CN113767764A - Grateloupia filicina seedling raising method and equipment - Google Patents

Grateloupia filicina seedling raising method and equipment Download PDF

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Publication number
CN113767764A
CN113767764A CN202111097302.1A CN202111097302A CN113767764A CN 113767764 A CN113767764 A CN 113767764A CN 202111097302 A CN202111097302 A CN 202111097302A CN 113767764 A CN113767764 A CN 113767764A
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grass
culture
ciliate desert
seedling
plant
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蔡金术
路洋
郑卫国
曹华英
宫彦章
许诺
朱江丽
张正蕊
李朗
程艳
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Shenzhen Wenke Landscape Co Ltd
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Shenzhen Wenke Landscape Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
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    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14Greenhouses
    • A01G9/16Dismountable or portable greenhouses ; Greenhouses with sliding roofs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • A01G9/247Watering arrangements
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A01N43/42Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647Triazoles; Hydrogenated triazoles
    • A01N43/6531,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
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    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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Abstract

The invention discloses a ciliate desert-grass seedling method and equipment, cutting the rhizome of a ciliate desert-grass mother plant into sub-plants; sequentially and respectively soaking the strains in a carbendazim solution, a copper quinoline copper tebuconazole solution and an amino acid water-soluble fertilizer; placing the daughter plant into a plug tray, and covering the daughter plant in the plug tray with a culture medium; and performing moisture preservation culture, temperature control culture and light supplement culture on the strains in the plug tray. The method is beneficial to greatly improving the germination rate and the seedling rate of the sub-plants, shortening the seedling time, breaking through the seasonal limitation of the plant-divided seedling of the ciliate desert-grass and realizing annual production.

Description

Grateloupia filicina seedling raising method and equipment
Technical Field
The invention relates to the technical field of pteridophyte seedling culture, in particular to a pteris vittata seedling culture method and device.
Background
Ciliate desert-grass is a kind of terrestrial fern, and is widely distributed in warm and humid mountain forest in tropical and subtropical areas in the south of Qinling mountain in China. As an arsenic super-enrichment plant, the arsenic super-enrichment plant has great potential in the technology of restoring soil polluted by compound heavy metal, and has been successfully applied to the field restoration of the soil of the polluted farmland in the southern mining area of China. Meanwhile, the ciliate desert-grass can be used as a pot culture for appreciation, the feathery compound leaves of the ciliate desert-grass are extremely beautiful and evergreen in four seasons, are relatively shady, and are one of the popular indoor leaf-watching plants.
Since ciliate desert-grass is not flowering and fruitless, it can not be propagated by seeds. In the prior art, the propagation of ciliate desert-grass generally adopts three methods of mature spore propagation, plant division propagation and tissue culture propagation. Tiny spores need to be treated in advance during spore propagation, the seeding quantity is not easy to control, seedling culture failure is easy to cause due to mildew infection through high-density seeding of spore powder, and the spore germination rate and the germination time are extremely easy to be influenced by environmental factors. The operation of tissue culture and spore propagation is more complicated, the requirements on the professional and technical properties of operators are higher, and the plant division propagation is widely applied to the propagation of the ciliate desert-grass due to the characteristics of fast seedling formation and low operation technical requirements. But the time suitable for the propagation of the plant is limited, mainly focusing on 5-6 months; the propagation coefficient of the plant division propagation is lower, generally, only 2-3 plants can be propagated by one stock plant, and the propagation material waste is serious.
Disclosure of Invention
The invention mainly aims to provide a ciliate desert-grass seedling raising method and equipment, and aims to solve the technical problems of limitation of ciliate desert-grass propagation expanding operation time and low propagation expanding coefficient.
In order to achieve the purpose, the invention provides a ciliate desert-grass seedling culture method, which comprises the following steps:
cutting the rhizome of the ciliate desert-grass mother plant into sub-plants;
sequentially and respectively soaking the strains in a carbendazim solution, a copper quinoline copper tebuconazole solution and an amino acid water-soluble fertilizer;
placing the daughter plant into a plug tray, and covering the daughter plant in the plug tray with a culture medium;
and performing moisture preservation culture, temperature control culture and light supplement culture on the strains in the plug tray.
Preferably, the daughter strain is 1-5cm in length and/or comprises 1-3 germinating nodes.
Preferably, the concentration of the carbendazim solution is 1000-2000 times of the solution, and the soaking time is 5-30 minutes; the concentration of the quinoline copper tebuconazole copper preparation solution is 1000-2000 times of that of the solution, and the soaking time is 5-10 minutes; the concentration of the amino acid water-soluble fertilizer is 50-100 times of the concentration of the amino acid water-soluble fertilizer, and the soaking time is 5-10 minutes.
Preferably, the culture medium is a mixture of peat soil and hydrated lime with the mass ratio of 100 kg: 1-10 kg.
