CN103238492A - Process for facilitating sprouting of adventitious roots of apocarya branches - Google Patents
Process for facilitating sprouting of adventitious roots of apocarya branches Download PDFInfo
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- CN103238492A CN103238492A CN2013101747510A CN201310174751A CN103238492A CN 103238492 A CN103238492 A CN 103238492A CN 2013101747510 A CN2013101747510 A CN 2013101747510A CN 201310174751 A CN201310174751 A CN 201310174751A CN 103238492 A CN103238492 A CN 103238492A
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Abstract
The invention discloses a process for facilitating sprouting of adventitious roots of apocarya branches. The method comprises the steps of selecting apocarya seedling branches of one year old, wherein diameters are within a range of 0.8-1.2cm, and the length is 12cm; laying electric heating lines at the bottom of a seedbed, then uniformly mixing four kinds of matrixes such as perlite, peat soil, river sand and vermiculite in proportion, and laying the mixture above electric heating lines, wherein the thickness is 10cm; immersing cut branches inside a rooting powder solution, and inserting branch morphological lower ends inside matrixes to be abut against electric heating lines, wherein the temperature difference between incision positions of cutting branch morphological lower ends and the outside temperature is within a range of 8-17 DEG C, and the water content of matrixes is controlled to be within a range of 25%-45%; conducting statistics for the rooting rate after 60 days, wherein the rooting rate is 59.26%. By the aid of the method, sprouting of adventitious roots of apocarya is effectively induced, so that the powerful technical support is provided for raising of seedlings of the apocarya by cutting.
Description
Technical field
The invention belongs to fruit tree and forest genetics field, relate to a kind of method that promotes that apocarya branch adventive root takes place particularly.
Background technology
Apocarya has another name called pecan tree, is a kind in Juglandaceae, the hickory.Originating in the U.S. and Mexico, is in the world one of important oil plant dry fruit tree variety, and the thin easy stripping of its shell, kernel plumpness, flavor are fragrant good to eat, have higher nutritive value.Perfectly straight, the tree-like grace of trunk, well established and vigorously developing is to turn the four sides green and the fine tree species of ornamental plantation.The tough and tensile densification of timber simultaneously, heartwood is dark red, sapwood is yellowish-white, full of elasticity, not yielding and cracking, is good commerical tree species such as building, noble furniture.Therefore, apocarya is one purposes is wide, the benefit period is long, economic benefit is high, social benefit and the tangible good economic tree of ecological benefits.
Apocarya was introduced China in 1900, the history in existing 113 years so far, though through several generations' unremitting effort, the still standing forest of none sheet standard and the orchard of a stable and high yields, let alone scale and industrialization at present.The roasted seeds and nuts " green fruit " that existing market is sold, " long-lived fruit " are all from U.S.'s import.This situation why occurs, many masters, odjective cause are arranged.Breeding of apocarya improved seeds is main by propagation by grafiting, and the used stock of propagation by grafiting mainly is from the root anvil, and the seedling cultivated of apocarya planting seed, owing to the expensive seedling cost that causes of seed is too high.1 kilogram of seed of apocarya about 100 ?160, germination rate is 60%, planting percent is 95%, every kilogram of seed planting percent be 52 ?91, so the seed costs price of every seedling is 1 yuan to 2 yuan.After the apocarya summer budding of life in other 1 year, will carry out stump behind the fallen leaves winter, and the annotinous branch behind the stump causes the wasting of resources without any value.If can solve the problem that apocarya seedling annotinous branch adventive root takes place, carry out cottage propagation, take full advantage of waste resource, reproduction coefficient will with 1:3 ?4 ratio be index year by year and increase, will reduce apocarya stock cost, solve the problem by the numerous seedling stock of grafting, effectively promote and promote the industrialization of apocarya.
Adventive root is that organs such as the stem, leaf of plant send, because of its adnation position indefinite, admittedly be called adventive root.The ability of regeneration adventive root is relevant with different plant species formed hereditary capacity in phylogenetic process.Because of the seeds difference, the difficulty or ease difference of regeneration adventive root, the ability of apocarya generation adventive root is very weak, there are some researches show that the ratio that the apocarya cuttage produces adventive root is too low, only is 4%.Therefore how improving apocarya adventive root incidence is this area problem anxious to be solved.
