CN104823790B - A kind of bulla walnut cuttage fast seedling-cultivating method - Google Patents

A kind of bulla walnut cuttage fast seedling-cultivating method Download PDF

Info

Publication number
CN104823790B
CN104823790B CN201510214756.0A CN201510214756A CN104823790B CN 104823790 B CN104823790 B CN 104823790B CN 201510214756 A CN201510214756 A CN 201510214756A CN 104823790 B CN104823790 B CN 104823790B
Authority
CN
China
Prior art keywords
fringe bar
walnut
bulla
cuttage
otch
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201510214756.0A
Other languages
Chinese (zh)
Other versions
CN104823790A (en
Inventor
覃国乐
谢代祖
韦英明
韦坤华
韩俊严
韦艺
田永江
韦林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hechi Academy of forestry science and research
Original Assignee
Hechi Academy Of Forestry Science And Research
Hechi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hechi Academy Of Forestry Science And Research, Hechi University filed Critical Hechi Academy Of Forestry Science And Research
Priority to CN201510214756.0A priority Critical patent/CN104823790B/en
Publication of CN104823790A publication Critical patent/CN104823790A/en
Application granted granted Critical
Publication of CN104823790B publication Critical patent/CN104823790B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

A kind of bulla walnut cuttage fast seedling-cultivating method, comprises the following steps:(1) fringe bar is sheared;(2) fringe bar immersion and cuttage;(3) fringe bar vernalization is with taking root;(4) acclimatization and transplantses.The present invention greatly improves the bulla Juglans speed of growth and planting benefit, and cost is low, and emergence is fast, and technique is simpler, and Winter-Spring summer can produce, and is adapted to scale breeding.

