CN110214561B - Rapid division propagation method for saxifraga stolonifera - Google Patents
Rapid division propagation method for saxifraga stolonifera Download PDFInfo
- Publication number
- CN110214561B CN110214561B CN201910659193.4A CN201910659193A CN110214561B CN 110214561 B CN110214561 B CN 110214561B CN 201910659193 A CN201910659193 A CN 201910659193A CN 110214561 B CN110214561 B CN 110214561B
- Authority
- CN
- China
- Prior art keywords
- saxifrage
- rhizome
- culture medium
- propagation method
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 244000288377 Saxifraga stolonifera Species 0.000 title claims abstract description 15
- 235000002953 Saxifraga stolonifera Nutrition 0.000 title claims abstract description 15
- 241001647091 Saxifraga granulata Species 0.000 claims abstract description 60
- 239000001963 growth medium Substances 0.000 claims abstract description 39
- 239000002689 soil Substances 0.000 claims abstract description 32
- 244000153955 Reynoutria sachalinensis Species 0.000 claims abstract description 29
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 claims abstract description 29
- 238000002791 soaking Methods 0.000 claims abstract description 22
- 238000004140 cleaning Methods 0.000 claims abstract description 15
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 14
- 230000002421 anti-septic effect Effects 0.000 claims abstract description 12
- 230000035784 germination Effects 0.000 claims abstract description 10
- 238000005520 cutting process Methods 0.000 claims abstract description 8
- 235000007711 Panicum palmifolium Nutrition 0.000 claims description 18
- 241000569924 Pinanga maculata Species 0.000 claims description 18
- 230000000844 anti-bacterial effect Effects 0.000 claims description 13
- 239000003899 bactericide agent Substances 0.000 claims description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- 239000004576 sand Substances 0.000 claims description 12
- 230000001737 promoting effect Effects 0.000 claims description 8
- 238000010008 shearing Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 6
- 239000005747 Chlorothalonil Substances 0.000 claims description 6
- 229960005070 ascorbic acid Drugs 0.000 claims description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 6
- 239000006013 carbendazim Substances 0.000 claims description 6
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 claims description 6
- 239000003864 humus Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 235000010323 ascorbic acid Nutrition 0.000 claims description 5
- 239000011668 ascorbic acid Substances 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000005260 corrosion Methods 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 230000000644 propagated effect Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 description 3
- 241000415040 Anemone rivularis Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 241000242759 Actiniaria Species 0.000 description 1
- 241001083548 Anemone Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a rapid division propagation method of saxifraga stolonifera, which comprises the following steps of 1) selecting saxifraga stolonifera to be aired; 2) cleaning soil; 3) performing antiseptic treatment; 4) soaking the rhizome of the anti-corrosion processed saxifrage into a plant growth regulator for 2-4 h; 5) cutting, and planting in culture medium; 6) cultivating to obtain the germinated giant knotweed. Firstly, the invention avoids the problem that the saxifrage cannot be propagated in large quantity due to the rare seeds of the saxifrage, simultaneously improves the germination rate of the saxifrage, protects the original seed property of the saxifrage, avoids the variation loss of the saxifrage, obviously improves the propagation speed, increases the seedling amount of the rare Yi medicine of the saxifrage, enlarges the germplasm resource quantity of the saxifrage, and simultaneously can reduce the cost, has simple operation and high economic benefit.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine planting, in particular to a rapid division propagation method of saxifrage.
Background
Aethera diffusa, Anemones rivularis Buch-ham. ex Dc, also called caoyume, Fangqing, Fanglan, is a perennial medicinal plant of Ranunculaceae, and has the effects of clearing away heat and toxic materials, promoting blood circulation, relaxing muscles and tendons, relieving cough, eliminating phlegm, resisting bacteria, resisting cancer and the like. The giant knotweed herb is usually grown on mountainous regions, grass rooms, stream sides or other wetlands or forest edges with the elevation of 1750m to 3300m in Yunnan province. Secondly, the plant is distributed in Sichuan, Guizhou, southwest of Gansu, southeast of Qinghai, southwest of Hubei, western Guangxi, southern Tibet and eastern China, but with the change of environmental conditions, the wild berberian anemone is endangered and died, which causes resource shortage and supply shortage.
