CN104472199A - Cuttage propagation method for anisetree bark - Google Patents
Cuttage propagation method for anisetree bark Download PDFInfo
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- CN104472199A CN104472199A CN201410841544.0A CN201410841544A CN104472199A CN 104472199 A CN104472199 A CN 104472199A CN 201410841544 A CN201410841544 A CN 201410841544A CN 104472199 A CN104472199 A CN 104472199A
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- seedbed
- anisetree bark
- cuttage
- cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a cuttage propagation method for anisetree bark. The method comprises the following steps: a, selection and treatment of cutting slips: selecting robust branches, cutting into cutting slips, soaking the base parts of the cutting slips into disinfecting liquid for 30 minutes, and soaking in a rooting inductive agent for 2 hours; b, preparation of a substrate and a seedbed: selecting a uniform mixture of peat soil, perlite, vermiculite and fine river sand for serving as a cutting substrate, disinfecting the cutting substrate with disinfecting liquid, and making a seedbed of 15 centimeters in thickness; c, cottage, obliquely cutting the cutting slips into the seedbed substrate by 7-10 centimeters; d, management of the seedbed after cuttage: spraying water onto the seedbed in the morning and in the evening each day, spraying 500-time thiophanate methyl seven days later once every week for two times in total, spraying 3/4MS nutrient liquid one week later, and culturing till rooting and seedling emergence. By adopting the cuttage propagation method in breeding of anisetree bark seedlings, the breeding speed of the anisetree bark is increased, and the seedling breeding and scale planting of the anisetree bark are ensured greatly.
Description
Technical field
The present invention relates to a kind of cuttage breeding method of anisetree bark.
Background technology
Anisetree bark (Illicium difengpi K.L.B.et K.I.M.) is Magnoliaceae Illicium plant, has another name called Cortex schizophragmatis integrifolii radicis, Chinese hydrangeavine root-bark etc.Anisetree bark is a kind of special product traditional Chinese medicine in EXPLOITATION AND UTILIZATION IN GUANGXI KARST, is used as medicine with bark, warm in nature, taste is pungent, puckery, has and dispels rheumatism, the effects such as promoting qi circulation and relieving pain, having good curative effect to diseases such as Fall injuries, lumbar muscle strain and rheumatic arthritis, is the main raw material(s) of multiple Chinese patent drug product.Anisetree bark mainly originates in all peaces of Southwestern Kwangsi, China, Mashan, Debao to Longzhou and other places.
Anisetree bark Regional Distribution is narrow and reserves rare.Along with riseing year by year of market demand, anisetree bark wild resource suffers over-exploitation, is on the verge of exhaustion, lists " Chinese Plants Red Data Book " vulnerable species in.Under natural conditions, anisetree bark is mainly bred with seed, but because of its seed coat thin, grease deterioration time dry, perishable again when crossing wet, very easily lose germinating capacity, bring extreme difficulties to scientific research and introducing and planting.And cottage propagation can Fast-propagation is a large amount of in a short time anisetree bark seedling, but also have concurrently and keep maternal merit and the low advantage of cost.Prior art is not for the report of anisetree bark cuttage breeding method.
Summary of the invention
The present invention has designed and developed a kind of cuttage breeding method of anisetree bark.The present invention adopts cottage method to breed anisetree bark seedling, to improve the reproduction speed of anisetree bark, for the sapling multiplication of anisetree bark and large-scale planting provide sound assurance.
Technical scheme provided by the invention is:
A cuttage breeding method for anisetree bark, the method comprises the following steps:
A) selection of cuttings and process:
A1) select healthy and strong branch, be cut into cuttings;
A2) pull out after the base portion of described cuttings being soaked 30min in thimerosal, dry in the air in the cool to surface and do not drip;
A3) described cuttings base portion is placed in after 2h is soaked in root induction agent pulls out, slightly dry in the air, drain surperficial water droplet;
B) preparation in matrix and seedbed: cutting medium selects peat soil, perlite, the homogeneous mixture of vermiculite and thin river sand, makes the seedbed that thickness is 15cm with thimerosal after carrying out disinfection;
C) cuttage: described cuttings is tilted 40 ~ 50 ° of cuttages in described medium of seedling bed, the degree of depth is 7-10cm;
D) management in seedbed after cuttage: every day sprays water to described seedbed sooner or later, sprays the thiophanate methyl of 500 times, spray weekly once, spray twice altogether after 7 days, sprinkling 3/4MS nutrient solution after a week, is cultured to and takes root, emerges.
