CN110226518B - Tissue culture and rapid propagation method of saxifrage - Google Patents
Tissue culture and rapid propagation method of saxifrage Download PDFInfo
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- CN110226518B CN110226518B CN201910641302.XA CN201910641302A CN110226518B CN 110226518 B CN110226518 B CN 110226518B CN 201910641302 A CN201910641302 A CN 201910641302A CN 110226518 B CN110226518 B CN 110226518B
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- 241001647091 Saxifraga granulata Species 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 21
- 239000012883 rooting culture medium Substances 0.000 claims abstract description 21
- 230000006698 induction Effects 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims description 34
- 241000196324 Embryophyta Species 0.000 claims description 12
- 241000415040 Anemone rivularis Species 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 7
- 230000000249 desinfective effect Effects 0.000 claims description 7
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 7
- 235000007711 Panicum palmifolium Nutrition 0.000 claims description 4
- 241000569924 Pinanga maculata Species 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 210000000078 claw Anatomy 0.000 claims description 3
- 244000288377 Saxifraga stolonifera Species 0.000 abstract description 8
- 235000002953 Saxifraga stolonifera Nutrition 0.000 abstract description 8
- 244000153955 Reynoutria sachalinensis Species 0.000 abstract description 5
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 9
- 239000002609 medium Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000242759 Actiniaria Species 0.000 description 1
- 241000243290 Aequorea Species 0.000 description 1
- 241001083548 Anemone Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a tissue culture and rapid propagation method of saxifrage, which comprises the following steps of 1) disinfection; 2) transversely cutting the surface-sterilized explant into 2-6 mm squares along the direction vertical to the midvein, inoculating the explant with leaf back down in an induction culture medium of a culture bottle, and inoculating 10 explants in each bottle; 3) induced adventitious buds; 4) inoculating the induced adventitious bud into a rooting culture medium, culturing for 60-70 days to obtain a root, establishing a saxifraga stolonifera tissue culture rapid propagation system through in-vitro culture of the saxifraga stolonifera, and ensuring that the survival rate reaches over 95 percent; the propagation speed is obviously improved, the seedling consumption of the rare Yi medicine of the giant knotweed is increased, the germplasm resource quantity of the giant knotweed is enlarged, the cost can be reduced, the operation is simple, and the economic benefit is high.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine planting, in particular to a tissue culture and rapid propagation method of saxifrage.
Background
Aequorea febrifuga Anemones rivularis Buch-ham.ex Dc, also called caoyume, Fangqing, Fanglan, is a perennial medicinal plant of Ranunculaceae, and has the effects of clearing away heat and toxic materials, promoting blood circulation, relaxing muscles and tendons, relieving cough, eliminating phlegm, resisting bacteria, resisting cancer and the like. The giant knotweed herb is usually grown on mountainous regions, grass rooms, stream sides or other wetlands or forest edges with the elevation of 1750m to 3300m in Yunnan province. Secondly, the plant is distributed in Sichuan, Guizhou, southwest of Gansu, southeast of Qinghai, southwest of Hubei, western Guangxi, southern Tibet and eastern China, but with the change of environmental conditions, the wild berberian anemone is endangered and died, which causes resource shortage and supply shortage.
The anemone rivularis has low seed setting rate and short seed life under natural conditions, and is easy to lose germination force, and meanwhile, the progeny of the seeds of the anemone rivularis is easy to lose the original seed property and generate variation, so that the pedicel becomes thin, soft, and the flower type becomes small. The original seed characteristics can be maintained by the plant division propagation, no variation occurs, but seedlings are easily infected by diseases and pests in the propagation process, the propagation speed is low, and the urgent demand of the market on the production of the saxifrage is difficult to meet. Therefore, establishing a rapid and efficient saxifrage seedling production technical system has urgent practical significance for the seedling use amount of rare Yi medicine saxifrage and the expansion of the germplasm resource amount of the saxifrage.
Disclosure of Invention
The invention provides a tissue culture and rapid propagation method of saxifrage.
The scheme of the invention is as follows:
a tissue culture and rapid propagation method of saxifrage comprises the following steps:
1) cutting the young leaves of the anemone rivularis on a superclean workbench to be used as explants, and disinfecting the surfaces of the explants by using 75% of alcohol and 5% of sodium hypochlorite;
2) transversely cutting the surface-sterilized explant into 2-6 mm squares along the direction vertical to the midvein, inoculating the explant with leaf back down in an induction culture medium of a culture bottle, and inoculating 10 explants in each bottle;
3) performing dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 20-35 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d and the temperature is 20-28 ℃ to obtain the induced adventitious bud;
4) inoculating the induced adventitious buds into a rooting culture medium, wherein 10 plants are inoculated in each bottle of rooting culture medium, and the seedlings are cultured for 60-70 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d, and the temperature is 20-28 ℃ to obtain the rooting.
