CN102369881B - Rapid propagation technique of Dendrobium candidum axillary buds - Google Patents
Rapid propagation technique of Dendrobium candidum axillary buds Download PDFInfo
- Publication number
- CN102369881B CN102369881B CN201110284479.2A CN201110284479A CN102369881B CN 102369881 B CN102369881 B CN 102369881B CN 201110284479 A CN201110284479 A CN 201110284479A CN 102369881 B CN102369881 B CN 102369881B
- Authority
- CN
- China
- Prior art keywords
- dendrobium candidum
- buds
- axillalry bud
- propagation
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a rapid propagation technique of Dendrobium candidum axillary buds. According to the technique, Dendrobium candidum axillary buds are used as explants directly to induce fasciculated buds; influences of combinations of different types of hormones with different concentration and different natural extracts on survival and growth of axillary buds, induction and multiplication of fasciculated buds and seedlings are compared, the quantity and quality of seedlings growing from fasciculated buds are researched and explored, the optimal culture medium formula in each stage is screened, and therefore, a fasciculated-bud-way high-efficiency rapid propagation system is established, so that plants obtained through rapid propagation are consistent with parent plants genetically so as to provide basis for restoration of wild population of Dendrobium candidum, and furthermore, wild resources are protected. Compared with vegetative propagation, the rapid propagation technique provided by the invention greatly improves propagation factor. A high-efficiency rapid propagation system of Dendrobium candidum which is an endangered plant provides a technical probability for the large-scale production and is beneficial to the restoration of wild population.
Description
Technical field
The present invention relates to the quick propagating technology of a Plants, be specially a kind of quick propagating technology of dendrobium candidum axillalry bud, belong to biological technical field.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) or (Dendrobium candidum Wall ex Lind.), have another name called ribbed hedyotis herb, is the orchid family (Orchidaceae).The perennial herbaceous plant that grows nonparasitically upon another plant of Dendrobium, often grows nonparasitically upon another plant on thick forest trunk or rock, and normal and bryophyte association.Its fresh stem or dry stem are used as medicine, and are the bulk drugs of the most frequently used traditional medicine of China and Chinese patent drug.It is Tiepi Fengdou that < < Chinese pharmacopoeia > > regulation dendrobium candidum can be processed into earrings dendrobium after softened by baking is dry.Its slightly sweet flavor, cold nature, hinders Feng for the moon and loses, dry polydipsia, food is few retches, weak after being ill, and order is secretly not clear, is one of Chinese ten immortal grass.In modern pharmacological research, it is reported dendrobium candidum also have hypoglycemic, build up resistance and antitumaous effect.
The seed of dendrobium candidum is very tiny, and embryonic development is incomplete, and without endosperm tissue, under nature, germination rate is extremely low, and sexual propagation is difficulty very.Grow in the moon place of overhanging cliff, natural production is very rare, due to very worthy; for a long time always in excessively excavating situation; its wild resource is endangered, by country, is classified as 2 grades of protective plants, and wild resource can not meet the needs in medicinal material market far away.Artificial propagation mainly, by vegetative propagation, constantly tillers or take root and grow new plant from top eustipes part, but output is very low from root, and still the demand from market differs greatly.In order to accomplish that protection and exploitation develop simultaneously, conventionally utilize the method Fast-propagation that tissue is cultivated to obtain seedling, for artificial cultivation provides provenance.Forefathers to the report of dendrobium candidum Fast-propagation in, focus mostly on that to take seed, stem apex, stem section etc. be explant, and breed seedling by the propagation of protocorm with differentiation.The problem wherein existing is likely to produce variation with the offspring of seminal propagation; Stem apex is as the explant maternal plant that can scatter and disappear, and stem section, owing to self carrying disease germs seriously, can be sterilized not thorough.And there are some researches show the particularity due to the stem of noble dendrobium, in the tissue of the stem of noble dendrobium is cultivated, the selection of explant is very crucial factor, different draws materials position and period, the result of cultivating is also different, and the test-tube plantlet that other explants form is faster than seed test-tube plantlet strain, and stem is thick, Miao Zhuan, easily survives.And the axillalry bud that directly has drawn from is as explant, not only draw materials more convenient, and do not hinder the stem of maternal plant, can retain the integrality of stem.At present dendrobium candidum axillalry bud is directly cultivated and induced the research of seedling have not been reported.
