CN106561425A - Cutting propagation method for kmeria septentrionalis - Google Patents
Cutting propagation method for kmeria septentrionalis Download PDFInfo
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- CN106561425A CN106561425A CN201610928396.5A CN201610928396A CN106561425A CN 106561425 A CN106561425 A CN 106561425A CN 201610928396 A CN201610928396 A CN 201610928396A CN 106561425 A CN106561425 A CN 106561425A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a cutting propagation method for kmeria septentrionalis. The method includes the following steps that firstly, half-lignified branches are collected at September to October of every year and are trimmed into cutting slips, after the stem bases of the cutting slips are sterilized, soaking is performed in a rooting inductive agent for 2 h, taking out and drying are performed, and the rooting inductive agent is mainly a mixed aqueous solution of naphthyl acetic acid, indole acetic acid, aspirin and saccharose, wherein the concentration of the naphthyl acetic acid is 100-300 mg/L, the concentration of the indole acetic acid is 500-800 mg/L, the concentration of the aspirin is 50-100 mg/L and the concentration of the saccharose is 15-30 g/L; secondly, the cutting slips are inserted into a seedling bed medium and are immediately irrigated with the rooting inductive agent; and thirdly, a shade frame is erected on a seedling bed, the relative humidity in the seedling bed is controlled at 70-80%, the temperature is 26-28 DEG C, and the light shading rate is 50-60%. The cutting propagation method for the kmeria septentrionalis has the beneficial effects of being high in cutting rooting rate and survival rate, easy and convenient to operate, and the like.
Description
Technical field
The present invention relates to technical field of plant asexual propagation.It is more particularly related to a kind of skewer of Kmeria septentrionalis
Slotting propagation method.
Background technology
Kmeria septentrionalis (Kmeria septentrionalis) is the Evergreen Broad-leaved Tree Species of Magnoliaceae Kmeria, its tree
Body is tall and big, tree-like beautiful, spends pure white fragrance, and material is excellent, is the commerical tree species and flower garden afforestation tree of preciousness.Kmeria septentrionalis is only
The wood of the Libo County Mao Lan Nature Reserves and Guangxi that are distributed in Guizhou discusses Nature Reserve, is distributed in flakes only
See the domestic Guangxi wood in ring river to discuss in Nature Reserve, area about 3hm2, existing 200 plants of the tree that grows into forest, in being listed in
State's one-level national key protected plant.
Kmeria septentrionalis relies primarily on seed and is bred, but under natural conditions, Kmeria septentrionalis percentage of seedgermination is low, and germination is slow
Slowly, its introducing and planting is seriously restricted.The rare report of cottage method to Kmeria septentrionalis, main reason is that Kmeria septentrionalis cuttage is given birth to
Root rate is low, and cuttage is difficult to survive.According to prior art shortcoming, a kind of raising Kmeria septentrionalis cutting plantation is proposed, improve cuttage
The method of survival rate, the effective protection and utilization for the rare or endangered species resource provides technical support.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of cuttage breeding method of Kmeria septentrionalis, it can significantly improve unisexuality
Drymotaenium miyoshianum (Mak.) Mak. cutting plantation and survival rate, it is easy to operate, it is with low cost.
