CN106613160A - Spore breeding method for arsenic hyperaccumulator Pteris cretica - Google Patents
Spore breeding method for arsenic hyperaccumulator Pteris cretica Download PDFInfo
- Publication number
- CN106613160A CN106613160A CN201611040917.XA CN201611040917A CN106613160A CN 106613160 A CN106613160 A CN 106613160A CN 201611040917 A CN201611040917 A CN 201611040917A CN 106613160 A CN106613160 A CN 106613160A
- Authority
- CN
- China
- Prior art keywords
- seedling
- spore
- mating system
- system described
- arsenic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a spore breeding method for arsenic hyperaccumulator Pteris cretica. The spore breeding method is implemented and completed by parts, such as a hybrid seedling matrix, a seedling pot, mature Pteris cretica spores and special management and protection and observation. The spore breeding method is capable of, according to the problems that the spore volume of the Pteris cretica spore seedling is small, the seeding rate is hard to be controlled, and the efficiency of forming sporophyte by gametophyte in the natural conditions is low, designing the spore breeding technology, solving the problem that the qualified seedling percentage of the Pteris cretica spore breeding in the natural conditions is low, and designing the complete breeding management technology in allusion to the special requirements of water, illumination and temperature in the breeding period of the Pteris cretica spore, and can be used as a method for breeding the arsenic hyperaccumulator Pteris cretica spores.
Description
Technical field
The present invention relates to heavy metal super-enriched pteridophyta spore mating system, particularly Pteridaceae Pteris is super
The spore mating system of enriching plant cretan brake (Pteris cretica var.nervosa), be one kind meet arsenic surpass
The sprouting of enriching plant cretan brake spore, the formation of gametophytic development and new sporinite, sporinite Seedling Stage are managed and protected
Method, be specifically related to a kind of sporogenesis method of arsenic super enriching plant cretan brake.
Background technology
Water body and heavy metal pollution of soil problem are one of puzzlement global environment problems, and in recent years, some can be on ground
Top is enriched with a large number the special plant of pollutant --- and super enriching plant (hyperaccumulator) has become academia and has ground
The focus studied carefully.Metal and metalloid pollution --- the phytoremediation in soil and water body environment is removed using super enriching plant
(phytoremediation) technology obtains extensive concern with its potential efficiently, cheap and its environment friendly.By planting
Plant the arsenic pollution that this plant of harvesting can be effectively removed in environment.As super enriching plant is extensively applied, wild resource gathers
Reduce, it breeds speed naturally cannot meet the demand to super enriching plant, so as to artificial breeding in super enriching plant
The exploration of method is very active.
The Pteris plant of arsenic can be in a large number enriched with --- Herba pteridis vittatae (pteris vittata L.) is repaiied in arsenic pollution
Widely apply in journey of returning to work.Newfound another kind has the Pteris plant of accumulation ability to arsenic just after Herba pteridis vittatae
It is cretan brake, its popularization and application area just expands year by year, and the market demand will be increasing.Pteridophyta mainly passes through spore
Son is bred, its under field conditions (factors) growth cycle it is longer, planting percent is low, it is difficult to meet actual application demand.Although group
It is a kind of feasible cretan brake quick breeding technology to knit culture, but its relatively costly and complex operation, how quick Jing
Ji and easily cultivate cretan brake seedling on a large scale reducing rehabilitation cost, remain the popularization of arsenic pollution recovery project
The urgent need to solve the problem using in.