Summary of the invention
The object of the invention is for a kind of quick propagating technology of dendrobium candidum axillalry bud is provided; thereby set up the efficient rapid propagation system of Multiple Buds approach; make the plant obtaining by Fast-propagation keep the uniformity in heredity with maternal plant; for the recovery of dendrobium candidum wild population provides material, reach the object of protection wild resource.
Object of the present invention can be achieved through the following technical solutions.
A quick propagating technology for dendrobium candidum axillalry bud, concrete steps are as follows:
1) axillalry bud survival and growth
When dendrobium candidum plants stems internode newly grows the long 0.5～1.0cm of axillalry bud, with blade, carefully cut complete axillalry bud, running water is rinsed well.On superclean bench, material is first used after 75% alcohol-pickled 30s, 0.1% mercuric chloride sterilization 10min, and sterile distilled water embathes 4 times, each 5min, excessive moisture blots with aseptic filter paper.Finally material is seeded on medium.Take MS as minimal medium, add respectively the NAA of 0.3mg/L, the 6-RA of 1.0mg/L, 3% sucrose and the agar of 7g/L, regulating pH value is 5.5～6.0, autoclaving 20～25min.Culturing room's temperature (25 ± 3) ℃, take plant cultivation light as light source, intensity of illumination 2000lx, illumination every day 12h.Cultivate just visible axillalry bud chap in 10 days, elongation, after 60 days, length can reach 1.8～2.0cm, grows 5 leaves, and survival rate is 100%.
2) induction of Multiple Buds and propagation
When axillalry bud continues elongation growth, its base portion constantly has new Multiple Buds to occur.Axillalry bud is transplanted to induction and the propagation of carrying out Multiple Buds on proliferated culture medium.This culture medium prescription is the NAA of MS, 1.0mg/L, the BA of 2.5mg/L, 20% bananas juice, 3% sucrose and the agar of 7g/L, and regulating pH value is 5.5～6.0, autoclaving 20～25min.Culturing room's temperature (25 ± 3) ℃, take plant cultivation light as light source, intensity of illumination 2000lx, illumination every day 12h.Cultivate 160d, propagation multiple can reach 18.0, and along with the prolongation of incubation time, propagation multiple is also and improves gradually trend.
3) Multiple Buds is taken root and is grown seedling
Multiple Buds is transferred to and on root media, carried out culture of rootage, and root media is that NAA, 20% bananas juice and 2.0 ‰ the activated carbon of 1/2MS, 0.2～0.5mg/L mixes.Cultivate 30d, the aerial root of the seedling base portion of growing thickly is flourishing, nearly 20～30 of root quantity, and on root system, adularescent is Powdered, bottle seedling leaf light, and stem is thick, and joint is many, favourable transplanting.
4) acclimatization and transplants
(1) hardening: bottle seedling being placed in green house for 7～10 days before transplanting of taking root cultivated to hardening.
(2) group training transplantation of seedlings: group training transplantation of seedlings matrix is that tongue is fresh, pine bark, wood chip and crushed stone and slightly river sand mix.With 600 times of dilutions of methyl Tobe Feng pulvis, transplanting medium spray disinfectant is transplanted seedlings after 2 days again.
Method transplanting survival rate is up to more than 95% thus.
In order further to improve the reproduction coefficient of Multiple Buds, the domestication of taking Multiple Buds to cultivate without hormone: get high-quality stem of noble dendrobium Multiple Buds bunch and be placed on the domestication medium that hormone-content is higher and cultivate 40d, therefrom choose the bud bunch group that growth is fast; Be placed in again on the domestication medium that hormone-content is lower and cultivate 30d, therefrom choose the bud bunch group that growth is fast; Finally be placed in without cultivating 30d on hormone culture-medium, therefrom choose the fast and healthy and strong bud bunch of growth.Above cultivation temperature is (25 ± 3) ℃.
The dendrobium candidum Multiple Buds bunch that preparation technology of the present invention produces can be for the preparation of sterile dry.Concrete preparation method is: collect bud bunch, remove medium, with 110 ℃ of dry 15min, then 80 ℃, 24h oven dry, then use Co 60 radiation sterilization, finally obtains dendrobium candidum axenic dry powder.