Summary of the invention
The objective of the invention is the above-mentioned defective at prior art, a kind of method that promotes that apocarya branch adventive root takes place is provided.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of method that promotes that apocarya branch adventive root takes place comprises the steps:
(1) branch is prepared:
Behind the annual apocarya seedling fallen leaves, the clip branch removes the tender taper branch in branch top, and cut the branch lower end, keep diameter 0.6 ?the branch of 1.5cm, be cut into length then and be 10 ?the brachyplast of 15cm;
(2) slotting machine is prepared:
Bottom shop, seedbed heating wire, then with perlite, peat soil, river sand, four kinds of matrix of vermiculite by the quality of 5～10:5～10:2～4:1 than mixing after, be layered on above the heating wire, thickness is 8 ?13cm;
(3) cuttage
With the branch form lower end (incision) that shears be immersed in concentration be 500 ?in the ABT rooting powder solution of 900mg/L 3 ?5h, then branch form lower end is inserted in the matrix, near heating wire, seeding row spacing be 5 ?15cm; (4) manage after the cuttage
The control of the temperature of branch form lower end (incision) and substrate base 23 ?25 ℃, ambient temperature control 8 ?15 ℃, the temperature difference that makes cuttage branch form lower end (incision) and external environment 8 ?17 ℃; Every 3 days water sprays once, make water content of substrate control 25% ?45%.
The present invention promotes in the method for apocarya branch adventive root generation that be annual February December to next year the period of cuttage.
The present invention promotes that the diameter of cuttage branch is preferably 1.0cm in the method for apocarya branch adventive root generation, and length is preferably 12cm.
The present invention promotes in the method for apocarya branch adventive root generation, perlite, peat soil, river sand, the preferred 8:8:3:1 of mass ratio of four kinds of matrix of vermiculite.
The present invention promotes that the thickness of medium of seedling bed is preferably 10cm in the method for apocarya branch adventive root generation.
The present invention promotes in the method for apocarya branch adventive root generation, the preferred flat nick of otch or the angular cut of cuttage branch form lower end.
The present invention promotes in the method for apocarya branch adventive root generation, preferred 25 ℃ of the temperature of branch form lower end cutting section (incision) and substrate base; Ambient temperature is preferably controlled at 9～11 ℃, further preferred 10 ℃; The temperature difference preferred 14 of cuttage branch form lower end (incision) and external environment ?16 ℃.
The present invention promotes that water content of substrate is preferably controlled 30% in the method for apocarya branch adventive root generation.
The present invention promotes in the method for apocarya branch adventive root generation, the preferred 600mg/L of the concentration of ABT root-inducing powder, and the branch form lower end that shears is immersed in the preferred 4h of time in the ABT solution.
The present invention promotes that the seeding row spacing of cuttage branch preferably is 10cm in the method for apocarya branch adventive root generation.
Beneficial effect:
At present, apocarya branch root induction rate is extremely low, the present invention utilizes ABT in conjunction with branch form lower end incision and the method that the ambient temperature difference combines in the apocarya seedling branch of life in 1 year, makes apocarya branch adventive root incidence reach 59.26%.Studies show that: having only ABT, do not having under the condition of the temperature difference, apocarya branch root induction rate is 0.Equally, having only the temperature difference, do not having under the condition of ABT, apocarya branch root induction rate also is 0.Therefore, in the process of apocarya branch root induction, two factors of ABT and the temperature difference (branch form lower end incision and ambient temperature are poor) are indispensable.In the East China, annual February December to next year, ambient temperature is about 8 ℃, so, as long as at substrate base floor file hot line, make the temperature control at branch form lower cut place about 25 ℃, strong environmental condition is created in the generation that namely can be apocarya branch adventive root, simple to operate, convenient and easy, be conducive to apocarya branch cutting breeding industrialization and carry out.
This invention can make the inductivity of 1 year living seedling branch adventive root of high apocarya reach 59.26%, has improved apocarya adventive root incidence to a great extent.Successful Application of the present invention, not only can take full advantage of waste resource, and by cottage propagation apocarya stock, reproduction coefficient will with 1:2 ?3 ratio be index year by year and increase, will solve apocarya by the problem of the numerous seedling stock of grafting quantity, effectively promote and promote the industrialization of apocarya.
Description of drawings
Fig. 1. the generation of apocarya adventive root
Embodiment
Embodiment 1
(1) experiment material
Behind the annual apocarya seedling fallen leaves, the clip branch removes the tender taper branch in branch top, keeps diameter at the branch of 1.0cm, is cut into the brachyplast that length is 12cm then.
(2) method of apocarya root induction
With the branch form lower end that shears be immersed in respectively that ABT concentration is 0,300,600,4h in the ABT solution of 900mg/L, carry out cuttage by following two kinds of methods then.In the experimentation, each handles 50 branches, repeats 3 times.
