CN1633834A - Method for promoting plant restoration efficiency of arsenic polluted soil - Google Patents
Method for promoting plant restoration efficiency of arsenic polluted soil Download PDFInfo
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- CN1633834A CN1633834A CN 200310123407 CN200310123407A CN1633834A CN 1633834 A CN1633834 A CN 1633834A CN 200310123407 CN200310123407 CN 200310123407 CN 200310123407 A CN200310123407 A CN 200310123407A CN 1633834 A CN1633834 A CN 1633834A
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Abstract
The invention relates to a method for plant restoring soil of arsenic contamination, which is realized through inoculating arbuscular mycorrhizal fungi to centipedefern, a kind of pteridophyte, thus producing fungus root, the method comprises the steps of, (1) collecting raw pteridophyte material, obtaining spores of pteridophytes, cultivating young plant sprout, (2) producing fungus roots from pteridophyte, (3) transplanting the adult strain of young sprouts to arsenic contaminated soil. The invention can be applied to aid the arsenic super rich centipedefern to adapt to arsenic contamination environment.
Description
Technical field
The invention belongs to environmental protection technical field, relate to the pollution ecology recovering technology, be specifically related to the method for As polluted soil phytoremediation.
Background technology
Because industrial production activities such as geologic origin or arsenic minerals exploitation, smelting processing produce large tracts of land by the soil of arsenic contamination.Arsenic contamination not only causes crop yield to descend, and causes the farmland discarded under the serious situation, and the arsenic in the soil may enter human body by food chain or water body, thereby jeopardizes domestic animal and the resident is healthy, even causes malignant event such as resident colony poisoning.In the serious area of arsenic contamination (high-As area), because the plant growing difficulty, the natural landscape and the ecological balance are all suffered destruction in various degree, and phenomenon appears falling into disuse in some villages, and its loss is difficult to estimate.Therefore, the improvement of As polluted soil becomes one of important process content of ecotope worker with reparation.
The improvement of As polluted soil for a long time has special difficulty always.The chemical behavior of arsenic is bordering on metal, can not be degraded.In case enter the soil body just can only in soil clay particle fix, perhaps move, diffuse to body of groundwater, or enter food chain by plant absorbing.By applying the validity that certain chemical substance may reduce arsenic in the soil, but can not fundamentally eliminate the potential hazard of arsenic.
After the calendar year 2001 scientist found a kind of special plant, people had seen the hope of fundamentally repairing As polluted soil finally.This plant species is the pteridophyte ciliate desert-grass.It not only has superpower absorbing enriched ability to arsenic, and biomass is big, and is strong to adaptive capacity to environment, thereby may extract arsenic excessive in the soil by its absorption, reaches the purpose that purifies soil.Laboratory and field test show that all ciliate desert-grass has the potentiality of efficient reparation As polluted soil.
Yet using ciliate desert-grass reparation As polluted soil also has some inevitable limitation.At first, ciliate desert-grass is a pteridophyte, and early growth speed is slower; Secondly, too high arsenic concentration can suppress the ciliate desert-grass growth in the soil; Moreover under the situation of soil depletion or combined pollution, the growth of ciliate desert-grass often maybe can not be obtained enough essential mineral nutrients because of the metal murder by poisoning and is restricted.
As everyone knows, arbuscular mycorrhizal fungi can be in several ways or approach influence physiological metabolism and the growth and development process of plant.It is many that to studies show that arbuscular mycorrhiza plays a part in the mineral nutrition of plant (especially phosphorus nutrition) and stress physiology (as drought resisting, anti-salt, preventing from heavy metal, disease-resistant etc.) very important.Under the increasingly serious background of resource and environmental problem, the mycorhiza technology is as an effective and feasible biological pathway, can excavate biological self potentiality, improve the utilize ability of plant to natural resources, thereby cut down the consumption of energy, alleviate the pressure that environment bears, thereby wide application prospect is arranged.
