CN110214561A - A kind of quick division propagation method of brook anemone - Google Patents
A kind of quick division propagation method of brook anemone Download PDFInfo
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- CN110214561A CN110214561A CN201910659193.4A CN201910659193A CN110214561A CN 110214561 A CN110214561 A CN 110214561A CN 201910659193 A CN201910659193 A CN 201910659193A CN 110214561 A CN110214561 A CN 110214561A
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- brook anemone
- anemone
- brook
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of quick division propagation method of brook anemone, 1) brook anemone is chosen into sunning；2) soil is cleaned；3) preservative treatment；4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 2~4h in plant growth regulator；5) it cuts, plants in culture substrate；6) it cultivates, obtains sprouting the brook anemone grown up to.First; the invention avoids because of brook anemone seed rareness, to cause the problem of brook anemone cannot get mass propagation, while the germination rate of brook anemone is improved; protect the original kind of property of brook anemone; brook anemone is avoided to morph loss, reproduction speed is significantly improved, and rare Yi nationality's medicine brook anemone is made to measure increasing with seedling; expand the germ plasm resource quantity of brook anemone; it can reduce cost simultaneously, easy to operate, high financial profit.
Description
Technical field
The present invention relates to Chinese medicine plant technical field more particularly to a kind of quick division propagation methods of brook anemone.
Background technique
Brook anemone Anemones rivularis Buch.-ham.ex Dc is called anemone rivularis, sees that wind is green, sees wind indigo plant, is hair
Gelsemium section perennial herb medicinal plant, has clearing heat and detoxicating, activates blood and relax tendons, antibechic, eliminating the phlegm, antibacterial, anticancer and other effects.Brook anemone
It is often born between the mountainous region of Yunnan height above sea level 1750m~3300m, grass, by small stream or waits wetlands or border.Secondly in Sichuan, Guizhou, Gansu
The ground such as the west and south, southeast Qinghai, the Hubei west and south, West Guangxi, southern Tibet and east are also distributed, but with environment item
Part changes, and current wild brook anemone is endangered, causes shortage of resources, supply falls short of demand.
Setting percentage is very low under field conditions (factors) and seed longeivity is short, easily loses germination for brook anemone, while its seed progeny
Breeding is easy to lose original kind of property, morphs, causes bennet to attenuate, softens, flower pattern becomes smaller.Therefore, it establishes rapidly and efficiently
Brook anemone seeling industry technical system to rare Yi nationality's medicine brook anemone seedling amount and expand brook anemone germ plasm resource quantity all have
There is urgent realistic meaning.
Summary of the invention
Of the invention provides a kind of quick division propagation method of brook anemone.
The scheme of the invention is:
A kind of quick division propagation method of brook anemone, including the following steps:
1) it chooses brook anemone to be excavated, the brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；
2) the dead leaf cleaning on the rhizomes of fifty percent dry state brook anemone is wiped out, then cleans soil；
3) rhizomes of the brook anemone after cleaning is put into 20~40min of immersion in 1~15g/L fungicide by preservative treatment；
4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 2~4h in plant growth regulator, impregnates and completes
The kryptoblast on the rhizomes of brook anemone is promoted to sprout afterwards；
5) rhizomes of the every plant of brook anemone handled by step 4) is cut into 4~5 sections, by the brook anemone after cutting
Rhizomes is transplanted respectively in plantation culture substrate；
6) plantation is had into bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, daytime temperature
Degree control is at 25~30 DEG C, and 90%~100%, the water content for planting culture substrate is 60%~80% for air humidity control,
It was cultivated by 7~15 days, obtains sprouting the brook anemone grown up to.
The fungicide includes that Bravo, carbendazim are one such or a variety of mixing are molten as a preferred technical solution,
Liquid.
As a preferred technical solution, the plant growth regulator include 0.04~40ml/L mixed culture medium with
The ascorbic acid of 0.1~5.0g/L.
