CN105706881A - Little pepper fast propagation method - Google Patents
Little pepper fast propagation method Download PDFInfo
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- CN105706881A CN105706881A CN201610148589.9A CN201610148589A CN105706881A CN 105706881 A CN105706881 A CN 105706881A CN 201610148589 A CN201610148589 A CN 201610148589A CN 105706881 A CN105706881 A CN 105706881A
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- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a little pepper fast propagation method, comprising the following steps: (1) preparing a cuttage bed; (2) preparing a rooting powder solvent: taking rooting powder, placing in a beaker of 500ml, adding less alcohol, and adding distilled water to prepare a solution after the rooting powder is dissolved; (3) cutting branches: selecting a pepper branch with a diameter of 0.4-0.5cm, cutting into stem sections of 4-5cm, keeping one leaf for each stem section when cutting, flatly cutting the part, away from the leaf by 1cm, at the upper opening of each stem section, and flatly cutting the lower opening of each steam section; (4) sterilizing the branch: soaking the well cut stem sections in 50% of Azoxystrobin solutions of 1500 times for sterilizing; (5) rooting; (6) performing cuttage; (7) managing after the cuttage. According to the method, characters of a stock plant variety are not changed, fast and efficiency propagation is realized, operation is simple and the cost is low.
Description
Technical field
The invention belongs to technical field of plant propagation, be specifically related to a kind of capsicum method for quickly breeding.
Background technology
Capsicum typically uses seminal propagation (sexual propagation).But for some rare pepper resources, as from the external Fructus Capsici cenospecies introduced or newfound male sterile plant etc., as used sexual propagation, their hereditary character will be changed, makes these rare pepper resources to preserve.
The method using seminal propagation generally comprises following two:
First method: utilize seed and MS culture medium to carry out tissue culture expanding propagation method
The method be seed 75% ethanol and 0.1% mercuric chloride are processed 30s and 3min respectively after, clean by sterile water wash, be then seeded in containing appropriate 6-BA, NAA(or GA), IAA, AgNO3Aseptic enrichment culture is carried out, it is thus achieved that a large amount of aseptic seedling with in the MS culture medium of the material such as caseinhydrolysate.Again aseptic seedling is cut off at hypocotyl, be seeded in the MS culture medium containing materials such as appropriate NAA, IAA and activated carbons and carry out root culture, after adventitious root germinates, then transplant after seedling exercising 1-2 week.From being seeded into rooting and transplant, about need 80d.
The shortcoming of the method is: 1) with after seed disinfection, then cultivates in MS culture medium, it is thus achieved that the further expanding propagation of aseptic seedling, this method is still that sexual propagation, is not pure line cultivar such as seed, can cause the change of characters of progenies；2) carry out aseptic culture in group training room, need a series of equipment and the various medicines such as sterilizing room, super quiet workbench, high-pressure sterilizing pot, analytical balance, culturing room, put into relatively big, operate the most more complicated；3) cycle is long, and from the beginning of seed disinfection, to taking root, Seedling is transplanted, and about needs 80d.
Second method: utilize seed and B5 medium to carry out tissue culture expanding propagation method
Seed with 75% alcohol disinfecting pepper seed 30s, then is sterilized 20-25min by the method with sodium hypochlorite (effective chlorine 1.1%), with distilled water flushing clean after, it is seeded in B5 without on hormone culture-medium, after axillalry bud germinates, in units of axillalry bud, it is cut into several sections, is seeded in containing appropriate IAA, GA3、AgNO3Carry out aseptic enrichment culture with on the B5 medium of the material such as lactoalbumin hydrolysate, when seedling of taking root grows up to 3-3.5cm, transplant after seedling exercising 2-3.From being seeded into rooting and transplant, need 70-80d.
The shortcoming of the method is: 1) with after seed disinfection, then cultivates in B5 medium, it is thus achieved that the further expanding propagation of aseptic seedling.This method is still that sexual propagation, is not pure line cultivar such as seed, can cause the change of characters of progenies；2) carry out aseptic culture in group training room, need a series of equipment and the various medicines such as sterilizing room, super quiet workbench, high-pressure sterilizing pot, analytical balance, culturing room, put into relatively big, operate the most more complicated；3) cycle is long: from the beginning of seed disinfection, and to taking root, Seedling is transplanted, and about needs 80d.
