behind the aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 27 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 60 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with BA 0.5mg/l, NAA 0.1mg/l;
(2) cultivation temperature is that 27 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is the 1/2MS that is added with 6-BA0.5mg/l; On the 6-BA medium that adds 0.1mg/l, plant height is higher than the 6-BA of 0.5mg/l, but growth coefficient is lower than BA 0.5mg/l;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 4 centimetres of plant heights, 0.9 centimetre of maximum width of blade and have 3 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keeping temperature is 28 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 95%.
All the other features are with embodiment one.
Embodiment three:
Potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 4 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle and took out in 7 minutes again, with standby in the immersion sterile water behind the aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 25 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 45 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with BA 0.5mg/l, NAA 0.1mg/l;
(2) cultivation temperature is that 25 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is the 1/2MS that is added with 6-BA0.3mg/l;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 3 centimetres of plant heights, 0.8 centimetre of maximum width of blade and have 2 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keeping temperature is 24 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 90%.
All the other features are with embodiment one.
Embodiment four:
Potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 2 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle and took out in 7 minutes again, with standby in the immersion sterile water behind the aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 23 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 30 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with TDZ 0.02mg/l;
(2) cultivation temperature is that 25 ℃, illumination are 12 hours every days;
The differential medium differentiation effect of adding TDZ is better, just can induce most of callus to break up in 30 days.
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, described proliferated culture medium is the 1/2MS that is added with the active carbon of TDZ 0.01mg/l and 0.3%, it is more vigorous to add TDZ propagation, and plant strain growth is very fast, the active carbon of interpolation 0.3% can improve the growth conditions of plant in the propagation training base in addition, plays the purpose of strong seedling culture.
(4) domestication of aseptic seedling is transplanted
(1) when growing to 2 centimetres of plant heights, 0.7 centimetre of maximum width of blade and have 2 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(5) keeping temperature is 20 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 85%.
All the other features are with embodiment one.
Embodiment five:
Potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 5 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle and took out in 8 minutes again, with standby in the immersion sterile water behind the aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 27 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 60 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with TDZ 0.02mg/l;
(2) cultivation temperature is that 27 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is the 1/2MS that is added with the active carbon of TDZ 0.02mg/l and 0.3%;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 4 centimetres of plant heights, 0.9 centimetre of maximum width of blade and have 3 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keeping temperature is 28 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 95%.
All the other features are with embodiment four.
Embodiment six:
Potted anthurium andraeanum seedling tissue culture and rapid propagation method of the present invention may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 4 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle and took out in 8 minutes again, with standby in the immersion sterile water behind the aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 25 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 45 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with TDZ 0.02mg/l;
(2) cultivation temperature is that 25 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is the 1/2MS that is added with the active carbon of TDZ 0.015mg/l and 0.3%;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 3 centimetres of plant heights, 0.8 centimetre of maximum width of blade and have 3 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keeping temperature is 24 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 90%.
All the other features are with embodiment four.
Claims (1)
1, a kind of potted anthurium andraeanum seedling tissue culture and rapid propagation method, it is characterized in that: it mainly may further comprise the steps:
(1) callus induces
(1) gets the red palm and launch 2～5 days young leaflet tablet fully as explant;
(2) with described blade earlier with 75% alcohol handle 15 seconds, in 0.1% mercuric chloride solution, handle taking-ups in 7～8 minutes again, immerse in the sterile water standby behind the usefulness aseptic water washing 5 times;
(3) the described blade after mercuric chloride is handled is with being added with BA2.5mg/l and 2, the pretreatment fluid processing of 4-D 2.0mg/l 20 minutes;
(4) pretreated described blade is cut into the blade stripping and slicing of 0.6cm * 0.6cm, it is standby that sterile water is put in described blade stripping and slicing;
(5) described blade stripping and slicing and being inoculated on the inducing culture is in batch cultivated, keep the blade face of described blade stripping and slicing to make progress during inoculation, described inducing culture is the improvement MS that is added with 6-BA 1.0mg/l, 2.4-D 0.1mg/l, and described improvement MS is 1/2MS macroelement and other MS compositions of constant;
(6) cultivation temperature is 23～27 ℃, induces under the continuous darkness condition 60 days earlier, increases illumination and regeneration subsequently gradually long 30～60 days, can induce callus at the edge of described blade stripping and slicing;
(2) indefinite bud induces and breaks up
(1) the described callus that will induce places to induce on the differential medium and differentiates indefinite bud, and described differential medium is the improvement MS that is added with the improvement MS of BA 0.5mg/l, NAA 0.1mg/l or is added with TDZ 0.02mg/l;
(2) cultivation temperature is that 23～27 ℃, illumination are 12 hours every days;
(3) enrichment culture of aseptic seedling
The described indefinite bud that differentiates is placed the aseptic seedling that enrichment culture goes out to take root on the proliferated culture medium, and described proliferated culture medium is to be added with the 1/2MS of 6-BA0.1～0.5mg/l or to be added with TDZ0.01～0.02mg/l and the 1/2MS of 0.3% active carbon;
(4) domestication of aseptic seedling is transplanted
(1) when growing to 2～4 centimetres of plant heights, 0.7～0.9 centimetre of maximum width of blade and have 2～3 sturdy, described aseptic seedling can transplant domestication;
(2) the last week is opened bottle cap so that described aseptic seedling progressively adapts to external condition in transplanting;
(3) clean described aseptic seedling root agar and be transplanted into peat soil and the perlite ratio is in 4: 1 the matrix;
(4) keeping temperature is 20～28 ℃, low light condition, and first week after the transplanting covers with film, and controlled humidity is 85～95%.
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| CN 200410052480 CN1274206C (en) | 2004-12-02 | 2004-12-02 | Tissue cultivating fast reproducing method for pot culturing red palm seedling |
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| CN 200410052480 CN1274206C (en) | 2004-12-02 | 2004-12-02 | Tissue cultivating fast reproducing method for pot culturing red palm seedling |
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| CN102823503B (en) * | 2012-09-26 | 2013-08-14 | 钦州市林业科学研究所 | Tissue culture medium for propagating anthurium buds by using buds |
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| CN103734009B (en) * | 2013-12-20 | 2015-06-17 | 三明市农业科学研究院 | Tissue culture method of anthurium |
| CN104542276B (en) * | 2014-11-10 | 2016-04-27 | 浙江省萧山棉麻研究所 | A kind of method of red palm virus-free culture |
| CN104488540A (en) * | 2015-01-15 | 2015-04-08 | 四川德昌县拓鑫城市园林绿化工程有限公司 | Anthurium planting method |
| CN114097617A (en) * | 2021-11-30 | 2022-03-01 | 南通大学 | Anthurium callus induction method |
| CN114287343A (en) * | 2022-01-05 | 2022-04-08 | 新疆维吾尔自治区阿克苏地区林业技术推广服务中心 | Tissue culture rapid propagation culture medium for anthurium andraeanum and tissue culture rapid propagation seed production method |
| CN115517169B (en) * | 2022-09-30 | 2023-05-12 | 南通大学 | Method for shortening tissue culture period of anthurium andraeanum |
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2004
- 2004-12-02 CN CN 200410052480 patent/CN1274206C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104024164A (en) * | 2011-12-16 | 2014-09-03 | 三菱丽阳株式会社 | Purification system and filter |
| CN104024164B (en) * | 2011-12-16 | 2017-03-29 | 三菱丽阳株式会社 | Cleaning system and filter |
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