The mixed culture medium includes 6-BA, NAA, IBA, IAA, Zt and Kt as a preferred technical solution,.
The plantation culture substrate includes husky and soil as a preferred technical solution, and the husky feed ratio with soil is 1:
1。
The soil is humus soil as a preferred technical solution,.
Further include in the step 6) as a preferred technical solution, light application time be 8-10 hours/day.
It as a preferred technical solution, further include illumination scattering light lighting process in the step 6).
Brook anemone is spread out uniform sunning when the step 1) dries as a preferred technical solution, interval 10~
20min is stirred once.
By adopting the above-described technical solution, a kind of quick division propagation method of brook anemone, 1) brook anemone is chosen to be excavated,
The brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；2) by the rhizomes of fifty percent dry state brook anemone
On dead leaf cleaning wipe out, then clean soil；3) rhizomes of the brook anemone after cleaning is put into 1~15g/L by preservative treatment
20~40min is impregnated in fungicide；4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 2 in plant growth regulator
~4h promotes the kryptoblast on the rhizomes of brook anemone to sprout after the completion of impregnating；5) the every plant of brook anemone that will be handled by step 4)
Rhizomes cut into 4~5 sections, by the rhizomes of the brook anemone after cutting transplant respectively in plantation culture substrate in；6) it will plant
Kind has bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, and day temperature is controlled 25~30
DEG C, 90%~100%, the water content for planting culture substrate is 60%~80% for air humidity control, is trained by 7~15 days
It educates, obtains sprouting the brook anemone grown up to.
Advantages of the present invention:
Firstly, the invention avoids because of brook anemone seed rareness, so that causing brook anemone cannot get prolific ask
Topic, while improving the germination rate of brook anemone, protects the original kind of property of brook anemone, avoids brook anemone from morphing loss, breeds
Speed is significantly improved, and rare Yi nationality's medicine brook anemone is made to measure increase with seedling, expands the germ plasm resource quantity of brook anemone,
It can reduce cost simultaneously, easy to operate, high financial profit.
Specific embodiment
In order to make up the above deficiency, the present invention provides a kind of quick division propagation methods of brook anemone to solve above-mentioned background skill
The problems in art.
A kind of quick division propagation method of brook anemone, including the following steps:
1) it chooses brook anemone to be excavated, the brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；
2) the dead leaf cleaning on the rhizomes of fifty percent dry state brook anemone is wiped out, then cleans soil；
3) rhizomes of the brook anemone after cleaning is put into 20~40min of immersion in 1~15g/L fungicide by preservative treatment；
4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 2~4h in plant growth regulator, impregnates and completes
The kryptoblast on the rhizomes of brook anemone is promoted to sprout afterwards；
5) rhizomes of the every plant of brook anemone handled by step 4) is cut into 4~5 sections, by the brook anemone after cutting
Rhizomes is transplanted respectively in plantation culture substrate；
6) plantation is had into bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, daytime temperature
Degree control is at 25~30 DEG C, and 90%~100%, the water content for planting culture substrate is 60%~80% for air humidity control,
It was cultivated by 7~15 days, obtains sprouting the brook anemone grown up to.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The plant growth regulator includes the mixed culture medium of 0.04~40ml/L and the Vitamin C of 0.1~5.0g/L
Acid.
The mixed culture medium includes 6-BA, NAA, IBA, IAA, Zt and Kt.
The plantation culture substrate includes husky and soil, and the husky feed ratio with soil is 1:1.
The soil is humus soil.
Further include in the step 6) light application time be 8-10 hours/day.
It further include illumination scattering light lighting process in the step 6).
Brook anemone is spread out uniform sunning when the step 1) dries, and 10~20min of interval is stirred once.
