CN110521540A - Improve the matrix and preparation, application method of clavus dendrobe tissue culture seedling hardening survival rate - Google Patents
Improve the matrix and preparation, application method of clavus dendrobe tissue culture seedling hardening survival rate Download PDFInfo
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- CN110521540A CN110521540A CN201910908585.XA CN201910908585A CN110521540A CN 110521540 A CN110521540 A CN 110521540A CN 201910908585 A CN201910908585 A CN 201910908585A CN 110521540 A CN110521540 A CN 110521540A
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- clavus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The formula that the present invention discloses a kind of matrix for improving clavus dendrobe tissue culture seedling hardening survival rate includes: fat tree skin, wood fragments bits, wood shavings and rubble, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:1.5~2:1:1.5~2.Hardening is carried out to clavus dendrobium nobile using the matrix, it can make clavus Rapid Propagation of Dendrobium, improve breeding coefficient, and it is not subject to seasonal restrictions, hardening high survival rate, the wood fragments bits added in matrix effectively improve the whole permeability of matrix, after enzymolysis processing, further improve the specific surface area and adsorption capacity of this four kinds of substances, the retain water and nutrients ability of matrix is enhanced, has established good environmental basis for the growth of clavus dendrobium nobile, addition rubble increases the gas permeability of matrix, the growth for being conducive to clavus dendrobium nobile, takes root；Fat tree micromicro is added to provide nutritional ingredient, while excellent adsorption for the growth of clavus dendrobium nobile.
Description
Technical field
The invention belongs to field of plant cell engineering technology, and in particular to a kind of raising clavus dendrobe tissue culture seedling hardening survives
The matrix of rate and preparation, application method.
Background technique
Clavus dendrobium nobile (Dendrobium findlayanum Par.et Rchb.f.) is the perennial plant of orchid family Dendrobium
Object, happiness is shady and cool, has higher ornamental and medical value.Its pseudobulb not branch has several sections of, internode flat bar shape, due to its false squama
The special shape of stem, is similar to the bodily form of hornet, so also known as wasp waist dendrobium nobile.Originate in Yunnan Province of China south, Burma, Thailand
(height above sea level 800-1000m).March at florescence, the flower service life about 3 weeks.A large amount of artificial growth is carried out at present, the source of seedling is main
It is wild species, modes of reproduction is budding and cuttage.This kind of modes of reproduction due to without sexual propagation and plant inner virus and
Pathogen, which constantly accumulates, leads to the quality of seedling and plant worse and worse.As the quantity of artificial growth is more and more, seedling
Source also gradually will become tissue-cultured seedling from wild species, the important stage that the hardening of tissue-cultured seedling will also be to provide seedling and survive, and
The selection of matrix is even more important.
Therefore, it is necessary to provide, a kind of growth cycle is short, breeding is quick, it is high, at low cost to transplant survival rate and can improve stick
The matrix of arthrolith dry measure used in former times training tissue culture seedling survival rate.
Summary of the invention
That the technical problem to be solved in the present invention is to provide a kind of growth cycles is short, breeding quickly, transplanting survival rate is high, cost
Matrix low and that clavus dendrobe tissue culture seedling hardening survival rate can be improved.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: the raising clavus dendrobe tissue culture seedling hardening at
The formula of the matrix of motility rate includes: fat tree skin, wood fragments bits, wood shavings and rubble, the fat tree skin, wood fragments bits, wood shavings and rubble
Volume ratio are as follows: 1:1.5~2:1:1.5~2.Hardening is carried out to clavus dendrobium nobile using the matrix, clavus dendrobium nobile can be made quickly numerous
It grows, improves breeding coefficient, and be not subject to seasonal restrictions, hardening high survival rate, the wood fragments bits added in matrix effectively improve matrix
Whole permeability further improve the specific surface area and adsorption capacity of this four kinds of substances after enzymolysis processing, enhance
The retain water and nutrients ability of matrix has established good environmental basis for the growth of clavus dendrobium nobile, and addition rubble increases matrix
Gas permeability, be conducive to the growth of clavus dendrobium nobile, take root；Be added to fat tree micromicro with for clavus dendrobium nobile growth provide nutrition at
Point, while excellent adsorption.
As the preferred technical solution of the present invention, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:2:
1:2.By lot of experiment validation, using the matrix hardening survival rate highest of the formula of the ratio, breeding potential highest.
A further improvement of the present invention is that the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate also wraps
Include folium artemisiae argyi dry powder, the folium artemisiae argyi dry powder account for the matrix total volume of the raising clavus dendrobe tissue culture seedling hardening survival rate 0.5~
1%.On the one hand addition folium artemisiae argyi dry powder can sterilize prevents tissue-cultured seedling rotten, on the other hand can provide battalion to the growth of clavus dendrobium nobile
It supports, increases taking root for clavus dendrobium nobile.