If employing asexual propagation, then the character of these materials or plant intactly can be preserved.Cottage propagation is one of vegetative mode of plant, is by intercepting one section of plant nutrition organ, inserts in the loose soil of moistening, fine sand or other substrate, utilize its regeneration capacity, be allowed to take root and branch out, become new plant.Although it is not very strong that capsicum produces the ability of adventitious root, but by certain density HORMONE TREATMENT, Caulis Capsici segment cuttage can be made to regenerate, those rare pepper resources are allowed to be bred and to preserve.
Existing capsicum asexual reproduction method is common cuttage, and the method is in the time of the year when autumn changes into winter, and Fructus Capsici branch is cut into 16-18cm, and upper end is cut into flat mouth, and lower end is cut into angle, removes blade and only stays petiole.Then soak 2-3h with the copper-bath of 1%, pull out and clean with clear water, put natural air drying in the cool.Being layered in wooden case or other container by clean wet river sand, fill deep 6-8cm, plug by 3.3cm and handle ground branch well, the degree of depth is suitable to expose a leaf segment outside.Also can be cutting medium with wood sawdust.Long make new advances root and sprouting after 15-20 days.The shortcoming of the method is: cutting used by 1) is oversize, and the cutting affecting breeding coefficient: 16-18cm wants 3-4 to save, and the quantity that each Fructus Capsici maternal plant can be cut the long branch of 16-18cm is the most limited, so greatly reduces the breeding coefficient of Fructus Capsici；2) the slotting machine degree of depth is 6-8cm, and the substrate amount of consumption is too big, time-consuming；3) 1% copper-bath processes cutting as antibacterial, and because copper ion has certain toxicity, the concentration of 1% is far above contact threshold value 0.5mg/m3, want SC during operation, careless slightly, operator can be damaged；4) 1% copper-bath processes cutting as antibacterial, because copper ion has certain toxicity, repeatedly cleans cutting after process with clear water, if any residual, easily cutting clip is produced injury, affects cutting survival rate；5) time of the year when autumn changes into winter starts cuttage, and temperature is relatively low, affects rooting of cuttings and survives.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of capsicum method for quickly breeding, does not change maternal plant varietal character, simple to operation, low cost.
For solving above-mentioned technical problem, the technical solution used in the present invention is: a kind of capsicum method for quickly breeding, comprises the steps:
1) slotting machine prepares: is placed in the cavities of plastics cave dish as cultivation matrix by perlite, after hole tray fills perlite, strikes off suitable for reading for cavities, put hole tray in turn, irrigated by the pearlite interstitial substance in hole tray with sterilized water, make the moisture content of substrate reach 35-40%；
2) preparation of rooting powder solution: weigh specially good effect root-inducing powder, puts in the beaker of 500ml, adds a small amount of ethanol, after root-inducing powder dissolves, adds distilled water and is made into rooting powder solution；In this rooting powder solution, the weight ratio of specially good effect root-inducing powder, ethanol and distilled water is 1:5:150；
3) shearing of branch: select the Fructus Capsici branch of a diameter of 0.4-0.5cm, is cut into the stem section of a length of 4-5cm, and during shearing, each stem section retains a piece of blade, and stem section is suitable for reading in truncation away from blade 1cm, stem section end opening truncation；
4) sterilization treatment of branch: the stem section cut is substantially immersed in 1500 times of Fluoxastrobin solution of 50% and carries out sterilizing process, pull out after 10s and dry；
5) rooting treatment: the stem pars infrasegmentalis after sterilization treatment is immersed step 2) in the rooting powder solution for preparing, pull out after 4h and dry, prepare cuttage；
6) cuttage: be slightly coarser than chopsticks or the garden wooden stick of stem section diameter with 75% alcohol disinfecting both hands and diameter, chopsticks or garden wooden stick are inserted perpendicularly in the middle of the cavities of hole tray, forming cuttage cavities, the degree of depth of cuttage cavities is 2-4cm, is then inserted one by one in cuttage cavities by the end opening of stem section；After stem section is inserted, press substrate with hands, make stem section and the substrate contiguity of insertion；
7) management after cuttage: spray slotting machine with sterilized water after being plugged, a good bow member, cover plastic sheeting and sunshade net, the stem section of cuttage is placed in without in the environment of direct light, temperature is maintained at 23-27 DEG C, and the moisture content of pearlite interstitial substance is maintained at 35-40%；Every 10 days with 50% 1500 times of Fluoxastrobin solution sprayings once, within 30-35 days, grow adventitious root, in 7-10 days, gradually throw off sunshade net, increase illumination and ventilation carries out seedling exercising, treat that new root length degree reaches 1-2cm, can transplant；
Throw off sunshade net method particularly includes: during 7-10 days, by time every day only early, evening throw off sunshade net, at the period covered with sunshade net that 10:00-15:00 sunlight is strong, be gradually transitions whole day the most not covered with sunshade net.
