CN101785382A - Method for promoting sprouting of clematis glauca seed - Google Patents
Method for promoting sprouting of clematis glauca seed Download PDFInfo
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- CN101785382A CN101785382A CN 201010119747 CN201010119747A CN101785382A CN 101785382 A CN101785382 A CN 101785382A CN 201010119747 CN201010119747 CN 201010119747 CN 201010119747 A CN201010119747 A CN 201010119747A CN 101785382 A CN101785382 A CN 101785382A
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- benzyladenine
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Abstract
The invention discloses a method for promoting the sprouting of a clematis glauca seed, which is characterized by comprising the following steps of: (1) respectively preparing 100-300mg/L of gibberellin solution and20-40mg/L of 6-benzyladenine solution by adopting acetone as a solvent; (2) soaking the clematis glauca seed in 0.1 percent of potassium permanganate solution for one hour; soaking the clematis glauca seed by using the gibberellin or 6-benzyladenine solution for one hour and then placing in a refrigerator at 9 DEG C for refrigerating for one month; and (3) placing the refrigerated seed into a thermostat at 25 DEG C for promoting sprouting. The method has obvious function of promoting the sprouting of the seed by utilizing the gibberellin and the 6-benzyladenine, the seed sprouting rates respectively reach 64 percent and 67 percent, and the sprouting characteristics of the seed are improved.
Description
Technical field
The present invention relates to a kind of method that promotes seed sprouting, relate to the germinating method of clematis glauca specifically, belong to agricultural technology field.
Background technology
Clematis glauca (Clematis glauca Willd.Herd.Baumz.) is a Ranunculaceae Clematis liane, one to two time winglike compound leaf, full edge or the minority tooth is arranged.Normal is uniparous cyme, 3 flowers, and bract is lobate, and full edge or 2-3 split sepal 4, yellow.Florescence 6, really August phase was to October to July.Adopt seed soaking, drying, low temperature, potassium permanganate, gibberellin, ultrasonic etc. to handle Chinese pulsatilla seed, measure seed germinating time, germination rate, germination vigor.The result shows: Chinese pulsatilla seed has the embryo dormancy characteristic.Gibberellin 100,50,20mg/L and refrigeration can significantly improve Chinese pulsatilla seed germination rate, germination vigor.(Shi Weijing, Zhang Zixue, in heroes .2005, Chinese pulsatilla seed sprouting characteristic research. seed .24 (4): 60-62)
Handle seed with plant growth regulator, improve seed vitality, strengthen the emerge germination rate of seed in adverse circumstance, the treatment technology of plant seedlings being controlled prosperous short weak, strong seedling strong sprout is well used (Wang Jichuan on various vegetables, fruit plant, the positive magnificent .2000 of poplar, growth regulator is to the influence of wheat seeds sprouting and growth of seedling. seed science and technology. (5): 281-283.).
The seed of horseradish and caragana also all uses the 6-benzyladenine of variable concentrations and gibberellin treatment to improve germination rate, received effect (Cui Cui preferably, He Fengfa, Zhou Qingyuan .2005,6-benzyladenine and gibberellin are to the influence of horseradish seed germination. Agricultural University Of Southwest's journal (natural science edition) .27 (1): 78-80 paddy pellet, Wang Jianhua .2004, gibberellin and 6-benzyladenine are to caragana microphylla caragana seed germination and growth of seedling study on regulation. seed, 23 (11): 3-6)
Research vernalization, chemicals treatment, treatment of different temperature are to the influence of intricate clematis herb (Clematisintricate) seed germination characteristic.The result shows that percentage of seedgermination and contrast difference are extremely remarkable behind 4 ℃ of cryogenic conditions vernalization 14d, and germination rate is the highest; Under condition of different temperatures, it is extremely remarkable that each handles percentage of seedgermination difference, and the suitable sprouting of seed temperature is 20 ℃; After using 50mg/L concentration Processing Test material, percentage of seedgermination and contrast difference are extremely remarkable; Ground height above sea level rises different acquisition ground percentage of seedgermination, year samming decline increases with gathering.(Wei Weidong, the glad .2008 of horse, Qinghai resource plant intricate clematis herb seed germination characteristic research. resource and utilization, (27) 3:49-51)
Kindred plant seed structure is not different, and complex physical is taking place during germination to be changed, and the also different (Pan Ruichi of its germination physiological property of different plant, Dong Yu gets. plant physiology, Higher Education Publishing House, 1998), yet there are no report for the clematis glauca seed treatment.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method that promotes sprouting of clematis glauca seed.
For solving the problems of the technologies described above, the invention provides a kind of method that promotes sprouting of clematis glauca seed, it is characterized in that, may further comprise the steps:
1) is solvent with acetone respectively, is mixed with the Gibberellins solution of 100-300mg/L and the 6-benzyladenine solution of 20-40mg/L;
2) the clematis glauca seed was soaked 1 hour in 0.1% liquor potassic permanganate, again the clematis glauca seed is put into 9 ℃ of refrigerator and cooled with immersion in gibberellin or the 6-benzyladenine solution after 1 hour and hide January;
3) seed after the refrigeration is put into 25 ℃ of insulating box vernalization.
