CN1706239A - Walnut twig cutting propagation technology - Google Patents
Walnut twig cutting propagation technology Download PDFInfo
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- CN1706239A CN1706239A CN 200410009201 CN200410009201A CN1706239A CN 1706239 A CN1706239 A CN 1706239A CN 200410009201 CN200410009201 CN 200410009201 CN 200410009201 A CN200410009201 A CN 200410009201A CN 1706239 A CN1706239 A CN 1706239A
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- 235000009496 Juglans regia Nutrition 0.000 title abstract description 39
- 235000020234 walnut Nutrition 0.000 title description 37
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- 238000000034 method Methods 0.000 claims description 34
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Abstract
The present invention provides the technology of combining grafting technology and cutting propagation technology. By means of rejuvenapion, yellowing, inducing rooting with growth regulator, special treatment of scion, and subsequent culturing and transplanting, the present invention realizes the industrial asexual propagation of walnut tree. The present invention has simple operation, low cost and high production efficiency, and is suitable for industrial production.
Description
Technical field
The present invention relates to the take root vegetative propagation technique of seeds of difficulty, specifically, belong to Juglans (Juglans sp.) trees softwood cutting propagation technology.
Background information
The cultivation history of walnut (Juglans regia L.) is long, has the good reputation first of the four big dry fruits.Walnut is great sphere economy seeds, because its good economy, ecology and social benefit are extensively cultivated in countries in the world.
The present situation of China's walnut production be area big, yield poorly, poor quality.From the sixties in last century, China has carried out walnut and has selected excellent cooperation and prevalent variety cultivation tackling key problem, cultivates a series of improved variety of walnut, also cultivates some walnuts and iron walnut interspecific hybrid, for China's improved variety of walnutization is laid basis preferably.After tentatively having solved the improved variety of walnut problem; walnut vegetative propagation difficulty is presented in the improved variety of walnut process again highlightedly; become the main factor of its development of restriction, show that improved variety of walnut continues to bring out on the one hand, the kind that is used for large-scale production on the other hand is very few.
Walnut vegetative propagation difficulty shows, conventional graft survival rate instability, reproduction coefficient is low, cuttage shortcoming such as be difficult for taking root, even conventional grafting survival, shortcomings such as stock and scion do not match on the nutrition form, and the walnut after growing up to has wood quality poor, and fruiting rate is low, add that most breedings are real type morning, form the poor ability of long shoot, cause scion output not satisfy the needs of propagation by grafiting far away, the popularization of new varieties is very limited.Therefore, solve the fast numerous problem of improved variety of walnut and seem particularly urgent.
At walnut vegetative propagation present situation, forefathers have carried out some researchs, particularly along with the continuous development of plant tissue culture technique and perfect, are fast numerous a kind of new method and the good research system of providing of walnut.Aspect cottage propagation puts into practice,, all obtain comparatively satisfied result although adopted various technical measures.There is report to introduce the walnut kind, adopts two-step method (dark cultivate, light cultivate) successfully to induce and taken root, realized the test tube vegetative propagation through many successive transfer culture in vitro.Yet this technology exists apparatus expensive, technical sophistication, the more high deficiency of production cost, is difficult in the production unit promotion and application
Summary of the invention
The present invention is directed to the Juglans plant establishment difficulty that need to solve, the cottage propagation coefficient is little, and the technical barrier that transplanting success is low provides a kind of vegetative technology of Juglans plant of simple to operate, with low cost, stability and high efficiency.
Purpose of the present invention can realize by following method:
1, provides a kind of graft technology and the cuttage technique vegetative propagation technique of combination organically, root induction technology by multiple young technology, yellow technology, growth regulator, the fringe bar is carried out specially treated, realize the industrialization vegetative propagation technique of Juglans trees.
2, according to above 1 described Juglans trees softwood cutting propagation technology, this technology step be:
1) the multiple children of vegetable material handles;
2) bury dried and the yellow processing;
3) preparation of growth regulator and use;
4) cottage breeding;
5) transplant.
3, according to the step of the Juglans trees softwood cutting propagation technology under above-mentioned 2, wherein the multiple young treatment step of vegetable material is:
1. select scion: it is full to choose health, with the scion of art methods cultivation.
2. with the scion grafting chosen on the stock of annual base footpath greater than 1cm, (select for use this anvil survival rate the highest) through the cultivation in 1 year, annual grafting, just be used for cultivating female branch of the used fringe bar of cuttage.