Further, using 50 hole dishes in described step (1), in hole tray, the height of cavities is 4.1-4.5cm.
Further, described root-inducing powder is the specially good effect root-inducing powder containing 90wt% naphthalene acetic acid that Wuwei County, Anhui Province flower fertilizer factory produces.
Further, the cutting medium employed in step (1) is original perlite, according to used perlite as substrate, then needs the sterilization first carrying out substrate to process.
Further, in described step (3), the stem section after shearing is one group with 10, after end opening alignment, is bundled by stem pars infrasegmentalis by cotton cord or plastic ties, and during binding, blade is outwardly, bundlees not loosen for degree.
Further, described step 7) regulates temperature by the size of shed vent, temperature is adjusted to 25 DEG C most widely suited.
Further, in described step 7), after cuttage, the frequency of water spray and the number of water spray, to determine according to the situation of dry weather degree and leaves water loss shrinkage；If dry weather, blade has shrinkage situation, sprays a water every day, makes the moisture content of substrate reach 35-40%；If wet weather, substrate do not does, and blade is straight and upright shrink-proof, the most every other day water of spray.
Beneficial effects of the present invention:
1, the merit of maternal plant is kept: because carrying out cottage propagation with Fructus Capsici branch is asexual propagation, such propagation method will not change the merit of maternal plant so that rare pepper resource is preserved.
2, with low cost, easy to operate: because being made without tissue culture propagation, can carry out in common booth even indoor, have only to prepare the materials such as ethanol, root-inducing powder, platform balance, beaker, knife blade, Fluoxastrobin, sterilized water, watering pot, plastic foil, sunshade net, with low cost, easy to operate.
3, the breeding cycle is short: just can transplant after above-mentioned cottage propagation, about 30-40d, do sth. in advance a month seedling than tissue cultured seedling.
4, reproductive efficiency is high: because cutting is short, only one joint band one leaf, and the strain Fructus Capsici that grows up can cut 20-30 stem section, and breeding coefficient is higher.
Detailed description of the invention
A kind of capsicum method for quickly breeding of the present invention, comprises the steps:
1) slotting machine prepares: be placed in the cavities of 50 hole plastics cave dishes as cultivation matrix by perlite, the height of cavities is 4.1-4.5cm, after hole tray fills perlite, strike off suitable for reading for cavities, put hole tray in turn, with sterilized water, the pearlite interstitial substance in hole tray is irrigated, make the moisture content of substrate reach 35-40%；As selected used perlite to do cutting medium, then re-use after substrate must be carried out sterilization treatment.
2) preparation of rooting powder solution: claim the 1g specially good effect root-inducing powder (production of Wuwei County, Anhui Province flower fertilizer factory) containing 90wt% naphthalene acetic acid, put in the beaker of 500ml, add 5ml ethanol, after root-inducing powder dissolves, adds 150g distilled water and is made into rooting powder solution；Caulis Capsici section generation adventitious root indifferent, needs to use rooting powder solution to carry out rooting treatment, and the concentration of rooting powder solution wants suitable, and too low too high all can the impact of concentration is taken root.
3) shearing of branch: select the Fructus Capsici branch of a diameter of 0.4-0.5cm, is cut into the stem section of a length of 4-5cm, and during shearing, each stem section retains a piece of blade, and stem section is suitable for reading in truncation away from blade 1cm, stem section end opening truncation；For ease of sterilization and rooting treatment, branch bottom, after end opening alignment, can be bundled by cotton cord or plastic ties, not injured blade by blade outwardly during binding by 10 stem Duan Weiyi groups；Binding is the tightest, not loosen for degree；
When shearing Fructus Capsici branch, sharp knife blade, truncation should be used, the smooth of otch must be noted.Can not cut sth. askew, the wound cut sth. askew is relatively big, is unfavorable for the healing of wound and takes root.Each branch is that a joint carries a leaf, and blade wants complete, and healthy and strong blade can provide carbon nutrition by photosynthesis, is conducive to seedling of taking root.