The content of the invention
Object of the present invention is to provide one kind can be used for arsenic super enriching plant cretan brake carries out scale people
The method that work is bred.The method can ensure the sprouting of cretan brake spore, prothallus development, the formation of new sporinite and
Condition needed for new sporinite seedling growth, and it is easy to scale breeding to operate.Bred to educating according to cretan brake spore
The demand of the acid-base value of Seedling substrate, breathability, water-retaining property and special dietary material, devises the propagation method.In view of spore
Small volume, it is difficult to the difficulty of sowing, devises the method using spore suspension is sowed in liquid, to control sowing
Amount and the sowing uniformity.To solve the problems, such as that it is low that cretan brake spore breeds middle planting percent, different thicknesss of sowing are devised,
Variety classes, the nutritional solution of concentration and hormone induction, improve germination rate, the original foliage rate of increase and the spore transformation rate of spore,
Reach the effect for improving planting percent.The method is simple to operate, and the super enriching plant seedling bred is neatly healthy and strong, can be soil remediation
Large batch of super enriching plant seedling is provided.
A kind of arsenic super enriching plant cretan brake spore mating system that the present invention is provided, specifically includes following step
Suddenly:
The first step, will meet the seedling medium formula of arsenic super enriching plant cretan brake seedling from spore requirement, pass through
Proportioning is mixed, and forms cretan brake seedling medium;
Second step, the seedling medium obtained by the first step is added and is adapted in the seedling-growing container of cretan brake seedling from spore,
Light pressure, the sedimentation for making seedling medium meets cretan brake seedling from spore requirement;
3rd step, will be seeded into the seedling-growing container table equipped with seedling medium after the collection of cretan brake spore, weighing, immersion
Face;
4th step, the seedling-growing container sowed is placed in seedling raising greenhouse, pours permeable;
5th step, will complete the seedling-growing container cover film of FOUR EASY STEPS, sunshade net and ensure the temperature of spore germination, wet
Degree, illumination condition;
6th step, routine observation carries out nebulizing spray, keeps the moisture condition of spore germination;
7th step, spore germination forms original foliage, and trophophase sprays nutritional solution 1, promotes phyllogen bulk-growth;
8th step, in the phyllome period of maturation, sprays nutritional solution 2, promotes phyllogen body maturation, forms ripe gamogenesis cell,
And then form new sporinite;
9th step, sporinite trophophase, periodically sprinkle nutritional solution 3 so as to healthy and strong seedling;
Tenth step, seedling regular thinning, transplanting after growing up to.
Arsenic super enriching plant cretan brake spore mating system of the present invention, compared with traditional method for culturing seedlings, more
Meet the demand of the sprouting of cretan brake original foliage and sporinite primary growth, and it is easy to operate, it is adapted to scale breeding behaviour
Make.The seedling medium prepared of the present invention containing the basic nutrient condition needed for plant growing, acid-base value control pH5-7 it
Between, seedling medium porosity is between 45%-55%.In sowing time, thickness of sowing is controlled, be advisable in 30-40mg/ basins, be shortening
Breed the phase, improve planting percent, soaked 24 hours with clear water before spore sowing, in spore phase sprinkling Different Nutrition liquid is bred.Entirely
During nursery, more than 80%, temperature carries out 75% density sunshade net shading light culture to humid control between 15-30 DEG C.
The present invention compared with prior art, its remarkable advantage:(1) cretan brake spore germination and the prophyll bodily form are shortened
Cheng Qi, by using liquid soak 24 hours after sow, shorten original foliage sprout time;(2) cretan brake prophyll is improved
Body germination rate, by spraying vegetative multiplication liquid using original foliage trophophase, promotes growth and the propagation of original foliage, reaches raising former
Leaf body germination rate;(3) formation rate of the new sporinite of cretan brake is improved, by spraying nutritional solution 3 in the original foliage period of maturation,
Effectively improve the quantity of the formation of sporinite;(4) the application present invention cultivates cretan brake seedling, with easy to operate, educates
Seedling stage is short, and planting percent is high, and the characteristics of seedling is healthy and strong, the recovery project for heavy-metal contaminated soil provides a kind of super enriching plant
Mating system, with preferable application prospect and economic benefit.