The health standards of the grafting that generates: annual grafting terminal bud is full, dark green leaf color, and more than the 1cm, branch is sturdy for ground stem (grafting interface), complete lignification, well developed root system, no damage by disease and insect.
4,, wherein bury and do and step that yellow is handled is according to the step of above-mentioned 2 described Juglans trees softwood cutting propagation technology:
1. from the above-mentioned multiple children's of the above clip of interface annual grafting, lie on the sand, cover husky 4～5cm and bury dried, germinated spray after 15～20 days, every root graft seedling is about 80～120cm, 2～3 of per 4～5cm germinating tender shoots, continue to cover sapin and bury, promote the spray base portion yellow of germinating; Generally bury and did the back 20～40 days, treat that every spray grows 4～5 pairs of compound leaves, when the spray base portion had the position of the obvious yellow of 4cm because covering sand, promptly yellow was qualified at last.
2. more than interface, the clip semi-lignified, healthy and strong no damage by disease and insect, diameter is about 0.4～0.6cm, and length is the spray of 5～10cm, as the cuttage material.
3. cuttage front vane processing method: cut off whole blades of the above 3～4cm of plugged ear base portion, it is 1/2 stand-by that rest blade is wiped out.
Bury to do and can also connect that stock is flat together to be buried, method and step are the same.
5, according to the step of above-mentioned 2 described Juglans trees softwood cutting propagation technology, wherein the preparation of growth regulator and using method are:
1. the preparation of growth regulator:
Used growth regulator can be that IBA solution also can be ABT rooting powder solution, IAA solution, NAA solution, and valid density is 0.25～4mgml
-1Its compound method is as follows:
The 4mg growth regulator is dissolved in the alcohol of 500ml 75% with glass beaker, adds 500ml distilled water again, the gained original liquid concentration is 4000ppm;
Get the above-mentioned stoste 100ml for preparing, add distilled water 0～1500ml, mix, be required 0.25～4mgml
-1The growth regulating agent solution.
2. the using method of growth regulator: spray plugged ear base portion is carried out speed dip in processing in the growth regulator for preparing, the time that speed is dipped in is 2～3 seconds.
6. cottage breeding:
1. prepare to insert earth: insert earth and mix with the equal-volume ratio, insert earth and sterilize with bactericide for cow dung and rough sawn end (the chaff skin that becomes thoroughly decomposed of diameter 2～4mm or 4～5mm) that fully becomes thoroughly decomposed.
Bactericide can be that 2000 times of carbendazols also can be the various bactericide of prior art formula.
2. utilize the prior art cuttage in slotting machine ready spray.
3. slotting machine is arc and covers with plastic foil, adopts canopy to shade and spray cooling outward, and light intensity is 17.1～38.0% of full sun in the canopy, 15～28 ℃ of slotting machine temperature, and inserting earth temperature April is 7～10 ℃, and be 15～25 ℃ May, and be 18～26 ℃ June; Relative moisture remains on 85%～98% in the canopy; Insert earth moisture content about 95%.
7, transplant
1. transplant in the greenhouse
A high light hardening: 1020Lx is advisable with light intensity, 14 days posterior lobe looks obviously add be deep to dark green, the blade thickening, resistance strengthens, and is beneficial to transplant survival.The high light hardening is the key link of decision transplanting survival rate.
The selection of b transplanting medium: vermiculite+turfy soil is that 2: 3 ratio is mixed with volume ratio, stirs, and this kind matrix good water-retaining property, good permeability, more satisfactory; Soil humidity: 82～85%.
The control of c environmental condition: 25 ± 3 ℃ of temperature, humidity is controlled at about 95%.
2. field-transplanting
Transplanted seedling is grown in the greenhouse and can be moved into the land for growing field crops in 40 days, blade face water spray means routinely, and spray 2～3 preceding 7～10 day every day, and per 3～5 days water sprays are once after 7～10 days.The initial stage field management mainly is water management, and blade face evaporation this moment is fast and the shoot root water absorbing capacity is poor, adopts blade face water spray mode, is the effective ways that keep water balance in the transplanted seedling body.
Do not advocate fertilising before the transplanting, transplanting after 1 week can foliar application, after about 3～4 weeks, just can manage according to general field seedling raising technical specification.
8. this technology also can be used for the take root vegetative propagation of seeds of other difficulty.
