CN105409518A - Introduction and cultivation method for campbell's magnolia - Google Patents
Introduction and cultivation method for campbell's magnolia Download PDFInfo
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- CN105409518A CN105409518A CN201510822836.4A CN201510822836A CN105409518A CN 105409518 A CN105409518 A CN 105409518A CN 201510822836 A CN201510822836 A CN 201510822836A CN 105409518 A CN105409518 A CN 105409518A
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- 238000012364 cultivation method Methods 0.000 title claims abstract description 27
- 241000244483 Magnolia campbellii Species 0.000 title abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 43
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- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 28
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 19
- 238000002791 soaking Methods 0.000 claims abstract description 19
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 14
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- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000003448 gibberellin Substances 0.000 claims abstract description 14
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 14
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- 241000218394 Magnolia liliiflora Species 0.000 claims description 59
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- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 6
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention relates to an introduction and cultivation method for campbell's magnolia and belongs to the technical field of plant cultivation. The method comprises the steps of: pre-treatment: collecting fruits of campbell's magnolia in a fruit mature period, airing the fruits and taking out seeds, and removing oil films of seed coats; germination acceleration: soaking the seeds in a gibberellin acetone solution for 24-72 hours; stratification: burying the seeds in wet sand for stratification; seed soaking: soaking the seeds after stratification with a salicylic acid aqueous solution for 12-36 hours; sowing: hill-seeding the seeds in matrix nutrient soil, wherein the hill-seeding depth is 0.5-2 cm, and controlling the water content of the matrix nutrient soil at 20-25% till seeds germinate; culture of seedlings: putting the seedlings in a shady shed for seedling, and after the seedlings grow to predetermined heights, performing hardening-seedling and out-planting the seedlings. By adopting the method, campbell's magnolia which grows in regions with high elevation, high altitude and relatively low annual average temperature can be successfully introduced to regions with low elevation, low altitude and relatively high annual average temperature.
Description
Technical field
The present invention relates to field of plant growing technology, particularly relate to the introduction and cultivation method of a kind of Yunnan-Tibet lily magnolia.
Background technology
Yunnan-Tibet lily magnolia, formal name used at school is Magnoliacampbellii, is the plant of Magnoliaceae Magnolia, is born in the woodland of height above sea level 2500-3500m, originates in northwestern Yunnan Province (Wei Xi) and western (Ruili), southern Tibet.At present, wild Yunnan-Tibet lily magnolia is rare in the western regions of the Yunnan Province, and except the precious Taishan, Yongping County National Park has ancient tree and grows, Binchuan, Eryuan County also only have within the border and distribute on a small quantity, sporadicly, are the rare tree that the world is rare.The flowers are in blossom for it as lotus, and rose pink is to wind rose and aubergine or white, and delicate fragrance imprisons fragrance, and the florescence, prior to leaf growth, winter Mo to early spring was open, is one of ornamental tree species of good city and courtyard greening very early, was more described as " god sets " by Tibetan.
At present few to the research of Yunnan-Tibet lily magnolia seed collection, process, storage and seeding and seedling raising technology, yet there are no pertinent literature to Yunnan-Tibet lily magnolia to low latitudes, the report of height above sea level, average annual temperature district's introducing and planting higher.
Summary of the invention
Based on this, the invention provides the introduction and cultivation method of a kind of Yunnan-Tibet lily magnolia, adopt the method, can will be positioned at the Yunnan-Tibet lily magnolia success introducing and planting of high latitude area to low latitudes such as Xuwens.
An introduction and cultivation method for Yunnan-Tibet lily magnolia, comprises the following steps:
Pre-treatment: the fruit gathering Yunnan-Tibet lily magnolia fruit ripe period, takes out seed after airing, removes seed coat oil film, obtains Yunnan-Tibet lily magnolia seed, for subsequent use;
Vernalization: it is process 24-72 hour in the acetone soln of 500-2000mg/L that above-mentioned Yunnan-Tibet lily magnolia seed is soaked in GA content;
Lamination: seed is embedded in wet sand and carries out Stratificated treatment;
Seed soaking: get the seed after lamination, after cleaning with clear water, take concentration as the aqueous solution of salicylic acid seed soaking 12-36 hour of 0.3-0.7mmol/L;
Sowing: be sowed in Culture media by the Seed Points after above-mentioned seed soaking, the program request degree of depth is 0.5-2cm, the moisture mass percentage controlling Culture media is 20-25%, until seed sprouting;
Nursery: be placed in shading shed by the seedling of germination, keeps ventilated, carries out nursery, grows to after predetermined altitude until seedling, the shade net of shading shed is opened hardening, goes out garden.