4) sterilization treatment of branch: the stem section cut is substantially immersed in 1500 times of Fluoxastrobin solution of 50% and carries out sterilizing process, pull out after 10s and dry；
5) rooting treatment: the stem pars infrasegmentalis after sterilization treatment is immersed step 2) in the rooting powder solution for preparing, pull out after 4h and dry, prepare cuttage；The time of rooting treatment is wanted suitably, it is impossible to long, can not be too short；
6) cuttage: be slightly coarser than chopsticks or the garden wooden stick of stem section diameter with 75% alcohol disinfecting both hands and diameter, chopsticks or garden wooden stick are inserted perpendicularly in the middle of the cavities of hole tray, forming cuttage cavities, the degree of depth of cuttage cavities is 2-4cm, is then inserted one by one in cuttage cavities by the end opening of stem section；After stem section is inserted, press substrate with hands, make stem section and the substrate contiguity of insertion；It is noted that the directivity of branch during cuttage, it is impossible to turn upside down；
7) management after cuttage: spray slotting machine with sterilized water after being plugged, prop up good bow member, cover plastic sheeting and sunshade net, the stem section of cuttage is placed in without in the environment of direct light, regulate temperature by the size of shed vent, keep the temperature at 23-27 DEG C (most widely suited with 25 DEG C)；Water spray frequency and water spray number, be according to dry weather degree and the situation of leaves water loss shrinkage, such as dry weather, blade has shrinkage situation, every day to spray a water, the water yield with squirt substrate for degree, make the moisture content of pearlite interstitial substance be maintained at such as 35-40%；If wet weather, substrate do not does, and blade is straight and upright shrink-proof, sprays the least water the most every other day.In order to prevent and treat cutting seedling by infection process, within one time 30-35 days, grew adventitious root every 10 days with 1500 times of Fluoxastrobin solution sprayings of 50%, in 7-10 days, gradually throw off sunshade net, increase illumination and ventilation carries out seedling exercising, treat that new root length degree reaches 1-2cm, can transplant.
Throw off sunshade net method particularly includes: during 7-10 days, by time every day only early, evening throw off sunshade net, at the period covered with sunshade net that 10:00-15:00 sunlight is strong, be gradually transitions whole day the most not covered with sunshade net.
The method using the present invention does not change maternal plant varietal character, it is possible to rapidly and efficiently breed, simple to operate, low cost.Using 10-20 times that propagation method reproductive efficiency is existing propagation method of the present invention, cost only has 1/3rd of existing propagation method, the survival rate after transplanting to 90%.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; the principle that the present invention is simply described described in above-described embodiment and description; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within scope of the claimed invention.Claimed scope is defined by appending claims and equivalent thereof.
