A kind of bulla walnut cuttage fast seedling-cultivating method
Technical field
The present invention relates to a kind of cultural method of bulla Juglans.
Background technology
Walnut (Juglans regia) is one of world-renowned four big dry fruits, is Juglandaceae juglans deciduous tree, in
State's cultivation has the history of more than 2000, forms many kinds and type, and walnut is rich in fatty (more than 70%) and protein
(20%) it is, high heat energy nutriment, is the green health care food of no cholesterol again, walnut is precious from head to foot, except walnut kernel
Have outside edibility, its trunk, branch, leaf, rascal etc. have certain value.Walnut or rocky mountainous area Rocky Desertification Control
The high seeds with the economic value of production of forestry.Walnut Industry be become mountain area resource inferior position into economic advantages, ecological dominance,
The economic industry of the rocky mountainous area people is benefited, is a comprehensive benefit, the ecological systems engineering of long-acting benign cycle;It is
One item collection economy, society, ecological triple effect are beneficial to integral integrated agriculture industry.However, walnut nursery is to suppress Walnut Industry
Bottleneck, walnut Sexual breed seedling growth cycle length, about 10 or so years could as a result, it is difficult to solve plant dwarfing problem, it is sexual
Reproduction characters of progenies segregation phenomenon is serious, and in improved seeds are cultivated and promoted, people are typically solved using grafting approach, and walnut
Grafting be subject to seasonal restrictions again, survival rate is low, technical requirements are high, cost is big, production management difficulty is big, the tissue cultures of walnut are deposited
In technical factors such as root inductions, take root undesirable, tissue culture is higher to technology, equipment requirement, and fund input amount is big, and obtain employment people
The problems such as member's professional technique requires high, extremely difficult one of seeds that take root of Juglans, and common cuttage can not take root limits its application
Promote.
At present, the growing and cultivation of bulla walnut mainly carries out nursery using traditional grafting and seedling from seed, raw
Long period is grown, and floor space is big, it is difficult to realizes efficiently quickly heavy industrialization growing and cultivation.
The content of the invention
The technical problem to be solved in the present invention is, there is provided a kind of bulla Juglans cultural method, overcomes prior art difficult
The defects of to realize efficiently quickly heavy industrialization growing and cultivation.
The technical solution adopted for the present invention to solve the technical problems is:A kind of bulla Juglans cultural method is provided, its
It is characterised by, comprises the following steps:
S1, fringe bar shearing:To give birth to the healthy and strong branch of bulla walnut then as material, it is clipped after fringe bar stay 2-3 bud
Point, upper end are cut flat, and lower end is smoothly cut into horse ear shape in the 45 degree of angles in place against axillary bud point, and otch can not be ftractureed, and 20-30 branch is bundled into
A bundle, lower end is neat, standby;
S2, fringe bar immersion and cuttage:Fringe bar lower cut is after flowing water cleans 3~5 hours, the potassium permanganate through 0.1-1%
Fringe bar sterilization is carried out, is transferred to after being soaked 6~12 hours in the solution containing appropriate plant growth regulator, what insertion had sterilized in advance
Perlite+peat (3:1) in matrix;
The soaking solution includes plant growth regulator, potassium permanganate, and compound method is:Potassium permanganate is the sterilization of fringe bar
Liquid, concentration 0.1-1%, appropriate plant growth regulator are formulated for 500-1500ppm;
S3, vernalization, culture of rootage:It is colonized 2 weeks posterior end otch and forms callus, winter is covered in Small plastic shed or greenhouse
Heating or GA carry out vernalization to fringe bar, bud is produced itself growth hormone, hestening rooting, foliar fertilizer are sprayed after lamina, promote
Photosynthesis, hestening rooting.15~25 DEG C of above-mentioned steps cultivation temperature, 1000~2000LUX of intensity of illumination, humidity is in 60-
80%;
S4, acclimatization and transplantses;
Step S2~S4 condition of culture:Temperature is 15~25 DEG C, 1000~2000LUX of intensity of illumination, light application time 6~
12 hours/day;
Step S4 condition of culture:By the walnut plantlet of transplant for having two level root to garden mould+peat (2:1) in matrix, hide
Screened negative is sheltered from heat or light, and humidity 60-70%, goes out garden within 50~90 days.
In the bulla Juglans cultural method of the present invention, the step S2, plant growth regulating that vernalization is taken root in S3
Agent is IBA sylvite and/or NAA, GA, the use of IBA-K concentration is 500-1500ppm, the use of NAA concentration is 100-500ppm,
The use of GA concentration is 50-100ppm.
In the bulla Juglans cultural method of the present invention, the potassium permanganate concentration that is used in step S1 for 0.1%~
1%.