Preferably, the induction medium is MS medium containing 1.0-6.0 mg/L, IAA 0.1.1-0.4 mg/L of 6-BA.
As a preferred technical scheme, the rooting culture medium is an MS culture medium containing 0.1-0.3 mg/L of IAA.
As a preferable technical scheme, the illumination intensity in the step 4) is 1500 Lux.
As a preferable technical scheme, the illumination time in the step 4) is 12 h/d.
As a preferable technical scheme, the temperature in the step 4) is 24 ℃.
Preferably, the culture in step 4) is performed for 60 days.
As a preferred technical scheme, the rooting root is 9-11 cm long.
As a preferred technical scheme, each tuber of saxifrage rooted in the step 4) takes root 7-9, the tuber takes the shape of a thick and short chicken claw, the tuber is tall and straight, the height of each tuber of saxifrage is 11-13 cm, and the leaves are light green.
Due to the adoption of the technical scheme, the tissue culture and rapid propagation method of the anemone rivularis comprises the steps of 1) shearing young leaves of the anemone rivularis on a superclean bench to serve as explants, and disinfecting the surfaces of the explants by using 75% alcohol and 5% sodium hypochlorite; 2) transversely cutting the surface-sterilized explant into 2-6 mm squares along the direction vertical to the midvein, inoculating the explant with leaf back down in an induction culture medium of a culture bottle, and inoculating 10 explants in each bottle; 3) performing dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 20-35 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d and the temperature is 20-28 ℃ to obtain the induced adventitious bud; 4) inoculating the induced adventitious buds into a rooting culture medium, wherein 10 plants are inoculated in each bottle of rooting culture medium, and the seedlings are cultured for 60-70 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d, and the temperature is 20-28 ℃ to obtain the rooting.
The invention has the advantages that:
firstly, a saxifraga stolonifera tissue culture rapid propagation system is established through isolated culture of the saxifraga stolonifera, the survival rate is more than 95%, and the establishment of the saxifraga stolonifera tissue culture rapid propagation method solves the problems of rare seeds, low germination rate and low propagation speed of the saxifraga stolonifera, and is suitable for industrialized seedling culture of the saxifraga stolonifera;
the propagation speed is obviously improved, the seedling consumption of the rare Yi medicine of the giant knotweed is increased, the germplasm resource quantity of the giant knotweed is enlarged, the cost can be reduced, the operation is simple, and the economic benefit is high.
Detailed Description
In order to make up for the above disadvantages, the invention provides a tissue culture and rapid propagation method of saxifrage to solve the problems in the background art.
A tissue culture and rapid propagation method of saxifrage comprises the following steps:
1) cutting the young leaves of the anemone rivularis on a superclean workbench to be used as explants, and disinfecting the surfaces of the explants by using 75% of alcohol and 5% of sodium hypochlorite;
2) transversely cutting the surface-sterilized explant into 2-6 mm squares along the direction vertical to the midvein, inoculating the explant with leaf back down in an induction culture medium of a culture bottle, and inoculating 10 explants in each bottle;
3) performing dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 20-35 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d and the temperature is 20-28 ℃ to obtain the induced adventitious bud;
4) inoculating the induced adventitious buds into a rooting culture medium, wherein 10 plants are inoculated in each bottle of rooting culture medium, and the seedlings are cultured for 60-70 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d, and the temperature is 20-28 ℃ to obtain the rooting.
The induction culture medium is an MS culture medium containing 1.0-6.0 mg/L, IAA 0.1.1-0.4 mg/L of 6-BA.
The rooting culture medium is an MS culture medium containing 0.1-0.3 mg/L of IAA.
The illumination intensity in the step 4) is 1500 Lux.
The illumination time in the step 4) is 12 h/d.
The temperature in the step 4) is 24 ℃.
The culture in the step 4) is carried out for 60 days.
The rooting root is 9-11 cm long.
7-9 roots of each tiger palm grass rooted in the step 4) are in a thick and short chicken claw shape, the plant is tall and straight, the height of each tiger palm grass is 11-13 cm, and the leaves are light green.