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1:
A kind of quick division propagation method of brook anemone, including the following steps:
1) it chooses brook anemone to be excavated, the brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；
2) the dead leaf cleaning on the rhizomes of fifty percent dry state brook anemone is wiped out, then cleans soil；
3) rhizomes of the brook anemone after cleaning is put into 1g/L fungicide and impregnates 20min by preservative treatment；
4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 2h in plant growth regulator, promoted after the completion of impregnating
Sprout the kryptoblast on the rhizomes of brook anemone；
5) rhizomes of the every plant of brook anemone handled by step 4) is cut into 4 sections, by the root of the brook anemone after cutting
Shape stem is transplanted respectively in plantation culture substrate；
6) plantation is had into bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, daytime temperature
Degree control is at 25 DEG C, and 90%%, the water content for planting culture substrate is 60%% for air humidity control, cultivated, obtains by 7 days
The brook anemone grown up to sprouting.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The plant growth regulator includes the mixed culture medium of 0.04ml/L and the ascorbic acid of 0.1g/L.
The mixed culture medium includes 6-BA, NAA, IBA, IAA, Zt and Kt.
The plantation culture substrate includes husky and soil, and the husky feed ratio with soil is 1:1.
The soil is humus soil.
Further include in the step 6) light application time be 8 hours/day.
It further include illumination scattering light lighting process in the step 6).
Brook anemone is spread out uniform sunning when the step 1) dries, and interval 10min is stirred once.
Embodiment 2:
A kind of quick division propagation method of brook anemone, including the following steps:
1) it chooses brook anemone to be excavated, the brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；
2) the dead leaf cleaning on the rhizomes of fifty percent dry state brook anemone is wiped out, then cleans soil；
3) rhizomes of the brook anemone after cleaning is put into 15g/L fungicide and impregnates 40min by preservative treatment；
4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 4h in plant growth regulator, promoted after the completion of impregnating
Sprout the kryptoblast on the rhizomes of brook anemone；
5) rhizomes of the every plant of brook anemone handled by step 4) is cut into 5 sections, by the root of the brook anemone after cutting
Shape stem is transplanted respectively in plantation culture substrate；
6) plantation is had into bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, daytime temperature
Degree control is at 30 DEG C, and 100%, the water content for planting culture substrate is 80% for air humidity control, cultivated, obtains by 15 days
Sprout the brook anemone grown up to.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The plant growth regulator includes the mixed culture medium of 40ml/L and the ascorbic acid of 5.0g/L.
The mixed culture medium includes 6-BA, NAA, IBA, IAA, Zt and Kt.
The plantation culture substrate includes husky and soil, and the husky feed ratio with soil is 1:1.
The soil is humus soil.
Further include in the step 6) light application time be 10 hours/day.
It further include illumination scattering light lighting process in the step 6).
Brook anemone is spread out uniform sunning when the step 1) dries, and interval 20min is stirred once.
Embodiment 3:
A kind of quick division propagation method of brook anemone, including the following steps:
1) it chooses brook anemone to be excavated, the brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；
2) the dead leaf cleaning on the rhizomes of fifty percent dry state brook anemone is wiped out, then cleans soil；
3) rhizomes of the brook anemone after cleaning is put into 10g/L fungicide and impregnates 30min by preservative treatment；
4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 3h in plant growth regulator, promoted after the completion of impregnating
Sprout the kryptoblast on the rhizomes of brook anemone；
5) rhizomes of the every plant of brook anemone handled by step 4) is cut into 4 sections, by the root of the brook anemone after cutting
Shape stem is transplanted respectively in plantation culture substrate；
6) plantation is had into bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, daytime temperature
Degree control is at 28 DEG C, and 100%, the water content for planting culture substrate is 70% for air humidity control, cultivated, obtains by 10 days
Sprout the brook anemone grown up to.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The plant growth regulator includes the mixed culture medium of 20ml/L and the ascorbic acid of 2.0g/L.
The mixed culture medium includes 6-BA, NAA, IBA, IAA, Zt and Kt.
The plantation culture substrate includes husky and soil, and the husky feed ratio with soil is 1:1.
The soil is humus soil.
Further include in the step 6) light application time be 9 hours/day.
It further include illumination scattering light lighting process in the step 6).