Mentioned reagent of the present invention, except specified otherwise, is mass percent.
Technical scheme of the present invention, has following beneficial effect with respect to prior art: compare with division propagation, greatly improved reproduction coefficient.The large-scale production that is established as of endangered plants dendrobium candidum high efficiency quick breeding system provides technical possibility.Its far reaching significance is not only that the seedling of large-scale production relies on artificial cultivation to meet the need of market; produce economic benefit; and scale reproductive success can also increase wild population quantity, be conducive to the recovery of wild population, produce good social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, further set forth technical characterstic of the present invention.
1) axillalry bud survival and growth
When dendrobium candidum plants stems internode newly grows the long 0.5～1.0cm of axillalry bud, with blade, carefully cut complete axillalry bud, running water is rinsed well.On superclean bench, material is first used after 75% alcohol-pickled 30s, 0.1% mercuric chloride sterilization 10min, and sterile distilled water embathes 4 times, each 5min, excessive moisture blots with aseptic filter paper.Finally material is seeded on medium.Take MS as minimal medium, add respectively the NAA of 0.3mg/L, the 6-RA of 1.0mg/L, 3% sucrose and the agar of 7g/L, regulating pH value is 5.5～6.0, autoclaving 20～25min.Culturing room's temperature (25 ± 3) ℃, take plant cultivation light as light source, intensity of illumination 2000lx, illumination every day 12h.Cultivate just visible axillalry bud chap in 10 days, elongation, after 60 days, length can reach 1.8～2.0cm, grows 5 leaves, and survival rate is 100%.
2) induction of Multiple Buds and propagation
When axillalry bud continues elongation growth, its base portion constantly has new Multiple Buds to occur.Axillalry bud is transplanted to induction and the propagation of carrying out Multiple Buds on proliferated culture medium.This culture medium prescription is the NAA of MS, 1.0mg/L, the BA of 2.5mg/L, 20% bananas juice, 3% sucrose and the agar of 7g/L, and regulating pH value is 5.5～6.0, autoclaving 20～25min.Culturing room's temperature (25 ± 3) ℃, take plant cultivation light as light source, intensity of illumination 2000lx, illumination every day 12h.Cultivate 160d, propagation multiple can reach 18.0, and along with the prolongation of incubation time, propagation multiple is also and improves gradually trend.
3) Multiple Buds is taken root and is grown seedling
Multiple Buds is transferred to and on root media, carried out culture of rootage, and root media is that NAA, 20% bananas juice and 2.0 ‰ the activated carbon of 1/2MS, 0.2～0.5mg/L mixes.Cultivate 30d, the aerial root of the seedling base portion of growing thickly is flourishing, nearly 20～30 of root quantity, and on root system, adularescent is Powdered, bottle seedling leaf light, and stem is thick, and joint is many, favourable transplanting.
4) acclimatization and transplants
(1) hardening: bottle seedling being placed in green house for 7～10 days before transplanting of taking root cultivated to hardening.
(2) group training transplantation of seedlings: group training transplantation of seedlings matrix is that tongue is fresh, pine bark, wood chip and crushed stone and slightly river sand mix.With 600 times of dilutions of methyl Tobe Feng pulvis, transplanting medium spray disinfectant is transplanted seedlings after 2 days again.
Method transplanting survival rate is up to more than 95% thus.
The domestication that Multiple Buds is cultivated without hormone
Get high-quality stem of noble dendrobium Multiple Buds bunch and be placed on the domestication medium that hormone-content is higher and cultivate 40d, therefrom choose the bud bunch group that growth is fast; Be placed in again on the domestication medium that hormone-content is lower and cultivate 30d, therefrom choose the bud bunch group that growth is fast; Finally be placed in without cultivating 30d on hormone culture-medium, therefrom choose the fast and healthy and strong bud bunch of growth.Above cultivation temperature is (25 ± 3) ℃.