Below the present invention is described further:
The multiple children of above-mentioned vegetable material handles and bury dried yellow can promote the spray cuttage rooting, and its influence sees Table 1
Table 1
| Handle | Rooting rate (%) |
| The walnut seedling of handling without multiple children buries dried | ??0b |
| Multiple young grafting | ??0b |
| Multiple young grafting is buried dried | ??85a |
(illustrate: rooting rate The data percentage difference significance compares, and other The data GraphPad3.0 software compares, and significance, the different significance level that reaches of lowercase different table differential are determined in the Dunnett's test)
The relation of above-mentioned yellow processing and cuttage root-taking is the example explanation with unusual walnut (Paradox, J.regia x J.hansis) and No. 1 walnut in Liaoning (Juglans regia L.).
Burying during dried (comprising yellow) handle, the yellow of spray base portion or not yellow epicormic branch cuttage is survived influence obviously.See the influence of Fig. 1 base portion yellow to the spray cuttage rooting
As seen from Figure 1, base portion is No. 1 walnut of unusual walnut and Liaoning of yellow not, and the rooting rate of spray is respectively 15.8% and 5%, the base portion yellow be respectively 93.3% and 69.17%.Find also that in test if cover Sha Taihou, surpassing 6cm can influence the sprouting and the growth of burying dried seedling lateral bud, therefore covers husky thickness and is advisable with 4～5cm.
The step of above-mentioned Juglans trees softwood cutting propagation technology, wherein the preparation of growth regulator and using method are:
IBA solution: 4mgIBA is dissolved in the alcohol of 500ml 75% with glass beaker, adds 500ml distilled water again, the gained original liquid concentration is 4000ppm.Determine extension rate according to the test needs during use, just can obtain the IBA solution of variable concentrations, get the above-mentioned stoste 100ml for preparing, add distilled water to 0～1500ml, mix, be required 0.25～4mgml
-1The growth regulating agent solution.To toing many or too much for use at that time or no stoste should install with Brown Glass Brown glass bottles and jars only, be placed on 5 ℃ of following lucifuge places and preserve.
The ABT rooting powder solution: prescription and compound method are the same
IAA solution: prescription and compound method are the same
NAA solution: prescription and compound method are the same
Different growth regulators is as shown in table 2 to the influence of the situation of taking root:
Table 2: No. 1 walnut in Liaoning is handled the situation of on average taking root with different growth regulators
| The growth regulator type | Concentration 1 (ppm) | Rooting rate (%) | Concentration 2 (ppm) | Rooting rate (%) | Concentration 2 (ppm) | Rooting rate (%) |
| ??IBA | ??500 | ??85 | ??1000 | ??80 | ??2000 | ??60 |
| ??ABT | ??500 | ??74 | ??1000 | ??77 | ??2000 | ??34 |
| ??IAA | ??500 | ??52 | ??1000 | ??45 | ??2000 | ??21 |
| ??NAA | ??500 | ??36 | ??1000 | ??23 | ??2000 | ??15 |
As can be seen from Table 2, plant growth regulator most preferably is IBA, its effect stability, and effect is obviously.
The growth regulator of variable concentrations also has very big influence to the situation of taking root, and is example with IBA solution.
The influence that table 3 variable concentrations IBA solution-treated is taken root and healed epicormic branch cuttage
| ??IBA(mg· ??ml -1) | Wound healing rate (%) | Lethality (%) | Rooting rate (%) |
| ??0 | ??0d | ??77.5a | ??0d |
| ??0.25 | ??20.0c | ??50.3b | ??15.0c |
| ??0.35 | ??75.6b | ??22.4c | ??38.2b |
| ??0.45 | ??85.6b | ??0e | ??91.8a |
| ??0.5 | ??90.0a | ??0e | ??93.3a |
| ??1 | ??92.5a | ??5.75d | ??86.5a |
| ??2 | ??93.8a | ??0e | ??87.5a |
| ??4 | ??81.3b | ??14.5c | ??41.4b |
| ??8 | ??75.8b | ??24.0c | ??0d |
(illustrate: wound healing rate and rooting rate The data percentage difference significance compare, and other The data GraphPad3.0 software compares, and significance, the different significance level that reaches of lowercase different table differential are determined in Dunnett ' s test)
As can be seen from Table 3, the concentration of IBA solution is at 0.25～4mgml
-1During scope, best to the effect that epicormic branch cuttage is taken root and healed.
Plant growth regulator: medicine name: IBA (indolebutyric acid)/IAA (heteroauxin)/NAA (methyl); Registration mark: SIGMA; Agriculture chemical registration number: 133-32-4 EC N0 205-105-5; Manufacturer: SIGMA CHEMICALC0.; Dealer: Beijing Hui Bei Bioisystech Co., Ltd.