The method of aforesaid promotion sprouting of clematis glauca seed is characterized in that: the concentration of described Gibberellins solution is 100mg/L, 200mg/L or 300mg/L, and the concentration of 6-benzyladenine solution is 20mg/L, 30mg/L or 40mg/L.
The beneficial effect that the present invention reached:
Method of the present invention utilizes gibberellin and 6-benzyladenine to promote that the sprouting effect of seed is obvious, and percentage of seedgermination reaches 64% and 67% respectively, has improved the germination characteristic of seed.
Description of drawings
Fig. 1 is the curve map of gibberellin among the present invention to the influence of green clematis seed sprouting;
Fig. 2 is the curve map of 6-benzyladenine among the present invention to the influence of green clematis seed sprouting.
Embodiment
Clematis plant original seed generally adopts the method for seminal propagation.Use gibberellin (gibberellin), 6-benzyladenine (6-benzyladenine) two plant growth regulators of multiple concentration that the clematis seed is handled in the present embodiment respectively, be intended to explore of the influence of this two plant growth regulators, provide foundation in the hope of breeding research for the preservation of wild clematis plant resources, introducing and planting and Clematis plant to the seed germination characteristic of clematis.
For the examination material
The clematis glauca seed picks up from Urumchi, Xinjiang board house ditch.Thousand kernel weight is 1.56g.
Growth regulator: GA content is 92%, and 6-benzyladenine content is 99.6%, available from Beijing chemical reagents corporation.
The preparation of reagent
Be solvent with acetone respectively, be mixed with 1001mg/L, 200mg/L, the Gibberellins solution of 300mg/L and 20mg/L, 30mg/L, the 6-benzyladenine solution of 40mg/L.
Seed treatment
Seed detects through naked eyes, removes brokenly grain and worm-eaten seed.See Table 1 for the clematis glauca seed treatment, table 1 is the test of significance result of the germination characteristic of clematis glauca under the different disposal, all soaks 1 hour in 0.1% liquor potassic permanganate before.Contrast is for soaking 1 hour in the clear water kind, and other are handled all and soaked 1 hour in the plant growth regulator of variable concentrations.
Table 1
Hide putting into 9 ℃ of refrigerator and cooled after the seed treatment, refrigeration is taken out after January and is put into 25 ℃ of insulating box vernalization.Every processing repeats 3 times, and 100 seeds of every repetition are put into the 10cm * 10cm culture dish that is covered with 2 metafiltration paper according to 10 * 10, and moisturizing day by day.
May 26, seed began germination (germinating time among Fig. 1, Fig. 2 was counted from this moment), it is normal chitting piece that the seeds germinated bud reaches seed length, duration of germination is checked every day, record seed sprouting grain number, sowing simultaneously, finish to germinateing, statistics germination rate, germination vigor and emergence rate also carries out variance analysis.
The total grain of chitting piece number in germination vigor (%)=alloted days/experiment number * 100%
The total grain of the total grain of germination rate (%)=germination number/experiment number * 100%
Emergence rate (the %)=total grain of number/germination number * 100% of emerging
Sowing media is a peat soil, and broadcasting into every cave diameter is 5 centimetres, is in 10 centimetres the cave dish deeply.Broadcasting the back waters every day.The statistics emergence rate.
Gibberellin (100mg/L) and 6-benzyladenine (30mg/L) promote that the sprouting effect of seed is the most obvious, and percentage of seedgermination reaches 64% and 67% respectively, has improved the germination characteristic of seed.
Different disposal is to the influence of clematis percentage of seedgermination
Fig. 1 shows that the peak of the germination rate of every day all is higher than contrast behind the gibberellin treatment of all concentration, and general trend is that germination rate descends gradually; After putting into 25 ℃ of insulating boxs, gibberellin 00, GA100 and contrast all begin to sprout in the 34th (1 in the abscissa) sky, GA200 begins to sprout in the 35th (2 in the abscissa) sky, the 46th (13 in a abscissa) day germination is all over, the germination rate top that gibberellin 00 and GA100 handle was at first day, after this gibberellin 00 has two little peaks again in the 38th (5 in the abscissa) sky and the 39th (8 in the abscissa) sky, GA100 has occurred little peak in the 37th, 38 (4 in the abscissas, 5) sky, and GA200 germination rate top was at the 37th, 38 day.The contrast germination process is more steady, does not see tangible peak.
Fig. 2 as can be seen, the peak that the 6-benzyladenine of all concentration is handled germination rate every day also all is higher than contrast.Germination rate peak after wherein 6-benzyladenine 40,6-benzyladenine 30 and 6-benzyladenine 20 are handled is all at the 3rd day, and the germination rate curve of preceding two processing is almost overlapping.But the germination rate general trend is by day decline.This is just opposite with Chinese pulsatilla percentage of seedgermination variation tendency.