Preferably, the size of the peat soil particles is 1-14 meshes, and the water content of the peat soil is 50% -75%.
Preferably, the ciliate desert-grass seedling raising method is applied to seedling raising equipment, the seedling raising equipment comprises a box body and an atomizing spray head,
the step of performing moisture-preserving culture on the daughter plants in the plug tray comprises the following steps:
and putting the hole disc into the box body, and starting an atomizing nozzle arranged above the hole disc at intervals of 3-6 hours for spraying for 5-20 seconds each time so as to keep the humidity of the culture medium at 50-75%.
Preferably, the ciliate desert-grass seedling culture method is applied to seedling culture equipment, the seedling culture equipment comprises a box body and a heater,
the temperature-controlled culture of the plug tray comprises the following steps:
and putting the plug tray into the box body, and starting a heater to keep the temperature in the box body to be more than 18 ℃.
Preferably, the ciliate desert-grass seedling raising method is applied to seedling raising equipment, the seedling raising equipment comprises a box body and a light supplement lamp,
the light supplementing culture of the plug tray comprises:
and (3) putting the plug tray into the box body, and after the centipede grass plant grows out a green bud, starting a light supplement lamp, wherein the light supplement time is 6-10 hours each time, and the light period is 12-15 hours.
Preferably, after the steps of performing the moisturizing culture, the temperature-controlled culture and the light-supplemented culture on the plug tray, the method further comprises the following steps of:
when the green bud of the ciliate desert-grass grows to 3-5cm high, carrying out conventional seedling hardening and transplanting and field planting.
In addition, in order to achieve the above object, the present invention also provides a ciliate desert-grass seedling raising device, comprising:
the box body is internally provided with a support piece;
the plug is arranged on the support piece and used for placing the ciliate desert-grass seed plants and the culture medium;
environmental control system, including light filling device, water spraying system and heater, the light filling device is used for carrying out the light filling to the sub-trunk in the cave dish of putting into the incubator and cultivates, water spraying system includes atomizer, water spraying system is used for moisturizing the cultivation to the sub-trunk in the cave dish of putting into the incubator, the heater is used for carrying out the temperature-controlled cultivation to the sub-trunk in the cave dish of putting into the incubator.
The ciliate desert-grass seedling method and the equipment provided by the embodiment of the invention greatly improve the germination rate and the seedling rate of the sub-plants by sterilizing the sub-plants and providing a proper culture medium and a proper culture environment, thereby reducing the requirements on the germination nodes of the sub-plants for propagation, cutting more sub-plants for the rhizome of the same mother plant, saving propagation materials, greatly improving the utilization rate of the ciliate desert-grass mother plant materials, greatly improving the propagation coefficient, manually setting the culture environment to perform moisture-preserving culture, temperature-controlling culture and light-supplementing culture on the sub-plants, providing relatively good, stable and consistent environmental conditions of temperature, humidity, illumination, moisture and the like for the growth of the ciliate desert-grass sub-plants, being beneficial to greatly improving the germination rate and the seedling rate of the sub-plants, shortening the seedling time, and being beneficial to breaking through the seasonal limitation of the ciliate desert-grass sub-plants, annual production is realized.
Drawings
FIG. 1 is a schematic flow chart of an embodiment of a method for growing ciliate desert-grass seedlings according to the present invention;
FIG. 2 is a schematic structural diagram of a ciliate desert-grass seedling raising device according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a light supplementing device in a ciliate desert-grass seedling raising device according to the present invention;
FIG. 4 is a schematic bottom structure of the ciliate desert-grass seedling growing device of the present invention;
FIG. 5 is a diagram showing the growth state of the sprout of ciliate desert-grass after being cultivated for 20 days;
FIG. 6 is a diagram showing the state of seedling formation of ciliate desert-grass after 40 days of plant division cultivation.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The main solution of the embodiment of the invention is as follows: cutting a rhizome of a ciliate desert-grass mother plant into sub-plants, sequentially and respectively soaking the sub-plants with a carbendazim solution, a copper quinoline copper tebuconazole solution and an amino acid water soluble fertilizer, putting the sub-plants into a hole tray, covering the sub-plants in the hole tray with a culture medium, and performing moisturizing culture, temperature control culture and light supplement culture on the sub-plants in the hole tray.
The time suitable for the plant division and propagation is limited in the prior art, and is mainly concentrated in 5-6 months; the propagation coefficient of the plant division propagation is lower, and generally only 2-3 plants can be propagated by one stock plant, so that the propagation material waste is serious.
