CN102668864A - Method for performing cuttage propagation on Torreya yunnanenssi Cheng et L..K.Fu - Google Patents
Method for performing cuttage propagation on Torreya yunnanenssi Cheng et L..K.Fu Download PDFInfo
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Abstract
The invention discloses a method for performing cuttage propagation on Torreya yunnanenssi Cheng et L.K.Fu, belongs to the technical field of plant cultivation, and in particular relates to a method for performing cuttage propagation on Torreya yunnanenssi Cheng et L.K.Fu. The method is characterized by comprising the following steps of: (1) preparing a cutting wood: taking an annual-grown strong branch of which the length is between 8 and 12cm as the cutting wood; (2) putting the cutting wood into mixed liquid of naphthalene acetic acid aqueous solution of 1,000mg/L and chlorothalonil of 2,000mg/L for soaking; (3) paving a brick seedbed with the height of 20cm in a greenhouse tunnel, and paving a sand bed or a matrix bed on the brick seedbed; (4) inserting the cutting wood into the sand bed or the matrix bed; (5) carrying out daily management; and (6) exercising seedling and changing a garden: cultivating a cutting wood seedling root in the open air by changing the small greenhouse tunnel after the cutting wood seedling root grows out of the soil. With the adoption of the method, the problem that the Torreya yunnanenssi Cheng et L.K.Fu difficultly takes root is solved, and the method is simple and feasible and low in cost, so that the blank for performing cuttage propagation on Torreya yunnanenssi Cheng et L.K.Fu for culturing seedlings in a scale mode can be made up.
Description
Technical field
The invention belongs to field of plant growing technology, be specially the Semen seu cortex Torreyae yunnanensis cuttage breeding method.
Background technology
Semen seu cortex Torreyae yunnanensis (
Torreya yunnanenssiCheng et L.K.Fu); The taxaceae aiphyllium; Be distributed in the northwestern Yunnan Province area, scattered in 2000 to 3400 meters valley floors of height above sea level or hillside softwood forest, theropencedrymion, be China's endemic tree; For the most western kind of Torreya distribution, the research Chinese torreya is belonged to classification, is distributed with important academic significance.This tree lumber is solid, and texture is careful and even, does not ftracture, and water-fast wet, rotproofness is strong, for the high-quality of making house, bridge, furniture, utensil, farm implements is used material.Its fruit is pharmaceutically acceptable, treatment infantile malnutrition, abdominal pain due to enterositosis, and diseases such as constipation and hemorrhoid, also can extract oil supplies industry practical.In addition, the Semen seu cortex Torreyae yunnanensis tree crown is neat, the strong luxuriantly Cheng Yin of branches and leaves, and contamination resistance is stronger, is outstanding green tree species.
Semen seu cortex Torreyae yunnanensis is a Chinese Second Class Key Protected Plant, belongs to the species of gradually endangering.These seeds are dioecian plants, male and female plant skewness under wild environment, and pollination is difficult, causes plant rare; The progeny size that grows directly from seeds is very limited, and ripening rate is low in addition, and the branch of biennial bearing is arranged; Normal 2-3 is once solid, and fruit often suffers muroid to nibble food again, so upgrade bad naturally.Owing to receive the interference of human factor, Semen seu cortex Torreyae yunnanensis nature population quantity sharply reduces in recent years.
Semen seu cortex Torreyae yunnanensis is the dominant tree species in the forest community on ground such as NW YUNNAN Wei Xi county, can be used as local good reproducting tree species, but seedling rare be to hinder the matter of utmost importance that it enlarges plantation.The Semen seu cortex Torreyae yunnanensis of the sexual propagation offspring plant of growing directly from seeds is uneven; Quality discrepancy is very big between plant; Excellent genotypic merit can not stably pass to the offspring through sexual propagation; Thereby significantly impacts Semen seu cortex Torreyae yunnanensis timber and plant real quality, and the local masses' fragmentary cuttage test shows that its cuttage seeding is difficult to take root that existing reproduction speed can not satisfy the seedling demand far away.Asexual cottage propagation is to solve the effective way that Semen seu cortex Torreyae yunnanensis expands numerous production.
Summary of the invention
Can not keep the breeding characteristic in order to solve Semen seu cortex Torreyae yunnanensis sexual propagation, and the take root problem of difficulty of existing cuttage technique, the present invention proposes a kind of Semen seu cortex Torreyae yunnanensis cuttage breeding method.