Above-mentioned introduction and cultivation method, in order to will High aititude, high latitude, Yunnan-Tibet lily magnolia that average annual temperature is lower be grown on, introduce a fine variety to low latitudes, height above sea level, average annual temperature district higher, as the Xuwen County of Zhanjiang, Guangdong Province, Xuwen County is positioned at the mainland southernmost, tropical the northern fringe, light temperature is sufficient, rainfall is abundant, and temperature warms up, throughout the year frostless.The average annual temperature in the whole county 23.5 DEG C, Extreme Maximum Temperature 38.8 DEG C, Annual lowest climate temperature 2.2 DEG C, year effective active accumulated temperature 8515.4 DEG C, average annual rainfall 1374.9 millimeters.The natural conditions of Xuwen County and the former self-sow environmental condition of Yunnan-Tibet lily magnolia differ greatly, in order to by Yunnan-Tibet lily magnolia Xuwen County success introducing and planting, the present inventor is through a large amount of investigations repeatedly and grope, and obtains above-mentioned introduction and cultivation method.
First this introduction and cultivation method needs to carry out pre-treatment and vernalization to Yunnan-Tibet lily magnolia seed, and select specific solvent---acetone is used as the solvent carrier of vernalization hormone, improve the germination rate of Yunnan-Tibet lily magnolia seed, subsequently by this seed lamination in wet sand, with sowing after aqueous solution of salicylic acid seed soaking, and by the strict water content controlling Culture media, improve the emergence rate of Yunnan-Tibet lily magnolia seed, successfully by the low latitudes originating in Tibet, the seeds in Yunnan have introduced a fine variety the southernmost end of the mainland.
Wherein in an embodiment, in described presprouting of seeds step, first by Yunnan-Tibet lily magnolia seed at normal temperatures with water logging kind 24-72 hour, then this seed be placed in gibberellin acetone soln process.First seed is soaked 1-3 days at normal temperatures, the effect of gibberellin acetone soln vernalization can be strengthened.
Wherein in an embodiment, in described nursery step, when temperature is more than 30 DEG C, sprayed the aqueous solution of salicylic acid of a 0.05-0.15mmol/L every 5-8 days.The present inventor finds in practice, and after Yunnan-Tibet lily magnolia germinates, seedling is feared day with high temperature and to be burnt and arid very much, and its blade often up starts from bottom to engender that jaundice is curling and come off, and after 20 days, major part occurs that the plant of above-mentioned phenomenon is withered dead.Occur in practice that the probability of this kind of situation reaches 50%-60%.For above-mentioned phenomenon, the present inventor finds, as the salicylic acid solution to seedling periodically sprinkle suitable concentration, can well alleviate above-mentioned phenomenon, the probability of leaf abscission is reduced to less than 10%, and most of plant after 20 days can clear-cutting forestland.Further increase the survival rate of Yunnan-Tibet lily magnolia introducing and planting.
Wherein in an embodiment, the concrete grammar of described Stratificated treatment is: be that 3-4:1 mix with the seed after vernalization process according to volume ratio by wet river sand, stratification 90-150 days at 15-25 DEG C.Preferably lamination under the natural temperature of introducing region.Conventional lamination is embedded in by seed in wet sand to be placed in 1-10 DEG C of environment, just can breaking dormancy effectively through the low temperature treatment of 1-3 month, but the present inventor finds, for Yunnan-Tibet lily magnolia seed, it is carried out lamination under introducing region (as Xuwen County) natural temperature, utilize the change of temperature within occurring in nature one day to realize alternating temperature stratification, there is higher seed germination rate on the contrary.And when lamination is stored, fine sand humidity is pinched with hand agglomerating, slowly can scatter when unclamping and be advisable.
Wherein in an embodiment, in described Stratificated treatment step, described wet river sand obtains by the following method: put into baking oven 90-120 DEG C of oven dry by after the river sand clear water of 1-3mm screen cloth is cleaned, naturally cool to room temperature, to obtain final product.Said method to carry out disinfection obtained wet river sand to river sand with baking, is particularly suitable for the lamination of Yunnan-Tibet lily magnolia seed, can improves seed germination rate.