Preferably, in the cuttage breeding method of described anisetree bark, described cuttings is that 2-3 is raw, the thick 0.5-1cm of stem and band axillalry bud.
Preferably, in the cuttage breeding method of described anisetree bark, described cutting length is 10-15cm, has 1 ~ 2 axillalry bud, upper end straight snips, and lower end is tiltedly cut and clip angle at 45 °.
Preferably, in the cuttage breeding method of described anisetree bark, described step a2) in thimerosal adopt 0.3% liquor potassic permanganate.
Preferably, in the cuttage breeding method of described anisetree bark, described step a3) in the composition of root induction agent comprise the methyl α-naphthyl acetate of 100-300mg/L, the gibberellin of 50-200mg/L, the indolebutyric acid of 200-800mg/L, ABT No. 1 root-inducing powder, the chlormequat of 100-500mg/L and the vitamin C of 50-100mg/L of 10-50mg/L.
Preferably, in the cuttage breeding method of described anisetree bark, described step b) in peat soil in cutting medium, perlite, the volume ratio of vermiculite and thin river sand is 1: 1: 1: 1, and thimerosal adopts carbendazim 1000 times of liquid.
Preferably, in the cuttage breeding method of described anisetree bark, described step c) in the cuttings spacing in the rows of cuttage on seedbed be 6-8cm, line-spacing is 10-12cm.
Preferably, in the cuttage breeding method of described anisetree bark, described steps d) in seedbed condition of culture be: temperature 23-28 DEG C, air humidity 70-80%, shade rate 50-60%.
Preferably, in the cuttage breeding method of described anisetree bark, described seedbed first irrigates with water before cuttage, then irrigates with the hydrogen peroxide solution of 0.5%.
The present invention adopts cottage method to breed anisetree bark seedling, wherein adopt the root induction agent process cuttings of autogamy, the enzymic activity of anisetree bark cuttings cells in vivo can be activated, promote the schizogamy of cell, root cell is grown out faster, thus significantly improves the rooting rate of anisetree bark, and the present invention is environmentally friendly, easy and simple to handle, have concurrently simultaneously and keep maternal merit and the low advantage of cost, there is stronger practicality and promotional value.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but should not limit the scope of the invention with this.
Embodiment 1
Select raw, the thick 0.5-1cm of stem of 2-3 and the healthy and strong branch of anisetree bark of band axillalry bud, in the cool branch is cut into 10-15cm, has the cuttings of 1-2 axillalry bud, cuttings upper end straight snips, lower end is tiltedly cut, and clip is at 45 °, and otch is smoothly without hair.Potassium permanganate with 0.3% soaks cuttings base portion 30min, pulls out, dries in the air in the cool to surface and do not drip.Cuttings base portion through sterilization is placed in root induction agent (methyl α-naphthyl acetate 100mg/L, gibberellin 50mg/L, indolebutyric acid 200mg/L, ABT No. 1 root-inducing powder 10mg/L, chlormequat 100mg/L, vitamin C 50mg/L) middle immersion 2h, pull out, slightly dry in the air, drain surperficial water droplet.
Cutting medium peat soil, perlite, vermiculite, thin river sand are mixed by volume at 1: 1: 1: 1, carry out disinfection with carbendazim 1000 times of liquid, be made into the seedbed that thickness is 15cm, before cuttage, seedbed first irrigated with water, then irrigate with the hydrogen peroxide solution of 0.5%.Then cuttings is pressed spacing in the rows 6-8cm, line-spacing 10-12cm, tilt 40 ~ 50 ° of cuttages in medium of seedling bed, the degree of depth is 7-10cm.Period, sooner or later water spray, maintenance seedbed condition of culture is: temperature 23-28 DEG C, air humidity 70-80%, shade rate 50-60%.Spray the thiophanate methyl of 500 times after 7 days, spray weekly once, spray twice altogether, after one week, spray 3/4MS nutrient solution.After cuttage 40d, disposable lifting investigation rooting percent is 85.7%.