Semen seu cortex Torreyae yunnanensis cuttage breeding method of the present invention is characterized in that this method realizes through following step:
1) inserting fringe prepares: get length and insert fringe in the annual healthy and strong branch conduct of 8-12cm;
2) will insert fringe puts into the mixed liquor of tpn of the methyl aqueous solution and the 2000mg/L of 1000mg/L and soaks;
3) husky bed or matrix bed are laid in the brick seedbed of the high 20cm of laying in green house on the brick seedbed;
4) fringe be will insert and husky bed or matrix bed inserted;
5) daily management cuttage temperature is controlled at 20-25 ℃, and humidity is controlled at 60%-85%, weekly cuttage seeding is sprayed tpn 800-1000 times liquid or carbendazim 600-800 times liquid once, control illumination by the low light level that shades according to excessive to natural lighting gradually;
6) refining seedling changes the garden: insert and to remove Xiao Wenpeng after the fringe shoot root grows and change the garden and cultivate in the open.
Described slotting fringe needs wipe out the blade of inserting the nearly stem end 1/3 of fringe from base portion, and it is intact to keep inserting the fringe bark, will insert the ear stem end and be soaked in downwards in the clear water 1-2 hour, inserts the fringe preservation to play.
The mixed liquor soak time of tpn that described slotting fringe is put into the methyl aqueous solution and the 2000mg/L of 1000mg/L is 30-60 minute, will insert fringe then and take out that to place shady and cool place subsequent use after 30 minutes, to guarantee that inserting fringe can fully be acted on medicament.
Described brick seedbed specifically is the brick seedbed of in green house, laying high 20cm; Lay husky bed or matrix bed on the brick seedbed, the thin river sand of thick 8-10cm is adopted on husky bed upper strata, middle level tiling nonwoven; It is the little gravel of 2cm that thickness is placed by lower floor; The thick 8-10cm of matrix bed, by perlite, peat, vermiculite by volume the ratio of 10:30:1 form, on the seedbed, build Xiao Wenpeng again after lay accomplishing.
Described slotting fringe is when inserting husky bed or matrix bed, and cutting time is chosen in afternoon or at dusk, the cuttage degree of depth is husky bed 5-6cm; Matrix 3-5cm, face of blade is to the south, and cutting becomes 75 ° of angles with bed surface matrix; Water will irrigate after the cuttage, and with 1000 times of liquid sprayings of carbendazim, little temperature canopy is obturaged.
Described refining seedling; Changing the garden is to grow to 1-1.5cm refining seedling at slotting fringe shoot root; Remove Xiao Wenpeng after 7 days, change the garden after 30-50 days, with intensive cultivation fine earth and humus soil by volume 1:10 puddle evenly as the nutritious bag cultivation matrix; Transplant sunshade 75% in preceding 30 days, when slotting fringe height of seedling reaches 15-30cm, just can cultivate in the open.
Wherein, the methyl aqueous solution is a plant growth regulators, and tpn is a kind of bactericidal agent, and inserting fringe on the one hand can be to inserting the fringe sterilization to prevent that inserting fringe infects after these two kinds of solution soaking, on the other hand with promote to insert fringe growth.Husky bed or matrix bed all have good water penetration, can promote cuttage rooting.
The invention solves the problem that the Semen seu cortex Torreyae yunnanensis cuttage is difficult for taking root; Method is simple, cost is low; Filled up the scale Semen seu cortex Torreyae yunnanensis cottage propagation blank of growing seedlings; Can realize effective fast asexual propagation of Semen seu cortex Torreyae yunnanensis, promote, promote that to accelerating Semen seu cortex Torreyae yunnanensis excellent genes type its child care and afforestation are promoted and the reasonable resources utilization has very important realistic meaning.
Embodiment
Embodiment 1:2011 October in Yunnan Province Wei Xixian adopt respectively good staminiferous plant and the female plant branch of proterties, cuttage, refining seedling, cultivation are all accomplished in practice base, southwestern Forestry University gardens.
Insert the selection of fringe: annual Semen seu cortex Torreyae yunnanensis branch is about 8m as inserting fringe on the selection plant in young, middle age, and branch is healthy and strong, and blade is emerald green, no damage by disease and insect.
Insert the preservation of fringe: inserting fringe fresh-keeping is the key of cutting survival, and leaving shady and cool lucifuge with the clear water immersion in after 10 minutes after gathering does not have in the wind environment, in 48 hours, accomplishes cuttage.