The Juglans seeds survival rate that the technology of the present invention is cultivated reaches 68-92%
This technology also can be used for the take root vegetative propagation of seeds of other difficulty.
Multiple children's grafting is buried dried, yellow, and the technology of hestening rooting is applied to Juglans and some other difficulties respectively and takes root on the seeds, has all obtained the result of taking root preferably, and is as shown in table 4.
The application of table 4 Juglans trees softwood cutting propagation technology on Juglans and other difficulty are taken root seeds
| Seeds | Cuttage spray number | Rooting rate (%) | Seeds | Cuttage spray number | Rooting rate (%) | |
| Juglans mandshurica | ??80 | ??97.5 | Mandarin jacket wood | ??80 | ??86.3 | |
| Cathay hickory | ??62 | ??93.5 | Aspen | ??200 | ??98 | |
| Black walnut | ??76 | ??53.9 | Morello | ??90 | ??75.6 | |
| Chinese catalpa | ??60 | ??93.3 | Bedleaf cherry plum | ??70 | ??88.6 | |
| Three maos of poplars | ??85 | ??100 |
The present invention organically combines graft technology and cuttage technique, by the root induction technology of multiple young technology, yellow technology, growth regulator, specially treated is carried out in scion, realizes the industrialization vegetative propagation technique of Juglans trees.Method of operating of the present invention is easy, and conventional equipment and those of ordinary skill can be finished, and cost is low, and the production efficiency height is suitable for suitability for industrialized production.
Embodiment
The multiple children of embodiment 1 unusual walnut graft handles
1. select scion: choose healthy, 1000 of the unusual walnut grafts of cultivating with art methods.
2. with the scion grafting chosen on this anvil of annual base footpath greater than 1cm, through the cultivation in 1 year, annual grafting, just will be used for cultivating female branch of the used fringe bar of cuttage.Generate 982 of healthy graftings.
The health standards of the grafting that generates: annual grafting terminal bud is full, dark green leaf color, and more than the 1cm, branch is sturdy for ground stem (grafting finger mouth), complete lignification, well developed root system, no damage by disease and insect.
The multiple children of embodiment 2 cathay hickory (J.cathayensis Dode.) scion handles
Select scion: choose healthy, 1000 cathay hickory scion of cultivating with art methods.
With the scion grafting chosen on this anvil of annual base footpath greater than 1cm, through the cultivation in 1 year, annual grafting, just will be used for cultivating female branch of the used fringe bar of cuttage.Generate 976 of healthy graftings.
The health standards of the grafting that generates: annual grafting terminal bud is full, dark green leaf color, and more than the 1cm, branch is sturdy for ground stem (grafting finger mouth), complete lignification, well developed root system, no damage by disease and insect.
The multiple children of No. 1 walnut graft in embodiment 3 Liaoning handles
Select scion: choose healthy, 1000 of No. 1 walnut grafts in commercially available Liaoning of cultivating with art methods.Other operations generate 984 of healthy graftings with embodiment 1.
Embodiment 4 unusual walnut graftings are buried and do and the yellow processing
1. the multiple children's that embodiment 1 is cultivated the annual grafting of unusual walnut, the above branch of intercepting interface is put down and is buried (burying dried), bury the dried husky 4～5cm that covers, germinate spray on the branch that self-balancing is buried after 15～20 days, the approximately long 80～120cm of every root graft seedling branch, 2～3 eyes are arranged, spray of each eye germinating on the branch on every 5cm branch.Covering husky purpose is the spray base portion yellow that promotes germinating; Generally bury and did the back 20～40 days, every coppice shoot has 4～5 pairs of compound leaves, and when the spray base portion had the position of the obvious yellow of 4cm because covering sand, promptly yellow was qualified at last.
2. more than interface, the clip semi-lignified, healthy and strong no damage by disease and insect, diameter is about 0.4～0.6cm, and length is the spray of 7～10cm, and 982 root graft seedlings generate 50845 qualified yellow sprays, as the cuttage material.
3. cuttage front vane processing method: remove whole blades of the above 3～4cm of plugged ear base portion, it is 1/2 stand-by that rest blade is wiped out.
Embodiment 5 unusual walnut graftings are buried and do and the yellow processing
With embodiment 2 described multiple childrens' the annual grafting of cathay hickory, even stock is flat together buries (burying dried), and other step generates 62413 sprays that yellow is qualified, as the cuttage material with embodiment 4
Embodiment 6 unusual walnut graftings are buried and do and the yellow processing
With embodiment 1 described multiple children's the annual grafting of unusual walnut, even stock is flat together buries (burying dried), and other step generates 58426 sprays that yellow is qualified, as the cuttage material with embodiment 4.