Description

A kind of bulla walnut cuttage fast seedling-cultivating method
Technical field
The present invention relates to a kind of cultural method of bulla Juglans.
Background technology
Walnut (Juglans regia) is one of world-renowned four big dry fruits, is Juglandaceae juglans deciduous tree, in State's cultivation has the history of more than 2000, forms many kinds and type, and walnut is rich in fatty (more than 70%) and protein (20%) it is, high heat energy nutriment, is the green health care food of no cholesterol again, walnut is precious from head to foot, except walnut kernel Have outside edibility, its trunk, branch, leaf, rascal etc. have certain value.Walnut or rocky mountainous area Rocky Desertification Control The high seeds with the economic value of production of forestry.Walnut Industry be become mountain area resource inferior position into economic advantages, ecological dominance, The economic industry of the rocky mountainous area people is benefited, is a comprehensive benefit, the ecological systems engineering of long-acting benign cycle;It is One item collection economy, society, ecological triple effect are beneficial to integral integrated agriculture industry.However, walnut nursery is to suppress Walnut Industry Bottleneck, walnut Sexual breed seedling growth cycle length, about 10 or so years could as a result, it is difficult to solve plant dwarfing problem, it is sexual Reproduction characters of progenies segregation phenomenon is serious, and in improved seeds are cultivated and promoted, people are typically solved using grafting approach, and walnut Grafting be subject to seasonal restrictions again, survival rate is low, technical requirements are high, cost is big, production management difficulty is big, the tissue cultures of walnut are deposited In technical factors such as root inductions, take root undesirable, tissue culture is higher to technology, equipment requirement, and fund input amount is big, and obtain employment people The problems such as member's professional technique requires high, extremely difficult one of seeds that take root of Juglans, and common cuttage can not take root limits its application Promote.
At present, the growing and cultivation of bulla walnut mainly carries out nursery using traditional grafting and seedling from seed, raw Long period is grown, and floor space is big, it is difficult to realizes efficiently quickly heavy industrialization growing and cultivation.
The content of the invention
The technical problem to be solved in the present invention is, there is provided a kind of bulla Juglans cultural method, overcomes prior art difficult The defects of to realize efficiently quickly heavy industrialization growing and cultivation.
The technical solution adopted for the present invention to solve the technical problems is:A kind of bulla Juglans cultural method is provided, its It is characterised by, comprises the following steps:
S1, fringe bar shearing:To give birth to the healthy and strong branch of bulla walnut then as material, it is clipped after fringe bar stay 2-3 bud Point, upper end are cut flat, and lower end is smoothly cut into horse ear shape in the 45 degree of angles in place against axillary bud point, and otch can not be ftractureed, and 20-30 branch is bundled into A bundle, lower end is neat, standby;
S2, fringe bar immersion and cuttage:Fringe bar lower cut is after flowing water cleans 3~5 hours, the potassium permanganate through 0.1-1% Fringe bar sterilization is carried out, is transferred to after being soaked 6~12 hours in the solution containing appropriate plant growth regulator, what insertion had sterilized in advance Perlite+peat (3:1) in matrix;
The soaking solution includes plant growth regulator, potassium permanganate, and compound method is:Potassium permanganate is the sterilization of fringe bar Liquid, concentration 0.1-1%, appropriate plant growth regulator are formulated for 500-1500ppm;
S3, vernalization, culture of rootage:It is colonized 2 weeks posterior end otch and forms callus, winter is covered in Small plastic shed or greenhouse Heating or GA carry out vernalization to fringe bar, bud is produced itself growth hormone, hestening rooting, foliar fertilizer are sprayed after lamina, promote Photosynthesis, hestening rooting.15~25 DEG C of above-mentioned steps cultivation temperature, 1000~2000LUX of intensity of illumination, humidity is in 60- 80%;
S4, acclimatization and transplantses;
Step S2~S4 condition of culture:Temperature is 15~25 DEG C, 1000~2000LUX of intensity of illumination, light application time 6~ 12 hours/day;
Step S4 condition of culture:By the walnut plantlet of transplant for having two level root to garden mould+peat (2:1) in matrix, hide Screened negative is sheltered from heat or light, and humidity 60-70%, goes out garden within 50~90 days.
In the bulla Juglans cultural method of the present invention, the step S2, plant growth regulating that vernalization is taken root in S3 Agent is IBA sylvite and/or NAA, GA, the use of IBA-K concentration is 500-1500ppm, the use of NAA concentration is 100-500ppm, The use of GA concentration is 50-100ppm.