On the other hand, our field investigation shows that the ciliate desert-grass of self-sow forms flourishing mycorhiza structure (seeing accompanying drawing 1) usually on arsenic and the heavy metal pollution area.The foundation of mycorrhizas homobium may adapt to contaminated environment for ciliate desert-grass and have special meaning.Prove that by constructed experiment the inoculation mycorrhizal fungi can further be improved the patience of ciliate desert-grass to arsenic.Barren and have under the situation of other metal combined contamination at nutrient, mycorhiza has significantly improved the ciliate desert-grass mineral nutrition and has promoted plant strain growth.Therefore, introduce the success of mycorrhizal fungi, quicken repair process and have positive role for the As polluted soil phytoremediation.
Summary of the invention
In view of the foregoing, the present invention proposes to repair As polluted soil in conjunction with using mycorhiza technology and ciliate desert-grass.
The objective of the invention is to auxiliary arsenic super enriching plant ciliate desert-grass and adapt to the special living environment of arsenic contamination, improve the resistance and the ability of obtaining essential mineral nutrient of ciliate desert-grass to lean soil and combined pollution, enlarge the ciliate desert-grass scope of application, promote the ciliate desert-grass growth, thereby quicken As polluted soil phytoremediation process, finally reach the long-term goal of recovering productivity of land and regional view harmony, the ecological balance and sustainable development.
For achieving the above object, technical solution of the present invention provides a kind of method that improves the As polluted soil remediation efficiency, and it inoculates arbuscular mycorrhizal fungi to pteridophyte, makes its Mycorrhizal.
The method of described As polluted soil remediation efficiency, it may further comprise the steps:
(1) gathers the Primofilicales vegetable material, obtain pteridophyta spore, cultivate the pteridophyte seedling;
(2) pteridophyte is carried out Mycorrhizal;
(3) will become the strain seedling replanting to As polluted soil.
The method of described As polluted soil remediation efficiency in its described (1) step, comprising:
A. gather the Primofilicales vegetable material in summer and autumn from the arsenic contamination area, obtain pteridophyta spore;
B. pollution-free soil is mixed obtaining culture matrix with vermiculite, after the matrix sterilization treatment, mix basal dressing, pack into basin alms bowl or seedling-cultivating tray weigh 18～20% by matrix and water, treat moisture penetration evenly after, broadcast sowing pteridophyta spore on the matrix top layer;
C. then, evenly cover the last layer fine sand, basin alms bowl or seedling-cultivating tray are sealed or cover, on preservative film, prick the hole with preservative film;
D. in culturing room that can control temperature and illumination or greenhouse, cultivate, weigh in the incubation, keep moisture 18～20%;
E. observe pteridophyta spore in the incubation and sprout and the growth of seedling situation, cultivate, can obtain standby pteridophyte seedling, height of seedling to 2～3 centimetre through 2～March;
F. the pteridophyte seedling that obtains is planted cultivation in indoor the branch, promptly take out seedling and be divided into individual plant, divide to plant and coil, make height of seedling to 5～8 centimetre to pot for growing seedlings or cave.
The method of described As polluted soil remediation efficiency, its described (2) step Mycorrhizal pteridophyte, be when pteridophyte is grown seedlings, to carry out, comprising:
A. obtain Inoculant from the mycorrhizal fungi microbial inoculum production unit of providing;
B. when pteridophyta spore grows seedlings, sneak into Inoculant when in the culture matrix of sterilization back, sneaking into base manure;
C. or when dividing cultivation to support the pteridophyte seedling, apply Inoculant again.
The method of described As polluted soil remediation efficiency, its described (3) step spreads manuer in holes into Inoculant at seedling when pteridophyte being become the strain seedling replanting go into the arsenic contamination area.
The method of described As polluted soil remediation efficiency, its described pteridophyte is ciliate desert-grass.
The method of described As polluted soil remediation efficiency, its described culture matrix were that pollution-free soil mixes by 1: 1 with vermiculite, through 10KGy irradiation sterilization or 121 ℃ of high pressure steam sterilizations 2 hours.
The method of described As polluted soil remediation efficiency, its described base manure is N, 200mg kg
-1, P, 50mg kg
-1K, 150mg kg
-1, and other trace element is an amount of.
The method of described As polluted soil remediation efficiency, its described fine sand is crossed 0.9～1.1mm sieve, cladding thickness≤1mm.
The method of described As polluted soil remediation efficiency, its described hole of on preservative film, pricking, the aperture is no more than 2mm, every square decimeter of 5～8 holes.