More than the reasonable concentration of explanation plant growth regulator can significantly improve the clematis seed germination rate.
Below disclose the present invention with preferred embodiment, so it is not in order to restriction the present invention, and all employings are equal to replaces or technical scheme that the equivalent transformation mode is obtained, all drops within protection scope of the present invention.
Claims (2)
1. a method that promotes sprouting of clematis glauca seed is characterized in that, may further comprise the steps:
1) is solvent with acetone respectively, is mixed with the Gibberellins solution of 100-300mg/L and the 6-benzyladenine solution of 20-40mg/L;
2) the clematis glauca seed was soaked 1 hour in 0.1% liquor potassic permanganate, again the clematis glauca seed is put into 9 ℃ of refrigerator and cooled with immersion in gibberellin or the 6-benzyladenine solution after 1 hour and hide January;
3) seed after the refrigeration is put into 25 ℃ of insulating box vernalization.
2. the method for promotion sprouting of clematis glauca seed according to claim 1 is characterized in that: the concentration of described Gibberellins solution is 100mg/L, 200mg/L or 300mg/L, and the concentration of 6-benzyladenine solution is 20mg/L, 30mg/L or 40mg/L.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102598917A (en) * | 2012-03-30 | 2012-07-25 | 常熟市新靓文辅亚农艺发展有限公司 | Cultivation method for breaking through woodbine seed dormancy and improving survival rate of seedlings |
| CN104920198A (en) * | 2015-07-14 | 2015-09-23 | 安徽省应用技术研究院 | Seed germination acceleration seedling culturing method for polygonatum cyrtonema |
| CN105409518A (en) * | 2015-11-23 | 2016-03-23 | 广东神州木兰园林有限公司 | Introduction and cultivation method for campbell's magnolia |
| CN107567760A (en) * | 2017-09-29 | 2018-01-12 | 江苏农林职业技术学院 | A kind of method for improving long flower clematis percentage of seedgermination and emergence rate |
| CN107580822A (en) * | 2017-09-29 | 2018-01-16 | 江苏农林职业技术学院 | A kind of method for improving bastard clematis percentage of seedgermination and emergence rate |
| CN107864713A (en) * | 2017-11-18 | 2018-04-03 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method for accelerating China tree germination |
| CN109287427A (en) * | 2018-09-19 | 2019-02-01 | 浙江省亚热带作物研究所 | A kind of method of the clematis production of hybrid seeds and seedling breeding |
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| CN1868263A (en) * | 2006-06-29 | 2006-11-29 | 南京林业大学 | Tissue culture method for Clematis Multi Blue |
| CN101361439A (en) * | 2008-09-12 | 2009-02-11 | 浙江大东吴药业有限公司 | Cultivation method of sweetautumn clematis |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1868263A (en) * | 2006-06-29 | 2006-11-29 | 南京林业大学 | Tissue culture method for Clematis Multi Blue |
| CN101361439A (en) * | 2008-09-12 | 2009-02-11 | 浙江大东吴药业有限公司 | Cultivation method of sweetautumn clematis |
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| 《新疆农垦科技》 20091231 冯兴林 观赏药用植物-铁线莲 第14-15页 1-2 , 第4期 2 * |
| 《种子》 19921231 顾增辉等 应用赤霉素有机溶剂渗入法打破花卉种子休眠和促进萌发 第56-57页第"2.铁线莲属种子的试验"部分 1-2 , 第1期 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102598917A (en) * | 2012-03-30 | 2012-07-25 | 常熟市新靓文辅亚农艺发展有限公司 | Cultivation method for breaking through woodbine seed dormancy and improving survival rate of seedlings |
| CN104920198A (en) * | 2015-07-14 | 2015-09-23 | 安徽省应用技术研究院 | Seed germination acceleration seedling culturing method for polygonatum cyrtonema |
| CN105409518A (en) * | 2015-11-23 | 2016-03-23 | 广东神州木兰园林有限公司 | Introduction and cultivation method for campbell's magnolia |
| CN105409518B (en) * | 2015-11-23 | 2018-05-15 | 广东神州木兰园林有限公司 | The introduction and cultivation method of Yunnan-Tibet lily magnolia |
| CN107567760A (en) * | 2017-09-29 | 2018-01-12 | 江苏农林职业技术学院 | A kind of method for improving long flower clematis percentage of seedgermination and emergence rate |
| CN107580822A (en) * | 2017-09-29 | 2018-01-16 | 江苏农林职业技术学院 | A kind of method for improving bastard clematis percentage of seedgermination and emergence rate |
| CN107864713A (en) * | 2017-11-18 | 2018-04-03 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method for accelerating China tree germination |
| CN109287427A (en) * | 2018-09-19 | 2019-02-01 | 浙江省亚热带作物研究所 | A kind of method of the clematis production of hybrid seeds and seedling breeding |
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