Wherein in an embodiment, in described nursery step, described predetermined altitude is 1.2-1.5 rice.Because the seedling of Yunnan-Tibet lily magnolia is comparatively tender and lovely, therefore in order to protect seedling, improve survival rate, about growth of seedling to 1.2-1.5 rice need be treated, namely grow 1 year about 1 to 2 year, just seedling can be gone out garden.
Wherein in an embodiment, in described sowing step, be sowed at by the Seed Points after seed soaking in the Culture media be contained in container, the basal diameter of described container is more than or equal to 30cm, and the height of described container is more than or equal to 30cm.Because the growth time of seedling in container is longer, the container compared with large gauge should be selected, the optional non-woven bag of container material quality or polyethylene plastic bag etc.
Wherein in an embodiment, described Culture media is prepared by the following method: by bog moss: vermiculite: river sand: introducing region soil: the animal wastes of becoming thoroughly decomposed according to volume ratio be 0.8-1.2: 0.8-1.2: 0.8-1.2: 1.6-2.4: 0.8-1.2 mixing after, add superphosphate again, the addition of described superphosphate is the 1-3% of Culture media gross mass, again with the even spray disinfectant of the copperas solution of 1-3g/100ml, to obtain final product.Adopt above-mentioned Culture media, germination rate and the survival rate of Yunnan-Tibet lily magnolia can be improved.
Wherein in an embodiment, in described pre-treatment step, when the aggregate fruit of Yunnan-Tibet lily magnolia turns brown by aubergine, from initial uprightly become sagging time collecting fruit; And the indoor 2-3 days fruit collected spread out in shady and cool ventilation, aggregate fruit after-ripening is ftractureed, takes out seed; And this seed is continued at indoor airing 1-2 days, treat that exosper blackening or deliquescing are placed in clear water and soak 2-3 days, removing exosper, sterilization, for subsequent use.The routine disinfection mould clever pulvis of evil of 99% is dressed seed; Described seed is preferably 1000:5 with the mass ratio of disliking mould clever pulvis.Or be sprayed on sterilizing on seed with the mould Lingshui Spring dissolution homogeneity of the evil of dilution 1000 times.This sterilization method simple operations, is applicable to process seed in a large number, and object is to prevent from going mouldy.
Wherein in an embodiment, in described nursery step, respectively topdress once spring and autumn at seedling root.Bass long-acting sustained-release complex fertilizer (commercially available) preferably liked by the fertilizer used that topdresses.Further, in the pipe of nursery is supported, damage by disease and insect should with putting prevention first and combining prevention with control.
Compared with prior art, the present invention has following beneficial effect:
The introduction and cultivation method of a kind of Yunnan-Tibet of the present invention lily magnolia, by carrying out pre-treatment and vernalization to Yunnan-Tibet lily magnolia seed, and select specific solvent---acetone is used as the solvent carrier of vernalization hormone, improve the germination rate of Yunnan-Tibet lily magnolia seed, subsequently by this seed lamination in wet sand, with sowing after aqueous solution of salicylic acid seed soaking, and by the strict water content controlling Culture media, improve the emergence rate of Yunnan-Tibet lily magnolia seed, successfully High aititude will be originated in, high latitude, the Tibet that average annual temperature is lower, the seeds in Yunnan have introduced a fine variety low latitudes, ground height above sea level, the Xuwen County of mainland's southernmost end low latitudes that average annual temperature is higher.
Further, the method is also optimized wherein each step, by adopting the mode of alternating temperature stratification, improves seed germination rate (more than 85%) further; And by spraying aqueous solution of salicylic acid, probability seedling leaves come off is reduced to less than 10%; In order to improve survival rate further, also seedling is protected to 1.2-1.5 rice in shading shed, just carry out hardening, go out garden, substantially increase seedling percent.
Embodiment
For the ease of understanding the present invention, will be described more fully the present invention below.But the present invention can realize in many different forms, is not limited to embodiment described herein.On the contrary, provide the object of these embodiments be make the understanding of disclosure of the present invention more comprehensively thorough.
Unless otherwise defined, all technology used herein and scientific terminology are identical with belonging to the implication that those skilled in the art of the present invention understand usually.The object of term used in the description of the invention herein just in order to describe specific embodiment, is not intended to be restriction the present invention.
Embodiment 1
An introduction and cultivation method for Yunnan-Tibet lily magnolia, comprises the following steps:
One, pre-treatment.
1, gather.
Annual June-July, when the aggregate fruit of Yunnan-Tibet lily magnolia turns brown by aubergine, from initial uprightly become sagging time collecting fruit.
2, kind of a sterilization is got in airing.