Embodiment 2
Select raw, the thick 0.5-1cm of stem of 2-3 and the healthy and strong branch of anisetree bark of band axillalry bud, in the cool branch is cut into 10-15cm, has the cuttings of 1-2 axillalry bud, cuttings upper end straight snips, lower end is tiltedly cut, and clip is at 45 °, and otch is smoothly without hair.Potassium permanganate with 0.3% soaks cuttings base portion 30min, pulls out, dries in the air in the cool to surface and do not drip.Cuttings base portion through sterilization is placed in root induction agent (methyl α-naphthyl acetate 200mg/L, gibberellin 100mg/L, indolebutyric acid 400mg/L, ABT No. 1 root-inducing powder 25mg/L, chlormequat 250mg/L, vitamin C 75mg/L) soak time is 2h, pull out, slightly dry in the air, drain surperficial water droplet.
Cutting medium peat soil, perlite, vermiculite, thin river sand are mixed by volume at 1: 1: 1: 1, carry out disinfection with carbendazim 1000 times of liquid, be made into the seedbed that thickness is 15cm, before cuttage, seedbed first irrigated with water, then irrigate with the hydrogen peroxide solution of 0.5%.Then cuttings is pressed spacing in the rows 6-8cm, line-spacing 10-12cm, tilt 40 ~ 50 ° of cuttages in medium of seedling bed, the degree of depth is 7-10cm.Period, sooner or later water spray, maintenance seedbed condition of culture is: temperature 23-28 DEG C, air humidity 70-80%, shade rate 50-60%.Spray the thiophanate methyl of 500 times after 7 days, spray weekly once, spray twice altogether, after one week, spray 3/4MS nutrient solution.After cuttage 40d, disposable lifting investigation rooting percent is 94%.
Embodiment 3
Select raw, the thick 0.5-1cm of stem of 2-3 and the healthy and strong branch of anisetree bark of band axillalry bud, in the cool branch is cut into 10-15cm, has the cuttings of 1-2 axillalry bud, cuttings upper end straight snips, lower end is tiltedly cut, and clip is at 45 °, and otch is smoothly without hair.Potassium permanganate with 0.3% soaks cuttings base portion 30min, pulls out, dries in the air in the cool to surface and do not drip.Cuttings base portion through sterilization is placed in root induction agent (methyl α-naphthyl acetate 300mg/L, gibberellin 200mg/L, indolebutyric acid 800mg/L, ABT No. 1 root-inducing powder 50mg/L, chlormequat 500mg/L, vitamin C 100mg/L) soak time is 2h, pull out, slightly dry in the air, drain surperficial water droplet.
Cutting medium peat soil, perlite, vermiculite, thin river sand are mixed by volume at 1: 1: 1: 1, carry out disinfection with carbendazim 1000 times of liquid, be made into the seedbed that thickness is 15cm, before cuttage, seedbed first irrigated with water, then irrigate with the hydrogen peroxide solution of 0.5%.Then cuttings is pressed spacing in the rows 6-8cm, line-spacing 10-12cm, tilt 40 ~ 50 ° of cuttages in medium of seedling bed, the degree of depth is 7-10cm.Period, sooner or later water spray, maintenance seedbed condition of culture is: temperature 23-28 DEG C, air humidity 70-80%, shade rate 50-60%.Spray the thiophanate methyl of 500 times after 7 days, spray weekly once, spray twice altogether, after one week, spray 3/4MS nutrient solution.After 40d, disposable lifting investigation rooting percent is 92.8%.
