CN105409565A - Industrialized seedling method for super-enriched plant herb of Chinese brake - Google Patents
Industrialized seedling method for super-enriched plant herb of Chinese brake Download PDFInfo
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- CN105409565A CN105409565A CN201510937385.9A CN201510937385A CN105409565A CN 105409565 A CN105409565 A CN 105409565A CN 201510937385 A CN201510937385 A CN 201510937385A CN 105409565 A CN105409565 A CN 105409565A
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- seedling
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- ciliate desert
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
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Abstract
The present invention relates to an industrialized seedling method for super-enriched plant herb of Chinese brake. The method mainly comprises the parts of: mixing a seedling matrix; placing the seedling matrix into a tray; seeding spore powder of herb of Chinese brake; maintaining herb of Chinese brake in the prothallium stage; and maintaining herb of Chinese brake in the sporophore stage. In order to solve the difficulty that the spore volume of the spore seedling of herb of Chinese brake is small and the planting amount is difficult to control, the seedling matrix and a spore powder seeding technology are designed, so that the defect that spore seedling is hard for industrialized operation is solved; In addition, the management of an initial seedling stage of the prothallium stage and the sporophore stage is designed for the specific requirements of moisture and temperature in the seedling stage of spore of herb of Chinese brake . The method can be taken as an industrialized seedling method for super-enriched seedlings of herb of Chinese brake.
Description
Technical field
The present invention relates to heavy metal super-enriched plant seedling from spore method, the particularly factorial seedling-culturing method of the super enriching plant ciliate desert-grass of Pteridaceae Pteris, specifically a kind ofly meets arsenic super enriching plant ciliate desert-grass prothallium and sprouts and the method managed and protected of sporophyte Seedling Stage.
Background technology
Heavy metal pollution of soil problem is one of puzzlement global environment problem, utilizes heavy metal super-enriched plant can remove pollutant in soil.Along with super enriching plant extensive use, day by day urgent to the demand of super enriching plant seedling, thus the exploration of super enriching plant seedling breeding method is very active.
Arsenic super enriching plant ciliate desert-grass applies the maximum super enriching plant kind of area at present, and this plant belongs to Pteridaceae Pteris plant, is bred by spore.Find because ciliate desert-grass adopts sporogenesis under field conditions (factors) in engineering practice, its growth rate is too slow, is difficult to the demand meeting practical application.Although tissue cultures is a kind of feasible ciliate desert-grass quick propagating technology, but it costly and complicated operation, how fast, economical and cultivate ciliate desert-grass seedling easily on a large scale, thus reduction rehabilitation cost, remain As polluted soil phytoremediation engineering and apply middle urgent need to solve the problem.
Summary of the invention
The object of the present invention is to provide and a kind ofly can be used for the method that super enriching plant ciliate desert-grass carries out factorial seedling growth.The method can ensure that ciliate desert-grass prothallium is sprouted and condition needed for sporophyte seedling growth, and is convenient to factorial seedling growth operation.Breed the demand of acid-base value, porosity, moisture holding capacity and special dietary material to seedling medium according to ciliate desert-grass spore, devise factorial seedling-culturing method.Consider that spore volume is little, be difficult to the difficulty of sowing, devise the method adopting and carried out by spore suspension sowing in liquid, to control seeding quantity.For ease of the thinning of ciliate desert-grass sporophyte and the transplanting in later stage, method proposes optimized thickness of sowing, and the seedling dish size matched with it.The method can be soil remediation and provides large batch of super enriching plant seedling.Operating process of the present invention is simple, the super enriching plant seedling neat specification of breeding and healthy and strong, is convenient to later stage super enriching plant and extracts heavy metal in soil.
A kind of super enriching plant ciliate desert-grass factorial seedling-culturing method provided by the invention, specifically comprises the following steps:
The first step, by meeting the seedling medium formula of super enriching plant ciliate desert-grass seedling from spore requirement, is mixed by batch mixer, forms ciliate desert-grass seedling medium;
Second step, the seedling medium to first step gained adds the seedling-cultivating tray of applicable ciliate desert-grass seedling from spore, gently presses, and makes the gas porosity of seedling medium meet ciliate desert-grass seedling from spore demand;
3rd step, is sown in the seedling-cultivating tray surface that seedling medium is housed by ciliate desert-grass spore by conidial powder sower;
4th step, is placed on cultivation platform by the seedling medium seedling-cultivating tray having broadcasted sowing conidial powder that is equipped with of the 3rd step gained;
5th step, the cultivation platform that seedling-cultivating tray is housed to the 4th step gained is positioned over greenhouse;
6th step, regularly carries out nebulizing spray to the seedling medium surface of broadcasting sowing spore, ensures ciliate desert-grass seedling from spore desired moisture level;
7th step, after 4 to June, spore, after prothallium to enter sporophyte period period, starts artificial thinning;
8th step, proceeds nebulizing spray and irrigates, transplant to land for growing field crops after treating sporophyte plant height 20 centimeters after thinning.