The method of described As polluted soil remediation efficiency, it is described cultivates in culturing room or greenhouse, 16 ℃ of temperature 25 ℃/nights of daytime, illumination 14～16 hours, intensity of illumination 550mE.m
-2.s
-1PAR, wave band 400～700nm.
The method of described As polluted soil remediation efficiency, it is described spreads manuer in holes into Inoculant at seedling, for every cave/strain applies 20～25 grams.
The method of described As polluted soil remediation efficiency, its described arbuscular mycorrhizal fungi, have the bacterial classification of positive effect of inoculation to comprise: (China Agricultural University separates Glomus mosseae, the numbering BEG167 of Europe DSMZ), Glomus intraradices (the European numbering BEG141 of DSMZ) and Glomus caledonium (Nanjing Soil Inst., Chinese Academy of Sciences, numbering 90036).
The present invention's test shows that the inoculation arbuscular mycorrhizal fungi can significantly be improved mineral nutrition (especially phosphorus nutrition) situation of ciliate desert-grass under the arsenic contamination situation, promote plant strain growth and increase absorptive amount to arsenic, thereby quicken As polluted soil phytoremediation process, finally reach the long-term goal of recovering productivity of land and regional view harmony, the ecological balance and sustainable development.
Description of drawings
Fig. 1: for forming flourishing mycorhiza structure in the ciliate desert-grass sample root of field acquisition;
Fig. 2: inoculation arbuscular mycorrhizal fungi Glomus mosseae has all promoted the ciliate desert-grass growth under the arsenic level in order to execute in difference, and last figure is the test photo; Figure below is corresponding test data figure.
Embodiment
Under the situation of arsenic and other metal combined contamination, the growth of ciliate desert-grass has very high dependence for arbuscular mycorrhiza.Inoculation is handled the survival that helps ciliate desert-grass and is significantly improved growth.
When using ciliate desert-grass reparation As polluted soil, introduce arbuscular mycorrhizal fungi and (directly apply suitable arbuscular mycorrhizal fungi Inoculant, perhaps use the Mycorrhizal seedling) assist plant to adapt to complicated pollution living environment, obtain essential mineral nutrient, thereby improve the plant individual survival rate, promote plant growing, accelerate the phytoremediation process.
The Mycorrhizal ciliate desert-grass can apply the mycorrhizal fungi Inoculant to the cave dish and realize when growing seedlings early stage, be aided with when perhaps ciliate desert-grass is planted in the field and use the mycorrhizal fungi Inoculant.
The mycorrhizal fungi Inoculant can be any type of commercialization Inoculant, and the bacterial classification that empirical evidence has positive effect of inoculation comprises Glomus mosseae, Glomus caledonium and Glomusintraradices etc.
The preparation of stock among the present invention:
One, hyperaccumulative plant ciliate desert-grass seedling obtains
(1) summer and autumn from the arsenic contamination area (as realgar mining area, Shimen County, Hunan Province) gather original ciliate desert-grass material, obtain the ciliate desert-grass spore.Preservation spore under room temperature, ventilation, drying condition.
(2) soil (pollution-free) mixes in 1: 1 ratio with vermiculite and obtains culture matrix.After matrix sterilization treatment (10KGy irradiation sterilization or 121 ℃ of high pressure steam sterilizations 2 hours are killed pathogen), mix basal dressing (N, 200mg kg
-1, P, 50mg kg
-1K, 150mg kg
-1It is an amount of also can to apply other trace element simultaneously), pack into basin alms bowl or seedling-cultivating tray weigh 20% by matrix and water.After treating moisture penetration evenly, broadcast sowing the ciliate desert-grass spore on the matrix top layer.Then, evenly cover last layer fine sand (cross the 1mm sieve, cladding thickness is no more than 1mm), basin alms bowl or seedling-cultivating tray are sealed or cover to preserve moisture, to heat, promote the ciliate desert-grass spore germination with home-use preservative film.For spore germination and the later stage growth of seedling required air of securing an adequate supply, conveniently water simultaneously, on preservative film, prick hole some (aperture is no more than 2mm, every square decimeter of 5 to 8 holes) with instruments such as tingle.