In order to realize these purposes of the invention and further advantage, there is provided a kind of cutting propagation side of Kmeria septentrionalis
Method, comprises the steps：
Step one, cutting collection and process：The annual 9-10 months, the semi-lignified branch of Kmeria septentrionalis plant is gathered, pruned
Into cutting, after cutting base portion is sterilized, 2h is soaked in root induction agent, taken out, then dried to cutting surface in shady place
Without water droplet, wherein,
The root induction agent is mainly the mixed aqueous solution of naphthalene acetic acid, heteroauxing, aspirin and sucrose, wherein,
The concentration of naphthalene acetic acid is 100-300mg/L, and the concentration of heteroauxing is 500-800mg/L, and the concentration of aspirin is 50-
100mg/L, the concentration of sucrose is 15-30g/L；
Step 2, cuttage：Step one gained cutting is inserted in medium of seedling bed, insertion depth is 4-5cm, and seeding row spacing is
10cm × 10cm, pours immediately the root induction agent after cuttage；
Manage after step 3, cuttage：Cool canopy is set up on seedbed, it is 70-80%, temperature to control the relative humidity in seedbed
For 26-28 DEG C, shading rate is 50-60%, and periodically water spray keeps seedbed moistening after cuttage, and sprays 500 times of many bacterium every 7d
Clever solution once, altogether sprays 4 times.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, in step one, has 2- on the semi-lignified branch
3 axillary buds, cutting retains the new blade then of terminal bud and 2, and 2 blades that will be left behind cut 1/2nd, under cutting
End is trimmed to smooth oblique section, and cutting length is 10-15cm.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, in step one, the sterilization of cutting base portion is referred to and will be inserted
Fringe base portion is put in 0.1% potassium permanganate solution and soaks 30min.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, in step 2, medium of seedling bed is by perlite, thin river
Husky and peat soil by volume 1:1:1 is formulated, and the thickness of medium of seedling bed is 15cm, using front, by medium of seedling bed with 800 times
Carbendazim solution carries out disinfection.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, in step 3, manages after cuttage and also include seedling exercising,
Its concrete operations is：After cuttage rooting is stable, every 5d adjustment shading rate 1 time, every time shading rate is reduced into 15-20%, directly
To full exposure.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, also containing nano oxidized in the root induction agent
Zinc and fig extract, wherein, nano oxidized zinc concentration is 10-20mg/L；The concentration of fig extract is 30-
50mg/L；The fig extract is pulled figss, common fig leaf juice and water in mass ratio 2:5:60 mixing, Jing after boiling by intense fire
Slow fire infusion 4h, cooled and filtered is obtained.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, in step one, also includes, is placed in cutting base portion
Before soaking in root induction agent, the root induction agent is placed in into magnetization treatment 10-12h in 250-300 gauss magnetic fields.
Preferably, the cuttage breeding method of described Kmeria septentrionalis, in step one, is also included, in cutting in shady place
Before drying, cutting is placed in into ultraviolet irradiation intensity for 100-120 μ W/cm-2, irradiation 15-20min under wavelength 365nm.
The present invention at least includes following beneficial effect：
Firstth, by processing cutting using root induction agent, bring up to can Kmeria septentrionalis cutting plantation
More than 55%, by selecting suitable seedbed, for root hydrophobic, air-permeability environment is provided, and control the temperature in seedbed, humidity and
Shading rate, for cutting growth favorable environment is provided, so that cuttage survival rate is up to more than 50%；
Secondth, by adding appropriate nano zine oxide and fig extract in root induction agent, will be raw before immersion
Root induction agent is placed in magnetization treatment in magnetic field, and cutting is carried out into ultraviolet irradiation process, can effectively prevent the pathogenic bacteria of cutting
Grow, activate cambial cell, improve endogenous enzyme activity, and then promote cuttage rooting and improve cuttage survival rate；
3rd, the present invention provides a kind of cuttage breeding method of Kmeria septentrionalis, and a large amount of lists can be quickly bred in a short time
Property Drymotaenium miyoshianum (Mak.) Mak. seedling, while having the advantage for keeping maternal merit concurrently, the method is simple to operate, low cost, be easy for workers to operation,
Kmeria septentrionalis implantation in large scale is of very high actual application value, also one kind can be provided for tL septenrwna // s germplasm resource conservation
Effective way, the cultivation for Kmeria septentrionalis is bred and provides sound assurance with the utilization of resources.
The further advantage of the present invention, target and feature embody part by description below, and part will also be by this
The research of invention and practice and be understood by the person skilled in the art.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
It should be noted that experimental technique described in following embodiments, if no special instructions, is conventional method, it is described
Reagent and material, if no special instructions, commercially obtain.