Super enriching plant ciliate desert-grass factorial seedling-culturing method of the present invention, compared with traditional seedling-cultivating method, more meets the demand of the sprouting of ciliate desert-grass prothallium and sporophyte primary growth, and can meet factorial seedling growth operation.Seedling medium of the present invention is containing the required basic nutrient condition of plant growth, and acid-base value controls between pH6 ~ pH8, and sprouts necessary nutrient element calcium containing ciliate desert-grass prothallium, and its concentration controls between 0.1% ~ 2%.Sprout and sporophyte seedling growth for adapting to ciliate desert-grass prothallium, seedling medium porosity is between 40% ~ 50%.For ensureing that factorial seedling growth product is convenient to transplant, seedling disk hole cave of the present invention diameter is between 2 ~ 8cm, and thinning density domination is at every cave 1 ~ 2 strain sporophyte.For controlling seedling from spore seeding quantity, type of seeding of the present invention adopts and is sowed in liquid by spore suspension, and the thickness of sowing of spore is in 10 ~ 20/hole.For ensureing that ciliate desert-grass prothallium is sprouted and sporophyte primary growth, seedling from spore temperature of the present invention controls between 25 DEG C to 30 DEG C, and after irrigating, water content of substrate reaches 80%.
Compared with prior art, its remarkable advantage: (1) improves on ciliate desert-grass prothallium germination rate, by using prothallium to sprout necessary nutrient calcium constituent, shortens prothallium period in the present invention; (2) improve factorial seedling growth sowing operating efficiency, propose the method adopting liquid sowing spore; (3) ciliate desert-grass seedling prepared by the present invention is applied, have the advantages that seedling is neat and healthy and strong, the soil remediation work in later stage can be directly used in, remove for utilizing plant a kind of method that heavy metal-polluted soil provides super enriching plant factorial seedling growth, there is good application prospect and economic benefit.
Accompanying drawing explanation
Fig. 1 is that super enriching plant ciliate desert-grass factorial seedling growth prothallium sprouts experimental result schematic diagram;
Fig. 2 increases ciliate desert-grass germination rate experimental result schematic diagram for adding calcium;
Fig. 3 shortens ciliate desert-grass prothallium experimental result in period schematic diagram for adding calcium.
Embodiment
Below by embodiment, the present invention is specifically described
embodiment 1prepared by super enriching plant ciliate desert-grass seedling medium
Gather agricultural land soil 5kg, its acid-base value is pH6.2, and nutrient content is full nitrogen 0.09%, full phosphorus 0.12%, full potassium 1.45%, and the content of organic matter is 15.9%.Shakeout by soil, thickness is no more than 2cm, and as shady and cool ventilation, place is air-dry.The air-dry degree to pulverizing easily, mechanically by the clods breaking in soil, crosses 1mm nylon mesh.Add 6g nitrate of lime to by the soil after sieving, and 1 kilogram of vermiculite fully mixes.
embodiment 2super enriching plant ciliate desert-grass conidial powder is sowed
The ciliate desert-grass seedling medium of vegetation is loaded 32(8 × 4) Miao Panzhong of hole gauge lattice, make seedling medium fill up seedling disk hole cave, flush with hole upper limb.By the compacting gently of surface, seedling disk hole cave, make seedling medium lower than seedling disk hole cave upper limb about 0.5cm.Take 1g ciliate desert-grass conidial powder, pour in 2L water, stir, vegetation becomes spore suspension.The spore suspension of preparation is loaded in the device that can spray, is broadcast by the water having sprayed conidial powder.By the regulation and control of injection flow rate, make the spore quantity in every hole between 10 ~ 20.
embodiment 3super enriching plant ciliate desert-grass is managed and protected seedling stage
The seedling medium sowing spore is put into greenhouse together with seedling dish, and the temperature in greenhouse controls between 25 DEG C to 30 DEG C.Nurturing period, when namely the water content of seedling medium irrigates before lower than 60%, deficit irrigation controls about 80%.
About after 4 months, ciliate desert-grass sporophyte is formed.When sporophyte height is 2cm to 5cm, carry out thinning.After thinning, each hole only has 2 strain sporophyte seedling.Continue after thinning to keep said temperature and damp condition.About after 5 months, it is high that sporophyte grows to 20cm, can transplant to field.
Should be understood that above-described embodiment only for technical conceive of the present invention and feature are described, its object is to understand content of the present invention for those skilled in the art and implement according to this, not embodiment is exhaustive, can not limit the scope of the invention with this.All technical schemes according to the present invention are modified or equivalent replacement, and do not depart from aim and the scope of technical solution of the present invention, and it all should be encompassed in the middle of right of the present invention.