The preparation and the use of embodiment 7 growth regulator IBA solution
1. the preparation of IBA solution: 4mgIBA is dissolved in the alcohol of 500ml75% with glass beaker, adds 500ml distilled water again, gained IBA original liquid concentration is 4000ppm.
Get the above-mentioned IBA stoste 100ml for preparing, add distilled water, mix, be required 0.5mgml to 700ml
-1IBA solution.
2. the using method of growth regulator: spray plugged ear base portion is carried out speed dip in processing in the growth regulator for preparing, the time that speed is dipped in is 2～3 seconds.
The preparation and the use of embodiment 8～11 growth regulator IBA solution
Preparation IBA stoste method is got the above-mentioned IBA stoste 100ml for preparing with embodiment 6, and preparation IBA solution methods adds the distillation water yield and the IBA solution concentration of preparing and sees the following form with embodiment 6, uses the IBA solution methods with embodiment 6.
| Embodiment 8 | Embodiment 9 | Embodiment 10 | Embodiment 11 | |
| Add the distillation water yield | ??0ml | ??100ml | ??200ml | ??400ml |
| The IBA solution concentration | ??4mg·ml -1 | ??2mg·ml -1 | ??1.33mg·ml -1 | ??0.8mg·ml |
| Embodiment 12 | Embodiment 13 | Embodiment 14 | Embodiment 15 | |
| Add the distillation water yield | ??500ml | ??790ml | ??1043ml | ??1500ml |
| The IBA solution concentration | ??0.67mg·ml -1 | ??0.45mg·ml -1 | ??0.35mg·ml -1 | ??0.25mg·ml -1 |
The cottage breeding of No. 1 walnut seedling in embodiment 16 Liaoning
1. prepare to insert earth: insert earth and mix with equal proportion (v/v), insert earth and sterilize with 2000 times of carbendazols for the cow dung fully become thoroughly decomposed and the chaff skin that becomes thoroughly decomposed of diameter 4～5mm.
With ready, through growth regulator 0.5mgml
-1IBA solution.1000 of Liaoning No. 1 walnut fringe bars that speed is dipped in processing utilize the prior art cuttage in slotting machine.
2. slotting machine covers with arc plastic foil, adopts canopy to shade and spray cooling outward, and light intensity is 17.1～38.0% of full sun in the canopy, 15～28 ℃ of slotting machine temperature, inserting earth temperature April is 7～10 ℃, be 15～25 ℃ May, be 18～26 ℃ June, and slotting earth temperature is relevant with season and weather; Relative moisture remains on 85%～98% in the canopy; Insert earth moisture content about 95%.
Through behind the cottage breeding, there are 820 offspring normally to take root, grow fine rooting rate 82%.
Transplanting seedlings of the embodiment 17 No. 1 walnut seedlings in Liaoning
1. transplant in the greenhouse
The selection of a transplanting medium: vermiculite+turfy soil is that 2: 3 ratio is mixed with volume ratio, stirs, and this kind matrix good water-retaining property, good permeability, more satisfactory; Soil humidity: 82～85%.
B is transplanted to No. 1 walnut seedling in Liaoning that cultivates in the foregoing description 16 in the greenhouse
C high light hardening: 1020Lx is advisable with light intensity, 14 days posterior lobe looks obviously add be deep to dark green, the blade thickening, resistance strengthens, and is beneficial to transplant survival.The high light hardening is the key link of decision transplanting survival rate.
The control of d environmental condition: 25 ± 3 ℃ of temperature, relative moisture is controlled at about 95%.
2. field-transplanting
Transplanted seedling is grown in the greenhouse and can be moved into the land for growing field crops in 40 days, and spray 2～3 preceding 7～10 big every days, and per 3～5 days water sprays once after 7～10 days.The initial stage field management mainly is water management, and blade face evaporation this moment is fast and the shoot root water absorbing capacity is poor, adopts blade face water spray mode, is the effective ways that keep water balance in the transplanted seedling body.
Do not advocate fertilising before the transplanting, transplanting after 1 week can foliar application, after about 3～4 weeks, just can manage according to general field seedling raising technical specification.Through the back detection of 8 weeks, survive 750 strains, seedling grows fine.