A further improvement of the present invention is that the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate also wraps
It includes and spends treasured, it is described to spend the 0.01~0.02% of the precious matrix total volume for accounting for the raising clavus dendrobe tissue culture seedling hardening survival rate.Add
Add and spend the precious nutritional ingredient for improving matrix, so that the growth for clavus dendrobe tissue culture seedling provides the battalion easily absorbed filled and organized
It supports.
A further improvement of the present invention is that it is described spend it is precious include spend precious No. 1 and spend it is No. 2 precious, it is described spend precious No. 1 with it is described
Spending precious No. 2 volume ratios is 1:1.5~3.By lot of experiment validation, using the matrix of the ratio spent precious No. 1 with spend treasured 2
Hardening survival rate highest, breeding potential highest.
That the technical problem to be solved in the present invention is to provide a kind of growth cycles is short, breeding quickly, transplanting survival rate is high, cost
The preparation method of matrix low and that clavus dendrobe tissue culture seedling hardening survival rate can be improved.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: the raising clavus dendrobe tissue culture seedling hardening at
The preparation method of the matrix of motility rate: by fat tree skin, wood fragments bits, wood shavings, rubble, folium artemisiae argyi dry powder and treasured is spent to use after mixing.
Using technical solution of the present invention, the beneficial effect generated is:
1, gas permeability is strong, good water absorption, is conducive to the growth of tissue-cultured seedling；
2, the quality of seedling ensure that as fungicide using natural component；
3, each ingredient is easier to obtain, at low cost.
Specific embodiment
Embodiment 1: the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate include: fat tree skin, wood fragments bits,
Wood shavings and rubble, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:2:1:2.
Embodiment 2: the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate include: fat tree skin, wood fragments bits,
Wood shavings and rubble, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:1.5:1:1.5.
Embodiment 3: the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate include: fat tree skin, wood fragments bits,
Wood shavings and rubble, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:1.5:1:2.
Embodiment 4: the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate include: fat tree skin, wood fragments bits,
Wood shavings and rubble, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:2:1:1.5.
Embodiment 5: the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate include: fat tree skin, wood fragments bits,
Wood shavings, rubble and folium artemisiae argyi dry powder, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:2:1:2, the folium artemisiae argyi are dry
Powder accounts for the 1% of the matrix total volume of the raising clavus dendrobe tissue culture seedling hardening survival rate, i.e., the described fat tree skin, wood fragments bits, wood shavings,
The volume ratio of rubble and folium artemisiae argyi dry powder are as follows: 16.5:32.5:16.5:32.5:1.
Embodiment 6: the formula of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate include: fat tree skin, wood fragments bits,
Wood shavings, rubble, folium artemisiae argyi dry powder and treasured is spent, the volume ratio of the fat tree skin, wood fragments bits, wood shavings and rubble are as follows: 1:2:1:2, it is described
Folium artemisiae argyi dry powder accounts for the 0.98% of the matrix total volume of the raising clavus dendrobe tissue culture seedling hardening survival rate, described that treasured is spent to account for the raising
The 0.02% of the matrix total volume of clavus dendrobe tissue culture seedling hardening survival rate；The i.e. described fat tree skin, wood fragments bits, wood shavings, rubble, Chinese mugwort
Leaf dried powder and the volume ratio for spending treasured are as follows: 16.5:32.5:16.5:32.5:0.98:0.02；Wherein spending precious includes spending precious No. 1 and flower
No. 2 precious, Hua Bao 1 is 1:3 with the volume ratio for spending treasured 2.
The preparation method of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate: by fat tree skin, wood fragments bits, wood shavings,
Rubble, folium artemisiae argyi dry powder and spending precious uses after mixing.
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (6)
1. a kind of matrix for improving clavus dendrobe tissue culture seedling hardening survival rate, which is characterized in that the raising clavus dendrobe tissue culture seedling
The formula of the matrix of hardening survival rate includes: fat tree skin, wood fragments bits, wood shavings and rubble, the fat tree skin, wood fragments bits, wood shavings and
The volume ratio of rubble are as follows: 1:1.5~2:1:1.5~2.
2. the matrix according to claim 1 for improving clavus dendrobe tissue culture seedling hardening survival rate, which is characterized in that described thick
The volume ratio of bark, wood fragments bits, wood shavings and rubble are as follows: 1:2:1:2.