Claims (9)
1. a super enriching plant ciliate desert-grass factorial seedling-culturing method, comprises the following steps:
The first step, by meeting the seedling medium formula of super enriching plant ciliate desert-grass seedling from spore requirement, is mixed by batch mixer, forms ciliate desert-grass seedling medium;
Second step, adds the seedling-cultivating tray of applicable ciliate desert-grass seedling from spore, gently presses in the seedling medium of first step gained, makes the gas porosity of seedling medium meet ciliate desert-grass seedling from spore demand;
3rd step, is sown in the seedling-cultivating tray surface that seedling medium is housed by ciliate desert-grass spore by conidial powder sower;
4th step, is placed on cultivation platform by the seedling medium seedling-cultivating tray having broadcasted sowing conidial powder that is equipped with of the 4th step gained;
5th step, the cultivation platform that seedling-cultivating tray is housed to the 4th step gained is positioned over greenhouse;
6th step, regularly carries out nebulizing spray to the seedling medium surface of broadcasting sowing spore, ensures ciliate desert-grass seedling from spore desired moisture level;
7th step, after 4 to June, spore, after prothallium to enter sporophyte period period, starts artificial thinning;
8th step, proceeds nebulizing spray and irrigates, transplant to land for growing field crops after treating sporophyte plant height 20 centimeters after thinning.
2. according to preparation method according to claim 1, it is characterized in that, described matrix acid-base value controls between pH6 ~ pH8.
3. according to preparation method according to claim 1, it is characterized in that, described matrix controls between 0.1% ~ 2% containing calcium concentration.
4. according to preparation method according to claim 1, it is characterized in that, described seedling medium total porosity is between 40% ~ 50%.
5. according to preparation method according to claim 1, it is characterized in that, described seedling disk hole cave diameter is between 2 ~ 8cm.
6. according to preparation method according to claim 1, it is characterized in that, spore suspension is sowed in liquid.
7. according to preparation method according to claim 1, it is characterized in that, the thickness of sowing of spore is in 10 ~ 20/hole.
8. according to preparation method according to claim 1, it is characterized in that, prothallium nebulizing spray in period is irrigated rear soil moisture content and is reached 80%.
9. according to preparation method according to claim 1, it is characterized in that, thinning density domination is at every cave 1 ~ 2 strain sporophyte.
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| CN201510937385.9A CN105409565A (en) | 2015-12-15 | 2015-12-15 | Industrialized seedling method for super-enriched plant herb of Chinese brake |
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| CN201510937385.9A CN105409565A (en) | 2015-12-15 | 2015-12-15 | Industrialized seedling method for super-enriched plant herb of Chinese brake |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613160A (en) * | 2016-11-23 | 2017-05-10 | 云南云投生态环境科技股份有限公司 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
| CN111357578A (en) * | 2020-04-30 | 2020-07-03 | 上海交通大学 | Method for improving germination and seedling success rate of arsenic hyper-enrichment plant ciliate desert-grass spores |
| CN113068568A (en) * | 2021-03-16 | 2021-07-06 | 西南林业大学 | Soil for quickly cultivating ciliate desert-grass, cultivation method and application |
| CN113767764A (en) * | 2021-09-18 | 2021-12-10 | 深圳文科园林股份有限公司 | Grateloupia filicina seedling raising method and equipment |
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| JPH09294463A (en) * | 1996-05-08 | 1997-11-18 | Minoru Fujii | Planting material for plant and cultivation of plant |
| CN1633834A (en) * | 2003-12-26 | 2005-07-06 | 中国科学院生态环境研究中心 | Method for promoting plant restoration efficiency of arsenic polluted soil |
| CN102119656A (en) * | 2010-11-10 | 2011-07-13 | 天津滨海国际花卉科技园区股份有限公司 | Bird nest fern spore germination breeding method |
| CN102715082A (en) * | 2012-07-16 | 2012-10-10 | 河南城建学院 | Efficient arsenic-absorbed ecological ciliate desert-grass screening method |
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2015
- 2015-12-15 CN CN201510937385.9A patent/CN105409565A/en active Pending
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| JPH09294463A (en) * | 1996-05-08 | 1997-11-18 | Minoru Fujii | Planting material for plant and cultivation of plant |
| CN1633834A (en) * | 2003-12-26 | 2005-07-06 | 中国科学院生态环境研究中心 | Method for promoting plant restoration efficiency of arsenic polluted soil |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613160A (en) * | 2016-11-23 | 2017-05-10 | 云南云投生态环境科技股份有限公司 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
| CN111357578A (en) * | 2020-04-30 | 2020-07-03 | 上海交通大学 | Method for improving germination and seedling success rate of arsenic hyper-enrichment plant ciliate desert-grass spores |
| CN113068568A (en) * | 2021-03-16 | 2021-07-06 | 西南林业大学 | Soil for quickly cultivating ciliate desert-grass, cultivation method and application |
| CN113767764A (en) * | 2021-09-18 | 2021-12-10 | 深圳文科园林股份有限公司 | Grateloupia filicina seedling raising method and equipment |
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Application publication date: 20160323 |