In the bulla Juglans cultural method of the present invention, the potassium permanganate concentration that is used in step S1 for 0.1%~ 1%.
In the bulla Juglans cultural method of the present invention, in step S3, about 2-8 centimetres of the length taken root, number of taking root is 10-20 bars.
In the bulla Juglans cultural method of the present invention, the cutting medium in step S3 is perlite+peat (3:1); Cultivation temperature is 20 ± 2 DEG C, 1000~2000LUX of intensity of illumination.
In the bulla Juglans cultural method of the present invention, the plant establishment conditioning agent is IBA-K and/or NAA, is used IBA-K concentration is 500-1500ppm, the use of NAA concentration is 100-500ppm.
The walnut vernalization conditioning agent GA, the use of GA concentration is 50- in the bulla Juglans cultural method of the present invention 100ppm。
Implement bulla Juglans cultural method of the present invention, compared with the prior art, it has the prominent advantages that:
The speed of growth and planting benefit greatly improve;The method bulla Juglans of the present invention start to take root for 40 days, average Rooting rate mean elements 10~20, there is 2 grades of root systems, can go out garden within 3 months up to 50~70%;And existing bulla walnut grafting The bud of method one can only be at most transferred on a stock, at most grow up to 1 young plant, growth cycle 1 year more than half;
Biennial seedling after cuttage root-taking, it can be taken root faster with Miao Fanmiao.
Introduces a collection can be made by the use of the branch cut every year as micro-material, reduce production cost, environmental pollution is small;
Technique is simpler, can produce throughout the year, is adapted to extensive nursery production.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment step:(1) fringe bar is sheared;(2) fringe bar immersion and cuttage;(3) with taking root, (4) hardening is moved for fringe bar vernalization Plant, survival rate can go out garden up to 70-80%, three months.
A kind of bulla Juglans cultural method of the present invention comprises the following steps:
The first step, fringe bar shearing:To give birth to the healthy and strong branch of bulla walnut then as material, it is clipped after fringe bar stay 2-3 Individual axillary bud point, upper end are cut flat, and lower end is cut into horse ear shape against 45 degree of angles at axillary bud point, and otch can not ftracture, 20-30 branch bundle Into a bundle, lower end is neat, standby;
Second step, fringe bar immersion:Fringe bar lower cut is after flowing water cleans 3~5 hours, the potassium permanganate through 0.1-1% enters Row fringe bar sterilizes, and is transferred to after being soaked 6~12 hours in the solution containing appropriate plant growth regulator, the treasure that insertion has sterilized in advance Zhu Yan+peat (3:1) in matrix;
The soaking solution includes plant growth regulator, potassium permanganate, and compound method is:Potassium permanganate is the sterilization of fringe bar Liquid, concentration 0.1-1%, appropriate plant growth regulator are formulated for 500-1500ppm;
Plant growth growth regulator IBA-K:3- butyric acid potassiums, it is plant endogenous auxin, cell division can be promoted With cell growth, the general entitled indole-3-butyric acid potassium plant cell cultur of English, molecule Formula is C12H12KNO2
Plant growth growth regulator NAA:1-Naphthaleneacetic acid, methyl α-naphthyl acetate, abbreviation NAA, it is a kind of Auximone.
GA:Gibberellin, basic structure are gibberellanes.It is a kind of plant hormone being widely present.
3rd step, vernalization, culture of rootage:It is colonized 2 weeks posterior end otch and forms callus, winter carries out overlay film to fringe bar Or heating or GA spray vernalization, bud is produced itself synthetic auxin, hestening rooting, foliar fertilizer are sprayed after lamina, promote Photosynthesis, hestening rooting, using the present invention 50 days after walnut rooting rate up to 50-70%, several 10-20 bars of taking root, root long 2-8 Centimetre, start have two level root.15~25 DEG C of above-mentioned steps cultivation temperature, 1000~2000LUX of intensity of illumination, humidity is in 60- 80%;
4th step, acclimatization and transplantses;
Step S2~S4 condition of culture:Temperature is 15~25 DEG C, 1000~2000LUX of intensity of illumination, light application time 6~ 12 hours/day;
Step S4 condition of culture:By the walnut plantlet of transplant for having two level root to garden mould+peat (2:1) in matrix, hide Screened negative is sheltered from heat or light, and humidity 60-70%, goes out garden within 50~90 days.
Step S4 condition of culture is:Hardening matrix garden mould+peat quality proportioning (1.5~2.5):1, shade net is hidden The moon, humidity 60-70%.
Embodiment two
The present embodiment and embodiment one are essentially identical, and difference is:
In step 1, by " branch survived after cuttage root-taking after the transplantation of seedlings of bulla walnut in big Tanaka plantation is material Material, first after Bravo or Prochloraz sterilization, in quickly dipped appropriate plant growth regulator, then it is inserted in matrix and is trained Support, rooted seedling is obtained after about 20~30 days ".