Insert the mechanical treatment of fringe: the Semen seu cortex Torreyae yunnanensis petiole is short; Hold 2/3 blade slightly in the cuttage reservation, nearly stem end 1/3 blade removes with hand or scissors under it, notices during excision that the branch cortex remains intact; Insert about 20 of fringes and tie up with elastic, base portion is put into clear water downwards and was soaked 1 hour.
To insert the fringe base portion in the mixed liquor of the methyl aqueous solution of chemical treatment: 1000mg/L and the tpn of 2000mg/L soaks downwards to take out after 30 minutes in solution and is placed on shady and cool place 30 minutes in order to cuttage.
The arrangement in seedbed is prepared: in green house, build the brick seedbed of high 20cm, and the husky bed of last layings, the thickness of sand bed is 8cm, husky bedside 1m, sand is transferred nonwoven, and nonwoven is transferred the gravel of about 2cm behind one deck, is beneficial to draining.On the seedbed, cover the little temperature canopy of formation with thin bamboo strips used for weaving and Polypropylence Sheet.
Cuttage: will insert the fringe cuttage on husky bed, cutting time is chosen at dusk, and no strong illumination helps the recovery of branch, and the cuttage degree of depth is 5-6cm, and face of blade faces south, and cutting becomes 75 ° of angles with bed surface matrix; After the cuttage for the first time water to irrigate, and, little temperature canopy is obturaged, to prevent the disease generation with 1000 times of liquid sprayings of carbendazim.
Daily management: cuttage initial stage temperature is controlled at 20 ℃, and humidity remains on 70%, and blade was prone to come off when humidity was excessive; Use 800 times of liquid of tpn to spray weekly and once prevent disease, notice that the little greenhouse of control temp. and humidity changes, fine two to three coolings of atomized water spray outside Xiao Wenpeng prevent that temperature from raising, and humidity remains on 60%.Temperature progressively raises from low to high after the cuttage, and the regulation and control of temperature help the evaporation and the absorption equilibrium of the slotting fringe moisture of firm cuttage, and the later stage day temperature is controlled at 25 ℃, humidity 60%; Night, temperature was controlled at 20 ℃, and humidity 80% is observed slotting fringe callus and formed suitably reduction water supply in media of back, and illumination grows from weak to strong.
The refining seedling, change the garden: shoot root reaches 1cm, after begin to ventilate and increase gradually the ventilation duration, method is the sealing on daytime, opens little Wen Peng two ends night, seedling removes Xiao Wenpeng after adapting to external environment condition after 7 days, gravity-flow ventilation, matrix is gradually done to cultivate after 30 days and is changed the garden.When changing the garden with intensive cultivation fine earth and humus soil by volume the ratio mixing of 1:10 put into nutritious bag, be chosen at dusk the time-division to move in the bag and cultivate, moving in back 30 days must sunshade 75%, sprays water in good time and keeps matrix moistening, height of seedling reaches 15-30cm and can cultivate in the open.
Embodiment 2:2011 October in Yunnan Province Wei Xixian adopt respectively good staminiferous plant and the female plant branch of proterties, cuttage, refining seedling, cultivation are all accomplished in practice base, southwestern Forestry University gardens.
Insert the selection of fringe: select children, middle plant annotinous branch conduct in age to insert fringe, be about 12cm, branch is healthy and strong, and blade is emerald green, no damage by disease and insect.
Insert the preservation of fringe: inserting the fresh-keeping of fringe is the key of cutting survival, and leaving shady and cool lucifuge with the clear water immersion in after 10 minutes after gathering does not have in the wind environment, in 48 hours, accomplishes cuttage.
Insert the mechanical treatment of fringe: the Semen seu cortex Torreyae yunnanensis petiole is short; Keep in cuttage and to hold 2/3 blade slightly, its down nearly stem end 1/3 blade remove with hand or scissors, excision the time notice that the branch cortex remains intact; About 20 of fringe bars are tied up with elastic, and base portion is put into clear water downwards and soaked 2 hours.
To insert the fringe base portion in the mixed liquor of the methyl aqueous solution of chemical treatment: 1000mg/L and the tpn of 2000mg/L soaks downwards to take out after 30 minutes in solution and is placed on shady and cool place 30 minutes in order to cuttage.
