CN104472199B - Cuttage propagation method for anisetree bark - Google Patents
Cuttage propagation method for anisetree bark Download PDFInfo
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- CN104472199B CN104472199B CN201410841544.0A CN201410841544A CN104472199B CN 104472199 B CN104472199 B CN 104472199B CN 201410841544 A CN201410841544 A CN 201410841544A CN 104472199 B CN104472199 B CN 104472199B
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- cutting
- seedbed
- cuttage
- cortex illicii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a cuttage propagation method for anisetree bark. The method comprises the following steps: a, selection and treatment of cutting slips: selecting robust branches, cutting into cutting slips, soaking the base parts of the cutting slips into disinfecting liquid for 30 minutes, and soaking in a rooting inductive agent for 2 hours; b, preparation of a substrate and a seedbed: selecting a uniform mixture of peat soil, perlite, vermiculite and fine river sand for serving as a cutting substrate, disinfecting the cutting substrate with disinfecting liquid, and making a seedbed of 15 centimeters in thickness; c, cottage, obliquely cutting the cutting slips into the seedbed substrate by 7-10 centimeters; d, management of the seedbed after cuttage: spraying water onto the seedbed in the morning and in the evening each day, spraying 500-time thiophanate methyl seven days later once every week for two times in total, spraying 3/4MS nutrient liquid one week later, and culturing till rooting and seedling emergence. By adopting the cuttage propagation method in breeding of anisetree bark seedlings, the breeding speed of the anisetree bark is increased, and the seedling breeding and scale planting of the anisetree bark are ensured greatly.
Description
Technical field
The present invention relates to a kind of cuttage breeding method of Cortex Illicii.
Background technology
Cortex Illicii (Illicium difengpi K.L.B.et K.I.M.) is Magnoliaceae anise platymiscium, also known as chases after ground
Wind, Cortex schizophragmatis integrifolii radiciss etc..Cortex Illicii is a kind of special product Chinese crude drug in EXPLOITATION AND UTILIZATION IN GUANGXI KARST, is used as medicine with bark, warm in nature, acrid in the mouth, puckery, tool
The effects such as having expelling wind and removing dampness, promoting the circulation of QI to relieve pain, has preferable curative effect to diseases such as Fall injuries, lumbar muscle strain and rheumatic arthritis,
It is the main raw material(s) of various Chinese patent medicine products.Cortex Illicii mainly originates in all peaces, Mashan, Debao to Longzhou of Southwestern Kwangsi, China etc.
Ground.
Cortex Illicii Regional Distribution is narrow and reserves are rare.With riseing year by year for market demand, the wild money of Cortex Illicii
Over-exploitation is suffered in source, is on the verge of exhaustion, lists in《Chinese Plants Red Data Book》Vulnerable species.Under natural conditions, Cortex Illicii mainly with
Seed is bred, but because of its kind of thin skin, grease deterioration when being dried is again perishable during overly moist, germinating power is easily lost, to science
Research and introducing and planting bring extreme difficulties.And cutting propagation can quickly breed in a short time substantial amounts of Cortex Illicii seedling, and
Also have the advantage for keeping maternal merit and low cost concurrently.Prior art is without for the report of Cortex Illicii cuttage breeding method
Road.
The content of the invention
The present invention has designed and developed a kind of cuttage breeding method of Cortex Illicii.The present invention breeds Cortex Illicii using cottage method
Seedling, to improve the reproduction speed of Cortex Illicii, the sapling multiplication and implantation in large scale for Cortex Illicii provides sound assurance.
The present invention provide technical scheme be：
A kind of cuttage breeding method of Cortex Illicii, the method is comprised the following steps：
A) selection of cutting and process：
A1) healthy and strong branch is selected, is cut into cutting；
A2) base portion of the cutting is soaked in disinfectant solution and is pulled out after 30min, dried in the air to surface do not drip in the cool；
A3) the cutting base portion is placed in be soaked in root induction agent and is pulled out after 2h, slightly dried in the air, drain surface water droplet；
B) preparation in substrate and seedbed：Cutting medium selects the uniform mixing of peat soil, perlite, Vermiculitum and thin river sand
Thing, makes the seedbed that thickness is 15cm after being carried out disinfection with disinfectant solution；
C) cuttage：The cutting is inclined into 40～50 ° of cuttages in the medium of seedling bed, depth is 7-10cm；
D) after cuttage seedbed management：Sooner or later 500 times of thiophanate methyl is sprayed to seedbed water spray after 7 days daily,
Spray weekly once, altogether spray twice, sprays 3/4MS nutritional solutions after one week, cultivate to taking root, emerge.