Claims (1)

1. a kind of bulla walnut cuttage fast seedling-cultivating method, it is characterised in that comprise the following steps:
(1), fringe bar is sheared:The healthy and strong branch for giving birth to bulla walnut then is cut into the fringe bar for staying 2-3 axillary bud, fringe bar upper end is cut Flat, lower end is cut into horse ear shape against 45 degree of angles at axillary bud, and otch can not be ftractureed, and 20-30 branch is bundled into a bundle, under hold level with both hands Together, it is standby;
(2), fringe bar immersion and cuttage:After the fringe bar otch that step (1) obtains is cleaned into 3~5 hours with flowing water, by fringe bar otch After 0.1-1% disinfecting solution of potassium permanganate, the sylvite concentration for being transferred to IBA is 500-1500ppm, and NAA concentration is 100- 500ppm, GA concentration are to be soaked 6~12 hours in 50-100ppm solution, are then inserted into sterilized perlite:Peat= 3:In 1 matrix, 15~25 DEG C, 1000~2000LUX of intensity of illumination of cultivation temperature is placed on, hour/day of light application time 6~12, Field planting culture is carried out in humidity 60-80% greenhouse;
(3), fringe bar vernalization, culture of rootage:Step (2) fringe bar is colonized 2 weeks posterior end otch and forms callus, and winter covering is small Heating or GA processing fringe bars carry out vernalization in shed or greenhouse, and with hestening rooting, foliar fertilizer is sprayed after lamina, promotes photosynthetic work With hestening rooting, 15~25 DEG C of cultivation temperature, 1000~2000LUX of intensity of illumination, humidity is in 60-80%;
(4), acclimatization and transplantses:The walnut plantlet of transplant of taking root that step (3) is obtained is to garden mould:Peat=2:In 1 matrix, shade net Shelter from heat or light, humidity is maintained at 60-70%, goes out garden within 50~90 days.
CN201510214756.0A 2015-04-29 2015-04-29 A kind of bulla walnut cuttage fast seedling-cultivating method Expired - Fee Related CN104823790B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510214756.0A CN104823790B (en) 2015-04-29 2015-04-29 A kind of bulla walnut cuttage fast seedling-cultivating method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510214756.0A CN104823790B (en) 2015-04-29 2015-04-29 A kind of bulla walnut cuttage fast seedling-cultivating method

Publications (2)

Publication Number Publication Date
CN104823790A CN104823790A (en) 2015-08-12
CN104823790B true CN104823790B (en) 2018-03-20

Family

ID=53802326

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510214756.0A Expired - Fee Related CN104823790B (en) 2015-04-29 2015-04-29 A kind of bulla walnut cuttage fast seedling-cultivating method

Country Status (1)

Country Link
CN (1) CN104823790B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105103930A (en) * 2015-09-30 2015-12-02 河池学院 Method for quickly cultivating murraya paniculata seedlings through cuttage
CN105103931A (en) * 2015-09-30 2015-12-02 河池学院 Brucea-javanica female-plant-cuttage rapid seedling growing method
CN106212175A (en) * 2016-07-31 2016-12-14 普定县绿源苗业开发有限公司 A kind of method of apocarya cutting propagation

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1706239A (en) * 2004-06-11 2005-12-14 中国林业科学研究院林业研究所 Walnut twig cutting propagation technology
CN102919033A (en) * 2012-11-17 2013-02-13 山西省农业科学院果树研究所 Method for processing cutting and rooting in tunnel matrix on twig tree of juglans hopeiensis Hu
CN103181284A (en) * 2013-01-21 2013-07-03 浙江农林大学 Hardwood cutting method for Carya illinoensis
CN103229691A (en) * 2012-12-31 2013-08-07 云南省林业技术推广总站 Constructing method for wide-narrow row J.sigillata Dode hard-twig cion cutting orchard
CN103238492A (en) * 2013-05-13 2013-08-14 江苏省中国科学院植物研究所 Process for facilitating sprouting of adventitious roots of apocarya branches
CN103299812A (en) * 2013-06-20 2013-09-18 南京绿宙薄壳山核桃科技有限公司 Apocarya hardwood cutting seedling raising method
CN103988697A (en) * 2014-05-30 2014-08-20 南京绿宙薄壳山核桃科技有限公司 Method for propagating apocarya seedlings
CN103999706A (en) * 2014-06-24 2014-08-27 南京林业大学 Method for increasing cutting rooting rate of pecans
CN104137755A (en) * 2014-07-25 2014-11-12 普定县持恒林业科技发展有限公司 Walnut seedling cultivating method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0242920A (en) * 1988-07-30 1990-02-13 Hiroki Sato Plant-breeding by grafting fig to walnuts