The fruit collected is spread out the indoor 2-3 days in shady and cool ventilation, aggregate fruit after-ripening is ftractureed, take out seed; And this seed is continued at indoor airing 1-2 days, and stir every day, be strictly on guard against heating, treat that exosper blackening or deliquescing are placed in clear water and soak 2 days, rub removing exosper with warm buck (if pH is the sodium bicarbonate etc. of 8) or the washing agent (as soap etc.) of gentleness.
In order to prevent going mouldy, dress seed with the mould clever pulvis of evil; Described seed is preferably 1000:5 with the ratio of quality and the number of copies of disliking mould clever pulvis; Or be sprayed on sterilizing on seed with the mould Lingshui Spring dissolution homogeneity of the evil of dilution 1000 times.Yunnan-Tibet lily magnolia seed after sterilizing is for subsequent use.
Two, vernalization.
First by above-mentioned seed 2g/L liquor potassic permanganate surface sterilization 15 minutes, clean with clear water subsequently, soak seed 48 hours at normal temperatures, within every 24 hours, change a water, afterwards seed being soaked in GA content is process 48 hours in the acetone soln of 500-1500mg/L.
According to the difference of gibberellin acetone soln concentration, be divided into three groups, in three groups, the content of gibberellin is respectively 500mg/L, 1000mg/L, 1500mg/L.
Three, lamination.
Seed after above-mentioned vernalization process is carried out Stratificated treatment, and concrete grammar is as follows:
Baking oven (110 DEG C) hyperthermia drying is put into by after the thin river sand clear water of 2mm screen cloth is cleaned, naturally cool to room temperature, again by treated seed and wet river sand by volume 1:3-1:4 mix, when ensureing to store, fine sand humidity is pinched agglomerating with hand, slowly can scatter when unclamping and be advisable, natural stratification 90-150 days under the natural temperature (being about 15-25 DEG C) of Yunnan-Tibet lily magnolia plantation area.
Four, soak seed.
Getting the seed after lamination, after clear water washing and sterilizing, take concentration as the aqueous solution of salicylic acid seed soaking 24 hours of 0.5mmol/L.
Five, sow.
1, selection of container.
Container can select the material such as non-woven bag or polyethylene plastic bag, the container of specification comparatively large (basal diameter × high specification is greater than 30cm × 30cm).
2, Culture media preparation
By bog moss: vermiculite: river sand: introducing region soil: the animal wastes (the sheep excrement become thoroughly decomposed) of becoming thoroughly decomposed are 1: 1: 1: 2: 1 mixing according to volume ratio, and add superphosphate, the addition of described superphosphate is 2% of Culture media gross mass, sieve soil block, foreign material removing, again with the even spray disinfectant of the copperas solution of 2g/100ml, to obtain final product.
3, program request.
Seed Points after above-mentioned seed soaking is sowed at Culture media central authorities in container, the program request degree of depth is 0.5-2cm, and it covers one deck bog moss, and first time waters permeable.Should check the water content of soil matrix in Seed Germination every 5 days, and keep the skin wet in good time, the moisture mass percentage controlling Culture media is 20-25%, until seed sprouting.
Inventor finds, after planting when the water content of matrix is the highest to seed germination rate at 20%-25%, but when water content of substrate is more than 25%, percentage of seedgermination starts to decline.
Six, nursery.
After germination, it is bright and arid that seedling fears day with high temperature, and now seedling raising environment must be in shading shed, uses Shading net covering shading shed.When summer weather is overheated, as temperature more than 30 DEG C time, ensure that shading shed air flow moves with fan blower unimpeded, ensure the water supply of nursery stock, reduce the temperature in cool canopy.Further, the aqueous solution of salicylic acid of a 0.1mmol/L should be sprayed every 5-8 days.
Grow to after 1.2-1.5 rice (about cultivating a year and a half to two year) until seedling, the shade net of shading shed is progressively opened hardening to adapt to external condition, go out garden.
Cultivate Yunnan-Tibet lily magnolia according to above-mentioned introduction and cultivation method, and in the pregermination procedure of second step, select different vernalization solution, investigate contrast vernalization solvent to the impact of percentage of seedgermination.Often group gets 300 seeds, is divided into 3 parts, 100 every part, does 3 times and repeats, and in lamination step, all within the 100th day, get to plant in lamination and carry out seed germination experiment, add up it at the sowing percentage of seedgermination of the 20th day, result is as shown in the table.