(3) in culturing room that can control temperature and illumination or greenhouse, cultivate (16 ℃ of temperature 25 ℃/nights of daytime, light application time early 6 to late 10 points, intensity of illumination 550mE.m
-2.s
-1PAR, wave band 400-700nm).Weigh for 1-2 time weekly in the incubation, keep moisture 20% (can keep the skin wet) by aperture on the preservative film.
(4) in incubation, note observing spore germination and growth of seedling situation.As seen grow green gametophyte (like green alga) general 3 weeks.Cultivate through the 2-3 month again, can obtain standby ciliate desert-grass seedling (being advisable for height of seedling 2-3 centimetre).
(5) if prepare the ciliate desert-grass direct transplanting is arrived the field, the seedling that preceding method obtains need be planted cultivation in indoor the branch in advance, promptly take out seedling and be divided into individual plant, divide and plant to pot for growing seedlings or cave dish.Culture matrix and condition of culture are same as above., can transplant to 5-8 centimetre for height of seedling.
Two, to ciliate desert-grass inoculation arbuscular mycorrhizal fungi
A) obtain Inoculant from the mycorrhizal fungi microbial inoculum production unit of providing (as the Chinese Academy of Agricultural Sciences, or external relevant unit such as European DSMZ).General Inoculant is the rhizosphere soil (or other matrix) of the mycorrhizal plants cultivated under the controlled condition, wherein contains plant root segment, fungal spore and mycelium.
B) the Mycorrhizal ciliate desert-grass can apply the mycorrhizal fungi Inoculant when growing seedlings and realize that dosage of inoculation is generally 5% of growth substrate total amount in culture matrix.Specifically applying method and period is, when being grown seedlings by the ciliate desert-grass spore, sneaks into Inoculant when sneaking into base manure in the culture matrix of sterilization back; When supporting the ciliate desert-grass seedling, a minute cultivation applies Inoculant; When the ciliate desert-grass seedling replanting is gone into the field, spread manuer in holes into Inoculant (every cave/strain applies 20 gram Inoculants) at seedling.Inoculation was for good when the Mycorrhizal effect was supported the ciliate desert-grass seedling with a minute cultivation.
C) original Inoculant can under greenhouse experiment, expand numerous, can be aforementioned matrix (essential sterilization treatment) inoculation of cultivation ciliate desert-grass seedling handle (applying 5% original mycorrhizal fungi Inoculant) cultivation host plant (as clover, corn etc.).Cultivate after 2 to 3 months results rhizosphere soil and be new mycorrhizal fungi Inoculant.
D) bacterial classification of empirical tests with positive effect of inoculation comprises that (China Agricultural University separates Glomus mosseae, the numbering BEG167 of Europe DSMZ), Glomusintraradices (the European numbering BEG141 of DSMZ) and Glomuscaledonium (Nanjing Soil Inst., Chinese Academy of Sciences, numbering 90036) etc.
Embodiment
Mycorrhizal fungi Glomus mosseae (Nicol.﹠amp; Gerd) Gerdmann ﹠amp; It is numerous that Trappe with the corn is that the host expands, and is Inoculant with the rhizosphere soil that contains the outer mycelia of host plant root segment, mycorrhizal fungal spore and root.Ciliate desert-grass (Pteris vittata L.) spore is cultivated acquisition for the seedling of transplanting through pre-.
Arsenic contamination soil picks up from Hunan Province's Chenzhou City.Basic physicochemical character: pH (1: 2.5 flooding), 8.03; The content of organic matter, 5.25%; Rapid available phosphorus (Olsen-P), 15.3mg kg
-1Total arsenic content, 74.3mg kg
-1Soil is crossed 2mm sieve, irradiation sterilization (10KGy).
The rectangular box that the employing lucite is processed into is as culture vessel.Every basin is adorned native 1.5Kg.Outside the background arsenic concentration, test is established two arsenic in addition and is applied concentration: 100mg kg
-1With 300mg kg
-1Under each arsenic concentration, ciliate desert-grass is all established inoculation and corresponding control treatment.Every basin dosage of inoculation 40g is handled in inoculation, and control treatment applies the Inoculant of equivalent through sterilization treatment, and each is handled and repeats 4 times.1 strain of every pot transplanting ciliate desert-grass seedling.
(temperature automatically controlled 25 ℃) carry out in the Ecological Environmental Research Center greenhouse in test, adopt Weighing method control moisture between culture period.
Cultivation is through 3 months, can observe under three arsenic concentrations inoculation and handle and all obviously promoted ciliate desert-grass growth (referring to accompanying drawing 2).
Claims (13)
1. a method that improves As polluted soil phytoremediation efficient is characterized in that, to pteridophyte inoculation arbuscular mycorrhizal fungi, makes its Mycorrhizal.
2. the method for As polluted soil remediation efficiency as claimed in claim 1 is characterized in that, may further comprise the steps:
(1) gathers the Primofilicales vegetable material, obtain pteridophyta spore, cultivate the pteridophyte seedling;
(2) pteridophyte is carried out Mycorrhizal;
(3) will become the strain seedling replanting to As polluted soil.
3. the method for As polluted soil phytoremediation efficient as claimed in claim 2 is characterized in that, in described (1) step, comprising:
A. gather the Primofilicales vegetable material in summer and autumn from the arsenic contamination area, obtain pteridophyta spore;
B. pollution-free soil is mixed obtaining culture matrix with vermiculite, after the matrix sterilization treatment, mix basal dressing, pack into basin alms bowl or seedling-cultivating tray weigh 18～20% by matrix and water, treat moisture penetration evenly after, broadcast sowing pteridophyta spore on the matrix top layer;
C. then, evenly cover the last layer fine sand, basin alms bowl or seedling-cultivating tray are sealed or cover, on preservative film, prick the hole with preservative film;
D. in culturing room that can control temperature and illumination or greenhouse, cultivate, weigh in the incubation, keep moisture 18～20%;
E. observe pteridophyta spore in the incubation and sprout and the growth of seedling situation, cultivate, can obtain standby pteridophyte seedling, height of seedling to 2～3 centimetre through 2～March;
F. the pteridophyte seedling that obtains is planted cultivation in indoor the branch, promptly take out seedling and be divided into individual plant, divide to plant and coil, make height of seedling to 5～8 centimetre to pot for growing seedlings or cave.
4. the method for As polluted soil phytoremediation efficient as claimed in claim 2 is characterized in that, described (2) goes on foot the Mycorrhizal pteridophyte, is to carry out when pteridophyte is grown seedlings, and comprising:
A. obtain Inoculant from the mycorrhizal fungi microbial inoculum production unit of providing;
B. when pteridophyta spore grows seedlings, sneak into Inoculant when in the culture matrix of sterilization back, sneaking into base manure;
C. or when dividing cultivation to support the pteridophyte seedling, apply Inoculant again.
5. the method for As polluted soil phytoremediation efficient as claimed in claim 2 is characterized in that, described (3) step spreads manuer in holes into Inoculant at seedling when pteridophyte being become the strain seedling replanting go into the arsenic contamination area.
6. the method for As polluted soil phytoremediation efficient as claimed in claim 3 is characterized in that, described pteridophyte is ciliate desert-grass.
7. the method for As polluted soil phytoremediation efficient as claimed in claim 3 is characterized in that, described culture matrix is that pollution-free soil mixes by 1: 1 with vermiculite, through 10KGy irradiation sterilization or 121 ℃ of high pressure steam sterilizations 2 hours.
8. the method for As polluted soil phytoremediation efficient as claimed in claim 3 is characterized in that, described base manure is N, 200mg kg
-1, P, 50mg kg
-1K, 150mg kg
-1, and other trace element is an amount of.
9. the method for As polluted soil phytoremediation efficient as claimed in claim 3 is characterized in that, described fine sand is crossed 0.9～1.1mm sieve, cladding thickness≤1mm.
10. the method for As polluted soil remediation efficiency as claimed in claim 3 is characterized in that, the described hole of on preservative film, pricking, and the aperture is no more than 2mm, every square decimeter of 5～8 holes.
11. the method for As polluted soil phytoremediation efficient as claimed in claim 3 is characterized in that, describedly cultivates 16 ℃ of temperature 25 ℃/nights of daytime, illumination 14～16 hours, intensity of illumination 550mE.m in culturing room or greenhouse
-2.s
-1PAR, wave band 400～700nm.
12. the method for As polluted soil phytoremediation efficient as claimed in claim 5 is characterized in that, describedly spreads manuer in holes into Inoculant at seedling, for every cave/strain applies 20～25 grams.