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1:
1) cutting the young leaves of the anemone rivularis on a superclean workbench to be used as explants, and disinfecting the surfaces of the explants by using 75% of alcohol and 5% of sodium hypochlorite;
2) transversely cutting the surface-sterilized explants into 2mm squares along the direction vertical to the midvein, inoculating the explants with leaf back down in an induction medium in culture bottles, and inoculating 10 explants in each bottle;
3) performing dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 20 days under the conditions that the illumination intensity is 800Lux, the illumination time is 12h/d and the temperature is 20 ℃ to obtain an induced adventitious bud;
4) inoculating the induced adventitious buds into a rooting culture medium, inoculating 10 plants in each bottle of rooting culture medium, and culturing for 60 days under the conditions of illumination intensity of 800Lux, illumination time of 12h/d and temperature of 20 ℃ to obtain the rooting.
The induction culture medium is MS culture medium containing 6-BA 1.0mg/L, IAA 0.1.1 mg/L.
The rooting culture medium is an MS culture medium containing IAA 0.1 mg/L.
Example 2:
1) cutting the young leaves of the anemone rivularis on a superclean workbench to be used as explants, and disinfecting the surfaces of the explants by using 75% of alcohol and 5% of sodium hypochlorite;
2) transversely cutting the surface-sterilized explants into 6mm squares along the direction vertical to the midvein, inoculating the explants with leaf back down in an induction medium in culture bottles, and inoculating 10 explants in each bottle;
3) carrying out dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 35 days under the conditions that the illumination intensity is 2000Lux, the illumination time is 14h/d and the temperature is 28 ℃ to obtain an induced adventitious bud;
4) inoculating the induced adventitious buds into a rooting culture medium, inoculating 10 plants in each bottle of rooting culture medium, and culturing for 70 days under the conditions of illumination intensity of 2000Lux, illumination time of 14h/d and temperature of 28 ℃ to obtain the rooting.
The induction culture medium is MS culture medium containing 6-BA 6.0mg/L, IAA 0.4.4 mg/L.
The rooting culture medium is an MS culture medium containing IAA 0.3 mg/L.
Example 3:
1) cutting the young leaves of the anemone rivularis on a superclean workbench to be used as explants, and disinfecting the surfaces of the explants by using 75% of alcohol and 5% of sodium hypochlorite;
2) transversely cutting the surface-sterilized explants into 4mm squares in the direction vertical to the midvein, inoculating the explants with leaf back down in an induction medium in culture bottles, and inoculating 10 explants in each bottle;
3) performing dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 30 days under the conditions that the illumination intensity is 1500ux, the illumination time is 12h/d and the temperature is 25 ℃ to obtain an induced adventitious bud;
4) inoculating the induced adventitious buds into a rooting culture medium, inoculating 10 plants in each bottle of rooting culture medium, and culturing for 60 days under the conditions of illumination intensity of 1500Lux, illumination time of 12h/d and temperature of 24 ℃ to obtain the rooting.
The induction culture medium is MS culture medium containing 5.0mg/L, IAA 0.3.3 mg/L6-BA.
The rooting culture medium is an MS culture medium containing IAA 0.2 mg/L.
The length of the root is 10 cm.
Each of the saxifrage plants rooted in the step 4) takes root 8, the plants are tall and straight, the height of each of the saxifrage plants is 12cm, and the leaves are light green.
The rooting rate reaches 100 percent by the embodiment.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. A tissue culture and rapid propagation method of saxifrage is characterized by comprising the following steps:
1) cutting the young leaves of the anemone rivularis on a superclean workbench to be used as explants, and disinfecting the surfaces of the explants by using 75% of alcohol and 5% of sodium hypochlorite;
2) transversely cutting the surface-sterilized explant into 2-6 mm squares along the direction vertical to the midvein, inoculating the explant with leaf back down in an induction culture medium of a culture bottle, and inoculating 10 explants in each bottle; the induction culture medium is an MS culture medium containing 1.0-6.0 mg/L, IAA 0.1.1-0.4 mg/L of 6-BA;
3) performing dark treatment on the explant inoculated in a culture bottle for 3 days, and then culturing for 20-35 days under the conditions that the illumination intensity is 800-2000 Lux, the illumination time is 12-14 h/d and the temperature is 20-28 ℃ to obtain the induced adventitious bud;
4) inoculating the induced adventitious buds into a rooting culture medium, wherein 10 strains are inoculated in each bottle of rooting culture medium, and the adventitious buds are cultured for 60-70 days under the conditions of illumination intensity of 800-2000 Lux, illumination time of 12-14 h/d and temperature of 20-28 ℃ to obtain roots; the rooting culture medium is an MS culture medium containing 0.1-0.3 mg/L of IAA.