Embodiment 1：
On September 10th, 2015, by the semi-lignified branch for having 2-3 axillary bud on Kmeria septentrionalis plant cutting is cut into.Cutting
Retain the new blade then of terminal bud and 2, and 2 blades that will be left behind cut 1/2nd, lower end is trimmed to smooth oblique
Tangent plane, length is 10-15cm.Cutting base portion is soaked in 0.1% potassium permanganate and is carried out disinfection, disinfecting time is 30min.Will
Cutting after sterilization is processed without any root induction agent, and in being inserted directly into the medium of seedling bed that thickness is 15cm, insertion depth is
4-5cm, seeding row spacing is 10cm × 10cm, is poured to seedbed with clear water immediately after cuttage and is moistened.Medium of seedling bed is Jing carbendazim 800
The homogeneous mixture of the perlite, thin river sand and peat soil of times liquid sterilization treatment, volume ratio is 1:1:1.Shade is set up on seedbed
Canopy, it is 26-28 DEG C that relative humidity is controlled in seedbed for 70-80%, ambient temperature, and shading rate is 50-60%.Periodically spray after cuttage
Water keeps seedbed moistening, and 500 times of carbendazol is sprayed every 7d in one month, prevents pathogenic bacteria from growing.Treat that cuttage rooting is stablized
Afterwards, sunshade net is suitably opened, gradually increases the illuminance of cutting, until full exposure.After cuttage 60 days, statistics rooting percent is
10%, survival rate is 8%.
Embodiment 2：
On September 10th, 2015, by the semi-lignified branch for having 2-3 axillary bud on Kmeria septentrionalis plant cutting is cut into.Cutting
Retain the new blade then of terminal bud and 2, and 2 blades that will be left behind cut 1/2nd, lower end is trimmed to smooth oblique
Tangent plane, length is 10-15cm.Cutting base portion is soaked in 0.1% potassium permanganate and is carried out disinfection, disinfecting time is 30min.Will
Cutting after sterilization is soaked in 2h in root induction agent (ABT root-growing agents, concentration is 100mg/L), dries to cutting in shady place
Surface is without water droplet.By cutting inserting thickness in the medium of seedling bed of 15cm, insertion depth is 4-5cm, seeding row spacing be 10cm ×
10cm, the root induction agent for pouring same concentration after cuttage immediately is moistened to seedbed.Cutting medium goes out for 800 times of liquid of Jing carbendazim
The homogeneous mixture of perlite, thin river sand and peat soil that bacterium is processed, volume ratio is 1:1:1.Cool canopy, seedbed are set up on seedbed
It is interior control relative humidity for 70-80%, ambient temperature be 26-28 DEG C, shading rate is 50-60%.Periodically water spray keeps after cuttage
Seedbed moistens, and 500 times of carbendazol is sprayed every 7d in one month, prevents pathogenic bacteria from growing.After cuttage rooting is stable, suitably
Sunshade net is opened, gradually increases the illuminance of cutting, until full exposure.After cuttage 60 days, statistics rooting percent is 19%, into
Motility rate is 15%.
Embodiment 3：
On September 10th, 2015, by the semi-lignified branch for having 2-3 axillary bud on Kmeria septentrionalis plant cutting is cut into.Cutting
Retain the new blade then of terminal bud and 2, and 2 blades that will be left behind cut 1/2nd, lower end is trimmed to smooth oblique
Tangent plane, length is 10-15cm.Cutting base portion is soaked in 0.1% potassium permanganate and is carried out disinfection, disinfecting time is 30min.Will
Cutting after sterilization is soaked in root induction agent (naphthalene acetic acid 100mg/L, heteroauxing 500mg/L, aspirin 50mg/L, sugarcane
Sugared 15g/L) in 2h, dry to cutting surface without water droplet in shady place.By cutting inserting thickness for 15cm medium of seedling bed in, insert
It is 4-5cm to enter depth, and seeding row spacing is 10cm × 10cm, and the root induction agent for pouring same concentration after cuttage immediately is wet to seedbed
Profit.Cutting medium for 800 times of liquid sterilization treatment of Jing carbendazim perlite, thin river sand and peat soil homogeneous mixture, volume
Than for 1:1:1.Cool canopy is set up on seedbed, it is 26-28 DEG C that relative humidity is controlled in seedbed for 70-80%, ambient temperature, shading
Rate is 50-60%.Periodically water spray keeps seedbed moistening after cuttage, and 500 times of carbendazol is sprayed every 7d in one month, prevents
Pathogenic bacteria grows.After cuttage rooting is stable, sunshade net is suitably opened, gradually increase the illuminance of cutting, until full exposure.Cuttage
After 60 days, statistics rooting percent is 55%, and survival rate is 50%.