Specific embodiment
Below by embodiment, the present invention is described further
Embodiment 1
Prepared by arsenic super enriching plant cretan brake seedling medium, using matching somebody with somebody for turfy soil 60% and perlite 40%
Than mixing is mixed thoroughly, in being dispensed into 10*10 seedling-growing containers, then carries out substrate sterilization with 800 times of liquid of carbendazim;
Embodiment 2
Arsenic super enriching plant cretan brake spore powder is sowed, and the annual 7-9 months, the same day is to cretan brake maternal plant garden
In, the healthy and strong ripe sporophyll of collection collects spore powder, while spore powder has been weighed into 30-40mg/ parts, and is used clear water
Immersion, during the substrate basin got ready was seeded in after 24 hours.
Embodiment 3
Arsenic super enriching plant cretan brake seedling stage is managed and protected, and the seedling-growing container sowed is poured into permeable, is positioned over nursery big
Canopy, covers plastic sheeting, and 75% sunshade net culture, periodically takes off film and breathes freely, and periodically water spray keeps humidity.Nurturing period canopy interior temperature
Between 15-30 DEG C, humidity more than 80% in regular seedling-growing container keeps relatively dark optical culture for degree control.
Sowing 10-15d or so, it is found that obvious spore germination forms the filamentous of green, 30-35d or so and forms a large amount of
Heart-shaped original foliage, while spray pH6,1/2MS+20g/l sucrose+1g/lKH2PO4+ 0.2mg/lNAA breeds nutritional solution
50ml/ basins;After 60-70d, phyllome developmental capacity is good, and phyllome is paved with basin, and archegonium, spermatangium are formed, original foliage substantially into
It is ripe, while spraying 50mg/lGA350ml/ basins;80-90d or so, new sporinite is formed, and gradually grows up to whole plant, afterwards per half
1000 times of foliage fertilizer 50ml/ basins are sprayed once within individual month, until into transplantation of seedlings;120d or so, seedling length is proceeded by 2-5cm
Thinning is transplanted to hole tray, 150d or so, and seedling length to 15-20cm can carry out changing bag culture.
Above-described embodiment technology design only to illustrate the invention and feature, its object is to provide those skilled in the art
Understand and present disclosure and implement according to this, not specific embodiment is enumerated, and can not limit protection of the invention with this
Scope.All technology according to the present invention schemes are modified or equivalent, without deviating from the objective of technical solution of the present invention
And scope, it all should cover in the middle of scope of the presently claimed invention.
Claims (8)
1. a kind of mating system of arsenic super enriching plant cretan brake spore, it is characterised in that comprise the following steps:
The first step, will meet the seedling medium formula of arsenic super enriching plant cretan brake seedling from spore requirement, by proportioning
Mixed, formed cretan brake seedling medium;
Second step, the seedling medium obtained by the first step is added and is adapted in the seedling-growing container of cretan brake seedling from spore, light pressure,
The sedimentation for making seedling medium meets cretan brake seedling from spore requirement, and is irrigated with bactericidal liquid, sterilizes standby;
3rd step, will be seeded into the seedling-growing container equipped with seedling medium after the collection of cretan brake spore, weighing, soak immersion
Surface;
4th step, the seedling-growing container sowed is placed in seedling raising greenhouse, pours permeable;
5th step, will complete the seedling-growing container cover film of FOUR EASY STEPS, sunshade net and ensure temperature, humidity, the light of spore germination
According to condition;
6th step, routine observation carries out nebulizing spray, keeps the moisture condition of spore germination;
7th step, spore germination forms original foliage, and trophophase sprays nutritional solution 1, promotes prophyll bulk-growth;
8th step, in the original foliage period of maturation, sprays nutritional solution 2, promotes prophyll body maturation, forms ripe gamogenesis cell, and then
Form new sporinite;
9th step, sporinite trophophase, periodically sprinkle nutritional solution 3 so as to healthy and strong seedling;
Tenth step, seedling regular thinning, transplanting after growing up to.
2., according to the mating system described in claim 1, the substrate composition in the first step is:
The proportioning of turfy soil 60% and perlite 40%, acid-base value is controlled in pH5-7, and seedling medium porosity is in 45%-55%.