The anemone rivularis has low seed setting rate and short seed life under natural conditions, and is easy to lose germination force, and meanwhile, the progeny of the seeds of the anemone rivularis is easy to lose the original seed property and generate variation, so that the pedicel becomes thin, soft, and the flower type becomes small. Therefore, establishing a rapid and efficient saxifrage seedling production technical system has urgent practical significance for the seedling use amount of rare Yi medicine saxifrage and the expansion of the germplasm resource amount of the saxifrage.
Disclosure of Invention
The invention provides a rapid division propagation method of saxifrage.
The scheme of the invention is as follows:
a rapid division propagation method of saxifrage comprises the following steps:
1) selecting and excavating the tiger palm grass, and airing the excavated tiger palm grass in a room until the tiger palm grass is dry;
2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil;
3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 1-15 g/L of a bactericide for 20-40 min;
4) soaking the rhizome of the saxifraga stolonifera subjected to the antiseptic treatment in a plant growth regulator for 2-4 hours, and promoting germination of the hidden bud on the rhizome of the saxifraga stolonifera after soaking;
5) cutting the rhizome of each piece of the giant knotweed treated in the step 4) into 4-5 sections, and planting the cut rhizome of the giant knotweed in a planting culture medium respectively;
6) the method comprises the steps of paving a film on a planting culture medium planted with the tuber of the saxifrage, storing the film in a seedling shed, controlling the temperature to be 25-30 ℃ in the daytime, controlling the air humidity to be 90-100%, controlling the water content of the planting culture medium to be 60-80%, and cultivating for 7-15 days to obtain the germinated and grown saxifrage.
As a preferred technical scheme, the bactericide comprises one or more mixed solutions of chlorothalonil and carbendazim.
According to a preferable technical scheme, the plant growth regulator comprises 0.04-40 ml/L of mixed culture medium and 0.1-5.0 g/L of ascorbic acid.
As a preferred technical scheme, the mixed culture medium comprises 6-BA, NAA, IBA, IAA, Zt and Kt.
As a preferred technical scheme, the planting culture substrate comprises sand and soil, and the feeding ratio of the sand to the soil is 1: 1.
As a preferred technical scheme, the soil is humus soil.
As a preferable technical scheme, the step 6) further comprises the step of illuminating for 8-10 hours/day.
Preferably, the step 6) further comprises irradiating scattered light for irradiation.
As a preferable technical scheme, when airing in the step 1), the giant knotweed should be spread out and aired uniformly, and the giant knotweed should be turned over once at intervals of 10-20 min.
Due to the adoption of the technical scheme, the rapid division breeding method of the saxifraga stolonifera comprises the following steps of 1) selecting the saxifraga stolonifera for excavation, placing the excavated saxifraga stolonifera in a room for airing, and airing to a five-dry state; 2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil; 3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 1-15 g/L of a bactericide for 20-40 min; 4) soaking the rhizome of the saxifraga stolonifera subjected to the antiseptic treatment in a plant growth regulator for 2-4 hours, and promoting germination of the hidden bud on the rhizome of the saxifraga stolonifera after soaking; 5) cutting the rhizome of each piece of the giant knotweed treated in the step 4) into 4-5 sections, and planting the cut rhizome of the giant knotweed in a planting culture medium respectively; 6) the method comprises the steps of paving a film on a planting culture medium planted with the tuber of the saxifrage, storing the film in a seedling shed, controlling the temperature to be 25-30 ℃ in the daytime, controlling the air humidity to be 90-100%, controlling the water content of the planting culture medium to be 60-80%, and cultivating for 7-15 days to obtain the germinated and grown saxifrage.
The invention has the advantages that:
firstly, the invention avoids the problem that the saxifrage cannot be propagated in large quantity due to the rare seeds of the saxifrage, simultaneously improves the germination rate of the saxifrage, protects the original seed property of the saxifrage, avoids the variation loss of the saxifrage, obviously improves the propagation speed, increases the seedling using amount of the rare Yi medicine of the saxifrage, enlarges the germplasm resource quantity of the saxifrage, and simultaneously can reduce the cost, has simple operation and high economic benefit.
Detailed Description
In order to make up for the above disadvantages, the invention provides a rapid division breeding method of saxifrage to solve the problems in the background art.