Preferably, in the cuttage breeding method of the Cortex Illicii, the cutting be 2-3 life, the thick 0.5-1cm of stem and
Band axillary bud.
Preferably, in the cuttage breeding method of the Cortex Illicii, the cutting length is 10-15cm, with 1～2
Tiltedly cut and clip angle at 45 ° individual axillary bud, upper end straight snips, lower end.
Preferably, in the cuttage breeding method of the Cortex Illicii, step a2) in disinfectant solution using 0.3%
Potassium permanganate solution.
Preferably, in the cuttage breeding method of the Cortex Illicii, step a3) in root induction agent into subpackage
Include the naphthalene acetic acid of 100-300mg/L, the gibberellins of 50-200mg/L, the indolebutyric acid of 200-800mg/L, the ABT of 10-50mg/L
No. 1 root-inducing powder, the chlorocholine chloride of 100-500mg/L and the vitamin C of 50-100mg/L.
Preferably, in the cuttage breeding method of the Cortex Illicii, peat soil in cutting medium in the step b) is precious
The volume ratio of Zhu Yan, Vermiculitum and thin river sand is 1: 1: 1: 1, and disinfectant solution adopts 1000 times of liquid of carbendazim.
Preferably, in the cuttage breeding method of the Cortex Illicii, cutting of the cuttage on seedbed in the step c)
Spacing in the rows is 6-8cm, and line-spacing is 10-12cm.
Preferably, in the cuttage breeding method of the Cortex Illicii, seedbed condition of culture is in the step d)：Temperature
23-28 DEG C, air humidity 70-80%, shade rate 50-60%.
Preferably, in the cuttage breeding method of the Cortex Illicii, the seedbed is first irrigated with water before cuttage, then is used
0.5% hydrogen peroxide solution is irrigated.
The present invention breeds Cortex Illicii seedling using cottage method, wherein the root induction agent using autogamy processes cutting, energy
The enzymatic activity of Cortex Illicii cutting cells in vivo is enough activated, promotes the schizogamy of cell, make root cell grow out faster,
So as to significantly improve the rooting rate of Cortex Illicii, and the present invention is environmentally friendly, easy to operate, while having the maternal Optimality of holding concurrently
The advantage of shape and low cost, with stronger practicality and promotional value.
Specific embodiment
With reference to embodiment, the invention will be further described, but should not be limited the scope of the invention with this.
Embodiment 1
2-3 lifes, the thick 0.5-1cm of stem and the stalwartness branch of the Cortex Illicii with axillary bud are selected, in the cool branch 10- is cut into into
15cm, the cutting with 1-2 axillary bud, cutting upper end straight snips, lower end is tiltedly cut, and clip is at 45 °, and otch is smooth without hair.With 0.3%
Potassium permanganate immersion cutting base portion 30min, pull out, dry in the air to surface do not drip in the cool.By through the cutting base portion of sterilization
It is placed in root induction agent (No. 1 root-inducing powder 10mg/ of naphthalene acetic acid 100mg/L, gibberellins 50mg/L, indolebutyric acid 200mg/L, ABT
L, chlorocholine chloride 100mg/L, vitamin C 50mg/L) middle immersion 2h, pull out, slightly dry in the air, drain surface water droplet.
By cutting medium peat soil, perlite, Vermiculitum, thin river sand 1: 1: 1: 1 mix homogeneously by volume, carbendazim is used
1000 times of liquid carry out disinfection, be made into thickness be 15cm seedbed, seedbed is first irrigated with water before cuttage, then with 0.5% dioxygen
Aqueous solution is irrigated.Then by cutting press spacing in the rows 6-8cm, line-spacing 10-12cm, incline 40～50 ° of cuttages in medium of seedling bed, depth
For 7-10cm.Period, spray water sooner or later, holding seedbed condition of culture is：Temperature 23-28 DEG C, air humidity 70-80%, shade rate
50-60%.500 times of thiophanate methyl is sprayed after 7 days, is sprayed weekly once, spray altogether twice, sprays 3/4MS nutritional solutions after one week.