The fast traditional font of setting up by Multiple Buds approach is, also makes the quantity of dendrobium candidum high-quality test-tube plantlet be greatly improved, and on suitable medium, the maximum of inducing clumping bud propagation multiple can reach 18.0.With propagation multiple 18.0, calculate, if 1 year subculture of 1 bud is cultivated 5 times, can obtain 185=1889568 bud, cultivate 100 axillalry buds and just can obtain the Multiple Buds of 100,000,000 left and right, and then can obtain 10,000,000,000 clumps of test-tube plantlets.Compare with division propagation, greatly improved reproduction coefficient.The large-scale production that is established as of endangered plants dendrobium candidum high efficiency quick breeding system provides technical possibility.Its far reaching significance is not only that the seedling of large-scale production relies on artificial cultivation to meet the need of market; produce economic benefit; and scale reproductive success can also increase wild population quantity, be conducive to the recovery of wild population, produce good social benefit and ecological benefits.
Multiple Buds nutrient component is identified
Get a part of bud bunch and carry out the mensuration of polysaccharide and stem of noble dendrobium alkaloid equal size.Determination of polysaccharide adopts phenolsulfuric acid method, and with interference compositions such as contained monose, oligosaccharide in 80% alcohol extract determinand, then water extracts polysaccharose composition.Polysaccharose, under effect of sulfuric acid, is first hydrolyzed into monose molecule and dewaters and generate alditol derivative rapidly, is then condensed into colored compound with phenol, with colorimetric method for determining polyoses content.
Because dendrobine lacks ultraviolet absorption peak, so the mensuration of alkaloid be take dendrobine sterling as standard, adopt bromocresol green method to measure.Assay result shows that polysaccharide, alkaloid, amino acid and micronutrient levels and the wild Dendrodium of Multiple Buds is very nearly the same.
Claims (2)
1. a method for quickly breeding for dendrobium candidum axillalry bud, is characterized in that: concrete steps are as follows:
1) axillalry bud survival and growth: when dendrobium candidum plants stems internode newly grows the long 0.5～1.0cm of axillalry bud, cut complete axillalry bud with blade, running water is rinsed well; On superclean bench, material is first used after 75% alcohol-pickled 30s, 0.1% mercuric chloride sterilization 10min, and sterile distilled water embathes 4 times, each 5min, excessive moisture blots with aseptic filter paper; Finally material is seeded on medium; Culturing room's temperature is 22-28 ℃, take plant cultivation light as light source, intensity of illumination 2000lx, illumination every day 12h; Cultivate 10 days, axillalry bud chap, elongation, after 60 days, length reaches 1.8～2.0cm, grows 5 leaves;
2) induction of Multiple Buds and propagation: when axillalry bud continues elongation growth, its base portion constantly has new Multiple Buds to occur; Axillalry bud is transplanted to induction and the propagation of carrying out Multiple Buds on proliferated culture medium; The temperature 22-28 ℃ of culturing room, take plant cultivation light as light source, intensity of illumination 2000lx, illumination every day 12h; Cultivate 160 days;
3) Multiple Buds is taken root and is grown seedling: Multiple Buds is transferred on root media and carried out culture of rootage, cultivate 30d, the aerial root of the seedling base portion of growing thickly is flourishing, nearly 20～30 of root quantity, and on root system, adularescent is Powdered, bottle seedling leaf light, stem is thick, and joint is many;
4) acclimatization and transplants: (1) hardening: bottle seedling being placed in green house for 7～10 days before transplanting of taking root cultivated to hardening; (2) group training transplantation of seedlings: group training transplantation of seedlings matrix is that tongue is fresh, pine bark, wood chip and crushed stone and slightly river sand mix; With 600 times of dilutions of thiophanate methyl pulvis, transplanting medium spray disinfectant is transplanted seedlings after 2 days again;
Medium described in step 1) is to take MS as minimal medium, adds respectively the NAA of 0.3mg/L, the 6-BA of 1.0mg/L, and 3% sucrose and the agar of 7g/L mix, and regulating pH value is 5.5～6.0, autoclaving 20～25min;
Step 2) described proliferation culture medium formula is the NAA of MS, 1.0mg/L, the BA of 2.5mg/L, 20% bananas juice, 3% sucrose and the agar of 7g/L mix, and regulating pH value is 5.5～6.0, autoclaving 20～25min;
Root media described in step 3) is that the NAA, 20% bananas juice of 1/2MS, 0.2～0.5mg/L and 2.0 ‰ activated carbon mix.