Method 1
Bottom shop, seedbed heating wire, then with perlite 40wt%, peat soil 40wt%, river sand 15wt%, four kinds of matrix of vermiculite 5wt% behind the mixing, are layered on above the heating wire in proportion, and thickness is 10cm.Then branch form lower end is inserted in the matrix, near heating wire, seeding row spacing is 10cm.Control about 25 ℃ in the temperature of branch form lower end cutting section and substrate base, substrate temperature reduces rapidly from the bottom to top, and ambient temperature control is (substrate temperature reduces rapidly from the bottom to top, and there are the temperature difference in substrate base and external environment) about 10 ℃.Every 3 days water sprays once, make water content of substrate control about 30%.
Method 2
Bottom shop, seedbed heating wire, then with perlite 40wt%, peat soil 40wt%, river sand 15wt%, four kinds of matrix of vermiculite 5wt% behind the mixing, are layered on above the heating wire in proportion, and thickness is 10cm.Then branch form lower end is inserted in the matrix, near heating wire, seeding row spacing is 10cm.The temperature of soil matrix and external environment is all controlled about 25 ℃ and (is not had the temperature difference).Every 3 days water sprays once, make water content of substrate control about 30%.
The result: under the condition that does not have root-inducing powder ABT and the temperature difference, apocarya branch root induction rate is 0; Having only root-inducing powder ABT, do not having under the condition of the temperature difference, apocarya branch root induction rate is 0; Equally, having only the temperature difference, do not having under the condition of root-inducing powder ABT, apocarya branch root induction rate also is 0.Therefore, in the process of apocarya branch root induction, two factors of root-inducing powder ABT and the temperature difference (branch form lower end incision and ambient temperature are poor) are indispensable.Having under the condition of the temperature difference, apocarya branch root induction rate is along with the increase of ABT concentration increases gradually, and when the concentration of ABT was 600mg/L, the inductivity maximum of adventive root reached 59.26, reduces subsequently.
Table 1 temperature difference and ABT concentration are to the influence of apocarya adventive root
Claims (10)
1. a method that promotes that apocarya branch adventive root takes place is characterized in that comprising the steps:
(1) branch is prepared:
Behind the annual apocarya seedling fallen leaves, the clip branch removes the tender taper branch in branch top, and cut the branch lower end, keep diameter 0.6 ?the branch of 1.5cm, be cut into length then and be 10 ?the brachyplast of 15cm;
(2) slotting machine is prepared:
Bottom shop, seedbed heating wire, then with perlite, peat soil, river sand, four kinds of matrix of vermiculite by the quality of 5～10:5～10:2～4:1 than mixing after, be layered on above the heating wire, thickness is 8 ?13cm;
(3) cuttage
With the branch form lower end that shears be immersed in concentration be 500 ?in the ABT rooting powder solution of 900mg/L 3 ?5h, then branch form lower end is inserted in the matrix, near heating wire, seeding row spacing be 5 ?15cm;
(4) manage after the cuttage
The control of the temperature of branch form lower end and substrate base 23 ?25 ℃, ambient temperature control 8 ?15 ℃, the temperature difference that makes cuttage branch form lower end and external environment 8 ?17 ℃; Every 3 days water sprays once, make water content of substrate control 25% ?45%.
2. method according to claim 1 is characterized in that December to the next year February of the period of cuttage for every year.
3. method according to claim 1, the diameter that it is characterized in that the cuttage branch is 1.0cm, length is 12cm.
4. method according to claim 1 is characterized in that perlite, peat soil, river sand, and the mass ratio of four kinds of matrix of vermiculite is 8:8:3:1.
5. method according to claim 1, the thickness that it is characterized in that medium of seedling bed is 10cm.
6. method according to claim 1 is characterized in that the otch of cuttage branch form lower end is flat nick or angular cut.
7. method according to claim 1, the temperature that it is characterized in that branch form lower end cutting section and substrate base is 25 ℃; Ambient temperature is controlled at 9～11 ℃, preferred 10 ℃; The temperature difference of cuttage branch form lower end and external environment 14 ?16 ℃.
8. method according to claim 1 is characterized in that water content of substrate control is 30%.
9. method according to claim 1 is characterized in that the concentration of ABT root-inducing powder is 600mg/L, and the time that the branch form lower end that shears is immersed in the ABT solution is 4h.
10. method according to claim 1 is characterized in that the seeding row spacing of cuttage branch is 10cm.