After cuttage 40d, disposable lifting investigation rooting percent is 85.7%.
Embodiment 2
2-3 lifes, the thick 0.5-1cm of stem and the stalwartness branch of the Cortex Illicii with axillary bud are selected, in the cool branch 10- is cut into into
15cm, the cutting with 1-2 axillary bud, cutting upper end straight snips, lower end is tiltedly cut, and clip is at 45 °, and otch is smooth without hair.With 0.3%
Potassium permanganate immersion cutting base portion 30min, pull out, dry in the air to surface do not drip in the cool.By through the cutting base portion of sterilization
It is placed in root induction agent (No. 1 root-inducing powder 25mg/ of naphthalene acetic acid 200mg/L, gibberellins 100mg/L, indolebutyric acid 400mg/L, ABT
L, chlorocholine chloride 250mg/L, vitamin C 75mg/L) soak time be 2h, pull out, slightly dry in the air, drain surface water droplet.
By cutting medium peat soil, perlite, Vermiculitum, thin river sand 1: 1: 1: 1 mix homogeneously by volume, carbendazim is used
1000 times of liquid carry out disinfection, be made into thickness be 15cm seedbed, seedbed is first irrigated with water before cuttage, then with 0.5% dioxygen
Aqueous solution is irrigated.Then by cutting press spacing in the rows 6-8cm, line-spacing 10-12cm, incline 40～50 ° of cuttages in medium of seedling bed, depth
For 7-10cm.Period, spray water sooner or later, holding seedbed condition of culture is：Temperature 23-28 DEG C, air humidity 70-80%, shade rate
50-60%.500 times of thiophanate methyl is sprayed after 7 days, is sprayed weekly once, spray altogether twice, sprays 3/4MS nutritional solutions after one week.
Disposable lifting investigation rooting percent is 94% after cuttage 40d.
Embodiment 3
2-3 lifes, the thick 0.5-1cm of stem and the stalwartness branch of the Cortex Illicii with axillary bud are selected, in the cool branch 10- is cut into into
15cm, the cutting with 1-2 axillary bud, cutting upper end straight snips, lower end is tiltedly cut, and clip is at 45 °, and otch is smooth without hair.With 0.3%
Potassium permanganate immersion cutting base portion 30min, pull out, dry in the air to surface do not drip in the cool.By through the cutting base portion of sterilization
It is placed in root induction agent (No. 1 root-inducing powder 50mg/ of naphthalene acetic acid 300mg/L, gibberellins 200mg/L, indolebutyric acid 800mg/L, ABT
L, chlorocholine chloride 500mg/L, vitamin C 100mg/L) soak time be 2h, pull out, slightly dry in the air, drain surface water droplet.
By cutting medium peat soil, perlite, Vermiculitum, thin river sand 1: 1: 1: 1 mix homogeneously by volume, carbendazim is used
1000 times of liquid carry out disinfection, be made into thickness be 15cm seedbed, seedbed is first irrigated with water before cuttage, then with 0.5% dioxygen
Aqueous solution is irrigated.Then by cutting press spacing in the rows 6-8cm, line-spacing 10-12cm, incline 40～50 ° of cuttages in medium of seedling bed, depth
For 7-10cm.Period, spray water sooner or later, holding seedbed condition of culture is：Temperature 23-28 DEG C, air humidity 70-80%, shade rate
50-60%.500 times of thiophanate methyl is sprayed after 7 days, is sprayed weekly once, spray altogether twice, sprays 3/4MS nutritional solutions after one week.
Disposable lifting investigation rooting percent is 92.8% after 40d.
In sum, in promoting the method for Cortex Illicii cutting propagation, preferred plan is that Cortex Illicii cutting is placed in into autogamy
Root induction agent (No. 1 root-inducing powder 25mg/L of naphthalene acetic acid 200mg/L, gibberellins 100mg/L, indolebutyric acid 400mg/L, ABT, it is short
Strong element 250mg/L, vitamin C 75mg/L) 2h is soaked in solution, then in peat soil, perlite, Vermiculitum, thin river sand=1: 1:
Cultivate in the substrate of 1: 1 (volume ratio).The Cortex Illicii rooting percent that the program is obtained is high, and reproduction speed is fast, and seedling is healthy and strong.