In sum, promote in the method for anisetree bark cottage propagation, preferred plan is root induction agent (methyl α-naphthyl acetate 200mg/L anisetree bark cuttings being placed in autogamy, gibberellin 100mg/L, indolebutyric acid 400mg/L, ABT No. 1 root-inducing powder 25mg/L, chlormequat 250mg/L, vitamin C 75mg/L) soak 2h in solution, then in peat soil, perlite, vermiculite, thin river sand=1: cultivate in the matrix of 1: 1: 1 (volume ratio).The anisetree bark rooting percent that the program obtains is high, and reproduction speed is fast, and seedling is healthy and strong.
Although working of an invention scheme is open as above, but it is not restricted to listed in specification and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the proportioning example described.
Claims (9)
1. a cuttage breeding method for anisetree bark, is characterized in that, the method comprises the following steps:
A) selection of cuttings and process:
A1) select healthy and strong branch, be cut into cuttings;
A2) pull out after the base portion of described cuttings being soaked 30min in thimerosal, dry in the air in the cool to surface and do not drip;
A3) described cuttings base portion is placed in after 2h is soaked in root induction agent pulls out, slightly dry in the air, drain surperficial water droplet;
B) preparation in matrix and seedbed: cutting medium selects peat soil, perlite, the homogeneous mixture of vermiculite and thin river sand, makes the seedbed that thickness is 15cm with thimerosal after carrying out disinfection;
C) cuttage: described cuttings is tilted 40 ~ 50 ° of cuttages in described medium of seedling bed, the degree of depth is 7-10cm;
D) management in seedbed after cuttage: every day sprays water to described seedbed sooner or later, sprays the thiophanate methyl of 500 times, spray weekly once, spray twice altogether after 7 days, sprinkling 3/4MS nutrient solution after a week, is cultured to and takes root, emerges.
2. the cuttage breeding method of anisetree bark as claimed in claim 1, is characterized in that, described cuttings is that 2-3 is raw, the thick 0.5-1cm of stem and band axillalry bud.
3. the cuttage breeding method of anisetree bark as claimed in claim 2, it is characterized in that, described cutting length is 10-15cm, has 1 ~ 2 axillalry bud, upper end straight snips, and lower end is tiltedly cut and clip angle at 45 °.
4. the cuttage breeding method of anisetree bark as claimed in claim 1, is characterized in that, described step a2) in thimerosal adopt 0.3% liquor potassic permanganate.
5. the cuttage breeding method of anisetree bark as claimed in claim 1, it is characterized in that, described step a3) in the composition of root induction agent comprise the methyl α-naphthyl acetate of 100-300mg/L, the gibberellin of 50-200mg/L, the indolebutyric acid of 200-800mg/L, ABT No. 1 root-inducing powder, the chlormequat of 100-500mg/L and the vitamin C of 50-100mg/L of 10-50mg/L.
6. the cuttage breeding method of anisetree bark as claimed in claim 1, is characterized in that, described step b) in peat soil in cutting medium, perlite, the volume ratio of vermiculite and thin river sand is 1: 1: 1: 1, and thimerosal adopts carbendazim 1000 times of liquid.
7. the cuttage breeding method of anisetree bark as claimed in claim 1, is characterized in that, described step c) in the cuttings spacing in the rows of cuttage on seedbed be 6-8cm, line-spacing is 10-12cm.
8. the cuttage breeding method of anisetree bark as claimed in claim 1, is characterized in that, described steps d) in seedbed condition of culture be: temperature 23-28 DEG C, air humidity 70-80%, shade rate 50-60%.
9. the cuttage breeding method of anisetree bark as claimed in claim 1, it is characterized in that, described seedbed first irrigates with water before cuttage, then irrigates with the hydrogen peroxide solution of 0.5%.