The arrangement in seedbed is prepared: build the brick seedbed of high 20cm at green house, last laying is by perlite, peat, and vermiculite is the matrix bed of 10:30:1 composition by volume, and the about 10cm of matrix bed thickness waters permeable; On the seedbed, cover two layers of greenhouse of formation with thin bamboo strips used for weaving and Polypropylence Sheet.
Cuttage: will insert the fringe cuttage on the matrix bed, cutting time is chosen at dusk, and no strong illumination helps the recovery of branch, and the cuttage degree of depth is 6cm, and face of blade faces south, and cutting becomes 75 ° of angles with bed surface matrix.After the cuttage for the first time water to irrigate, and, little temperature canopy is obturaged, to prevent the disease generation with 1000 times of liquid sprayings of carbendazim.
Daily management: cuttage initial stage temperature is controlled at 20 ℃, and humidity remains on 85%, uses 800 times of liquid of carbendazim to spray weekly and once prevents disease; Notice that the little greenhouse of control temp. and humidity changes; Fine two to three coolings of atomized water spray outside Xiao Wenpeng prevent that temperature from raising, and humidity remains on 60%.Temperature progressively raises from low to high after the cuttage, and the regulation and control of temperature help the evaporation and the absorption equilibrium of the slotting fringe moisture of firm cuttage, and the later stage day temperature is controlled at 25 ℃, humidity 60%; Night, temperature was controlled at 20 ℃, and humidity 80% is observed slotting fringe callus and formed suitably reduction water supply in media of back, and illumination grows from weak to strong.
The refining seedling, change the garden: shoot root reaches 1.5cm, after begin to ventilate and increase gradually the ventilation duration, method is the sealing on daytime, opens little Wen Peng two ends night, seedling removes Xiao Wenpeng after adapting to external environment condition after 7 days, gravity-flow ventilation, matrix is gradually done to cultivate after 50 days and is changed the garden.When changing the garden with intensive cultivation fine earth and humus soil by volume the ratio mixing of 1:10 put into nutritious bag, be chosen at dusk the time-division to move in the bag and cultivate, moving in back 30 days must sunshade 75%, sprays water in good time and keeps matrix moistening, height of seedling reaches 30cm and can cultivate in the open.
Claims (2)
1. Semen seu cortex Torreyae yunnanensis cuttage breeding method is characterized in that this method realizes through following step:
1) inserting fringe prepares: get length and insert fringe in the annual healthy and strong branch conduct of 8-12cm;
2) will insert fringe puts into the mixed liquor of tpn of the methyl aqueous solution and the 2000mg/L of 1000mg/L and soaks;
3) husky bed or matrix bed are laid in the brick seedbed of the high 20cm of laying in green house on the brick seedbed;
4) fringe be will insert and husky bed or matrix bed inserted;
5) daily management cuttage temperature is controlled at 20-25 ℃, and humidity is controlled at 60%-85%, weekly cuttage seeding is sprayed tpn 800-1000 times liquid or carbendazim 600-800 times liquid once, control illumination by the low light level that shades according to excessive to natural lighting gradually;
6) refining seedling changes the garden: insert and to remove Xiao Wenpeng after the fringe shoot root grows and change the garden and cultivate in the open.