In the bulla Juglans cultural method of the present invention, in step S3, about 2-8 centimetres of the length taken root, number of taking root is
10-20 bars.
In the bulla Juglans cultural method of the present invention, the cutting medium in step S3 is perlite+peat (3:1);
Cultivation temperature is 20 ± 2 DEG C, 1000~2000LUX of intensity of illumination.
In the bulla Juglans cultural method of the present invention, the plant establishment conditioning agent is IBA-K and/or NAA, is used
IBA-K concentration is 500-1500ppm, the use of NAA concentration is 100-500ppm.
The walnut vernalization conditioning agent GA, the use of GA concentration is 50- in the bulla Juglans cultural method of the present invention
100ppm。
Implement bulla Juglans cultural method of the present invention, compared with the prior art, it has the prominent advantages that:
The speed of growth and planting benefit greatly improve;The method bulla Juglans of the present invention start to take root for 40 days, average
Rooting rate mean elements 10~20, there is 2 grades of root systems, can go out garden within 3 months up to 50~70%;And existing bulla walnut grafting
The bud of method one can only be at most transferred on a stock, at most grow up to 1 young plant, growth cycle 1 year more than half;
Biennial seedling after cuttage root-taking, it can be taken root faster with Miao Fanmiao.
Introduces a collection can be made by the use of the branch cut every year as micro-material, reduce production cost, environmental pollution is small;
Technique is simpler, can produce throughout the year, is adapted to extensive nursery production.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment step:(1) fringe bar is sheared;(2) fringe bar immersion and cuttage;(3) with taking root, (4) hardening is moved for fringe bar vernalization
Plant, survival rate can go out garden up to 70-80%, three months.
A kind of bulla Juglans cultural method of the present invention comprises the following steps:
The first step, fringe bar shearing:To give birth to the healthy and strong branch of bulla walnut then as material, it is clipped after fringe bar stay 2-3
Individual axillary bud point, upper end are cut flat, and lower end is cut into horse ear shape against 45 degree of angles at axillary bud point, and otch can not ftracture, 20-30 branch bundle
Into a bundle, lower end is neat, standby;
Second step, fringe bar immersion:Fringe bar lower cut is after flowing water cleans 3~5 hours, the potassium permanganate through 0.1-1% enters
Row fringe bar sterilizes, and is transferred to after being soaked 6~12 hours in the solution containing appropriate plant growth regulator, the treasure that insertion has sterilized in advance
Zhu Yan+peat (3:1) in matrix;
The soaking solution includes plant growth regulator, potassium permanganate, and compound method is:Potassium permanganate is the sterilization of fringe bar
Liquid, concentration 0.1-1%, appropriate plant growth regulator are formulated for 500-1500ppm;
Plant growth growth regulator IBA-K:3- butyric acid potassiums, it is plant endogenous auxin, cell division can be promoted
With cell growth, the general entitled indole-3-butyric acid potassium plant cell cultur of English, molecule
Formula is C12H12KNO2。
Plant growth growth regulator NAA:1-Naphthaleneacetic acid, methyl α-naphthyl acetate, abbreviation NAA, it is a kind of
Auximone.
GA:Gibberellin, basic structure are gibberellanes.It is a kind of plant hormone being widely present.
3rd step, vernalization, culture of rootage:It is colonized 2 weeks posterior end otch and forms callus, winter carries out overlay film to fringe bar
Or heating or GA spray vernalization, bud is produced itself synthetic auxin, hestening rooting, foliar fertilizer are sprayed after lamina, promote
Photosynthesis, hestening rooting, using the present invention 50 days after walnut rooting rate up to 50-70%, several 10-20 bars of taking root, root long 2-8
Centimetre, start have two level root.15~25 DEG C of above-mentioned steps cultivation temperature, 1000~2000LUX of intensity of illumination, humidity is in 60-
80%;
4th step, acclimatization and transplantses;
Step S2~S4 condition of culture:Temperature is 15~25 DEG C, 1000~2000LUX of intensity of illumination, light application time 6~
12 hours/day;
Step S4 condition of culture:By the walnut plantlet of transplant for having two level root to garden mould+peat (2:1) in matrix, hide
Screened negative is sheltered from heat or light, and humidity 60-70%, goes out garden within 50~90 days.
Step S4 condition of culture is:Hardening matrix garden mould+peat quality proportioning (1.5~2.5):1, shade net is hidden
The moon, humidity 60-70%.
Embodiment two
The present embodiment and embodiment one are essentially identical, and difference is:
In step 1, by " branch survived after cuttage root-taking after the transplantation of seedlings of bulla walnut in big Tanaka plantation is material
Material, first after Bravo or Prochloraz sterilization, in quickly dipped appropriate plant growth regulator, then it is inserted in matrix and is trained
Support, rooted seedling is obtained after about 20~30 days ".