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Cited By (13)
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| CN105145071A (en) * | 2015-09-06 | 2015-12-16 | 广西壮族自治区药用植物园 | Cutting propagation method of brucea javanica |
| CN105210661A (en) * | 2015-11-12 | 2016-01-06 | 广西壮族自治区药用植物园 | A kind of cuttage breeding method of the lobus cardiacus Japanese ardisia |
| CN105409547A (en) * | 2015-12-01 | 2016-03-23 | 广西壮族自治区药用植物园 | Cutting propagation method for kadsura coccinea |
| CN105900805A (en) * | 2016-01-18 | 2016-08-31 | 北京林业大学 | Paeonia ostii soilless culture technology |
| CN106561425A (en) * | 2016-10-31 | 2017-04-19 | 广西壮族自治区药用植物园 | Cutting propagation method for kmeria septentrionalis |
| CN106804379A (en) * | 2017-03-09 | 2017-06-09 | 广西壮族自治区中国科学院广西植物研究所 | A kind of method for promoting anisetree bark in karst mountain transplant survival |
| CN107242088A (en) * | 2017-07-18 | 2017-10-13 | 福建农林大学 | A kind of cuttage breeding method of that rattan of the five fingers |
| CN107242008A (en) * | 2017-07-18 | 2017-10-13 | 福建农林大学 | A kind of cuttage breeding method of variegated leaf stauntonvine |
| CN107371947A (en) * | 2017-07-18 | 2017-11-24 | 福建农林大学 | A kind of cuttage breeding method of that rattan of caudal lobe |
| CN109220270A (en) * | 2018-11-06 | 2019-01-18 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method based on optimal hormone combination |
| CN110249817A (en) * | 2019-07-30 | 2019-09-20 | 云南省林业科学院 | A kind of cuttage breeding method of rare tree watter milkwort root leaf or bark |
| CN114303816A (en) * | 2021-12-30 | 2022-04-12 | 广西壮族自治区中国科学院广西植物研究所 | Conservation method for karst endangered plant anisetree bark |
| CN115997566A (en) * | 2022-12-22 | 2023-04-25 | 延边大学 | Rapid cutting propagation method for rhodiola sachalinensis |
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|---|---|---|---|---|
| CN105145071A (en) * | 2015-09-06 | 2015-12-16 | 广西壮族自治区药用植物园 | Cutting propagation method of brucea javanica |
| CN105210661A (en) * | 2015-11-12 | 2016-01-06 | 广西壮族自治区药用植物园 | A kind of cuttage breeding method of the lobus cardiacus Japanese ardisia |
| CN105409547B (en) * | 2015-12-01 | 2018-04-03 | 广西壮族自治区药用植物园 | The cuttage breeding method of black tiger |
| CN105409547A (en) * | 2015-12-01 | 2016-03-23 | 广西壮族自治区药用植物园 | Cutting propagation method for kadsura coccinea |
| CN105900805A (en) * | 2016-01-18 | 2016-08-31 | 北京林业大学 | Paeonia ostii soilless culture technology |
| CN106561425A (en) * | 2016-10-31 | 2017-04-19 | 广西壮族自治区药用植物园 | Cutting propagation method for kmeria septentrionalis |
| CN106804379A (en) * | 2017-03-09 | 2017-06-09 | 广西壮族自治区中国科学院广西植物研究所 | A kind of method for promoting anisetree bark in karst mountain transplant survival |
| CN106804379B (en) * | 2017-03-09 | 2020-04-03 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting survival of transplanting anisetree bark in karst rocky mountain |
| CN107242088A (en) * | 2017-07-18 | 2017-10-13 | 福建农林大学 | A kind of cuttage breeding method of that rattan of the five fingers |
| CN107242008A (en) * | 2017-07-18 | 2017-10-13 | 福建农林大学 | A kind of cuttage breeding method of variegated leaf stauntonvine |
| CN107371947A (en) * | 2017-07-18 | 2017-11-24 | 福建农林大学 | A kind of cuttage breeding method of that rattan of caudal lobe |
| CN109220270A (en) * | 2018-11-06 | 2019-01-18 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method based on optimal hormone combination |
| CN110249817A (en) * | 2019-07-30 | 2019-09-20 | 云南省林业科学院 | A kind of cuttage breeding method of rare tree watter milkwort root leaf or bark |
| CN114303816A (en) * | 2021-12-30 | 2022-04-12 | 广西壮族自治区中国科学院广西植物研究所 | Conservation method for karst endangered plant anisetree bark |
| CN115997566A (en) * | 2022-12-22 | 2023-04-25 | 延边大学 | Rapid cutting propagation method for rhodiola sachalinensis |
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