2. the method for quickly breeding of the described dendrobium candidum axillalry bud of claim 1, is characterized in that: the dendrobium candidum Multiple Buds of producing is bunch for the preparation of sterile dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110284479.2A CN102369881B (en) | 2011-09-22 | 2011-09-22 | Rapid propagation technique of Dendrobium candidum axillary buds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110284479.2A CN102369881B (en) | 2011-09-22 | 2011-09-22 | Rapid propagation technique of Dendrobium candidum axillary buds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102369881A CN102369881A (en) | 2012-03-14 |
| CN102369881B true CN102369881B (en) | 2014-05-07 |
Family
ID=45789515
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110284479.2A Active CN102369881B (en) | 2011-09-22 | 2011-09-22 | Rapid propagation technique of Dendrobium candidum axillary buds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102369881B (en) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613082A (en) * | 2012-03-31 | 2012-08-01 | 南京农业大学 | Modified medium for improving propagation of stems of Dendrobium officinale and propagation method |
| CN102960226B (en) * | 2012-12-07 | 2014-07-16 | 农定霖 | Dendrobium officinale Kimura et Migo tissue culture seedling acclimatization method |
| CN102972299B (en) * | 2012-12-10 | 2014-06-25 | 黄怀毅 | Rapid propagation method for dendrobium officinale stem tissue culture |
| CN103609447A (en) * | 2013-11-27 | 2014-03-05 | 苏州田园农业技术开发有限公司 | Propagation method of Dendrobium officinale |
| CN103609446A (en) * | 2013-11-27 | 2014-03-05 | 苏州田园农业技术开发有限公司 | Dendrobium officinale propagation method capable of shortening vegetative propagation cycle of Dendrobium officinale |
| CN105475128B (en) * | 2014-09-16 | 2019-05-24 | 长沙学院 | A kind of method of dendrobium candidum tubers in vitro |
| CN104206282A (en) * | 2014-10-10 | 2014-12-17 | 江苏神草生物科技有限公司 | Culture medium for dendrobium tissue cultivation |
| CN104303765B (en) * | 2014-10-10 | 2017-05-31 | 泉州惠安长圣生物科技有限公司 | The high-yield planting method of the stem of noble dendrobium |
| CN104322373A (en) * | 2014-11-12 | 2015-02-04 | 柳州市天姿园艺有限公司 | Dendrobium officinale tissue culture seedling method for propagating by using pedicel |
| CN105613292A (en) * | 2016-01-06 | 2016-06-01 | 江苏远鹏基因组研究院有限公司 | Special dendrobium officinale leaf sheath tissue culture seedling forming method |
| CN106171995A (en) * | 2016-07-20 | 2016-12-07 | 徐伟明 | A kind of culture medium increasing Herba Dendrobii tillering ability |
| CN106258981A (en) * | 2016-08-31 | 2017-01-04 | 耿马四方生物科技开发有限责任公司 | Candidum tissue culturing factorial seedling-culturing method |
| CN107347649A (en) * | 2017-08-11 | 2017-11-17 | 佛山科学技术学院 | A kind of cultural method of dendrobium candidum explant |
| CN109793691A (en) * | 2019-02-27 | 2019-05-24 | 苏州天成新农生物科技有限公司 | Filter method suitable for dendrobium candidum tender shoots stoste |
| CN109793692A (en) * | 2019-02-27 | 2019-05-24 | 苏州天成新农生物科技有限公司 | Filter method suitable for the dry bud stoste of dendrobium candidum |
| CN110521540A (en) * | 2019-09-25 | 2019-12-03 | 盐城工学院 | Improve the matrix and preparation, application method of clavus dendrobe tissue culture seedling hardening survival rate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358427A (en) * | 2001-12-18 | 2002-07-17 | 上海南江衡和生物科技有限公司 | Ironbark dendrobe sprout and preparation process and use thereof |
| CN101810140A (en) * | 2009-09-03 | 2010-08-25 | 四川万安石斛产业开发有限公司 | Method for propagating dendrobium candidum test-tube plantlets |
-
2011
- 2011-09-22 CN CN201110284479.2A patent/CN102369881B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358427A (en) * | 2001-12-18 | 2002-07-17 | 上海南江衡和生物科技有限公司 | Ironbark dendrobe sprout and preparation process and use thereof |