The invention provides a solution, which provides relatively good, stable and consistent environmental conditions such as temperature, humidity, illumination, moisture and the like for the growth of ciliate desert-grass seed plants, is beneficial to greatly improving the germination rate and the seedling rate of the seed plants, shortening the seedling time, and breaking through the seasonal limitation of ciliate desert-grass branch seedling culture to realize annual production, and experiments show that 20 days of ciliate desert-grass branch seedling culture shown in figure 5 shows that new buds emerge, 30 days of new bud emergence proportion reaches 92%, and 90% of seedling rate, 40 days of ciliate desert-grass branch seedling culture shown in figure 6 shows that the 40 days of seedling state graph, 20 days of new bud growth state graph of ciliate desert-grass branch seedling culture, 40 days of seedling hardening can be carried out, and the seedling period is shortened to about 40 days; moreover, as the germination rate and the seedling rate of the sub-plants are greatly improved, the requirements on the germination nodes of the sub-plants are reduced, more than 10 sub-plants can be separated from one mother plant, the plant division propagation coefficient is improved by more than 5 times, and the utilization rate of the ciliate desert-grass mother plant material is greatly improved; the seedling raising method is simple and convenient to operate, overcomes the defects of the prior art, has low requirements on the technology and skill of operators, has strong practicability, and is beneficial to realizing large-scale and intensive seedling raising of the ciliate desert-grass.
Referring to fig. 2, in an embodiment of the ciliate desert-grass seedling raising method of the present invention, the ciliate desert-grass seedling raising method includes:
step S10, cutting the rhizome of the ciliate desert-grass mother plant into sub-plants;
in this example, a growing ciliate desert-grass plant is selected as a ciliate desert-grass stock plant, the petioles, leaves and surrounding fine roots of the stock plant are cut off, the stock plant is washed clean with clear water, the root stock is cut off by a sharp blade, and the sub-plants are separated.
Preferably, the daughter strain is 1-5cm in length and/or comprises 1-3 germinating nodes.
In this embodiment, the rhizome is cut into rhizome segments with a length of 1-5cm and/or containing 1-3 germination nodes by a sharp blade, all the rhizome segments or part of the rhizome segments selected from all the rhizome segments are used as sub-plants, and the sub-plants cut out according to the length and/or the germination nodes provided by this embodiment can ensure a high seedling success rate, can utilize propagation materials to the maximum extent, and greatly improve the utilization rate of the centipede grass mother plant material.
Step S20, sequentially and respectively soaking the strains in a carbendazim solution, a copper quinoline copper tebuconazole copper preparation solution and an amino acid water-soluble fertilizer;
in this embodiment, the daughter plant is sequentially and respectively soaked in a carbendazim solution and a copper quinolincuptebuconazole preparation solution, the carbendazim solution and the copper quinolincuptebuconazole preparation solution are used for performing sterilization treatment on the daughter plant, the daughter plant is washed with clear water for 2-3 times, and then the daughter plant is soaked in an amino acid water-soluble fertilizer, and the amino acid water-soluble fertilizer is used for providing nutrients for the daughter plant.
Preferably, the concentration of the carbendazim solution is 1000-2000 times of the solution, and the soaking time is 5-30 minutes; the concentration of the quinoline copper tebuconazole copper preparation solution is 1000-2000 times of that of the solution, and the soaking time is 5-10 minutes; the concentration of the amino acid water-soluble fertilizer is 50-100 times of the concentration of the amino acid water-soluble fertilizer, and the soaking time is 5-10 minutes.
In this embodiment, it should be noted that the carbendazim solution is obtained by diluting carbendazim solid by 2000 times in 1000-fold, the copper quinolate-copper tebuconazole solution is obtained by diluting the copper quinolate-copper tebuconazole solution by 2000 times in 1000-fold, and the amino acid water-soluble fertilizer is obtained by diluting the amino acid water-soluble fertilizer solid by 50-100 times.
Specifically, the concentration of the carbendazim solution is 1000-2000 times of the solution, and the soaking time is 5-30 minutes; the concentration of the quinoline copper tebuconazole copper preparation solution is 1000-2000 times of that of the solution, and the soaking time is 5-10 minutes; the concentration of the amino acid water-soluble fertilizer is 50-100 times of that of the liquid, the soaking time is 5-10 minutes, the daughter is placed into the diluted carbendazim solution to be soaked for 5-30 minutes, the daughter is sterilized, then the daughter is taken out of the carbendazim solution, the diluted quinoline copper tebuconazole copper solution is placed into the soaked for 5-10 minutes, the daughter is sterilized, then the daughter is taken out of the quinoline copper tebuconazole copper solution and is washed with clear water for 2-3 times, the daughter is placed into the diluted amino acid water-soluble fertilizer to be soaked for 5-10 minutes, the daughter is fertilized, the daughter is taken out of the amino acid water-soluble fertilizer and is naturally air-dried for 10-30 minutes, the germination rate and the seedling rate of the daughter can be effectively improved by sterilizing and fertilizing the daughter, and the requirements on the length of the daughter and the number of sprouting nodes are further reduced, greatly improves the multiplication coefficient and the utilization rate of the parent plant material.