A rapid division propagation method of saxifrage comprises the following steps:
1) selecting and excavating the tiger palm grass, and airing the excavated tiger palm grass in a room until the tiger palm grass is dry;
2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil;
3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 1-15 g/L of a bactericide for 20-40 min;
4) soaking the rhizome of the saxifraga stolonifera subjected to the antiseptic treatment in a plant growth regulator for 2-4 hours, and promoting germination of the hidden bud on the rhizome of the saxifraga stolonifera after soaking;
5) cutting the rhizome of each piece of the giant knotweed treated in the step 4) into 4-5 sections, and planting the cut rhizome of the giant knotweed in a planting culture medium respectively;
6) the method comprises the steps of paving a film on a planting culture medium planted with the tuber of the saxifrage, storing the film in a seedling shed, controlling the temperature to be 25-30 ℃ in the daytime, controlling the air humidity to be 90-100%, controlling the water content of the planting culture medium to be 60-80%, and cultivating for 7-15 days to obtain the germinated and grown saxifrage.
The bactericide comprises one or more mixed solutions of chlorothalonil and carbendazim.
The plant growth regulator comprises 0.04-40 ml/L of mixed culture medium and 0.1-5.0 g/L of ascorbic acid.
The mixed culture medium comprises 6-BA, NAA, IBA, IAA, Zt and Kt.
The planting culture medium comprises sand and soil, and the feeding ratio of the sand to the soil is 1: 1.
The soil is humus soil.
The step 6) also comprises that the illumination time is 8-10 hours/day.
The step 6) also comprises the irradiation treatment of scattered light.
In the step 1), when airing, the giant knotweed should be spread out and aired uniformly, and the giant knotweed should be turned over once at intervals of 10-20 min.
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1:
a rapid division propagation method of saxifrage comprises the following steps:
1) selecting and excavating the tiger palm grass, and airing the excavated tiger palm grass in a room until the tiger palm grass is dry;
2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil;
3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 1g/L bactericide for 20 min;
4) soaking the rhizome of the anti-corrosion processed saxifrage in a plant growth regulator for 2h, and promoting the germination of the hidden bud on the rhizome of the saxifrage after the soaking is finished;
5) cutting the rhizome of each saxatilis emelianov processed in the step 4) into 4 sections, and planting the cut rhizome of each saxatilis emelianov in a planting culture medium respectively;
6) the method comprises the steps of laying a film on a planting culture medium planted with the rhizomes of the saxifrage, storing the film in a seedling shed, controlling the temperature at 25 ℃ in the daytime and the air humidity at 90%, controlling the water content of the planting culture medium at 60%, and culturing for 7 days to obtain the germinated saxifrage.
The bactericide comprises one or more mixed solutions of chlorothalonil and carbendazim.
The plant growth regulator comprises 0.04ml/L mixed culture medium and 0.1g/L ascorbic acid.
The mixed culture medium comprises 6-BA, NAA, IBA, IAA, Zt and Kt.
The planting culture medium comprises sand and soil, and the feeding ratio of the sand to the soil is 1: 1.
The soil is humus soil.
The step 6) also comprises that the illumination time is 8 hours/day.
The step 6) also comprises the irradiation treatment of scattered light.
In the step 1), when airing, the giant knotweed should be spread out and aired uniformly, and the giant knotweed should be turned over once at intervals of 10 min.
Example 2:
a rapid division propagation method of saxifrage comprises the following steps:
1) selecting and excavating the tiger palm grass, and airing the excavated tiger palm grass in a room until the tiger palm grass is dry;
2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil;
3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 15g/L bactericide for 40 min;
4) soaking the rhizome of the anti-corrosion processed saxifrage in the plant growth regulator for 4h, and promoting the germination of the hidden bud on the rhizome of the saxifrage after the soaking is finished;
5) cutting the rhizome of each saxatilis emelianov processed in the step 4) into 5 sections, and planting the cut rhizome of each saxatilis emelianov in a planting culture medium respectively;
6) spreading a film on a planting culture medium planted with the rhizome of the saxifrage, storing in a seedling shed, controlling the temperature at 30 ℃ in the daytime, controlling the air humidity at 100%, controlling the water content of the planting culture medium at 80%, and culturing for 15 days to obtain the germinated saxifrage.
The bactericide comprises one or more mixed solutions of chlorothalonil and carbendazim.