Embodiment 4：
On September 25th, 2015, by the semi-lignified branch for having 2-3 axillary bud on Kmeria septentrionalis plant cutting is cut into.Cutting
Retain the new blade then of terminal bud and 2, and 2 blades that will be left behind cut 1/2nd, lower end is trimmed to smooth oblique
Tangent plane, length is 10-15cm.Cutting base portion is soaked in 0.1% potassium permanganate and is carried out disinfection, disinfecting time is 30min.Will
Cutting after sterilization is soaked in root induction agent (naphthalene acetic acid 200mg/L, heteroauxing 600mg/L, aspirin 75mg/L, sugarcane
Sugared 20g/L) in 2h, dry to cutting surface without water droplet in shady place.By cutting inserting thickness for 15cm medium of seedling bed in, insert
It is 4-5cm to enter depth, and seeding row spacing is 10cm × 10cm, and the root induction agent for pouring same concentration after cuttage immediately is wet to seedbed
Profit.Cutting medium for 800 times of liquid sterilization treatment of Jing carbendazim perlite, thin river sand and peat soil homogeneous mixture, volume
Than for 1:1:1.Cool canopy is set up on seedbed, it is 26-28 DEG C that relative humidity is controlled in seedbed for 70-80%, ambient temperature, shading
Rate is 50-60%.Periodically water spray keeps seedbed moistening after cuttage, and 500 times of carbendazol is sprayed every 7d in one month, prevents
Pathogenic bacteria grows.After cuttage rooting is stable, sunshade net is suitably opened, gradually increase the illuminance of cutting, until full exposure.Cuttage
After 60 days, statistics rooting percent is 57%, and survival rate is 52%.
Embodiment 5：
On October 20th, 2015, the semi-lignified branch for having 2-3 axillary bud on Kmeria septentrionalis plant is cut into into cutting.Cutting
Retain the new blade then of terminal bud and 2, and 2 blades that will be left behind cut 1/2nd, lower end is trimmed to smooth oblique
Tangent plane, length is 10-15cm.Cutting base portion is soaked in 0.1% potassium permanganate and is carried out disinfection, disinfecting time is 30min.Will
Cutting after sterilization is soaked in root induction agent (naphthalene acetic acid 300mg/L, heteroauxing 800mg/L, aspirin 100mg/L, sugarcane
Sugared 30g/L) in 2h, dry to cutting surface without water droplet in shady place.By cutting inserting thickness for 15cm medium of seedling bed in, insert
It is 4-5cm to enter depth, and seeding row spacing is 10cm × 10cm, and the root induction agent for pouring same concentration after cuttage immediately is wet to seedbed
Profit.Cutting medium for 800 times of liquid sterilization treatment of Jing carbendazim perlite, thin river sand and peat soil homogeneous mixture, volume
Than for 1:1:1.Cool canopy is set up on seedbed, it is 26-28 DEG C that relative humidity is controlled in seedbed for 70-80%, ambient temperature, shading
Rate is 50-60%.Periodically water spray keeps seedbed moistening after cuttage, and 500 times of carbendazol is sprayed every 7d in one month, prevents
Pathogenic bacteria grows.After cuttage rooting is stable, every 5d adjustment shading rate 1 time, every time shading rate is reduced into 15-20%, until entirely
Illumination.After cuttage 60 days, statistics rooting percent is 58%, and survival rate is 52%.
Embodiment 6：
On the basis of embodiment 5, also containing nano zine oxide and fig extract in the root induction agent, its
In, nano oxidized zinc concentration is 15mg/L；The concentration of fig extract is 40mg/L；The fig extract is without flower
Dried fruitss, common fig leaf juice and water in mass ratio 2:5:60 mixing, the slow fire infusion 4h Jing after boiling by intense fire, cooled and filtered is obtained.Skewer
After inserting 60 days, statistics rooting percent is 66%, and survival rate is 59%.