Step S30, placing the daughter plant into a plug tray, and covering the daughter plant in the plug tray with a culture substrate;
in this embodiment, the daughter plant is placed in the plug and covered with culture medium, specifically, after a single ciliate desert-grass daughter plant is placed in the plug, the prepared culture medium is covered, and the culture medium is kept not higher than the plug plane after covering the daughter plant.
Preferably, a single hole of the plug is in a square shape with the side length of 4-10cm or a round shape with the diameter of 4-10cm, and the depth is 5-10cm, so that a sufficient growth environment for the sub-plants is provided, meanwhile, the maximized space utilization is realized, and the number and the efficiency of propagation are increased;
preferably, the culture medium is a mixture of peat soil and hydrated lime with the mass ratio of 100 kg: 1-10 kg.
In the embodiment, peat soil and hydrated lime are uniformly mixed according to the mass ratio of 100 kg: 1-10kg, the obtained mixture is alkalescent, the mixture is used as a culture medium, the mass ratio is preferably properly adjusted within the mass ratio range of 100 kg: 1-10kg, the pH value of the mixture is 7-8, the cultivation of the ciliate desert-grass seed plants is most suitable, the culture medium suitable for acidity and alkalinity is favorable for improving the germination rate and the seedling rate of the ciliate desert-grass seed plants, the requirements on the length of the ciliate desert-grass seed plants and the number of germination nodes are further reduced, and the proliferation coefficient and the utilization rate of mother plant materials are greatly improved.
Preferably, the size of the peat soil particles is 1-14 meshes, and the water content of the peat soil is 50% -75%.
In this example, it should be noted that, peat soil with a particle size of 1-14 mesh and a water content of 50% -75% was selected as the raw material for preparing the culture substrate, the peat soil with the particle size of 1-14 meshes can be pretreated by crushing, sieving and other methods to obtain the peat soil with the particle size of 1-14 meshes as a raw material for preparing the culture medium, the peat soil with the water content of 50-75 percent can be pretreated by adding water, drying and other methods to obtain the peat soil with the water content of 50-75 percent as a raw material for preparing a culture medium, the appropriate particle size and water content of the peat soil are favorable for improving the germination rate and seedling rate of the strains, further reducing the requirements on the length of the sub-plants and the number of the sprouting nodes and greatly improving the multiplication coefficient and the utilization rate of the parent plant material.
And step S40, performing moisturizing culture, temperature-controlled culture and light-supplemented culture on the strains in the plug tray.
In this embodiment, it is specifically right that the seedling carries out the cultivation of moisturizing, accuse temperature and light filling in the cave dish, put into the environment of manual control with the cave dish that places the seedling and cultivate the matrix, carry out the cultivation of moisturizing in order to control the humidity environment of seedling in-process of the seedling, carry out the cultivation of accuse temperature in order to control the temperature environment of seedling in-process of the seedling, carry out the light filling cultivation in order to control the illumination environment of seedling in-process of the seedling, environmental conditions such as temperature, humidity, illumination and moisture that provide relatively good, stable and unanimous for the growth of ciliate desert grass seedling, both do benefit to improving the germination percentage and the percent of seedling of the seedling by a wide margin, shorten the time of growing seedlings, also be favorable to breaing through the seasonal restriction that ciliate grass divides the seedling, realize annual production.
Preferably, after the steps of performing moisture-preserving culture, temperature-controlling culture and light-supplementing culture on the plug tray, the method further comprises:
when the green bud of the ciliate desert-grass grows to 3-5cm high, carrying out conventional seedling hardening and transplanting and field planting.
Specifically, when the green bud of the ciliate desert-grass grows to 3-5cm high, the seedling raising process of the daughter plant is smoothly completed, and the obtained seedlings can be subjected to conventional seedling hardening and transplanting and field planting.
In the embodiment, the germination rate and the seedling rate of the sub-plants are greatly improved by sterilizing the sub-plants and providing a proper culture medium and a proper culture environment, so that the requirements on the germination nodes of the sub-plants for propagation expansion are reduced, more sub-plants can be cut off from the rhizome of the same mother plant, the propagation coefficient is greatly improved, the utilization rate of the pteris vittata mother plant material is greatly improved, the propagation material is saved, the culture environment is manually set for carrying out moisture-keeping culture, temperature-controlling culture and light-supplementing culture on the sub-plants, relatively good, stable and consistent environmental conditions such as temperature, humidity, illumination, moisture and the like are provided for the growth of the pteris vittata sub-plants, the germination rate and the seedling rate of the sub-plants are greatly improved, the seedling culture time is shortened, the seasonal limitation of the pteris vittata sub-plant seedling culture is broken through, and the annual production is realized.