| CN101810140A (en) * | 2009-09-03 | 2010-08-25 | 四川万安石斛产业开发有限公司 | Method for propagating dendrobium candidum test-tube plantlets |
Non-Patent Citations (6)
| Title |
|---|
| 何涛等.铁皮石斛腋芽的快速繁殖.《中国野生植物资源》.2010,第29卷(第1期), |
| 关萍等.叠鞘石斛茎段组织培养与花芽诱导.《时珍国医国药》.2009,第20卷(第1期), |
| 叠鞘石斛茎段组织培养与花芽诱导;关萍等;《时珍国医国药》;20091231;第20卷(第1期);第205-206页 * |
| 郭洪波等.铁皮石解茎节离体培养的研究.《时珍国医国药》.2007,第18卷(第11期), |
| 铁皮石斛腋芽的快速繁殖;何涛等;《中国野生植物资源》;20100228;第29卷(第1期);第58-61页 * |
| 铁皮石解茎节离体培养的研究;郭洪波等;《时珍国医国药》;20071231;第18卷(第11期);第2659-2660页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102369881A (en) | 2012-03-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102369881B (en) | Rapid propagation technique of Dendrobium candidum axillary buds | |
| CN103651121B (en) | A kind of bletilla differentiation, strong seedling culture base | |
| CN102499090B (en) | Method for isolated culture of Haworthia succulent plants | |
| CN102301952B (en) | Method for breeding chamomile | |
| CN101965800B (en) | Tissue culture breeding method for metabriggsia ovalifolia | |
| CN103651122B (en) | A kind of bletilla protocorm induction medium | |
| CN103931492A (en) | Tissue-culture rapid seedling growing method for apple rootstock M9 | |
| CN101491214B (en) | Pinellia tuber artificial seed stem production method | |
| CN101637096B (en) | Quick and high-efficiency breeding method for cephalotaxus hainanensis seed and seedling | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN102246694A (en) | Tissue culture method of gynura divaricata | |
| CN105766654B (en) | A kind of Nanchuan jackfruit method for tissue culture | |
| CN105379624B (en) | A kind of tissue culture and rapid propagation method of Eucalyptus pellita | |
| CN101965796B (en) | Method for performing tissue culture and rapid propagation on primula saxatilis | |
| CN102907326B (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| CN101953300B (en) | Tissue culture method for Curcuma wenyujin No.1 | |
| CN102273405B (en) | Cultivating method of Hosta plantaginea Gold Standard | |
| CN102893872A (en) | Tissue culture method for domesticated seedlings of iris pallida | |
| CN103168692A (en) | Salix saposhnikovii tissue culture method | |
| CN105475129A (en) | Tissue-culture rapid propagation method for arundina graminifolia | |
| CN104686329A (en) | Tissue culture method for Eucommia ulmoides Oliv. | |
| CN101637130B (en) | Cephalotaxus hainanensis embryo culturing and seedling breeding method | |
| CN101810144A (en) | Rapid breeding method of senecio cruentus | |
| CN103548695B (en) | A kind of meadowrueleaf corydalis root quick breeding method for tissue culture | |
| CN102144569A (en) | Method for rapidly propagating root and rhizome of Divaricate Gynura |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160929 Address after: 200090 Shanghai city Yangpu District Gongqing Road No. 400 in Dragon Technology Patentee after: Shanghai XCT Health Food Co., Ltd. Address before: 200082, room 9, No. 296, Anshan branch, Yangpu District, Shanghai Patentee before: Cheng Siyuan Biotechnology (Shanghai) Co., Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181203 Address after: 2000 90 Zhonghailong Science and Technology, No. 400 Gongqing Road, Yangpu District, Shanghai Patentee after: Cheng Siyuan Biotechnology (Shanghai) Co., Ltd. Address before: 2000 90 Zhonghailong Science and Technology, No. 400 Gongqing Road, Yangpu District, Shanghai Patentee before: Shanghai XCT Health Food Co., Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190327 Address after: 2000 90 Zhonghailong Science and Technology, No. 400 Gongqing Road, Yangpu District, Shanghai Patentee after: Shanghai Xiancaotang Biotechnology Co., Ltd. Address before: 2000 90 Zhonghailong Science and Technology, No. 400 Gongqing Road, Yangpu District, Shanghai Patentee before: Cheng Siyuan Biotechnology (Shanghai) Co., Ltd. |