Claims (7)
1. a capsicum method for quickly breeding, it is characterised in that: comprise the steps:
1) slotting machine prepares: is placed in the cavities of plastics cave dish as cultivation matrix by perlite, after hole tray fills perlite, strikes off suitable for reading for cavities, put hole tray in turn, irrigated by the pearlite interstitial substance in hole tray with sterilized water, make the moisture content of substrate reach 35-40%；
2) preparation of rooting powder solution: weigh root-inducing powder, puts in the beaker of 500ml, adds a small amount of ethanol, after root-inducing powder dissolves, adds distilled water and is made into rooting powder solution；In this rooting powder solution, the weight ratio of root-inducing powder, ethanol and distilled water is 1:5:150；
3) shearing of branch: select the Fructus Capsici branch of a diameter of 0.4-0.5cm, is cut into the stem section of a length of 4-5cm, and during shearing, each stem section retains a blade, and stem section is suitable for reading in truncation away from blade 1cm, stem section end opening truncation；
4) sterilization treatment of branch: the stem section cut is substantially immersed in 1500 times of Fluoxastrobin solution of 50% and carries out sterilizing process, pull out after 10s and dry；
5) rooting treatment: the stem pars infrasegmentalis after sterilization treatment is immersed step 2) in the rooting powder solution for preparing, pull out after 4h and dry, prepare cuttage；
6) cuttage: be slightly coarser than chopsticks or the garden wooden stick of stem section diameter with 75% alcohol disinfecting both hands and diameter, chopsticks or garden wooden stick are inserted perpendicularly in the middle of the cavities of hole tray, forming cuttage cavities, the degree of depth of cuttage cavities is 2-4cm, is then inserted one by one in cuttage cavities by the end opening of stem section；After stem section is inserted, press substrate with hands, make stem section and the substrate contiguity of insertion；
7) management after cuttage: spray slotting machine with sterilized water after being plugged, a good bow member, cover plastic sheeting and sunshade net, the stem section of cuttage is placed in without in the environment of direct light, temperature is maintained at 23-27 DEG C, and the moisture content of pearlite interstitial substance is maintained at 35-40%；Every 10 days with 50% 1500 times of Fluoxastrobin solution sprayings once, within 30-35 days, grow adventitious root, in 7-10 days, gradually throw off sunshade net, increase illumination and ventilation carries out seedling exercising, treat that new root length degree reaches 1-2cm, can transplant；
Throw off sunshade net method particularly includes: during 7-10 days, by time every day only early, evening throw off sunshade net, at the period covered with sunshade net that 10:00-15:00 sunlight is strong, be gradually transitions whole day the most not covered with sunshade net.
A kind of capsicum method for quickly breeding the most according to claim 1, it is characterised in that: using 50 hole dishes in described step (1), in hole tray, the height of cavities is 4.1-4.5cm.
A kind of capsicum method for quickly breeding the most according to claim 1, it is characterised in that: described root-inducing powder is the specially good effect root-inducing powder containing 90wt% naphthalene acetic acid that Wuwei County, Anhui Province flower fertilizer factory produces.
A kind of capsicum method for quickly breeding the most according to claim 1, it is characterized in that: the cutting medium employed in step (1) is original perlite, according to used perlite as substrate, then the sterilization first carrying out substrate is needed to process.
A kind of capsicum method for quickly breeding the most according to claim 1, it is characterised in that: in described step (3), the stem section after shearing is one group with 10, after end opening alignment, being bundled by stem pars infrasegmentalis by cotton cord or plastic ties, during binding, blade is outwardly, bundlees not loosen for degree.
A kind of capsicum method for quickly breeding the most according to claim 1, it is characterised in that: described step 7) regulates temperature by the size of shed vent, temperature is adjusted to 25 DEG C most widely suited.
A kind of capsicum method for quickly breeding the most according to claim 1, it is characterised in that: in described step 7), after cuttage, the frequency of water spray and water spray number to determine according to the situation of dry weather degree and leaves water loss shrinkage；If dry weather, blade has shrinkage situation, sprays a water every day, makes the moisture content of substrate reach 35-40%；If wet weather, substrate do not does, and blade is straight and upright shrink-proof, the most every other day water of spray.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106305397A (en) * | 2016-08-25 | 2017-01-11 | 合肥市田然农业科技园有限公司 | Soilless culture method of catharanthus roseus |
| CN106900473A (en) * | 2017-04-27 | 2017-06-30 | 东营黄蓝知识产权运营管理有限公司 | Europe Lee's epicormic branch cutting method |
| CN108464135A (en) * | 2018-03-20 | 2018-08-31 | 南通科技职业学院 | A kind of efficient capsicum Technique on Cuttage Propagation |
| CN108770610A (en) * | 2018-05-25 | 2018-11-09 | 攸县华盛蔬菜种植专业合作社 | A kind of implantation methods of capsicum |
| CN109258469A (en) * | 2018-11-19 | 2019-01-25 | 海南大学 | A kind of method of Chinese Capsicum Cotyledons with petiole regeneration induction plant |
| CN112840880A (en) * | 2021-01-11 | 2021-05-28 | 郑本霞 | Method for maintaining sterile plants of hot peppers |
| CN114303665A (en) * | 2022-01-17 | 2022-04-12 | 河北省农林科学院经济作物研究所 | Sugar-reducing pepper water culture cutting rooting method |
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| CN109258469A (en) * | 2018-11-19 | 2019-01-25 | 海南大学 | A kind of method of Chinese Capsicum Cotyledons with petiole regeneration induction plant |
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