Furthermore, the pteris vittata seedling growing method is applied to seedling growing equipment which comprises a box body, an atomizing nozzle, a heater and a light supplementing lamp,
the step of performing moisturizing culture on the strains in the plug tray comprises the following steps:
and putting the hole tray into the box body, and starting an atomizing nozzle arranged above the hole tray at intervals of 3-6 hours for spraying for 5-20 seconds each time so as to keep the humidity of the culture medium at 50% -75%.
In this embodiment, the humidity of the culture medium is controlled between 50% and 75% by spraying intermittently through the atomizer, so that the humidity of the environment and the water content of the culture medium can be uniformly increased, and the culture medium can be kept at the water content which is most suitable for the growth of the stock.
Preferably, the atomizing spray head is arranged above the arrangement position of the hole disk and is 30-60cm away from the hole disk below the atomizing spray head.
The step of temperature-controlled culture of the plug tray comprises the following steps:
and putting the plug tray into the box body, and starting a heater to keep the temperature in the box body to be more than 18 ℃.
In this example, the daughter plants were grown in an optimum temperature environment of 18 ℃ or higher by a heater.
Preferably, the heater is arranged at the bottom in the box body, a heating plate is arranged on the heater to enable heating to be more uniform, and the heater is connected with an external power supply and a control switch.
The step of performing light supplement culture on the plug comprises the following steps:
and (3) putting the plug tray into the box body, and after the centipede grass plant grows out a green bud, starting a light supplement lamp, wherein the light supplement time is 6-10 hours each time, and the light period is 12-15 hours.
In this embodiment, a light supplement lamp is used to provide suitable illumination for the green buds growing from the ciliate desert-grass plant.
Preferably, the light supplement lamp is an LED plant growth lamp or an incandescent lamp.
Preferably, the illumination intensity of the light supplement lamp is 2000-.
Preferably, the light supplement lamp is arranged above the arrangement position of the hole tray and is 30-80cm away from the hole tray below the light supplement lamp.
It is easy to understand that, in the step of performing the moisture-preserving culture, the temperature-controlling culture and the light-supplementing culture on the daughter plants in the plug tray, the moisture-preserving culture, the temperature-controlling culture and the light-supplementing culture are performed at respective time schedules, and may be performed independently or simultaneously, and there is no sequence.
Further, another embodiment of the present invention provides a pteris vittata seedling raising apparatus, referring to fig. 2 to 4, including:
the box body 1 is internally provided with a supporting piece;
the plug 6 is arranged on the support piece, and the plug 6 is used for placing the ciliate desert-grass seed plant and the culture substrate;
environmental control system, including light filling device, water-spraying system and heater 11, the light filling device is used for carrying out the light filling to the sub-trunk in the cave dish of putting into the incubator and cultivates, water-spraying system includes atomizer 2, water-spraying system is used for moisturizing the sub-trunk in the cave dish 6 of putting into box 1 and cultivates, heater 11 is used for carrying out the accuse temperature to the sub-trunk in the cave dish 6 of putting into box 1 and cultivates.
Specifically, referring to fig. 2, in one embodiment, the ciliate desert-grass seedling raising device is a multi-layer incubator comprising a cultivation support system and an environment control system; the cultivation supporting system comprises a box body 1, a plug tray 6 and a supporting piece, wherein the supporting piece comprises a supporting plate 7 and a fixing column 8, the fixing column 8 is fixed at the same height of the inner side surface of the box body 1, the supporting plate 7 is arranged on the fixing column 8, and the plug tray 6 is arranged on the supporting plate 7; the environment control system comprises a light supplementing device, a spraying system, a heating plate 9 and a water collecting and draining device, and specifically, referring to fig. 3, the light supplementing device consists of a light supplementing protective sleeve 3, a light supplementing lamp holder 4 and a light supplementing lamp 5, the light supplementing protective sleeve 3 and the light supplementing lamp holder 4 are fixed below the supporting plate 7 and the top of the box body 1, and the light supplementing lamp 5 is detachably connected with the light supplementing lamp holder; the spraying system comprises an atomizing spray head 2 and a water supply pipe (not shown in the figure), the water supply pipe is used for being connected with an external tap water supply pipeline and a control device, and the atomizing spray head 2 is arranged below the supporting plate 7 and the top of the box body 1; the heating plate 9 is arranged at the bottom in the box body 1, specifically, referring to fig. 4, a heater 11 is arranged below the heating plate 9, the heater 11 is connected with an external power supply and a control switch, the water collecting and discharging device comprises a water collecting tank 10 and a water discharging hole 12, the water collecting tank 10 is arranged at the edge of the heating plate 9 and is lower than the heating plate 9, and the water discharging hole 12 is connected with an external water discharging pipeline.
In the embodiment, through the ciliate desert-grass seedling raising equipment, relatively good, stable and consistent environmental conditions such as temperature, humidity, illumination, moisture and the like are provided for growth of ciliate desert-grass seeds, so that the germination rate and the seedling rate of the ciliate desert-grass seeds are greatly improved, the seedling raising time is shortened, seasonal limitation of seedling raising of ciliate desert-grass seeds in separate plants is broken through, and annual production is realized.