Claims (10)
1, a kind of Juglans trees softwood cutting propagation technology, it is characterized in that: grafting and cuttage technique are combined, by the root induction technology of multiple young technology, yellow technology, growth regulator, the fringe bar is carried out specially treated, realize the industrialization vegetative propagation technique of Juglans trees.
2, Juglans trees softwood cutting propagation technology according to claim 1 is characterized in that concrete steps are as follows:
1. the multiple children of vegetable material handles;
2. bury and do and the yellow processing;
3. the preparation of growth regulator and use;
4. cottage breeding;
5. transplant.
3, Juglans trees softwood cutting propagation technology according to claim 1 and 2 is characterized in that multiple children's processing of vegetable material is finished by following step:
1. select scion: choose healthy, the scion of cultivating with art methods;
2. with the scion grafting chosen on the stock of annual base footpath greater than 1cm, through the cultivation in 1 year, annual grafting, just be used to cultivate female branch of the used fringe bar of cuttage.
4, Juglans trees softwood cutting propagation technology according to claim 1 and 2, it is characterized in that burying do and step that yellow is handled as follows:
1. above-mentioned multiple children's annual grafting covers that husky 4～5cm is flat to be buried, and promotes the spray base portion yellow of germinating;
2. more than interface, the healthy no damage by disease and insect of clip, diameter is about 0.4～0.6cm, and length is the spray of 5～10cm, as the cuttage material;
3. cuttage front vane processing method: cut off whole blades of the above 3～4cm of plugged ear base portion, it is 1/2 stand-by that rest blade is wiped out.
5, Juglans trees softwood cutting propagation technology according to claim 1 and 2 is characterized in that used growth regulator can be that IBA solution also can be ABT rooting powder solution, IAA solution, NAA solution, and valid density is 0.25～4mgml
-1Its compound method is as follows:
1. the 4mg growth regulator is dissolved in the alcohol of 500ml 75% with glass beaker, adds 500ml distilled water again, the gained original liquid concentration is 4000ppm;
2. get the above-mentioned stoste 100ml for preparing, add distilled water 0～1500ml, mix, be required 0.25～4mgml
-1The growth regulating agent solution.
6, Juglans trees softwood cutting propagation technology according to claim 1 and 2, the using method that it is characterized in that used growth regulator are spray plugged ear base portion to be carried out speed dip in processing in the growth regulator for preparing.
7, Juglans trees softwood cutting propagation technology according to claim 1 or 5 is characterized in that growth regulator wherein is an IBA solution, and concentration is 0.25～4mgml
-1
8, Juglans trees softwood cutting propagation technology according to claim 1 and 2 is characterized in that the condition of cottage breeding and step are as follows:
1. prepare to insert earth: insert the chaff skin that becomes thoroughly decomposed that rough sawn end that earth is 2～4mm for the cow dung fully become thoroughly decomposed and diameter or diameter be 4～5mm with equal-volume than mixing, insert earth and sterilize with bactericide;
2. utilize the prior art cuttage in slotting machine the ready spray of handling through growth regulator; It is arc that slotting machine is, and covers with plastic foil, adopts canopy to shade and spray cooling outward, and light intensity is 17.1～38.0% of full sun in the canopy, 15～28 ℃ of slotting machine temperature, and inserting earth temperature April is 7～10 ℃, and be 15～25 ℃ May, and be 18～26 ℃ June; Relative moisture remains on 85%～98% in the canopy, inserts earth moisture content about 95%.
9, Juglans trees softwood cutting propagation technology according to claim 1 and 2, it is characterized in that transplanting seedlings is divided into greenhouse transplanting and two steps of field-transplanting, and actual conditions and step are as follows:
1. transplant in the greenhouse
A. the selection of transplanting medium: vermiculite+turfy soil is that 2: 3 ratio is mixed with volume ratio, stirs soil humidity: 82～85%;
B. high light hardening: 1020Lx is advisable with light intensity, 14 days posterior lobe looks obviously add be deep to dark green, the blade thickening, resistance strengthens, and is beneficial to transplant survival;
C. nurse young plants in hothouses: after the high light hardening, continue according to a conventional method to cultivate 26 days;
D. the control of environmental condition: 25 ± 3 ℃ of temperature, relative moisture is controlled at about 95%;
2. field-transplanting
Transplanted seedling is grown in the greenhouse and can be utilized routine techniques to move into the land for growing field crops in 40 days.
10, Juglans trees softwood cutting propagation technology according to claim 1 and 2 is characterized in that this technology also can be used for the take root vegetative propagation of seeds of other difficulty.
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