2. a kind of Semen seu cortex Torreyae yunnanensis cuttage breeding method as claimed in claim 1 is characterized in that:
Described slotting fringe needs wipe out the blade of inserting the nearly stem end 1/3 of fringe from base portion, and it is intact to keep inserting the fringe bark, will insert the ear stem end and be soaked in downwards in the clear water 1-2 hour, inserts the fringe preservation to play;
The mixed liquor soak time of tpn that described slotting fringe is put into the methyl aqueous solution and the 2000mg/L of 1000mg/L is 30-60 minute, will insert fringe then and take out that to place shady and cool place subsequent use after 30 minutes, to guarantee that inserting fringe can fully be acted on medicament;
Described brick seedbed specifically is the brick seedbed of in green house, laying high 20cm; Lay husky bed or matrix bed on the brick seedbed, the thin river sand of thick 8-10cm is adopted on husky bed upper strata, middle level tiling nonwoven; It is the little gravel of 2cm that thickness is placed by lower floor; The thick 8-10cm of matrix bed, by perlite, peat, vermiculite by volume the ratio of 10:30:1 form, on the seedbed, build Xiao Wenpeng again after lay accomplishing;
Described slotting fringe is when inserting husky bed or matrix bed, and cutting time is chosen in afternoon or at dusk, the cuttage degree of depth is husky bed 5-6cm; Matrix 3-5cm, face of blade is to the south, and cutting becomes 75 ° of angles with bed surface matrix; Water will irrigate after the cuttage, and with 1000 times of liquid sprayings of carbendazim, little temperature canopy is obturaged;
Described refining seedling; Changing the garden is to grow to 1-1.5cm refining seedling at slotting fringe shoot root; Remove Xiao Wenpeng after 7 days, change the garden after 30-50 days, with intensive cultivation fine earth and humus soil by volume 1:10 puddle evenly as the nutritious bag cultivation matrix; Transplant sunshade 75% in preceding 30 days, when slotting fringe height of seedling reaches 15-30cm, just can cultivate in the open.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238448A (en) * | 2013-05-27 | 2013-08-14 | 江苏省中国科学院植物研究所 | Cutting propagation method of torreya jackii chun |
| CN104012334A (en) * | 2013-03-01 | 2014-09-03 | 江苏省中国科学院植物研究所 | Slotting machine facility construction method for high-efficiency breeding of Ascendens mucronatum series varieties |
| CN104472199A (en) * | 2014-12-30 | 2015-04-01 | 广西壮族自治区药用植物园 | Cuttage propagation method for anisetree bark |
| CN104838829A (en) * | 2014-02-14 | 2015-08-19 | 周欣 | Asexual rapid propagation method for high quality edible Chinese torreya |
| CN105875146A (en) * | 2016-04-18 | 2016-08-24 | 杭州富阳飞博科技有限公司 | Vegetative propagation method of torreya jackii |
| CN106386336A (en) * | 2016-08-30 | 2017-02-15 | 四川七彩林业开发有限公司 | Pyracantha fortuneana Harlequin cuttage breeding method |
| CN106416680A (en) * | 2016-09-08 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Melaleuca alternifolia cutting propagation method |
| CN108887162A (en) * | 2018-06-26 | 2018-11-27 | 赤水市信天中药产业开发有限公司 | A kind of HERBA DENDROBII cuttage breeding method |
| CN115053903A (en) * | 2022-06-27 | 2022-09-16 | 菏泽市农业科学院(山东省农业科学院菏泽市分院) | Peony root soaking solution and peony seedling raising method |
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| CN104012334A (en) * | 2013-03-01 | 2014-09-03 | 江苏省中国科学院植物研究所 | Slotting machine facility construction method for high-efficiency breeding of Ascendens mucronatum series varieties |
| CN104012334B (en) * | 2013-03-01 | 2016-09-28 | 江苏省中国科学院植物研究所 | The slotting machine facility construction method that a kind of middle mountain China fir series of products is efficiently bred |
| CN103238448A (en) * | 2013-05-27 | 2013-08-14 | 江苏省中国科学院植物研究所 | Cutting propagation method of torreya jackii chun |
| CN104838829A (en) * | 2014-02-14 | 2015-08-19 | 周欣 | Asexual rapid propagation method for high quality edible Chinese torreya |
| CN104472199A (en) * | 2014-12-30 | 2015-04-01 | 广西壮族自治区药用植物园 | Cuttage propagation method for anisetree bark |
| CN104472199B (en) * | 2014-12-30 | 2017-04-19 | 广西壮族自治区药用植物园 | Cuttage propagation method for anisetree bark |
| CN105875146A (en) * | 2016-04-18 | 2016-08-24 | 杭州富阳飞博科技有限公司 | Vegetative propagation method of torreya jackii |
| CN106386336A (en) * | 2016-08-30 | 2017-02-15 | 四川七彩林业开发有限公司 | Pyracantha fortuneana Harlequin cuttage breeding method |
| CN106416680A (en) * | 2016-09-08 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Melaleuca alternifolia cutting propagation method |
| CN108887162A (en) * | 2018-06-26 | 2018-11-27 | 赤水市信天中药产业开发有限公司 | A kind of HERBA DENDROBII cuttage breeding method |
| CN115053903A (en) * | 2022-06-27 | 2022-09-16 | 菏泽市农业科学院(山东省农业科学院菏泽市分院) | Peony root soaking solution and peony seedling raising method |
| CN115053903B (en) * | 2022-06-27 | 2024-01-26 | 菏泽市农业科学院(山东省农业科学院菏泽市分院) | Paeonia lactiflora root soaking solution and seedling raising method of Paeonia lactiflora |
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Application publication date: 20120919 |