3., according to the mating system described in claim 1, the disinfectant solution in second step is:800 times of carbendazim liquid.
4., according to the mating system described in claim 1, the soak in the 3rd step is:Clear water;Suitable thickness of sowing is:
30-40mg/ basins.
5., according to the mating system described in claim 1, it is bred condition of culture and is:Temperature:15-30 DEG C, moisture:Water in basin
Point humid control is more than 80%;Illumination:Before transplanting, 75% sunshade net half-light culture is carried out.
6., according to the mating system described in claim 1, the nutritional solution 1 in the 7th step is:PH6,1/2MS+20g/l sucrose+
1g/lKH2PO4+ 0.2mg/lNAA, consumption is:50ml/ basins.
7., according to the mating system described in claim 1, the nutritional solution 2 in the 8th step is:
50mg/lGA3Consumption is:50ml/ basins.
8., according to the mating system described in claim 1, the nutritional solution 6 in the 8th step is:1000 times of N-P-K20-20-20's
Foliage fertilizer, consumption is:50ml/ basins.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611040917.XA CN106613160A (en) | 2016-11-23 | 2016-11-23 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611040917.XA CN106613160A (en) | 2016-11-23 | 2016-11-23 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106613160A true CN106613160A (en) | 2017-05-10 |
Family
ID=58811463
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201611040917.XA Pending CN106613160A (en) | 2016-11-23 | 2016-11-23 | Spore breeding method for arsenic hyperaccumulator Pteris cretica |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106613160A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108575714A (en) * | 2018-05-17 | 2018-09-28 | 湖南省农业环境生态研究所 | A kind of ciliate desert-grass spore germination and intensive seedling production method |
CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1502423A (en) * | 2002-11-27 | 2004-06-09 | 中国科学院地理科学与资源研究所 | Method for repairing arsenium contaminated environment |
CN1600707A (en) * | 2004-10-20 | 2005-03-30 | 中山大学 | Method for governing arsenic contamination in soil and water body |
CN1633834A (en) * | 2003-12-26 | 2005-07-06 | 中国科学院生态环境研究中心 | Method for promoting plant restoration efficiency of arsenic polluted soil |
CN101297635A (en) * | 2008-06-30 | 2008-11-05 | 中国科学院华南植物园 | Method for breeding spore of Dryopteris varia |
CN101773032A (en) * | 2010-01-08 | 2010-07-14 | 山东理工大学 | Quick propagation seedling establishment method for bracken spore matrix |
CN103081745A (en) * | 2013-02-01 | 2013-05-08 | 中国科学院植物研究所 | Pteridophyte seedling raising device |
CN104663200A (en) * | 2015-02-03 | 2015-06-03 | 深圳市铁汉生态环境股份有限公司 | Mass pteridophyta reproduction method |
CN105265064A (en) * | 2015-11-17 | 2016-01-27 | 四川农业大学 | Liquid culture seedling growing method for fern protonemata |
CN105409565A (en) * | 2015-12-15 | 2016-03-23 | 中国科学院地理科学与资源研究所 | Industrialized seedling method for super-enriched plant herb of Chinese brake |
CN105475139A (en) * | 2015-12-24 | 2016-04-13 | 深圳市铁汉生态环境股份有限公司 | Tissue culture propagation method of Adiantum hispidulum |
-
2016
- 2016-11-23 CN CN201611040917.XA patent/CN106613160A/en active Pending
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1502423A (en) * | 2002-11-27 | 2004-06-09 | 中国科学院地理科学与资源研究所 | Method for repairing arsenium contaminated environment |
CN1633834A (en) * | 2003-12-26 | 2005-07-06 | 中国科学院生态环境研究中心 | Method for promoting plant restoration efficiency of arsenic polluted soil |
CN1600707A (en) * | 2004-10-20 | 2005-03-30 | 中山大学 | Method for governing arsenic contamination in soil and water body |