Although the embodiment of invention is disclosed as above, it is not restricted to listed fortune in description and embodiment
With, it can be applied to completely various suitable the field of the invention, for those skilled in the art, can be easily real
Now other modification, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited to
Specific details and shown here as the proportioning example with description.
Claims (6)
1. a kind of cuttage breeding method of Cortex Illicii, it is characterised in that the method is comprised the following steps：
A) selection of cutting and process：
A1) healthy and strong branch is selected, is cut into cutting；
A2) base portion of the cutting is soaked in disinfectant solution and is pulled out after 30min, dried in the air to surface do not drip in the cool, it is described
Disinfectant solution adopts 0.3% potassium permanganate solution；
A3) the cutting base portion is placed in be soaked in root induction agent and is pulled out after 2h, slightly dried in the air, drain surface water droplet, it is described to take root
The naphthalene acetic acid of the composition of derivant including 100-300mg/L, the gibberellins of 50-200mg/L, the indolebutyric acid of 200-800mg/L,
The ABT1 root-inducing powders of 10-50mg/L, the chlorocholine chloride of 100-500mg/L and the vitamin C of 50-100mg/L；
B) preparation in substrate and seedbed：Cutting medium selects the homogeneous mixture of peat soil, perlite, Vermiculitum and thin river sand, uses
Disinfectant solution makes the seedbed that thickness is 15cm, peat soil in the cutting medium, perlite, Vermiculitum and thin river sand after carrying out disinfection
Volume ratio be 1:1:1:1, disinfectant solution adopts 1000 times of liquid of carbendazim；
C) cuttage：The cutting is inclined into 40-50 ° of cuttage in the medium of seedling bed, depth is 7-10cm；
D) after cuttage seedbed management：Sooner or later 500 times of thiophanate methyl is sprayed, weekly to seedbed water spray after 7 days daily
Once, altogether spray twice, sprays 3/4MS nutritional solutions after one week for spray, cultivates to taking root, emerge.
2. the cuttage breeding method of Cortex Illicii as claimed in claim 1, it is characterised in that the cutting is that 2-3 lifes, stem are thick
0.5-1cm and band axillary bud.
3. the cuttage breeding method of Cortex Illicii as claimed in claim 2, it is characterised in that the cutting length is 10-15cm,
With 1～2 axillary bud, upper end straight snips, lower end is tiltedly cut and clip angle at 45 °.
4. the cuttage breeding method of Cortex Illicii as claimed in claim 1, it is characterised in that cuttage is in seedbed in the step d)
On cutting spacing in the rows be 6-8cm, line-spacing is 10-12cm.
5. the cuttage breeding method of Cortex Illicii as claimed in claim 1, it is characterised in that culture bar in seedbed in the step d)
Part is：Temperature 23-28 DEG C, air humidity 70-80%, shade rate 50-60%.
6. the cuttage breeding method of Cortex Illicii as claimed in claim 1, it is characterised in that water is first used before cuttage in the seedbed
Irrigate, then irrigated with 0.5% hydrogen peroxide solution.
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| CN201410841544.0A CN104472199B (en) | 2014-12-30 | 2014-12-30 | Cuttage propagation method for anisetree bark |
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| CN201410841544.0A CN104472199B (en) | 2014-12-30 | 2014-12-30 | Cuttage propagation method for anisetree bark |
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| CN104472199B true CN104472199B (en) | 2017-04-19 |
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| CN105145071A (en) * | 2015-09-06 | 2015-12-16 | 广西壮族自治区药用植物园 | Cutting propagation method of brucea javanica |
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| CN114303816B (en) * | 2021-12-30 | 2022-11-29 | 广西壮族自治区中国科学院广西植物研究所 | Conservation method for karst endangered plant anisetree bark |
| CN115997566A (en) * | 2022-12-22 | 2023-04-25 | 延边大学 | Rapid cutting propagation method for rhodiola sachalinensis |
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