The plant growth regulator comprises 40ml/L of mixed culture medium and 5.0g/L of ascorbic acid.
The mixed culture medium comprises 6-BA, NAA, IBA, IAA, Zt and Kt.
The planting culture medium comprises sand and soil, and the feeding ratio of the sand to the soil is 1: 1.
The soil is humus soil.
The step 6) also comprises that the illumination time is 10 hours/day.
The step 6) also comprises the irradiation treatment of scattered light.
In the step 1), when airing, the giant knotweed should be spread out and aired uniformly, and the giant knotweed should be turned over once at intervals of 20 min.
Example 3:
a rapid division propagation method of saxifrage comprises the following steps:
1) selecting and excavating the tiger palm grass, and airing the excavated tiger palm grass in a room until the tiger palm grass is dry;
2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil;
3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 10g/L bactericide for 30 min;
4) soaking the rhizome of the anti-corrosion processed saxifrage in the plant growth regulator for 3h, and promoting the germination of the hidden bud on the rhizome of the saxifrage after the soaking is finished;
5) cutting the rhizome of each saxatilis emelianov processed in the step 4) into 4 sections, and planting the cut rhizome of each saxatilis emelianov in a planting culture medium respectively;
6) spreading a film on a planting culture medium planted with the rhizome of the saxifrage, storing in a seedling shed, controlling the temperature at 28 ℃ in the daytime, controlling the air humidity at 100%, controlling the water content of the planting culture medium at 70%, and culturing for 10 days to obtain the germinated and grown saxifrage.
The bactericide comprises one or more mixed solutions of chlorothalonil and carbendazim.
The plant growth regulator comprises 20ml/L of mixed culture medium and 2.0g/L of ascorbic acid.
The mixed culture medium comprises 6-BA, NAA, IBA, IAA, Zt and Kt.
The planting culture medium comprises sand and soil, and the feeding ratio of the sand to the soil is 1: 1.
The soil is humus soil.
The step 6) also comprises that the illumination time is 9 hours/day.
The step 6) also comprises the irradiation treatment of scattered light.
In the step 1), when airing, the giant knotweed should be spread out and aired uniformly, and the giant knotweed should be turned over once at intervals of 15 min.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. A rapid division propagation method of saxifrage is characterized by comprising the following steps:
1) selecting and excavating the tiger palm grass, and airing the excavated tiger palm grass in a room until the tiger palm grass is dry;
2) cleaning and shearing dead leaves on the rhizome of the five-dry-state giant knotweed, and then cleaning soil;
3) performing antiseptic treatment, namely soaking the cleaned rhizome of the saxifrage into 1-15 g/L of a bactericide for 20-40 min;
4) soaking the rhizome of the saxifraga stolonifera subjected to the antiseptic treatment in a plant growth regulator for 2-4 hours, and promoting germination of the hidden bud on the rhizome of the saxifraga stolonifera after soaking;
5) cutting the rhizome of each tuber of giant knotweed treated in the step 4) into 4-5 sections, and planting the cut rhizome of the giant knotweed in a planting culture medium respectively, wherein the planting culture medium comprises sand and soil, the feeding ratio of the sand to the soil is 1:1, and the soil is humus soil;
6) the method comprises the steps of paving a film on a planting culture medium planted with the tuber of the saxifrage, storing the film in a seedling shed, controlling the temperature to be 25-30 ℃ in the daytime, controlling the air humidity to be 90-100%, controlling the water content of the planting culture medium to be 60-80%, and cultivating for 7-15 days to obtain the germinated and grown saxifrage.
2. The rapid division propagation method of saxifrage as claimed in claim 1, characterized in that: the bactericide comprises one or more mixed solutions of chlorothalonil and carbendazim.
3. The rapid division propagation method of saxifrage as claimed in claim 1, characterized in that: the plant growth regulator comprises 0.04-40 ml/L of mixed culture medium and 0.1-5.0 g/L of ascorbic acid.
4. The rapid division propagation method of saxifrage as claimed in claim 3, characterized in that: the mixed culture medium comprises 6-BA, NAA, IBA, IAA, Zt and Kt.
5. The rapid division propagation method of saxifrage as claimed in claim 1, characterized in that: the step 6) also comprises that the illumination time is 8-10 hours/day.