CN101297635A (en) * | 2008-06-30 | 2008-11-05 | 中国科学院华南植物园 | Method for breeding spore of Dryopteris varia |
CN101773032A (en) * | 2010-01-08 | 2010-07-14 | 山东理工大学 | Quick propagation seedling establishment method for bracken spore matrix |
CN103081745A (en) * | 2013-02-01 | 2013-05-08 | 中国科学院植物研究所 | Pteridophyte seedling raising device |
CN104663200A (en) * | 2015-02-03 | 2015-06-03 | 深圳市铁汉生态环境股份有限公司 | Mass pteridophyta reproduction method |
CN105265064A (en) * | 2015-11-17 | 2016-01-27 | 四川农业大学 | Liquid culture seedling growing method for fern protonemata |
CN105409565A (en) * | 2015-12-15 | 2016-03-23 | 中国科学院地理科学与资源研究所 | Industrialized seedling method for super-enriched plant herb of Chinese brake |
CN105475139A (en) * | 2015-12-24 | 2016-04-13 | 深圳市铁汉生态环境股份有限公司 | Tissue culture propagation method of Adiantum hispidulum |
Non-Patent Citations (3)
Title |
---|
岳思君等: "蕨孢子培养", 《宁夏农学院学报》 * |
王德群: "《药用植物生态学》", 30 April 2006, 中国中医药出版社 * |
郭成金: "《实用覃菌生物学》", 30 November 2014, 天津科学技术出版社 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108575714A (en) * | 2018-05-17 | 2018-09-28 | 湖南省农业环境生态研究所 | A kind of ciliate desert-grass spore germination and intensive seedling production method |
CN108575714B (en) * | 2018-05-17 | 2023-01-06 | 湖南省农业环境生态研究所 | Centipede spore germination and intensive seedling raising method |
CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
CN111096173B (en) * | 2020-01-06 | 2021-09-14 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104770279B (en) | Senecio cineraria plug seedling culture transplanting method | |
CN105993775A (en) | Culture method of Emmenopterys henryi | |
CN107896851A (en) | A kind of live fast numerous method of bletilla earth culture | |
CN107155654A (en) | A kind of passion fruit implantation methods | |
CN103467199B (en) | A kind of Eschscholtzia californica seedling medium and application | |
CN103125386B (en) | Industrial horseradish planting method | |
CN105594533A (en) | Paddy rice seedling culture method of selenium-enriched rice | |
CN107567926A (en) | A kind of fig and asparagus interplanting method | |
CN103694039B (en) | The light cultivation matrix of Hubei Province, a kind of river Camellia fraternal breeding and container seedling culture method thereof | |
CN102986422A (en) | Cultivating method of vitex rotundifolia | |
CN103283476A (en) | Slow release fertilizer seedling culture method for Larix principis-rupprechtii container seedlings | |
CN103039366B (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
CN104429553A (en) | Production method for raising blueberry seedlings by cutting through containers | |
CN109247156B (en) | Method for regulating and controlling flowering phase of hippeastrum rutilum in south China | |
CN107258267A (en) | A kind of implantation methods of plastic tent cucumber | |
CN106613160A (en) | Spore breeding method for arsenic hyperaccumulator Pteris cretica | |
CN106613082A (en) | Method for planting peppers | |
CN106489503A (en) | Oil tree peony Clonal regeneration method for culturing seedlings | |
CN115989784A (en) | Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds | |
CN105601386A (en) | Hydroponic culture solution and oversummer culture method for cabbage type rape microspore seedlings | |
CN105493819A (en) | Factory-like rapid cultivation method for dicrano pteris pedata seedlings | |
CN105660282B (en) | A kind of method that buta-buta is quickly afforested | |
CN104429843A (en) | Chrysalidocarpus lutescens soilless cultivation method | |
CN105494009A (en) | Seeding reproduction cultivating method of Chinese banyan | |
CN105557509A (en) | Cold-hardiness breeding method for camellia |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170510 |