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1706239A (en) * 2004-06-11 2005-12-14 中国林业科学研究院林业研究所 Walnut twig cutting propagation technology
CN102919033A (en) * 2012-11-17 2013-02-13 山西省农业科学院果树研究所 Method for processing cutting and rooting in tunnel matrix on twig tree of juglans hopeiensis Hu
CN103229691A (en) * 2012-12-31 2013-08-07 云南省林业技术推广总站 Constructing method for wide-narrow row J.sigillata Dode hard-twig cion cutting orchard
CN103181284A (en) * 2013-01-21 2013-07-03 浙江农林大学 Hardwood cutting method for Carya illinoensis
CN103238492A (en) * 2013-05-13 2013-08-14 江苏省中国科学院植物研究所 Process for facilitating sprouting of adventitious roots of apocarya branches
CN103299812A (en) * 2013-06-20 2013-09-18 南京绿宙薄壳山核桃科技有限公司 Apocarya hardwood cutting seedling raising method
CN103988697A (en) * 2014-05-30 2014-08-20 南京绿宙薄壳山核桃科技有限公司 Method for propagating apocarya seedlings
CN103999706A (en) * 2014-06-24 2014-08-27 南京林业大学 Method for increasing cutting rooting rate of pecans
CN104137755A (en) * 2014-07-25 2014-11-12 普定县持恒林业科技发展有限公司 Walnut seedling cultivating method

Also Published As

Publication number Publication date
CN104823790A (en) 2015-08-12

Similar Documents

Publication Publication Date Title
CN102986445B (en) Process for cutting thick branches of golden camellia
CN102498905A (en) Method for quick cultivation of long-stem standard rose using cutting with long shoots
CN102187810B (en) Tissue culture propagation method for curcuma soloensis
CN104081954A (en) Hard wood cutting propagation method for paper mulberries
CN105075863B (en) A kind of Paeonia papaveracea rapid propagation method
CN101156551B (en) Dendrobium nobile stem segment cuttage method
CN104273037B (en) A kind of tissue culture and rapid propagation method of tail alpine ash DH33-27 kind
CN107018896B (en) A kind of method of facility cuttage tilia miqueliana
CN106034722A (en) Cutting propagation method of golden leaf plant namely weigela florida variegata
CN104285814B (en) A kind of tissue culture and rapid propagation method of tail alpine ash DH32-43 kind
CN102301896B (en) Arbored planting method of weigela floridas
CN104620921A (en) Rapid propagation method for jujubes
CN103190344A (en) Tissue culture method of fargesii
CN104365479B (en) A kind of tissue culture and rapid propagation method of tail alpine ash DH32-28 kind
CN104823790B (en) A kind of bulla walnut cuttage fast seedling-cultivating method
CN104285815B (en) A kind of tissue culture and rapid propagation method of tail alpine ash DH32-13 kind
CN103314748A (en) Seedling raising method of highbred honeysuckles
CN109006156A (en) A kind of grape fast seedling-cultivating method
CN104304028B (en) A kind of tissue culture and rapid propagation method of tail alpine ash DH32-26 kind
CN102657098A (en) In-vitro culture method for tender stem segments of cherry rootstock
CN109362524A (en) A kind of cultural method of African Chrysanthemum new varieties
CN105638482B (en) The method of walnut and Juglans mandshurica interspecific hybridization IMMATURE EMBRYOS CULTURE
CN104488679A (en) Acerpalmatum thunbf cuttage sapling cultivation method and formula
CN104529619A (en) Cultivating soil formula for rapid propagation of cuttage seedlings, and uses of cultivating soil
CN101766086B (en) Method for rapidly cutting and reproducing euphorbia pekinensis

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20171208

Address after: 546300 School of chemical and biological engineering, Hechi Universiry, Yizhou City, Hechi, the Guangxi Zhuang Autonomous Region, No. 42, Hechi Universiry

Applicant after: Hechi University

Applicant after: Hechi Academy of forestry science and research

Address before: 546300 School of chemical and biological engineering, Hechi Universiry, Yizhou City, Hechi, the Guangxi Zhuang Autonomous Region, No. 42, Hechi Universiry

Applicant before: Hechi University

GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180320

Termination date: 20190429