3. the matrix according to claim 1 for improving clavus dendrobe tissue culture seedling hardening survival rate, which is characterized in that the raising
The formula of the matrix of clavus dendrobe tissue culture seedling hardening survival rate further includes folium artemisiae argyi dry powder, and the folium artemisiae argyi dry powder accounts for the raising clavus stone
The 0.5~1% of the matrix total volume of dry measure used in former times training tissue culture seedling survival rate.
4. the matrix according to claim 1 for improving clavus dendrobe tissue culture seedling hardening survival rate, which is characterized in that the raising
The formula of the matrix of clavus dendrobe tissue culture seedling hardening survival rate further includes spending treasured, described that treasured is spent to account for the raising clavus dendrobe tissue culture seedling
The 0.01~0.02% of the matrix total volume of hardening survival rate.
5. the matrix according to claim 4 for improving clavus dendrobe tissue culture seedling hardening survival rate, which is characterized in that the flower
Treasured include spend precious No. 1 and spend it is No. 2 precious, it is described spend precious No. 1 with described to spend precious No. 2 volume ratios be 1:1.5~3.
6. the matrix according to claim 1-5 for improving clavus dendrobe tissue culture seedling hardening survival rate, feature exist
In the preparation method of the matrix of the raising clavus dendrobe tissue culture seedling hardening survival rate: by fat tree skin, wood fragments bits, wood shavings, rubble,
Folium artemisiae argyi dry powder and spending precious uses after mixing.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102369881A (en) * | 2011-09-22 | 2012-03-14 | 澄思源生物科技（上海）有限公司 | Rapid propagation technique of Dendrobium candidum axillary buds |
| CN103718948A (en) * | 2013-12-06 | 2014-04-16 | 广西壮族自治区林业科学研究院 | Cultivation method of new germplasm of ornamental Dendrobium spp. |
| CN103918558A (en) * | 2014-05-04 | 2014-07-16 | 中国热带农业科学院橡胶研究所 | Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle |
| CN107242016A (en) * | 2017-07-31 | 2017-10-13 | 贵州盛达生植物发展有限公司 | A kind of method of bamboo underwood planting dendrobium candidum |
| CN107624610A (en) * | 2017-10-30 | 2018-01-26 | 南京工业大学大丰海洋产业研究院 | A kind of Nandina domestica'Fire power' training tissue culture seedling matrix |
| CN107652062A (en) * | 2017-10-30 | 2018-02-02 | 南京工业大学大丰海洋产业研究院 | A kind of matrix for improving new pteris fern training tissue culture seedling survival rate |
| CN107711413A (en) * | 2017-11-10 | 2018-02-23 | 南京工业大学大丰海洋产业研究院 | A kind of matrix for improving Radix Astragali training tissue culture seedling survival rate |
| CN109526594A (en) * | 2017-07-26 | 2019-03-29 | 北京神飞航天应用技术研究院 | A kind of cultural method of space flight balcony vegetables |
-
2019
- 2019-09-25 CN CN201910908585.XA patent/CN110521540A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102369881A (en) * | 2011-09-22 | 2012-03-14 | 澄思源生物科技（上海）有限公司 | Rapid propagation technique of Dendrobium candidum axillary buds |
| CN103718948A (en) * | 2013-12-06 | 2014-04-16 | 广西壮族自治区林业科学研究院 | Cultivation method of new germplasm of ornamental Dendrobium spp. |
| CN103918558A (en) * | 2014-05-04 | 2014-07-16 | 中国热带农业科学院橡胶研究所 | Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle |
| CN109526594A (en) * | 2017-07-26 | 2019-03-29 | 北京神飞航天应用技术研究院 | A kind of cultural method of space flight balcony vegetables |
| CN107242016A (en) * | 2017-07-31 | 2017-10-13 | 贵州盛达生植物发展有限公司 | A kind of method of bamboo underwood planting dendrobium candidum |
| CN107624610A (en) * | 2017-10-30 | 2018-01-26 | 南京工业大学大丰海洋产业研究院 | A kind of Nandina domestica'Fire power' training tissue culture seedling matrix |
| CN107652062A (en) * | 2017-10-30 | 2018-02-02 | 南京工业大学大丰海洋产业研究院 | A kind of matrix for improving new pteris fern training tissue culture seedling survival rate |
| CN107711413A (en) * | 2017-11-10 | 2018-02-23 | 南京工业大学大丰海洋产业研究院 | A kind of matrix for improving Radix Astragali training tissue culture seedling survival rate |
Non-Patent Citations (2)
| Title |
|---|
| 杨念等: "羚羊石斛的离体快速繁殖", 《植物生理学通讯》 * |
| 杨明志等: "《中国药用石斛标准研究与应用》", 31 December 2012, 四川科学技术出版社 * |
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Application publication date: 20191203 |