Brook anemone is spread out uniform sunning when the step 1) dries, and interval 15min is stirred once.
The above shows and describes the basic principle, main features and advantages of the invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent circle.
Claims (9)
1. a kind of quick division propagation method of brook anemone, characterized in that it comprises the following steps:
1) it chooses brook anemone to be excavated, the brook anemone excavated is put into interior and is dried, sunning to fifty percent dry state；
2) the dead leaf cleaning on the rhizomes of fifty percent dry state brook anemone is wiped out, then cleans soil；
3) rhizomes of the brook anemone after cleaning is put into 20~40min of immersion in 1~15g/L fungicide by preservative treatment；
4) rhizomes for the brook anemone that preservative treatment finishes is immersed in 2~4h in plant growth regulator, promoted after the completion of impregnating
Sprout the kryptoblast on the rhizomes of brook anemone；
5) rhizomes of the every plant of brook anemone handled by step 4) is cut into 4~5 sections, by the root shape of the brook anemone after cutting
Stem is transplanted respectively in plantation culture substrate；
6) plantation is had to bedding film on the plantation culture substrate of brook anemone rhizomes, is stored in seedling growth greenhouse, day temperature control
System is at 25~30 DEG C, and air humidity control is 90%~100%, and the water content for planting culture substrate is 60%~80%, by 7
It cultivates within~15 days, obtains sprouting the brook anemone grown up to.
2. a kind of quick division propagation method of brook anemone as described in claim 1, it is characterised in that: the fungicide includes hundred bacterium
Clearly, carbendazim is one such or a variety of mixed solutions.
3. a kind of quick division propagation method of brook anemone as described in claim 1, it is characterised in that: the plant growth regulator
The ascorbic acid of mixed culture medium and 0.1~5.0g/L including 0.04~40ml/L.
4. a kind of quick division propagation method of brook anemone as claimed in claim 3, it is characterised in that: the mixed culture medium includes
6-BA, NAA, IBA, IAA, Zt and Kt.
5. a kind of quick division propagation method of brook anemone as described in claim 1, it is characterised in that: the plantation culture substrate packet
Husky and soil is included, the husky feed ratio with soil is 1:1.
6. a kind of quick division propagation method of brook anemone as claimed in claim 5, it is characterised in that: the soil is humus soil
Earth.
7. a kind of quick division propagation method of brook anemone as described in claim 1, it is characterised in that: further include in the step 6)
Light application time is 8-10 hours/day.
8. a kind of quick division propagation method of brook anemone as described in claim 1, it is characterised in that: further include in the step 6)
Illumination scattering light lighting process.
9. a kind of quick division propagation method of brook anemone as described in claim 1, it is characterised in that: the step 1) is wanted when drying
Spread brook anemone out uniform sunning, 10~20min of interval is stirred once.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910659193.4A CN110214561B (en) | 2019-07-22 | 2019-07-22 | Rapid division propagation method for saxifraga stolonifera |
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| CN201910659193.4A CN110214561B (en) | 2019-07-22 | 2019-07-22 | Rapid division propagation method for saxifraga stolonifera |
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| CN110214561B CN110214561B (en) | 2021-06-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110226518A (en) * | 2019-07-16 | 2019-09-13 | 楚雄师范学院 | A kind of tissue culture and rapid propagation method of brook anemone |
| CN113767764A (en) * | 2021-09-18 | 2021-12-10 | 深圳文科园林股份有限公司 | Grateloupia filicina seedling raising method and equipment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226518A (en) * | 2019-07-16 | 2019-09-13 | 楚雄师范学院 | A kind of tissue culture and rapid propagation method of brook anemone |
| CN110226518B (en) * | 2019-07-16 | 2022-05-06 | 楚雄师范学院 | Tissue culture and rapid propagation method of saxifrage |
| CN113767764A (en) * | 2021-09-18 | 2021-12-10 | 深圳文科园林股份有限公司 | Grateloupia filicina seedling raising method and equipment |
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