6. The rapid division propagation method of saxifrage as claimed in claim 1, characterized in that: the step 6) also comprises the irradiation treatment of scattered light.
7. The rapid division propagation method of saxifrage as claimed in claim 1, characterized in that: in the step 1), when airing, the giant knotweed should be spread out and aired uniformly, and the giant knotweed should be turned over once at intervals of 10-20 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910659193.4A CN110214561B (en) | 2019-07-22 | 2019-07-22 | Rapid division propagation method for saxifraga stolonifera |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910659193.4A CN110214561B (en) | 2019-07-22 | 2019-07-22 | Rapid division propagation method for saxifraga stolonifera |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110214561A CN110214561A (en) | 2019-09-10 |
| CN110214561B true CN110214561B (en) | 2021-06-15 |
Family
ID=67813674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910659193.4A Expired - Fee Related CN110214561B (en) | 2019-07-22 | 2019-07-22 | Rapid division propagation method for saxifraga stolonifera |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110214561B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226518B (en) * | 2019-07-16 | 2022-05-06 | 楚雄师范学院 | Tissue culture and rapid propagation method of saxifrage |
| CN113767764A (en) * | 2021-09-18 | 2021-12-10 | 深圳文科园林股份有限公司 | Grateloupia filicina seedling raising method and equipment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101755593A (en) * | 2009-11-12 | 2010-06-30 | 天津滨海国际花卉科技园区股份有限公司 | Method for dividing and breeding Calathea veitchiana |
| CN101940122A (en) * | 2010-09-08 | 2011-01-12 | 天津滨海国际花卉科技园区股份有限公司 | Division propagation method of anthurium |
-
2019
- 2019-07-22 CN CN201910659193.4A patent/CN110214561B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101755593A (en) * | 2009-11-12 | 2010-06-30 | 天津滨海国际花卉科技园区股份有限公司 | Method for dividing and breeding Calathea veitchiana |
| CN101940122A (en) * | 2010-09-08 | 2011-01-12 | 天津滨海国际花卉科技园区股份有限公司 | Division propagation method of anthurium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110214561A (en) | 2019-09-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102599026B (en) | Tilia miqueliana seedling raising method | |
| CN101036446A (en) | Quick propagating method of megranate | |
| WO2020107888A1 (en) | Ex vitro rooting method for acer pentaphyllum tissue culture | |
| CN106561425A (en) | Cutting propagation method for kmeria septentrionalis | |
| CN104798670A (en) | Non-woven fabric container cutting seedling raising method of Fraxinus velutina twigs | |
| CN108307911B (en) | Root cutting seedling raising method for fish wood | |
| CN110214561B (en) | Rapid division propagation method for saxifraga stolonifera | |
| CN106577216B (en) | Method for promoting germination of lateral buds of succulent plants in Crassulaceae | |
| CN104904555B (en) | A kind of making method for its potted landscape of red maple of growing directly from seeds | |
| CN107889576B (en) | Method for quickly and efficiently breeding pittosporum tobira seedlings | |
| CN109220261A (en) | A kind of dove tree seedling breeding method | |
| Gautam et al. | Studies on Clonal Multiplication of Guava ('Psidium guajava'L.) through Cutting under Controlled Conditions | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN106900307A (en) | A kind of division propagation method of jade hairpin | |
| CN107409896B (en) | Sowing and seedling raising method for golden panda trees | |
| CN108633596A (en) | A kind of implantation methods of deep gloomy Chinese scholartree fast-growth | |
| CN104737747A (en) | Propagation and culture method for red maple seedlings | |
| CN105900805B (en) | Soilless culture technology for peony | |
| CN104686127B (en) | Introduce sleep cloth bag rhizome cutting propagation method in a kind of Africa | |
| CN1303870C (en) | Culture method of Sinningia speciosa | |
| CN108633379B (en) | Method for breaking dormancy of cercis gigantea seeds | |
| CN112314104A (en) | Sabina przewalskii seed germination accelerating and seedling cultivation method | |
| CN107360756B (en) | Method for promoting rapid and multiple germination of golden plum seeds into seedlings | |
| CN104521521A (en) | Tillering reproduction method for sago cycas | |
| CN109089575A (en) | The cottage method of clematis Poland spirit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210615 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |