CN106613160A - Spore breeding method for arsenic hyperaccumulator Pteris cretica - Google Patents

Spore breeding method for arsenic hyperaccumulator Pteris cretica Download PDF

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Publication number
CN106613160A
CN106613160A CN201611040917.XA CN201611040917A CN106613160A CN 106613160 A CN106613160 A CN 106613160A CN 201611040917 A CN201611040917 A CN 201611040917A CN 106613160 A CN106613160 A CN 106613160A
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China
Prior art keywords
seedling
spore
mating system
system described
arsenic
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CN201611040917.XA
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Chinese (zh)
Inventor
童亚丽
鲁明秀
薛必猛
宣艳辉
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Yunnan Cloud Cast Ecological Environment Polytron Technologies Inc
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Yunnan Cloud Cast Ecological Environment Polytron Technologies Inc
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Priority to CN201611040917.XA priority Critical patent/CN106613160A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a spore breeding method for arsenic hyperaccumulator Pteris cretica. The spore breeding method is implemented and completed by parts, such as a hybrid seedling matrix, a seedling pot, mature Pteris cretica spores and special management and protection and observation. The spore breeding method is capable of, according to the problems that the spore volume of the Pteris cretica spore seedling is small, the seeding rate is hard to be controlled, and the efficiency of forming sporophyte by gametophyte in the natural conditions is low, designing the spore breeding technology, solving the problem that the qualified seedling percentage of the Pteris cretica spore breeding in the natural conditions is low, and designing the complete breeding management technology in allusion to the special requirements of water, illumination and temperature in the breeding period of the Pteris cretica spore, and can be used as a method for breeding the arsenic hyperaccumulator Pteris cretica spores.

Description

A kind of sporogenesis method of arsenic super enriching plant cretan brake
Technical field
The present invention relates to heavy metal super-enriched pteridophyta spore mating system, particularly Pteridaceae Pteris is super The spore mating system of enriching plant cretan brake (Pteris cretica var.nervosa), be one kind meet arsenic surpass The sprouting of enriching plant cretan brake spore, the formation of gametophytic development and new sporinite, sporinite Seedling Stage are managed and protected Method, be specifically related to a kind of sporogenesis method of arsenic super enriching plant cretan brake.
Background technology
Water body and heavy metal pollution of soil problem are one of puzzlement global environment problems, and in recent years, some can be on ground Top is enriched with a large number the special plant of pollutant --- and super enriching plant (hyperaccumulator) has become academia and has ground The focus studied carefully.Metal and metalloid pollution --- the phytoremediation in soil and water body environment is removed using super enriching plant (phytoremediation) technology obtains extensive concern with its potential efficiently, cheap and its environment friendly.By planting Plant the arsenic pollution that this plant of harvesting can be effectively removed in environment.As super enriching plant is extensively applied, wild resource gathers Reduce, it breeds speed naturally cannot meet the demand to super enriching plant, so as to artificial breeding in super enriching plant The exploration of method is very active.
The Pteris plant of arsenic can be in a large number enriched with --- Herba pteridis vittatae (pteris vittata L.) is repaiied in arsenic pollution Widely apply in journey of returning to work.Newfound another kind has the Pteris plant of accumulation ability to arsenic just after Herba pteridis vittatae It is cretan brake, its popularization and application area just expands year by year, and the market demand will be increasing.Pteridophyta mainly passes through spore Son is bred, its under field conditions (factors) growth cycle it is longer, planting percent is low, it is difficult to meet actual application demand.Although group It is a kind of feasible cretan brake quick breeding technology to knit culture, but its relatively costly and complex operation, how quick Jing Ji and easily cultivate cretan brake seedling on a large scale reducing rehabilitation cost, remain the popularization of arsenic pollution recovery project The urgent need to solve the problem using in.
The content of the invention
Object of the present invention is to provide one kind can be used for arsenic super enriching plant cretan brake carries out scale people The method that work is bred.The method can ensure the sprouting of cretan brake spore, prothallus development, the formation of new sporinite and Condition needed for new sporinite seedling growth, and it is easy to scale breeding to operate.Bred to educating according to cretan brake spore The demand of the acid-base value of Seedling substrate, breathability, water-retaining property and special dietary material, devises the propagation method.In view of spore Small volume, it is difficult to the difficulty of sowing, devises the method using spore suspension is sowed in liquid, to control sowing Amount and the sowing uniformity.To solve the problems, such as that it is low that cretan brake spore breeds middle planting percent, different thicknesss of sowing are devised, Variety classes, the nutritional solution of concentration and hormone induction, improve germination rate, the original foliage rate of increase and the spore transformation rate of spore, Reach the effect for improving planting percent.The method is simple to operate, and the super enriching plant seedling bred is neatly healthy and strong, can be soil remediation Large batch of super enriching plant seedling is provided.
A kind of arsenic super enriching plant cretan brake spore mating system that the present invention is provided, specifically includes following step Suddenly:
The first step, will meet the seedling medium formula of arsenic super enriching plant cretan brake seedling from spore requirement, pass through Proportioning is mixed, and forms cretan brake seedling medium;
Second step, the seedling medium obtained by the first step is added and is adapted in the seedling-growing container of cretan brake seedling from spore, Light pressure, the sedimentation for making seedling medium meets cretan brake seedling from spore requirement;
3rd step, will be seeded into the seedling-growing container table equipped with seedling medium after the collection of cretan brake spore, weighing, immersion Face;
4th step, the seedling-growing container sowed is placed in seedling raising greenhouse, pours permeable;
5th step, will complete the seedling-growing container cover film of FOUR EASY STEPS, sunshade net and ensure the temperature of spore germination, wet Degree, illumination condition;
6th step, routine observation carries out nebulizing spray, keeps the moisture condition of spore germination;
7th step, spore germination forms original foliage, and trophophase sprays nutritional solution 1, promotes phyllogen bulk-growth;
8th step, in the phyllome period of maturation, sprays nutritional solution 2, promotes phyllogen body maturation, forms ripe gamogenesis cell, And then form new sporinite;
9th step, sporinite trophophase, periodically sprinkle nutritional solution 3 so as to healthy and strong seedling;
Tenth step, seedling regular thinning, transplanting after growing up to.
Arsenic super enriching plant cretan brake spore mating system of the present invention, compared with traditional method for culturing seedlings, more Meet the demand of the sprouting of cretan brake original foliage and sporinite primary growth, and it is easy to operate, it is adapted to scale breeding behaviour Make.The seedling medium prepared of the present invention containing the basic nutrient condition needed for plant growing, acid-base value control pH5-7 it Between, seedling medium porosity is between 45%-55%.In sowing time, thickness of sowing is controlled, be advisable in 30-40mg/ basins, be shortening Breed the phase, improve planting percent, soaked 24 hours with clear water before spore sowing, in spore phase sprinkling Different Nutrition liquid is bred.Entirely During nursery, more than 80%, temperature carries out 75% density sunshade net shading light culture to humid control between 15-30 DEG C.
The present invention compared with prior art, its remarkable advantage:(1) cretan brake spore germination and the prophyll bodily form are shortened Cheng Qi, by using liquid soak 24 hours after sow, shorten original foliage sprout time;(2) cretan brake prophyll is improved Body germination rate, by spraying vegetative multiplication liquid using original foliage trophophase, promotes growth and the propagation of original foliage, reaches raising former Leaf body germination rate;(3) formation rate of the new sporinite of cretan brake is improved, by spraying nutritional solution 3 in the original foliage period of maturation, Effectively improve the quantity of the formation of sporinite;(4) the application present invention cultivates cretan brake seedling, with easy to operate, educates Seedling stage is short, and planting percent is high, and the characteristics of seedling is healthy and strong, the recovery project for heavy-metal contaminated soil provides a kind of super enriching plant Mating system, with preferable application prospect and economic benefit.
Specific embodiment
Below by embodiment, the present invention is described further
Embodiment 1
Prepared by arsenic super enriching plant cretan brake seedling medium, using matching somebody with somebody for turfy soil 60% and perlite 40% Than mixing is mixed thoroughly, in being dispensed into 10*10 seedling-growing containers, then carries out substrate sterilization with 800 times of liquid of carbendazim;
Embodiment 2
Arsenic super enriching plant cretan brake spore powder is sowed, and the annual 7-9 months, the same day is to cretan brake maternal plant garden In, the healthy and strong ripe sporophyll of collection collects spore powder, while spore powder has been weighed into 30-40mg/ parts, and is used clear water Immersion, during the substrate basin got ready was seeded in after 24 hours.
Embodiment 3
Arsenic super enriching plant cretan brake seedling stage is managed and protected, and the seedling-growing container sowed is poured into permeable, is positioned over nursery big Canopy, covers plastic sheeting, and 75% sunshade net culture, periodically takes off film and breathes freely, and periodically water spray keeps humidity.Nurturing period canopy interior temperature Between 15-30 DEG C, humidity more than 80% in regular seedling-growing container keeps relatively dark optical culture for degree control.
Sowing 10-15d or so, it is found that obvious spore germination forms the filamentous of green, 30-35d or so and forms a large amount of Heart-shaped original foliage, while spray pH6,1/2MS+20g/l sucrose+1g/lKH2PO4+ 0.2mg/lNAA breeds nutritional solution 50ml/ basins;After 60-70d, phyllome developmental capacity is good, and phyllome is paved with basin, and archegonium, spermatangium are formed, original foliage substantially into It is ripe, while spraying 50mg/lGA350ml/ basins;80-90d or so, new sporinite is formed, and gradually grows up to whole plant, afterwards per half 1000 times of foliage fertilizer 50ml/ basins are sprayed once within individual month, until into transplantation of seedlings;120d or so, seedling length is proceeded by 2-5cm Thinning is transplanted to hole tray, 150d or so, and seedling length to 15-20cm can carry out changing bag culture.
Above-described embodiment technology design only to illustrate the invention and feature, its object is to provide those skilled in the art Understand and present disclosure and implement according to this, not specific embodiment is enumerated, and can not limit protection of the invention with this Scope.All technology according to the present invention schemes are modified or equivalent, without deviating from the objective of technical solution of the present invention And scope, it all should cover in the middle of scope of the presently claimed invention.

Claims (8)

1. a kind of mating system of arsenic super enriching plant cretan brake spore, it is characterised in that comprise the following steps:
The first step, will meet the seedling medium formula of arsenic super enriching plant cretan brake seedling from spore requirement, by proportioning Mixed, formed cretan brake seedling medium;
Second step, the seedling medium obtained by the first step is added and is adapted in the seedling-growing container of cretan brake seedling from spore, light pressure, The sedimentation for making seedling medium meets cretan brake seedling from spore requirement, and is irrigated with bactericidal liquid, sterilizes standby;
3rd step, will be seeded into the seedling-growing container equipped with seedling medium after the collection of cretan brake spore, weighing, soak immersion Surface;
4th step, the seedling-growing container sowed is placed in seedling raising greenhouse, pours permeable;
5th step, will complete the seedling-growing container cover film of FOUR EASY STEPS, sunshade net and ensure temperature, humidity, the light of spore germination According to condition;
6th step, routine observation carries out nebulizing spray, keeps the moisture condition of spore germination;
7th step, spore germination forms original foliage, and trophophase sprays nutritional solution 1, promotes prophyll bulk-growth;
8th step, in the original foliage period of maturation, sprays nutritional solution 2, promotes prophyll body maturation, forms ripe gamogenesis cell, and then Form new sporinite;
9th step, sporinite trophophase, periodically sprinkle nutritional solution 3 so as to healthy and strong seedling;
Tenth step, seedling regular thinning, transplanting after growing up to.
2., according to the mating system described in claim 1, the substrate composition in the first step is:
The proportioning of turfy soil 60% and perlite 40%, acid-base value is controlled in pH5-7, and seedling medium porosity is in 45%-55%.
3., according to the mating system described in claim 1, the disinfectant solution in second step is:800 times of carbendazim liquid.
4., according to the mating system described in claim 1, the soak in the 3rd step is:Clear water;Suitable thickness of sowing is: 30-40mg/ basins.
5., according to the mating system described in claim 1, it is bred condition of culture and is:Temperature:15-30 DEG C, moisture:Water in basin Point humid control is more than 80%;Illumination:Before transplanting, 75% sunshade net half-light culture is carried out.
6., according to the mating system described in claim 1, the nutritional solution 1 in the 7th step is:PH6,1/2MS+20g/l sucrose+ 1g/lKH2PO4+ 0.2mg/lNAA, consumption is:50ml/ basins.
7., according to the mating system described in claim 1, the nutritional solution 2 in the 8th step is:
50mg/lGA3Consumption is:50ml/ basins.
8., according to the mating system described in claim 1, the nutritional solution 6 in the 8th step is:1000 times of N-P-K20-20-20's Foliage fertilizer, consumption is:50ml/ basins.
CN201611040917.XA 2016-11-23 2016-11-23 Spore breeding method for arsenic hyperaccumulator Pteris cretica Pending CN106613160A (en)

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CN108575714A (en) * 2018-05-17 2018-09-28 湖南省农业环境生态研究所 A kind of ciliate desert-grass spore germination and intensive seedling production method
CN111096173A (en) * 2020-01-06 2020-05-05 浙江省亚热带作物研究所 Method for promoting pen container tree development through adjusting environmental factors

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
CN108575714A (en) * 2018-05-17 2018-09-28 湖南省农业环境生态研究所 A kind of ciliate desert-grass spore germination and intensive seedling production method
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CN111096173A (en) * 2020-01-06 2020-05-05 浙江省亚热带作物研究所 Method for promoting pen container tree development through adjusting environmental factors
CN111096173B (en) * 2020-01-06 2021-09-14 浙江省亚热带作物研究所 Method for promoting pen container tree development through adjusting environmental factors

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Application publication date: 20170510