The different vernalization solution of table 1. is on the impact of Yunnan-Tibet lily magnolia percentage of seedgermination
As can be seen from the above results, in lamination after 100 days, through the seed germination rate of gibberellin acetone soln process all more than 23%, be significantly higher than the gibberellin aqueous solution.And wherein reach the highest through the germination rate of the gibberellin acetone soln of 1500mg/L, reach to 32%.
Embodiment 2
An introduction and cultivation method for Yunnan-Tibet lily magnolia, substantially identical with the method for embodiment 1, difference is:
Three, lamination.
Adopt cool stratification and the lamination method described in embodiment 1 respectively, lamination is carried out to the seed after gibberellin acetone soln Steeping and budding.
The method of above-mentioned cool stratification is: by seed and wet river sand by volume 1:3-1:4 mix, stratification at 4 DEG C.
Investigate the percentage of seedgermination under different Stratification condition, concrete grammar is:
According to the method for above-mentioned cool stratification and embodiment 1 lamination, respectively lamination is carried out to Yunnan-Tibet lily magnolia seed, and respectively at not on the same day (30,60 of lamination, 90,110,130,150d), get 300, the seed of the Yunnan-Tibet lily magnolia of different gibberellin concentration seed soaking and Stratificated treatment, divide 3 parts, every part of 100 seeds, repeat as 3 times.It is placed in culture dish respectively, respectively uses two filter paper moisturizings up and down, be placed in 25 DEG C of insulating boxs and carry out vernalization cultivation, change 1 water every 2 days, add up its seed germination rate of the 20th day, result is as shown in the table.
The different vernalization of table 2. and Stratificated treatment are on the impact of Yunnan-Tibet lily magnolia percentage of seedgermination
As can be seen from the above results, in the primary stage (0-60d) of Stratificated treatment, the germination rate of seed is very low, and major part process germination rate is 0, is the highlyest no more than 10%.After Stratificated treatment 90d, the seed of each process all generally starts to germinate, and indicates that the seed of Yunnan-Tibet lily magnolia starts breaking dormancy.Along with the prolongation of Stratification time, reach about 70% via gibberellin acetone (1000mg/L and 1500mg/L) seed soaking and the embodiment 1 lamination method process seed germination rate of 130 days, the sleep procedure that now seed is described elementary solution is removed.The germination rate of seed continues to increase afterwards, and with the gibberellin acetone vernalization of 1500mg/L and the embodiment 1 lamination method best results of 150 days, germination rate can reach 84%.Under identical vernalization mode, the percentage of seedgermination outline after the process of embodiment 1 lamination method is higher than 4 DEG C of constant temperature process.
Embodiment 3
An introduction and cultivation method for Yunnan-Tibet lily magnolia, substantially identical with the method for embodiment 1, difference is:
Six, nursery.
Aqueous solution of salicylic acid is not used to spray seedling in the nursery step of the present embodiment.
By the introduction and cultivation method of the present embodiment compared with embodiment 1, specific experiment method is as follows:
Select consistent with embodiment 1, through the gibberellin acetone soln process vernalization of 1500mg/L, and via the Yunnan-Tibet lily magnolia seed of embodiment 1 lamination method lamination after 150 days.Totally 200, be divided into two groups, be respectively contrast groups and enforcement group, often organize 100 seeds.
The seed of contrast groups with Seed soaking process 24 hours, and in seedling raising process, when temperature is more than 30 DEG C, only to spray with clear water after lamination.
The seed of enforcement group is the aqueous solution of salicylic acid seed soaking 24 hours of 0.5mmol/L with concentration after lamination, and in seedling raising process, when temperature is more than 30 DEG C, sprays the aqueous solution of salicylic acid of a 0.1mmol/L every 5-8 days.
Result shows:
The seedling of control group, after temperature is more than 30 DEG C, first 10 days start to occur obvious poor growth, after 10 days, a large amount of seedling starts to engender jaundice, spot and curling phenomenon, and comes off with vaned, and cannot recover, after 30 days, the lethality of seedling reaches 85%.
And the seedling of enforcement group, after temperature is more than 30 DEG C, only the blade of part seedling has the performance of heat evil, and after 30 days, lethality is no more than 5%, and temperature drops to after below 30 DEG C, is all attended by the newborn cauline leaf of varying number, and recovery capability is strong.
Embodiment 4
An introduction and cultivation method for Yunnan-Tibet lily magnolia, substantially identical with the method for embodiment 1, difference is:
Six, nursery.
In the nursery step of the present embodiment, seedling after nursery 3-5 month, namely progressively removes shade net and carries out hardening, go out garden in a conventional manner in shading shed.