2. The tissue culture and rapid propagation method of saxifrage as claimed in claim 1, characterized in that: the illumination intensity in the step 4) is 1500 Lux.
3. The tissue culture and rapid propagation method of saxifrage as claimed in claim 1, characterized in that: the illumination time in the step 4) is 12 h/d.
4. The tissue culture and rapid propagation method of saxifrage as claimed in claim 1, characterized in that: the temperature in the step 4) is 24 ℃.
5. The tissue culture and rapid propagation method of saxifrage as claimed in claim 1, characterized in that: the culture in the step 4) is carried out for 60 days.
6. The tissue culture and rapid propagation method of saxifrage as claimed in claim 1, characterized in that: the rooting root is 9-11 cm long.
7. The tissue culture and rapid propagation method of saxifrage as claimed in claim 1, characterized in that: 7-9 roots of each tiger palm grass rooted in the step 4) are in a thick and short chicken claw shape, the plant is tall and straight, the height of each tiger palm grass is 11-13 cm, and the leaves are light green.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6264998B1 (en) * | 2000-03-01 | 2001-07-24 | Dabur Research Foundation | Extracting betulinic acid from Ziziphus jujuba |
| CN101390496A (en) * | 2008-09-28 | 2009-03-25 | 华中农业大学 | Vitro quick di-wu breeding method |
| CN104450748A (en) * | 2014-11-18 | 2015-03-25 | 西南大学 | Asperugo procumbens delta6-fatty acid desaturase ApD6D gene family as well as recombinant expression vector and application thereof |
| CN105168610A (en) * | 2015-08-26 | 2015-12-23 | 朱小芬 | Composition for cold cough and preparation method thereof |
| CN108451141A (en) * | 2018-04-22 | 2018-08-28 | 南京正宽医药科技有限公司 | A kind of facial mask with moisture-keeping functions |
| CN109608263A (en) * | 2018-12-27 | 2019-04-12 | 天津亚德尔生物质科技股份有限公司 | A kind of preparation method of charcoal selenium fertilizer |
| CN110214561A (en) * | 2019-07-22 | 2019-09-10 | 楚雄师范学院 | A kind of quick division propagation method of brook anemone |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3830960B1 (en) * | 2005-08-12 | 2006-10-11 | 建仁 河乃 | Hair growth substance |
| CN101810143B (en) * | 2010-04-29 | 2012-06-13 | 华中农业大学 | Rapid in-vitro propagation method and application of anemone flaccida fr.schmidt |
| CN104784331A (en) * | 2015-04-30 | 2015-07-22 | 宋彬 | Traditional Chinese medicine for treating dilated cardiomyopathy and preparation method thereof |
-
2019
- 2019-07-16 CN CN201910641302.XA patent/CN110226518B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6264998B1 (en) * | 2000-03-01 | 2001-07-24 | Dabur Research Foundation | Extracting betulinic acid from Ziziphus jujuba |
| CN101390496A (en) * | 2008-09-28 | 2009-03-25 | 华中农业大学 | Vitro quick di-wu breeding method |
| CN104450748A (en) * | 2014-11-18 | 2015-03-25 | 西南大学 | Asperugo procumbens delta6-fatty acid desaturase ApD6D gene family as well as recombinant expression vector and application thereof |
| CN105168610A (en) * | 2015-08-26 | 2015-12-23 | 朱小芬 | Composition for cold cough and preparation method thereof |
| CN108451141A (en) * | 2018-04-22 | 2018-08-28 | 南京正宽医药科技有限公司 | A kind of facial mask with moisture-keeping functions |
| CN109608263A (en) * | 2018-12-27 | 2019-04-12 | 天津亚德尔生物质科技股份有限公司 | A kind of preparation method of charcoal selenium fertilizer |
| CN110214561A (en) * | 2019-07-22 | 2019-09-10 | 楚雄师范学院 | A kind of quick division propagation method of brook anemone |
Non-Patent Citations (2)
| Title |
|---|
| "An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.)";Sediva, J等;《HORTICULTURAL SCIENCE 》;20171107;第44卷(第4期);第186–194页 * |
| "中国—喜马拉雅草玉梅繁殖生物学及生态适应研究";中国植物学会系统与进化植物学专业委员会、云南省植物学会;《2011年全国系统与进化植物学暨第十届青年学术研讨会论文集》;20111026;第2页 * |
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