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Cited By (12)
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| CN103988697A (en) * | 2014-05-30 | 2014-08-20 | 南京绿宙薄壳山核桃科技有限公司 | Method for propagating apocarya seedlings |
| CN104081973A (en) * | 2014-06-21 | 2014-10-08 | 周口职业技术学院 | Experimental research method for promoting rooting of pomegranate |
| CN104137755A (en) * | 2014-07-25 | 2014-11-12 | 普定县持恒林业科技发展有限公司 | Walnut seedling cultivating method |
| CN104396656A (en) * | 2014-09-23 | 2015-03-11 | 常州市金土地农牧科技服务有限公司 | Yield-increasing method of pecans |
| CN104472199A (en) * | 2014-12-30 | 2015-04-01 | 广西壮族自治区药用植物园 | Cuttage propagation method for anisetree bark |
| CN104823790A (en) * | 2015-04-29 | 2015-08-12 | 河池学院 | Rapid juglans sigillata cutting propagation method |
| CN105052564A (en) * | 2015-08-05 | 2015-11-18 | 浙江农林大学 | Composite seedling method for cutting and grafting hard branches of carya illinoensis k. koch in corresponding period |
| CN107047262A (en) * | 2017-06-05 | 2017-08-18 | 四川格睿园林科技有限公司 | A kind of non-test tube seedling method of quick propagating and cultivating of Cotinus coggygria |
| CN107047180A (en) * | 2017-01-09 | 2017-08-18 | 江苏农牧科技职业学院 | A kind of apocarya epicormic branch cuttage method |
| CN107223506A (en) * | 2017-05-25 | 2017-10-03 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of thin skin hickory nut |
| CN108142192A (en) * | 2017-12-15 | 2018-06-12 | 合肥欣和园苗木有限公司 | Apocarya epicormic branch cuttage method |
| CN110383994A (en) * | 2019-07-30 | 2019-10-29 | 南京林业大学 | A kind of promotion method of apocarya breeding cuttage root-taking |
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Cited By (15)
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| CN103988697A (en) * | 2014-05-30 | 2014-08-20 | 南京绿宙薄壳山核桃科技有限公司 | Method for propagating apocarya seedlings |
| CN103988697B (en) * | 2014-05-30 | 2016-01-06 | 南京绿宙薄壳山核桃科技有限公司 | A kind of method of apocarya seedling breeding |
| CN104081973A (en) * | 2014-06-21 | 2014-10-08 | 周口职业技术学院 | Experimental research method for promoting rooting of pomegranate |
| CN104137755A (en) * | 2014-07-25 | 2014-11-12 | 普定县持恒林业科技发展有限公司 | Walnut seedling cultivating method |
| CN104396656A (en) * | 2014-09-23 | 2015-03-11 | 常州市金土地农牧科技服务有限公司 | Yield-increasing method of pecans |
| CN104472199A (en) * | 2014-12-30 | 2015-04-01 | 广西壮族自治区药用植物园 | Cuttage propagation method for anisetree bark |
| CN104472199B (en) * | 2014-12-30 | 2017-04-19 | 广西壮族自治区药用植物园 | Cuttage propagation method for anisetree bark |
| CN104823790B (en) * | 2015-04-29 | 2018-03-20 | 河池学院 | A kind of bulla walnut cuttage fast seedling-cultivating method |
| CN104823790A (en) * | 2015-04-29 | 2015-08-12 | 河池学院 | Rapid juglans sigillata cutting propagation method |
| CN105052564A (en) * | 2015-08-05 | 2015-11-18 | 浙江农林大学 | Composite seedling method for cutting and grafting hard branches of carya illinoensis k. koch in corresponding period |
| CN107047180A (en) * | 2017-01-09 | 2017-08-18 | 江苏农牧科技职业学院 | A kind of apocarya epicormic branch cuttage method |
| CN107223506A (en) * | 2017-05-25 | 2017-10-03 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of thin skin hickory nut |
| CN107047262A (en) * | 2017-06-05 | 2017-08-18 | 四川格睿园林科技有限公司 | A kind of non-test tube seedling method of quick propagating and cultivating of Cotinus coggygria |
| CN108142192A (en) * | 2017-12-15 | 2018-06-12 | 合肥欣和园苗木有限公司 | Apocarya epicormic branch cuttage method |
| CN110383994A (en) * | 2019-07-30 | 2019-10-29 | 南京林业大学 | A kind of promotion method of apocarya breeding cuttage root-taking |
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