The introduction and cultivation method of the present embodiment is compared with embodiment 1, and because seedling goes out the garden time comparatively early, the survival rate in its later stage is lower, only can reach about 30%, and after the seedling of embodiment 1 goes out garden, survival rate can reach 90%.
Each technical characteristic of the above embodiment can combine arbitrarily, for making description succinct, the all possible combination of each technical characteristic in above-described embodiment is not all described, but, as long as the combination of these technical characteristics does not exist contradiction, be all considered to be the scope that this specification is recorded.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. an introduction and cultivation method for Yunnan-Tibet lily magnolia, is characterized in that, comprises the following steps:
Pre-treatment: the fruit gathering Yunnan-Tibet lily magnolia fruit ripe period, takes out seed after airing, removes seed coat oil film, obtains Yunnan-Tibet lily magnolia seed, for subsequent use;
Vernalization: it is process 24-72 hour in the acetone soln of 500-2000mg/L that above-mentioned Yunnan-Tibet lily magnolia seed is soaked in GA content;
Lamination: seed is embedded in wet sand and carries out Stratificated treatment;
Seed soaking: get the seed after lamination, after cleaning with clear water, take concentration as the aqueous solution of salicylic acid seed soaking 12-36 hour of 0.3-0.7mmol/L;
Sowing: be sowed in Culture media by the Seed Points after above-mentioned seed soaking, the program request degree of depth is 0.5-2cm, the moisture mass percentage controlling Culture media is 20-25%, until seed sprouting;
Nursery: be placed in shading shed by the seedling of germination, keeps ventilated, carries out nursery, grows to after predetermined altitude until seedling, the shade net of shading shed is opened hardening, goes out garden.
2. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, is characterized in that, in described presprouting of seeds step, first by Yunnan-Tibet lily magnolia seed at normal temperatures with water logging kind 24-72 hour, then this seed be placed in gibberellin acetone soln process.
3. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, is characterized in that, in described nursery step, when temperature is more than 30 DEG C, sprays the aqueous solution of salicylic acid of a 0.05-0.15mmol/L every 5-8 days.
4. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, it is characterized in that, the concrete grammar of described Stratificated treatment is: be that 3-4:1 mix with the seed after vernalization process according to volume ratio by wet river sand, stratification 90-150 days at 15-25 DEG C.
5. the introduction and cultivation method of Yunnan-Tibet according to claim 4 lily magnolia, it is characterized in that, in described Stratificated treatment step, described wet river sand obtains by the following method: put into baking oven 90-120 DEG C of oven dry by after the river sand clear water of 1-3mm screen cloth is cleaned, naturally cool to room temperature, to obtain final product.
6. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, is characterized in that, in described nursery step, described predetermined altitude is 1.2-1.5 rice.
7. the introduction and cultivation method of Yunnan-Tibet according to claim 6 lily magnolia, it is characterized in that, in described sowing step, Seed Points after seed soaking is sowed in the Culture media be contained in container, the basal diameter of described container is more than or equal to 30cm, and the height of described container is more than or equal to 30cm.
8. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, it is characterized in that, described Culture media is prepared by the following method: by bog moss: vermiculite: river sand: introducing region soil: the animal wastes of becoming thoroughly decomposed according to volume ratio be 0.8-1.2: 0.8-1.2: 0.8-1.2: 1.6-2.4: 0.8-1.2 mixing after, add superphosphate again, the addition of described superphosphate is the 1-3% of Culture media gross mass, again with the even spray disinfectant of the copperas solution of 1-3g/100ml, to obtain final product.
9. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, is characterized in that, in described pre-treatment step, when the aggregate fruit of Yunnan-Tibet lily magnolia turns brown by aubergine, from initial uprightly become sagging time collecting fruit; And the indoor 2-3 days fruit collected spread out in shady and cool ventilation, aggregate fruit after-ripening is ftractureed, takes out seed; And this seed is continued at indoor airing 1-2 days, treat that exosper blackening or deliquescing are placed in clear water and soak 2-3 days, removing exosper, sterilization, for subsequent use.
10. the introduction and cultivation method of Yunnan-Tibet according to claim 1 lily magnolia, is characterized in that, in described nursery step, respectively topdresses once spring and autumn at seedling root.
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| CN116848992B (en) * | 2023-09-05 | 2023-11-10 | 中国科学院昆明植物研究所 | Quick germination and breeding method for yulan yunnanensis seeds |
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