Embodiment 7：
On the basis of embodiment 6, also include, be placed in root induction agent before immersion in cutting base portion, take root described
Derivant is placed in magnetization treatment 10-12h in 250-300 gauss magnetic fields.After cuttage 60 days, statistics rooting percent is 68%, into
Motility rate is 64%.
Embodiment 8：
On the basis of embodiment 7, also include, in cutting before shady place is dried, cutting is placed in into ultraviolet irradiation intensity
For 100-120 μ W/cm-2, irradiation 15-20min under wavelength 365nm.After cuttage 60 days, statistics rooting percent is 70%, is survived
Rate is 64%.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to completely various suitable the field of the invention, for those skilled in the art, can be easily
Other modification is realized, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details.
Claims (8)
1. a kind of cuttage breeding method of Kmeria septentrionalis, it is characterised in that comprise the steps：
Step one, cutting collection and process：The annual 9-10 months, the semi-lignified branch of Kmeria septentrionalis plant is gathered, be trimmed to slotting
Fringe, after cutting base portion is sterilized, soaks 2h in root induction agent, takes out, and then dries in shady place anhydrous to cutting surface
Drop, wherein,
The root induction agent is mainly the mixed aqueous solution of naphthalene acetic acid, heteroauxing, aspirin and sucrose, wherein, naphthalene second
The concentration of acid is 100-300mg/L, and the concentration of heteroauxing is 500-800mg/L, and the concentration of aspirin is 50-100mg/L,
The concentration of sucrose is 15-30g/L；
Step 2, cuttage：Step one gained cutting is inserted in medium of seedling bed, insertion depth is 4-5cm, seeding row spacing be 10cm ×
10cm, pours immediately the root induction agent after cuttage；
Manage after step 3, cuttage：Cool canopy is set up on seedbed, it is that 70-80%, temperature are to control relative humidity in seedbed
26-28 DEG C, shading rate is 50-60%, and periodically water spray keeps seedbed moistening after cuttage, and sprays 500 times of carbendazim every 7d
Solution once, altogether sprays 4 times.
2. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 1, it is characterised in that in step one, described half is wooden
Change and have on branch 2-3 axillary bud, cutting reservation terminal bud and 2 new blades then, and 2 blades that will be left behind cut two points
One of, cutting lower end is trimmed to smooth oblique section, and cutting length is 10-15cm.
3. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 1, it is characterised in that in step one, by cutting base portion
Sterilization refers to that cutting base portion is put in 0.1% potassium permanganate solution soaks 30min.
4. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 1, it is characterised in that in step 2, medium of seedling bed by
Perlite, thin river sand and peat soil by volume 1:1:1 is formulated, and the thickness of medium of seedling bed is 15cm, using front, by seedbed
Substrate is carried out disinfection with 800 times of carbendazim solutions.
5. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 1, it is characterised in that in step 3, manages after cuttage
Also include seedling exercising, its concrete operations is：After cuttage rooting is stable, every 5d adjustment shading rate 1 time, shading rate is reduced every time
15-20%, until full exposure.
6. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 1, it is characterised in that also contain in the root induction agent
There are nano zine oxide and fig extract, wherein, nano oxidized zinc concentration is 10-20mg/L；Fig extract it is dense
Spend for 30-50mg/L；The fig extract is pulled figss, common fig leaf juice and water in mass ratio 2:5:60 mixing, Jing is military
Fire boils rear slow fire infusion 4h, and cooled and filtered is obtained.
7. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 6, it is characterised in that in step one, also include, is inserting
Fringe base portion is placed in root induction agent before immersion, and the root induction agent is placed in into magnetization treatment 10- in 250-300 gauss magnetic fields
12h。
8. the cuttage breeding method of Kmeria septentrionalis as claimed in claim 7, it is characterised in that in step one, also include, is inserting
Cutting is placed in ultraviolet irradiation intensity for 100-120 μ W/cm by fringe before shady place is dried-2, irradiation 15- under wavelength 365nm
20min。
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