Those skilled in the art will appreciate that the device configurations illustrated in fig. 2-4 are not meant to be limiting of the device and may include more or less components than those illustrated, or some components may be combined, or a different arrangement of components.
Optionally, a single hole of the plug tray 6 is in a square shape with a side length of 4-10cm, or in a circular shape with a diameter of 4-10cm, and the depth is 5-10 cm;
optionally, the light supplement lamp 5 is an LED plant growth lamp or an incandescent lamp;
optionally, the illumination intensity of the light supplement lamp 5 is 2000-;
optionally, the distance between the light supplement lamp 5 and the plug tray 6 below the light supplement lamp is 30-80 cm;
optionally, the distance between the atomizing nozzle 2 and the tray 6 below the atomizing nozzle is 30-60 cm.
Further, the pteris vittata seedling raising device can be applied to various embodiments of the pteris vittata seedling raising method, and particularly, one structural implementation form of the pteris vittata seedling raising device is a multilayer incubator which can be suitable for the division, propagation and cultivation of pteridophyte.
The multi-layer incubator comprises a plurality of cultivation substrates, a plurality of cultivation substrates and a plurality of layers of incubators, wherein the cultivation substrates cover the cultivation substrates after the single centipede grass seeds are placed in the plug trays, and then the plug trays are placed in the multi-layer incubator for moisture preservation, temperature control and light supplement cultivation;
optionally, the quality of the covering base is not higher than the plane of the plug;
optionally, the moisturizing culture is sprayed for 1 time every 3-6 hours, and each time is sprayed for 5-20 seconds; maintaining the humidity of the culture medium at 50-75%;
optionally, the temperature-controlled culture comprises timely starting a heater to keep the temperature in the box body above 18 ℃;
optionally, the light supplementing culture comprises turning on a light supplementing lamp after the centipede grass seed plants grow green buds, wherein the light supplementing time is 6-10 hours each time, and the light cycle is 12-15 hours;
optionally, the growing height of the green bud of ciliate desert-grass reaches 3-5cm, and the ciliate desert-grass can be moved out of the multilayer incubator for conventional seedling hardening and transplanting.
Further, another embodiment of the present invention provides a ciliate desert-grass seedling raising method, including the following steps:
digging strong ciliate desert-grass as a stock plant, cutting off petioles, leaves and surrounding fine roots, and washing with clear water; then cutting off the rhizome of the ciliate desert-grass mother plant by a sharp blade to separate out sub-plants; the length of the cut rhizome is 1cm, and the cut rhizome comprises 1 germination node.
Step two, sterilizing the strains, namely soaking the strains in a carbendazim solution, a copper quinoline copper tebuconazole solution and an amino acid water-soluble fertilizer in sequence; the concentration of the carbendazim solution is 1000 times of the carbendazim solution, and the time for soaking the strains is 5 min; the concentration of the quinoline copper tebuconazole copper preparation solution is 1000 times of the solution, and the time for soaking the strains is 5 min; the concentration of the amino acid water-soluble fertilizer is 100 times of the solution, and the time for soaking the strains is 10 min. Washing the seed plants with clear water for 3 times before soaking the seed plants with amino acid water soluble fertilizer, and naturally air drying for 30min after soaking the seed plants.
Step three, the culture substrate is a mixture of peat soil and hydrated lime, the size of peat soil particles is 1-7 meshes, and the water content is 50-60%; the peat soil and the hydrated lime are uniformly mixed according to the mass ratio of 100kg to 1kg, and the pH value of the culture substrate is 7.0.
And step four, placing the single ciliate desert-grass seed plant into a plug tray, covering a culture medium, and placing the plug tray into ciliate desert-grass seedling raising equipment for moisturizing, temperature control and light supplement culture. The quality of the covering base is not higher than the plane of the plug.
The ciliate desert-grass seedling raising equipment is a multilayer incubator and comprises a cultivation supporting system and an environment control system. Cultivate braced system and include box, cave dish and support piece, support piece includes backup pad and fixed column, and the fixed column is fixed in the same height of box medial surface, and on the fixed column was arranged in to the backup pad, the cave dish was arranged in the backup pad. The environment control system comprises a light supplementing device, a spraying system, a heating plate and a water collecting and draining device, the light supplementing device consists of a light supplementing protective sleeve, a light supplementing lamp holder and a light supplementing lamp, the light supplementing protective sleeve and the light supplementing lamp holder are fixed below the supporting plate and the top of the box body, and the light supplementing lamp is detachably connected with the light supplementing lamp holder; the spraying system comprises an atomizing spray head and a water supply pipe, the water supply pipe is connected with an external tap water supply pipeline and a control device, and the atomizing spray head is arranged below the supporting plate and the top of the box body; the heating plate is arranged at the bottom in the box body, a heater is arranged on the heating plate, and the heater is connected with an external power supply and a control switch; the water collecting and draining device comprises a water collecting tank and a water draining hole, the water collecting tank is arranged at the edge of the heating plate and is lower than the heating plate, and the water draining hole is connected with an external water draining pipeline. The single hole of the plug is in a circular shape with the diameter of 4cm and the depth of 5 cm; the light supplement lamp is an LED plant growth lamp, the illumination intensity is 2000Lux, and the distance between the light supplement lamp and the hole tray below the light supplement lamp is 30 cm; the distance between the atomizing spray head and the hole disc below the atomizing spray head is 30 cm.