13. the method for As polluted soil phytoremediation efficient as claimed in claim 1, it is characterized in that, described arbuscular mycorrhizal fungi, have the bacterial classification of positive effect of inoculation to comprise: (China Agricultural University separates Glomus mosseae, the numbering BEG167 of Europe DSMZ), Glomusintraradices (the European numbering BEG141 of DSMZ) and Glomus caledonium (Nanjing Soil Inst., Chinese Academy of Sciences, numbering 90036).
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| CN 200310123407 CN1633834A (en) | 2003-12-26 | 2003-12-26 | Method for promoting plant restoration efficiency of arsenic polluted soil |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100384760C (en) * | 2005-09-22 | 2008-04-30 | 中国科学院地理科学与资源研究所 | Arsenic polluted water body repairing method and apparatus |
| CN102039305A (en) * | 2009-10-23 | 2011-05-04 | 吴江市土壤肥料技术指导站 | Method for improving repair efficiency of plant on low As and Hg combined contamination soil |
| CN101735997B (en) * | 2009-12-10 | 2011-12-14 | 中国科学院地理科学与资源研究所 | Microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof |
| CN104429845A (en) * | 2014-09-25 | 2015-03-25 | 阜南县永兴工艺品有限公司 | Efficient planting method for chaste trees |
| CN105409565A (en) * | 2015-12-15 | 2016-03-23 | 中国科学院地理科学与资源研究所 | Industrialized seedling method for super-enriched plant herb of Chinese brake |
| CN105945042A (en) * | 2016-06-02 | 2016-09-21 | 龚松贵 | Method for removing soil arsenic pollution by interplanting pteris vittata |
| CN105985783A (en) * | 2016-02-24 | 2016-10-05 | 凤阳徽亨商贸有限公司 | Soil heavy metal restoration agent capable of enhancing organic carbon level |
| CN106613160A (en) * | 2016-11-23 | 2017-05-10 | 云南云投生态环境科技股份有限公司 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
| CN112974510A (en) * | 2021-03-19 | 2021-06-18 | 交大环境保护研究院(江苏)有限公司 | Application of ciliate desert-grass in repairing heavy metal arsenic pollution of soil in mine |
| CN113068568A (en) * | 2021-03-16 | 2021-07-06 | 西南林业大学 | Soil for quickly cultivating ciliate desert-grass, cultivation method and application |
-
2003
- 2003-12-26 CN CN 200310123407 patent/CN1633834A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100384760C (en) * | 2005-09-22 | 2008-04-30 | 中国科学院地理科学与资源研究所 | Arsenic polluted water body repairing method and apparatus |
| CN102039305A (en) * | 2009-10-23 | 2011-05-04 | 吴江市土壤肥料技术指导站 | Method for improving repair efficiency of plant on low As and Hg combined contamination soil |
| CN101735997B (en) * | 2009-12-10 | 2011-12-14 | 中国科学院地理科学与资源研究所 | Microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof |
| CN104429845A (en) * | 2014-09-25 | 2015-03-25 | 阜南县永兴工艺品有限公司 | Efficient planting method for chaste trees |
| CN105409565A (en) * | 2015-12-15 | 2016-03-23 | 中国科学院地理科学与资源研究所 | Industrialized seedling method for super-enriched plant herb of Chinese brake |
| CN105985783A (en) * | 2016-02-24 | 2016-10-05 | 凤阳徽亨商贸有限公司 | Soil heavy metal restoration agent capable of enhancing organic carbon level |
| CN105945042A (en) * | 2016-06-02 | 2016-09-21 | 龚松贵 | Method for removing soil arsenic pollution by interplanting pteris vittata |
| CN106613160A (en) * | 2016-11-23 | 2017-05-10 | 云南云投生态环境科技股份有限公司 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
| CN113068568A (en) * | 2021-03-16 | 2021-07-06 | 西南林业大学 | Soil for quickly cultivating ciliate desert-grass, cultivation method and application |
| CN112974510A (en) * | 2021-03-19 | 2021-06-18 | 交大环境保护研究院(江苏)有限公司 | Application of ciliate desert-grass in repairing heavy metal arsenic pollution of soil in mine |
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