The multi-layer incubator for moisturizing culture comprises starting the atomizer to spray once every 6 hours, wherein the spraying time is 20 seconds, and the humidity of the culture medium is kept at 65-75%.
The multi-layer incubator for temperature-controlled cultivation comprises a heater which is started in due time and the temperature in the incubator is kept above 18 ℃.
The light supplementing cultivation of the multilayer incubator comprises the step of starting a light supplementing lamp after the centipede grass plant grows out a green bud, wherein the light supplementing time is 6 hours each time, and the light period is 12 hours. The green buds of ciliate desert-grass can grow to 3-5cm high, and then can be moved out of the multilayer incubator for conventional seedling hardening and transplanting and planting.
Further, another embodiment of the present invention provides a ciliate desert-grass seedling raising method, including the following steps:
referring to fig. 1, 2 and 3, the present embodiment provides a method for cultivating ciliate desert-grass by dividing plant, comprising the steps of separating sub-plants from ciliate desert-grass mother plant, sterilizing the sub-plants, applying fertilizer, preparing cultivation medium and cultivating in a multi-layer incubator.
Digging strong ciliate desert-grass as a stock plant, cutting off petioles, leaves and surrounding fine roots, and washing with clear water; then cutting off the rhizome of the ciliate desert-grass mother plant by a sharp blade to separate out sub-plants; the cut rhizome length is 5cm, and the cut rhizome contains 3 germination nodes.
Step two, sterilizing the strains, namely soaking the strains in a carbendazim solution, a copper quinoline copper tebuconazole solution and an amino acid water-soluble fertilizer in sequence; the concentration of the carbendazim solution is 2000 times of the carbendazim solution, and the time for soaking the strains is 30 min; the concentration of the quinoline copper tebuconazole copper preparation solution is 2000 times of the solution, and the time for soaking the strains is 10 min; the concentration of the amino acid water-soluble fertilizer is 50 times of the solution, and the time for soaking the strains is 5 min. Washing the seed plants with clear water for 2 times before soaking the seed plants in amino acid water soluble fertilizer, and naturally air drying for 10min after soaking the seed plants.
Step three, the culture substrate is a mixture of peat soil and hydrated lime, the size of peat soil particles is 7-14 meshes, and the water content is 60-70%; the peat soil and the hydrated lime are uniformly mixed according to the mass ratio of 100kg to 10kg, and the pH value of the culture substrate is 8.0.
And step four, placing the single ciliate desert-grass seed plant into a plug tray, covering a culture medium, and placing the plug tray into ciliate desert-grass seedling raising equipment for moisturizing, temperature control and light supplement culture. The quality of the covering base is not higher than the plane of the plug.
The ciliate desert-grass seedling raising equipment is a multilayer incubator and comprises a cultivation supporting system and an environment control system. Cultivate braced system and include box, cave dish and support piece, support piece includes backup pad and fixed column, and the fixed column is fixed in the same height of box medial surface, and on the fixed column was arranged in to the backup pad, the cave dish was arranged in the backup pad. The environment control system comprises a light supplementing device, a spraying system, a heating plate and a water collecting and draining device, the light supplementing device consists of a light supplementing protective sleeve, a light supplementing lamp holder and a light supplementing lamp, the light supplementing protective sleeve and the light supplementing lamp holder are fixed below the supporting plate and the top of the box body, and the light supplementing lamp is detachably connected with the light supplementing lamp holder; the spraying system comprises an atomizing spray head and a water supply pipe, the water supply pipe is connected with an external tap water supply pipeline and a control device, and the atomizing spray head is arranged below the supporting plate and the top of the box body; the heating plate is arranged at the bottom in the box body, a heater is arranged on the heating plate, and the heater is connected with an external power supply and a control switch; the water collecting and draining device comprises a water collecting tank and a water draining hole, the water collecting tank is arranged at the edge of the heating plate and is lower than the heating plate, and the water draining hole is connected with an external water draining pipeline. A single hole of the hole disc is in a square shape with the side length of 10cm and the depth of 10 cm; the light supplement lamp is an LED plant growth lamp, the illumination intensity is 5000Lux, and the distance between the light supplement lamp and the hole tray below the light supplement lamp is 80 cm; the distance between the atomizing spray head and the tray below the atomizing spray head is 60 cm.
The multi-layer incubator is sprayed for 5 seconds 1 time at intervals of 3 hours while maintaining the humidity of the culture medium at 50-65%.
The multi-layer incubator for temperature-controlled cultivation comprises a heater which is started in due time and the temperature in the incubator is kept above 18 ℃.
The light supplementing cultivation of the multilayer incubator comprises the step of starting a light supplementing lamp after the centipede grass plant grows out a green bud, wherein the light supplementing time is 10 hours each time, and the light period is 12 hours. The green buds of ciliate desert-grass can grow to 3-5cm high, and then can be moved out of the multilayer incubator for conventional seedling hardening and transplanting and planting.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, or article that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, or article. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, or article that comprises the element.
The above-mentioned serial numbers of the embodiments of the present invention are merely for description and do not represent the merits of the embodiments.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (10)

1. A ciliate desert-grass seedling growing method is characterized by comprising the following steps:
cutting the rhizome of the ciliate desert-grass mother plant into sub-plants;
sequentially and respectively soaking the strains in a carbendazim solution, a copper quinoline copper tebuconazole solution and an amino acid water-soluble fertilizer;
placing the daughter plant into a plug tray, and covering the daughter plant in the plug tray with a culture medium;
and performing moisture preservation culture, temperature control culture and light supplement culture on the strains in the plug tray.
2. A method of growing seedlings of ciliate desert-grass according to claim 1, wherein the length of the sub-plant is 1-5cm and/or contains 1-3 germination nodes.
3. The ciliate desert-grass seedling raising method according to claim 1, wherein the concentration of the carbendazim solution is 1000-2000 times of the solution, and the soaking time is 5-30 minutes; the concentration of the quinoline copper tebuconazole copper preparation solution is 1000-2000 times of that of the solution, and the soaking time is 5-10 minutes; the concentration of the amino acid water-soluble fertilizer is 50-100 times of the concentration of the amino acid water-soluble fertilizer, and the soaking time is 5-10 minutes.
4. A method for growing seedlings of ciliate desert-grass as claimed in claim 1, wherein the culture medium is a mixture of peat soil and hydrated lime in a mass ratio of 100 kg: 1-10 kg.
5. A method for growing ciliate desert-grass seedlings according to claim 4, wherein the size of the peat soil particles is 1-14 meshes, and the moisture content of the peat soil is 50% -75%.
6. The ciliate desert-grass seedling raising method according to claim 1, which is applied to seedling raising equipment comprising a box body and an atomizing nozzle,
the step of performing moisturizing culture on the strains in the plug tray comprises the following steps:
and putting the hole tray into the box body, and starting an atomizing nozzle arranged above the hole tray at intervals of 3-6 hours for spraying for 5-20 seconds each time so as to keep the humidity of the culture medium at 50% -75%.
7. The ciliate desert-grass seedling raising method according to claim 1, which is applied to seedling raising equipment comprising a box body and a heater,
the step of temperature-controlled culture of the plug tray comprises the following steps:
and putting the plug tray into the box body, and starting a heater to keep the temperature in the box body to be more than 18 ℃.
8. The ciliate desert-grass seedling raising method according to claim 1, which is applied to seedling raising equipment, wherein the seedling raising equipment comprises a box body and a light supplement lamp,
the step of performing light supplement culture on the plug comprises the following steps:
and (3) putting the plug tray into the box body, and after the centipede grass plant grows out a green bud, starting a light supplement lamp, wherein the light supplement time is 6-10 hours each time, and the light period is 12-15 hours.
9. A method for growing ciliate desert-grass seedlings according to any one of claims 1 to 8, wherein after the steps of performing moisture-preserving culture, temperature-controlling culture and light-supplementing culture on the plug tray, the method further comprises the following steps:
when the green bud of the ciliate desert-grass grows to 3-5cm high, carrying out conventional seedling hardening and transplanting and field planting.
10. The utility model provides a ciliate desert grass equipment of growing seedlings which characterized in that, ciliate desert grass equipment of growing seedlings includes:
the box body is internally provided with a support piece;
the plug is arranged on the support piece and used for placing the ciliate desert-grass seed plants and the culture medium;
environmental control system, including light filling device, water-spraying system and heater, the light filling device is used for carrying out the light filling to the sub-trunk in the cave dish of putting into the box and cultivates, water-spraying system includes atomizer, water-spraying system is used for moisturizing the cultivation to the sub-trunk in the cave dish of putting into the box, the heater is used for carrying out the accuse temperature cultivation to the sub-trunk in the cave dish of putting into the box.
CN202111097302.1A 2021-09-18 2021-09-18 Grateloupia filicina seedling raising method and equipment Pending CN113767764A (en)

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